Cr Uptake Research Articles

Phytoremediation of soil co‐contaminated with uranium and chromium by sunflower (Helianthus annuus L.) enhanced with slow‐release composite chelating agent (EDTA/ammonium citrate)

AbstractBACKGROUNDSoil in uranium mining areas is contaminated by uranium and associated heavy metals, posing a significant threat to human health and ecological security. Chelating agent assisted phytoremediation is a cost‐effective and ecologically friendly remediation approach for uranium and associated heavy metals contaminated soil. In this work, a novel slow‐release composite chelating agent (SRCMC‐g‐CMCD‐EDTA/AC) was fabricated using carboxymethyl chitosan‐graft‐carboxymethyl‐β‐cyclodextrin (CMC‐g‐CMCD) as a slow‐release carrier and EDTA/ammonium citrate (AC) as a composite chelating agent through the spray drying method, which was used for phytoremediation of soil co‐contaminated with uranium and chromium.RESULTSCMC‐g‐CMCD exhibited superior slow‐release performance for both EDTA and AC in comparison with CMC and CMCD. When applied to soil contaminated with uranium (U) and chromium (Cr), SRCMC‐g‐CMCD‐EDTA/AC effectively regulated the release of U and Cr. Sunflowers (Helianthus annuus L.) grown in treated soil showed a significant increase in U and Cr uptake by 70.55% and 35.55%, respectively, and reduced leaching losses by 34.88% and 37.42%.CONCLUSIONSRCMC‐g‐CMCD‐EDTA/AC not only assists in the phytoremediation of soil co‐contaminated with U and Cr but also reduces the risk of leaching into groundwater during the soil phytoremediation process. SRCMC‐g‐CMCD‐EDTA/AC‐assisted phytoremediation technology was an effective and environmentally friendly remediation means for the removal of U and heavy metals from contaminated soils in uranium mining areas. © 2024 Society of Chemical Industry (SCI).

Enhancing the phytoextraction capacity of chromium-contaminated soil by co-addition of garbage enzymes and microelectrolytic iron-carbon fillers

Most improved strategies for phytoextraction do not achieve a synergistic enhancement of chromium (Cr) accumulation capacity and biomass. This study investigated the impacts of co-addition of garbage enzyme (GE) and microelectrolytic iron-carbon filler (MF) on soil physicochemical properties, as well as form and uptake of Cr during aging and phytoextraction process. The response of rhizosphere microbial community to co-addition and its role in enhancing the remediation performance of ryegrass was further analyzed. Co-addition of GE and MF during the 12-day aging process resulted in an increase of nutrients, a shift from an oxidising to a reducing soil environment, a decrease of Cr(VI) content, and an enhancement of soil microbial community diversity and richness, creating a suitable environment for subsequent phytoextraction. During the 40-day phytoextraction process, co-addition played a crucial role in facilitating the establishment of a complex, efficient and interdependent ecological network among soil microorganisms and contributed to the evolution of microbial community composition and functional pathways. An increase in the relative abundance of Trichococcus, Azospirillum and g_norank_f_JG30-KF-CM45 elevated soil nutrient levels, while a decrease in the relative abundance of TM7a and Brucella reduced pathogen harbouring. Meanwhile, co-addition increased the relative abundance of Bacillus, Arthrobacter and Exiguobacterium, attenuated Cr phytotoxicity and improved soil biochemical activity. These markedly diminished oxidative damage and improved ryegrass growth by reducing malondialdehyde accumulation. In addition, regular additions of GE and the increase in relative abundance of norank_fnorank_o_Microtrichales led to rhizosphere acidification, which inhibited short-term Cr immobilization and contributed to a notable increase in phytoextraction efficiency. This study presents a strategy to enhance phytoremediation efficiency and soil quality during phytoextraction of Cr-contaminated soils.

