Cultural Strategies Research Articles

Integrated approach for managing groundnut stem rot caused by Sclerotium rolfsii Sacc.

Groundnut (Arachis hypogaea L.), often referred to as the King of oil seeds, is a vital oil seed crop cultivated globally. However, its productivity is hampered by numerous abiotic and biotic stresses, with biotic stresses predominantly due to fungal and bacterial diseases. Among these, soil-borne fungal pathogens cause significant yield losses. Specifically, stem rot disease, caused by Sclerotium rolfsii Sacc., poses a substantial threat, leading to yield losses of up to 80%. This pathogen forms sclerotia, a resilient resting structure that can survive in the soil for many years and germinate under favorable environmental conditions. The persistence of sclerotia and the pathogen's broad host range make managing this disease particularly challenging through a single method. Effective management of stem rot disease necessitates an integrated disease management (IDM) approach, which combines cultural, chemical, and biological strategies. Cultural practices such as crop rotation, deep ploughing, and moisture regulation help to reduce inoculum levels in the soil. Chemical control involves the use of fungicides to reduce the pathogen load in the soil and protect the plants during vulnerable growth stages. Biological control employs antagonistic microorganisms that can inhibit the growth and activity of S. rolfsii. By integrating these diverse strategies, it is possible to effectively manage stem rot disease in groundnut, thereby enhancing productivity and sustainability in groundnut cultivation.

Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production

BackgroundPhaeodactylum tricornutum is a versatile marine microalga renowned for its high-value metabolite production, including omega-3 fatty acids and fucoxanthin, with emerging potential for integrated biorefinery approaches that encompass biofuel and bioproduct generation. Therefore, in this study we aimed to optimize the cultivation conditions for boosting biomass, lipid, and fucoxanthin production in P. tricornutum, focusing on the impacts of different nutrient ratios (nitrogen, phosphorus, silicate), glycerol supplementation, and light regimes.ResultsOptimized medium (− 50%N%, + 50% P, Zero-Si, 2 g glycerol) under low-intensity blue light (100 μmol m⁻2 s⁻1) improved biomass to 1.6 g L⁻1, with lipid productivity reaching 539.25 mg g⁻1, while fucoxanthin increased to 20.44 mg g−1. Total saturated fatty acid (ΣSFA) content in the optimized culture increased approximately 2.4-fold compared to the control F/2 medium. This change in fatty acid composition led to improved biodiesel properties, including a higher cetane number (59.18 vs. 56.04) and lower iodine value (53.96 vs 88.99 g I2/100 g oil). The optimized conditions also altered the biodiesel characteristics, such as kinematic viscosity, cloud point, and higher heating value.ConclusionOur optimization approach reveals the significant potential of P. tricornutum as a versatile microbial platform for biomass, lipid, and fucoxanthin production. The tailored cultivation strategy successfully enhanced biomass and lipid accumulation, with notable improvements in biodiesel properties through strategic nutrient and light regime manipulation. These findings demonstrate the critical role of precise cultivation conditions in optimizing microalgal metabolic performance for biotechnological applications.

Metabolomic Analysis and Computational Biology Reveal the Potential Metabolic Pathways for Improvement of Fucoxanthin and Eicosapentaenoic Acid (EPA) Biosynthesis in Phaeodactylum tricornutum.

This work aims to provide a basis for the enhancement of fucoxanthin (FCX) and eicosapentaenoic acid (EPA) biosynthesis in the microalga Phaeodactylum tricornutum using metabolomics and computational biology. To achieve this, both targeted (UHPLC and GC-FID) and untargeted (FTIR and NMR) analyses were conducted throughout various stages of cell cultivation. Targeted analyses revealed that EPA concentrations peaked at the end of the logarithmic growth phase, while fucoxanthin levels remained consistent from the onset of this phase through to the stationary phase. Untargeted analyses provided metabolic profiles by correlating FTIR absorbance bands with functional groups. When combined with cultivation strategies designed to improve EPA and FCX content, the optimal time for harvesting cells was identified as the end of the logarithmic phase. NMR further highlighted potentially key metabolic pathways for optimizing EPA and FCX production in Phaeodactylum tricornutum, particularly those involved in glyoxylate and dicarboxylate metabolism.

