Course-based Undergraduate Research Experiences Research Articles

Harnessing the Topics of Cytochrome P450 Enzymology and Artemisinin to Teach a Semester-Long Biochemistry Laboratory Course

Eleven different laboratory experiments were designed and executed throughout a semester to provide a meaningful research experience for 23 undergraduate biochemistry majors at UTSA. The topic of the semester was based on the idea of exploring new aspects to enhance our understanding of how the human body uses cytochrome P450 enzymes to metabolize artemisinin, an endoperoxide-containing plant natural product used to treat malaria. Biochemical techniques introduced to the students included computational (dry lab) techniques: the use of bioinformatics tools to design plasmids for protein expression, software to analyze the 3-dimensional structure of proteins, metabolomics software to analyze enzyme-catalyzed reaction extracts by mass spectrometry, and docking software to dock ligands into the active site of proteins. In addition, students performed experimental (wet lab) techniques including: a transformation experiment to incorporate plasmid DNA into bacteria, an extraction of plasmid DNA from bacterial cells, performing enzymatic incubations and preparing samples for analysis by mass spectrometry, and running an SDS protein gel electrophoresis. The semester ended with the use of a web-based program to allow students to visualize the proteins they were studying throughout the semester with a virtual reality headset. This course was taught for the first time at the university, so this manuscript should inspire ideas for future biochemistry laboratory courses taught in the course based undergraduate research experiences (CUREs) format.

The COVID-19 and Taste Lab: A Mini Course-Based Undergraduate Research Experience on Taste Differences and COVID-19 Susceptibility.

Traditional course-based undergraduate research experiences (CUREs) are common approaches to expose students to authentic laboratory practices. Traditional CUREs typically take up most of or an entire semester, require a laboratory section or may be a standalone lab course, and require significant financial and time commitments by the institution and instructors. As such, CUREs are harder to implement at institutions with fewer resources. Here, we developed a mini-CURE, which are typically shorter in duration, called the COVID-19 and Taste Lab (CT-LAB). The CT-LAB requires significantly fewer resources ($0.05/student) and time commitment (two class periods) than traditional CUREs. CT-LAB centers around the biological relationship between COVID-19 susceptibility and taste status (non-taster, taster, and supertaster) as well as potential implications for public policy behavior. Students participated in a class-wide study where they examined if taste status was related to COVID-19 susceptibility. They found that non-tasters had a higher likelihood of testing positive previously for COVID-19 compared to tasters and supertasters. To assess student outcomes of this CURE, students completed a pre- and post-test assessment including a content test, STEM identity survey, taste test, COVID-19 history test, and a modified CURE survey. Content test scores improved while STEM identity and attitudes about science were unchanged. A direct comparison to a repository of traditional CUREs shows that the CT-LAB produced comparable benefits to traditional CUREs primarily in skills that were particularly relevant for the CT-LAB. This work suggests that mini-CUREs, even as brief as two class periods, could be a way to improve student outcomes.

Results from a CURE course project reveal novel insights into the structure of bacterial Toll/interleukin-1 receptor (TIR) domain proteins

Abstract Toll-like receptors (TLRs) are critical for innate immune signaling, and various bacteria express Toll/interleukin-1 receptor (TIR) domains to evade this signaling. Bacterial TIRs exhibit NADase activity, although it is unclear what role NADase activity plays in evasion of innate responses. To better characterize the NADase activity of the TIR from Acenitobacter baumanii (AbTIR), we used site directed mutagenesis to identify amino acids predicted to be important for NADase activity. Studies were carried out in a Course-Based Undergraduate Research Experience (CURE) involving 16–20 undergraduate research students per year. Students used Chimera to analyze the AbTIR structure and hypothesize amino acids they predicted would play a role in enzymatic activity. They then performed site-directed mutagenesis to create plasmids for expression of mutant AbTIR. Mutant TIRs were expressed, purified and tested for NADase activity. So far, our mutagenesis results suggest a potential conformational change in the NAD binding site upon NAD binding. Identifying amino acids critical for enzymatic activity may inform drug development for diseases associated with dysregulated TIR activity. In addition, the application of the CURE class to carry out these studies has provided project-based research training experiences for approximately 80 undergraduate students, about half of whom are members of groups traditionally underrepresented in science. Several students, including some who had limited research experience before completing the course, are currently pursuing graduate studies and research-based careers, highlighting the value of CURE-based projects for facilitating the inclusion of students from diverse groups in research. TVS and TO were supported by the NIH Bridges to Baccalaureate Grant R25GM058264; CP was supported by the NIH Bridges to the Doctorate Grant R25GM119970, and the CURE course was supported by an HHMI Inclusive Excellence Grant

Exploring the Leech Gut Microbiome as a Course-based Undergraduate Research Experience

Exploring the Leech Gut Microbiome as a Course-based Undergraduate Research Experience

FUTIs: an In-Person or Online Graphing, Bioinformatics, and Scientific Literacy Exercise That Explores the Presence of Antibiotic Resistance in Foodborne Urinary Tract Infections.

