Curative Effect Of Patients Research Articles

Efficacy of miR-122 Nanoparticle Drug Delivery System on the Patients with Drug-Induced Liver Injury

In order to explore the effect of MiR-122 nanoparticle (NP) drug delivery system (NPDDS) on the condition and curative effect of patients with drug-induced liver injury (DILI). The phosphate buffered saline solution was added to the solution of Monoammonium Glycyrrhizanate (MAG) NPs to make the suspension. Then, 50 μL of the MAG β-cyclodextrin star-shaped cationic polymer solution was added to the 100 μg/mL MiR-122 solution and form MAG/MiR-122 co-loaded NPs. 40 cases of acute DILI patients in hospital were selected as the research subjects. Control group, polymer group, drug-loaded group, and co-loaded group were set up, and fluorescence quantitative PCR was employed to measure the expression level of MiR-122 in the cells. The untreated DILI cells were set as control group, while the MAG/MiR-122 co-loaded NPs-treated DILI cells were set as treatment group. The treatment effects of the two groups were analyzed and compared. The results showed that the MAG/MiR co-loaded NPs prepared were of regular round shape and uniform particle size distribution. The expression of MiR-122 was low in control group, and its content in injured cells was extremely low. The expression of MiR-122 in the co-loaded group was superior to drug-loaded group. The effective rate of drug treatments in treatment group was significantly superior to control group, and the differences were substantial (P < 0.05). The number of red blood cells (RBC) of treatment group decreased notably relative to controls, while differences on white blood cells (WBC) and platelets (PLT) between two groups were not considerable (P > 0.05). In conclusion, MAG/MiR co-loaded NPs can effectively improve the therapeutic effect of DILI. Moreover, early DILI screening can be implemented by detecting the expression level of MiR-122 in the cells of DILI patients.

Influence of Standardized Nursing Intervention Combined with Mindfulness Stress Reduction Training on the Curative Effect, Negative Emotion, and Quality of Life in Patients with Chronic Gastritis and Gastric Ulcer.

Objective To explore the curative effect of standardized nursing intervention combined with mindfulness stress reduction training in patients with chronic gastritis and gastric ulcer and its influence on negative emotion and quality of life. Methods Total of 146 patients with chronic gastritis and gastric ulcer admitted to our hospital from August 2018 to February 2020 were collected and divided into the control group (n = 73) and study group (n = 73) according to patients' wishes and the random number method. The control group received traditional nursing measures. The study group received standardized nursing intervention combined with mindfulness stress reduction training. The general data, nursing efficiency, negative emotions, self-efficacy, quality of life, and nursing satisfaction of the two groups were recorded. Results The nursing effective rate of the study group (91.78%) was higher than the control group (79.45%) (P < 0.05). After intervention, the self-rating anxiety scale score and self-rating depressive scale score of the study group were lower than the control group (P < 0.05). After intervention, the general self-efficacy scale score and the generic quality of life inventory-74 scores of the study group were higher than the control group (P < 0.05). The nursing satisfaction rate of the study group (89.04%) was higher than the control group (75.34%) (P < 0.05). Conclusion Standardized nursing intervention combined with mindfulness stress reduction training has a good curative effect in patients with chronic gastritis and gastric ulcer, which is beneficial to reduce negative emotions, increase self-efficacy, improve quality of life, and improve nursing satisfaction.

Effect of Root Canal Therapy Combined with Full Crown Restoration on the Level of Inflammatory Factors and Chewing Function in Patients with Cracked Teeth and Chronic Pulpitis.

