Coffee Cup Quality Research Articles

Predicting best planting location and coffee cup quality from chemical parameters: An evaluation of raw Arabica coffee beans from São Paulo over two harvests

Arabica coffee is one of the most consumed beverages in the world. The chemical components present in raw Arabica coffee beans (RACB) are directly related to the cup quality of the beverage. Environmental and genetic factors influence the content and profile of these components. Then, this work aimed to evaluate different chemical parameters of RACB from 3 varieties planted in 3 different experimental farms located within the “Alta Mogiana” terroir harvested in 2021 and 2022 to identify a better variety for each farm to produce a high cup quality Arabica coffee. The harvest period had a strong influence on most of the studied parameters because atypical weather conditions occurred in the 2021 harvest. The RACB harvested in 2022 yielded better results and supposedly will produce a beverage of high cup quality. Samples harvested in this period presented mainly average moisture levels closer to optimum (11.02 against 8.56 % in 2021); low total titratable acidity (98.00 against 169.75 mL 0.1 M NaOH/100 g in 2021); high amounts of free amino acids (0.96 against 0.93 g GAE/100 g in 2021), low amounts of CGA (4.27 against 4.85 g/100 g in 2021) and caffeine (1.08 against 1.76 g/100 g in 2021) and high amounts of trigonelline (1.12 against 0.96 g/100 g in 2021). The Rome Sudan variety had the best combination of chemical results, mainly when cultivated in Farm 2 in 2022, presenting high amounts of protein content (15.24 %) and free amino acids (0.96 g GAE/100 g), low total titratable acidity (98.3 mL 0.1 M NaOH/100 g), low amounts of CGA (4.55 g/100 g) and caffeine (1.29 g/100 g) and high amounts of trigonelline (1.11 g/100 g). The analysis of chemical compounds could predict the best farm to cultivate each variety studied and was a guide to foresee a higher cup quality of RACB beverages.

RENDIMIENTO Y CALIDAD FÍSICA DEL GRANO DE CAFÉ (Coffea arabica L.) VARIEDAD COSTA RICA 95

<p><strong>Background: </strong>Coffee is the second most consumed drink in the world, only after water. Quality characteristics are the most recurrent demands of consumers, which are of multifactorial origin and among which is agronomic management where soil nutrition or foliar nutrition of the plant is considered. <strong>Objective:</strong> To evaluate the yield and physical quality of the bean and cup of coffee (Coffea arabica L.) variety Costa Rica 95 by applying foliar fertilizer. <strong>Methodology: </strong>The evaluation was carried out in the La Laja community, municipality of Tlaltetela, Veracruz between 2018 and 2019. The age of the plantation used was four years and established with chalahuite shade (<em>Inga spuria</em> Humb. & Bonpl. ex Willd). The design used was a complete randomized block with two treatments (commercial control and treatment with foliar fertilization), with three repetitions each; the experimental unit consisted of a 300 m<sup>2</sup> plot and five coffee harvests were carried out, which were according to the number of flowerings during the 2018-2019 cycle. The physical evaluation was carried out according to the Official Mexican Standard NMX-F-162-SCFI-2008 and the NOM SCAA (2013). <strong>Results: </strong>The highest cherry yield and brix degrees were observed in the fertilized treatment with 12,897.8 kg ha<sup>-1</sup> and 20.06, respectively; in floats (waste) and snail (malformation), the highest weights were observed in the control treatment with 1,696.11 kg ha<sup>-1</sup> and 21.4 grams, respectively. The total sum of the sensory analysis was 81.3 and 85.2 for the treatments: control and fertilized, respectively. Higher numbers of grains were observed in sieves 19, 18 and 17 that are classified for the international scale. <strong>Implications: </strong>There is a positive effect of foliar fertilizer on the yield of cherry coffee and cup quality of Costa Rica coffee 95 and opens a range of possibilities to carry out a greater number of fertilization tests in the field, being able to compare an additional factor such as, for example, the climate (rain, drought, cold, heat, etc.). These studies should be carried out in the same area in which this experiment was carried out to compare whether the response shown is due solely to foliar fertilizer or there is an influence of climate, as well as management. It must be considered that the quality of coffee is multifactorial; in addition to environmental and genetic factors, there is management (nutrition, phytosanitary protection, pruning), harvest, among others. <strong>Conclusion: </strong>Foliar fertilization considerably improved the variables of interest evaluated in Costa Rica 95 coffee through the application of foliar fertilization, in addition to the fact that no primary or secondary defects were significantly present.</p>

