Cyanotoxin Concentrations Research Articles

High concentrations of cyanotoxins in reservoirs in the State of São Paulo (Brazil) may present a risk to public health

High concentrations of cyanotoxins in reservoirs in the State of São Paulo (Brazil) may present a risk to public health

Effects of Harmful Algal Blooms on Amphibians and Reptiles are Under-Reported and Under-Represented.

Harmful algal blooms (HABs) are a persistent and increasing problem globally, yet we still have limited knowledge about how they affect wildlife. Although semi-aquatic and aquatic amphibians and reptiles have experienced large declines and occupy environments where HABs are increasingly problematic, their vulnerability to HABs remains unclear. To inform monitoring, management, and future research, we conducted a literature review, synthesized the studies, and report on the mortality events describing effects of cyanotoxins from HABs on freshwater herpetofauna. Our review identified 37 unique studies and 71 endpoints (no-observed-effect and lowest-observed-effect concentrations) involving 11 amphibian and 3 reptile species worldwide. Responses varied widely among studies, species, and exposure concentrations used in experiments. Concentrations causing lethal and sublethal effects in laboratory experiments were generally 1 to 100 µg/L, which contains the mean value of reported HAB events but is 70 times less than the maximum cyanotoxin concentrations reported in the environment. However, one species of amphibian was tolerant to concentrations of 10,000 µg/L, demonstrating potentially immense differences in sensitivities. Most studies focused on microcystin-LR (MC-LR), which can increase systemic inflammation and harm the digestive system, reproductive organs, liver, kidneys, and development. The few studies on other cyanotoxins illustrated that effects resembled those of MC-LR at similar concentrations, but more research is needed to describe effects of other cyanotoxins and mixtures of cyanotoxins that commonly occur in the environment. All experimental studies were on larval and adult amphibians; there were no such studies on reptiles. Experimental work with reptiles and adult amphibians is needed to clarify thresholds of tolerance. Only nine mortality events were reported, mostly for reptiles. Given that amphibians likely decay faster than reptiles, which have tissues that resist decomposition, mass amphibian mortality events from HABs have likely been under-reported. We propose that future efforts should be focused on seven major areas, to enhance our understanding of effects and monitoring of HABs on herpetofauna that fill important roles in freshwater and terrestrial environments. Environ Toxicol Chem 2024;43:1936-1949. Published 2024. This article is a U.S. Government work and is in the public domain in the USA. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Untargeted LC-HRMS applied to microcystin-producing cyanobacterial cultures for the evaluation of the efficiency of chlorine-based treatments commonly used for water potabilization

Cyanobacteria in water supplies are considered an emerging threat, as some species produce toxic metabolites, cyanotoxins, of which the most widespread and well-studied are microcystins. Consumption of contaminated water is a common exposure route to cyanotoxins, making the study of cyanobacteria in drinking waters a priority to protect public health. In drinking water treatment plants, pre-oxidation with chlorinated compounds is widely employed to inhibit cyanobacterial growth, although concerns on its efficacy in reducing cyanotoxin content exists. Additionally, the effects of chlorination on abundant but less-studied cyanometabolites (e.g. cyanopeptolins whose toxicity is still unclear) remain poorly investigated. Here, two chlorinated oxidants, sodium hypochlorite (NaClO) and chlorine dioxide (ClO2), were tested on the toxic cyanobacterium Microcystis aeruginosa, evaluating their effect on cell viability, toxin profile and content. Intra- and extracellular microcystins and other cyanometabolites, including their degradation products, were identified using an untargeted LC-HRMS approach. Both oxidants were able to inactivate M. aeruginosa cells at a low dose (0.5 mg L−1), and greatly reduced intracellular toxins content (>90%), regardless of the treatment time (1–3 h). Conversely, a two-fold increase of extracellular toxins after NaClO treatment emerged, suggesting a cellular damage. A novel metabolite named cyanopeptolin-type peptide-1029, was identified based on LC-HRMSn (n = 2, 3) evidence, and it was differently affected by the two oxidants. NaClO led to increase its extracellular concentration from 2 to 80–100 μg L−1, and ClO2 induced the formation of its oxidized derivative, cyanopeptolin-type peptide-1045. In conclusion, pre-oxidation treatments of raw water contaminated by toxic cyanobacteria may lead to increased cyanotoxin concentrations in drinking water and, depending on the chemical agent, its dose and treatment duration, also of oxidized metabolites. Since the effects of such metabolites on human health remain unknown, this issue should be handled with extreme caution by water security agencies involved in drinking water management.

