Cornus Sericea Research Articles

Evaluating the effects of red osier dogwood extract and grape pomace on growth performance, plasma biochemistry, intestinal morphology and microbiota, and immune and antioxidant status of broiler chickens challenged with heat stress

288 one-day-old Ross 308 broiler chicks were randomly allotted into 4 dietary treatments; 1)Control (CON; corn-wheat-soybean based diet), 2)CON + 0.05% bacitracin methylene disalicylate (BMD), 3)CON + 0.3% ROD, and 4)CON + 2.5% GP and raised for 28 d. On d 21-27, birds were exposed to either a thermoneutral (TN; 24°C±1) or heat stress (HS; 32-34°C for 8 hr/day). On d 28, twelve male birds per treatment/temperature group were euthanized for measuring blood and intestinal health parameters. 0.05% BMD and 0.3% ROD inclusion improved (P ≤ 0.05) the ABWG and FCR, compared to CON. HS reduced (P ≤ 0.05) AFI, bursa and spleen weights, plasma ALT, lipase, and CK but increased (P ≤ 0.05) ALP, serum immunoglobulins compared to TN birds. HS further reduced (P ≤ 0.05) duodenal VH:CD, jejunal VH and VH:CD but this was reversed by ROD and GP. 0.05% BMD and 2.5% GP increased (P < 0.001) the relative abundance of Blautia and Sellimonas compared to the CON diet. ROD increased (P = 0.010) the relative abundance of Faecalibacterium compared to the BMD diet. The results suggest that 0.3% ROD extract may function as an alternative to antibiotics in improving broiler growth performance and intestinal health.

Effect of red osier dogwood extract on in vitro gas production, dry matter digestibility, and fermentation characteristics of forage-based diet or grain-based diet

This in vitro batch culture study investigated the effects of red osier dogwood (ROD) extract supplementation on gas production (GP), dry matter disappearance (DMD), and fermentation characteristics in high forage (HF) and high grain (HG) diets with varying media pH level. The experiment was a factorial arrangement of treatments in a completely randomized design with 2 media pH (5.8 and 6.5) × 4 dose rates of ROD extract (0, 1, 3, and 5% of DM substrate). An additional treatment of monensin was added as a positive control for each pH level. The HF substrate consisted of 400 and 600 g/kg DM barley-based concentrate and barley silage, respectively, while the HG substrate contained 100 and 900 g/kg DM barley silage and barley-based concentrate, respectively. Treatments were incubated for 24 h with GP, DMD and fermentation parameters determined. No interaction was detected between the media pH level and ROD extract dose rate on GP, DMD and most of the fermentation parameters. The GP, DMD, and total volatile fatty acid (VFA) concentration were greater (P = 0.01) with media pH of 6.5 in both HF and HG diets. The GP were not affected by increasing ROD dose rate, except that GP linearly decreased in the HF (P = 0.04) and HG (P = 0.01) diets at 24 h; the DMD tended to linearly decrease at pH 6.5 (P = 0.06) for both HF and HG diets and at pH 5.8 (P = 0.02) for the HG diet. Adding ROD extract to the HF and HG diets linearly (P = 0.01) increased the acetate molar proportion at high or low media pH and consequently, the acetate to propionate (A:P) ratio linearly (P ≤ 0.04) increased. Supplementation of ROD extract to the HF diet linearly (P = 0.04) decreased the molar proportion of propionate at pH 6.5 (interaction between pH and ROD extract; P = 0.05), but had no effect on propionate proportion when added to the HG diet. Moreover, the proportion of branched-chain fatty acids linearly (P = 0.03) decreased with ROD extract supplementation at low pH (interaction, P < 0.05) for HF diet and linearly decreased (P = 0.05) at pH 6.5 for HG diet (interaction, P < 0.05). The NH3–N concentration was not affected by ROD supplementation in the HF diet but it linearly (P = 0.01) decreased with increasing dose rate in the HG diet. Methane concentration tended to linearly (P = 0.06) increase with ROD extract supplementation at high pH for HF diet and linearly increased at pH 5.8 (P = 0.06) and pH 6.5 (P = 0.02) for HG diet. These results indicate that the decreased DMD and increased A:P ratio observed with addition of ROD extract may be beneficial to HG-fed cattle to reduce the risk of rumen acidosis without negatively impacting fiber digestion.

