Crown Root Research Articles

Persistent legacy effects on soil metagenomes facilitate plant adaptive responses to drought.

Both chronic and acute drought alter the composition and physiology of soil microbiomes, with implications for globally important processes including carbon cycling and plant productivity. When water is scarce, selection favors microbes with thicker peptidoglycan cell walls, sporulation ability, and constitutive osmolyte production (Schimel, Balser, and Wallenstein 2007)-but also the ability to degrade complex plant-derived polysaccharides, suggesting that the success of plants and microbes during drought are inextricably linked. However, communities vary enormously in their drought responses and subsequent interactions with plants. Hypothesized causes of this variation in drought resilience include soil texture, soil chemistry, and historical precipitation patterns that shaped the starting communities and their constituent species (Evans, Allison, and Hawkes 2022). Currently, the physiological and molecular mechanisms of microbial drought responses and microbe-dependent plant drought responses in diverse natural soils are largely unknown (de Vries et al. 2023). Here, we identify numerous microbial taxa, genes, and functions that characterize soil microbiomes with legacies of chronic water limitation. Soil microbiota from historically dry climates buffered plants from the negative effects of subsequent acute drought, but only for a wild grass species native to the same region, and not for domesticated maize. In particular, microbiota with a legacy of chronic water limitation altered the expression of a small subset of host genes in crown roots, which mediated the effect of acute drought on transpiration and intrinsic water use efficiency. Our results reveal how long-term exposure to water stress alters soil microbial communities at the metagenomic level, and demonstrate the resulting "legacy effects" on neighboring plants in unprecedented molecular and physiological detail.

Herbicide Stress Inducesbeetle Oviposition on Red Maples.

Flatheaded borers (FHB; Chrysobothris spp.), are woodboring-beetles that lay their eggs in the bark and cambium of deciduous trees in North America. Females often target stressed host-plants for oviposition. The reason why is unknown; however, stressed plants often suffer various induced phytochemical changes that may enhance larval infestation success depending on the stressor such as induced upregulation of defenses, reallocation of nutrients, and changes to volatile organic compound (VOC) emissions. To understand attraction of FHB to specific stress-induced changes, we analyzed phytochemical changes associated with stress treatments and attractiveness maple trees to FHB. Trees were stressed by: (1) chemical stress (pelargonic acid herbicide), (2) physical stress (physically removing leaves), and (3) physical stress (removing portions of bark near the root crown). After reflush of defoliated trees, bark tissues where FHB larvae feed were analyzed for nutritional changes (carbon and nitrogen), anti-nutritive changes (polyphenols and tannins) and emissions of foliar VOCs. At the end of the growing season, trees were assessed for FHB larval presence and oviposition attempts. There were more larvae and oviposition attempts on trees stressed by herbicide application. Compared to other treatments, herbicide-stressed trees had greater nitrogen and total polyphenol concentrations. Greater nitrogen may play a role in the fitness of feeding larvae, and the greater polyphenol concentration may stimulate female oviposition in the herbicide stressed trees. Females may be able to locate the herbicide-stressed trees by using volatile cues such as increases in limonene, α-farnesene, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and hexenyl acetate.

A Mechanistic Prediction Model of Resistance to Uprooting of Coniferous Trees in Heilongjiang Province, China.

Coarse roots and the root plate play an important role in tree resistance to uprooting. In this study, a qualitative mechanistic model was developed to analyze coniferous tree resistance to uprooting in relation to tree morphological characteristics. The sizes of the crown, stem, and root plate of twenty sample spruces and twenty sample Korean pines were individually measured for this purpose. Using Ground Penetrating Radar (GPR), the coarse root distribution and root plate size were detected. In the qualitative mechanistic model, a larger crown area increased the overturning moment, while higher DBH and root plate mass increased the resistance moment. The resistance coefficient (Rm) was calculated by comparing resistive and overturning moments, classifying samples into three uprooting hazard levels. Trees with smaller crown areas, larger stems, and root plates tend to have higher resistance to uprooting, as indicated by higher Rm values. This qualitative mechanistic model provides a useful tool for assessing coniferous standing tree uprooting resistance.

