Two experiments tested the motivational role of the US in classical conditioning of the rabbit’s nictitating membrane (NM) response. In Experiment 1, subjects were trained to an intermediate performance level and then given a series of (1) CS-US trials, (2) “backwards” US-CS trials, (3) CS-alone trials, (4) US-alone trials, or (5) no-stimulus presentations. Interpolated presentations of the US, either alone or in a backwards contingency, tended to produce an impairment of subsequent acquisition. In Experiment 2, subjects were trained with strong or weak US intensity on paired or interpolated trials. US intensity on interpolated trials had only a very small effect, whereas the effect of US intensity on paired trials was quite large. Shifts in paired-trial US intensity produced corresponding shifts in performance, but shifts in the intensity of the interpolated US produced no apparent effect. We conclude that the arousal of motivation is not sufficient to maintain performance in classical NM conditioning.