Cotton Genotypes Research Articles

Screening of selected cotton genotypes (Gossypium spp) for resistance to fusarium wilt disease in Tanzania.

Cotton is an important commodity in various industries across the globe; however, cotton yield is adversely affected by the existence of pathogenic fungi included Fusarium oxysporum f.sp. vasinfectum (FOV), the causative of Fusarium wilt (FW), which is among the major disease threatening cotton production in Tanzania. In this study the screening of 25 selected varieties resistance to FOV were done under greenhouse conditions by inoculating the emerged cotton seedlings with 1.0 x 108 conidia/ml of F. oxysporum f. sp. vasinfectum using root cut dip method. The fusarium wilt disease was evaluated based on foliar symptoms (disease severity scaling and vascular coloration). Out of 25 evaluated cotton candidates, three candidates F 135, HC-B4-75 and UK 66 were significantly higher resistant with lower disease severity than others, while QUIT122, IL85, PSF2-1-1 and UK91 have significantly low resistance.

Environment influence on the physiological and productive genotypes indicators in Tajikistan republic different zones

The success of breeding largely depends on the availability of a genetically diverse source material with a broad response to changing environmental conditions, its study and proper screening, as well as the identification of new sources of valuable traits and properties among ecologically remote agroecotypes that enrich the genetic potential of domestic varieties. When creating more advanced varieties that can increase the efficiency of agriculture, a special place is given to the combination of traditional breeding methods using the genetic and physiological systems of plants that contribute to the creation of high-yielding genotypes of cotton. This is due to the fact that the genotype is realized at the physiological level, and the possibilities of selection based on empirical experience and intuition are exhausted - along with huge achievements (the contribution of the variety to the formation of the crop in many countries of the world has become 30...60 %), its development has recently been marked by pronounced negative trends, characterized, in particular, by a decrease in the resistance of plants to environmental stress and a deterioration in the quality of the products obtained. The article presents the results physiological characteristics of studies as a priority direction for increasing the productivity of cotton growing. The role of the growth of number of leaves per plant as photosynthetic activity and compensatory mechanisms, elements of the crop structure in the process of breeding new varieties and lines of medium-fiber cotton during their cultivation in various ecological zones of the Republic of Tajikistan is shown.

Effect of Irrigation Regimes and Nitrogen Levels on Growth and Yield of Cotton Genotypes Under Climate of Multan

Nitrogen use efficiency (NUE) and water scarcity are the biggest problems in the agriculture sector. These problems can arise in arid and semi-arid areas with water scarcity and low soil fertility. Cotton in south Punjab faces these problems during growth and development stages, so the yield results are lower than in other cotton-producing regions. This study was designed to assess the potential of different cotton genotypes grown on various water and nitrogen (N) applications. This study involves seven cotton crop genotypes with three irrigation levels, i.e., 18, 13, and 9 irrigations with N application at 200, 250, and 300 kg ha-1 under randomized complete block design (RCBD) factorial arrangement with three replications of each treatment. The data regarding plant growth, yield, and physiology was collected. The results indicated that Genotype IUB-13 showed a higher N uptake of 0.84% among the other seven genotypes tested at (T2). Among the seven genotypes tested, NAIB-878 demonstrated a significantly higher value of lint weight 1200 g in block-1 and T2. The finding of this study concluded that higher N levels under deficit irrigation could help to reduce the yield gaps by increasing the growth of cotton genotypes under the climatic conditions of Multan.

Genotype-by-environment interaction analysis for cotton seed yield using various biometrical methods under irrigation regimes in a semi-arid region

ABSTRACT The main objective of this study was to identify high-yielding and stable cotton genotypes under normal irrigation regimes and drought stress conditions using some biometrical methods including combined analysis of variance (ANOVA), joint regression analysis (JRA), the additive main effect and multiplicative interaction (AMMI), and genotype (G) main effect plus genotype-by-environment (GE) interaction (GGE) biplot. Cotton seed yield (CSY) was found to be significantly affected by genotypes, environments, and Genotype-by-environment interaction (GEI) using combined ANOVA, JRA, and AMMI. AMMI was superior, explaining 79% of the total variability caused by GEI under drought stress conditions compared to 66% and 25% for the GGE biplot and JRA, respectively. The CSY was found to be significantly lower under drought stress conditions vs normal irrigation regimes, ranging from 9.03% (G24) to 29.91% (G15) across the five tested environments. The JRA, AMMI, and GGE biplot methods were positively correlated for classifying the genotypes for static stability. The GGE biplot was the most effective and acceptable for identifying stable genotypes and optimal environments in both irrigation regimes. All the methods compared were concordant in separating, ranking, and identifying that the G5 and G20 genotypes were highly stable across the environment as being higher-yielding and drought-tolerant.

