Current-clamp Research Articles

A selective chromo-fluorogenic chemosensor for visual detection of solution and vapor phase of sarin, tabun mimics diethyl chlorophosphate, diethyl cyanophosphonate

The abuse of nerve agents has become a severe threat to peaceful modern civilization. Hence, it is of paramount importance to the scientific communities to detect and degrade them for a modern society. In the present contribution, we have introduced a selective chromo-fluorogenic probe (AMP) based on neutral red dye for visual detection of the solution and vapor phase of sarin & tabun mimics, namely diethyl chlorophosphate (DCP) and diethyl cyanophosphonate (DCNP), respectively. A ratiometric UV–visible absorption spectral change with colorimetric visualization from yellow to bright pink is observed in the presence of both mimics. A “turn-off” photoluminescence from yellow color to non-fluorescence is observed in the presence of both mimics. The spectral and chromo-fluorgenic behavior is reversed in the presence of triethylamine (TEA). The sensitivity of AMP towards the detection of targeted analytes is recorded in the μM range. Additionally, filter paper-based strips are fabricated for the actual applicability of the current probe to identify trace amounts of both analytes in the presence of various types of analogous target analytes. The vapor phase sensing and two types of soil analysis are also employed to check the sensor's potential in real-life applications. The present report has revealed prompt, sensitive, selective, in-situ, accurate sample detectors of sarin & tabun gas in actual threatening situations.

Analytical model of eddy current bobbin coil probe responses at support plates in steam generator and heat exchanger tubes

The steam generators (SGs) of CANadian Deuterium Uranium nuclear reactors require periodic inspections to ensure their safe operation. Eddy current (EC) testing is the primary method by which the SG tubes are inspected. Many conditions in the SG tubes affect the EC response, such as fretting, pitting, cracking, as well as tube expansion, and the presence of tubesheet and support structures. When two or more of these parameters overlap, the EC signals from degradation induced wall losses are frequently difficult to interpret because these wall loss signals are often distorted due to interference with background signal variations. In this paper, a novel analytical model that describes the impedance of a bobbin coil with a plate encircling an arbitrary number of cylindrical conductors is developed. The model is validated against finite element method modelling and experiment. This model can be used to simulate the main probe response, while inspecting SG tubes near the tubesheet and support structures and thereby, provides the potential to separate out smaller flaw responses.

A Small-Molecule Ratiometric Photoacoustic Probe for the High-Spatiotemporal-Resolution Imaging of Copper(II) Dynamics in the Mouse Brain.

Copper dysmetabolism is associated with various neurodegenerative disorders, making high-spatiotemporal-resolution imaging of Cu2+ in the brain essential for understanding the underlying pathophysiological processes. Nevertheless, the current probes encounter obstacles in crossing the blood-brain barrier (BBB) and providing high-spatial-resolution in deep tissues. Herein, we present a photoacoustic probe capable of imaging Cu2+ dynamics in the mouse brain with high-spatiotemporal-resolution. The probe demonstrates selective ratiometric and reversible responses to Cu2+ , while also efficiently crossing the BBB. Using the probe as the imaging agent, we successfully visualized Cu2+ in the brain of Parkinson's disease (PD) model mouse with a remarkable micron-level resolution. The imaging results revealed a significant increase in Cu2+ levels in the cerebral cortex as PD progresses, highlighting the close association between Cu2+ alternations in the region and the disease. We also demonstrated that the probe can be used to monitor changes in Cu2+ distribution in the PD model mouse brain during L-dopa intervention. Mechanism studies suggest that the copper dyshomeostasis in the PD mouse brain was dominated by the expression levels of divalent metal transporter 1. The application of our probe in imaging Cu2+ dynamics in the mouse brain offers valuable insights into the copper-related molecular mechanisms underlying neurodegenerative diseases.

