Concrete is good in compression but weak in tension. The strength of concrete can be enhanced by using admixtures and biological end products during the process of mixing. The cracks appear in concrete even after taking proper measures. Bacterial induction of calcium carbonate precipitate can be a solution to cure cracking as well as increase the strength of concrete. Microbial induction using specific species of bacteria can enhance the strength of concrete as well as give it the ability to self heal the cracks by calcium carbonate precipitate.In this research paper, bacterial cells of Bacillus sp. bacteria are induced in concrete which forms a calcium carbonate precipitate by reacting with calcium lactate and oxygen along with moisture present in the cracks. Specific species of Bacillus are able to live and adapt the alkaline concrete environment till the appearance of cracks. In this research, bacterial behavior after inducing it in concrete crack and its ability to form the calcium carbonate is studied. It is found by pulse velocity testing that the reduction in velocity after 30 and 45 days is 41.82% and 44.63% respectively when spraying with bacterial culture broth. The maximum increase in compressive strength when cement is replaced with calcium lactate and water by bacterial broth is 4.6%, 11.7% and 24.5% percentage at 3, 14 days and 28 days respectively for mix design with 2.5% Calcium lactate and 20% Bacillus solution where 4 different mix design were used.. The results are highly encouraging with respect to use of strains of bacteria for self-healing purpose.