Evaluation of Cotton Genotypes Through the Selection Index Technique

Eight upland cotton genotypes introduced from different origins (BA440, FLASH, EDESSA, SPERO, IK259, W888, MONTANA and CANDIA) in addition to the two local cultivars (Coker 310 and Lachata) were cultivated on April 22 and 23, 2022 in the Rahmaniyah region at levels (0, 1, 2, and 3 g/liter). l) of nano-chelated NPK 20-20-20 under drip irrigation conditions, and using a randomized complete block design with three replications, in order to evaluate it by creating multiple selection indices and to estimate the expected increase in seed cotton yield. The results of the analysis of variance for the studied traits (seed cotton yield, plant height, number of fruiting per plant, number of bolls per plant, average boll weight, seed index, and lint index) showed that the mean square of genotypes and levels of nano-fertilizer and their interaction was highly significant for all traits except seed index in case of nano-fertilizer levels. The selection index that included the two traits of plant height and number of bolls per plant was characterized by a high increase in efficiency compared to direct selection of the seed cotton yield, indicating the importance of selection by adopting the selection index for several traits. This selection index in the current study was considered the best due to its high efficiency, and by adopting it in evaluating the genotypes, it was found that the highest mean of the index was 7.689 for the CANDIA genotype, with a significant difference from most other genotypes, followed by the FLASH and BA440 genotypes. The sequence of local varieties adopted in the study (Coker 310 and Lachata) in terms of performance was 10 and 7, respectively. It appeared that six introduced genotypes were superior to the best local cultivars (Lachata), and all introduced genotypes were higher than the least performing local variety (Coker 310).

A Grafting Procedure for Gossypium hirsutum Based on In Vitro Grown Seedlings

Intervarietal grafting, and in some cases interspecific grafting, practiced mainly in horticultural crops has many practical applications. Grafting can also be a useful tool to perform basic physiological, biochemical, and molecular studies to better understand communication between shoot and root in a row crop, such as cotton. We have established a grafting protocol for upland cotton (Gossypium hirsutum L.) genotypes. Although less efficient, it can be used to create grafts between upland and Pima (G. barbadense) or Old-world cottons (G. arboreum or G. herbaceum). The procedure used 8-day-old seedlings grown in culture under sterile conditions to obtain the scion and rootstock. The scion consisted of the shoot apical meristem with a few leaf primordia, epicotyl, and a small portion of the hypocotyl with the cotyledons removed. The rootstock retained 2 to 3 cm of basal hypocotyl portion with the roots trimmed. Cleft grafts were assembled under aseptic conditions followed by their culture in vitro. Once established, as indicated by the healthy growth of three to four true leaves and roots, the grafts were moved to soil in small pots. After hardening the grafts on a laboratory bench for few weeks, they were transferred to larger pots and grown to maturity in a greenhouse to obtain seeds. The success rate using our protocol ranged from 90 to 100% for Coker 312 genotype and its derivative transgenic and gene edited lines. The method was also applicable to generate intervarietal and interspecific graft unions among various types of cotton plants.

Genotype × Environment Interaction for Genotype Cotton Upland Gossypium hirsutum L.

A field experiment was conducted in the summer of 2022 at the Agricultural Research Station / Daquq site, using six upland cotton genotypes (Lachata, Coker 310, pack coat, Spiro, Montana, and IK259), three levels of potassium sulfate fertilizer (0, 100, and 200) kg.h-1, and two distances between the lines (40 and 60) cm, to study growth, yield, its components, and qualitative traits. We conclude that the genotype Spiro was stable in terms of the number of fruiting branches, while the genotype Coker 310 was more stable in terms of plant height and span length at 2.5%, and the genotypes IK259 were more stable in terms of the number of bolls per plant, the index lint, and the index of uniformity, and the two genotypes Lachata and Coker 310 in terms of boll weight. Cocker 310 and Spiro genotypes were more stable in seed cotton yield per plant and uniformity index, Spiro and Montana genotypes in ginning outturn, and Lachata and Spiro genotypes in seed index, while Lachata genotype was more stable in staple length at 50% and staple strength.