Effect of processing methods on physicochemical and cup quality of coffee at Jimma, Ethiopia

Coffee quality is a complex attribute influenced by a variety of factors, including postharvest processing methods. The goal of this study was to investigate the impact of coffee processing methods on coffee quality (raw, cup, and biochemical makeup) in Jimma, which represents the midland areas. Coffee samples were collected for Jimma agricultural research center and processed with three methods (washed, Semi-washed and dry process). The result indicated that washed coffee beans scored significantly the highest color (13.43) and odor [10] score than the other processing methods. The statistically best raw quality score (35.57 %) was therefore reported for washed coffee in Jimma. Cup quality attributes however were not significantly affected by processing methods. Among chemical composition studied, lipid was significantly affected by processing methods and highest lipid 13.74 and 13.17 g/100g was reported for semi-washed and washed coffee beans respectively. Generally, washed and semi-washed coffee were preferable in terms of bean color and odor quality. Correlations were found among cup quality attributes and chemical compositions. However, further research into the accessibility and economics of coffee processing methods in the area is necessary.

Physicochemical and cup quality of coffee as affected by processing methods and growing locations

ABSTRACT Coffee processing methods have a noteworthy impact on coffee quality. The aim of this study was to investigate the influence of coffee processing methods on raw and cup quality at Jimma, Gera and Tepi locations. Accordingly, the highest total raw quality (36.34 and 36.39%) was recorded under Tepi locations for washed and semi-washed coffee respectively followed by 35.57% for washed coffee in Jimma and 35.07% for washed coffee in Gera. However, the cup quality of dry processed coffee is significantly higher at Tepi (47.24) and Jimma (45.07) locations. But, under Gera, washed and semi-washed coffee scored the highest cup quality (45.37 and 45.07%), respectively. Highest caffeine (1.51 and 1.55 g/100 g) and lipid (14.46–15.03 g/100 g) were recorded for Tepi and Gera, respectively, which were attributed to location. In general, all processing methods can result in acceptable coffee quality, provided that carried out in accordance with expert advice.

Metataxonomic Identification of Microorganisms during the Coffee Fermentation Process in Colombian Farms (Cesar Department).

The metataxonomic diversity and microbial composition of microorganisms during the coffee fermentation process as well as their relationship with coffee quality were determined across 20 farms in the department of Cesar, Colombia, by sampling coffee fruits from Coffea arabica; Var. Castillo General®, Var. Colombia, and Var. Cenicafé 1. In each farm, the fruits were processed and the fermentation process took place between 10 and 42 h following this. Three samples of mucilage and washed coffee seeds were collected per farm during the fermentation process. The microorganisms present in the mucilage were identified using metataxonomic methods by amplifying the 16S rRNA gene for bacteria and ITS for fungi. The microorganisms' morphotypes were isolated and identified. The analysis of bacteria allowed for the identification of the following genera: Gluconobacter, Leuconostoc, Acetobacter, Frateuria, Pantoea, Pseudomonas, Tatumella, and Weisella, as well as unclassified enterobacteria; the Lactobacillacea and Secundilactobacillus families were only identified in the Var. Cenicafé 1. For fungi, the top 11 genera and families found included Hanseniaspora, Candida, Meyerozyma, Wickerhamomyces, Pichia, f-Saccharomycodaceae, f-Nectriciae, unclassified fungi, and Saccharomycetaceae, which were only found in Cenicafé 1. A total of 92% of the coffee samples obtained scored between 80.1 and 84.9, indicating "Very Good" coffee (Specialty Coffee Association (SCA) scale). Farms with the longest fermentation times showed better coffee attributes related to acidity, fragrance, and aroma. During coffee fermentation, there is a central microbiome. The differences between the microorganisms' genera could be influenced by the coffee variety, while the specific conditions of each farm (i.e., altitude and temperature) and its fermentation processes could determine the proportions of and interactions between the microbial groups that favor the sensory characteristics responsible for coffee cup quality.