The Cultivation Strategy of College Students' Volunteer Service Spirit

The Cultivation Strategy of College Students' Volunteer Service Spirit

Shaping China's National Image Through Infrastructure: Business Communication Strategies under the Belt and Road Initiative

With the promotion of the Belt and Road Initiative, the global investment and construction activities of Chinese infrastructure enterprises such as China Construction and China Railway have significantly improved the national image and brand awareness. However, in this process, these enterprises face challenges such as cultural differences, information gaps, and local people's doubts about foreign investment. This study explores how Chinese infrastructure companies shape the country's image through effective business communication strategies in the context of the Belt and Road Initiative. Through literature review and case study, this study will focus on the communication strategies adopted by Chinese infrastructure enterprises in countries along the Belt and Road, and how these strategies affect local consumers' perception and trust in Chinese brands. The study reveals that successful communication cases not only enhance brand recognition, but also increase consumer trust in the quality and efficacy of the program. In addition, cultural communication strategies are essential in different markets, helping enterprises integrate into the local society and improve their brand image. This study provides a new perspective and ideas for the brand communication of Chinese infrastructure enterprises in the international market. I contribute theoretical support for future academic research in related fields.

Optimizing Growth Conditions and Biochemical Properties of Chondracanthus acicularis (Rhodophyta) in Laboratory Settings

This study aimed to evaluate the laboratory cultivation of Chondracanthus acicularis, focusing on key environmental parameters such as nutrient levels and light exposure. The results provide insights into the optimal growth conditions and biochemical composition of C. acicularis, which are crucial for its sustainable exploitation in industrial applications. Significant differences in the relative growth rate (RGR) and productivity (Y) were found between the different treatments. Seaweed grown on Provasoli (PES) Medium with white LED light and red LED light showed the best growth rates. Negative growth was observed in treatments with Nutribloom plus®, and blue LED light. The proximate composition analysis revealed a high moisture content across all treatments, with significant differences in ash and organic matter content between the treatments. The use of LED light played a crucial role in optimizing growth by influencing photosynthetic efficiency and pigment production. The proximate composition varied significantly between treatments, especially ash and organic matter. Light and nutrient conditions also influenced pigmentation and colour characteristics, with significant changes in phycoerythrin, phycocyanin, and chlorophyll concentration. PES treatments consistently showed the highest colour variation. These findings highlight the influence of environmental conditions on seaweed growth, productivity, pigmentation, and proximate composition, and provide valuable insights for optimized cultivation strategies.

Developing Language Assessment Literacy of Pre-Service English Teachers: Frameworks and Cultivation Strategies

Assessment is a crucial aspect of the teaching process for teachers. Teachers’ assessment literacy is closely related to students’ learning outcomes. The language assessment literacy of foreign language teachers is a significant component of both teachers’ professional development and students’ learning, and it has become a research hotspot in the field of domestic language testing. Based on clarifying the theoretical framework of language assessment literacy, this paper proposes the main cultivation paths for pre-service English teachers’ language assessment literacy, aiming to provide inspiration and references for the cultivation, reform, and development of teachers in basic foreign language education.

Intergenerational transmission or cultural loss? The continuity of ethnic identity among the Yao minority in China