Combined application of earthworms and plant growth promoting rhizobacteria improve metal uptake, photosynthetic efficiency and modulate secondary metabolites levels under chromium metal toxicity in Brassica juncea L

Chromium (Cr) toxicity impairs essential morphological and metabolic activities in plants. The present investigation was carried out to evaluate the beneficial role of plant growth promoting rhizobacterial strains namely Pseudomonas aeruginosa (M1), Burkholderia gladioli (M2) and earthworms (Eisenia fetida) in alleviating Cr toxicity in 10 days old Brassica juncea L. The findings delineated that addition of earthworms and PGPR restored growth, boosted Cr uptake and showed upregulation of metal transporter genes (SULTR 1-4). Supplementation of rhizospheric amendments reinstated Cr induced impairment in photosynthetic attributes. Gaseous exchange attributes, the efficiency of PS II, the content of total phenols, anthocyanin and flavonoids was enhanced with application of earthworms along with PGPR. Confocal imaging of primary photosynthetic pigment (chlorophyll), accessory photosynthetic pigment (carotenoids) and total phenols showed maximum fluorescence with combined inoculation of earthworms and both microbial strains (M1M2). The gene expression analysis revealed that Phyotene synthase (PSY), Photosystem II core protein psb A, psb B were down regulated in Cr stressed seedlings which upon supplementation with earthworms and PGPR were upregulated. Further, Phenylalanine ammonialyase (PAL), chalcone synthase (CHS) were upregulated with addition of earthworms and PGPR. Increased nitric oxide content, enhanced activity and upregulation of nitrate reductase (NR) gene was observed with addition of PGPR and earthworms

Simultaneous removal of Pb(II) and Cr(VI) from a steel company wastewater using various green adsorbents: Material characterization and numerical optimization

ABSTRACT This study focuses on the simultaneous uptake of Pb(II) and Cr(VI) from industrial wastewater by walnut shell (WS), almond shell (AS), peanut shell (PS), and coconut shell (CS) adsorbents. Among the used adsorbents, the CS adsorbent exhibited the greatest BET surface area of 18.97 m2/g and porosity of 63.17% and the WS adsorbent also had the highest pore volume of 0.3536 m3/g. Lead and chromium removal were optimized using response surface methodology via a central composite design (CCD) approach. The efficiency of lead and chromium uptake from the wastewater was enhanced by increasing the concentration of WS, AS, PS, and CS adsorbents (Cads.) and decreasing the flow rate (Q) of the wastewater. Under the optimal conditions (Cads. = 0.85 g/L and Q = 2.5 mL/min), the maximum lead and chromium uptake from steel company wastewater was achieved using CS (92%) and WS (97.2%) adsorbents, respectively. The actual lead and chromium removal values were well-fitted based on a high Rpred2, confirming the validity of the CCD model. The acceptable performance of these green adsorbents in the simultaneous removal of chromium and lead from the wastewater introduces the WS, AS, PS, and CS adsorbents as inexpensive and available candidates for industrial wastewater treatment containing heavy metals.

Changes in soil properties and microbial activity unveil the distinct impact of polyethylene and biodegradable microplastics on chromium uptake by peanuts.

Microplastics (MPs) are emerging persistent pollutants, and heavy metals are typical environmental pollutants, with their coexistence potentially compounding pollution and ecological risks. However, the interactive impacts and the relevant mechanisms of heavy metal and different types of MPs in plant-soil systems are still unclear. This study investigated the differential impacts of polyethylene MPs (PE MPs) and biodegradable polybutylene adipate MPs (PBAT MPs) on chromium (Cr) uptake in peanuts, focusing on plant performance and rhizosphere soil microenvironment. Compared with nondegradable PE-MPs, biodegradable PBAT MPs produced less significant influences on plant phytotoxicity, soil Cr bioavailability, and soil properties such as pH, CEC, DOC, and MBC, with the exception of MBN in Cr-contaminated soils. Compared to the control, soil pH and cation exchange capacity (CEC) decreased by MPs, while soil-soluble carbon (DOC), microbial biomass carbon, and nitrogen (MBC and MBN) increased by MPs. Compared to the control, soil-bioavailable Cr increased by 11.8-177.8% under PE MPs treatments, while increased by 5.1-156.9% under PBAT MPs treatments. The highest Cr content in shoots and roots was observed at 500.0mg·kg-1 Cr level, which increased by 53.1% and 79.2% under 5% PE MPs treatments, respectively, as well as increased by 38.3% and 60.4% under 5% PBAT MPs treatments, respectively, compared with the control. The regression path analysis indicated that pH, MBC, MBN, and soil-bioavailable Cr played a vital role in the changes of soil properties and Cr uptake by peanuts induced by MPs. Soil bacterial community analysis revealed that Nocardioides, Proteobacteria, and Sphingomonas were reduced by the inhibition of MPs, which affected Cr uptake by peanuts. These results indicated that the peanut soil microenvironment was affected by PBAT and PE MPs, altering the Cr bioavailability and plant Cr uptake in Cr-contaminated soil.