Evaluation of Chemical and Biological Products for Control of Crown Gall on Rose.

Crown gall is a soil-borne bacterial disease caused by Agrobacterium tumefaciens, leading to significant economic losses in many plant species. For the assessment of the biological and chemical products on crown gall, each plant's crown region and roots were wounded, and then were dipped into their respective treatments. After the treatments, the plants were inoculated with a suspension of pathogenic A. tumefaciens isolate FBG1034 and maintained in a greenhouse for six months to assess them for gall formation. A quantitative real-time PCR assay was performed to quantify the A. tumefaciens using the chvE gene. Biological products such as the Agrobacterium radiobacter strain K1026, and strains 1 and 2, resulted in the lowest average root gall diameter and significantly reduced the crown gall diameter to stem diameter ratio, and the chemical product copper octanoate reduced the number of crown and root galls as well as the crown and root gall diameter compared to the inoculated, non-treated control. Moreover, both the A. radiobacter strain K1026 and strain 1 treatments resulted in an approximately 85% and 65% reduction in crown and root gall incidence, respectively, in both of the trials compared to the inoculated, non-treated plants. The findings of this study indicate that the use of biological and chemical products could help to suppress crown and root gall disease in rose plants.

Automated Analysis of Tooth Anatomy and Pathological Conditions from Orthopantomogram using Deep Neural Networks

This research project aims to automate the identification, labeling, and counting of teeth, as well as the classification of abnormalities and detection of caries in dental X-rays, specifically orthopantomograms (OPGs). It involves several deep neural networks and learning algorithms. The first module uses semantic segmentation with a U-net model to create masks for tooth detection, which are then refined with the YOLOv3 detector, achieving 80% accuracy. Canonical correlation analysis (CCA) helps find tooth midpoints and count the total number of teeth. The second module classifies abnormalities and pathologies using transfer learning with the Inceptionv3 model, yielding moderate accuracy. Caries detection is performed with thresholding and segmentation. The third module detects three treated pathologies—root canal treatments, crowns, and implants—using Faster RCNN and Inceptionv3, showing fair accuracy. Overall, the automated approach demonstrates promising results for enhancing X-ray image interpretation and diagnosing oral diseases.

Reattachment of Fractured Tooth Segment in Minimally Invasive Way - A Case Report

Dental trauma frequently results in coronal fractures of the front teeth. Root canal therapy followed by reattaching the fractured segment with fiber post reinforcement is a viable alternative in cases of complex fractures where the fractured segment is present and there is close approximation of the segment to the intact tooth. Reattaching a fractured piece is a quicker, less involved process that offers immediate relief, better aesthetics, and function restoration. The present case report is of a 11-year-old boy with an uncomplicated crown fracture of permanent maxillary right central incisor and a palatal uncomplicated crown–root fracture of his permanent maxillary left central incisor . The fractured fragment was reattached using nano-filled dual-cure composite resin and tooth restored with composite resin. The fragment showed opaque discoloration that reduced over time but still persisted after 1 year. During the follow-up period, no unfavorable radiographic changes were seen. The prognosis of the teeth was favorable with good esthetic results. Keywords: Fragment reattachment, Fragment rehydration, Periodontal health, Root fracture, Uncomplicated crown.

RELATIONSHIP OF THE IMPACTED MAXILLARY CANINE WITH THE MORPHOLOGY OF ADJACENT LATERAL INCISOR - A RETROSPECTIVE STUDY

Objective:This study aimed to inquireabout the location and inclination of the maxillary-impacted canine and its correlation with the morphology and root resorption of the adjacent lateral incisor by using CBCT. Study design: The samples under investigation comprised 92 diagnosed cases of maxillary impacted canine, consisting of 35 males and 57 females, who met the studys inclusion criteria. Among these cases, 87 were unilateral, while 5 presented bilateral impactions, totaling 97 impacted canines. The morphological measurement of the lateral, angles between the central axis of the lateral incisor to the midline and the palatal plane,in both the impacted and non-impacted sides, were measured and compared. Results: Statistically significant differences were obtained when comparing the morphological (crown length, root length, mesiodistal and buccolingual width of crown) and angular parameters (inclination to palatal plane, angulation to MSR) of the lateral incisor, root resorption of the lateral incisor with canine overlapping, canine cusp tip level with root resorption of the adjacent lateral incisor, canine angulation with root resorption of the adjacent lateral incisor. Conclusion:The association between the morphologic and angular features of the maxillary lateral incisors and maxillary canine impaction was confirmed. Key predictors of maxillary canine impaction were identified as the crown and root length of the lateral incisor, the mesiodistal and buccolingual widths of the lateral incisor crown, and the angulation of both the lateral incisor and canine to the midline.

