The circular dichroism (CD) spectra of syndiotactic methacrylic polymers with histidine-containing diacyl hydrazide side chains show the presence of ordered conformations by exciton effects. Syndiotactic poly(methacrylic acid hydrazide) has been reacted with N- tert.-butyloxycarbonyl- l-histidine to yield poly(methacrylic acid-N β(N (tert.-butyloxycarbonyl) l-histidyl) hydrazide) . In a second polymer-analogous reaction, the protecting N-tert.-butyloxycarbonyl group was removed and the CD and u.v. spectra of both polymers studied in aqueous solution at various pHs. At alkaline pH, the diacylhydrazide group is deprotonated and the CD spectra show exciton doublets which arise by an ordered conformation of the polymer. At higher pH, the positive exciton effect is invariant with time, indicating a clockwise spiralization of the side chains; a time dependence arises in the range of pH = 12–13. The positive exciton effect turns within a longer period of time into a negative exciton effect, with a corresponding reversal to a counterclockwise sense of spiralization in the spatial arrangement of the side chains.
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