Objective To explore the clinical effect of root canal therapy combined with full crown restoration in patients with cracked teeth and chronic pulpitis. Methods From May 2018 to June 2020, 87 patients with cracked teeth and chronic pulpitis in our hospital were selected; the patients were randomly divided into the control group and the research group by random number method. The control group only used root canal therapy; the research group used root canal therapy combined with full crown restoration. The therapeutic effect, levels of inflammatory factors, chewing function, periodontal index, complications, and quality of life were compared between the two groups. Results The total effective rate of the research group (97.78%) was better than the total effective rate of the control group (85.71%) (P < 0.05). Compared with before treatment, the serum levels of interleukin-1β (IL-1β), IL-6, and C-reactive protein (CRP) of the two groups of patients decreased after treatment. After treatment, compared with the control group, the serum levels of IL-1β, IL-6, and CRP in the research group decreased (P < 0.05). Compared with before treatment, the bite force of teeth and chewing efficiency of the two groups of patients increased after treatment. After treatment, compared with the control group, the bite force of teeth and chewing efficiency of the research group increased (P < 0.05). Compared with before treatment, the plaque index (PLI), probing depth (PD), gingival sulcus bleeding index (BI), and gingival index (GI) of the two groups of patients decreased after treatment. After treatment, compared with the control group, the PLI, PD, BI, and GI of the research group decreased (P < 0.05). The total incidence of complications in the research group was (11.11%), and the total incidence of complications in the control group was (16.67%); there was no significant difference between the two groups (P > 0.05). After treatment, compared with the control group, the quality of life scores of the patients in the research group were reduced (P < 0.05). Conclusion Root canal therapy and full crown restoration have a definite curative effect in patients with cracked teeth and chronic pulpitis, which can improve the inflammatory response, restore chewing function, maintain periodontal health, improve the quality of life, and do not increase the incidence of complications, so it has good application value.

Epigallocatechin-3 gallate regulates macrophage subtypes and immunometabolism to ameliorate experimental autoimmune encephalomyelitis

Epigallocatechin-3 gallate (EGCG) is a polyphenolic component of tea and has potential curative effects in patients with autoimmune diseases. Multiple sclerosis (MS) is an autoimmune disease affecting the central nervous system (CNS). It remains unknown whether EGCG can regulate macrophage subtypes in MS. Here we evaluated the effects of EGCG in experimental autoimmune encephalomyelitis (EAE), MS mouse model. We found that EGCG treatment reduced EAE severity and macrophage inflammation in the CNS. Moreover, EAE severity was well correlated with the ratio of M1 to M2 macrophages, and EGCG treatment suppressed M1 macrophage-mediated inflammation in spleen. In vitro experiments showed that EGCG inhibited M1 macrophage polarization, but promoted M2 macrophage polarization. These effects were likely to be related to the inhibition of nuclear factor-κB signaling and glycolysis in macrophages by EGCG in macrophages. Overall, these findings provided important insights into the mechanisms through which EGCG may mediate MS.

Intelligent Three-Dimensional Reconstruction Algorithm-Based X-Ray Film for Analysis of the Changes in Sagittal Parameters and Curative Effect of Anterior Cervical Surgery

In this paper, the change characteristics of sagittal parameters of the patient’s body before and after anterior cervical surgery and rehabilitation effect were analyzed. The rotation transformation and perspective projection transformation were applied to construct a positive and lateral position (PLP) matching-based three-dimensional reconstruction (PLP-3DR) algorithm, which was compared with the marching cubes (MC) algorithm and gravity point (GP) algorithm. PLP-3DR was adopted in X-ray image diagnosis of 124 cervical spondylosis patients. Results showed that sensitivity, specificity, and accuracy of PLP-3DR were markedly higher than those of MC and GP ( P &lt; 0.05 ). The postoperative C2-7 Cobbs’s angle (C2-7Cobb) (11.27 ± 8.41°) was smaller than that before the surgery (15.03 ± 7.39°) ( P &lt; 0.05 ), while the postoperative cervical sagittal vertical axis (cSVA) (21.33 ± 10.38 mm) and Japanese Orthopaedic Association (JOA) scores (16.95 ± 6.07 points) were greater than those before the surgery (14.66 ± 9.68 mm and 11.39 ± 4.28 points) ( P &lt; 0.05 ). Besides, the improvement rate of patients from the cSVA &gt;25 mm group (68.31%) was greater than the rate of the cSVA &lt;15 mm group (45.88%) and the cSVA within 15–25 mm group (47.29%) ( P &lt; 0.05 ). In conclusion, PLP-3DR could effectively improve the diagnostic effect of spine X-ray film images, with high sensitivity and specificity. Anterior cervical surgery had a good curative effect for patients with cervical spondylosis and was closely related to cervical parameters (cSVA, T1 slope, and C2-7Cobb). In addition, patients with cSVA&gt;25 mm could have the best postoperative rehabilitation effect.

Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model.

Patients with Parkinson's disease (PD) have impaired insulin signaling in the brain. Incretin hormones, including glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), can re-sensitize insulin signaling. In a recent phase II clinical trial, the first GLP-1 mimic, exendin-4, has shown reliable curative effect in patients with PD. DA-CH5 is a novel GLP-1/GIP receptor unimolecular co-agonist with a novel peptide sequence added to cross the blood-brain barrier. Here we showed that both exendin-4 and DA-CH5 protected against 6-hydroxydopamine (6-OHDA) cytotoxicity, inhibited apoptosis, improved mitogenesis and induced autophagy flux in SH-SY5Y cells via activation of the insulin receptor substrate-1 (IRS-1)/alpha serine/threonine-protein kinase (Akt)/cAMP response element-binding protein (CREB) pathway. We also found that DA-CH5 (10 nmol/kg) daily intraperitoneal administration for 30 days post-lesion alleviated motor dysfunction in rats and prevented stereotactic unilateral administration of 6-OHDA induced dopaminergic neurons loss in the substantia nigra pars compacta. However, DA-CH5 showed curative effects in reducing the levels of α-synuclein and the levels of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β). It was also more effective than exendin-4 in inhibiting apoptotic process and protecting mitochondrial functions. In addition, insulin resistance was largely alleviated and the expression of autophagy-related proteins was up-regulated in PD model rats after DA-CH5 treatment. These results in this study indicate DA-CH5 plays a therapeutic role in the 6-OHDA-unilaterally lesioned PD rat model and is superior to GLP-1 analogue exendin-4. The study was approved by the Animal Ethics Committee of Shanxi Medical University of China.

The role of bioelectrical impedance in the evaluation of bone mass in patients with advanced breast cancer

Rationale: Bioelectrical impedance analysis(BIA) of human body composition is a rapid, accurate and non-invasive examination method, which is widely used to evaluate the nutritional status and curative effect of patients with various diseases; the incidence of tumor bone metastasis in patients with advanced breast cancer is about 65%>75%, and patients with bone metastasis account for 27%>50% of the total patients in initial diagnosis. The main purpose of this study is to explore the role of BIA in evaluating bone mass in patients with advanced breast cancer.

De novo mutation of cancer-related genes associates with particular neurodevelopmental disorders.

Epidemiological studies have shown an increased prevalence of cancer in some patients with neurodevelopmental disorder (NDD); however, the genetic mechanisms regarding how cancer-related genes (CRGs) contribute to NDD remain unclear. We performed bioinformatic analyses on 219 CRGs from OMIM and de novo mutations (DNMs) from 16,498 patients with different NDDs and 3391 controls. Our results showed that autism spectrum disorder, undiagnosed neurodevelopmental disorder, congenital heart disease and intellectual disability, but not epileptic encephalopathy and schizophrenia, harboured significantly more putative functional DNMs in CRGs, compared with controls, providing genetic evidence supporting previous epidemiological surveys. We further detected 26 CRGs with recurrent putative functional DNMs that showed high expression in the human brain during the prenatal stage and in non-brain organs in adults. The proteins coded by the 26 CRGs and known NDD candidate genes formed a functional network that is involved in brain development and tumorigenesis. Overall, we proposed 39 cancer-targeting drugs that could be investigated for treating patients with NDD, which would be potentially cost-effective. In conclusion, DNMs contribute to specific NDDs and there may be a shared genetic basis between NDDs and cancer, highlighting the importance of considering cancer-targeting drugs with potential curative effects in patients with NDDs. KEY MESSAGES: • The contribution of DNMs in NDD is consistent with epidemiological surveys. • We highlighted 26 CRGs, including nine genes with more than five functional DNMs. • Specific expression patterns underlie the genetic mechanism of CRGs in NDD. • Specific functional networks underlie the genetic mechanism of CRGs in NDD. • The shared genetic aetiology suggests potential mutual treatment strategies.

