CP Approach Research Articles

Studies on T‐cell receptors involved in experimental autoimmune encephalomyelitis using the complementary peptide recognition approach

Based upon Blalock's complementary recognition approach, a complementary or antisense peptide (CP) was designed to the experimental autoimmune encephalomyelitis (EAE) epitope peptide, rat myelin basic protein (MBP) peptide 72-82. This peptide (EAE CP) was shown to have some sequence similarities to T-cell receptors (TCR) and MHC II molecules in a sequence homology search. Solid-phase binding assays demonstrated specific and high affinity binding (3 and 4 microM) between the EAE CP and the rat and guinea pig EAE epitope peptides (Rt72-82 and Gp69-82), respectively. This EAE CP was also found to be immunogenic in rats in an ear swelling test for delayed type hypersensitivity (DTH) reactions and an ELISA for antibody responses. However, a rabbit antibody generated to EAE CP was shown to be unable to stain the V beta 8+ EAE susceptible T-cells in immunofluorescence analyses. This EAE CP was also used in attempts to down-regulate EAE and the results showed that prior immunization with EAE CP in complete Freund's adjuvant could not prevent the Lewis rats from developing EAE. Although the data on sense-antisense peptide interaction were positive and the EAE CP was immunogenic, the inability of EAE CP to regulate EAE indicates that the CP approach may not be generally applicable.

Root Infinitives, Tense, and Truncated Structures in Dutch

In this article, I distress the syntactic properties of child Dutch root infinitives (RIs) in light of recent findings concerning the structure of adult Dutch. The data examined offer support for those analyses that treat RIs in terms of truncated structures and speak against proposals that interpret RIs as root CPs. In the discussion I focus on one analysis representative of the truncation approach, namely, that developed by Rizzi (1993; 1993/1994), and on one analysis representative of the CP approach, that developed by Boser, Lust, Santelmann, and 'Whitman (1992), which is specifically adapted to the Germanic verb-second (V2) languages. This research is based on a corpus of production data for the child, Hein, from ages 2;4 to 3;1, obtained from CHILDES (MacWhinney and Snow (1985), coders: Wijnen and Boers).