Impact of de-icing salt runoff in spring on bioretention efficiency

Abstract We investigated the effect of de-icing salt in stormwater runoff on bioretention system hydrology and filtration of contaminants. Salt runoffs during the snow melt period were simulated in 20 mesocosms planted with 1 of 3 plant species (Cornus sericea, Juncus effusus and Iris versicolor) or left unplanted, and then watered with semi-synthetic stormwater runoffs supplemented with 4 NaCl concentrations (0, 250, 1,000 or 4,000 mg Cl/L). All bioretention mesocosms, irrespective of treatment, were efficient in reducing water volume, flow and pollution level. There was no phytotoxic effect of NaCl on plants, even at the highest NaCl concentration tested. Water volume reduction and flow rate were influenced by plant species, but salt concentration had no effect. Salt runoffs significantly increased the removal of some metals, such as Cr, Ni, Pb and Zn, but had no effect on nutrient removal. Because snowmelt laden with de-icing salt is of short duration and occurs during plant dormancy, plants in bioretention may be less affected by de-icing salt than previously thought, provided that salinity decreases rapidly to normal levels in the soil water. The long-term effects of de-icing salt and general performance of bioretention should be further studied under full-scale conditions.

PSXIII-13 Effect of Supplementation of Red Osier Dogwood Extract on Feed Intake and Growth Performance of Newly Weaned Beef Calves

Abstract Red osier dogwood (ROD) is a Canadian native shrub plant that is rich in phenolic compounds with antimicrobial and antioxidant properties. The high phenolic compounds with antimicrobial and antioxidant properties would be beneficial to reduce oxidative stress, hence improve animal growth performance when animals are stressed. The objective of this study was to evaluate the effects of feeding ROD extract and the residue after extraction to newly weaned calves on feed intake and growth performance. Eighty crossbred Angus steers [initial body weight (BW), 254 ± 5.4 kg] were used and housed in individual feedlot pen. The experiment was a randomized complete block design in five treatments (16 steers/treatment). Five treatments were: 1) Control (without ROD); 2) 20 g/d ROD extract (RODLow); 3) 40 g/d ROD extract (RODHigh); 4) 100 g/d ROD residue (RODRes); and 5) 330 mg monensin/d (MON). The MON was used as positive control because it is commonly fed to feedlot cattle to improve feed efficiency and animal health. The experiment diet consisted of 60% silage and 40% concentrate (DM basis) and fed for 84 d. The DPPH free radical scavenging activity of ROD extracts and the residue after extraction were determined. The high DPPH scavenging was observed for both ROD extracts (60.6 ± 4.7%) and residue (80.6 ± 1.7%) at the concentration of 0.5 mg/mL. Dry matter (DM) intake (kg/d) overall did not differ between control (6.44) and ROD supplemented calves (average, 6.27), whereas the DM intake tended (P = 0.10) to be greater with ROD extract (4.97 kg/d) than control (4.56 kg/d) at the first 28 d. The final BW averaged daily gain and feed efficiency were not affected by increasing dose of ROD extract (0, 20, 40 g/d) or by adding 100 g/d ROD residue. The calves fed MON had reduced (P = 0.04) DM intake (5.72 vs. 6.32 kg/d) and decreased (P = 0.07) daily gain (0.81 vs. 0.94 kg/d) than other treatments. Fecal pH ranged from 6.98 to 6.17 and did not differ among treatments. These results indicate that feeding ROD extract or its residue to newly weaned calves may increase feed intake when the animals are stressed; however, its effect on growth performance and feed efficiency is not apparent under current feeding conditions.

Growth and survival of native upland and wetland species in shallow capped thickened tailings: a meso-scale greenhouse study

This 3 year meso-scale greenhouse study used 55 gallon columns to evaluate the survival and growth of boreal upland and wetland communities on thickened tailings (TT) with 0 cm, 10 cm, and 30 cm peat mineral mix (PMM) reclamation cap. While survival was high in all treatments, the PMM cap treatments showed significant improvement in overall plant growth, cover, and above-ground biomass compared to the uncapped treatment, with growth on the 30 cm PMM cap outperforming the 10 cm PMM cap. The plant growth response was similar between the two communities and the top performing species, in terms of survival and growth, in capped TT were Cornus sericea, Populus tremuloides, Salix bebbiana, and Scirpus microcarpus.