The short-term effect of nitrogen on freshwater cyanobacteria and cyanotoxins

Toxic cyanobacterial blooms are a worldwide concern. Nutrients are among the numerous factors that trigger cyanobacterial blooms and the production of cyanotoxins. This study aimed to assess the effect of nitrogen on cyanobacteria and cyanotoxins through a short-term mesocosm experiment. To achieve this, mesocosms were installed in situ in two lakes (the Missisquoi Bay of Lake Champlain and Petit-Lac-St-François) and received 700 μg/L of ammonium or 500 μg/L of nitrate. A shift to Microcystis in the mesocosms with nitrogen addition was observed 2 days after the addition in Missisquoi Bay, where nitrogen was more limited than Petit-Lac-St-François based on nutrient ratios. There was a significant increase of extracellular ΣMCs and MC-LR in mesocosms with the addition of ammonium or nitrate after 48 h compared to control mesocosms. Intracellular and extracellular microcystin concentrations were associated with Microcystis. Microcystis presence was associated with ammonium 48 h after N was added at both sites. Therefore, toxin production following sudden nitrogen addition can occur on short time scales relevant to drinking water treatment plant operations. This information will help treatment plant operators better anticipate the arrival of potentially higher concentrations of cyanotoxins.

Evaluation of metrics and thresholds for use in national-scale river harmful algal bloom assessments

The spatiotemporal distribution of harmful algal blooms (HABs) in rivers remains poorly understood, and there is an urgent need to develop a consistent set of metrics to better document HAB occurrences and forecast future events. Using data from seven sites in the Illinois River Basin, we computed metrics focused on HAB conditions related to excess algal growth and hypoxia. Daily mean chlorophyll and dissolved oxygen (DO) concentrations, gross primary productivity (GPP), and net ecosystem productivity (NEP) rates, focused on water quality status, identifying the timing of the transition from a clear-water to an algal dominated state. Early warning indicators (EWIs), the first-order autoregressive process (Ar1) and standard deviation (SD) of chlorophyll concentrations, focused on future events, forecasting blooms. Metrics were compared to either literature-derived or statistical-based thresholds and were normalized by total number of daily samples for an exceedance rate. Exceedances of a daily mean chlorophyll concentration averaged 50 % across all sites using a 10 µg L−1 threshold but increasing the threshold to 50 μg L−1 reduced the average exceedance rate to 5 %. The average exceedance rate for GPP (∼8 g O2 m2d−1 threshold) was 15 %, similar to the daily amplitude DO concentration (∼3 mg L−1 threshold), but the average for NEP (0 g O2 m2 d−1 threshold) was higher, at 28 %. The number of days with at least 1 continuous DO concentration below the threshold of 5, 3, or 2 mg L−1, had basin wide exceedance rates of 9 %, 3 %, and 2 %, respectively. Thresholds for EWIs, Ar1 and SD, were exceeded at 5 of the 7 sites with high chlorophyll concentrations and GPP rates. The correlation between proxies for algal biomass (chlorophyll concentration) and productivity (GPP) was strongest for sites in the middle region of the basin, with R2 values between 0.54 and 0.74. Although, cyanotoxin concentrations are the most commonly used metrics by states to define an inland water HAB, there is a paucity of publicly available data. The wider availability of chlorophyll and oxygen data combined with the results from this study suggest that biomass and productivity state and event-based metrics may be a promising way to assess and predict the vulnerability of rivers to some of the deleterious effects of HABs at broad spatial scales.