Application of selenium to reduce heavy metal(loid)s in plants based on meta-analysis

Agricultural soils are currently at risk of pollution from toxic heavy metal(loid)s (HMs) due to human activities, resulting in the excessive accumulation of arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), mercury (Hg), manganese (Mn), lead (Pb) and zinc (Zn) in food plants. This poses significant risks to human health. Exogenous selenium (Se) has been proposed as a potential solution to reduce HMs accumulation in plants. However, there is currently a lack of comprehensive quantitative overview regarding its influence on the accumulation of HMs in plants. This study utilized meta-analysis to consolidate the existing knowledge on the impact of Se amendments on plant HMs accumulation from contaminated soil media. The present study conducted a comprehensive meta-analysis on literature published prior to December 2023, investigating the effects of different factors on HMs accumulation by meta-subgroup analysis and meta-regression model. Se application showed an inhibitory effect on plant uptake of Hg (28.9%), Cr (25.5%), Cd (25.2%), Pb (22.0%), As (18.3%) and Cu (6.00%) concentration. There was a significant difference in the levels of HMs between treatments with Se application and those without Se application in various plant organs. The percentage changes in the HMs contents of the organs varied from −13.0% to −22.0%. Compared with alkaline soil (pH > 8), Se application can reduce more HMs contents in plants in acidic soil (pH < 5.5) and neutral soil (pH = 5.5–8). For daily food plants(e.g. rice, wheat and corn), Se application can reduce HMs contents in Oryza sp., Triticum sp. and Zea sp., ranging from 14.0–20.0%. Our study emphasizes that the impact of Se on reducing HMs depends on the single or combined effects of Se concentration, plant organs, plant genera and soil pH condition.

Hexavalent chromium uptake in rice (Oryza sativa L.) mediated by sulfate and phosphate transporters OsSultr1;2 and OsPht1;1

Chromium (Cr) soil contamination is a critical global environmental concern, with hexavalent chromium (Cr[VI]) being especially perilous due to its high mobility, bioavailability, and phytotoxicity. This poses a significant threat to the cultivation of crops, particularly rice, where the mechanisms of Cr(VI) absorption remain largely unexplored. This study uncovered a competitive interaction between Cr(VI) and essential nutrients—sulfate and phosphate during the uptake process. Notably, deficiencies in sulfate and phosphate were associated with a marked increase in Cr(VI) accumulation in rice, reaching up to 76.5 % and 77.7 %, respectively. Employing q-PCR, this study identified significant up-regulation of the sulfate transporter gene, OsSultr1;2, and the phosphate transporter gene, OsPht1;1, in response to Cr(VI) stress. Genetic knockout studies have confirmed the crucial role of OsSultr1;2 in Cr(VI) uptake, with its deletion leading to a 36.1 % to 69.6 % decrease in Cr uptake by rice roots. Similarly, the knockout of OsPht1;1 resulted in an 18.1 % to 25.7 % decrease in root Cr accumulation. These findings highlight the key role of the sulfate transporter OsSultr1;2 in Cr(VI) uptake, with phosphate transporters also contributing significantly to the process. These insights are valuable for developing rice varieties with reduced Cr(VI) accumulation, ensuring the safety of rice grain production.

Insights into trehalose mediated physiological and biochemical mechanisms in Zea mays L. under chromium stress