Impact of single and mixed cultures of yeasts and lactic acid bacteria in coffee fermentation and cup quality

AbstractSaccharomyces cerevisiae LBTF 21.3, Pichia manshurica LBTF 21.1, and Leuconostoc lactis LBTF 21.1 were used as single and mixed cultures in coffee fermentations. Fermentations carried out in glass jars with P. manshurica LBTF 21.1/Leuc. lactis LBTF 21.1 showed higher fermentation rates than S. cerevisiae LBTF 21.3/P. manshurica LBTF 21.1 or S. cerevisiae LBTF 21.3/Leuc. lactis LBTF 21.1. The highest cup quality was reached with P. manshurica LBTF 21.1/Leuc. lactis LBTF 21.1 (83.0 points). Full factorial design (22) carried out with P. manshurica LBTF 21.1 revealed that the higher proportion of water added before the fermentation resulted in higher production of acetic acid. A contrary effect of this variable was observed on the production of lactic acid with Leuc. lactis LBTF 21.1. Fermentations conducted in bioreactor evidenced the production of acetaldehyde by P. manshurica LBTF 21.1/Leuc. lactis LBTF 21.1, P. manshurica LBTF 21.1, and Leuc. lactis LBTF 21.1. Additionally, the highest cup quality was reached in fermentations with P. manshurica LBTF 21.1 (82.5 points) followed by Leuc. lactis LBTF 21.1 (81.5 points) and P. manshurica LBTF 21.1/Leuc. lactis LBTF 21.1 (81.0 points). Fermentations of coffee with single and mixed cultures are interesting strategies for the improvement of the sensory quality.

Optimizing drip fertigation at different periods to improve yield, volatile compounds and cup quality of Arabica coffee.

How to improve and regulate coffee bean yield and quality through split fertilization in the whole life cycle of coffee is still unclear and deserves further study. A field experiment of 5-year-old Arabica coffee trees was conducted for 2 consecutive years from 2020 to 2022. The fertilizer (750kg ha-1 year-1, N-P2O5-K2O:20%-20%-20%) was split in three times at early flowering (FL), the berry expansion (BE), and the berry ripening (BR). Taking equal fertilization throughout the growth cycle (FL250BE250BR250) as the control check, variable fertilizations including FL150BE250BR350, FL150BE350BR250, FL250BE150BR350, FL250BE350BR150, FL350BE150BR250, and FL350BE250BR150. Leaf net photosynthetic rate (A net), stomatal conductance (g s), transpiration rate (T r), leaf water use efficiency (LWUE), carboxylation efficiency (CE), partial factor productivity of fertilizer (PFP), bean yield, crop water use efficiency (WUE), bean nutrients, volatile compounds and cup quality, and the correlation of nutrients with volatile compounds and cup quality was evaluated. FL350BE250BR150 had the maximum A net and g s, followed by FL250BE350BR150. The highest dry bean yield and WUE were obtained from FL250BE350BR150, which increased by 8.86% and 8.47% compared with FL250BE250BR250 in two-year average. The ash, total sugar, fat, protein, caffeine and chlorogenic acid in FL250BE350BR150 were 6.47%, 9.48%, 3.60%, 14.02%, 4.85% and 15.42% higher than FL250BE250BR250. Cluster analysis indicated FL150BE350BR250, FL250BE350BR150, FL350BE150BR250 and FL350BE250BR150 under medium roasted degree increased pyrazines, esters, ketones and furans, FL150BE350BR250 and FL250BE350BR150 under dark roasted degree increased ketones and furans. The aroma, flavor, acidity and overall score of medium roasted coffee were higher than dark roasted coffee, while the body score of dark roasted coffee was higher than medium roasted coffee. The nutrient contents were correlated with the volatile compounds and cup quality. TOPSIS indicated that FL250BE350BR150 was the optimal fertilization mode in the xerothermic regions. The obtained optimum fertilization mode can provide a scientific basis for coffee fertilization optimization and management.

Effects of shade tree species on soil biogeochemistry and coffee bean quality in plantation coffee