Harmful blooms across a longitudinal gradient in central Europe during heatwave: Cyanobacteria biomass, cyanotoxins, and nutrients

Climate change has increased the frequency, duration and intensity of heatwaves in Europe. These extreme events result in alterations of physical, chemical, and biological properties of lakes that may synergistically promote cyanobacterial dominance. In our study we focused on cyanobacterial blooms in lakes distributed over a longitudinal gradient in Central Europe during one of the “top ten European heat waves” in summer 2015. 92 lakes were included in the study, located across three climatic subregions: cool northern lakes, situated in Lithuania, temperate northern lakes in Poland, and warm northern lakes in Croatia. The objective of the study was to determine if cyanobacterial biomass, predominant species, and cyanotoxin concentration differed, across the south-north gradient, as a function of water temperature, total phosphorus, and total nitrogen. Statistical significance of observed patterns was tested using the Kruskal-Wallis rank sum test and the generalized linear model. We found the lowest average epilimnion temperature, but the highest average cyanobacterial biomass in the northern, ‘cool’ lakes while the highest average temperature with the lowest average cyanobacterial biomass in the southern, ‘warm’ lakes. The concentration of cyanotoxins was also the highest in the ‘cool’ lakes. Total phosphorus and total nitrogen correlated significantly with cyanobacterial biomass, cyanotoxins concentration and biomass of some cyanobacterial species (mainly Planktothrix agardhii), regardless of the latitude. Only in the ‘cool’ lakes concentration of cyanotoxins (microcystins and anatoxin-a) correlated significantly with cyanobacterial biomass and the biomass of some dominant cyanobacterial species (P. agardhii). Our results emphasized the differences of heat weaves impact on lakes of various latitudes, with the strongest increase in toxic cyanobacterial blooms in northern ‘cool’ lakes, situated in high latitudes. On the other hand, nutrients directly enhanced blooms across all the studied latitudes of Central Europe. The cyanobacteria species dominating in blooms might be recognized as ecological indicators of climate change, especially in the north-eastern part of Europe.

Shifts in phytoplankton and zooplankton communities in three cyanobacteria-dominated lakes after treatment with hydrogen peroxide

Cyanobacteria can reach high densities in eutrophic lakes, which may cause problems due to their potential toxin production. Several methods are in use to prevent, control or mitigate harmful cyanobacterial blooms. Treatment of blooms with low concentrations of hydrogen peroxide (H2O2) is a promising emergency method. However, effects of H2O2 on cyanobacteria, eukaryotic phytoplankton and zooplankton have mainly been studied in controlled cultures and mesocosm experiments, while much less is known about the effectiveness and potential side effects of H2O2 treatments on entire lake ecosystems. In this study, we report on three different lakes in the Netherlands that were treated with average H2O2 concentrations ranging from 2 to 5 mg L−1 to suppress cyanobacterial blooms. Effects on phytoplankton and zooplankton communities, on cyanotoxin concentrations, and on nutrient availability in the lakes were assessed. After every H2O2 treatment, cyanobacteria drastically declined, sometimes by more than 99%, although blooms of Dolichospermum sp., Aphanizomenon sp., and Planktothrix rubescens were more strongly suppressed than a Planktothrix agardhii bloom. Eukaryotic phytoplankton were not significantly affected by the H2O2 additions and had an initial advantage over cyanobacteria after the treatment, when ample nutrients and light were available. In all three lakes, a new cyanobacterial bloom developed within several weeks after the first H2O2 treatment, and in two lakes a second H2O2 treatment was therefore applied to again suppress the cyanobacterial population. Rotifers strongly declined after most H2O2 treatments except when the H2O2 concentration was ≤ 2 mg L−1, whereas cladocerans were only mildly affected and copepods were least impacted by the added H2O2. In response to the treatments, the cyanotoxins microcystins and anabaenopeptins were released from the cells into the water column, but disappeared after a few days. We conclude that lake treatments with low concentrations of H2O2 can be a successful tool to suppress harmful cyanobacterial blooms, but may negatively affect some of the zooplankton taxa in lakes. We advise pre-tests prior to the treatment of lakes to define optimal treatment concentrations that kill the majority of the cyanobacteria and to minimize potential side effects on non-target organisms. In some cases, the pre-tests may discourage treatment of the lake.