3D Printing Hip Prostheses Offer Accurate Reconstruction, Stable Fixation, and Functional Recovery for Revision Total Hip Arthroplasty with Complex Acetabular Bone Defect

Complicated and large acetabular bone defects present the main challenges and difficulty in the revision of total hip arthroplasty (THA). This study aimed to explore the advantages of three-dimensional (3D) printing technology in the reconstruction of such acetabular bone defects. We retrospectively analyzed the prognosis of four severe bone defects around the acetabulum in three patients who were treated using 3D printing technology. Reconstruction of bone defect by conventional methods was difficult in these patients. In this endeavor, we used radiographic methods, related computer software such as Materialise's interactive medical image control system and Siemens NX software, and actual surgical experience to estimate defect volume, prosthesis stability, and installation accuracy, respectively. Moreover, a Harris hip score was obtained to evaluate limb function. It was found that bone defects could be adequately reconstructed using a 3D printing prosthesis, and its stability was reliable. The Harris hip score indicated a very good functional recovery in all three patients. In conclusion, 3D printing technology had a good therapeutic effect on both complex and large bone defects in the revision of THA. It was able to achieve good curative effects in patients with large bone defects.

Factors Associated with Dose Modification of Lenalidomide Plus Dexamethasone Therapy in Multiple Myeloma.

Long-term combination treatment with lenalidomide and low-dose dexamethasone is important to achieve a curative effect in patients with multiple myeloma (MM). In this study, the plasma concentration of lenalidomide was measured at 3 h after oral administration, when the drug is in the elimination phase and can be easily measured in outpatients, to identify factors that may lead to the discontinuation of this combination therapy. Patients were assigned to continuation or discontinuation of therapy groups, and the baseline characteristics of patients, lenalidomide concentration, and concentration/dose (C/D) ratios reflecting oral clearance were compared between the two groups. The efficacy and severity of adverse events were also compared. The results showed that patients who discontinued or modified treatment had low plasma concentrations of lenalidomide and C/D ratios, indicating high oral clearance of lenalidomide. The estimated creatinine clearance rate was negatively correlated with the C/D ratio. The plasma concentrations of lenalidomide were independent from kidney function and differed significantly among patients. Taken together, the results indicate that low plasma concentrations of lenalidomide and low C/D ratios may lead to discontinuation of combination therapy in patients with MM. This suggests that early measurement of lenalidomide plasma continuation would help to prevent discontinuation of therapy or a delay in modifying the dose of lenalidomide.

Correlation between effectual time and the curative effect in patients with all frequency descending sudden deafness after treatment.

To discuss the correlation between effectual time and the curative effect in patients with all frequency descending sudden deafness. According to effectual time, the subjects were divided into first week effectual group and second week effectual group and the curative effect of each group was compared. In patients with flat descent sudden deafness, the curative rate of the first week effectual group was higher than that of the second week effectual group, but there was no significant difference between the two groups (χ2 = 1.584, P = 0.208). Meanwhile, the total significant effective rate of the first week effectual group was higher than that of the second week effectual group, without obvious difference between the two groups (χ2 = 0.227, P = 0.634). Furthermore, in patients with total deafness type of sudden deafness, the curative rate of the first week effectual group was higher than that of the second week effectual group, showing no remarkable difference between the two groups (χ2 = 2.726, P = 0.099). Besides, there was no remarkable difference in the comparison of the total significant effective rate (χ2 = 2.933, P = 0.087), which was higher in the first week effectual group than that in the second week effectual group. The course of treatment should be at least 2 weeks in patients with all frequency descending sudden deafness after onset.

