Cultivation Mode Research Articles

Exploration and Practice of “Four Integration and Four Innovation” Specialized and Creative Integration Talent Cultivation Mode: Taking Chongqing Energy Vocational College as an Example

With the release of China Education Modernization 2035, education modernization has become a national strategic goal. In this context, Chongqing Energy Vocational College is actively exploring the “four integration and four innovation” specialized and creative integration talent cultivation mode, aiming at cultivating high-quality talents with innovative spirit and practical skills. This paper analyzes the status quo and challenges of talent cultivation in higher vocational education, puts forward the main practices of innovation-driven, creation and research, cross-border integration, and innovative practice, and has achieved remarkable results. Additionally, it summarizes the experience revelation and provides a reference for other higher vocational colleges and universities.

Research on Foreign Language Talent Cultivation Mode Based on the Concept of Language Security

Language security is an important part of national security, and in the current complex international environment, the issue of language security is becoming more and more prominent and has become an unignorable part of national security strategy. As the bridge and link of international communication, foreign language talents’ language skills and the level of national security awareness directly affect our country’s international image and the effect of international communication. Therefore, the establishment of a foreign language talent cultivation mode based on the concept of language security is not only an important way to improve the quality of foreign language education but also a realistic need to safeguard national security and promote international exchanges. Starting from the influence of the language security concept on foreign language talent cultivation, this paper analyzes the foreign language talent cultivation mode based on the language security concept, with a view to providing new ideas and methods for the development of foreign language education.

Application of Random Forest Algorithm in English Innovative Talent Cultivation Mode in Colleges and Universities

This research investigates the integration of the Random Forest Algorithm within the framework of English Innovative Talent Cultivation Mode in higher education institutions. The study uses a performance evaluation approach to examine the algorithm's impact on student learning outcomes and instructional methodologies. The algorithm's usefulness in improving outcomes and personalizing instructional approaches is examined using descriptive statistical analysis. The findings demonstrate the algorithm's potential to change English language education by providing useful insights into student performance that can drive instructional decisions. This study emphasizes the relevance of refining talent development tactics and cultivating innovative English language skills among students, thus contributing to the growth of educational practices in colleges and universities. As a result, using the Random Forest Algorithm in this scenario has the potential to revolutionize teaching practices while also encouraging a culture of continual improvement and innovation in English language acquisition.

Self-controlled in silico gene knockdown strategies to enhance the sustainable production of heterologous terpenoid by Saccharomyces cerevisiae

Microbial bioengineering is a growing field for producing plant natural products (PNPs) in recent decades, using heterologous metabolic pathways in host cells. Once heterologous metabolic pathways have been introduced into host cells, traditional metabolic engineering techniques are employed to enhance the productivity and yield of PNP biosynthetic routes, as well as to manage competing pathways. The advent of computational biology has marked the beginning of a novel epoch in strain design through in silico methods. These methods utilize genome-scale metabolic models (GEMs) and flux optimization algorithms to facilitate rational design across the entire cellular metabolic network. However, the implementation of in silico strategies can often result in an uneven distribution of metabolic fluxes due to the rigid knocking out of endogenous genes, which can impede cell growth and ultimately impact the accumulation of target products. In this study, we creatively utilized synthetic biology to refine in silico strain design for efficient PNPs production. OptKnock simulation was performed on the GEM of Saccharomyces cerevisiae OA07, an engineered strain for oleanolic acid (OA) bioproduction that has been reported previously. The simulation predicted that the single deletion of fol1, fol2, fol3, abz1, and abz2, or a combined knockout of hfd1, ald2 and ald3 could improve its OA production. Consequently, strains EK1∼EK7 were constructed and cultivated. EK3 (OA07△fol3), EK5 (OA07△abz1), and EK6 (OA07△abz2) had significantly higher OA titers in a batch cultivation compared to the original strain OA07. However, these increases were less pronounced in the fed-batch mode, indicating that gene deletion did not support sustainable OA production. To address this, we designed a negative feedback circuit regulated by malonyl-CoA, a growth-associated intermediate whose synthesis served as a bypass to OA synthesis, at fol3, abz1, abz2, and at acetyl-CoA carboxylase-encoding gene acc1, to dynamically and autonomously regulate the expression of these genes in OA07. The constructed strains R_3A, R_5A and R_6A had significantly higher OA titers than the initial strain and the responding gene-knockout mutants in either batch or fed-batch culture modes. Among them, strain R_3A stand out with the highest OA titer reported to date. Its OA titer doubled that of the initial strain in the flask-level fed-batch cultivation, and achieved at 1.23 ± 0.04 g L−1 in 96 h in the fermenter-level fed-batch mode. This indicated that the integration of optimization algorithm and synthetic biology approaches was efficiently rational for PNP-producing strain design.