Development of High-Yielding Upland Cotton Genotypes with Reduced Regrowth after Defoliation Using a Combination of Molecular and Conventional Approaches.

Cotton is an economically important crop. However, the yield gain in cotton has stagnated over the years, probably due to its narrow genetic base. The introgression of beneficial variations through conventional and molecular approaches has helped broaden its genetic base to some extent. The growth habit of cotton is one of the crucial factors that determine crop maturation time, yield, and management. This study used 44 diverse upland cotton genotypes to develop high-yielding cotton germplasm with reduced regrowth after defoliation and early maturity by altering its growth habit from perennial to somewhat annual. We selected eight top-scoring genotypes based on the gene expression analysis of five floral induction and meristem identity genes (FT, SOC1, LFY, FUL, and AP1) and used them to make a total of 587 genetic crosses in 30 different combinations of these genotypes. High-performance progeny lines were selected based on the phenotypic data on plant height, flower and boll numbers per plant, boll opening date, floral clustering, and regrowth after defoliation as surrogates of annual growth habit, collected over four years (2019 to 2022). Of the selected lines, 8×5-B3, 8×5-B4, 9×5-C1, 8×9-E2, 8×9-E3, and 39×5-H1 showed early maturity, and 20×37-K1, 20×37-K2, and 20×37-D1 showed clustered flowering, reduced regrowth, high quality of fiber, and high lint yield. In 2022, 15 advanced lines (F8/F7) from seven cross combinations were selected and sent for an increase to a Costa Rica winter nursery to be used in advanced testing and for release as germplasm lines. In addition to these breeding lines, we developed molecular resources to breed for reduced regrowth after defoliation and improved yield by converting eight expression-trait-associated SNP markers we identified earlier into a user-friendly allele-specific PCR-based assay and tested them on eight parental genotypes and an F2 population.

Biochemical Basis of Phytoimmunity Cotton Genotypes in against Leafhoppers

Biochemical Basis of Phytoimmunity Cotton Genotypes in against Leafhoppers

Source of antibiosis to Spodoptera frugiperda (Lepidoptera: Noctuidae) in mocó cotton genotypes (Gossypium hirsutum raça marie galante L. Hutch)

Spodoptera frugiperda (Lepidoptera: Noctuidae) cause damage in cotton plants since the plant emergence until maturation. The objective of this work was to evaluate antibiosis in mocó cotton genotypes (Gossypium hirsutum var marie galante L. Hutch) to S. frugiperda. Newly hatched larvae were individualized in plastic containers (100 mL) sealed with polyethylene film to prevent the insect scape, where they were fed with cotton leaves. The treatments were 27 cotton genotypes, with 40 replications for antibiosis and 20 for nutritional parameters, using a completely randomized design. The biological parameters evaluated were: larval period and viability, weight of caterpillars at ten days, pre-pupal period and viability and pupal weight at 24 hours, life cycle and total viability, adult longevity and sex ratio. Additionally, cotton genotypes were characterized by four microsatellite markers linked blue disease resistance, angular leaf spot, root-knot nematode and reniform nematode. There was high initial mortality of S. frugiperda larvae in genotypes BA0512, MA0423, PI0438, PI0432, CE0461, CE0507, RN0512, MA0425, MA0438, PI0429, MA0430, PI0440, PI0467, AC0602, PI0416, BA0477, MA0409 and MA0409 CE0467. The cotton genotypes presented antibiosis and SSR resistance markers for nematode and blue disease, highlighting that the germplasm of this species is an important source of multiple resistance for insect resistant genotype selection programs

