Culturable Fungi Research Articles

Cultivable Fungi from Amazon River Dolphins Engaged in Wildlife Ecotourism in the Anavilhanas National Park, Brazil.

Amazon River dolphins are an important flagship species in the Anavilhanas National Park, Brazil, where they interact with visitors. This study aimed to quantify and identify fungi isolated from dolphin skin and oral samples and their surrounding environment in this unique ecosystem. Samples were collected from three dolphins and water samples from Flutuante dos Botos and the Novo Airão city harbor. Fungi were isolated using culture media and identified through micromorphology assays and ITS region sequencing. Oral swab samples resulted in culture of Trichosporon montevideense and Exophiala dermatitidis. Skin samples from one dolphin revealed Toxicocladosporium irritans and Diaporthe lithocarpus. Water samples exhibited higher fungal counts and diversity, with Rhodotorula mucilaginosa, Exophiala dermatitidis, Penicillium citrinum, Fomitopsis meliae, and Nectria pseudotrichia identified at the collection site and Candida spencermartinsiae and Penicillium chermesinum at the city harbor. This study provides important insights into the fungal diversity associated with Amazon River dolphins and their environment, enhancing our understanding of the public health and ecological dynamics in the Anavilhanas National Park.

Study on the use of Imazalil to continuous cropping obstacle of Ganoderma lucidum caused by Xylogone ganodermophthora

BackgroundThe Xylogone ganodermophthora is a pathogenic bacterium that poses a significant challenge to the continuous cultivation of the Ganoderma lucidum fungus. This study aims to identify and investigate specific agents for the effective prevention and control of X. ganodermophthora, establishing a theoretical foundation for overcoming this persistent challenge in G. lucidum cultivation.ResultsUsing different G. lucidum soil as materials to study the presence of X. ganodermophthora in the soil. Additionally, the plate confrontation test was employed to investigate the impact of X. ganodermophthora on G. lucidum growth. The impact of physical factors and antibacterial agents on pathogenic bacteria was successfully carried out, with a further exploration of the effectiveness of field control. PCR amplification experiment and sequencing analysis verified that X. ganodermophthora existed in G. lucidum continuous cropping obstacle soil. This pathogenic bacteria has a significant inhibitory effect on the growth of G. lucidum, with an inhibition rate of up to 52.23%. High temperature, low temperature, light and other physical factors have no obvious inhibitory effect on this pathogen. Further investigation revealed that specific drugs, such as low concentrations (10 μL/mL) of Acticide DB20 and Imazalil, could effectively inhibit X. ganodermophthora growth in G. lucidum. Among them, Imazalil has a notable inhibitory effect on the growth of X. ganodermophthora.ConclusionsIndoor toxicity test and field control results showed that Imazalil could effectively control the growth of pathogen X. ganodermophthora in G. lucidum continuous cropping obstacle, and promote the growth of G. lucidum.Graphical

Seasonal discrepancy of airborne fungal diversity and community structure in Lentinula edodes factory

ABSTRACT Understanding the composition and community of airborne mycobiota associated with fungal diseases was critical for mushroom production and disease control. In this study, we first investigated airborne fungi concentrations in different function areas of a Chinese shiitake factory. Then, high-throughput sequencing was employed to characterise the airborne fungal compositions and communities of this factory during high and low incidence seasons of shiitake artificial log rot disease (SLRD). The lowest concentration of culturable airborne fungi was observed in spawn-running greenhouse (250–450 CFU/m3), while the airborne fungi concentrations in cultivation greenhouses were up to 7,000–8,500 CFU/m3. The airborne fungal communities across distinct function areas showed convergence. The four major mushroom contaminants, Trichoderma, Penicillium, Cladosporium, and Aspergillus were the stable core fungi. Seasonal shifts in compositions, abundances, and communities of airborne fungi were systematically analysed and found to be associated with SLRD. Compared with the SLRD low incidence season, the airborne fungal communities were rich but uneven in the SLRD high incidence season, in which Trichoderma was dominant. Further analysis indicated that the favourable temperature and humidity caused the proliferation of Trichoderma and then affected the fungal communities, which presented an SLRD-seasonal variation. SLRD incidence could be significantly reduced by reasonably increasing the ambient temperature. For the first time, we comprehensively characterised the dynamics of airborne fungal communities across the SLRD occurrence in the shiitake factory. Findings here also provided valuable information for fungal exposures in the mushroom industry.