Shade trees are used in many coffee production systems across the globe. Beyond the benefits on biodiversity conservation, climate buffering, carbon sequestration and pathogen regulation, shade trees can impact the soil nutrient status via, for instance, litter inputs and nitrogen fixation. Since soil nutrients affect coffee quality and taste, there is also a potential indirect effect of shade tree species on coffee quality. Yet, in spite of the potentially large impact of shade tree species, quantitative data on the effects of shade trees on (i) soil biogeochemistry and (ii) the associated coffee bean quality remain scarce. To what extent four widely used shade trees species (Acacia abyssinica L., Albizia gummifera L., Cordia africana L. and Croton macrostachyus L.) in a plantation coffee agroforestry system impact soil biogeochemistry, and how this in turn affects coffee quality, measured as cupping scores and bean size. A significant negative impact of N-fixing shade tree species on soil pH and base cation concentrations was found. Plant-available and total phosphorus was enhanced by the presence of Albizia gummifera L. Thus, the present findings demonstrate that careful selection and integration of shade tree species such as Acacia abyssinica L. and Albizia gummifera L. into coffee production systems is a good practice for sustaining soil chemical properties in coffee agroecosystem. Despite the impacts on soil chemical characteristics, the shade tree species had no effect on coffee cup quality but did affect the bean mass. In this particular study, an attempt was made to quantify the impacts of widely used shade tree species on soil biogeochemistry and the subsequent effect on coffee bean quality in a plantation agroforestry system over the course of one season in southwest Ethiopia. However, it might be feasible to accommodate both relatively sparse time-series experimental data consisting of coffee farms from plantations and smallholders, which needs to be the goal of future research to accurately examine the impacts on the outcome variables.

Effect of Three Post-Harvest Methods at Different Altitudes on the Organoleptic Quality of C. canephora Coffee

C. canephora (syn. C. robusta) is distinctive due to its rising industrial value and pathogen resistance. Both altitude and post-harvest methods influence coffee cup quality; however, modest information is known about this coffee species. Therefore, the aim of this study was to determine the relationship between four different altitudes and post-harvest processes (dry, honey, and wet) to the improvement of the organoleptic quality of the C. canephora congolensis and conilon drink. For dry processing, congolensis and conilon showed the lowest scores in terms of fragrance/aroma, flavour, aftertaste, salt–acid, bitter–sweet, and body. Above 625 m, coffees from dry, honey, and wet processes increased scores in their sensory attributes, but there was no difference at such high altitudes when comparing post-harvest samples. Dry-processed coffee samples had total scores over 80 points at high altitudes. Conilon was perceived to have the best sensory attributes at high altitudes using honey processing. In general, the wet-processed congolensis and conilon samples had a tastier profile than dry-processed ones.

Profile of Bioactive Compounds, Aromas, and Cup Quality of Excelsa Coffee (Coffea liberica var. dewevrei) Prepared from Diverse Postharvest Processes.

The study is aimed at evaluating bioactive compounds, volatile compounds, and cup quality of Excelsa coffee (Coffea liberica var. dewevrei) from different postharvest processing procedures, i.e., natural, honey, semiwashed, and wine. The green beans from each procedure were roasted at light to medium levels. Sample analysis was performed using HPLC and GC-MS instruments for bioactive compounds and volatile compounds, respectively, followed by a cupping test. As the results, postharvest processing significantly altered content of bioactive compounds (caffeoylquinic acids (CQAs) and alkaloids) in Excelsa green beans; the lowest quantity of CQAs and alkaloids was found in wine and semiwashed green beans, respectively. Significant degradation of 5-CQA and its transformation to 3-CQA and 4-CQA occurred in all light-medium roasting levels. In general, alkaloids were stable during roasting, and only trigonelline was slightly declined. Roasting process also generated 17 potent volatile compounds in Excelsa beans including 2-ethylpyrazine, 2,3-dimethylpyrazine, 2,5-dimethylpyrazine, 2,5-dimethyl-3-ethylpyrazine, guaiacol, 4-vinyl-guaiacol, and isovaleric acid and its esters. Furthermore, cupping test revealed that Excelsa coffee samples obtained from different postharvest processing were categorized as very good coffee (cupping score > 80). This finding may encourage the commercialization of all observed Excelsa coffee beans.

Influence of pre-and post-harvest factors on the organoleptic and physicochemical quality of coffee: a short review.

The coffee quality is affected by 40% pre-harvest, 40% post-harvest, and 20% export handling. Besides, future risks for the coffee industry are related with climate change and increased pathogens. Considering the importance of the aroma profile and unique flavor of Arabica coffee, most literature focuses on this variety because of the high market share; however, nowadays, Robusta coffee stands out for its increasing industrial value and resistance to drought. In this review, both species are emphasized, highlighting sensory aspects of possible new products mixed with a higher proportion of Robusta given market trends for bitter beverages. In the present work, a systematic search of peer-reviewed literature evaluates how the coffee cup quality and physicochemical characteristics of Robusta and Arabica are influenced by environmental, agronomic, and further processing factors.