Development of generic metabolic Raman calibration models using solution titration in aqueous phase and data augmentation for in-line cell culture analysis.

This study presents a novel approach for developing generic metabolic Raman calibration models for in-line cell culture analysis using glucose and lactate stock solution titration in an aqueous phase and data augmentation techniques. First, a successful set-up of the titration method was achieved by adding glucose or lactate solution at several different constant rates into the aqueous phase of a bench-top bioreactor. Subsequently, the in-line glucose and lactate concentration were calculated and interpolated based on the rate of glucose and lactate addition, enabling data augmentation and enhancing the robustness of the metabolic calibration model. Nine different combinations of spectra pretreatment, wavenumber range selection, and number of latent variables were evaluated and optimized using aqueous titration data as training set and a historical cell culture data set as validation and prediction set. Finally, Raman spectroscopy data collected from 11 historical cell culture batches (spanning four culture modes and scales ranging from 3to 200 L) were utilized to predict the corresponding glucose and lactate values. The results demonstrated a high prediction accuracy, with an average root mean square errors of predictionof 0.65 g/L for glucose, and 0.48 g/L for lactate. This innovative method establishes a generic metabolic calibration model, and its applicability can be extended to other metabolites, reducing the cost of deploying real-time cell culture monitoring using Raman spectroscopy in bioprocesses.

Effects of long-term positioning tillage on13C assimilate distribution and grain yield formation in wheat

To provide a theoretical basis and technical support for the high-yield and high-efficiency production of wheat, we examined the effects of different tillage patterns on wheat grain yield of Jimai 22 and the physiological mechanisms in an experiment with three treatments: 14 years in rotary tillage (R), minimal and no tillage (S), and minimal and no tillage with a 2-year subsoiling interval (SS). We assessed the light interception by wheat plant canopy, the distribution of photosynthate transport, and grain yield for the three cultivation modes. The results showed that leaf area index was significantly higher for SS treatment than the other treatments at 14-28 days after anthesis. The interception rate and amount of photosynthetically active radiation in the upper and middle layers of wheat canopy were significantly higher for SS treatment than R and S treatments at 21 days after anthesis. The contribution rate of grain assimilation and the distribution proportion of 13C assimilated in grain, and the maximum and average filling rates, were the highest under SS treatment. The 1000-kernel weight for SS treatment increased by 8.7% and 9.6%, and the grain yield increased by 14.2% and 19.4% compared with R and S treatments, respectively. SS treatment significantly improved light energy utilization by wheat canopy, promoted the accumulation and transport of dry matter, increased the grain-filling rate, increased grain weight, which together contributed to the highest grain yield. Therefore, SS was the optimal tillage pattern under the conditions of this experiment.

Innovation and Practice of “Job-Course-Competition-Certification” Professional Talents’ Cultivation Model in Tourism Major under the Background of Digitalization Era

The rapid development of new digital economies such as artificial intelligence, cloud computing, and big data is profoundly changing various industries, especially the tourism industry. The digital upgrade has promoted the transformation and development of the tourism industry, and has given rise to a series of new employment positions closely related to digital technology, providing broader development space and transformation opportunities for the cultivation of tourism professionals. In this digitalization context, The most urgent thing is to explore the best talent cultivation model to adapt to the changes in the tourism industry environment and the demand for tourism talents. On the basis of drawing on the practical experiences from professional talent cultivation reform in existing universities, combined with the problems in the process of tourism majors` talents cultivation, this paper proposes an innovative plan for the talent cultivation mode of higher vocational tourism majors under the theoretical framework of “job-course-competition-certification” integration. It is hoped that through the vivid practice of three integration scenarios: “job- course integration”, “course-competition integration”, and “course-certification integration”, it will optimize the talent training system for tourism majors in higher vocational colleges, and achieve high-quality and skilled training for tourism majors talents.