Anaerobic Digestion as a Possible Method of Managing Waste from Mushroom Production with Sewage Sludge as Co-Substrate

The mushroom agroindustry generates a huge amount of waste from mushroom production (WMP). The composition of WMP is not standardized but differs mainly in terms of organic matter (OM) content and OM biodegradability. This makes WMP management, including anaerobic digestion (AD), a significant challenge. A potential solution could be co-digestion of WMP with municipal sewage sludge (SS), especially SS generated in small rural wastewater treatment plants (WWTPs). Therefore, this study investigated mesophilic methane production (MP) from WMP, SS, and mixtures of SS and WMP at ratios of 70:30, 50:50, and 30:70 (w/w OM). Even though the maximum cumulative MP from WMP was relatively low (approx. 60 NL/kg OM), co-digesting WMP with SS increased both MP and the methane content of the biogas: with 30%, 50%, and 70% shares of SS, MP increased almost 2, 2.5, and 3.3 times, and the methane content increased to 61%, 62%, and 64%, respectively. As the SS content was increased, the kinetic coefficients of MP and OM removal decreased (from 0.211 to 0.146 d−1 and from 0.215 to 0.152 d−1), whereas the initial rate of MP and of OM removal increased (from 12.5 to 36.8 NL/(kg OM·d) and from 0.51 kg OM/(m3·d) to 0.59 kg OM/(m3·d), respectively). The effectiveness of OM removal (EOMrem) was lowest with WMP only, at 46.6%. When the SS content of the mixtures was increased to 30%, 50%, and 70%, EOMrem also increased to 55.3%, 60.1%, and 64.9%, respectively. The relationship between maximal MP and the overall OM removed was such that both increased simultaneously. The higher values of EOMrem and, consequently, the lower final contents of OM with more effective MP indicate that the organics were degraded more efficiently. These results suggest that co-digestion may be a profitable solution for simultaneously utilizing both of these waste products, increasing the efficiency of biogas production to such an extent that it would be profitable to conduct AD on mushroom farms. This is a flexible approach that allows varying proportions of WMP and SS to be used, depending on the availability of both substrates and the energy needs of the mushroom farm. However, it should be borne in mind that a higher share of WMP results in lower gas productivity.

Bioaerosol Exposure during Sorting of Municipal Solid, Commercial and Industrial Waste: Concentration Levels, Size Distribution, and Biodiversity of Airborne Fungal

A study was carried out in a waste sorting plant (WSP) located in France, treating dry recyclable household waste (DRHW) as well as dry recyclable commercial and industrial waste (DRCIW). Stationary and personal inhalable samples were collected in the WSP in order to investigate bioaerosols (sampling on a filter; 2 L/min and 10 L/min) and airborne dust (CIP; 10 L/min). The aim of the study was to assess the extent to which the measurement of concentration, species composition, and particle size distribution contributes to a better assessment of the biological risks associated with exposure. The results confirmed that waste and waste sorting activities are sources of airborne fungi. Indeed, ambient concentrations ranged from 7.3 × 103 to 8.5 × 105 colony-forming units (CFU)/m3 for culturable fungi and up to 4 mg/m3 for dust. Personal exposure to inhalable dust was found up to 3 mg/m3 for dust and ranged from 8.6 × 103 to 1.5 × 106 CFU/m3 for fungi. Airborne fungal communities were found to be dominated by the Penicillium genera in both bioaerosols and settled dust samples, followed by the Aspergillus, Cladosporium, Wallemia, Mucor, and Rhizopus genera. Fungi were carried by particles of aerodynamic diameters, mainly between around 2.0 and 10.0 µm. The findings dealing with size distribution and biodiversity of bioaerosols suggest that employees are exposed to complex bioaerosols during their work and help to make a finer diagnosis of the risks involved, which is often difficult in the absence of any occupational exposure limit (OEL) value for bioaerosols in general.

Modification of the existing maximum residue levels for fluxapyroxad in kaki/Japanese persimmons and cultivated mushrooms.