A Small‐Molecule Ratiometric Photoacoustic Probe for the High‐Spatiotemporal‐Resolution Imaging of Copper(II) Dynamics in the Mouse Brain

AbstractCopper dysmetabolism is associated with various neurodegenerative disorders, making high‐spatiotemporal‐resolution imaging of Cu2+ in the brain essential for understanding the underlying pathophysiological processes. Nevertheless, the current probes encounter obstacles in crossing the blood–brain barrier (BBB) and providing high‐spatial‐resolution in deep tissues. Herein, we present a photoacoustic probe capable of imaging Cu2+ dynamics in the mouse brain with high‐spatiotemporal‐resolution. The probe demonstrates selective ratiometric and reversible responses to Cu2+, while also efficiently crossing the BBB. Using the probe as the imaging agent, we successfully visualized Cu2+ in the brain of Parkinson's disease (PD) model mouse with a remarkable micron‐level resolution. The imaging results revealed a significant increase in Cu2+ levels in the cerebral cortex as PD progresses, highlighting the close association between Cu2+ alternations in the region and the disease. We also demonstrated that the probe can be used to monitor changes in Cu2+ distribution in the PD model mouse brain during L‐dopa intervention. Mechanism studies suggest that the copper dyshomeostasis in the PD mouse brain was dominated by the expression levels of divalent metal transporter 1. The application of our probe in imaging Cu2+ dynamics in the mouse brain offers valuable insights into the copper‐related molecular mechanisms underlying neurodegenerative diseases.

Nano-positive up negative down in binary oxide ferroelectrics

Ferroelectric HfO2 and ZrO2-based materials are promising candidates for next-generation ferroelectric devices, but their characterization is challenging due to complex factors such as crystal phases, degradation mechanisms, and local inhomogeneities. In this work, we propose a novel nanosized positive-up-negative-down (PUND) method to assess the ferroelectric response of doped-HfO2. This method is based on actual current probing and is immune to most electrostatic artifacts that plague other electrical atomic force microscopy (AFM) techniques. We demonstrate the PUND method’s ability to induce ferroelectric switching in Si-doped HfO2 and investigate the distinctive PUND response obtained for individual grains. We also extract the polarization charge based on a direct estimate of the tip–sample contact area. The proposed PUND method is a significant innovation as it is a method to combine the high spatial resolution of AFM with the immunity to electrostatic artifacts of current probing. This makes it a powerful tool for studying ferroelectric materials at the nanoscale, where local inhomogeneities and other effects can have a significant impact on their behavior. The PUND method is also notable for its ability to extract polarization charge based on a direct estimate of the tip–sample contact area. This is a significant improvement over previous methods, which often relied on indirect or approximate estimates of the contact area. Overall, the PUND method is a novel and innovative technique that offers significant advantages for the characterization of ferroelectric materials at the nanoscale. It is expected to have a major impact on the research and development of next-generation ferroelectric devices.

Advancing eddy current detection probes with magnetoelectric composite Materials: Enhancing crack detection in conductive materials

Eddy current testing (ECT) is a non-destructive method widely employed for detecting cracks in various conductive materials. However, the applicability of detection probes in low-frequency settings is limited by their sensitivity and size. This study introduces a novel magnetic detection probe for ECT, utilizing dual-magnetoelectric (ME) composites in a differential noise cancellation mode to enhance detection capabilities at low frequencies (kHz). Our investigation demonstrates the superior performance of the proposed detection probe compared to traditional copper coils. Through numerical analysis of the ME detection probe's voltage output, we establish its efficacy in identifying small defects or cracks in conductive materials. Furthermore, this research explores a crucial factor for accurately estimating crack length and depth in system analysis applications. The study significantly contributes to the field of magnetism and magnetic materials, paving the way for the development of more effective and versatile crack detection techniques.

Characteristics of an AC rotating gliding arc discharge in NH3 and air atmospheres

Plasmas have emerged as a promising technology for the utilization of NH3 as a carbon-free fuel for direct plasmas-assisted combustion and hydrogen production. This study aims to explore the electrical and optical emission characteristics of the rotating gliding arc (RGA) discharge in NH3 and air swirling flows over a wide range of nominal power inputs. The electrical characteristics were measured using voltage–current probes, while a spectrometer was employed to assess the spectral characteristics. Additionally, a synchronized high-speed camera equipped with a dual-scope objective was utilized to capture transient phenomena of the arc in the optical emission spectrum. Both the spark-type and glow-type discharges were observed in RGA. Interestingly, the spark-type discharge frequency exhibited a non-monotonic variation with increasing nominal power inputs for both NH3 and air, while the glow-type discharge frequency displayed a monotonous upward trend. Regarding the spectral emission characteristics in NH3, a dramatic transition of the arc emission spectrum from Hα to NH2* was observed as nominal power inputs increased. The Hα and NH2* emissions that are dominated in the spark-type and glow-type discharges, respectively, and the intensity ratio of Hα and NH2* emission shows evident correlations with discharge current and electric field strength. In summary, this study represents the first investigation into the electrical and spectral characteristics of RGA in NH3.