Removal of a cyanotoxins mixture by loose nanofiltration membranes applied in drinking water production

Cyanobacterial toxins may threaten human health if their levels in drinking water exceed certain thresholds. Therefore, it is important for water works that use raw water sources prone to cyanobacterial blooms to have efficient barriers against such toxins. Nanofiltration (NF) is one potential barrier. The efficacy and mechanism of removing four cyanotoxins, namely microcystins (MCs), cylindrospermopsin (CYN), saxitoxins (STXs), and anatoxin (ATX), were studied at bench-scale using NF membranes commonly applied in Norwegian drinking water facilities. The average removal of the different cyanotoxins under the tested operating conditions ranged from 15 % to 96 %. The membrane with the lowest molecular weight cut-off (MWCO) of 0.3 kDa made of polyamide (PA) was deemed the most suitable for the removal of all studied cyanotoxins. A gradual improvement of rejection observed with the 2 kDa cellulose acetate (CA) membrane was linked to the formation of fouling on the membrane surface. Sulfonated polyethersulfone (SPES) membranes with MWCO of 1 and 3 kDa could not efficiently and consistently remove cyanotoxins, except for MCs. The rejection of MCs over time was over 80 % by the SPES membranes during two days of filtration. The influence of pressure and pH as relevant operating parameters was evaluated. However, the analysis of the cyanotoxin concentrations in the permeate indicated that the investigated NF membranes alone would generally not be able to meet the WHO guidelines for drinking water during a severe cyanobacterial bloom. Thus, incorporating other water treatment technologies should be considered to effectively remove cyanotoxins.

Cyanotoxins in African waterbodies: occurrence, adverse effects, and potential risk to animal and human health.

Public concerns about cyanotoxins production in water and its detrimental impacts on human and animal health are growing primarily due to the widespread eutrophication observed in aquatic ecosystems. A review of relevant literature was done to determine the degree of cyanotoxin occurrence and its harmful effects in African waterbodies. Data were extracted from 64 published studies from 1990 to 2022 that quantified the concentration of cyanotoxins in African aquatic ecosystems. Cyanotoxins have been reported in 95 waterbodies (29 lakes, 41 reservoirs, 10 ponds, 9 rivers, 5 coastal waters, and 1 irrigation canal) from 15 African countries. Cyanotoxins were documented in all the regions of Africa except the central region. Microcystins have been reported in nearly all waterbodies (98.9%), but anatoxin-a (5.3%), cylindrospermopsin (2.1%), nodularins (2.1%), homoanatoxin-a (1.1%), and β-N-methylamino-L-alanine (1.1%) were encountered in a small number of water ecosystems, homoanatoxin-a and β-N-methylamino-L-alanine each occurred in one waterbody. The largest concentrations of microcystins and nodularins were reported in South African Lakes Nhlanganzwani (49,410μgL-1) and Zeekoevlei (347,000μgg-1). Microcystin concentrations exceeding the WHO guideline for lifetime drinking water (1μgL-1) were reported in 63% of the aquatic ecosystems surveyed. The most frequently reported toxin-producing cyanobacteria genus is Microcystis spp. (73.7%), followed by Oscillatoria spp. (35.8%) and Dolichospermum spp. (33.7%). Cyanotoxin-related animal mortality and human illness were reported in the continent. Consequently, it is necessary to regularly monitor the level of nutrients, cyanobacteria, and cyanotoxins in African waterbodies in an integrated manner to devise a sustainable water resources management.

Predicting water quality variability in a Mediterranean hypereutrophic monomictic reservoir using Sentinel 2 MSI: the importance of considering model functional form.