BackgroundChromium (Cr) toxicity significantly threatens agricultural ecosystems worldwide, adversely affecting plant growth and development and reducing crop productivity. Trehalose, a non-reducing sugar has been identified as a mitigator of toxic effects induced by abiotic stressors such as drought, salinity, and heavy metals. The primary objective of this study was to investigate the influence of exogenously applied trehalose on maize plants exposed to Cr stress.ResultsTwo maize varieties, FH-1046 and FH-1453, were subjected to two different Cr concentrations (0.3 mM, and 0.5 mM). The results revealed significant variations in growth and biochemical parameters for both maize varieties under Cr-induced stress conditions as compared to the control group. Foliar application of trehalose at a concentration of 30 mM was administered to both maize varieties, leading to a noteworthy reduction in the detrimental effects of Cr stress. Notably, the Cr (0.5 mM) stress more adversely affected the shoot length more than 0.3mM of Cr stress. Cr stress (0.5 mM) significantly reduced the shoot length by 12.4% in FH-1046 and 24.5% in FH-1453 while Trehalose increased shoot length by 30.19% and 4.75% in FH-1046 and FH-1453 respectively. Cr stress significantly constrained growth and biochemical processes, whereas trehalose notably improved plant growth by reducing Cr uptake and minimizing oxidative stress caused by Cr. This reduction in oxidative stress was evidenced by decreased production of proline, SOD, POD, MDA, H2O2, catalase, and APX. Trehalose also enhanced photosynthetic activities under Cr stress, as indicated by increased values of chlorophyll a, b, and carotenoids. Furthermore, the ameliorative potential of trehalose was demonstrated by increased contents of proteins and carbohydrates and a decrease in Cr uptake.ConclusionsThe study demonstrates that trehalose application substantially improved growth and enhanced photosynthetic activities in both maize varieties. Trehalose (30 mM) significantly increased the plant biomass, reduced ROS production and enhanced resilience to Cr stress even at 0.5 mM.

Enhanced phytoaccumulation dynamics of chromium and nickel from spent engine oil-contaminated soil amended with biomass-derived bulking agent

Phytoremediation is a promising sustainable approach for the remediation of soil contaminated with inorganic and organic pollutants. In the lands affected by petroleum-derived compounds, their massive toxicity towards the plants, however, hinders the efficacy of the phytoremediation process. Hence, adopting a green approach to enhance phytoremediation is highly recommended and desirable. Here, sugarcane bagasse (SCB) was used as a bulking agent enhancing the hyperaccumulation of heavy metals chromium (Cr) and nickel (Ni) by sunflower plants from 0.5%, 2.5%, and 5% oil-contaminated soil. Accumulation of Cr and Ni was observed higher in roots than shoots. The bioconcentration factor (BCF) of Cr and Ni was higher in soil amended with SCB than that without amended. The highest rate of Ni and Cr uptake was also noticed in all treatments amended with the bulking agent except in the case of 0.5% oil-contaminated soil without SCB. Overall, the phytoremediation of oil-contaminated soil is most effective when it is amended with SCB as a bulking agent which provides water and air well to plants, improving plant growth and then increasing phytoaccumulation of heavy metals.

Efficient selective adsorption of Cr(VI) by S-doped porous carbon prepared from industrial lignin: Waste increment and wastewater treatment

Industrial lignin is a waste product of the paper industry, which contains a large amount of oxygen group structure, and can be used to treat industrial wastewater containing Cr(VI). However, lignin has very low reactivity, so how to enhance its adsorption performance is a major challenge at present. In this study, a two-stage hydrothermal and activation strategy was used to activate the lignin activity and doping S element to prepare high-performance S-doped lignin-based polyporous carbon (S-LPC). The results show that the surface of S-LPC is rich in S and O groups and has a well-developed pore structure, which is very beneficial to Cr(VI) uptake -reduction and mass transfer on the material. In the wastewater, the utmost adsorption potential of Cr(VI) by S-LPC achieved 882.83 mg/g. After 7 cycles of regeneration, the adsorption of S-LPC decreased by only approximately 18 %. Ion competition experiments showed that S-LPC has excellent specificity for Cr(VI) adsorption. In factory wastewater, the adsorption performance of S-LPC for Cr(VI) remained above 95 %, which shows the excellent performance of S-LPC in practical applications. The results are of great significance for green chemical utilization of waste lignin, treatment of industrial wastewater and sustainable development.

Fullerenes and tree-shaped/fingerprinted carbon quantum dots for chromium adsorption via microwave-assisted synthesis.

Background: Employing citric acid/dimethyl formamide (CA/DMF), two distinct types of carbon quantum dots (CQDs), tree-shaped/fingerprinted (TF-CQDs) and fullerene-like (F) were synthesized from both cellulose and carboxymethyl cellulose (CMC). Methods: Fluorescence microscopy revealed different emission colors: blue for TF-CQDs and green for F, highlighting the structural influence on light properties. Transmission electron microscopy (TEM) confirmed the intricate fingerprinted and tree-like morphology of TF-CQDs and the spherical nature of F derived from CMC. The adsorption behavior and kinetics of Cr(vi) removal from water by TF-CQDs and F were evaluated. Significant findings: Both samples demonstrated rapid Cr(vi) uptake; TF-CQDs reached equilibrium within 120 minutes compared to 240 minutes for F. Subsequent leaching led to decreased adsorption after these initial periods. Kinetic analysis revealed a first-order model for TF-CQDs, implying physical adsorption dominance. Conversely, F exhibited a better fit to pseudo-first and second-order models, suggesting combined chemical and physical mechanisms.