In accordance with Article 6 of Regulation (EC) No 396/2005, the applicant BASF SE submitted two requests to the competent national authority in Spain and Ireland to modify the existing maximum residue levels (MRLs) for the active substance fluxapyroxad in kaki/Japanese persimmons and in cultivated fungi, respectively. The data submitted in support of the requests were found to be sufficient to derive MRL proposals for kaki/Japanese persimmons and cultivated fungi. Adequate analytical methods for enforcement are available to control the residues of fluxapyroxad on the commodities under consideration at the validated LOQ of 0.01 mg/kg. Based on the risk assessment results, EFSA concluded that the short-term and long-term intake of residues resulting from the use of fluxapyroxad, according to the reported agricultural practices, is unlikely to present a risk to consumer health. The long-term consumer risk assessment is indicative, pending the submission of the confirmatory data requested under the MRL review.

Precision Mushroom Farm Monitoring System With Classification of Toxic Mushrooms in Punjab : A Comprehensive Review

Precision Mushroom Farm Monitoring System With Classification of Toxic Mushrooms in Punjab : A Comprehensive Review

Utilizing Analytical Hierarchy Process for Evaluating the Performance of Straw Mushroom (Volvariella spp.) Farmers in North-Eastern Region of Karawang, West Java, Indonesia

Producers of Straw Mushroom (Volvariella spp.) in the North-Eastern region of Karawang, West Java, Indonesia predominantly operate within the sub-districts of Cilamaya Kulon, Cilamaya Wetan, and Banyusari. A noticeable decline in Straw Mushroom cultivation has been observed in this region, which can be ascribed to various factors including suboptimal farming practices, diminished seed quality, and erratic weather conditions impacting production outcomes. It is imperative to enhance the efficiency of mushroom farming across multiple stages encompassing planning, procurement of raw materials, processing, Maintenance, harvesting, marketing, shipping, handling returns, and ensuring consumer satisfaction. This study aims to evaluate the productivity of mushroom farmers, with a specific focus on the top-performing farmers in the North-Eastern region of Karawang. Proportionate stratified random sampling was employed as the sampling technique, and data were collected through structured interviews utilizing a questionnaire. Quantitative analysis, employing the Analytical Hierarchy Process method, was utilized for data analysis. The findings reveal that planning criteria hold the highest priority value of 0.306, whereas satisfaction criteria have the lowest priority value of 0.018. The top-performing farmers achieved a score of 2.08, while the lowest performing farmers attained a score of 1.10

Turning Trash into Treasure: Utilizing Sericultural Wastes in Mushroom Cultivation

Sericulture, an agro- based industry, generates substantial biodegradable waste, which, when repurposed, can significantly enhance mushroom production. This study explores the innovative transformation of sericultural waste into nutrient- rich substrates for mushroom cultivation, addressing global food insecurity and waste management challenges. The study also highlights the versatility of mushrooms, particularly oyster mushrooms (Pleurotus spp.), in utilizing diverse lignocellulosic wastes. It delves into the nutritional superiority of sericultural by-products over conventional substrates and their potential to reduce environmental pollution. The review underscores the economic and ecological benefits of integrating sericultural waste into mushroom cultivation, contributing to sustainable agriculture and a circular economy. Future directions emphasize the need for optimizing substrate composition and further research to maximize the yield and quality of mushrooms cultivation on sericultural waste, paving the way for a sustainable and profitable synergy between sericulture and mushroom farming.

Diversity of Soil-Borne Fungi Isolated from Places Frequently Visited by People in the City of Wrocław (Poland)

In this study, we identified culturable microscopic fungi in soil from areas frequented by people (parks, allotments, and other green areas) in the city of Wrocław (Poland). In addition to general species analysis, attention was focused on keratin-degrading fungi. From 60 soil samples (12 study sites), we obtained 75 isolates of keratinophilic and keratinolytic fungi using the hair bait method, and 54 isolates of fungi were isolated on PDA (Potato Dextrose Agar) medium. Based on morphological and molecular analyses, a total of 37 strains were identified, classified into 2 phyla, 11 families, 17 genera, and 30 filamentous species. The mean values of the Shannon Diversity Index for both experimental variants ranged from 0.074 to 0.117. The most common species was the Penicillium genus, which accounted for 33.33% of all fungal species obtained in these studies. These fungi are common in both indoor and outdoor environments. However, particularly noteworthy in this study are the species belonging to the group of dermatophytes (Arthroderma uncinatum, Keratinophyton wagnerii, Nannizzia gypsea, and Paraphyton cookei), which may pose a real biological threat to humans and animals due to their well-known potential to cause dermatomycosis.