Projecting Climate Change Impact on Precipitation Patterns during Different Growth Stages of Rainfed Wheat Crop in the Pothwar Plateau, Pakistan

In rainfed areas, precipitation variations directly impact wheat growth stages such as emergence, tillering, jointing and booting, and maturity. Evaluating the impact of climate change on precipitation patterns during these critical growth stages is crucial for adapting climate change and ensuring global food security. In this study, projections of five General Circulation models (GCMs) under two shared socioeconomic pathways (SSPs) were used to predict the changing characteristics of precipitation during four main growth stages of wheat in the rainfed region of the Pothwar Plateau, Pakistan. Historical datasets of daily precipitation at six weather stations were analyzed to check the past changes in the precipitation patterns. During the baseline period (1985–2014), the annual average precipitation decreased at a rate of −9.75 mm/decade, while the amount of precipitation during the rabi season (wheat-growing season) decreased at a rate of −20.47 mm/decade. An increase in the precipitation was found during the fourth (flowering) stage of crop growth, while the first three stages experienced a decrease in the precipitation amount. The multimodal ensembled data, under the SSP2-4.5 scenario, revealed a significant decline (at the rate of −16.63 mm/decade) in the future annual precipitation. However, it is projected that, under SSP2-4.5, there may be a slight increase (4.03 mm/decade) in the total precipitation amount during the future rabi season. Under the SSP5-8.5 scenario, average annual precipitation exhibited a slightly increasing trend, increasing by 1.0 mm/decade. However, during the rabi season, there was a possibility of a decrease in precipitation amount, with a rate of 11.64 mm/decade. It is also expected that the precipitation amount may vary significantly during the crown root initiation, jointing and booting, and flowering stages in the near future. These results provide a framework for the planning of wheat production in the Pothwar region of Pakistan, taking into account the potential impact of shifting weather patterns, particularly in terms of uneven precipitation.

Rhizosphere hydraulic regulation in maize: tailoring rhizosphere properties to varying soil textures and moistures

Abstract Aims Motivated by recent studies highlighting the active role of plants in influencing the hydraulic properties of the rhizosphere through mucilage exudation, this study explored the effect of plant rhizosphere regulation on the emergent hydraulic properties of the rhizosphere under varying soil texture and moisture levels. Methods Maize (Zea mays) plants were cultivated in sand and loam soils exposed to varying moisture levels. Neutron radiography was employed to quantify the profile of water content ($${\theta }_{r})$$ θ r ) around lateral and crown roots. The $${\theta }_{r}$$ θ r were used to derive rhizosphere hydraulic indices such as rhizosphere extension ($${d}_{rh}$$ d rh ), water content at the root-soil interface ($${\theta }_{rs}$$ θ rs ), and water content in the rhizosphere ($${\theta }_{rh}$$ θ rh ). The latter two attributes were normalized by the respective bulk soil water contents ($${\theta }_{rs}^{*}$$ θ rs ∗ , $${\theta }_{rh}^{*}$$ θ rh ∗ ). Results Results showed a higher $${\theta }_{rs}^{*}$$ θ rs ∗ , $${\theta }_{rh}^{*}$$ θ rh ∗ and $${d}_{rh}$$ d rh in coarse-textured soil versus fine-textured soil. This indicates a more pronounced rhizosphere development in sand compared to loam, possibly via mucilage exudation to maintain better root-soil contact. In contrast, soil water content did not impact the rhizosphere properties of crown roots but impacted the rhizosphere properties of lateral roots. The derived rhizosphere water retention curves revealed a higher water-holding capacity of the rhizosphere in both soils compared to bulk soil. Conclusions Our study underscores that soil-grown maize plants dynamically adjust the hydraulic properties of their rhizosphere in response to external factors, primarily aiming to optimize root-soil contact. That leads to a more pronounced rhizosphere in coarser textures, interacting with the soil moisture.