Isolation and characterization of chromium-resistant bacteria and their effects on germination, growth, and Cr accumulation in Capsicum annum (L.) under Cr stress

Chromium (Cr) is a well-known environmental pollutant while less information is available on the role of Cr-resistant bacteria in the alleviation of Cr-stress in chili (Capsicum annum L.) plants. Effect of Cr-resistant bacterial strains on growth and Cr uptake by chili plants was investigated. The results revealed that Cr-stress showed a negative effect on germination, photosynthesis, and relative water content but the inoculation ameliorated the plant stress. Chromium-resistant bacterial strains enhanced the shoot and root growth (33% SL, 19.7% RL), shoot and root dry weight (35%, 32.9%), relative water content (32.25%), membrane stability index (46.52%) SPAD value (50.76%), Cr concentration in shoots and roots (19.87 and 18.52 mg kg−1), bioaccumulation and translocation factor (0.396 mgkg−1), and seedling vigor index (40.8%) of plants. Chromium-resistant bacterial strains enhanced the NPK uptake while reduced Cr uptake by plants. The morphological and biochemical examination of rhizobacterial strains (and NM28) resistant to Cr-stress revealed smooth, off-white colonies of bacteria composed of rod-shaped cells which are Gram positive in reaction while negative in catalase activity. High quantities of malic acid were produced by bacterial strains under study i.e. NM8 (926.12 μgmL−2) and NM28 (992.25 μgmL−2). These strains were identified as Bacillus cereus strain NM8 and Bacillus subtilis strain NM28 through 16S rRNA sequencing. Results showed that B. cereus strain NM28 is more effective than B. cereus strain NM8 in promoting the growth of Cr-stressed Chili that might be suitable to develop biofertilizer for sustainable production of vegetables under metal stress.

Deciphering the alleviation potential of nitric oxide, for low temperature and chromium stress via maintaining photosynthetic capacity, antioxidant defence, and redox homeostasis in rice (Oryza sativa)

Sodium nitroprusside (SNP) is a potent nitric oxide (NO) donor that enhances plant tolerance to various abiotic stresses. This research aims to assess the effect of SNP application on rice seedlings subjected to individual and combined exposure to two abiotic stresses viz., low-temperature (LT) and chromium (Cr). Exposure to LT, Cr, and LT+Cr caused severe oxidative damage by stimulating greater production and accumulation of reactive oxygen species (ROS) leading to lipid peroxidation and cell membrane instability. The combined LT+CR stress more intensly increased the cellular oxidative stress and excessive Cr uptake that in turn deteriorated the chlorophyll pigments and photosynthesis, as well as effected the level of tetrapyrrole biosynthesis in rice plants. The reduction in rice seedling growth was more obvious under LT+Cr treatment than their individual effects. The exogenous application of SNP diminished the toxic impact of LT and Cr stress. This was attributed to the positive role of SNP in regulating the endogenous NO levels, free amino acids (FAAs) contents, tetrapyrrole biosynthesis and antioxidants. Consequently, SNP-induced NO decreased photorespiration, ROS generation, lipid peroxidation, and electrolyte leakage. Moreover, exogenous SNP diminished the Cr uptake and accumulation by modulating the ionic homeostasis and strengthening the heavy metals detoxification mechanism, thus improving plant height, biomass and photosynthetic indexes. Essentially, SNP boosts plant tolerance to LT and Cr stress by regulating antioxidants, detoxification mechanism, and the plant's physio-biochemical. Hence, applying SNP is an effective method for boosting rice plant resilience and productivity in the face of escalating environmental stresses and pollutants.