Red osier dogwood and its use in animal nutrition: A review.

As the human population increases globally, the food animal industry has not been spared from the monumental demand for edible animal products, particularly meat. This has necessitated the simultaneous expansion of the productivity of the animal sector to meet the ever-growing human needs. Although antibiotics have been used in food animal production with commendable positive impacts on their growth performance, their sole contributive factor to the increasing incidence of antimicrobial resistance has ushered the strict restrictions placed on their use in the animal sector. This has handed a setback to both animals and farmers; thus, the intense push for a more sustainable antibiotic alternative for use in animal production. The use of plants with concentrated phytogenic compounds has gained much interest due to their beneficial bioactivities, including antioxidant and selective antimicrobial. While the reported beneficial activities of phytogenic additives on animals vary due to their varying total polyphenol concentrations (TPC), red osier dogwood (ROD) plant materials boast of high TPC with excellent antioxidant prowess and growth improvement capacities compared to some plant extracts commonly used in research. However, its adoption in research and commercial scale is still low. Thus, the present review aims to provide concise information on the dietary potential of ROD plant materials in animal feeding.

Red osier dogwood extract versus Trimethoprim-sulfadiazine (Part 1). Effects on the growth performance, blood parameters, gut histomorphometry, and Salmonella excretion of broiler chickens orally challenged with Salmonella Enteritidis

The poultry industry has not been spared from the prevalent incidence of diseases caused by invasive pathogens, especially Salmonella. Due to the pressing need to identify a suitable antibiotic alternative for use in poultry production, this study investigated the efficacy of red osier dogwood (ROD) extract on the growth, blood parameters, gut morphology, and Salmonella excretion in broiler chickens orally challenged with Salmonella Enteritidis (SE). A 4×2 factorial experiment was conducted based on 2 main factors, namely dietary treatments, and SE challenge. A total of 404, one-day-old male Ross broiler chicks were randomly assigned to 4 dietary treatments; 1) Negative control (NC), 2) NC+0.075 ppm of Trimethoprim-sulfadiazine (TMP/SDZ)/kg of diet, 3) NC+0.3% ROD extract, and 4) NC+0.5% ROD extract. The absence of SE in the fecal samples obtained from chick delivery boxes was confirmed on d 0. On d 1, half of the birds were orally gavaged with 0.5 mL of phosphate-buffered saline each (noninfected group) and the remaining with 0.5 mL of 3.1×105 CFU/mL SE (infected group) in all treatment groups. Dietary treatments were randomly assigned to 8 replicate cages at 6 birds/cage. On 1-, 5-, 12-, and 18-day postinfection (DPI), cloacal fecal samples were collected on the 6 birds/cage to assess SE excretion. Average weight gain (AWG), average feed intake (AFI), feed conversion ratio (FCR), and mortality were determined weekly. On d 21, 10 chickens/treatment were euthanized to perform hematology, gut histomorphometry, serum immunoglobulins G and M (IgG and IgM), and superoxide dismutase measurements. Both ROD extract levels did not affect (P > 0.05) growth performance; however, the SE-infected birds showed increased (P < 0.05) AFI and FCR throughout the experimental period. Regardless of the SE-infection, both ROD extract levels improved (P < 0.05) duodenal villus height: crypt depth compared to other treatments. 0.5% ROD extract improved (P < 0.05) ileal villus width (VW) of noninfected birds and ileal crypt depth of infected birds, but it decreased (P < 0.05) the ileal VW of infected birds, compared to other treatments. The SE-infected birds showed lower (P < 0.05) lymphocytes (L) but increased (P < 0.05) heterophils (H), H:L, and monocytes (MON). Both ROD extract levels did not affect (P > 0.05) white blood cell differential, while dietary 0.3% ROD extract increased (P < 0.05) MON of the birds, regardless of infection model. Regardless of infection model, both TMP/SDZ and 0.5% ROD extract reduced the concentration of IgM in the serum, compared to the control and 0.3% ROD (P=0.006). Conclusively, both ROD extract levels improved duodenal histomorphology and body defense against SE infection in broiler chickens; however, the 0.3% ROD extract was better.