UV spectroscopy for discrimination of two arabica coffee cultivars in West Java Indonesia: a feasibility study

Arabica coffee variety is one of the key parameters that highly influence the cup quality of arabica coffee. The objective of this current research is to evaluate the possible application of UV spectroscopy for the discrimination of arabica coffee with different cultivars. Green beans from two arabica coffee cultivars (Coffea arabica) of Typica and Sigarar Utang were collected from the same origin in Papandayan mountain West Java, Indonesia. The samples were subjected to the same postharvest treatments (wet cherry processing method). All samples were roasted in medium roasting with 200°C for 16 minutes using a portable roasting machine. A total of 40 samples of Typica and Sigarar Utang were provided by weighing 1 gram of coffee powder (mesh 40) for each sample. The extraction of coffee samples was performed based on previously reported work. The UV-visible spectral data of aqueous coffee samples were acquired by using a benchtop of UV-vis spectrometer in the range of 190-1100 nm. The first two PCs (principal components) with 94% of CEV (cumulative explained variance) could be used to separate between the Typica and Sigarar Utang samples. The Typica samples were situated on the left of PC1 (PC1<0) and Sigarar Utang samples were on the right of PC1 (PC1>0). In the future, it is promising to apply UV spectroscopy for simple and reliable discrimination of arabica coffee cultivar.

Shaded-Coffee: A Nature-Based Strategy for Coffee Production Under Climate Change? A Review

Coffee is deemed to be a high-risk crop in light of upcoming climate changes. Agroforestry practices have been proposed as a nature-based strategy for coffee farmers to mitigate and adapt to future climates. However, with agroforestry systems comes shade, a highly contentious factor for coffee production in terms of potential yield reduction, as well as additional management needs and interactions between shade trees and pest and disease. In this review, we summarize recent research relating to the effects of shade on (i) farmers' use and perceptions, (ii) the coffee microenvironment, (iii) pest and disease incidence, (iv) carbon assimilation and phenology of coffee plants, (v) coffee quality attributes (evaluated by coffee bean size, biochemical compounds, and cup quality tests), (vi) breeding of new Arabica coffee F1 hybrids and Robusta clones for future agroforestry systems, and (vii) coffee production under climate change. Through this work, we begin to decipher whether shaded systems are a feasible strategy to improve the coffee crop sustainability in anticipation of challenging climate conditions. Further research is proposed for developing new coffee varieties adapted to agroforestry systems (exhibiting traits suitable for climate stressors), refining extension tools by selecting locally-adapted shade trees species and developing policy and economic incentives enabling the adoption of sustainable agroforestry practices.

Sensory and Physicochemical Characteristics of Two Common Roast Defects in Robusta Coffee

Roasting is an important coffee processing step to generate coffee aroma and flavor. Because roasting is time-temperature dependent, the variation of time and temperature applied may influence the structural properties, visual appearance, and chemistry of coffee. Improper roasting creates roast defects that reduce coffee quality and acceptance. Despite this importance, studies on coffee roast defects, particularly in Robusta coffee is limited. This study aims to characterize two common roast defects, i.e., underdeveloped and overdeveloped, compared with medium roast in Robusta coffee. Sensory evaluation by trained panelists and physicochemical evaluation reveal that the two common roast defect in Robusta coffee can be distinguished clearly through differences in sensory (aroma defect) characteristics as well as physicochemical properties. The overdeveloped roast defect produced darker coffee with the highest pH and total dissolve solids (TDS), and can be characterized by pyridine, furan, phenol and pyrrole derivatives. The carbony and ashy notes of the overdeveloped coffee were potentially contributed by phenol and polyphenol derivatives. In contrast to the overdeveloped coffee, the underdeveloped coffee is markedly characterized by higher concentration of aliphatic acids and higher concentration of pyrazines that contributes to raw nut-like notes. The combination of time and temperature during roasting influences the breakdown of chemical compounds through complex mechanisms involving proteins, carbohydrates and polyphenols degradation. Thus, roasting process variations that determine coffee cup quality and in turn drive consumer acceptance should be controlled.