Evaluation of Cotton Genotypes Through the Selection Index Technique

Eight upland cotton genotypes introduced from different origins (BA440, FLASH, EDESSA, SPERO, IK259, W888, MONTANA and CANDIA) in addition to the two local cultivars (Coker 310 and Lachata) were cultivated on April 22 and 23, 2022 in the Rahmaniyah region at levels (0, 1, 2, and 3 g/liter). l) of nano-chelated NPK 20-20-20 under drip irrigation conditions, and using a randomized complete block design with three replications, in order to evaluate it by creating multiple selection indices and to estimate the expected increase in seed cotton yield. The results of the analysis of variance for the studied traits (seed cotton yield, plant height, number of fruiting per plant, number of bolls per plant, average boll weight, seed index, and lint index) showed that the mean square of genotypes and levels of nano-fertilizer and their interaction was highly significant for all traits except seed index in case of nano-fertilizer levels. The selection index that included the two traits of plant height and number of bolls per plant was characterized by a high increase in efficiency compared to direct selection of the seed cotton yield, indicating the importance of selection by adopting the selection index for several traits. This selection index in the current study was considered the best due to its high efficiency, and by adopting it in evaluating the genotypes, it was found that the highest mean of the index was 7.689 for the CANDIA genotype, with a significant difference from most other genotypes, followed by the FLASH and BA440 genotypes. The sequence of local varieties adopted in the study (Coker 310 and Lachata) in terms of performance was 10 and 7, respectively. It appeared that six introduced genotypes were superior to the best local cultivars (Lachata), and all introduced genotypes were higher than the least performing local variety (Coker 310).

Exploiting Morphophysiological Traits for Yield Improvement in Upland Cotton under Salt Stress

ABSTRACT The increased land salinization threatens land productivity, food security, and economic losses. The study used a comprehensive set of morpho-physiological, biochemical, and fiber quality parameters to examine the genetic variability of 24 cotton genotypes against 15 dSm-1 salt stress. The general linear model (GLM) effect revealed significant effects of salinity for studied accessions except for lint percentage and fiber strength. The genotype × treatment effects were also significant for all studied traits, while non-significant effects were observed for seed number per boll (SNB), potassium to sodium ratio (K+/Na+), K+, peroxidase (POD), and catalase (CAT). A notable reduction for all traits was observed except for fiber fineness, superoxide (SOD), CAT, POD, carotenoid contents, and hydrogen peroxide (H2O2), which were increased under saline conditions. Based on multivariate analyses, hybrids viz. MS-71× KAHKASHAN, followed by MS-71× CRS-2007 and NS-131× CRS-2007, performed well under both normal stressed conditions. Moreover, biochemical and agronomical traits in PCA validate that the MS-71× KAHKASHAN is the most desirable genotype under both conditions. Better hybrid performance under normal and 15 dSm-1 salt stress conditions supports the hybrid adaptability under salinity stress environments. The outcome would assist breeders in developing salt-tolerant cotton varieties under climate change scenarios.

Morpho physiological analysis of cotton genotypes (G. hirsutum L) under rainfed condition

Morpho physiological analysis of cotton genotypes (G. hirsutum L) under rainfed condition

Transcriptomic analysis reveals the beneficial effects of salt priming on enhancing defense responses in upland cotton under successive salt stress.

Priming-mediated stress tolerance in plants stimulates defense mechanisms and enables plants to cope with future stresses. Seed priming has been proven effective for tolerance against abiotic stresses; however, underlying genetic mechanisms are still unknown. We aimed to assess upland cotton genotypes and their transcriptional behaviors under salt priming and successive induced salt stress. We pre-selected 16 genotypes based on previous studies and performed morpho-physiological characterization, from which we selected three genotypes, representing different tolerance levels, for transcriptomic analysis. We subjected these genotypes to four different treatments: salt priming (P0), salt priming with salinity dose at 3-true-leaf stage (PD), salinity dose at 3-true-leaf stage without salt priming (0D), and control (CK). Although the three genotypes displayed distinct expression patterns, we identified common differentially expressed genes (DEGs) under PD enriched in pathways related to transferase activity, terpene synthase activity, lipid biosynthesis, and regulation of acquired resistance, indicating the beneficial role of salt priming in enhancing salt stress resistance. Moreover, the number of unique DEGs associated with G. hirsutum purpurascens was significantly higher compared to other genotypes. Coexpression network analysis identified 16 hub genes involved in cell wall biogenesis, glucan metabolic processes, and ribosomal RNA binding. Functional characterization of XTH6 (XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE) using virus-induced gene silencing revealed that suppressing its expression improves plant growth under salt stress. Overall, findings provide insights into the regulation of candidate genes in response to salt stress and the beneficial effects of salt priming on enhancing defense responses in upland cotton.

