Costa Rica Research Articles

Tourist crossing: examining the intersection of wildlife tourism attractions & animal welfare in Costa Rica

ABSTRACT Wildlife tourism attractions (WTAs) are key actors in promoting ecotourism and animal welfare. This field note focuses on the Toucan Rescue Ranch (TRR), a wildlife rescue and rehabilitation nonprofit organization based in central Costa Rica, and its efforts to promote animal welfare through education and outreach. TRR's key ecotourism challenges and achievements – particularly around wildlife protections and coalition building are explored. The article concludes with reflections on the future opportunities facing ecotourism regarding animal welfare regionally, as well as the tourism industry more broadly. These findings were gleaned from discussions with TRR staff during a physical visit as well as during Zoom calls in 2023.

Seven New Species of Besleria (Gesneriaceae) from the Mesoamerican Flora

Seven species of Besleria Plum. ex L. (Gesneriaceae) are described from the Mesoamerican flora to add to the 27 currently recognized from the region: three from types from Panama (Besleria amicitia L. E. Skog & Barrie, B. darienensis L. E. Skog & Barrie, and B. fruticosa L. E. Skog & Barrie), two from Costa Rica (B. gracilis L. E. Skog & Barrie and B. talamancana L. E. Skog & Barrie), and one each from Honduras (B. hondurensis L. E. Skog & Barrie) and Belize (B. nicolsonii L. E. Skog & Barrie), the latter also found in Guatemala and Honduras.

Drought response strategies of vascular epiphytes in isolated pasture trees in a Costa Rican tropical montane landscape.

Vascular epiphytes of tropical montane cloud forests are vulnerable to climate change, particularly as cloud bases elevate and reduce atmospheric inputs to the system. However, studies have generally focused on epiphytes in contiguous forests, with little research being done on epiphytes on isolated pasture trees. We investigated water relations of pasture-tree epiphytes at three sites located below and above the elevation of the average cloud base in Monteverde, Costa Rica. We measured sap velocity and four microclimate variables in both the dry and wet season of 2018. We also measured functional traits, including pressure volume (PV) curves, predawn/midday water potential, and various lab-based water relations traits. We used linear mixed models to assess the correlation between microclimate and sap velocity in both seasons and ANOVA to assess the variation in PV curve and water potential variables. The turgor loss point generally increased from the wettest to driest site. However, this trend was driven primarily by the increasing prevalence of leaf succulence at drier sites. Microclimatic variables correlated strongly with sap velocity in the wet season, but low soil moisture availability caused this correlation to break down during the dry season. Our results emphasize the vulnerability of cloud forest epiphytes to rising cloud bases. This vulnerability may be more severe in pasture trees that lack the potential buffer of surrounding forest, but additional research that directly compares the canopy microclimate conditions between forest and pasture trees is needed to confirm this possibility.

Key controls on water transit times along a tropical precipitation gradient

The tropics are one of the most diverse and dynamic regions on Earth. Despite their importance, our understanding of tropical hydrological processes remains a significant challenge, mostly due to limited monitoring. Here, we used high-resolution daily input–output isotope data sets from seven tropical catchments ranging in size from 1.6 to 990 km2, situated within a precipitation gradient ranging from on average 958 to 5117 mm year−1, in Australia, Costa Rica, and Ecuador. For these catchments, we estimated and compared streamflow mean transit times (MTT) with potential explanatory hydro-geomorphological and climate variables. Using a simple lumped convolution integral model with a Gamma distribution as transfer function (best-fit Kling Gupta efficiencies up to 0.92), our tropical dataset resulted in short MTTs from 49 to 497 days (0.13 – 1.4 years), together with considerable presence of new water in the stream and low damping ratios in most of the catchments. The gamma distribution alpha parameter was below the previously identified quasi-global pattern of around 0.5 in 5 out of 7 catchments. Principal Component Analysis (PCA) and a relative importance test identified rock type (%), days with zero precipitation and the water storage capacity as the most important controls on TT. In addition, the TT distribution as indicated by the Gamma distribution alpha parameter was best explained by annual evapotranspiration, altitude, and Andosol soil cover (%). Our findings identified the key controls on TT and TT distribution in fast-responding tropical catchments compared to other geomorphic and climate zones, emphasizing the value of TT as a coherent descriptor that can be used for catchment characterization.