Point projection radiography of electromagnetically accelerated flyer plates with an external X-pinch driver.

A platform for flyer plate benchmarking experiments has been developed, with an external X-pinch driver for point projection radiography. The experiments were performed using CEPAGE, a low inductance pulsed power machine at First Light Fusion (2 MA, 1.4 µs), with a new vacuum transmission line and flyer load hardware designed specifically to give a line of sight for radiography. A broadband 10-20keV x-ray source was produced by a portable X-pinch driver (140kA, 350ns) [Strucka etal., Matter Radiat. Extremes 7, 016901 (2021)] and was used to image the flyer. Radiography compliments the pre-existing diagnostic suite, which consists of current probes, velocimetry, and side-on optical probing of the impact shock transmitted into a transparent sample. The platform allows for significant insights into the 2D and 3D nature of the flyer launch, such as deformation and instability formation. It was used to diagnose a 10 × 9 × 1mm3 aluminum flyer, which reached a peak velocity of 4.2 km s-1 before impact with a poly(methylmethacrylate) sample. The experimental configuration, on-shot source characterization, and the results from two flyer plate experiments on CEPAGE are discussed.

A MODEL OF THE MAGNETIC FIELD OF A CRACK SURROUNDED BY A UNIFORM EDDY CURRENT

Eddy current probes with uniform eddy currents, which are increasingly often used in practice, use the measurement of various components of the magnetic field of a defect arising when the current flows around surface cracks. The patterns of formation of components of the magnetic field strength of the defect that are orthogonal to the surface of the tested object (normal) Hn, as well as parallel to the plane of the crack (tangential) Ht are quite obvious and allow us to establish that their extreme values arise in the plane of the crack above its ends and center, respectively. At the same time, there does not exist a satisfactory model of the mechanism of formation of the axial component Hx, directed perpendicular to the crack plane. Both experiments and numerical calculations show the complex character of the function Hx. It has 4 regions with different directions in the vicinity of the crack, and its four extrema arise at a certain distance from the plane of the crack and are shifted relative to the axis of symmetry. The lack of a simple model of the formation of the function Hx makes it difficult to understand the functioning and develop appropriate eddy current transducers. The present paper addresses this problem.

Quantum Dot Fluorescent Imaging: Using Atomic Structure Correlation Studies to Improve Photophysical Properties.

Efforts to study intricate, higher-order cellular functions have called for fluorescence imaging under physiologically relevant conditions such as tissue systems in simulated native buffers. This endeavor has presented novel challenges for fluorescent probes initially designed for use in simple buffers and monolayer cell culture. Among current fluorescent probes, semiconductor nanocrystals, or quantum dots (QDs), offer superior photophysical properties that are the products of their nanoscale architectures and chemical formulations. While their high brightness and photostability are ideal for these biological environments, even state of the art QDs can struggle under certain physiological conditions. A recent method correlating electron microscopy ultrastructure with single-QD fluorescence has begun to highlight subtle structural defects in QDs once believed to have no significant impact on photoluminescence (PL). Specific defects, such as exposed core facets, have been shown to quench QD PL in physiologically accurate conditions. For QD-based imaging in complex cellular systems to be fully realized, mechanistic insight and structural optimization of size and PL should be established. Insight from single QD resolution atomic structure and photophysical correlative studies provides a direct course to synthetically tune QDs to match these challenging environments.

Effects of kappa-opioid agonist U-50488 and p38 MAPK inhibitor SB203580 on the spike activity of pyramidal neurons in the basolateral amygdala