PurposeThis study aims to examine the intergenerational transmission of ethnic culture among the Yao minority in China, focusing on how rapid societal changes and modernization impact cultural identity maintenance. The research seeks to understand the perspectives of different generations of Yao individuals regarding their traditional culture, external influences and ethnic identity. The study aims to identify factors that facilitate or hinder cultural transmission, assess the effectiveness of current cultural preservation efforts and uncover potential strategies for maintaining ethnic identity in a rapidly changing society. Additionally, the research investigates the role of traditional elements, such as ethnic clothing, in connecting younger generations to their cultural heritage.Design/methodology/approachThis study employed a qualitative approach using grounded theory methodology to explore the intergenerational transmission of Yao ethnic culture in China. In-depth, semi-structured interviews were conducted with 30 Yao individuals representing different generations. Participants were purposively selected from two Yao villages in southern China. Data collection also included researcher observations and memos. Interviews were conducted in Chinese, translated to English and analyzed using NVivo12 software. The analysis followed the grounded theory approach of open, axial and selective coding. Triangulation was achieved through discussions with participants' peers and family members. Ethical considerations included obtaining informed consent, ensuring participant anonymity and securing institutional review board approval.FindingsThe study revealed three distinct generational attitudes towards Yao culture: rejection among older generations, semi-openness in middle-aged groups and initiated closeness in younger generations. Despite government policies supporting population growth, ethnic identity is not effectively transmitted across generations. Economic disparities between Yao communities and external society significantly influence cultural preservation, with younger generations prioritizing economic opportunities over traditional practices. However, traditional Yao clothing emerged as a potential link between younger individuals and their cultural heritage, offering a promising avenue for cultural preservation. The research also highlighted the impact of education, technology and media on shaping attitudes towards traditional culture and external influences across different age groups.Originality/valueThis study provides insights into the cultural transmission within the Yao minority in China, a previously understudied ethnic group. It uniquely examines the interplay between economic development, government policies and intergenerational attitudes in shaping ethnic identity maintenance. The research reveals an unexpected potential for traditional clothing to serve as a bridge between younger generations and their cultural heritage, offering a fresh perspective on cultural preservation strategies. By identifying distinct generational attitudes towards cultural identity, this study contributes valuable knowledge to the fields of ethnic studies, cultural anthropology and social psychology, while also informing policymaking for minority cultural preservation in rapidly modernizing societies.

Role of Cultural Graphics Strategies in Shaping Urban Identity: A Quantitative Study of User Perception in Qinzhou City

The paper attempts to investigate the role that street art, murals, and other signage play in forming and defining urban identity through research on Qinzhou City, Guangxi, China. The study employs a quantitative approach and structural equation modeling (SEM-PLS), which examines the interaction among cultural identity, social identity, media consumption, personal values, and beliefs and how these interact to affect cultural perception. Results of the study indicate that both cultural identity and social identity have significant influences on cultural perception, with media consumption and personal values and beliefs acting as critical mediators. Cultural Identity fosters the connection of personal beliefs strongly, whereas Social Identity tends to drive media consumption behaviors that affect cultural understanding. The study provides theoretical and practical implications for urban planners, policymakers, and media strategists in exploiting cultural graphics to improve urban cultural environments. Despite its limitations in geographical scope, the study provides useful insights into the intersection of cultural and social dynamics and offers a model for urban cultural policy and development strategies.

Best Practices in Cybersecurity Training: Enhancing Employee Awareness to Mitigate Risks

This technical article explores the critical role of cybersecurity training programs in addressing the growing challenges of cyber threats in modern organizations. The article examines how human factors contribute to security vulnerabilities while also presenting opportunities for strengthening organizational defense through comprehensive training initiatives. Through this detailed article of current research and industry reports, this article investigates the implementation of structured training approaches, measurement frameworks, and cultural transformation strategies. The article encompasses key aspects including baseline assessments, multi-tiered training architectures, performance metrics, security-conscious culture development, technical integration through learning management systems, compliance documentation, and future-proofing methodologies. The article demonstrates that organizations implementing comprehensive security awareness programs experience significant improvements in threat detection, incident response, and overall security posture while highlighting the importance of leadership engagement and continuous adaptation in maintaining effective cybersecurity training programs.