Foliar Application of Silicon Influences Crop Productivity, Dry Matter Accumulation, Water Use Efficiency, Lodging Score, and Aphid Density in Wheat (Triticum aestivum L.)

ABSTRACT Silicon (Si) is a versatile nutrient that plays an instrumental role in mitigating biotic and abiotic stresses besides improving growth and yield of graminaceous crops. We hypothesized that application Si would significantly improve productivity and resilience of wheat. Hence, the objectives were a) to assess the impact of Si application on wheat growth, productivity, nutrient uptake, water use efficiency, and b) to determine the potentiality of Si in mitigating lodging and aphid density. Therefore, we conducted a field experiment with five levels of Si (0, 2, 4, 6, and 8 g Si liter−1) at three growth stages (crown root initiation, tillering, and jointing stage) using a factorial randomized block design replicated three times. The results showed that increasing Si doses positively influenced plant height, dry matter accumulation (DMA), leaf area index (LAI), yield, and nutrient uptake. The highest grain and straw yield were observed with 8 g Si liter−1, followed by 6 g Si liter−1, while the control had the lowest yields. With 8 g Si liter−1, grain yield, straw yield, and Si uptake increased by 10.5%, 13.5%, and 26.3%, respectively, compared to the control. Additionally, Si application at 8 g Si liter−1 significantly reduced the density of S. avenae (aphids) by 81.4% and lodging by 75.1% compared to the control. Overall, the study demonstrated that increasing Si doses enhanced various growth and yield parameters, with 8 g Si liter−1 and 6 g Si liter−1 showing superior results. Among the growth stages, foliar application of Si during the tillering stage exhibited better performance in terms of growth, yield, and nutrient uptake in wheat. Therefore, the study concludes that Si fertilization at a rate of 8 g Si liter−1 during the tillering stage can effectively improve growth, productivity, and nutrient uptake in wheat in the southern region of Rajasthan.

OsNAC121 regulates root development, tillering, panicle morphology, and grain filling in rice plant.

Transcription factors in coordination with phytohormones form an intricate regulatory network modulating vital cellular mechanisms like development, growth and senescence in plants. In this study, we have functionally characterized the transcription factor OsNAC121 by developing gene silencing and overexpressing transgenic rice plants, followed by detailed analyses of the plant architecture. Transgenic lines exhibited remodelling in crown root development, lateral root structure and density, tiller height and number, panicle and grain morphologies, underpinning the imbalanced auxin: cytokinin ratio due to perturbed auxin transportation. Application of cytokinin, auxin and abscisic acid increased OsNAC121 gene expression nearly 17-, 6- and 91-folds, respectively. qRT-PCR results showed differential expressions of auxin and cytokinin pathway genes, implying their altered levels. A 47-fold higher expression level of OsNAC121 during milky stage in untransformed rice, compared to 14-day old shoot tissue, suggests its crucial role in grain filling; as evidenced by a large number of undeveloped grains produced by the gene silenced lines. Crippled gravitropic response by the transgenic plants indicates their impaired auxin transport. Bioinformatics revealed that OsNAC121 interacts with co-repressor (TOPLESS) proteins and forms a part of the inhibitor complex OsIAA10, an essential core component of auxin signalling pathway. Therefore, OsNAC121 emerges as an important regulator of various aspects of plant architecture through modulation of crosstalk between auxin and cytokinin, altering their concentration gradient in the meristematic zones, and consequently modifying different plant organogenesis processes.

