This study was performed to clarify criteria for cavitation inception and the relationship between flow conditions and cavitation flow patterns of diesel and biodiesel fuels. The goal was to analyze the effects of injection conditions and fuel properties on cavitating flow and disintegration phenomena of flow after fuel injection. To accomplish this goal, it was utilized a test nozzle with a cylindrical cross-sectional orifice and a flow visualization system composed of a fuel supply system and an image acquisition system. In order to analyze the rate of flow and injection pressure of the fuel, a flow rate meter and pressure gauge were installed at the entrance of the nozzle. A long distance microscope device equipped with a digital camera and a high resolution ICCD camera were used to acquire flow images of diesel and biodiesel, respectively. The effects of nozzle geometry on the cavitating flow were also investigated. Lastly, a detailed comparison of the nozzle cavitation characteristics of both fuel types was conducted under a variety of fuel injection parameters. The results of this analysis revealed that nozzle cavitation flow could be divided into four regimes: turbulent flow, beginning of cavitation, growth of cavitation, and hydraulic flip. The velocity coefficient of diesel fuel was greatly altered following an increase in flow rate, although for biodiesel, the variation of the velocity coefficient relative to the rate of flow was mostly constant. The cavitation number decreased gradually with an increase in the Reynolds number and Weber number, and the discharge coefficient was nearly equal to one, regardless of cavitation number. Lastly, it could not observe cavitation growth in the tapered nozzle despite an increase in fuel injection pressure.