표고버섯 농가 부산물 폐배지 기반 바이오차의 이산화탄소 흡착 연구

표고버섯 농가 부산물 폐배지 기반 바이오차의 이산화탄소 흡착 연구

Endophytic fungus from Gynura japonica: phytochemical screening, biological activities, and characterisation of its bioactive compound

The research aims to identify the chemical constituents of endophytic fungi associated with Gynura japonica and their biological activities. Two endophytic fungi, labelled as GS-1 and GS-2, have been isolated from the leaves of G. japonica. They were cultivated on white rice media for their optimum cultivation time. Cultivated fungi were extracted with ethyl acetate and concentrated to give a crude extract. All crude extracts were evaluated for their phytochemical screening and assayed for their antibacterial and antioxidant activities. Based on the results, fungal GS-1 was the most potential fungus to produce bioactive secondary metabolites and identified as Dimorphiseta acuta. A bioactive compound was isolated from crude extract of fungal D. acuta and identified as emodin. To the best of our knowledge, the study of secondary metabolite and its biological activity of endophytic fungus colonised with the leaves of G. japonica is reported here for the first time.

Design and implementation of a cost-aware and smart oyster mushroom cultivation system

Mushrooms are a nutritious food source, which can play a crucial role in providing affordable sources of proteins, vitamins and minerals for people worldwide, but their cultivation requires extensive training and considerable relevant expertise in order to fine-tune multiple environmental parameters.Internally displaced people in the Northern regions of Syria rely on very small-scale traditional oyster mushroom production, which cannot meet their local demand. Many international and local non-governmental organizations (NGOs) working for Syrian refugees, work on mushroom cultivation projects. They have reported significant difficulties and challenges in mushroom cultivation amongst the targeted beneficiaries. Therefore, the two main questions driving this research are: (1) How can organic mushroom cultivation be promoted using a robust and affordable intelligent mushroom farming system? (2) How can organic mushroom farming practices be simplified to support internally displaced and refugee Syrians?This research evaluates the process of automating mushroom cultivation by designing and implementing a smart oyster (Pleurotus ostreatus) mushroom farming system to remotely monitor and manage environmental parameters, such as temperature, humidity, air quality and illumination, inside the farm. Furthermore, ready and dedicated user-friendly web interfaces were also implemented to enable farmers to remotely monitor and manage their farms through the Internet. As a result, a dependable and cost-effective intelligent oyster mushroom cultivation system was designed and implemented in this work. The system includes remote monitoring and management via user-friendly interfaces. This simplifies mushroom cultivation for not only refugees and displaced communities, but also for mushroom farmers in low-income countries. This work can contribute to the eradication of poverty and hunger, in line with the United Nations Sustainable Development Goals one and two.

Diversity and enzymatic, biosurfactant and phytotoxic activities of culturable Ascomycota fungi present in marine sediments obtained near the South Shetland Islands, maritime Antarctica.

We studied the culturable fungal community recovered from deep marine sediments in themaritime Antarctic, and assessed their capabilities to produce exoenzymes, emulsifiers and metabolites with phytotoxic activity. Sixty-eight Ascomycota fungal isolates were recovered and identified. The most abundant taxon recovered was the yeast Meyerozyma guilliermondii, followed by the filamentous fungi Penicillium chrysogenum, P. cf. palitans, Pseudeurotium cf. bakeri, Thelebolus balaustiformis, Antarctomyces psychrotrophicus and Cladosporium sp. Diversity indices displayed low values overall, with the highest values obtained at shallow depth, decreasing to the deepest location sampled. Only M. guilliermondii and P. cf. palitans were detected in the sediments at all depths sampled, and were the most abundanttaxa at all sample sites. The most abundant enzymes detected were proteases, followed by invertases, cellulases, lipases, carrageenases, agarases, pectinases and esterases. Four isolates showed good biosurfactant activity, particularly the endemic species A. psychrotrophicus. Twenty-four isolates of P. cf. palitans displayed strong phytotoxic activities against the models Lactuca sativa and Allium schoenoprasum. The cultivable fungi recovered demonstrated good biosynthetic activity in the production of hydrolytic exoenzymes, biosurfactant molecules and metabolites with phytotoxic activity, reinforcing the importance of documenting the taxonomic, ecological and biotechnological properties of fungi present in deep oceanic sediments of the Southern Ocean.

How host species and body part determine the microbial communities of five ambrosia beetle species.

