The present study shows the advantage of the palynostratigraphy and paleoenvironmental inferences concepts in subdividing and interpreting the depositional environment of the Jurassic rocks, depending on two sections in two boreholes drilled in the Darag Basin, Egypt. Six Jurassic rock units representing the mixed siliciclastic and carbonate sediments were recognized from top to base; the Tauriat, Arousiah, Kehailia, Safa, Shusha and Rajabiah formations. The palynomorph assemblage associated with moderately diversified dinoflagellate cysts and fewer sporomorphs, show poor to fair preservation. About 20 dinoflagellate cysts, 8 pollen, and 3 pteridophytic spore species have been recorded. The Jurassic rocks are originated from the late Jurassic (early Oxfordian) and early (Toarcian - Aaleneian) based on the recovered marker taxa. The studied sections are differentiated into six biozones, Systematophora sp. (Callovian-Oxfordian), Gonyaulacysta jurassica (Callovian), Dichadogonyaulax sellwoodii (Late Bathonian), Sentusidinium sp. (Early Bathonian), Pareodinia ceratophora (Bajocian) and Psilate spores, Classopollis and Sphaeripollenites Acme Zone (Toarcian-Aalenian).According to the Particulate Organic Matter (POM) composition in the studied wells, the palynofacies assemblages led to identifying three depositional settings, the palynofacies −1 (PFA-1), which is characterized by opaque phytoclasts and high volume of spores and pollen grains was deposited in a marginal shallow-marine environment during a relative sea-level fall. The PFA-2 is composed of brown phytoclasts, moderate Amorphous Organic Matter (AOM) with pollen grains and dominated dinocysts. It was deposited during a slight sea level rise in a proximal inner neritic environment. Simultaneously, the PFA-3, which was dominated by AOM and dinoflagellate cysts with moderate brown phytoclast content, was deposited as a distal inner neritic environment during a relatively high relative sea level.