Cr(VI) behaves differently than Cr(III) in the uptake, translocation and detoxification in rice roots

Excessive accumulation of chromium (Cr) causes severe damage to both physiological and biochemical processes and consequently growth repression in plants. Hexavalent chromium [Cr(VI)]-elicited alterations in plants have been widely elucidated at either physiological or molecular level, whereas little is known about trivalent chromium [Cr(III)]. Here, we found that both Cr(III) and Cr(VI) significantly inhibited root growth in rice plants. However, rice plants under Cr(VI) showed significantly less inhibition in root growth than those under Cr(III) at low levels, which might be attributed to the different hormetic effects of Cr(III) and Cr(VI) on rice plants. It was unexpected that Cr(III) could be actively taken up by rice roots similarly to Cr(VI); whereas they exhibited different kinetic uptake patterns. Furthermore, root-to-shoot Cr translocation under Cr(VI) was much lower than that under Cr(III). These results indicate that the uptake, translocation, and toxicity of Cr(III) differed greatly from those of Cr(VI). Transcriptome profiling of rice roots revealed that a series of gene families involved in detoxification, including ATP-binding cassette (ABC) transporters, multidrug and toxic compound extrusion proteins (MATEs), and Tau class glutathione S-transferases (GSTUs), were significantly associated with Cr accumulation and detoxification in rice roots. In addition, much more members of these gene families were upregulated by Cr(VI) compared to Cr(III), suggesting their vital roles in Cr uptake, translocation, and detoxification, especially under Cr(VI) stress. Further comparison of gstu9 and gstu10/50 mutants with their wild type confirmed that GSTUs play complex roles in the intracellular Cr transport and redox homeostasis during Cr(III) or Cr(VI) stress. Taken together, our findings provides new insights into the differential behaviors of Cr(III) and Cr(VI) in rice roots, as well as new candidate genes such as OsABCs and OsGSTUs, to further elucidate the mechanisms of the uptake, translocation, and detoxification of Cr(III) and Cr(VI).

The Application of MgO-Modified Biochars for the Immobilization of Ni, Cu, Pb, and Cr in Stone Crushing and Mining-Polluted Soil

The objective of the present study was to investigate the impact of MgO 0.5 g/kg loaded in different organic waste materials on the properties of the modified biochars obtained. The waste materials included tea waste, wood waste, water chestnut peel, and pomegranate peel, which were used to create tea waste MgO-modified biochar (TWMgO-MBC), wood waste MgO-modified biochar (WSMgO-MBC), water chestnut peel MgO-modified biochar (WCMgO-MBC), and pomegranate peel MgO-modified biochar (PPMgO-MBC). All the MgO-modified biochars were prepared at 600 °C for 2 h and applied at 0.5 and 1% doses for the immobilization of Ni, Cu, Pb, and Cr in stone crushing and mining-polluted soil and the reduction in their uptake by pearl millet (Pennisetum glaucum) plant. The greatest fresh and dry biomasses were observed at 45.04% and 31.29%, respectively, with the application of TWMgO-MBC 1% in stone-crushing-polluted soil. The highest degree of immobilization of Ni (76.67%) was observed for the WSMgO-MBC 1% treatment, Cu (73.45%) for WCMgO-MBC 1%, Pb (76.78%) for WSMgO-MBC 1%, and Cr (70.55%) for WCMgO-MBC 1%, in comparison with the control. The maximum uptake of Ni, Cu, Pb, and Cr in the shoot of pearl millet was reduced by 78.43% with WSMgO-MBC 1%, 75.06% with WSMgO-MBC 1%, 90.81% with WCMgO-MBC 1%, and 85.71% with WSMgO-MBC 1% as compared with the control. The greatest reduction in Ni, Cu, Pb, and Cr in the root of pearl millet was observed at 77.81% with WSMgO-MBC 1%, 68.09% with WCMgO-MBC 1%, 84.03% with WCMgO-MBC 1%, and 88.73% with WCMgO-MBC 1%, in comparison with the control. The present study demonstrated that the TWMgO-MBC 1% treatment was highly effective for improving plant growth, while the WSMgO-MBC 1%, and WCMgO-MBC 1% treatments were found to be highly effective for immobilizing heavy metals in polluted soils, thus facilitating safe crop cultivation. Future studies should concentrate on the long-term application of MgO-modified biochars for the remediation of multimetal-polluted soils.