Introduction: Kappa-opioid receptor (KOR) signaling in the basolateral amygdala (BLA) underlies KOR agonist-induced aversion. In this study, we aimed to understand the individual and combined effects of KOR agonist U-50488 and p38 MAPK inhibitor SB203580 on the spiking activity of pyramidal neurons in the BLA to shed light on the complex interplay between KORs, the p38 MAPK, and neuronal excitability. Materials and Methods: Electrophysiological experiments were performed using the patch-clamp technique in the whole-cell configuration. Rat brain slices containing the amygdala were prepared, and pyramidal neurons within the BLA were visually patched and recorded in the current clamp mode. The neurons were identified by their accommodation properties and neural activity signals were amplified and analyzed. Using local perfusion, we obtained three dose-response curves for: (a) U-50488 (0.001–10 μM); (b) U-50488 (0.001–10 μM) in the presence of SB203580 (1 μM); and (c) U-50488 (0.01–10 μM) in the presence of SB203580 (5 μM). Results: After the application of U-50488, pyramidal neurons had a higher action potential firing rate in response to a current injection than control neurons (p<0.001). The dose-dependent curves we obtained indicate that the combination of U-50488 and SB203580 results in non-competitive antagonism. This conclusion is supported by the observed change in the curve’s slope with reduction in the maximum effect of U-50488. Thus, it can be assumed that the increase in spike activity of pyramidal neurons of the amygdala is mediated through the beta-arrestin pathway. When this pathway is blocked, the spike activity reverts to its baseline level. Conclusion: Our study found that the KOR agonist-induced spiking activity of the BLA pyramidal neurons is mediated by the beta-arrestin pathway and can be suppressed by the application of the p38 MAPK inhibitor SB203580.

ONOO- Activatable Fluorescent Sulfur Dioxide Donor for a More Accurate Assessment of Cell Ferroptosis.

Ferroptosis is critical in the treatment of tumor therapies. Thus, monitoring reactive oxygen species (ROS) is of great significance for accurate assessment in ferroptosis without any interference. However, current probes for monitoring ROS during ferroptosis suffer from a drawback in that the probes consume ROS during detection, which inhibits the ferroptosis process and thus affects the accuracy and effectiveness of monitoring the process of ferroptosis. Herein, a new fluorescent donor probe, TFMU-SO2D, with the combination of the moiety of the SO2 donor is designed and synthesized by introducing the aryl boronate moieties that could give it the ability to effectively recognize ONOO-. The released SO2 could consume excess glutathione and regulate oxidative stress by elevating ROS levels, which would offset the ROS depletion by TFMU-SO2D and ensure accuracy in monitoring the ferroptosis process. The experimental results demonstrated that TFMU-SO2D possessed satisfactory performance for monitoring ONOO- as well as simultaneously releasing SO2 in oxidative stress stimulated by monensin and ferroptosis stimulated by erastin and RSL3. Additionally, the capability of SO2 synergized with ferroptosis to inhibit the viability of cancer cells was demonstrated by the CCK8 assay, which may be due to the fact that SO2 can potentiate ferroptosis cell death by increasing the ROS level. Overall, these combined results indicated that TFMU-SO2D possesses the excellent ability to precisely monitor ONOO- during ferroptosis without interference, which is significant for accurately accessing ferroptosis, cancer treatment, and drug development.

Fastener hole inspection using 2D phased array

Fastener hole cracks, resulting from stress concentration around the hole, are potential sources of failure in mechanical structures. Traditionally, inspection involves manually moving a single ultrasonic probe around the hole or using an eddy current probe to move through the hole. However, single ultrasonic probe inspection is time-consuming and needs skilful operators. Eddy current inspection requires the removal of the fastener in advance and does not allow easy crack sizing and visualization. This paper proposes a novel approach that combines a 2D phased array with a coupling block to enable complete fastener hole inspection from a single array position. A single full matrix capture (FMC) data set is collected, from which 3D volumetric images are formed. To obtain clear images of the geometry and potential defects, half-skip and multi-skip total focusing method imaging algorithms are investigated together with transmitter–receiver pairs selection methods to detect different target features. Experimental results demonstrate that the proposed inspection approach can eliminate dead zone problems and capture all target information from a single inspection position. These methods show great potential in enhancing the efficiency and reliability of fastener hole inspections.

Separation of common and differential mode conducted emission: Power combiner/splitters

A conducted emission measurement contains a common and a differential mode component. Accurate separation of these two components is critical, when designing the input filter of a switch mode power supply. Many techniques exists for performing such separation. Some authors suggested the use of wideband transformers, while other prefer current probes. In this paper the use of commercial power splitters/combiners as noise separators are considered. The performance of the noise separators are analyzed and validated based on scattering parameters (S-parameters). Impedance and rejection ratios (common and differential mode) are shown. The results based on S-parameters are used to propose a complete noise separator design. This separator is verified through experimental measurements. Finally an example on how to use the separator, when measuring conducted noise is given.