Effects of Climatic, Chemical, and Cultural Control Strategies on Community Composition of Auchenorrhyncha and Population Dynamics of Two Major Green Leafhopper Pests in Peach Orchards

A three-year study in two Mediterranean peach orchards monitored Auchenorrhyncha species, including the main vectors of plant pathogens causing significant economic losses. The research focused on the management of two polyphagous leafhoppers, Asymmetrasca decedens and Hebata (Signatasca) solani. Yellow sticky traps were used to track species dynamics and assess population trends in relation to meteorological factors such as temperature, rainfall, and humidity. Chemical treatments and early green pruning were evaluated for pest control effectiveness. Fifty-five Auchenorrhyncha species were identified, including vectors of Xylella fastidiosa. H. solani appeared from spring to early summer, and was gradually replaced by A. decedens, which peaked in late summer. Higher temperatures favoured A. decedens, while rainy days significantly reduced its presence. H. solani responded less consistently to meteorological factors, but increased with higher minimum relative humidity, and decreased with higher total humidity. These results highlight the need for targeted chemical control strategies to manage A. decedens during its peak season and the benefits of early green pruning to protect susceptible trees in spring. The results emphasise the role of climate in pest dynamics and provide important insights for IPM programmes to reduce economic losses in Mediterranean peach orchards worldwide.

Underscoring long-term host-microbiome interactions in a physiologically relevant gingival tissue model

The human body houses many distinct and interconnecting microbial populations with long-lasting systemic effects, where the oral cavity serves as a pathogens’ reservoir. The correlation of different disease states strongly supports the need to understand the interplay between the oral tissue niche and microbiome. Despite efforts, the recapitulation of gingival architecture and physiological characteristics of the periodontal niche has yet to be accomplished by traditional cultural strategies. Here, we are showing for the first time the investigation of host–microbiome interactions in healthy conditions within a human oral tissue model over seven days. Our results indicated long-term host and microbiome viability, host barrier integrity, phenotypic functional response, and preservation of healthy microbial populations and interbacterial dialogs. This in vitro platform can maintain tissue homeostasis at the interface of the periodontal niche, thus, offering opportunities to identify predictive disease biomarkers and to develop intervention strategies to promote oral and overall health.

Navigating implementation strategies: insights from XPS programmes

PurposeThis study explores how industrial production companies adjust implementation strategies to deliver value with their company-specific production system (XPS) in a multi-site setting. Implementing an XPS is part of operations management and is supposed to mobilise employees resource-efficiently to increase operational performance.Design/methodology/approachBased on a multiple-case study, this study qualitatively explores the patterns of implementation decisions within and between five multi-site production companies. Conclusions were drawn on a mix of timely and retrospective data gathered through individual interviews with senior managers and joint workshops where they interacted and shared experiences.FindingsThis study identifies the fact that companies respond differently to non-unique implementation challenges, constituting various decision patterns when implementing their XPS. This paper identifies four implementation strategies (Education, Tool, Pragmatic and Culture Strategy) that explain various implementation setups. More experienced companies frequently shifted between strategies to return to previously de-prioritised implementation aspects.Originality/valueThis paper offers a dynamic model for XPS implementation in contrast to a simplified tool–system–culture sequence in literature today. Companies that deliberately shift implementation strategy by understanding the benefits and challenges of their decisions are more likely to keep momentum in the XPS implementation.

Research on the Strategy of Tea Culture Promoting the Integrated Development of Tea Culture and Tourism under the Revitalization Strategy —Taking Guiping Xishan Tea as an Example

Research on the Strategy of Tea Culture Promoting the Integrated Development of Tea Culture and Tourism under the Revitalization Strategy —Taking Guiping Xishan Tea as an Example

Research on the Regional Dissemination Paths of Chinese Culture and Cultural Diplomacy Strategies in the Context of the "Belt and Road Initiative"

Research on the Regional Dissemination Paths of Chinese Culture and Cultural Diplomacy Strategies in the Context of the "Belt and Road Initiative"

Development of scalable cultivation strategy for microalgal biomass generation using wastewater and integrated biorefinery approach for biofuel production

Development of scalable cultivation strategy for microalgal biomass generation using wastewater and integrated biorefinery approach for biofuel production

Diversidade genética das características de processamento do café em Coffea canephora