Anthropogenic eutrophication is a global environmental problem threatening the ecological functions of many inland freshwaters and diminishing their abilities to meet their designated uses. Water authorities worldwide are being pressed to improve their abilities to monitor, predict, and manage the incidence of harmful algal blooms (HABs). While most water quality management decisions are still based on conventional monitoring programs that lack the needed spatio-temporal resolution for effective lake/reservoir management, recent advances in remote sensing are providing new opportunities towards better understanding water quality variability in these important freshwater systems. This study assessed the potential of using the Sentinel 2 Multispectral Instrument to predict and assess the spatio-temporal variability in the water quality of the Qaraoun Reservoir, a poorly monitored Mediterranean hypereutrophic monomictic reservoir that is subject to extensive periods of HABs. The work first evaluated the ability to transfer and recalibrate previously developed reservoir-specific Landsat 7 and 8 water quality models when used with Sentinel 2 data. The results showed poor transferability between Landsat and Sentinel 2, with most models experiencing a significant drop in their predictive skill even after recalibration. Sentinel 2 models were then developed for the reservoir based on 153 water quality samples collected over 2 years. The models explored different functional forms, including multiple linear regressions (MLR), multivariate adaptive regression splines (MARS), random forests (RF), and support vector regressions (SVR). The results showed that the RF models outperformed their MLR, MARS, and SVR counterparts with regard to predicting chlorophyll-a, total suspended solids, Secchi disk depth, and phycocyanin. The coefficient of determination (R2) for the RF models varied between 85% for TSS up to 95% for SDD. Moreover, the study explored the potential of quantifying cyanotoxin concentrations indirectly from the Sentinel 2 MSI imagery by benefiting from the strong relationship between cyanotoxin levels and chlorophyll-a concentrations.

Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis.

Freshwater prokaryotic cyanobacteria within harmful algal blooms produce cyanotoxins which are considered major pollutants in the aquatic system. Direct exposure to cyanotoxins through inhalation, skin contact, or ingestion of contaminated drinking water can target the liver and may cause hepatotoxicity. In the current study, we investigated the effect of low concentrations of cyanotoxins on cytotoxicity, inflammation, modulation of unfolded protein response (UPR), steatosis, and fibrosis signaling in human hepatocytes and liver cell models. Exposure to low concentrations of microcystin-LR (MC-LR), microcystin-RR (MC-RR), nodularin (NOD), and cylindrospermopsin (CYN) in human bipotent progenitor cell line HepaRG and hepatocellular carcinoma (HCC) cell lines HepG2 and SK-Hep1 resulted in increased cell toxicity. MC-LR, NOD, and CYN differentially regulated inflammatory signaling, activated UPR signaling and lipogenic gene expression, and induced cellular steatosis and fibrotic signaling in HCC cells. MC-LR, NOD, and CYN also regulated AKT/mTOR signaling and inhibited autophagy. Chronic exposure to MC-LR, NOD, and CYN upregulated the expression of lipogenic and fibrosis biomarkers. Moreover, RNA sequencing (RNA seq) data suggested that exposure of human hepatocytes, HepaRG, and HCC HepG2 cells to MC-LR and CYN modulated expression levels of several genes that regulate non-alcoholic fatty liver disease (NAFLD). Our data suggest that low concentrations of cyanotoxins can cause hepatotoxicity and cell steatosis and promote NAFLD progression.

Effect of cold food storage techniques on the contents of Microcystins and Cylindrospermopsin in leaves of spinach (Spinacia oleracea) and lettuce (Lactuca sativa).

The presence of Cylindrospermopsin (CYN) and Microcystins (MCs) in vegetables is considered as a significant worldwide toxicological risk. Thus, this work aims to assess for the first time the impact of refrigeration (4°C) and freezing (-20°C) on the levels of CYN, MCs and their mixtures (CYN+MCs) in lettuce and spinach. Samples were spiked with 750μg cyanotoxins/g dry weight (d.w.). Several storage conditions were studied: refrigeration after 24, 48h and 7 days, and freezing for 7 days, 1 and 3 months. Cyanotoxin concentrations were determined by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS). For CYN, refrigeration at 48h and 7 days was effective to decrease its concentrations up to 26% and 32%, respectively, in spinach. For MCs, refrigeration was only effective in lettuce compared to spinach, showing an important decrease of 80.3% MC-LR and 85.1% MC-YR. In spinach, CYN was stable after 3 months freezing, whereas MC contents were still reduced up to 44%. Overall, cyanotoxins were less stable in the mixture compared to individual toxins for both processes, and the effect of these storage techniques were toxin and food-specific. Further studies of cyanotoxins in foods are required for evaluating the risk for humans.