Red osier dogwood extract vs. trimethoprim-sulfadiazine (Part 2). Pharmacodynamic effects on ileal and cecal microbiota of broiler chickens challenged orally with Salmonella Enteritidis

With the subsisting restrictions on the use of antibiotics in poultry production, the use of plant extracts has shown some promising antimicrobial capacity similar to antibiotics; however, such capacity is largely dependent on their total polyphenol concentration and profile. Given the emerging antimicrobial potential of red osier dogwood (ROD) extract, the study aimed to investigate the pharmacodynamic effect of ROD extract on the ileal and cecal microbiota of broiler chickens challenged orally with Salmonella Enteritidis (SE). A 21 d 4×2 factorial experiment was conducted based on 2 main factors, including diets and SE challenge. A total of 384 one-day-old mixed-sex Cobb-500 broiler chicks were randomly allotted to 4 dietary treatments; Negative control (NC), NC+0.075 mg trimethoprim-sulfadiazine (TMP/SDZ)/kg of diet, and NC containing either 0.3 or 0.5% ROD extract. On d 1, half of the birds were orally challenged with 0.5 mL of phosphate-buffered saline (Noninfected group) and the remaining half with 0.5 mL of 3.1×105 CFU/mL SE (Infected group). Dietary treatments were randomly assigned to 8 replicate cages at 6 birds/cage. On d 21, 10 birds/treatment were euthanized and eviscerated to collect ileal and cecal digesta for gut microbiota analysis. The ileal and cecal microbiota was dominated by phyla Firmicutes, Proteobacteria, and Actinobacteriota. The SE infection decreased (P < 0.05) the relative abundance of Proteobacteria and Actinobacteriota in the ileum and ceca, respectively, however, it increased (P < 0.05) Proteobacteria in the ceca. Both 0.3 and 0.5% ROD extracts (P < 0.05) depressed the relative abundance of Actinobacteriota in the ileum but marginally improved (P < 0.05) it in the ceca compared to the TMP/SDZ treatment. Dietary TMP/SDZ increased (P < 0.05) genus Bifidobacterium at the ileal and cecal segments compared to other treatments. Dietary 0.3 and 0.5% marginally improved (P < 0.05) Bifidobacterium in the ceca and depressed (P < 0.05) Weissella and was comparably similar to TMP/SDZ in the ileum. Regardless of the dietary treatments and SE infection, alpha diversity differed (P < 0.05) between ileal and cecal microbiota. Beta diversity was distinct (P < 0.05) in both ileal and cecal digesta along the SE infection model. Conclusively, both ROD extract levels yielded a pharmacodynamic effect similar to antibiotics on ileal and cecal microbiota.

Plant species contribution to bioretention performance under a temperate climate

Bioretention systems are green infrastructures increasingly used to manage urban stormwater runoff. Plants are an essential component of bioretention, improving water quality and reducing runoff volume and peak flows. However, there is little evidence on how this contribution varies between species, especially in temperate climates with seasonal variations and plant dormancy. The aim of our study was to compare the performance of four plant species for bioretention effectiveness during the growing and dormant periods in a mesocosm study. The species selected (Cornus sericea, Juncus effusus, Iris versicolor, Sesleria autumnalis) are commonly used in bioretention and cover a wide range of biological forms and functional traits.All bioretention mesocosms were effective in reducing water volume, flow and pollutant levels in both of the studied periods. Plants decreased runoff volume and increased contaminant retention by reducing water flow (up to 2.7 times compared to unplanted systems) and increasing water loss through evapotranspiration during the growing period (up to 2.5 times). Plants improved removal of macronutrients, with an average mass removal of 55 % for TN, 81 % for TP and 61 % for K compared to −6 % (release), 61 % and 22 % respectively for the unplanted systems. Except for Sesleria, mass removal of trace elements in planted mesocosms was generally higher than in unplanted ones (up to 8.7 %), regardless of season. Between-species differences in exfiltration rate and improved water quality followed the same order as their evapotranspiration rate and overall size, measured in terms of plant volume, leaf biomass, total leaf area and maximum average root density (Cornus > Juncus > Iris > Sesleria). By increasing evapotranspiration, plants decreased runoff volume and increased contaminant retention. Nutrient removal was partly explained by plant assimilation. Our study confirms the importance of plant species selection for improving water quality and reducing runoff volume during bioretention under a temperate climate.

