Centrifuge analogue modelling illustrates the progressive development of active folds in multilayers upon a ductile substrate during layer-parallel shortening. Models simulate folding of a mechanically stratified sedimentary sequence upon migmatitic gneisses in a large hot orogen, or upon a thick basal evaporite ± shale sequence in deeper levels of fold belts. The absence of a weak low-viscosity and low-density layer at the interface promotes infolding of the cover sequence and ductile substrate, whereas a planar upper surface to the basal ductile substrate is preserved when it is present. Whilst fold style, wavelength, and deformation of the interface with the ductile substrate differ depending on whether a low-viscosity and low-density layer is present at the base of the cover sequence, there is no marked systematic curvature of fold axes as seen in previous sandbox models for fault-bend or fault propagation folding during bulk shortening. Bulk shortening of a layered sequence with relatively thick individual layers above a ductile substrate promotes a regular and upright train of buckle folds, whereas thinner layers promote a more irregular distribution of buckle folds with variable vergence, style, and amplitude. Buckle folds above a ductile substrate progressively develop during bulk shortening from open and upright, to angular and tight, and may further develop into cuspate structures above relatively weak horizons. Relatively thick weak horizons within the layered sequence during bulk shortening interrupt regular fold patterns up structural section and allow out-of-phase folds to develop above and below the weak horizon.