We developed a course-based undergraduate research experience (CURE) that gives students an opportunity to practice the process of science in a context that intersects with their everyday lives: purchasing grocery store chicken. Student mastery of concepts was assessed by pre- and postassessment questions and lab report worksheets that guided them through the process of writing a scientific paper. Learning to produce graphs from large data sets and comparing the results with published data emphasized quantitative reasoning, while working as a group and writing helped students practice scientific communication. Most students (>90%) met the learning objectives, and students in both groups reported feeling more confident producing graphs and figures; they also showed large gains in confidence and interest in bioinformatics. Lab protocols require biosafety level 2 safety guidelines; however, students in an online or dry lab setting can use the compiled data sets and whole-genome sequences to complete the objectives. Group discussions and essay prompts at the end encourage students to use evidence-based arguments to make decisions that impact the global issue of antimicrobial resistance.

A Course-Based Undergraduate Research Experience (CURE) using Ocean Plastic Microbes as a Framework that Is Impactful for Both In-Person and Online Course Modalities

A course-based undergraduate research experience (CURE) is described, both in its original, field-based and in-person design, as well as its adaptation to online delivery during the COVID-19 pandemic. The CURE experience was centered around the topic of microbes attaching to ocean plastic debris, and included connecting nontraditional undergraduate students attending a primarily teaching institution to personnel of a research-intensive institution. The CURE was implemented in nonmajors and majors’ general biology laboratory courses, as well as in an introductory microbiology lab course for health majors. Student perception of the CURE was assessed quantitatively through self-administered retrospective surveys, and qualitatively using open-ended survey questions and focus group interviews. Survey questions were grouped into four themes: (1) research at the research institution, (2) research at the teaching institution, (3) laboratory skills, and (4) interest in science. To assess impact of the CURE by modality, major and course level, theme scores were analyzed using ANOVAs (α = 0.05). Changes in student perception of learning were measured by comparing the “before” and “after” survey scores in each theme. The main source of variation in the model was time, followed by theme and major, while modality had a negligible effect. Overall, there were increases in student perception of learning across all themes across modality, major, and course level; however, not all were statistically significant. Highest gains were observed among nonmajors, while those of the majors’ upper division courses were the lowest. On the other hand, majors’ overall scores were the highest. Health majors’ survey gains were mostly between those of nonmajors and majors. Qualitative data contextualized the quantitative results. The online adaptation was a valuable alternative, especially for nonmajors, as it expanded the range of students reached, with results similar to the in-person alternative. Preliminary data show a positive effect of the CURE in student success majoring in biology.

An Explicit and Reflective Approach to Teaching Nature of Science in a Course-Based Undergraduate Research Experience

An Explicit and Reflective Approach to Teaching Nature of Science in a Course-Based Undergraduate Research Experience

What should students be able to do? Helping students recognize professional skills in our courses

Abstract While the traditional goals of undergraduate courses are often content-based, the development of career-readiness and professional skills, such as those listed by the National Association of Colleges and Employers, are increasingly recognized as important learning outcomes. As Mammalogy courses embrace more hands-on learning activities, they provide the opportunity to embed these professional skills, which are directly relevant to many careers in science. For example, many Mammalogy courses may include projects that incorporate experimental design and data analysis that focus on quantitative literacy, in addition to technical skills including small mammal trapping and handling, or preparing voucher specimens, that focus on problem-solving and attention to detail. Here, we review the professional skills that can be developed through a Mammalogy course and evaluate evidence-based approaches to build those skills into our courses. One approach, using Course-based Undergraduate Research Experiences (CUREs), provides opportunities for both student skill development and instructor research program development. Because they invite students to participate in authentic scientific inquiry—from study design and data collection, to analysis and reporting of results—students participating in CUREs reported significant gains in their comfort with several important professional skills, including conducting field procedures, formulating and analyzing data, normalizing failure, and attempting new procedures on their own. Finally, we review the literature to demonstrate how active learning approaches inherent in CUREs can help students to build familiarity with technologies and techniques for collecting and assessing data from wild mammal populations, as well as to build important professional skills such as teamwork, leadership, problem-solving, and written and oral communication.

