This paper aims at experimental study of delamination process in multidirectional composite laminate exhibiting elastic couplings by using the acoustic emission (AE) technique. The mode I double cantilever beam (DCB) test was conducted on carbon/epoxy laminate with the bending-twisting elastic couplings according to the ASTM D5528 Standard. Experimental research was supported with visual high resolution registration of propagated crack tip, as well as with measurements of elastic waves generated by developing internal damages by using the acoustic emission set Vallen AMSY-5 equipped with two piezoelectric sensors. Prior to test, values of fundamental Lamb wave modes velocities were estimated and the dispersion curves were calculated. Delamination initiation point was determined by using two different AE criteria. The mode I critical strain energy release rates were calculated by using three different data reduction schemes. Different damage mechanisms during crack propagation process were quantitatively evaluated by fundamental waveform features divided into three characteristic frequency bands groups. In addition, signal energy concentration regions were assessed by using the wavelet transforms. The outcomes revealed various damage mechanisms related with delamination process, even though the matrix cracking found to be the dominating mode of failure.