Drought and heat stress on cotton genotypes suggested agro-physiological and biochemical features for climate resilience.

This study aimed to investigate the impact of individual drought, heat, and combined drought and heat stress on twelve cotton genotypes, including eight tolerant and four susceptible genotypes. A field experiment was carried out by employing a randomized complete block split-plot design, with treatments (control, drought, heat, drought + heat), and cotton genotypes assigned to the main plots and sub-plots respectively. The results showed that the combined stress had a more severe impact on the yield and fiber quality of cotton genotypes compared to individual stresses. Among the studied genotypes, FB-Shaheen, FH-207, MNH-886, and White Gold exhibited superior performance in regard to agronomic and fiber quality characters under combined stress environments. Physiological parameters, including transpiration rate, stomatal conductance, relative water contents, and photosynthetic rate, were significantly reduced under combined stress. However, specific genotypes, MNH-886, FH-207, White Gold, and FB-Shaheen, demonstrated better maintenance of these parameters, indicating their enhanced tolerance to the combined stress. Furthermore, the accumulation of reactive oxygen species was more pronounced under combined stress compared to individual stressors. Tolerant genotypes showed lower levels of H2O2 and MDA accumulation, while susceptible genotypes exhibited higher levels of oxidative damage. Antioxidant enzyme activities, such as superoxide dismutase, peroxidase, and catalase, increased under combined stress, with tolerant genotypes displaying higher enzyme activities. Conversely, susceptible genotypes (AA-703, KZ 191, IR-6, and S-15) demonstrated lower increases in enzymatic activities under combined stress conditions. Biochemical traits, including proline, total phenolic content, flavonoids, and ascorbic acid, exhibited higher levels in resistant genotypes under combined stress, while sensitive genotypes displayed decreased levels of these traits. Additionally, chlorophyll a & b, and carotenoid levels were notably decreased under combined stress, with tolerant genotypes experiencing a lesser decrease compared to susceptible genotypes.

Exploitation of various physio-morphological and biochemical traits for the identification of drought tolerant genotypes in cotton

BackgroundDrought is one of the limiting factors for quality and quantity of cotton lint in tropical and sub-tropical regions. Therefore, development of drought tolerant cotton genotypes have become indispensable. The identification of drought tolerant genotypes is pre-requisite to develop high yielding cultivars suitable for drought affected areas.MethodsForty upland cotton accessions were selected on the basis of their adaptability and yield. The collected germplasm accessions were evaluated at seedling stage on the basis of morphological, physiological and biochemical parameters. The experiment was conducted under controlled conditions in greenhouse where these genotypes were sown under different levels of drought stress by following factorial under completely randomized design. The data were collected at seedling stages for root and shoot lengths, relative leaf water content, excised leaf water losses, peroxidase content and hydrogen peroxide concentrations in leaf tissues.ResultsThe biometrical analysis revealed that germplasm is significantly varied for recorded parameters, likewise interaction of genotypes and water stress was also significantly varied. The cotton germplasm was categorized in eight clusters based on response to water stress. The genotype Cyto-124 exhibited lowest H2O2 content under drought conditions, minimum excised leaf water loss under stress environment was exhibited by genotypes Ali Akber-802 and CEMB-33. Overall, on the basis of morphological and biochemical traits, SL-516 and Cyto-305 were found to be drought tolerant. Genotypes 1852 − 511, Stoneville 15–17 and Delta Pine-55 showed low values for root length, peroxidase activity and higher value for H2O2 contents. On the basis of these finding, these genotypes were declared as drought susceptible. ConclusionThe categorization of cotton germplasm indicating the differential response of various parameters under the control and drought stress conditions. The recorded parameters particularly relative leaf water contents and biochemical assays could be utilized to screen large number of germplasm of cotton for water deficit conditions. Besides, the drought tolerant genotypes identified in this research can be utilized in cotton breeding programs for the development of improved cultivars.

Assessing Drought Tolerance in a Large Number of Upland Cotton Plants (Gossypium hirsutum L.) under Different Irrigation Regimes at the Seedling Stage.