Science communication from a course-based undergraduate research experience.

Course-based undergraduate research experiences (CUREs) provide an efficient mechanism to provide many students with an original research project. CUREs often culminate in a capstone poster presentation, but reports on these classes usually focus on the preparation and execution of the project rather than communication of the results. This article summarizes a CURE-associated research seminar that focuses on developing the communication and interpersonal skills required for the production and presentation of a conference poster. The class is designed to provide students with the tools and confidence for effective communication of their research. From the two class offerings to date, the 18 participating students have received five awards from 19 conference presentations.

Student experiences with authentic research in a remote, introductory course-based undergraduate research experience in physics

[This paper is part of the Focused Collection on Instructional labs: Improving traditions and new directions.] Participation in undergraduate research experiences (UREs) has been identified as an important way of increasing undergraduate retention, interest, and identity within the sciences. Course-based undergraduate research experiences (CUREs) have been shown to have similar outcomes to UREs but can reach a larger number of students at one time and are accessible to any student simply through enrollment in a course. One key component of a CURE is that students must participate in authentic scientific discovery in which they answer a question where the answer is initially unknown to both students and the scientific community. Here, we present student experiences with authentic research in a large, introductory physics CURE conducted remotely during the COVID-19 pandemic. We use student responses to a closed ended survey question, as well as written responses to an open-ended end-of-course assignment to investigate what aspects of real research students felt that they participated in and the extent to which students felt that they participated in authentic research. Most students in the course felt like they engaged in real-world research during the course and a large number of students highlighted their experience with authentic research when asked to describe their experience in the course more broadly. We discuss which elements of the course may have contributed to the students’ experiences of authentic research.Received 14 November 2022Accepted 3 March 2023DOI:https://doi.org/10.1103/PhysRevPhysEducRes.19.010124Published by the American Physical Society under the terms of the Creative Commons Attribution 4.0 International license. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.Published by the American Physical SocietyPhysics Subject Headings (PhySH)Research AreasEpistemology, attitudes, & beliefsInstructional materials developmentInstructional strategiesProfessional TopicsLower undergraduate studentsPhysics Education Research

A participatory study of college students' mental health during the first year of the COVID-19 pandemic.

The COVID-19 pandemic has negatively impacted college students' mental health and wellbeing. Even before the pandemic, young adults reported high mental health morbidity. During the pandemic, young adult college students faced unprecedented challenges, including campus closure and a pivot to fully online education. This study employed a novel participatory approach to a Course-based Undergraduate Research Experience (CURE) in an introductory epidemiology course to examine factors students considered important regarding their experience during the pandemic. Two groups of undergraduate students enrolled in this course (one in Fall 2020 and another in Spring 2021) and participated in the CURE. A sub-group of these students continued after the class and are authors of this article. Through repeated cross-sectional surveys of college students' peer groups in northern California in October 2020 and March 2021, this student/faculty collaborative research team evaluated depression, anxiety, suicidal ideation and several other topics related to mental health among the students' young adult community. There was a high prevalence of anxiety (38.07% in October 2020 and 40.65% in March 2021), depression (29.85% in October 2020 and 27.57% in March 2021), and suicidal ideation (15.94% in October 2020 and 16.04% in March 2021). In addition, we identified the significant burden of loneliness for college students, with 58.06% of students reporting feeling lonely at least several days in the past two weeks. Strategies that students used to cope with the pandemic included watching shows, listening to music, or playing video games (69.01%), sleeping (56.70%), taking breaks (51.65%), and connecting with friends (52.31%) or family (51.21%). Many reported distressing household experiences: more than a third reporting loss of a job or income (34.27%) in the first year of the pandemic. We explain the participatory research approach and share empirical results of these studies. We found this participatory CURE approach led to novel, experience-based research questions; increased student motivation; real-world benefits such as combatting imposter syndrome and supporting graduate school intentions; integration of teaching, research, and service; and development of stronger student-faculty relationships. We close with recommendations to support student wellbeing and promote student engagement in research.

Length of course-based undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate dehydrogenase CUREs Community (MCC).