Implementing magnetically-active Sn-based nanocomposites in hexavalent chromium removal from drinking water

A novel nanocomposite consisting of Fe3O4-loaded tin oxyhydroxy-chloride is demonstrated as an efficient adsorbent for the removal of hexavalent chromium in compliance to the new drinking water regulation. This study introduces a continuous-flow production of the nanocomposite through the separate synthesis of (i) 40 nm Fe3O4 nanoparticles and (ii) multilayered spherical arrangements of a tin hydroxy-chloride identified as abhurite, before the application of a wet-blending process. The homogeneous distribution of Fe3O4 nanoparticles on the abhurite's morphology, features nanocomposite with magnetic response whereas the 10 % loaded nanocomposite preserves a Cr(VI) uptake capacity of 7.2 mg/g for residual concentrations below 25 μg/L. Kinetic and thermodynamic examination of the uptake evolution indicates a relative rapid Cr(VI) capture dominated by interparticle diffusion and a spontaneous endothermic process mediated by reduction to Cr(III). The efficiency of the optimized nanocomposite was validated in a pilot unit operating in a sequence of a stirring reactor and a rotary magnetic separator showing an alternative and competitive application path than typical fixed-bed filtration, which is supported by the absence of any acute cellular toxicity according to human kidney cell viability tests.

Combined application of zinc oxide and iron nanoparticles enhanced Red Sails lettuce growth and antioxidants enzymes activities while reducing the chromium uptake by plants grown in a Cr-contaminated soil

Soil contamination with chromium (Cr) is becoming a primary ecological and health concern, specifically in the Kasur and Sialkot regions of Pakistan. The main objective of the current study was to evaluate the impact of foliar application of zinc oxide nanoparticles (ZnO NPs) (0, 25, 50, 100 mg L−1) and Fe NPs (0, 5, 10, 20 mg L−1) in red sails lettuce plants grown in Cr-contaminated soil. Our results showed that both ZnO and Fe NPs improved plant growth, and photosynthetic attributes by minimizing oxidative stress in lettuce plants through the stimulation of antioxidant enzyme activities. At ZnO NPs (100 mgL−1), dry weights of shoots and roots and fresh weights of shoots and roots were improved by 53%, 58%, 34%, and 45%, respectively, as compared to the respective control plants. The Fe NPs treatment (20 mgL−1) increased the dry weight of shoots and the roots and fresh weights of shoots and roots by 53%, 76%, 42%, and 70%, respectively. Application of both NPs reduced the oxidative stress caused by Cr, as evident by the findings of the current study, i.e., at the ZnO NPs (100 mgL−1) and Fe NPs (20 mgL−1), the EL declined by 32% and 44%, respectively, in comparison with respective control plants. Moreover, Fe and ZnO NPs enhanced the Fe and Zn contents in red sails lettuce plants. Application of ZnO NPs at 100 mg L−1 and Fe NPs at 20 mg L−1, improved the Zn and Fe contents in plant leaves by 86%, and 68%, respectively, as compared to the control plants. This showed that the exogenous application of these NPs helped in Zn and Fe fortification in plants. At similar of concenteration ZnO NPs, CAT and APX activities were improved by 52% and 53%, respectively. Similarly, the POD contents were improved by 17% and 45% at 5 and 10 mg/L of Fe NPs. Furthermore, ZnO and Fe NPs limited the Cr uptake by plants, and the concentration of Cr in the leaves of lettuce was under the threshold limit. The exogenous application of ZnO NPs (100 mg L−1) and Fe NPs (20 mg L−1) reduced the Cr uptake in the leaves of red sails lettuce by 57% and 51%, respectively. In conclusion, ZnO and Fe NPs could be used for the improvement of plant growth and biomass as well as nutrient fortification in stressed environments. These findings not only underscore the efficacy of nanoparticle-assisted phytoremediation but also highlight its broader implications for sustainable agriculture and environmental health. However, future studies on other crops with molecular-level investigations are recommended for the validation of the results.