A Review of In Situ Methods-Solid Phase Adsorption Toxin Tracking (SPATT) and Polar Organic Chemical Integrative Sampler (POCIS) for the Collection and Concentration of Marine Biotoxins and Pharmaceuticals in Environmental Waters.

Solid Phase Adsorption Toxin Tracking (SPATT) and Polar Organic Chemical Integrative Sampler (POCIS) are in situ methods that have been applied to pre-concentrate a range of marine toxins, pesticides and pharmaceutical compounds that occur at low levels in marine and environmental waters. Recent research has identified the widespread distribution of biotoxins and pharmaceuticals in environmental waters (marine, brackish and freshwater) highlighting the need for the development of effective techniques to generate accurate quantitative water system profiles. In this manuscript, we reviewed in situ methods known as Solid Phase Adsorption Toxin Tracking (SPATT) and Polar Organic Chemical Integrative Sampler (POCIS) for the collection and concentration of marine biotoxins, freshwater cyanotoxins and pharmaceuticals in environmental waters since the 1980s to present. Twelve different adsorption substrates in SPATT and 18 different sorbents in POCIS were reviewed for their ability to absorb a range of lipophilic and hydrophilic marine biotoxins, pharmaceuticals, pesticides, antibiotics and microcystins in marine water, freshwater and wastewater. This review suggests the gaps in reported studies, outlines future research possibilities and guides researchers who wish to work on water contaminates using Solid Phase Adsorption Toxin Tracking (SPATT) and Polar Organic Chemical Integrative Sampler (POCIS) technologies.

Dissolved organic nitrogen, dinoflagellates and cyanobacteria in two eutrophic lakes: Analysis by inferential modelling

The Lake Suwa (Japan) has a history of non-N-fixing Microcystis blooms. Lake Kinneret (Israel) experienced multiannual periods of sole domination by the dinoflagellate Peridinium gatunense and periods dominated seasonally by P. gatunense or cyanobacteria. Extensive studies have been carried out in both lakes regarding the role of dissolved inorganic nitrogen and phosphorus as drivers of primary productivity. There is growing evidence that dissolved organic nitrogen (DON) compounds also influence not only biomass and structure of phytoplankton communities but also microcystin production. This study focuses on relationships of DON with: (1) population dynamics of Microcystis spp. and concentrations of microcystins in Lake Suwa, and (2) population dynamics of P. gatunense as well as N- and non-N-fixing cyanobacteria in Lake Kinneret. Modelling results for historical data of Lake Suwa by means of the hybrid evolutionary algorithm HEA revealed that the prediction of abundances of four Microcystis species and concentrations of cyanotoxins achieved higher coefficients of correlation when DON/DIN-ratios were included as drivers. Population dynamics of P. gatunense in Lake Kinneret appeared to have a strong inverse relationships with DON/DIN-ratios reflected by inferential models of HEA with higher coefficients of correlation when driven by DON/DIN-ratios. When DON/DIN-ratios were included as drivers, models of Microcystis spp. in Lake Kinneret performed higher coefficients of determination compared to models of N-fixing cyanobacteria. The study highlights the need to consider DON for improved understanding and management of population dynamics of cyanobacteria and dinoflagellates in freshwater lakes.

Statistical model applied in the qualitative and quantitative analysis of the relationships between environmental variables and cyanotoxin concentrations in tropical reservoirs.

This article presents the use of generalized additive model for location scale and shape (GAMLSS) in the qualitative and quantitative analysis of the relationships between environmental parameters and cyanotoxins in the tropical reservoirs of Colombia. The physicochemical parameters were evaluated on each of the cyanotoxins detected with levels high by ultra-high-performance liquid chromatography coupled to triple quadrupole mass spectrometry. The models showed a relation between cyanotoxins and the nutrients as soluble total Kjeldahl nitrogen (NTK), soluble phosphorus (soluble P), total phosphorus (total P), and nitrates (NO3-), among other nutrients that are necessary for the growth of organisms that produce toxins. This model confirms that the NTK and soluble P variables favor an increase in the concentration of MC-LR and MC-YR cyanotoxins in the Abreo Malpaso reservoir. The variables NO3- and dissolved O2 contribute to a higher concentration of MC-LR, MC-YR, and MC-ADRR cyanotoxins in the Peñol reservoir. Finally, the variable total P favors the increase in the concentration of MC-LR cyanotoxin and the variable dissolved O2 favors the increase in the concentration of cyanotoxin [D-Asp3, (E)-Dhb7] -MC-RR in the Playas reservoir.