Regeneration of the caudal fin of the evolutionary ancient tropical gar Atractosteus tropicus

BackgroundThe tropical gar (Atractosteus tropicus), a member of the Lepisosteidae family, is native to regions extending from southeastern Mexico to southern Costa Rica. This species serves as a unique bridge between tetrapods and teleosts due to its phylogenetic position, slow evolutionary rate, dense genetic map, gene similarities with humans, and ease of laboratory cultivation. As a taxonomic sister group to teleosts like the zebrafish (Danio rerio), known for its high regenerative capacity, it remains unclear whether the tropical gar shares a similar ability for regeneration.ResultsThis study aims to elucidate the caudal fin regeneration process in tropical gar through skeletal and histological staining methods. Juvenile specimens were observed over a two-month period, during which they were fed brine shrimp, and anesthetized with 1% eugenol for caudal fin amputation. Samples were collected at various days post-amputation (dpa). Alcian blue and alizarin red staining were used to highlight skeletal regeneration, particularly the formation of new cartilage, while histological staining with hematoxylin and eosin was performed to observe tissue regeneration at the amputation site.ConclusionsThe findings reveal a remarkable ability for caudal fin regeneration in juvenile tropical gar. Given the Garfish evolutionary relationship with teleosts, this opens new avenues for research into tissue regeneration across different groups of Actinopterygii.

La formación inicial en Educación Cívica. Un estudio de casos múltiples en Costa Rica

The article is a multiple case study in three Initial Training in the Teaching of Social Studies and Civic Education of two Costa Rican public universities, where the purposes of the courses in the Civic Education component are described. In order to understand the initial formation and citizenship, we start from critical theories applied to the didactics of social sciences, based on formation models that include school culture and conflict as part of the factors that allow problematizing current citizenships. The methodology is qualitative with an interpretative-descriptive approach, and the analysis of curricular documentary sources (9 course programs) was proposed to study the type of citizenship promoted in initial training. The data analysis was carried out through a comparative matrix, which was triangulated from the data. The results show a mixture of positions on citizenship and its teaching, with an inclination towards liberal and republican discourses. In addition, teaching and evaluation methodologies are traditional. The main conclusion is that citizenship is a dynamic concept that is situated from historiographical visions, and converges between liberal, republican and critical approaches.

New species and records of limpets (Mollusca, Gastropoda) from the Pacific Costa Rica Margin.

The ocean remains a reservoir of unknown biodiversity, particularly in the deep sea. Chemosynthesis-based ecosystems, such as hydrothermal vents and hydrocarbon seeps, host unique and diverse life forms that continue to be discovered and described. The present study focuses on patelliform gastropods (limpets) collected from Pacific Costa Rica Margin hydrocarbon seeps during three research cruises from 2017 to 2019. Genetic and morphological analyses revealed the presence of several new lineages within the genera Bathyacmaea Okutani, Tsuchida & Fujikura,1992, Cocculina Dall, 1882, Paralepetopsis McLean, 1990, and the family Lepetodrilidae McLean, 1988: Bathyacmaealevinae sp. nov., Paralepetopsisvariabilis sp. nov., Pseudolepetodriluscostaricensis gen. et sp. nov., and Cocculinamethana sp. nov. These investigations also expanded the known ranges of the species Pyropeltacorymba McLean, 1992 and Lepetodrilusguaymasensis McLean, 1988 to the Costa Rica Margin. This research highlights the uniqueness of gastropod fauna at the Costa Rica Margin and contributes to our understanding of the biodiversity at chemosynthesis-based deep-sea ecosystems in the face of global biodiversity loss and increased commercial interest in deep-sea resources.