ABSTRACT: With advancements in coffee cultivation, several traits may be considered in selection of plants, which must exhibit a set of favorable characteristics. The outturn index, defined as the relationship between the mass of mature fruit and processed beans, emerges as a key factor influencing productivity. This study characterized the outturn over two harvests of 57 clones marketed in the public domain and 10 registered cultivars. The analysis considers the effects of genotypes, measurements, and genetic progress achieved through plant selection. According to the maturation cycle of each clone, washed samples of cherry coffee were collected considering a completely randomized factorial design for characterization of the effects of genotypes, years, and the genotype × years (GY) interaction. The drying, peeling, and the outturn index were individually assessed. Despite the significant effects of the GY interaction, genotypes demonstrated minimal alterations in their ranking across measurements. Clones with higher outturn, including LB30, BRS1216, LB12, N7, LB10, LB20, BRS3220, and AS5, exhibited an average outturn of 25.51%, reflecting a gain of 12.17%. Conversely, clones with lower outturn, such as BG180, GJ30, GJ20, AS7, AS10, P42, N1, and P60, had an average of 19.15%, indicating a reduction of 14.02% compared to the general mean. Analyzing the distribution of the outturn values, 19.4%, 62.7%, and 17.9% of genotypes were classified as high, medium, and low outturn, respectively, providing valuable insights for optimal cultivation strategies.

Enhanced carbon dioxide bio-fixation and protein production of Chlorella pyrenoidosa through a highly efficient sequential heterotrophic and photoautotrophic culture strategy

Chlorella pyrenoidosa is one of the most promising microalgae for protein production with carbon dioxide (CO2) bio-fixation. However, the high production cost and low production capability under traditional photoautotrophic culture mode had severely limited its application. Here, a highly efficient sequential heterotrophic and photoautotrophic cultivation strategy was established. Firstly, the heterotrophic seeds culture conditions were optimized with glucose as the carbon source and 1 g L−1 of yeast extract was selected as a useful stimulus to boost cell growth. Then, the heterotrophic cells were transferred to photoautotrophic conditions directly and cultured under varied concentrations of CO2. Moreover, 8 % (v/v) of CO2, high light and high initial cell density were found to be beneficial for cell growth and CO2 sequestration. Finally, different initial nitrogen concentrations and nitrogen feeding strategies were evaluated to increase the protein content to 62.69 % of dry biomass. These results provide feasible strategies for efficient CO2 sequestration and protein production jointly in C. pyrenoidosa.

Effects of hormones on the growth and metabolite production of Amphidinium carterae under carbon sufficient and carbon limited conditions

The dinoflagellate Amphidinium carterae produces valuable fatty acids, pigments, and bioactive polyketides (e.g., amphidinols). Despite recent advances in its intensive cultivation, challenges remain that justify further work. This study explored the effects of phytohormone supplementation on growth and metabolism using a One Strain Many Compounds (OSMAC) approach. Auxins, cytokinins, jasmonates, amines, abscisic acid, gibberellic acid, and other signaling molecules were screened in batch cultures. Synthetic auxin 2-naphthoxyacetic acid (BNOA) at a final concentration of 14.84 μM (3 mg/L) emerged as the most stimulant. On a small scale, BNOA increased biomass productivity by up to 32 % and hemolytic activity in terms of productivity by 50 %. Scaling up in 10 L bubble column photobioreactor cultures with the addition of BNOA, but without CO2 supplementation, resulted in levels of amphidinol production comparable to those achieved with CO2 addition. Compared to the control without BNOA, BNOA cultures generated 2.2-fold more biomass during the stationary growth phase, and amphidinol production increased fourfold per unit of biomass. This work reveals that the addition of exogenous phytohormones such as BNOA can sustain the production of valuable metabolites in A. carterae, eliminating the need for additional CO2 and its associated costs. This promising approach highlights the potential of hormone supplementation as a cost-effective and efficient cultivation strategy for dinoflagellate bioprocesses.

The Integration Strategy of College English Teaching and Traditional Culture under the Background of Informationization

The Integration Strategy of College English Teaching and Traditional Culture under the Background of Informationization