Potential lasting impacts of industrial herbicides on ungulate nutrition

Glyphosate-based herbicides (GBH) are an effective, yet controversial, tool for reducing the survival and growth of deciduous vegetation in commercial tree plantations. While glyphosate residues can be stored in plants for over 10 years, the nutritional impacts of GBH for ungulate forage are unknown. Here we compare the energy and protein content of moose ( Alces alces) food plants in central British Columbia, Canada. Willow ( Salix bebbiana), dogwood ( Cornus sericea), and fireweed ( Chamaenerion angustifolium) samples were obtained from regenerating cut blocks 1, 3, 6, and 12 years after GBH treatment and from untreated controls with identical initiation dates. We predicted that surviving plants would exhibit reduced palatability, digestibility, and nutritional value compared to controls, and that these effects would last at least 1 year before dissipating. Contrary to predictions, concentrations of digestible protein were higher in treated blocks 1 year after herbicide application, but in subsequent years there were few significant differences in protein from treated versus untreated forage. Digestible energy concentrations were identical to controls 1 year after exposure, but significant reductions were observed after 12 years. Results indicate potential nonlinear, complex, and long-lasting effects of GBH on the constituents of understory plants with implications for ungulate forage quality.

Effects of indole-3-butyric acid and age of stem cuttings on root morphology, growth, and survival of Cornus sericea

Effects of indole-3-butyric acid and age of stem cuttings on root morphology, growth, and survival of Cornus sericea

Effects of mycorrhizae, plants, and soils on phosphorus leaching and plant uptake: Lessons learned from a mesocosm study

Societal Impact StatementWorldwide, farmers struggle to find the most efficacious practices which balance crop fertility needs and water quality protection. Through a greenhouse experiment, we investigated how soil status (high vs. low phosphorus [P] concentration), mycorrhizae (inoculated vs. not), and plant species (dogwood vs. willow) affected P plant uptake and leaching. We found mycorrhizae did not affect uptake or leaching, more P was leached from high than low P soil, dogwood uptook yet leached more P, and above ground biomass at the end of summer contained more P than roots. This study provides insights to be considered by researchers and practitioners who implement best management practices for water quality.Summary This research examined the effects of mycorrhizal inoculation in high and low phosphorus saturation soils on phosphorus uptake by Cornus sericea and Salix niger. The aim was to identify practices that improved water quality functions of riparian buffers to protect surface waters impacted by eutrophication. A mesocosm experiment arranged as a random block design was conducted with mycorrhizal presence, soil phosphorus saturation status, and plant species as factors. Leachate, plant uptake, and soil phosphorus were measured to assess the effects. Greater leachate and uptake of phosphorus were detected for C. sericea than for S. niger. Mycorrhizae had no effects on leaching nor on uptake of phosphorus in this experiment. High phosphorus saturated soils had greater leaching and uptake than the low phosphorus soils. Above ground biomass contained more phosphorus than below ground biomass in both species at time of harvest. Estimations of phosphorus removal through coppicing suggest a very slow removal rate in biodiverse multi‐functional riparian buffers. Our results suggest that cyclical coppicing can be an improvement to Best Management Practices. Diverse riparian buffers are limited in the amount of phosphorus that they can store and mitigate, even with coppicing. The emphasis therefore should be on agricultural best management practices that reduce phosphorus export from upland fields. Further studies in phosphorus accumulating plant species with appropriate mycorrhizal symbionts are needed.

Effect of red osier dogwood extract on growth performance, blood biochemical parameters, and gut functionality of broiler chickens challenged or unchallenged intraperitoneally with Salmonella Enteritidis lipopolysaccharide