Marco legal y programas de protección en América Latina y el Caribe: desafíos y oportunidades en la protección de los derechos de las personas adultas mayores

Introduction. This article provides a review of laws, regulations, and protection programs aimed at individuals over 60 years old in seven Latin American countries: Argentina, Bolivia, Chile, Colombia, Costa Rica, Mexico, and the Dominican Republic. Additionally, it examines the national and international regulations and agreements in which each of these countries participates. The main laws and protection programs for older adults in each mentioned countryare highlighted, with a focus on the aspects they address, particularly in terms of labor rights, access to healthcare, and housing. Method. documentary review of current national and international laws and conventions was conducted in each participating country. This analysis was carried out with a qualitative approach, aiming to identify areas for improvement and potential legal gaps in the protectionof the rights of older persons in these countries. Additionally, an examination was made on how these regulations address aspects related to economic security, healthcare, social participation, combating abuse and discrimination, labor rights, and access to housing. Results. The countries guarantee fundamental rights for older adults, including health, social security, housing, food, and social and culturalparticipation. However, disparities are observed in the presence of regulations and programs in specific areas. Conclusions. While the studied countries are signatories to the most important international conventions on the rights of older persons, and despite their progress in developing laws and regulations for their compliance, thisdoes not guarantee effective implementation. Therefore, further studies are needed to delve into the implementation aspect to identify possible gaps and challenges.

Trends in Antimicrobial Consumption in Tertiary Care Hospitals in Costa Rica from 2017 to 2021: A Comparative Analysis of Defined Daily Doses per 100 Bed Days and per 100 Discharges.

Background: Antimicrobial consumption (AMC) data in Latin America are scarce and usually spread out across different sources used to make AMC calculations, making it difficult to both standardize and compare regions through similar time frames. The main objective was to analyze AMC trends in Social Security tertiary care hospitals in Costa Rica in the period spanning January 2017 to December 2021, using both the defined daily dose (DDD)/100 bed days and DDD/100 discharges. Methods: This is a retrospective observational study of antimicrobial consumption. Global consumption trends were calculated and expressed as DDD/100 bed days and DDD/100 discharges. Trends in antimicrobial consumption were analyzed using a simple linear regression model to determine potential differences in antimicrobial usage throughout the study's duration. Results: A statistically significant increase in the consumption expressed in DDD/100 discharges was observed in the following groups: carbapenems, 7.6% (trend: 64.68, p < 0.0001), trimethoprim-sulfamethoxazole: 12.6% (trend: 16.45, p < 0.0001), quinolones 9.4% (trend: 36.80, p = 0.02), vancomycin 2.0% (trend: 16.30, p = 0.03), echinocandins: 6.0% (trend: 15.17, p = 0.01) and azole antifungals: 12.10% (trend: 102.05, p < 0.0001). Additionally, a statistically significant increase of 10.30% in the consumption of azole antifungals expressed in DDD/100 bed days was observed (p = 0.0008). In contrast, a statistically significant decrease in consumption, expressed in DDD/100 discharges, was identified for cephalosporins -6.0% (p < 0.0001) and macrolides -16.5% (p < 0.0001). Macrolides also showed a downward trend in consumption, as expressed in DDD/100 bed days (-14.3%, p < 0.0001). According to World Health Organization (WHO) access, watch and reserve (AWaRe) classification trend analysis, only the reserve group showed a statistically significant upward change of 9.2% (p = 0.016). Conclusions: This five-year analysis demonstrated trends over time in overall antimicrobial consumption measured in DDD/100 bed days and DDD/100 discharge rates that correlate. In general, for all antimicrobials, after the implementation of antimicrobial stewardship programs (ASP), a downward trend is reported; in contrast, during the COVID-19 pandemic the AMC shows a general upward trend. The comparison between DDD/100 bed days and DDD/100 discharges allows for complementary comparisons to be made regarding antimicrobial exposure in a clinical setting.

Assessing the Reliability, Dimensions, and Variance of Young's Internet Addiction Test by Applying it to Adolescents at the National Psychiatric Hospital in Costa Rica.

The Internet Addiction Test (IAT) was the first instrument created specifically to assess Internet addiction (IA). Although it was not validated during its creation, its validity has since been confirmed thanks to subsequent studies. The IAT is the first global psychometric measure tested worldwide that has been used in several studies as a research tool. Regarding the dimensions of the IAT, they were not reported by the creator of the test, during the performed factor analysis. Nonetheless, different studies have defined the test as being one-dimensional and others as having three, four, five, and six dimensions. In the present study, seven dimensions were identified; however, there is still a lack of consensus about the number of dimensions that accurately define this test. Hence, Young's Internet Addiction Test presents a shortcoming that may be explained by the influence of several factors such as how it was applied, the place where it was applied, and the population to which it was applied. All of these are factors that could be linked to the sociocultural aspects of the adaptations that have been made to the test. Moreover, the first dimension of the IAT found, the loss of self-control, is extremely important because it explains 34% of the variance of the data. However, 74% of the variance is explained by applying the 7 dimensions identified in this research. Also, the reliability analysis showed that the IAT is 89% reliable, which indicates that the elements comprising the test used in this research are suitable for measuring the construct of Internet addiction.