Observation on nystagmus characteristics and curative effect of patients with light cupulopathy

Objective:To describe the characteristics of nystagmus in patients with Light Cupulopathy and to investigate its therapeutic effect. Method:The Supine roll test in our hospital's otology clinic showed persistent DCPN in 26 patients, who received Supine roll test, null-point position test, and prone-supine test. All patients were treated with barbecue maneuver. The immediate, short-term and long-term effects of treatment were investigated. Result:Null-point position on one side was present in 26 patients. The angles ranged from 15-45°, with an average of （26.34±8.78）°. Spontaneous nystagmus was observed in 30.8% （ 8/26 ） patients. The nystagmus was directed to the affected side in prone position and to the healthy side in supine position. Seventy-three percent of the patients had strong nystagmus and 26.9% had weak nystagmus in the Supine roll test. All patients received Barbecue maneuver, and the recovery rates were 0, 42.3% and 88.5% immediately, 1 week and 1 month after treatment respectively. There was a statistical difference in the recovery rate among immediate, one week and one month later （P<0.01）. Conclusion:The nystagmus performance of patients with persistent DCPN conforms to the light cupula hypothesis. The lesion side can be determined according to the null-point position and prone-supine test. The lesion side cannot be determined by the intensity of nystagmus in the Supine roll test in some cases. Repositioning maneuver is ineffective in treating light cupulopathy, but the disease is self-limited.

Clinical observation of the efficacy and mechanism of the Wenfei Jiangzhuo formula in lung and kidney deficiency-type vascular dementia

To explore the clinical efficacy and mechanism of the Wenfei Jiangzhuo formula in lung and kidney deficiency-type vascular dementia. The study was conducted from March 2014 to March 2016. Eighty-four patients with lung and kidney deficiency-type vascular dementia were divided into the observation (n = 44) and control (n = 40) groups. The control group received oral donepezil hydrochloride, while the observation group received the Wenfei Jiangzhuo formula. The mini-mental state examination (MMSE) score, activities of daily living (ADL) scale score, and serum superoxide dismutase (SOD) and malondialdehyde (MDA) levels were assessed before and after treatment. The MMSE and ADL scale scores in the two groups significantly improved after treatment (P < 0.05); the scores in the observation group were significantly higher than those in the control group (P < 0.05). The SOD levels in both groups significantly improved, and there was a significant difference in the MDA levels in both groups (P < 0.05). The SOD and MDA levels improved more significantly in the observation group than in the control group (P < 0.05). The Wenfei Jiangzhuo formula has a good curative effect in patients with lung and kidney deficiency-type vascular dementia and may improve MMSE and ADL scale scores and patient satisfaction.

Application of ultrasound in the diagnosis of gastrointestinal tumors

Gastrointestinal tumors are common tumors in the digestive system. Early diagnosis of gastrointestinal tumors is the key to improve prognosis and curative effect of patients with tumors. Compared with other methods of examination and diagnosis, ultrasound examination has the advantages of simple operation, non-invasive, economical, and repeatable operation. With the advancement of ultrasound technology and the development of ultrasound contrast agents, ultrasound examination is more and more applied to gastrointestinal examination. Ultrasound cannot only observe the gastrointestinal wall, but also evaluate the surrounding lesions and metastases, as well as preoperative analysis and postoperative follow-up of gastrointestinal tumors. We reviewed the diagnostic applications of ultrasound in gastrointestinal tumors.