Metabolic responses of Euglena gracilis under photoheterotrophic and heterotrophic conditions

The protist Euglena gracilis has various trophic modes including heterotrophy and photoheterotrophy. To investigate how cultivation mode influences metabolic regulation, the chemical composition of cellular metabolites of Euglena gracilis grown under heterotrophic and photoheterotrophic conditions was monitored from the early exponential phase to the mid-stationary phase using two different techniques, i.e, nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HRMS). The combined metabolomics approach allowed an in-depth understanding of the mechanism of photoheterotrophic and heterotrophic growth for biomolecule production. Heterotrophic conditions promoted the production of polar amino and oxygenated compounds such as proteins and polyphenol compounds, especially at the end of the exponential phase while photoheterotrophic cells enhanced the production of organoheterocyclic compounds, carbohydrates, and alkaloids.

Enhancing the biosynthesis of polyunsaturated fatty acids by Rhodotorula mucilaginosa and Lodderomyces elongisporus

Single-cell oils (SCO) produced by oleaginous yeast hold promise as a sustainable alternative for producing nutritionally and pharmaceutically valuable lipids. However, the accumulation of oils varies substantially between yeast spp. Consequently, identifying well-suited producers with a high innate capacity for lipids biosynthesis is paramount. Equally important is optimizing culturing and processing conditions to realize the total lipids production potential of selected strains. The marine Rhodotorula mucilaginosa and Lodderomyces elongisporus yeast were investigated to explore their potential for polyunsaturated fatty acids (PUFAs) production on high glucose media (HGM) using two-stage culture mode. Both strains accumulated > 20% (w/w) of their dry cell weight as lipids when grown on HGM using a two-stage culture system. Both yeast isolates exhibited a maximal lipid/biomass coefficient (YL/X) of 0.58–0.66 mg/mg at 7 °C and 0.49–0.53 mg/mg at 26 °C when grown on 8% glucose and produced monounsaturated and PUFAs similar to that of Menhaden and Salmon marine oils. For the first time, significant amounts of Eicosapentaenoic acid (19%) and Eicosadienoic acid (19.6%) were produced by L. elongisporus and R. mucilaginosa, respectively. Thus, the SCO derived from these wild strains possesses significant potential as a substitute source for the industrial-scale production of long-chain PUFAs, making them a promising contender in the market.Graphical

Exploration of Innovation Ability Cultivation Pattern for Environmental Students based on Open Experiments

The cultivation of innovation ability is especially important for students majoring in environmental science and engineering. In order to solve the problems existing in the process of traditional innovation ability cultivation, a novel pattern of innovation ability cultivation for environmental science and engineering majors based on open experiments is proposed. Through the exploration of platform construction, project declaration, process supervision and effect evaluation, an innovation-oriented open experiment system is constructed and put into practice, which provides students with richer practice opportunities and innovation space, further improves students' practical ability and innovation ability, and is a scientific and effective mode of cultivation of innovation ability.

Developing a safety engineering experimental course using “Macro-Safety” discipline integration

Professional experiment courses are important to students’ understanding and recognition of professional knowledge as links to professional experiments. The University of Science and Technology Beijing’s “Macro-Safety” discipline integration approach to safety engineering was used to develop safety engineering experimental courses. Development methods were discussed. A research background in multiple dominant disciplines helped establish the cultivation mode for innovation in students majoring in safety engineering. We combined the traditional teaching mode with multi-disciplinary, innovative experimental teaching, which improved the students’ enthusiasm for studying safety engineering, enhanced their practicality and innovation, and laid a solid foundation for their comprehensive development. The research results of this paper can be used in metallurgy, materials, and other related majors.

Nitrogen cycling process and application in different prawn culture modes

AbstractNitrogenous waste is a global concern in aquatic ecosystems. In the shrimp farming system, feeding is the main input of nitrogen, which leads to the accumulation of nitrogenous waste, such as ammonia, nitrite, and nitrate. Nitrogen cycling is crucial for nitrogenous waste removal and for the stability of the aquaculture system. Under the action of different functional microorganisms, a variety of nitrogen cycling pathways can be used for the transformation and removal of nitrogenous waste. Understanding the complexity of the nitrogen cycle is necessary for improving the aquaculture environment. This review examines the many components and mechanisms involved in the nitrogen cycle in shrimp farming system, including nitrification, denitrification, anammox, heterotrophic assimilation, and autotrophic assimilation. Because of the difference in aquaculture characteristics, nitrogen cycling pathways in different shrimp culture modes are diverse. The current application of the nitrogen cycle in shrimp farming system, including the outdoor pond mode and indoor industrialized mode, was presented in combination with the requirements for dissolved oxygen (DO), organic matter, carbon–nitrogen ratio, light, and other environmental factors. Overall, nitrification, heterotrophic assimilation, autotrophic assimilation, and heterotrophic denitrification are the main nitrogen cycle processes in the shrimp culture system. According to the characteristics of aquaculture modes and microorganisms, utilizing different nitrogen cycle processes can enhance the efficiency of the nitrogen cycle, facilitate the elimination of nitrogenous waste, optimize the aquaculture water environment, and improve overall aquaculture benefits.