Genetic Variability and Correlation of Biochemical and Sensory Characteristics of Coffee

Organoleptic and biochemical attributes in the coffee bean determine the final cup quality of coffee which is a critical factor in the price determination of coffee in the market. The study aimed at determining the genetic variability of the green coffee bean. The trial sites were located at Siaya and Busia counties in Kenya. Nineteen different genotypes were established and included Arabusta coffee hybrids, backcrosses of Arabica to tetraploid Robusta, Arabica coffee, Robusta coffee, and Arabusta coffee. Randomized Complete Block Design with three replications in each site was used in conducting the experiment. The coffee beans were harvested in the year 2018 and extraction and calculation of sucrose, trigonelline, caffeine, and chlorogenic acids was carried using the recommended methods. The cupping procedure involved the use of five judges in assessing the flavor, aroma, balance, overall standard, acidity, body, and aftertaste of the roasted coffee beans. The sensory evaluation used the Specialty Coffee Association (SCA) method. There were significant variations recorded for the traits that were measured. All the traits were highly heritable registering values of &amp;gt; 50% for heritability whereby, caffeine and oil were highly heritable traits with 90.8% and 88.9% respectively. Oil had a high phenotypic coefficient of variation, genotypic variation, and response values when compared to the other traits. All the organoleptic traits were positively correlated with sucrose, trigonelline, and oil but the correlation with caffeine and chlorogenic acids was negative. The genotypic effects contributed largely to the high heritability recorded with a low influence from the environmental factors.

Effect of the Post-Harvest Processing on Protein Modification in Green Coffee Beans by Phenolic Compounds.

The protein fraction, important for coffee cup quality, is modified during post-harvest treatment prior to roasting. Proteins may interact with phenolic compounds, which constitute the major metabolites of coffee, where the processing affects these interactions. This allows the hypothesis that the proteins are denatured and modified via enzymatic and/or redox activation steps. The present study was initiated to encompass changes in the protein fraction. The investigations were limited to major storage protein of green coffee beans. Fourteen Coffea arabica samples from various processing methods and countries were used. Different extraction protocols were compared to maintain the status quo of the protein modification. The extracts contained about 4–8 µg of chlorogenic acid derivatives per mg of extracted protein. High-resolution chromatography with multiple reaction monitoring was used to detect lysine modifications in the coffee protein. Marker peptides were allocated for the storage protein of the coffee beans. Among these, the modified peptides K.FFLANGPQQGGK.E and R.LGGK.T of the α-chain and R.ITTVNSQK.I and K.VFDDEVK.Q of β-chain were detected. Results showed a significant increase (p < 0.05) of modified peptides from wet processed green beans as compared to the dry ones. The present study contributes to a better understanding of the influence of the different processing methods on protein quality and its role in the scope of coffee cup quality and aroma.

Cup quality profiles of Robusta coffee wilt disease resistant varieties grown in three agro-ecologies in Uganda.

The recently developed Robusta coffee wilt disease resistant (CWD-r) varieties in Uganda outperform the local landraces, both in yield and resilience. However, their uptake has been slow due to limited information on their cup worth. This study profiled the cup worth of the five most commonly grown CWD-r across the Lake Victoria Crescent, Western Mid-altitude farmland and Central Wooded Savannah agro-ecologies. Significant correlations (P ≤ 0.05) were observed between soil nutrients and coffee bean size but this was not the case for biochemical and cup quality. The proportion of coffee beans retained on screen 15; minimum acceptable size through coffee commercial markets, ranged from 58.09% in Mukono to 92.49% in Mityana. Interestingly, the bean size of variety KR4 was hardly influenced by environmental variations, with portions of beans retained on screen 15 being relatively the same (80.30% Ibanda, 89.50% Mukono, 98.20% Mityana). Coffee cup quality for most of the varieties was scored as premium (70-79%) across three agro-ecologies, with the exception of KR4, which was scored specialty grade (≥80%). Coffee blends generated were used to make coffee products with specialty score (82.25%) and a distinctive aroma complex. In this study, blends of CWD-r resulted in superior cup scores (76-82%). These findings show that CWD-r varieties have a high cup worth with potential for wide adaptation in Uganda's Robusta coffee growing agro-ecologies. Most importantly, variety KR4 has resilience across three agro-ecologies with a consistent high bean size and superior cup quality, making it a candidate variety for the market and breeding. © 2021 Society of Chemical Industry.