Course-based undergraduate research experiences (CUREs) are laboratory courses that integrate broadly relevant problems, discovery, use of the scientific process, collaboration, and iteration to provide more students with research experiences than is possible in individually mentored faculty laboratories. Members of the national Malate dehydrogenase CUREs Community (MCC) investigated the differences in student impacts between traditional laboratory courses (control), a short module CURE within traditional laboratory courses (mCURE), and CUREs lasting the entire course (cCURE). The sample included approximately 1,500 students taught by 22 faculty at 19 institutions. We investigated course structures for elements of a CURE and student outcomes including student knowledge, student learning, student attitudes, interest in future research, overall experience, future GPA, and retention in STEM. We also disaggregated the data to investigate whether underrepresented minority (URM) outcomes were different from White and Asian students. We found that the less time students spent in the CURE the less the course was reported to contain experiences indicative of a CURE. The cCURE imparted the largest impacts for experimental design, career interests, and plans to conduct future research, while the remaining outcomes were similar between the three conditions. The mCURE student outcomes were similar to control courses for most outcomes measured in this study. However, for experimental design, the mCURE was not significantly different than either the control or cCURE. Comparing URM and White/Asian student outcomes indicated no difference for condition, except for interest in future research. Notably, the URM students in the mCURE condition had significantly higher interest in conducting research in the future than White/Asian students.

A CURE on the Evolution of Antibiotic Resistance in Escherichia coli Improves Student Conceptual Understanding.

We developed labs on the evolution of antibiotic resistance to assess the costs and benefits of replacing traditional laboratory exercises in an introductory biology course for majors with a course-based undergraduate research experience (CURE). To assess whether participating in the CURE imposed a cost in terms of exam performance, we implemented a quasi-experiment in which four lab sections in the same term of the same course did the CURE labs, while all other students did traditional labs. To assess whether participating in the CURE impacted other aspects of student learning, we implemented a second quasi-experiment in which all students either did traditional labs over a two-quarter sequence or did CURE labs over a two-quarter sequence. Data from the first experiment showed minimal impact on CURE students' exam scores, while data from the second experiment showed that CURE students demonstrated a better understanding of the culture of scientific research and a more expert-like understanding of evolution by natural selection. We did not find disproportionate costs or benefits for CURE students from groups that are minoritized in science, technology, engineering, and mathematics.

A Call to Assess the Impacts of Course-Based Undergraduate Research Experiences for Career and Technical Education, Allied Health, and Underrepresented Students at Community Colleges.

Course-based undergraduate research experiences (CUREs) have the potential to impact student success and reduce barriers for students to participate in undergraduate research. Literature review has revealed that, while CUREs are being implemented at both community colleges (CCs) and bachelor's degree-granting institutions, there are limited published studies on the differential impacts CUREs may have on CC students in allied health programs, career and technical education, and nursing pathways (termed "workforce" in this essay). This essay summarizes proposed outcomes of CURE instruction and explores possible reasons for limited reporting on outcomes for CC and workforce students. It also provides recommendations to guide action and effect change regarding CURE implementation and assessment at CCs. This essay is a call to action to expand the science, technology, engineering, and mathematics career development pathway to include workforce students, implement CUREs designed for workforce students, and assess the differential impacts CUREs may have on workforce student populations at CCs.

An Interdisciplinary STEM Course-Based Undergraduate Research Experience Establishes a Community of Practice and Promotes Psychosocial Gains

An Interdisciplinary STEM Course-Based Undergraduate Research Experience Establishes a Community of Practice and Promotes Psychosocial Gains

Integrating evidence-based teaching practices into the Mammalogy classroom

Abstract The teaching practices used in college science classrooms have a profound influence on which students pass their courses (and continue to major in science) and which are ‘weeded out.’ Students from traditionally marginalized backgrounds have lower grades and learning gains compared to their nonmarginalized peers in courses that rely heavily on lecture and high-stakes exams. This achievement gap narrows or disappears when instructors use student-centered, evidence-based teaching practices. These teaching practices can include actions that shape our classroom environment, communicate course material, and assess student learning. In this paper, we provide a summary of the evidence supporting the use of student-centered teaching practices, followed by examples of several effective evidence-based teaching practices that can be integrated into organismal courses. Examples include faculty mindset for inclusion, teaching practices to increase student confidence and to reduce stereotype threat, increasing course structure by spreading points among several different types of activities, several active learning methods, jigsaws, Scientist Spotlights, course-based undergraduate research experiences, and inquiry-based labs. Each example is linked to supporting resources to help instructors easily implement these practices in their classrooms. The American Society of Mammalogists endeavors to be equitable and inclusive through numerous initiatives, and modifying our teaching practices can increase equity and inclusion of future mammalogists into our own classrooms.

A course-based undergraduate research experience for bioinformatics education in undergraduate students.