Variability in treatment approaches, outcomes, and recurrence patterns after curative intent treatment for primary retroperitoneal sarcomas in Latin America: A report on 333 patients from LATAMSARC collaborative working group

Background and objectiveRetroperitoneal sarcomas (RPS) are rare tumors with several well-defined histologic subtypes. This study aimed to assess the epidemiology, treatment patterns, and outcomes of patients with retroperitoneal sarcoma in Latin America and analyze patterns of recurrence and treatment variations in a large population of patients treated at reference centers. MethodsThis retrospective descriptive study utilized an electronic database of medical records from LATAMSARC (a group studying sarcomas consisting of different centers in various countries in Latin America, including Argentina, Chile, Costa Rica, Mexico, and Peru). Adult patients (≥18 years) with retroperitoneal sarcoma who underwent surgery at six cancer centers in Latin America were included. Key outcomes were practice patterns, overall survival (OS), and progression-free survival (PFS). ResultsIn this retrospective study, 333 patients diagnosed with retroperitoneal sarcomas met the inclusion/exclusion criteria. The cohort consisted of 157 females (42.3 %) and 176 males (57.7 %), with an average age of 53.9 years (SD 13.37). Histology most commonly included Dedifferentiated Liposarcoma (31.5 %), Well-Differentiated Liposarcoma (29.7 %), and Leiomyosarcoma (9.9 %). The tumors predominantly resided in the retroperitoneum (81.7 %) and showed a mean diameter of 23.53 cm (SD 13.8 cm). Surgical interventions varied, with Complete Compartmental Resection performed in 31.2 % of cases and Simple Complete Resection in 29.4 %. Postoperative complications were documented, with Clavien-Dindo Grade ≥3 complications occurring in 16.8 % of patients. An R0/1 resection was obtained in 91.6 % of cases. The ICU admission rate post-surgery was 18.6 %. Adjuvant treatments included radiotherapy in 8.1 %. Local recurrence occurred in 20.7 % of cases, with the majority being ipsilateral. Distal recurrence was observed in 15 patients, predominantly affecting the liver. The mean overall survival was 65.5 months, and disease-free survival was 47.0 months. ConclusionThis study provides information on the epidemiology, treatment patterns, and outcomes of retroperitoneal sarcomas in a group of reference centers in Latin America. These results represent Latin America's largest body of evidence on retroperitoneal sarcomas.

Assessing the Reliability, Dimensions, and Variance of Young's Internet Addiction Test by Applying it to Adolescents at the National Psychiatric Hospital in Costa Rica

Background: The Internet Addiction Test (IAT) was the first instrument created specifically to assess Internet addiction (IA). Although it was not validated during its creation, its validity has since been confirmed thanks to subsequent studies. The IAT is the first global psychometric measure tested worldwide that has been used in several studies as a research tool. Regarding the dimensions of the IAT, they were not reported by the creator of the test, during the performed factor analysis. Nonetheless, different studies have defined the test as being one-dimensional and others as having three, four, five, and six dimensions. Methods: In the present study, seven dimensions were identified; however, there is still a lack of consensus about the number of dimensions that accurately define this test. Results: Hence, Young's Internet Addiction Test presents a shortcoming that may be explained by the influence of several factors such as how it was applied, the place where it was applied, and the population to which it was applied. All of these are factors that could be linked to the sociocultural aspects of the adaptations that have been made to the test. Conclusions: Moreover, the first dimension of the IAT found, the loss of self-control, is extremely important because it explains 34% of the variance of the data. However, 74% of the variance is explained by applying the 7 dimensions identified in this research. Also, the reliability analysis showed that the IAT is 89% reliable, which indicates that the elements comprising the test used in this research are suitable for measuring the construct of Internet addiction.