Effect of Shuxuening combined with edaravone on neurological function, serum VEGF and serum sVCAM-1 in patients with cerebral infarction

Objective To investigate the effects of Shuxuening injection combined with edaravone on neurological function, serum vascular endothelial growth factor (VEGF) and soluble vascular cell adhesion molecule-1 (sVCAM-1) in patients with cerebral infarction. Methods A total of 112 patients with cerebral infarction admitted to Zhejiang Provincial Armed Police Hospital from August 2016 to August 2018 were enrolled in the study.They were divided into observation group and control group according to the digital table, with 56 patients in each group.The two groups were given routine symptomatic treatment.On the basis of this, the control group was given edaravone treatment, and the observation group was given Shuxuening injection combined with edaravone.The changes of NIHSS, GCS score, serum NSE, NGF, NTF, VEGF and sVCAM-1 levels before and after treatment were compared between the two groups, and the clinical efficacy was compared. Results After treatment, the effective rate of the observation group was 92.86%(52/56), which was higher than that of the control group 73.21%(41/56), the difference was statistically significant (χ2=7.669, P<0.05). After treatment, the NIHSS score, NSE, sVCAM-1 of the two groups were decreased, which of the observation group were lower than those of the control group[(3.27±1.16)points vs.(6.32±2.12)points, (13.15±1.14)μg/L vs.(17.64±1.89)μg/L, (648.56±134.67)ng/mL vs.(1 078.36±131.23)ng/mL], while the GCS score, NGF, NTF, VEGF levels of the two groups were elevated, which of the observation group were higher than those of the control group[(13.64±3.86)points vs.(11.89±3.41)points, (76.13±8.72)μg/L vs.(68.24±7.25)μg/L, (5.14±0.36)μg/L vs.(3.74±0.29)μg/L, (831.51±91.67)ng/L vs.(752.83±91.24)ng/L], the differences were statistically significant (t=9.414, 2.543, 15.223, 5.205, 22.663, 4.552, 15.672, all P<0.05). Conclusion Shuxuening injection combined with edaravone can improve the curative effect of patients with cerebral infarction, improve neurological function, effectively improve serum VEGF level and decrease sVCAM-1 level.It is worthy of clinical application. Key words: Infarction, middle cerebral artery; Central nervous system; Vascular endothelial growth factors; Shuxuening; Edaravone

Clinical efficacy of anti-anxiety treatment in patients with arrhythmia

Objective To analyze the relationship between arrhythmias and anxiety, and to evaluate the effect of anti-anxiety treatment in patients with arrhythmias while given conventional treatment. Methods From February 2015 to February 2017, 111 patients with arrhythmia in the Fourth Hospital of Handan were selected in the study, and they were divided into treatment group(n=62) and control group(n=49) according to the admission to single and double wards.The control group was treated with conventional anti-arrhythmias drugs, while the treatment group was treated with low-dose of anti-anxiety drugs and conventional anti-arrhythmias drugs.The therapeutic effects, symptoms improvement time and adverse reactions were compared between the two groups. Results The effective rate in the treatment group was 96.8%(60/62), which in the control group was 77.6%(38/49), the difference was statistically significant between the two groups (χ2=9.78, P 0.05). Conclusion Arrhythmia has a certain relationship with anxiety.Anti-anxiety combined with anti-arrhythmic treatment can improve the curative effect of patients with arrhythmia and shorten the time when symptoms begin to improve. Key words: Arrhythmias, cardiac; Anti-arrhythmia agents; Anxiety; Anti-anxiety agents; Drug therapy, combination; Dose-response relationship, drug; Electrocardiography, ambulatory; Treatment outcome; Comparative study

Efficacy and safety of Yangzheng Xiaoji capsule combined with radiotherapy and chemotherapy in the treatment of gastric cancer and its influence on immune function