Rapid and quantitative detection of Aspergillus niger Van Tieghem using loop-mediated isothermal amplification assay

AbstractPeanut (Arachis hypogaea L.) crown rot and root rot are common diseases caused by Aspergillus niger Van Tieghem. Early and accurate detection of A. niger is key to disease management. In this study, the design of two to five sets of loop-mediated isothermal amplification (LAMP) primers was based on the EglA, GOD, Tub, NRPS, Tan, CbhA, and CbhB genes of A. niger. Of these, primer set GOD-91 was selected for optimization of the three-factor LAMP system: the Bst DNA polymerase concentration, the concentration ratio of the inner and outer primers, and the concentration of Mg2+. In addition, the optimized LAMP reaction system for A. niger detection was validated for specificity, sensitivity, and on-site feasibility. The specificity test showed that A. niger could be specifically detected with the proposed method without cross-amplification of other pathogenic fungi DNA. Moreover, based on the sensitivity test, the lowest detection limit of this reaction system was 5.1 × 10−7 ng/µL pAN01 plasmid DNA, after which a standard curve was generated for the quantitative detection of A. niger. The LAMP method was further applied for field sample assessment before and after A. niger infection, successfully detecting A. niger presence in the samples collected in the field. This study yielded a sensitive, specific, and reproducible LAMP system that can be used to assess on-site samples within 45 min. It is an effective approach for the rapid and quantitative detection of A. niger.

Fungal Species Causing Canker and Wilt of Ficus carica and Evidence of Their Association by Bark Beetles in Italy.

Field surveys conducted during 2021 and 2022 in Western Sicily, Italy, revealed the presence of common fig trees severely affected by trunk and crown root canker and bark cracking. Moreover, in conjunction with the symptomatic tissues, the same surveyed plants showed the presence of bark beetle holes and internal wood galleries. The predominant beetle Criphalus dilutus was previously reported attacking figs in Sicily. Phylogenetic analyses based on multilocus DNA data showed the presence of different fungal taxa associated with disease symptoms, including Botryosphaeria dothidea, Ceratocystis ficicola, Diaporthe foeniculina, Neocosmospora bostrycoides, N. perseae, and Neofusicoccum luteum. Pathogenicity tests conducted on potted fig plants showed that all the species were pathogenic to fig, with C. ficicola and Neocosmospora spp. as the most aggressive fungal species. Moreover, isolations conducted from the bodies of emerging adult insects recovered from disease samples confirmed the presence of C. ficicola and Neocosmospora spp., suggesting the potential involvement of C. dilutus in their dissemination.

Sex-specific strategies of resource utilization and determining mechanisms of Hippophae rhamnoides in response to community succession

Abstract The dioecious plant, Hippophae rhamnoides, is a pioneer species in community succession on the Qinghai-Tibet Plateau (QTP), plays great roles in various ecosystem services. However, the males and females of the species differ both in their morphology and physiology, resulting in a change in the ratio of male to female plants depending on the environment. To further explore the functional traits critical to this sex-based distinctive response in the alpine grassland, we have surveyed the sex ratios, measured their photosynthetic parameters, height, leaf area and biomass allocation. The results showed that (i) The males had higher Pn, light saturation point, apparent quantum efficiency, Amax and lower water-use efficiency (WUE), which exhibited higher utilization efficiency or tolerance to strong light, while the females indicated higher utilization efficiency for low light and water. And it showed sex-specific biomass allocation patterns. (ii) H. rhamnoides populations across the successional stages all showed a male-biased sexual allocation, which was closely related to sex-specific WUE, Pn, root biomass/total biomass and root–crown ratio. (iii) The leaf traits of H. rhamnoides changed from higher Narea, Parea and leaf mass per area in the early and late to lower in the middle, which meant they moved their growth strategy from resource rapid acquisition to conservation as the succession progressed. (iv) The increasing soil total phosphorus mostly contributed to regulating the sex bias of populations and variations of traits during the succession. The results are vital for the management of grassland degradation and restoration due to shrub encroachment on the QTP.

