Male reproductive cycles in lizards can be continuous or seasonal depending on the environmental conditions and female availability. The aim of this study is to investigate whether adult males of the psammophilus lizard Liolaemus cuyanus present seasonal changes of reproductive traits throughout the reproductive cycle. In order to carry out this task, sampling of specimens (N = 27) was carried out monthly from October to March in Valle de Matagusanos, Ullúm, San Juan, Argentina (31°06′46.08′’ S, 68°38′03.84′’ W; altitude: 945 m), a semi-arid zone. The animals captured in different months were grouped based on reproductive criteria according to three relevant periods: pre-reproductive (PrR), reproductive (R) and post-reproductive (PsR). In order to identify and characterize these periods, several male reproductive traits were obtained, like morphometric parameters (body mass, snout-vent length, testes and abdominal fat body mass), tubular surface and estimation of the interstitial tissue coverage, combined with histological and cell seminiferous tubule observations (germ cells types, histological and ultrastructural details) and presence of sperm in the cauda epididymis. During the PrR period, which occurred during spring, well-developed testes with free tubular sperm cells were present, but these were less frequent than during the R period. On the other hand, luminal sperm cells were evident at the seminiferous tubules from the beginning of the R period, in conjunction with the maximum testes mass and seminiferous tubular surfaces. This was also coincident with a higher abundance of sperm cells in the cauda epididymal lumen. At the end of this period, the initial regression of the seminiferous epithelium was corroborated by organelle regression which was observed in the ultrastructure of spermatogenic cells. At the beginning of the PsR period, before autumn, a marked decrease in male reproductive traits above described was detected. Instead, an increase in abdominal fat body mass could be observed indicating the onset of the lethargy period. The results obtained suggest that this population of L. cuyanus has a seasonal reproductive cycle, with its reproductive activity occurring during the spring-summer months (PrR-R periods) and finishing in late summer (PsR).