Objective To explore the efficacy and safety of Yangzheng Xiaoji capsule combined with radiotherapy and chemotherapy in the treatment of gastric cancer and its influence on immune function. Methods From October 2016 to October 2018, 80 patients with gastric cancer in the First People's Hospital of Yongkang were divided into two groups according to the random number table, with 40 cases in each group.The control group received conventional radiotherapy plus chemotherapy, namely oxaliplatin, 5-fluorouracil combined with calcium leucovorin conventional chemotherapy.The observation group received Yangzheng Xiaoji capsule on the basis of the control group.The clinical symptoms, objective tumor efficacy, TCM symptoms and signs score, safety, immune function of the two group were observed. Results The total effective rate of the observation group was 87.5%, which was significantly higher than 72.5% of the control group(χ2=3.52, P 0.05), and there were statistically significant differences in WBC, Hb and PLT of the control group before and after treatment(t=4.85, 3.64, 3.85, all P 0.05). There were no statistically significant differences in the levels of CD3＋, CD4＋, CD8＋, CD4＋/CD8＋ of the observation group before and after treatment(t=2.14, 2.08, 1.69, 2.47, all P>0.05), and compared with before treatment, the levels of CD3＋, CD4＋, CD4＋/CD8＋ of the control group were significantly decreased(t=5.12, 4.26, 5.38, all P<0.05), while the level of CD8＋ was significantly increased(t=3.86, P<0.05). After treatment, the levels of CD4＋, CD4＋, CD8＋, CD4＋/CD8＋ of the observation group were significantly better than those of the control group (t=4.79, 3.52, 4.28, 3.61, all P<0.05). Conclusion Yangzheng Xiaoji capsule combined with radiotherapy and chemotherapy can improve the curative effect of patients with gastric cancer, and it has high safety, less toxic and side effects, and can improve the immune function of patients with gastric cancer.It is worthy of promoting. Key words: Stomach neoplasms; Radiotherapy; Antineoplastic combined chemotherapy protocols; Medicine, Chinese Traditional; Signs and symptoms; Safety; Immunity; Comparative effectiveness research

622-P: Mechanism of Lin28a Promoting Vascular Smooth Muscle Cell Proliferation and Migration in Restenosis

Background: Restenosis (RS) after percutaneous transluminal angioplasty severely affects the curative effect of patients with lower extremity peripheral arterial disease. Our previous research indicated that abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) was the key factor in the RS plaque formation. The process of Lin28a regulating cell proliferation demonstrates similarities with the proliferation of VSMCs in RS. Here, we aim to explore the specific role of Lin28a in RS. Methods: To distinguish from atherosclerotic lesions, RS and atherosclerosis (AS) models were simultaneously established in type 2 diabetic rats. Lin28a level in VSMCs was detected by immunofluorescence double staining. The changes of VSMCs were determined when modulating Lin28a in vivo and vitro and downstream mechanism was further explored. Results: Thirty-two folds higher level of Lin28a was found in VSMCs in RS compared with AS (P&amp;lt;0.05). Overexpression of Lin28a significantly promoted proliferation and migration of VSMCs in vitro. Let7 was identified as the direct target of Lin28a; screening for Let7 subtypes by in vivo and vitro showed that Let7c, let7d, let7g, miR98 were negatively regulated by Lin28a in VSMCs. Among them, downregulation of Let7c markedly suppressed the VSMCs’ proliferation and migration accompanied by upregulation of Lin28a expression; while these effects were alleviated by overexpression of Let7c. Conclusion: The present study demonstrated that Lin28a is critically involved in the progression of RS via facilitating the proliferation and migration of VSMCs. A double negative feedback loop is often present in Let7 and Lin28a, thus Let7 subtypes were detected and Let7c could be the direct downstream of Lin28a in regulating VSMCs in RS. Combined with the fact that Lin28a was also regulated by Let7c, we inferred a feedback loop could be formed between Let7c and Lin28a. Above all, the interaction in Lin28a and Let7c may contribute to rapid formation of RS. Disclosure Q. Zhang: None. X. Zhou: None. X. Shen: None. S. Jiang: None. T. Xie: None. Z. Zou: None. C. Xu: None. R. Zhang: None. Y. Cui: None. P. Gong: None. J. Dong: None. L. Liao: None. Funding National Natural Science Foundation of China (81670757, 81570742, 81770822, 81800732); Shandong Provincial Natural Science Foundation of China (ZR2017LH025); Shandong Provincial Medicine and Health Science and Technology Development Program (2017WS461)