An improved method to study Phytophthora cinnamomi Rands zoospores interactions with host

Phytophthora cinnamomi Rands is a highly prevalent phytopathogen worldwide, ranking among the top ten in terms of distribution. It inflicts crown rot, canker, and root rot on numerous plant species, significantly impacting the biodiversity of both flora and fauna within affected environments. With a host range spanning over 5,000 species, including important plants like Quercus suber, Quercus ilex, Castanea sativa, and commercially significant crops such as avocado (Persea americana), maize (Zea mays), and tomato (Solanum lycopersicum), Phytophthora cinnamomi poses a substantial threat to agriculture and ecosystems. The efficient dissemination of the oomycete relies on its short-lived asexually motile zoospores, which depend on water currents to infect host roots. However, managing these zoospores in the laboratory has long been challenging due to the complexity of the life cycle. Current protocols involve intricate procedures, including alternating cycles of growth, drought, and flooding. Unfortunately, these artificial conditions often result in a rapid decline in virulence, necessitating additional steps to maintain infectivity during cultivation. In our research, we sought to address this challenge by investigating zoospore survival under various conditions. Our goal was to develop a stable stock of zoospores that is both easily deployable and highly infective. Through direct freezing in liquid nitrogen, we have successfully preserved their virulence. This breakthrough eliminates the need for repeated culture transfers, simplifying the process of plant inoculation. Moreover, it enables more comprehensive studies of Phytophthora cinnamomi and its interactions with host plants.

ZmPILS6 is an auxin efflux carrier required for maize root morphogenesis

Plant root systems play a pivotal role in plant physiology and exhibit diverse phenotypic traits. Understanding the genetic mechanisms governing root growth and development in model plants like maize is crucial for enhancing crop resilience to drought and nutrient limitations. This study focused on identifying and characterizing ZmPILS6, an annotated auxin efflux carrier, as a key regulator of various crown root traits in maize. ZmPILS6-modified roots displayed reduced network area and suppressed lateral root formation, which are desirable traits for the "steep, cheap, and deep" ideotype. The research revealed that ZmPILS6 localizes to the endoplasmic reticulum and plays a vital role in controlling the spatial distribution of indole-3-acetic acid (IAA or "auxin") in primary roots. The study also demonstrated that ZmPILS6 can actively efflux IAA when expressed in yeast. Furthermore, the loss of ZmPILS6 resulted in significant proteome remodeling in maize roots, particularly affecting hormone signaling pathways. To identify potential interacting partners of ZmPILS6, a weighted gene coexpression analysis was performed. Altogether, this research contributes to the growing knowledge of essential genetic determinants governing maize root morphogenesis, which is crucial for guiding agricultural improvement strategies.

Water productivity, yield response factors, yield and quality of alfalfa cultivars in semi-arid climate conditions

This study was carried out for 2 years (2020 and 2021) to elucidate the effects of water stress on yield and quality of alfalfa cultivars, to determine the water-yield and quality relations and the susceptibility of cultivars to water stress. Three different irrigation treatments (100%, 75% and 50% of full irrigation, I100, I75 and I50) and 12 different alfalfa cultivars (SHN, Osmanağa, Şahin 42, Minerva, Elçi, Riviera Vicentina, Magna 601, Magnum V, Prosementi, Ezzelina, Sunter, Nimet) were used in present experiments conducted in randomized-blocks split-plots design with 3 replications to investigate effects of irrigation levels on water, yield and quality parameters of alfalfa. Present findings revealed that water stress decreased crop water productivity, but increased irrigation water productivity of alfalfa cultivars. Herbage yields of the cultivars varied between 48.20 and 64.98 t/ha, hay yields between 11.42 and 14.67 t/ha and protein yields between 2.22 and 3.07 t/ha. Alfalfa was found to be sensitive to water stress in terms of herbage yield, but more tolerant in terms of hay and protein yields. Morphological characteristics (plant height, number of stems per plant, number of main stem branches and root crown diameter) decreased with increasing water stress levels. In terms of quality traits, while acid detergent fiber (ADF), neutral detergent fiber (NDF), crude fiber and crude ash ratios increased, crude protein ratios decreased with increasing irrigation water levels. It was concluded based on present findings that water stress resulted in different responses of different alfalfa cultivars and although it increased quality traits in general, it decreased herbage, hay and protein yields. Again, based on present findings, Magna 601 cultivar could be recommended for semi-arid regions.

Transcriptional changes during crown-root development and emergence in barley (Hordeum vulgare L.)