High resolution magnetic field imaging probe with two rows of TMR sensors for small defects inspection

It is still a challenging problem to detect small defects for eddy current array probes, which requires the probes to possess excellent sensitivity, as well as high spatial resolution. This paper presents a novel high-resolution magnetic field imaging probe with two rows of tunneling magnetoresistance (TMR) array sensors. The bare die sensors are integrated on a printed circuit board by golden wire bonding technology. The two rows of sensors are placed staggered with each other. The data of the two arrays are merged into a matrix, in which way the image pixel pitch is increased to 0.25 mm. The probe employs a differential scheme to suppress the noise, so as to detect the weak signal of small defects. To highlight the weak defect indications, feature extraction and segmentation algorithms are developed. The experimental results confirm that the proposed method can inspect a small defect with dimensions 1 mm (length) × 0.1 mm (width) × 0.1 mm (depth) on a stainless-steel sample.

Electromagnetic Susceptibility Analysis of the Operational Amplifier to Conducted EMI Injected through the Power Supply Port.

Operational amplifiers (op-amps) are widely used in circuit systems. The increasing complexity of the power supply network has led to the susceptibility of the power supply port to electromagnetic interference (EMI) in circuit systems. Therefore, it is necessary to investigate the electromagnetic susceptibility (EMS) of op-amps at the power supply port. In this paper, we assessed the effect of EMI on the operational performance of op-amps through the power supply port by a bulk current injection (BCI) method. Firstly, we conducted the continuous sine wave into the power supply port by a current injection probe and measured the change in the offset voltage under EMI. Secondly, we proposed a new method of conducted susceptibility and obtained the susceptibility threshold regularities of the op-amps at the power supply port under the interference of different waveform signals. Our study provided conclusive evidence that EMI reduced the reliability of the op-amp by affecting the offset voltage of op-amps and demonstrated that the sensitivity type of op-amps was peak-sensitive at the power supply port. This study contributed to a deep understanding of the EMS mechanism and guided the design of electromagnetic compatibility (EMC) of op-amps.

High-Contrast Photoacoustic Imaging of Localized Cysteine in Orthotopic Breast Cancer Enabled by A Totally-Caged Methylene Blue Probe.

High-contrast photoacoustic sensing imaging (PASI) was greatly determined by optical absorption changes of the absorbers usually enabled by activatable probes via controllably converting the absorbed electromagnetic energy to ultrasound waves. However, most of current photoacoustic probes still suffer from limited imaging contrast towards specific species because of their small absorption spectral changes in the near infrared (NIR) region. Herein, we developed a methylene blue-based photoacoustic probe with its NIR optical absorption totally caged, which could afford dramatical "OFF-to-ON" absorption transition for high-contrast photoacoustic imaging towards the localized cysteine. The rationally designed methylene blue-based probe for cysteine (MB-Cys) would keep in off state with almost no absorption in NIR region, while upon activated by cysteine through cyclization reaction with acrylates, it would reconstruct the π-conjugation system to release the free methylene blue with strong absorption centered at 665 nm (>130-fold enhancement). The unique responsive behavior could enable the PASI for photoacoustic mapping the cysteine in orthotopic breast cancer in a high-contrast manner. Therefore, this work established an up-to-date strategy to originally eliminate the background photoacoustic signal for PASI to accurately monitor cysteine in vivo.

MiR-133a-3p/TRPM4 axis improves palmitic acid induced vascular endothelial injury.