The ambrosia beetles are farming insects that feed mainly on their cultivated fungi, which in some occasions are pathogens from forest and fruit trees. We used a culture-independent approach based on 16S and 18S rRNA gene metabarcoding analysis to investigate the diversity and composition of the bacterial and fungal communities associated with five ambrosia beetle species: four species native to America (Monarthrum dimidiatum, Dryocoetoides capucinus, Euwallacea discretus, Corthylus consimilis) and an introduced species (Xylosandrus morigerus). For the bacterial community, the beetle species hosted a broad diversity with 1,579 amplicon sequence variants (ASVs) and 66 genera, while for the fungal community they hosted 288 ASVs and 39 genera. Some microbial groups dominated the community within a host species or a body part (Wolbachia in the head-thorax of E. discretus; Ambrosiella in the head-thorax and abdomen of X. morigerus). The taxonomic composition and structure of the microbial communities appeared to differ between beetle species; this was supported by beta-diversity analysis, which indicated that bacterial and fungal communities were clustered mainly by host species. This study characterizes for the first time the microbial communities associated with unexplored ambrosia beetle species, as well as the factors that affect the composition and taxonomic diversity per se, contributing to the knowledge of the ambrosia beetle system.

Compost Filling Methods Affect Green Mold Disease Incidence in Commercial Mushrooms.

Green mold (Trichoderma aggressivum) is an invasive disease of commercial mushrooms introduced into the United States from Europe that now has spread to commercial mushrooms throughout North America. We examined potential sources of invasive green mold inoculum and the association with different compost filling technologies on a large actively producing commercial mushroom farm. Green mold foci were sampled bed by bed, which generated 20,906 data points. Logistic regression was used to determine treatment differences. Mechanical filling of compost into the beds reduced green mold incidence over hand filling, apparently due to the reduced incidence of worker contact with the floor and between beds. Lower growing beds located closer to the floor had a higher incidence of green mold for both mechanical and hand-filled beds. We conclude that mechanical filling and generally reducing contact with the floor and between beds will reduce spread of green mold in commercial mushroom production.

First Report on Wild Occurrences of Phoenix Mushroom (<i>Pleurotus pulmonarius</i> Fr. Quél.) in Indonesia

The genus Pleurotus is known as a commercially important mushroom and one of the most well-known cultivated mushrooms worldwide. Of many species of Pleurotus, the phoenix mushroom (P. pulmonarius) is cultivated in many countries, including Indonesia. In Indonesia, the farmers and larger companies usually use commercial strains of phoenix mushroom which they purchased from other countries. To date, there was no prior information regarding wild occurrences of P. pulmonarius in Indonesia. During our regular mushroom hunting in Sukabumi, West Java, Indonesia, some edible wild fruiting bodies of light brown Pleurotus were collected. The current study aimed to determine the taxonomical position of our specimens based on morphological and molecular evidence. The combination of morphological and molecular analysis confirmed our specimens as P. pulnonarius. Morphologically, our specimens were distinguished by the small to medium sized fruiting bodies, pileus light brown, pinkish brown, to pale brown, flabelliform in the beginning to expanding broadly ovoid in maturity, lamellae shortly to deeply decurrent, stipe fleshy, eccentric to lateral, concolorous with lamellae, Basidiospores cylindrical to ellipsoid, basidia clavate to club shaped, basidioles are abundant, oleiferous hyphae common. The BLAST result revealed that our specimens posed a high similarity to P. pulnonarius from several countries as the top hits. The ITS phylogenetic tree placed Pleurotus FIPIA-DEP51 in the same clade of P. pulnonarius with 100% BS value. This study reports for the first time the wild occurences of P. pulmonarius in Indonesia. Future study should be done to characterize the cultures of reported mushroom which can potentially be the local strain for cultivation of P. pulmonarius industry in Indonesia.

Health risk assessment and characterization of PM2.5 bound bioaerosols at the municipal solid waste landfill site of Nagpur, India