BackgroundRoots play an important role during plant growth and development, ensuring water and nutrient uptake. Understanding the mechanisms regulating their initiation and development opens doors towards root system architecture engineering.ResultsHere, we investigated by RNA-seq analysis the changes in gene expression in the barley stem base of 1 day-after-germination (DAG) and 10DAG seedlings when crown roots are formed. We identified 2,333 genes whose expression was lower in the stem base of 10DAG seedlings compared to 1DAG seedlings. Those genes were mostly related to basal cellular activity such as cell cycle organization, protein biosynthesis, chromatin organization, cytoskeleton organization or nucleotide metabolism. In opposite, 2,932 genes showed up-regulation in the stem base of 10DAG seedlings compared to 1DAG seedlings, and their function was related to phytohormone action, solute transport, redox homeostasis, protein modification, secondary metabolism. Our results highlighted genes that are likely involved in the different steps of crown root formation from initiation to primordia differentiation and emergence, and revealed the activation of different hormonal pathways during this process.ConclusionsThis whole transcriptomic study is the first study aiming at understanding the molecular mechanisms controlling crown root development in barley. The results shed light on crown root emergence that is likely associated with a strong cell wall modification, death of the cells covering the crown root primordium, and the production of defense molecules that might prevent pathogen infection at the site of root emergence.

Colonization of Solanaceous crops by endophytic and rhizospheric plant growth-promoting bacteria from the native Solanaceae Solanum hindsianum Benth

Plant-associated bacteria are an important tool for sustainable agriculture since the have been shown to play a significant role in the health and productivity of plants through various mechanisms. However, commercial inoculants often fail to produce desired effects due to colonization issues and lack of adaptation to local conditions. In this study, we aimed to evaluate the potential of plant growth-promoting bacteria (PGPB) isolated from Solanum hindsianum, a native plant from the Sonoran Desert, to colonize and promote growth in Solanaceae crops under different irrigation conditions. First, endophytic bacteria were screened for various plant growth-promoting traits, and selected strains were identified as Bacillus, Brevibacillus, Pseudomonas, Acinetobacter, and Siccibacter. Then, endophytic and rhizospheric Pseudomonas isolates, were tested for their tolerance to salinity and osmotic stress and for their ability to induce auxin-responsive gene expression and promote plant development in Arabidopsis. All strains induced strong expression of an auxin reporter gene, while significantly improving root and plant morphology, and showed tolerance to osmotic and salinity stress. In colonization experiments, these strains successfully colonized the root crown and rhizosphere of chili, tomatillo, and tomato plants. However, the endophytic strain showed better colonization ability, especially under low irrigation conditions. Overall, this study demonstrates the potential of native PGPR strains to colonize and promote growth in Solanaceae crops under specific environmental conditions.

Identification and Evaluation of Celery Germplasm Resources for Salt Tolerance

This study evaluated the salt tolerance in 40 celery germplasm resources to clarify the different salt tolerances of celery germplasm. A gradient treatment with different concentrations of NaCl solutions (100, 200, and 300 mmol·L−1) was used to simulate salt stress. After 15 days of salt treatment, 14 indicators related to plant growth, physiology, and biochemistry were determined. The results showed that different celery varieties responded differently to salt stress. Notably, there were significant variations in below-ground dry weight, root–crown ratio, antioxidant enzyme activity, and soluble protein content among the accessions under salt stress. Principal component analysis was used to identify important indices for evaluating salt tolerance, including plant height, spread, content of soluble protein, and so on. A comprehensive evaluation was conducted utilizing the salt damage index, principal component analysis, affiliation function analysis, and cluster analysis. The 40 celery germplasms were classified into five highly salt-tolerant, seven salt-tolerant, fifteen moderately salt-tolerant, nine salt-sensitive, and four highly salt-sensitive germplasms. SHHXQ, MXKQ, XBQC, XQ, and TGCXBQ were highly salt-tolerant germplasms, and BFMSGQ, HNXQ, ZQ, and MGXQW were highly salt-sensitive germplasms. The results of this study provide a reference for the variety of celery cultivation in saline areas and lay a foundation for the selection and breeding of salt-tolerant varieties of celery.