The cotton plant is important since it provides raw materials for various industry branches. Even though cotton is generally drought-tolerant, it is affected negatively by long-term drought stress. The trial was conducted according to the applied experimental design as a completely randomized design (CRD) with three replications to determine a panel of 93 cotton genotypes' genotypic responses against drought under controlled conditions in 2022. All genotypes were watered with 80 mL-1 of water (100% irrigation, field capacity) until three true leaves appeared, and then water stress was applied at a limited irrigation of 75% (60 mL-1), 50% (40 mL-1), and 25% (20 mL-1) of the field capacity. After the trial terminated at 52 days, the cv. G56, G44, G5, and G86 in RL; G1, G56, G44, G86, G51, and G88 in RFW; advanced line G5, followed by the cv. G56, advanced line G44, G75, and the cv. G90 in RDW; G44, followed by G86, the cv. G56, and elite lines G13 and G5 in NLRs were observed as drought-tolerant genotypes, respectively, while G35, G15, G26, G67, and G56 in SL; G15, G52, G60, G31, and G68 in SFW; G35, G52, G57, G41, and G60 in SDW show the highest drought tolerance means, respectively. In conclusion, the commercial varieties with high means in roots, namely G86, G56, G88, and G90, and the genotypes G67, G20, G60, and G57 showing tolerance in shoots, are suggested to be potential parent plants for developing cotton varieties resistant to drought. Using the cultivars found tolerant in the current study as parents in a drought-tolerant variety development marker-assisted selection (MAS) plant breeding program will increase the chance of success in reaching the target after genetic diversity analyses are performed. On the other hand, it is highly recommended to continue the plant breeding program with the G44, G30, G19, G1, G5, G75, G35, G15, G52, G29, and G76 genotypes, which show high tolerance in both root and shoot systems.

STUDIES ON GENETIC VARIABILTY FOR YIELD AND FIBRE QUALITY TRAITS IN UPLAND COTTON (Gossypium hirsutum L.)

Thirty genotypes of cotton were studied to estimate genetic variability, heritability (broad sense) and genetic advance for 15 traits during kharif, 2022. Analysis of variance showed significant amount of variation among the genotypes for traits studied. PCV was greater than GCV for all the traits except days to 50% flowering. High PCV and GCV were recorded for number of monopodial branches, lint index, lint yield and ginning percentage. High heritability coupled with genetic advance as percent of mean was observed for number of monopodial branches, plant height, seed cotton yield, lint index, lint yield, ginning percentage and micronaire indicating preponderance of additive gene action and hence simple selection would be effective for the improvement of these traits.

Effects of multi-resistant ScALDH21 transgenic cotton on soil microbial communities

Transgenic crops are increasingly prevalent worldwide, and evaluating their impact on soil microbial communities is a critical aspect of upholding environmental safety. Our previous research demonstrated that overexpression of ScALDH21 from desiccant-tolerant moss, Syntrichia caninervis, in cotton revealed multi-resistance to drought, salt, and biotic stresses. We conducted metabarcoding using high-throughput sequencing to evaluate the effect of ScALDH21 transgenic cotton on soil microbial communities. We further conducted soil tests to analyze the chemical properties of transgenic and non-transgenic cotton, including the total content and availability of chemical elements (K, P, and N), organic matter, and pH value. Both transgenic and non-transgenic cotton fields exhibited soil pH values higher than 8. The presence of transgenic cotton significantly enhanced the availability of available K and the total content of total P in the soil. Alpha and beta diversity indices of soil microbiota showed no difference between two transgenic and non-transgenic cotton groups. Dominant clades of fungal and bacterial genera were equivalent at the phylum and genus levels in all three groups. The correlation analysis of microbial communities and soil environmental factors revealed the absence of significant differences between transgenic and non-transgenic cotton genotypes. Functional predictions of soil microbial communities indicated that microbial community function did not show significant differences between transgenic and non-transgenic cotton samples. These findings are essential for evaluating the environmental effects of transgenic crops and supporting the secure implementation of transgenic cotton.

Genetic Variability among Egyptian Extra-long Staple Cotton Genotypes for Some Economic Traits

Genetic Variability among Egyptian Extra-long Staple Cotton Genotypes for Some Economic Traits