As we advance in the search for antibiotic-alternatives, harnessing plant materials with high total polyphenol concentration (TPC) would be quintessential. Given the high TPC in red osier dogwood (ROD) extract, the current study aimed to determine its efficacy on the growth performance, intestinal health, blood biochemistry, and antioxidant capacity of broiler chickens. A 21-day 4x2 factorial feeding trial was conducted based on two main factors namely, dietary treatments and Salmonella Enteritidis Lipopolysaccharides SE-LPS) challenge. A total of 384 one-day-old mixed-sex Cobb-500 broiler chicks were randomly allotted to four dietary treatments - Negative control (NC), NC + 0.05% bacitracin methylene disalicylate (BMD), NC + 0.3%ROD, and NC+0.5% ROD. Each treatment was assigned to eight replicates with six birds/replicate. On d 13 and 20, half of the birds were intraperitoneally injected with 1mL phosphate-buffered-saline /kg BW of birds (Unchallenged-group) and the remaining half with 1mg SE-LPS /kg BW of birds (Challenged-group). Average weight gain (AWG), average feed intake (AFI), feed conversion ratio (FCR), and mortality were determined weekly. On d 21, ten chickens/treatment were euthanized for measuring blood biochemical parameters, immune organ weights, caecal SCFA, and caeca microbiota. The SE-LPS decreased (P < 0.05) AWG and FCR on d 14 and 21, respectively. On d 14, 21, and overall basis, both ROD extract levels marginally improved (P < 0.05) the AWG of unchallenged birds compared to other treatments in the unchallenged-group. Challenged and unchallenged birds fed ROD extract had deeper (P < 0.05) crypt depth (CD) and higher villus height:CD, respectively, in the ileum. Globulin (GLB) and albumin:GLB were increased and reduced (P < 0.05), respectively, among birds fed 0.3%ROD compared to other treatments. There was no treatment effect on caeca SCFA, relative weight of immune organs, and serum antioxidants. Birds fed ROD extract had a higher (P < 0.05) relative abundance of caecal Lactobacillus and Streptococcus genera compared to the antibiotic treatment. Conclusively, incorporating 0.3% and 0.5%ROD extract into broiler chickens' nutrition improved growth performance and ileal morphology, and modified caecal microbiota of broiler chickens, regardless of the intraperitoneal SE-LPS challenge.

Effect of Supplementing Moderate Grain or High-Grain Diets with Red Osier Dogwood Extract on in Vitro Gas Production, Feed Disappearance and Fermentation Characteristics

Effect of Supplementing Moderate Grain or High-Grain Diets with Red Osier Dogwood Extract on in Vitro Gas Production, Feed Disappearance and Fermentation Characteristics

Dietary supplementation of red-osier dogwood polyphenol extract changes the ileal microbiota structure and increases Lactobacillus in a pig model

Red-osier dogwood (ROD) extract contains a lot of polyphenols that have the potential for modulation of gut microbiota. However, little information is available about its prebiotic properties. This study investigated the impact of ROD polyphenol extract on the ileal microbiota with dietary supplementation of ROD polyphenol extract in a pig model. The data indicated that supplementation of ROD polyphenol extract significantly increased class Bacilli, order Lactobacillales and family lactobacillaceae. Within family lactobacillaceae, Lactobacillus was the main responder by increasing from 5.92% to 35.09%. Further analysis showed that ROD polyphenol extract improved two species Lactobacillus delbrueckii and Lactobacillus mucus. The results of this study suggested that ROD polyphenol extract has the potential to play prebiotic role and confer health benefit through modifying gut microbiota.

PSX-A-11 Late-Breaking: Effects of phytogenic supplementation on growth performance and intestinal barrier function in nursery pigs challenged with E. coli

Abstract The objective of this study was to investigate the health-promoting effects of red osier dogwood (ROD) extract as an alternative to antibiotics in weaned piglets challenged with enterotoxigenic Escherichia coli (ETEC). Twenty-eight weaned piglets (9.15±0.95 kg BW) confirmed to genetically susceptible to ETEC were individually assigned to one of the four dietary treatments in a completely randomized design. Experimental diets were, negative control (NC), corn-wheat soybean meal diet with no additives; positive control (PC), NC plus antibiotics; ROD1, NC plus 0.1% ROD extract; ROD2, NC plus 0.2% ROD extract. Piglets were orally challenged on d 7 with ETEC F4. Feed disappearance, body weight, fecal score, and rectal temperature were recorded. On d 14, piglets were euthanized to collect intestinal tissue samples for histomorphology and Ussing chamber analysis. Data were analyzed using the MIXED procedure of SAS using individual piglet as the experimental unit. There were no differences (P &amp;gt; 0.10) in histomorphology and intestinal permeability. Piglets fed the NC diet tended (P &amp;lt; 0.10) to have higher average daily gain, post-inoculation than those fed ROD1 or ROD2. Fecal score of piglets fed the PC diet tended to (P &amp;lt; 0.10) or was significantly lower (P &amp;lt; 0.05) than for piglets fed ROD1 or ROD2 on 0 and 2 days post-inoculation (dpi), respectively. On 0 dpi, piglets fed the ROD1 diet had significantly higher (P &amp;lt; 0.05) body temperature than those fed PC or ROD2. In conclusion, ROD extract supplementation might have some health-promoting effects on ETEC challenged piglets but could not improve gut health to the same extent as antibiotics.