RESEARCH ON THE COLLABORATIVE MODEL OF HIGH-LEVEL SPORTS AND TOURISM TALENT CULTIVATION BETWEEN CHINESE SPORTS UNIVERSITIES AND ENTERPRISES

The integration of the sports and tourism industries has become a major development trend, creating new demands for high-level talent cultivation. However, Chinas current talent training model needs to adapt to the needs of the integrated development of sports and tourism. This study explores the collaborative talent cultivation model between Chinese sports universities and enterprises to promote high-level talent cultivation for the integrated development of sports and tourism. This research analyzes the current status, problems, and influencing factors of collaborative talent cultivation between universities and enterprises. It finds that the lack of clear talent training objectives, insufficient practical training opportunities, and inadequate incentive mechanisms hinder the effectiveness of the collaborative model. Based on this, the study proposes a collaborative talent cultivation mode that clarifies the roles and responsibilities of universities and enterprises, optimizes the curriculum system and practical training links, and establishes a long-term cooperation mechanism. Implementing this collaborative model can effectively integrate the advantages of universities and enterprises, enhance the practical and innovative abilities of high-level sports and tourism talents, and provide solid human resource support for the integrated development of the two industries. The findings of this study have important theoretical and practical significance for improving the talent training system in the field of sports and tourism in China. KEYWORDS: University-Enterprise Collaboration， High-Level Sports and Tourism Talents, Integrated Development

Research on the Development Status of Higher Vocational Education in China

With the rapid development of China's economy and the transformation and upgrading of industrial structure, higher vocational education plays an important role in cultivating high-quality skilled personnel. This paper analyses the current situation of the development of higher vocational education in China, discusses the challenges it faces, and puts forward corresponding countermeasures and suggestions. The study finds that the number and scale of schools in China's higher vocational education have been expanding, and the setting of disciplines and specialisations has been gradually diversified, but there are still certain problems in the construction of teaching staff and the guarantee of teaching quality. Meanwhile, the mismatch between higher vocational education and industrial demand, and the unbalanced distribution of educational resources are also challenges to the current development. In response to these problems, this paper puts forward countermeasures and suggestions such as diversified talent cultivation modes, balanced allocation of educational resources, strengthening industry-university-research-use cooperation, reinforcing the construction of faculty, as well as promoting the optimisation and updating of disciplines and specialties. These measures will help to improve the quality and level of China's higher vocational education and meet the demand for talents in social and economic development.

Intensifying outdoor cultivation of oleaginous microalgae in photobioreactors for simultaneous wastewater bioremediation and low-cost production of renewable biodiesel feedstocks

Oleaginous microalgae are gaining increasing attention for simultaneous wastewater bioremediation and biofuel feedstock production. In this study, four oleaginous species of microalgae including Chlamydomonas sp. WWP, Scenedesmus sp. HP56, Haematococcus sp., and Nannochloropsis sp., were screened for their growth and lipid production at 30, 35, and 40 °C, under light intensity at 3, 4, and 5 klux. Among the strains screened, Haematococcus sp. grew best and gave comparable high growth at 30 °C and 35 °C (0.7–0.8 g/L) indicating its thermotolerant characteristic, and the optimal light intensity was found at 4 klux. Haematococcus sp. also grew well using unsterile secondary effluent from the seafood processing plant and produced high biomass (0.53–0.57 g/L) and lipids (0.17–0.19 g/L), especially in the column photobioreactor (CP). The repeated-batch outdoor cultivation mode showed that CP equipped with four-blade baffles was most efficient for the production of biomass (0.40–0.63 g/L) and lipids (0.15–0.30 g/L) during 4 cycles. The microalga also removed total nitrogen and total phosphorus from secondary effluent by 70.59% and 94.97%, respectively. The fatty acid compositions in microalgal lipids were C16-C18, especially palmitic acid, oleic acid, and linolenic acid, which show superior fuel properties, suggesting their promising use as biodiesel feedstocks. These strategies may have a significant impact not only on bioremediation of industrial effluent but also low-cost production of microalgae-based biodiesel feedstocks.