Landfill being the reservoir of organic waste, serves as the most important source in the generation and emission of bioaerosols in the environment, and these bioaerosols have a significant impact on the environment and public health. In India, there are limited studies on the characterization of culturable bacteria and fungi in the bioaerosols originating from landfills. Therefore, the present study aimed to quantify and identify the bioaerosols and study their characteristics at a landfill site in Nagpur, India. The study describes the seasonal variation of PM2.5, PM2.5-associated bacteria and fungi in the landfill air, their molecular identification, antibiotic resistance, pathogenicity, dispersion and health risk assessment during winter and pre-monsoon seasons at the landfill site and surrounding residential sites in upwind and downwind directions. The study showed PM2.5 concentration was highest in the winter season (265.56 μg/m3) followed by the pre-monsoon season (173.1 μg/m3) at the landfill site. The culturable bioaerosol level was also significantly higher in winter (11056 CFU/m3) in comparison to pre-monsoon season (2244 CFU/m3) at the landfill site. Upon isolating and characterizing the microorganisms in the bioaerosols, some of the bacterial isolates, like Bacillus, Staphylococcus gallinarum, and Streptomyces speibonae were found to be resistant to chloramphenicol, netillin, nitrofurantoin, streptomycin etc. About 30% of the bacterial isolates were found to show β-hemolytic virulence activity. This indicated the presence of multi-drug resistant and potentially pathogenic bacteria in the bioaerosols over the landfill. The health risk assessment in adults indicated that the workers at the landfill site were at risk of bacterial aerosol in the winter season (hazard quotient i.e., HQ>1). The impact of bioaerosols on children needs to be investigated in the further studies and regular monitoring of bioaerosols is suggested to avoid any morbidity. To the best of our knowledge, this is the first study on the characterization of microorganisms from the bioaerosols over an open landfill site in India.

Ambrosia beetles adjust dispersal preferences to their degree of exhaustion

Abstract Delayed dispersal of sexually mature offspring is a fundamental component of cooperative breeding in fungus farming ambrosia beetles. These beetles dwell in logs of recently deceased trees, where female offspring postpone reproduction and invest in alloparental care before dispersing to found a new nest. The wood quality affects investment of adult offspring in alloparental care and hence the breeder's reproductive success. This raises the question of whether dispersing breeders choose habitats strategically to optimise breeding prospects and modulate their offspring's helping propensity. To answer this question, here we tested experimentally whether during dispersal, Xyleborinus saxesenii Ratzeburg (Coleoptera: Scolytinae) uses olfactory cues emitted by mutualistic or pathogenic fungi as an information source on prospective habitat quality. As the mutualistic relationship between the fungi and beetles is obligate, fungus scent should provide a reliable source of information on habitat quality, indicating prior successful colonisation by conspecifics. We found no evidence for direct pathogen avoidance. However, the beetles clearly responded to scent of their mutualistic fungi, dependent on dispersal state. At the onset of dispersal, females avoided the scent of fungus mutualists, whereas they were attracted to them after being exhausted by an experimentally prolonged dispersal time. The observed change of preference during dispersal suggests that beetles freshly emerging from their home gallery may choose pristine habitat by avoiding mutualist scent. This might help to avoid competition with conspecifics and potential pathogen and parasite cross contamination. Exhausted beetles, however, may accept these risks and choose successfully colonised habitat to avoid starvation. State‐dependent adjustment of habitat preferences may be exhibited also in other economically impactful bark and ambrosia beetles, which prompts further study of the olfactory cues used by these beetles during dispersal.

Diversity and Distribution Patterns of Culturable Airborne Fungi in Jiroft City

Background and Aims: Fungal spores constitute a significant portion of primary biological aerosol particles, and large amounts have been identified in the air. They are present in the atmosphere throughout the year, and their concentration varies due to meteorological factors such as temperature, relative humidity, location, construction conditions, and vegetation. Exposure to airborne fungi has been linked with diseases such as allergic respiratory symptoms, rhinitis, asthma, and various infections. This study investigates the diversity and distribution of airborne fungal spores in Jiroft City, located in the southern region of Iran. Materials and Methods: Airborne fungal composition was sampled from indoor, outdoor, and hospital air using the sedimentation plate method. Grown fungi were identified using standard mycological techniques, including determining macroscopic and microscopic characteristics. Results: A total of 230 colonies belonging to 11 genera of fungi were isolated from all culture media. Fungi were isolated from 53 (80.3%) samples. The highest and lowest percent of positive samples were found in outdoor (100%) and hospital (64.3%) air samples, respectively. Cladosporium (43.9%) and Aspergillus (36.4%) were the most common fungi isolated from the samples. Rhodotorula, Syncephalastrum, Paecilomyces, Mucor, and Acremonium were the least isolated fungi (1.5%). Conclusion: This study showed the difference in the diversity and distribution of fungi in different environments. Cladosporium and Aspergillus were the most common fungi isolated, which need to be considered due to their ability to cause various diseases.