Aqueous removal of Cr(VI) by Citrus sinensis juice-coated multi-walled carbon nanotubes

A novel adsorbent was prepared from multi-wall carbon nanotubes (MWCNTs) and Citrus sinensis juice extract-modified MWCNTs (OJMW) and characterized using scanning electron microscopy (SEM), Fourier transform infrared spectra (FTIR), X-ray diffractometer (XRD), BET and thermogravimetric analysis. The results showed that the nanotubes exhibited good surface area, pore size, functional groups, and robust stability at elevated temperatures (308 and 313 K). The adsorbent was applied in water for the sequestration of Cr(VI). The adsorption of Cr(VI) on MWCNTs and OJMW was influenced by factors of adsorption. Optimum removal of Cr(VI) was attained at 180 min contact time, 0.05 g adsorbent dose, and solution pH 2 for MWCNTs and OJMW. The kinetic behaviour of the Cr(VI) adsorption onto MWCNTs and OJMW was governed by the pseudo-first-order and pseudo-second-order kinetic model, respectively. The equilibrium data obtained for the uptake of Cr(VI) onto MWCNTs and OJMW fitted satisfactorily with Freundlich and Langmuir isotherm models, respectively. Meanwhile, MWCNTs and OJMW exhibited monolayer adsorption capacities (qmax) of 44.72 and 12.26 g g−1, respectively, at 298 K. The adsorption process was spontaneous and feasible. The direction of energy flow in the uptake of Cr(VI) onto OJMW and MWCNTs followed endothermic and exothermic adsorption processes, respectively. The adsorption–desorption experiments showed that OJMW had good regenerative abilities.

Enhancing Cr (VI) Adsorption of Chestnut Shell Biochar through H3PO4 Activation and Nickel Doping.

A high-efficiency nickel-doped porous biochar (PCNi3) has been successfully synthesized from chestnut shell waste via a two-step chemical activation treatment with H3PO4. The influences of microstructure, surface morphology, elemental composition, surface functional groups, specific surface area, porosity, pore-size distribution, and chemical properties of the surface state on the removal of Cr (VI) from water were thoroughly investigated by using XRD, FESEM, FTIR, Raman, BET, and XPS testing methods, N2 adsorption, and XPS testing techniques respectively. The results indicate that the treatment of H3PO4 activation and nickel doping can effectively improve microstructure characteristics, thus promoting Cr (VI) adsorption capacity. The effects of initial solution pH, solution concentration, time, and temperature on remediation are revealed. The Cr (VI) uptake experiments imply that the adsorption curves of PCNi3 fit well with the Freundlich model, the pseudo-second-order kinetic model, and the Elovich model. The adsorption process of PCNi3 can be regarded as a spontaneous endothermic reaction limited by diffusion among particles and porosity. The adsorption mechanisms of PCNi3 are ion exchange, complexation, electrostatic adsorption, and coprecipitation with the assistance of surface active sites, porosity, Ni0 particles, and Ni7P3. With these advantages, PCNi3 reveals an extraordinary Cr (VI) removal capacity and a strong ability to reduce Cr (VI) to Cr (III).

Amelioration of chromium toxicity in wheat plants through exogenous application of nano silicon

Chromium (Cr) contamination in agricultural soils poses a risk to crop productivity and quality. Emerging nano-enabled strategies show great promise in remediating soils contaminated with heavy metals and enhancing crop production. The present study was aimed to investigate the efficacy of nano silicon (nSi) in promoting wheat growth and mitigating adverse effects of Cr-induced toxicity. Wheat seedlings exposed to Cr (K2Cr2O7) at a concentration of 100 mg kg−1 showed significant reductions in plant height (29.56%), fresh weight (35.60%), and dry weight (38.92%) along with enhanced Cr accumulation in roots and shoots as compared to the control plants. However, the application of nSi at a concentration of 150 mg kg−1 showcased substantial mitigation of Cr toxicity, leading to a decrease in Cr accumulation by 27.30% in roots and 35.46% in shoots of wheat seedlings. Moreover, nSi exhibited the capability to scavenge oxidative stressors, such as hydrogen peroxide (H2O2), and malondialdehyde (MDA) and electrolyte leakage, while significantly enhancing gas exchange parameters, total chlorophyll content, and antioxidant activities (enzymatic and nonenzymatic) in plants grown in Cr-contaminated soil. This study further found that the reduced Cr uptake by nSi application was due to downregulating the expression of HMs transporter genes (TaHMA2 and TaHMA3), alongwith upregulating the expression of antioxidant-responsive genes (TaSOD and TaSOD). The findings of this investigation highlight the remarkable potential of nSi in ameliorating Cr toxicity. This enhanced efficacy could be ascribed to the distinctive size and structure of nSi, which augment its ability to counteract Cr stress. Thus, the application of nSi could serve as a viable solution for production of crops in metal contaminated soils, offering an effective alternative to time-consuming and costly remediation techniques.