Decision making in managing semiotic conflicts in curriculum materials: a training program with prospective teachers in Costa Rica

Decision making in managing semiotic conflicts in curriculum materials: a training program with prospective teachers in Costa Rica

A New Endogean, Dwarf, and Troglomorphic Species of Swamp Eel of the Genus Ophisternon (Synbranchiformes: Synbranchidae) from Costa Rica: Evidence from Comparative Mitogenomic and Anatomical Data

A New Endogean, Dwarf, and Troglomorphic Species of Swamp Eel of the Genus Ophisternon (Synbranchiformes: Synbranchidae) from Costa Rica: Evidence from Comparative Mitogenomic and Anatomical Data

Mantled howler monkeys (Alouatta palliata) alter activity and spatial cohesion across a continuous forest and forest fragment in Costa Rica.

Habitat loss due to deforestation is a primary threat to global biodiversity. Clearing tropical rainforests for agriculture or development leads to forest fragmentation. Forest fragments contain fewer large trees and provide lower food availability for primates compared to continuous forests. Mantled howler monkeys (Alouatta palliata) inhabit fragmented rainforests and may need to alter their activity budgets and spatial cohesion to mitigate competition and conserve energy in fragments where food quality is lower. We compared howler monkey activity and spatial cohesion across a small forest fragment (La Suerte Biological Research Station, LSBRS) and a large, continuous forest (La Selva Research Station) in Costa Rica. We predicted that monkeys at LSBRS would rest more, feed more, travel less, and be less spatially cohesive compared to La Selva to contend with fewer resources in the small fragment. Using instantaneous scan sampling at 2-min intervals during 30-min focal samples, we recorded activity and the number of individuals within 5m of the focal animal. We collected 1505h of data from 2017-2024. Monkey activity and spatial cohesion differed significantly across sites. As predicted, monkeys at LSBRS spent more time feeding than at La Selva, but contrary to our predictions, they rested less and traveled more. The mean number of individuals within 5m was significantly lower at LSBRS compared to La Selva. The ability to modify their activity and spatial cohesion in response to fragmentation provides insight into how primates can contend with fewer resources and higher competition in changing ecosystems worldwide.

Tracing Urban Drinking Water Sources: Global State of the Art and Insights From an IAEA‐Coordinated Research Project

ABSTRACTClimate change, inter‐annual precipitation variability, recurrent droughts and flash flooding, coupled with increasing water needs, are shaping the co‐evolution of socioeconomic and cultural assemblages, water laws and regulations, and equitable drinking water access and allocation worldwide. Recognising the need for mitigation strategies for drinking water availability in urban areas, the Isotope Hydrology Section of the International Atomic Energy Agency (IAEA) coordinated a state‐of‐the‐art global assessment to evaluate water sources and distribution of drinking water supply in urban centres, an initiative entitled ‘Use of Isotope Techniques for the Evaluation of Water Sources for Domestic Supply in Urban Areas (2018–2023)’. Here, we report on (a) current research trends for studying urban drinking water systems during the last two decades and (b) the development, testing and integration of new methodologies, aiming for a better assessment, mapping and management of water resources used for drinking water supply in urban settings. Selected examples of water isotope applications (Canada, USA, Costa Rica, Ecuador, Morocco, Botswana, Romania, Slovenia, India and Nepal) provide context to the insights and recommendations reported and highlight the versatility of water isotopes to underpin seasonal and temporal variations across various environmental and climate scenarios. The study revealed that urban areas depend on a large spectrum of water recharge across mountain ranges, extensive local groundwater extraction and water transfer from nearby or distant river basins. The latter is reflected in the spatial isotope snapshot variability. High‐resolution monitoring (hourly and sub‐hourly) isotope sampling revealed large diurnal variations in the wet tropics (Costa Rica) (up to 1.5‰ in δ18O) and more uniform diurnal variations in urban centres fed by groundwater sources (0.08‰ in δ18O) (Ljubljana, Slovenia). Similarly, while d‐excess was fairly close to the global mean value (+10‰) across all urban centres (10‰–15‰), reservoir‐based drinking water systems show lower values (up to ~ −20‰) (Arlington, TX, USA and Gaborone, Botswana), as a result of strong evapoconcentration processes. δ18O time series and depth‐integrated sampling highlighted the influence of the catchment damping ratio in the ultimate intake water composition. By introducing new, traceable spatial and temporal tools that span from the water source to the end‐user and are linked to the engineered and socio‐economic structure of the water distribution system, governmental, regional or community‐based water operators and practitioners could enhance drinking water treatment strategies (including more accurate surface water blending estimations) and improve urban water management and conservation plans in the light of global warming.