Phytoplankton Communities and Cyanotoxin Production in Some Bulgarian Lowland Lakes and Reservoirs

One of the most evident consequences of eutrophication of waters is the progressive spreading of persistent cyanobacterial blooms. They are often accompanied by the production of cyanotoxins in concentrations, which are hazardous for human health. In this research, we analysed phytoplankton communities in four lowland water bodies, for the presence of cyanobacterial blooms and toxin production. The cyanobacterial biovolumes we found, determine three of the lowland water bodies: Onogur Reservoir (OR), Asparuhov Val Reservoir (AVR), and Srebarna Lake (SL) as “Alert Level 1” of potentially hazardous levels of cyanotoxins. Cyanobacterial biovolume exceeds the threshold value of 8 mm3 L-1 (recreational waters) in AVR and SL at the end of the summer period. In OR, we registered sustainable bloom of Microcystis spp. during the whole summer season, and extremely high average seasonal value of the total biovolume (146.5 mm3 L-1). Micro-cystins were reported in all four analysed water bodies, with the highest concentration in OR (6 µg L-1). Cylindrospermopsin was detected in AVR and OR, while saxitoxins were in AVR and SL. The concentrations of cyanotoxins do not exceed the guideline values in recreational waters. However, the increased biovolumes of cyanobacteria are a signal that in three of the analysed water bodies, monitoring is recommended at the levels of cyanotoxins during the summer period.

Harmful algal blooms and cyanotoxins in Lake Amatitlán, Guatemala, coincided with ancient Maya occupation in the watershed

Human-induced deforestation and soil erosion were environmental stressors for the ancient Maya of Mesoamerica. Furthermore, intense, periodic droughts during the Terminal Classic Period, ca. Common Era 830 to 950, have been documented from lake sediment cores and speleothems. Today, lakes worldwide that are surrounded by dense human settlement and intense riparian land use often develop algae/cyanobacteria blooms that can compromise water quality by depleting oxygen and producing toxins. Such environmental impacts have rarely been explored in the context of ancient Maya settlement. We measured nutrients, biomarkers for cyanobacteria, and the cyanotoxin microcystin in a sediment core from Lake Amatitlán, highland Guatemala, which spans the last ∼2,100 y. The lake is currently hypereutrophic and characterized by high cyanotoxin concentrations from persistent blooms of the cyanobacterium Microcystis aeruginosa Our paleolimnological data show that harmful cyanobacteria blooms and cyanotoxin production occurred during periods of ancient Maya occupation. Highest prehistoric concentrations of cyanotoxins in the sediment coincided with alterations of the water system in the Maya city of Kaminaljuyú, and changes in nutrient stoichiometry and maximum cyanobacteria abundance were coeval with times of greatest ancient human populations in the watershed. These prehistoric episodes of cyanobacteria proliferation and cyanotoxin production rivaled modern conditions in the lake, with respect to both bloom magnitude and toxicity. This suggests that pre-Columbian Maya occupation of the Lake Amatitlán watershed negatively impacted water potability. Prehistoric cultural eutrophication indicates that human-driven nutrient enrichment of water bodies is not an exclusively modern phenomenon and may well have been a stressor for the ancient Maya.

Oxidation of Cylindrospermopsin by Fenton Process: A Bench-Scale Study of the Effects of Dose and Ratio of H2O2 and Fe(II) and Kinetics.