With rapid development of sequencing technology and the continuous accumulation of biological big data, people who are capable of using bioinformatic skills to analyze omics data and work out biological problems are urgently needed in the workforce, which highlights the importance of developing bioinformatics skills early in the undergraduate curriculum. Meanwhile, course-based undergraduate research experience (CURE) courses have been proved to be an effective teaching format that have many advantages over traditional labs and lectures. Here we introduced an implementation of CURE course of bioinformatics data analysis and visualization for undergraduate students in major of bioinformatics and evaluated the learning outcomes. We were able to address 10 out of 15 core competencies identified by Network for Integrating Bioinformatics into Life Sciences Education. Besides, results evaluated by Laboratory Course Assessment Survey demonstrated the goals of collaboration, discovery and relevance, and iteration were accomplished in our course. Meanwhile, a significant increase in scores of final examinations and a long-term improvement on students' research ability on bioinformatics data analysis and visualization were also observed. In summary, this CURE course is useful for undergraduate students learning related knowledge and participate in authentic research in the field of bioinformatics.

Creating Meaningful Learning Opportunities through Incorporating Local Research into Chemistry Classroom Activities

Incorporating real-life context through connections to research early in the curriculum can create meaningful learning opportunities that encourage students to engage deeply with classroom content to construct chemistry knowledge. Course-based undergraduate research experiences have been successful at integrating real-life context, but are often only incorporated into upper-level courses. To provide an additional pathway to foster interaction with research, four activities from an introductory chemistry discussion class were created to incorporate authentic research connections. Care was taken to incorporate metacognitive questions designed to help students make connections between their preexisting knowledge and course content. Marzano’s taxonomy was used to analyze the cognitive complexity of tasks, which increased in the revised activities, allowing for more opportunities for knowledge construction. Audio and written work of student groups as they worked through activities was collected. Qualitative analysis of student engagement revealed that control over the content of activities to incorporate opportunities for knowledge construction is not enough to facilitate students consciously engaging in meaningful learning. If instructors wish to promote students integrating chemistry knowledge into their existing framework, course instructors, including graduate teaching assistants, need to be trained on how to properly facilitate classroom experiences to increase the likelihood of success.

Graduate teaching assistants impact student motivation and engagement in course‐based undergraduate research experiences

AbstractThe drive to broaden equitable access to undergraduate research experiences has catalyzed the development and implementation of course‐based undergraduate research experiences (CUREs). Biology education has prioritized embedding CUREs in introductory labs, which are frequently taught by graduate teaching assistants (GTAs). Thus, a CURE GTA is expected not only to teach but also to support novice student researchers. We know little about how GTAs perform as research mentors in a CURE, or how the quality of their mentorship and support impacts undergraduate students. To address this gap in knowledge, we conducted a phenomenological study of an introductory biology CURE, interviewing 25 undergraduate students taught by nine different GTAs at a single institution. We used self‐determination theory to guide our exploration of how students' autonomous motivation to engage in a CURE is impacted by perceptions of GTA support. We found that highly motivated students were more likely to experience factors hypothesized to optimize motivation in the CURE, and to perceive that their GTA was highly supportive of these elements. Students with lower motivation were less likely to report engaging in fundamental elements of research offered in a CURE. Our findings suggest that GTAs directly impact students' motivation, which can, in turn, influence whether students perceive receiving the full research experience as intended in a CURE. We contend that practitioners who coordinate CUREs led by GTAs should therefore offer curated training that emphasizes supporting students' autonomous motivation in the course and engagement in the research. Our work suggests that GTAs may differ in their capacity to provide students with the support they need to receive and benefit from certain pedagogical practices. Future work assessing innovative approaches in undergraduate biology laboratory courses should continue to investigate potenital differential outcomes for students taught by GTAs.

Using RNAi to Examine the Connection between Phenotype and Genotype in Caenorhabditis elegans to Enhance the Undergraduate Research Experience

Gene expression plays a pivotal role in the development, differentiation, and maintenance of organisms by allowing genes to encode for an observable trait (i.e., phenotype). To understand the function of a particular gene, several approaches can be taken, ranging from removing the gene entirely to targeting the product of the gene (i.e., the protein). RNA interference (RNAi) has been shown to be a powerful approach used to silence gene activity and examine the connection between DNA and protein along with controlling gene expression. The course-based undergraduate research experience (CURE) described in this article is a hands-on molecular biology lab–based lesson that allows students to examine the impact of RNAi on Caenorhabditis elegans reproduction and development through the examination of the central dogma of biology. Through these activities, students gain practice in the scientific method of inquiry by designing experiments to observe how genotype connects to phenotype and, subsequently, organism behavior.