Landslides and flood hazard mapping using geomorphological methods in Santa Ana, Costa Rica

This study addresses the critical need for comprehensive hazard maps to guide urban and periurban risk management in Santa Ana, Costa Rica. Located near the capital, San Jose, Santa Ana is a rapidly developing municipality characterized by high-value properties, commercial zones, luxury housing, agricultural, and protected areas. However, it faces significant challenges from landslides and floods. Despite its vulnerability, the municipality lacks detailed, holistic hazard assessments. To bridge this gap, we employed geomorphological methods, including morphometry and morphogenetics, integrated with geographic information systems (GIS), historical data, and on-site validation. Our analysis identified that slopes exceeding 20° are particularly susceptible to landslides, with valleys and unstable hillslopes being critical zones for seismic and water-induced destabilization. For flood hazards, areas with low slope and high flow accumulation, such as alluvial fans and flood plains, were found to be highly susceptible. Field and mapping validation against the DesInventar disaster database confirmed the accuracy of our hazard zones. These findings provide essential geospatial insights for effective risk management, decision-making, and territorial planning, enabling proactive and adaptive responses to natural hazards in Santa Ana. While this study does not present radical methodological innovations, its main contribution lies in demonstrating how easy-access and practical geomorphological tools can be used to generate valuable information on risk zoning in regions with limited resources. Furthermore, the methodology and insights from this study are applicable to other urban and periurban regions facing similar geomorphic hazards, highlighting its broader relevance.

Stress levels, hematological condition, and productivity of plasma-producing horses used for snake antivenom manufacture: A comparison of two industrial bleeding methods

The immunization and industrial bleeding of horses are essential stages for producing snake antivenoms. In Costa Rica, the traditional method involves stimulating the antibody response of horses by periodically injecting venoms, collecting hyperimmune plasma over three consecutive bleeding days, and repeating this process every eight weeks. While this method does not cause major physical or hematological issues in horses, the associated stress has not been evaluated. We compared this traditional method with an alternative method that involves injecting venoms, collecting hyperimmune plasma in a single bleeding day, and repeating the process every two weeks. We assessed stress (via serum and fecal cortisol levels and an ethological study), hematological parameters (hematocrit and hemoglobin concentration), and plasma productivity over eight months. Serum cortisol levels remained within the normal range for both methods throughout the immunization/bleeding cycle. However, serum and fecal cortisol levels were significantly higher in horses subjected to the traditional method compared to those in the alternative method. Neither method caused significant hematological alterations. Notably, the alternative method yielded a higher volume of plasma. We concluded that adopting the alternative method ensures horse welfare while improving industrial bleeding productivity. This approach may reduce costs and improve the availability of this essential treatment for vulnerable populations.

Between Heterogeneity and Professionalization of TVET Teacher Education in Costa Rica

Between Heterogeneity and Professionalization of TVET Teacher Education in Costa Rica

Políticas de liderazgo y gestión en instituciones de educación superior de México y Costa Rica

Educational policies focused on management and leadership in higher education have gained significant importance in recent years. Decision-making, planning, organizing, and executing strategies are the responsibility of the leadership of schools, units, and faculties within various university bodies. The knowledge, skills, and competencies in management and leadership of these leaders are essential elements for implementing development strategies for research, teaching, and outreach. This study aims to compare educational leadership policies in higher education in Mexico and Costa Rica, focusing on regulations, policies, and professional development at the University of Guadalajara, the National University of Costa Rica, and the University of Costa Rica, as well as to identify a binational model. The research was conducted from a naturalistic approach with a comparative methodology, using the case study technique through document analysis. The findings conclude that the three universities are characterized by a diversity of styles and approaches in their policies, regulations, training programs, and curricula to address management and leadership, ranging from more traditional models to more participatory and collaborative approaches. They address university organization indifferent and varied ways, each with a specific logic. It was not possible to determine a binational university leadership model, nor are there institutional policies directly aimed at management and leadership for university authorities, thus opening a research avenue for future academic and interinstitutional practice.