The cyanotoxin cylindrospermopsin (CYN) has become a significant environmental and human health concern due to its high toxicological potential and widespread distribution. High concentrations of cyanotoxins may be produced during cyanobacterial blooms. Special attention is required when these blooms occur in sources of water intended for human consumption since extracellular cyanotoxins are not effectively removed by conventional water treatments, leading to the need for advanced water treatment technologies such as the Fenton process to produce safe water. Thus, the present study aimed to investigate the application of the Fenton process for the degradation of CYN at bench-scale. The oxidation of CYN was evaluated by Fenton reaction at H2O2/Fe(II) molar ratio in a range of 0.4 to 4.0, with the highest degradation of about 81% at molar ratio of 0.4. Doubling the concentrations of reactants for the optimized H2O2/Fe(II) molar ratio, the CYN degradation efficiency reached 91%. Under the conditions studied, CYN degradation by the Fenton process followed a pseudo-first-order kinetic model with an apparent constant rate ranging from 0.813 × 10−3 to 1.879 × 10−3 s−1.

Foreseen Effects of Climate-Impacted Scenarios on the Photochemical Fate of Selected Cyanotoxins in Surface Freshwaters.

Cyanobacteria populate most water environments, and their ability to effectively exploit light and nutrients provide them with a competitive advantage over other life forms. In particular conditions, cyanobacteria may experience considerable growth and give rise to the so-called harmful algal blooms (HABs). HABs are often characterized by the production of cyanotoxins, which cause adverse effects to both aquatic organisms and humans and even threaten drinking water supplies. The concentration of cyanotoxins in surface waters results from the budget between production by cyanobacteria and transformation, including photodegradation under sunlight exposure. Climate change will likely provide favorable conditions for HABs, which are expected to increase in frequency over both space and time. Moreover, climate change could modify the ability of some surface waters to induce phototransformation reactions. Photochemical modeling is here carried out for two cyanotoxins of known photoreaction kinetics (microcystin-LR and cylindrospermopsin), which follow different phototransformation pathways and for particular freshwater scenarios (summertime stratification in lakes, water browning, and evaporative water concentration). On this basis, it is possible to quantitatively predict that the expected changes in water-column conditions under a changing climate would enhance photodegradation of those cyanotoxins that are significantly transformed by reaction with the triplet states of chromophoric dissolved organic matter (3CDOM*). This is known to be the case for microcystin-LR, for which faster photodegradation in some environments would at least partially offset enhanced occurrence. Unfortunately, very few data are currently available for the role of 3CDOM* in the degradation of other cyanotoxins, which is a major knowledge gap in understanding the link between cyanotoxin photodegradation and changing climate.

Co-occurring microorganisms regulate the succession of cyanobacterial harmful algal blooms

Cyanobacterial harmful algal blooms (CyanoHABs) have been found to transmit from N2 fixer-dominated to non-N2 fixer-dominated in many freshwater environments when the supply of N decreases. To elucidate the mechanisms underlying such “counter-intuitive” CyanoHAB species succession, metatranscriptomes (biotic data) and water quality-related variables (abiotic data) were analyzed weekly during a bloom season in Harsha Lake, a multipurpose lake that serves as a drinking water source and recreational ground. Our results showed that CyanoHABs in Harsha Lake started with N2-fixing Anabaena in June (ANA stage) when N was high, and transitioned to non-N2-fixing Microcystis- and Planktothrix-dominated in July (MIC-PLA stage) when N became limited (low TN/TP). Meanwhile, the concentrations of cyanotoxins, i.e., microcystins were significantly higher in the MIC-PLA stage. Water quality results revealed that N species (i.e., TN, TN/TP) and water temperature were significantly correlated with cyanobacterial biomass. Expression levels of several C- and N-processing-related cyanobacterial genes were highly predictive of the biomass of their species. More importantly, the biomasses of Microcystis and Planktothrix were also significantly associated with expressions of microbial genes (mostly from heterotrophic bacteria) related to processing organic substrates (alkaline phosphatase, peptidase, carbohydrate-active enzymes) and cyanophage genes. Collectively, our results suggest that besides environmental conditions and inherent traits of specific cyanobacterial species, the development and succession of CyanoHABs are regulated by co-occurring microorganisms. Specifically, the co-occurring microorganisms can alleviate the nutrient limitation of cyanobacteria by remineralizing organic compounds.