Background: Vascular endothelial injury is a contributing factor to the development of atherosclerosis and the resulting cardiovascular diseases. One particular factor involved in endothelial cell apoptosis and atherosclerosis is palmitic acid (PA), which is a long-chain saturated fatty acid. In addition, transient receptor potential melastatin 4 (TRPM4), a non-selective cation channel, plays a significant role in endothelial dysfunction caused by various factors related to cardiovascular diseases. Despite this, the specific role and mechanisms of TRPM4 in atherosclerosis have not been fully understood. Methods: The protein and mRNA expressions of TRPM4, apoptosis - and inflammation-related factors were measured after PA treatment. The effect of TRPM4 knockout on the protein and mRNA expression of apoptosis and inflammation-related factors was detected. The changes of intracellular Ca2+, mitochondrial membrane potential, and reactive oxygen species were detected by Fluo-4 AM, JC-1, and DCFH-DA probes, respectively. To confirm the binding of miR-133a-3p to TRPM4, a dual luciferase reporter gene assay was conducted. Finally, the effects of miR-133a-3p and TRPM4 on intracellular Ca2+, mitochondrial membrane potential, and reactive oxygen species were examined. Results: Following PA treatment, the expression of TRPM4 increases, leading to calcium overload in endothelial cells. This calcium influx causes the assemblage of Bcl-2, resulting in the opening of mitochondrial calcium channels and mitochondrial damage, ultimately triggering apoptosis. Throughout this process, the mRNA and protein levels of IL-1β, ICAM-1, and VCAM1 significantly increase. Database screenings and luciferase assays have shown that miR-133a-3p preferentially binds to the 3'UTR region of TRPM4 mRNA, suppressing TRPM4 expression. During PA-induced endothelial injury, miR-133a-3p is significantly decreased, but overexpression of miR-133a-3p can attenuate the progression of endothelial injury. On the other hand, overexpression of TRPM4 counteracts the aforementioned changes. Conclusion: TRPM4 participates in vascular endothelial injury caused by PA. Therefore, targeting TRPM4 or miR-133a-3p may offer a novel pharmacological approach to preventing endothelial injury.

Non-Contact Current Measurement for Three-Phase Rectangular Busbars Using TMR Sensors.

This paper proposes a non-contact current measurement method for three-phase rectangular busbars based on TMR (tunneling magneto-resistance) sensors, due to their advantages of large dynamic range, wide bandwidth, light weight, and easy installation. A non-contact current sensor composed of only three TMR sensors is developed and the TMR sensors are respectively placed at a location with a certain distance from the surface of each rectangular busbar to measure the magnetic fields generated by the busbar currents. To calibrate the developed current sensor, i.e., to establish the relationship between the magnetic fields measured by the TMR sensors and the currents flowing in the three-phase rectangular busbars, we designed a thyristor-controlled resistive load as a calibrator, which is connected to a downstream branch of the distribution cabinet. By switching the resistive load, a calibration current, which can be identified from the background current, is generated in one rectangular busbar and its value is measured at the location of the calibrator, and transmitted wirelessly to the location of the TMR sensors. A new and robust method is proposed to extract the voltage components, corresponding to the calibration current, from the voltage waveforms of the TMR sensors. By calculating the proportional coefficients between the calibration currents and the extracted voltage components, online calibration of the current sensor is achieved. We designed and implemented a current measurement system consisting of a current sensor using TMR sensors, a thyristor-controlled resistive load for current sensor calibration, and a data acquisition circuit based on a multi-channel analog-to-digital converter (ADC). Current measurement experiments were performed in a practical distribution cabinet installed in our laboratory. Compared to the measurement results using a commercial current probe with an accuracy of 1%, the relative error of the measured currents in RMS is less than 2.5% and the phase error is less than 1°, while the nonlinearity error of the current sensor is better than 0.8%.

Mild synthesis of ultra-bright carbon dots with solvatochromism for rapid lipid droplet monitoring in varied physiological processes.

Lipid droplets (LDs) participating in various cellular activities and are increasingly being emphasized. Fluorescence imaging provides powerful tool for dynamic tracking of LDs, however, most current LDs probes remain inconsistent performance such as low Photoluminescence Quantum Yield (PLQY), poor photostability and tedious washing procedures. Herein, a novel yellow-emissive carbon dot (OT-CD) has been synthesized conveniently with high PLQY up to 90%. Besides, OT-CD exhibits remarkable amphiphilicity and solvatochromic property with lipid-water partition coefficient higher than 2, which is much higher than most LDs probes. These characters enable OT-CD high brightness, stable and wash-free LDs probing, and feasible for in vivo imaging. Then, detailed observation of LDs morphological and polarity variation dynamically in different cellular states were recorded, including ferroptosis and other diseases processes. Furthermore, fast whole imaging of zebrafish and identified LD enrichment in injured liver indicate its further feasibility for in vivo application. In contrast to the reported studies to date, this approach provides a versatile conventional synthesis system for high-performance LDs targeting probes, combing the advantages of easy and high-yield production, as well as robust brightness and stability for long-term imaging, facilitating investigations into organelle interactions and LD-associated diseases.