Influence of location, elevation gradients, processing methods, and soil quality on the physical and cup quality of coffee in the Kafa Biosphere Reserve of SW Ethiopia

In-depth forest coffee cup quality assessments have not been conducted yet on Kafa Biosphere Reserve coffees. Hence, the influence of location, elevation gradient, and processing methods on coffee bean physical and sensorial qualities, and the relationship between soil and coffee quality variables were studied in 2017. Districts (Gimbo, Gawata, and Decha), elevation gradient ranges (low-<1600, mid-1600-1800, and high->1800 m a.s.l), and processing methods (wet, semi-wet, and dry) were taken as factors. Preliminary coffee quality assessment data was collected from cup quality analysis of coffee beans obtained from the combination of the three factors where four replicate samples were taken for each of the combinations. The effect of location was only significant for bean moisture content and there was no significant (P > 0.05) difference among locations for above 14 screen retention and preliminary cup quality variables (odor, raw, acidity, body, flavor, cup, total, and grade). The elevation gradient had a significant (P < 0.001) effect on the scores of above 14 screen retention and most of the cup quality variables, but not on moisture content and odor. The effect of the coffee processing method was not significant (P > 0.05) on most of the preliminary cup quality variables, but it was significant for moisture content (P < 0.001), odor (P < 0.05), and overall raw (P < 0.01) scores. Screen retention was decreased with increasing elevation gradient but with better quality. High elevation coffees processed with the dry method gave better raw (38.5–40 ​%) and cup (48.00–51.75 ​%) quality scores. Except for high soil molybdenum and clay percentage, reduced amounts of most of the soil nutrients, pH, and silt percentage were important for better forest coffee quality. Since each sampled forest had diverse shade types and densities, a further investigation that includes the component of shade is strongly recommended in future studies.

The effect of drying temperature on cup quality of coffee subjected to mechanical drying

The objective of the work was to study the effect of drying temperature on cup quality of the robusta coffee subjected to mechanical drying in comparison with conventional sun drying. The robusta coffee processed by wet (parchment coffee) and dry (cherry coffee) methods were subjected to drying at different temperature regimes (40 oC, 50 oC and 60 oC) in a rotary mechanical dryer. The results of the study indicated that as the drying temperature increased, the time of drying reduced. Sun drying of parchment coffee took 48 hours (approximately seven days) to attain the desired moisture content of 11-12 per cent, while mechanical drying reduced the drying time to 16 to 24 hours. Similarly, cherry coffee subjected to sun drying took 88 hours (approximately 15 days), while mechanical drying reduced the drying time to 32 to 48 hours. The cup quality rating of coffee dried by different drying methods revealed that sun-dried robusta parchment coffee scored the highest cup rating. As the drying temperature increased, the cup quality ratings decreased. A similar cup quality rating was also observed with cherry coffee. These results indicate a considerable reduction of drying time when coffee beans are dried in a mechanical dryer. However, there is a need to regulate the drying temperature, which otherwise would negatively impact the quality of coffee. The drying temperature should not exceed 40oC for preserving the innate quality of robusta coffee because the high drying rates provoked by high temperatures can cause damage to the coffee quality due to the damage caused to the cell membranes. Overall, mechanical drying is more advantageous to sun drying in-terms of drying hours (indirectly reduces dependency on manpower) and preservation of innate quality of the coffee.

Lipidomic and metabolomic profiles of Coffea canephora L. beans cultivated in Southwestern Nigeria

Coffee (Coffea spp.) is one of the most popular refreshing beverages globally. Coffee lipid diversity has untapped potential for improving coffee marketability because lipids contribute significantly to both the health benefits and cup quality of coffee. However, in spite of its potential importance, there have not been extensive studies of lipids among C. canephora genotypes. In this study, ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS) profiling of lipid molecules was performed for 30 genotypes consisting of 15 cultivated and 15 conserved genotypes of C. canephora in Southwestern Nigeria. We identified nine classes of lipids in the 30 genotypes which belong to the 'Niaouli', 'Kouillou' and 'Java Robusta' group: among these, the most abundant lipid class was the triacylglycerols, followed by the fatty acyls group. Although 'Niaouli' diverged from the 'Kouillou' and 'Java Robusta' genotypes when their lipid profiles were compared, there was greater similarity in their lipid composition by multivariate analysis, compared to that observed when their primary metabolites and especially their secondary metabolite profiles were examined. However, distinctions could be made among genotypes. Members of the fatty acyls group had the greatest power to discriminate among genotypes, however, lipids that were low in abundance e.g. a cholesterol ester (20:3), and phosphotidylethanolamine (34:0) were also helpful to understand the relationships among C. canephora genotypes. The divergent lipid profiles identified among the C. canephora genotypes, correlated with their Single Nucleotide Polymorphism grouping as assessed by genotype-by-sequencing, and will be exploited to improve coffee cup quality.