Curative effect of TACE combined with Antike in the treatment of primary liver cancer

Objective To evaluate the curative effect of transcatheter arterial chemoembolization (TACE) combined with Antike in the treatment of primary liver cancer. Methods The data of 72 patients with primary liver cancer (90 lesions in total) admitted to the First Affiliated Hospital of Hebei North University from August 2016 to December 2017 were retrospectively analyzed. All patients were divided into the observation group (n=36) and the control group (n=36) according to the therapeutic schedule. The patients in the control group (40 foci) were treated with TACE only, and the patients in the observation group (50 foci) received TCAE treatment, oral Antike capsules were taken at the same time with the frequency of three times a day, two capsules each time, and six weeks were planned for one treatment cycle. A total of four cycles were completed. All patients underwent CT enhancement scans one week before TACE treatment and six months after treatment. According to Response Evaluation Criteria in Solid Tumors, complete remission and partial remission were deemed as effectivity.The imaging data of the change of tumor size, postoperative tumor residual, tumor capsule growth and new intrahepatic metastases were analyzed and used to evaluate the curative effect of patients in the two groups. Results After 6 months of treatment, there were 39 effective lesions in the observation group and 23 in the control group. The total effective rate of the observation group was higher than that of the control group (78.0% vs. 57.5%), and the difference was statistically significant (χ2=4.357, P=0.037). Twenty-three neoplastic capsule lesions were detected in the observation group and 9 in the control group. The detection rate of neoplastic capsule was higher in the observation group than that in the control group (46.0% vs. 22.5%), and the difference was statistically significant (χ2=5.356, P=0.021). There were 27 residual tumors and 5 new intrahepatic metastases in the observation group, 30 residual tumors and 13 new intrahepatic metastases in the control group. The residual tumor rate and neohepatic metastasis rate in the observation group were lower than those in the control group (54.0% vs. 75.0%; 13.9% vs. 36.1%), and the differences were statistically significant (χ2=4.220, P=0.040; χ2=4.741, P=0.029). Conclusion TACE combined with Antike is safe and effective in the treatment of primary liver cancer. It can improve the total clinical efficiency, promote the growth of tumor capsule, and reduce the recurrence rate and metastasis rate of tumor. It is worthy of clinical application. Key words: Tomography, spiral computed; Liver neoplasms; Transcatheter arterial chemoembolization; Antike

Evaluation of curative effect of VNG and VEMP in patients with severe sudden hearing loss

Objective:To evaluate the relationship between vestibular function and curative effect in patients with severe sudden hearing loss.Method:The vestibular function of 79 patients with severe hearing loss was examined by video-nystagmography (VNG) and vestibular evoked myogenic potential (VEMP).Result:There were significant differences between the two test outcomes (P<0.01) in the severe group (27 cases). The effective rate of both VNG and VEMP normal group was 100%, the effective rate that both VNG and VEMP were abnormal was 41.7%. In the profound severe group (52 cases) there was also a significant difference between the two test results and the therapeutic effect (P<0.01). The effective rate of both VNG and VEMP normal group was 76.9%. The effective rate was 34.6% with both VNG and VEMP abnormal. There was no significant difference between hearing loss degree and curative effect.Conclusion:The VNG and VEMP examinations in patients with severe sudden hearing loss are valuable in evaluating the efficacy. Both VNG and VEMP were abnormal with the worst curative effect.