Innovation of Cultural Education Mode in Agricultural Higher Vocational Schools from the Perspective of Rural Revitalization

In recent years, rural revitalization has become a national strategy, aiming at the problems of unbalanced urban and rural development and lagging rural economic development, aiming at building a socialist modern country in an all-round way. Based on this, the research content of this article mainly focuses on the innovation of cultural education mode in agricultural higher vocational schools from the perspective of rural revitalization. Through in-depth study of the existing cultural education model, combined with the strategic needs of rural revitalization, this article puts forward an innovative cultural education model. Through practical exploration, agricultural higher vocational schools can form a cultural education mode with local characteristics, agricultural culture subject, industrial culture support and social culture assistance. This not only helps to improve students' cultural literacy and vocational skills, but also helps to promote rural revitalization and agricultural development. In addition, this article also puts forward some suggestions for future research to promote the sustainable development and optimization of cultural education mode in agricultural higher vocational schools. In order to provide strong talent support for rural revitalization and promote agricultural higher vocational schools to play a greater role in cultivating outstanding talents.

Physicochemical properties of wild and cultivated Saccharina latissima macroalgae harvested in the Canadian boreal-subarctic transition zone

Saccharina latissima is a brown seaweed used as a food ingredient. The aim of this work was to study possible differences between S. latissima chemical composition, color, mode of cultivation, harvesting period and site and its environmental conditions. Water temperature, salinity, radiation, and fluorescence were monitored in each harvesting site. Chemical composition of S. latissima varied greatly with period and site, with a high content of carbohydrates and ash. Crude protein content varied from 3.7 % to 12.8 %, with a higher concentration observed in wild samples harvested in Bas-St. Laurent (11.1–12.8 %). Cultivated seaweed also presented a high crude protein (12.2 %) and ash (52 % against 27 % in wild samples) concentrations, but crude fiber and carbohydrates concentrations were lower, reaching up to 2.7 and 1.9-fold, respectively, than those in wild seaweeds. S. latissima presented a more intense yellow color in June. A trend of darker and more green-colored seaweeds when cultivated in the end of summer was confirmed. Our results suggest that variations in chemical components and chromaticity of this species are probably affected by complex interactions of environmental conditions.

Study on the matching degree of major groups and industrial groups in higher vocational colleges

Major groups matching industrial groups in higher vocational colleges is the requirement of integration of industry and education. It is a talent cultivation mode based on the demand of regional advantageous industrial groups for talent quality. It is significant to improve the rationality of professional settings in colleges and promote the development of the economy. This study takes the Yi-Jing-Jing Metropolitan area as a case and uses the coupling coordination model to measure the matching degree of the industrial groups and the major groups. The conclusion is as follows: major groups and industrial groups in this region do not match well, and pay insufficient attention to the development of emerging advantageous industries. Some majors have not adapted to the characteristics of the current era and cannot meet the future development of the industry. According to the research results, it is proposed to strengthen policy guidance, based on changes in demand, and adjust the course content and other aspects to promote the matching of major groups and regional advantageous industrial groups.

Research on the Reform of Talent Cultivation Mode of Financial Accounting Majors in LN College Based on New Business: From the Perspective of Industry-Education Integration

In the era of the digital economy, new demands are being placed on financial accounting talent. To cultivate multifaceted talents suitable for the new business education framework, it need to enhance the quality of financial accounting education through industry-education integration. This paper takes LN College as the research object, and adopts literature analysis method, survey research method and case study method to analyze the current situation of training talents of accounting profession in LN College. The findings reveal several issues: outdated talent cultivation goals, an incomplete application-oriented curriculum system, and an inadequate practical teaching systems and so on. Addressing these issues, the paper proposes realigning the talent cultivation goals, optimizing the application-oriented curriculum system, and constructing a comprehensive practical teaching system. These strategies aim to nurture multifaceted financial accounting talents. Furthermore, this study provides references for other majors and colleges.

新时代高校培养好青年的路径探究

Youth is the hope of a country and the future of a nation. Training good young people in the new era is of great significance, which is conducive to improving the quality of young people in colleges and universities, helping young people to take up the historical mission and promote national rejuvenation. Therefore, colleges and universities should take the training of the new era ideal, the new era of responsibility, the new era of hardship and the new era of strugglers as the goal and main content. But there are many problems in the process of training young people. Therefore, colleges and universities should form the cultivation mode of cooperative development, set up a well-structured cultivation mechanism and perfect network training system. The cultivation of good young people in colleges and universities in the new era helps to enhance the sense of historical mission and responsibility of Chinese youth, and plays a significant and far-reaching significance for the future development of the Party and the country and the construction of socialism.