PSXIV-14 Supplementation of high-grain diet with red osier dogwood affects in vitro digestibility and fermentation characteristics

Abstract Red osier dogwood (ROD) is a native shrub plant rich in phenolic compounds with antimicrobial properties. The objective of this study was to evaluate the effects of substituting barley silage with either raw ROD or ROD extract (RODE) in high-grain (HG) diet under a low media pH (5.8) on gas production (GP), dry matter (DM) disappearance (DMD) and fermentation characteristics in batch cultures. The study was a completely randomized design with 4 treatments: 1) control diet (10% barley silage and 90% barley concentrate, DM basis), control diet supplemented with 2) monensin (30 mg/kg diet DM; positive control), 3) substitution of 3% ROD or 4) 3% RODE for an equal portion of silage. Inoculum was obtained from 2 ruminally fistulated beef heifers offered the HG diet. Substrate ground (1 mm) was incubated for 24 h and the experiment repeated twice. The GP did not differ among treatments (147 ml/g DM), but the DMD differed (P &amp;lt; 0.02) at highest for control (69.4%), lowest for ROD (58.4%) and intermediate for other treatments (64.1%). Total volatile fatty acid (VFA) concentration (mM) tended (P&amp;lt; 0.08) to be lower with ROD (80.5) and monensin (80.1) than control (83.9). Acetate proportion was greater (P = 0.02) with ROD (46.2%) and RODE (46.9%) than control (42.4%) and monensin (42.3%). However, the propionate proportion was greater (P = 0.05) with monensin (32.1%) than other treatments (averaged 30.1%). Consequently, acetate to propionate ratio (A:P) of ROD (1.52) and RODE (1.56) was higher than monensin (1.32; P &amp;lt; 0.01) and control (1.44; P &amp;lt; 0.08). Differences in variables measured between ROD and RODE were minimal. These results indicated that the decreased DMD along with increased A:P with addition of ROD or RODE suggests that both ROD and RODE may be beneficial to HG fed cattle for reducing risk of rumen acidosis without negatively impacting fibre digestion.

PSX-B-8 Effect of supplementing red osier dogwood extract on in vitro gas production, feed digestibility and fermentation characteristics of high-forage diet

Abstract Red osier dogwood (ROD) is native shrub plant in Canada and rich in bioactive compounds. The objective of this study was to evaluate the effects of ROD extract supplementation in a high-forage (HF) diet on gas production (GP), dry matter (DM) disappearance (DMD) and fermentation characteristics in batch cultures with varying media pH. The study was a completely randomized design with 4 levels of ROD extract (0, 1, 3 and 5% of substrate) × 2 media pH (5.8 and 6.5) factorial arrangement. The study was conducted in three replicate experiments. Substrate contained 60% barley silage and 40% barley concentrate (DM basis). Inoculum was obtained from 2 ruminally fistulated beef heifers offered the HF diet. Substrate (0.5 g DM) ground (1 mm) was incubated for 48 h in a culture bottle. Data were analyzed using Mixed procedure of SAS with fixed effects of treatments and random effect of experiment. There was no interaction between media pH and level of ROD on GP, DMD and fermentation characteristics. Increased media pH (5.8 vs. 6.5) increased (P &amp;lt; 0.01) GP (averaged 164 vs. 275 ml/g substrate), DMD (50.6 vs. 60.6%), and total VFA production (63 vs. 71 mM). Increasing ROD extract levels did not affect GP but linearly (P = 0.05) decreased DMD from 62.1 to 58.9% at pH 6.5. Although total VFA production was not affected, increasing ROD extract linearly (P &amp;lt; 0.01) increased the proportion of acetate (47.9 to 49.7%) and propionate (20.5 to 22.3%) at pH 5.8; whereas at pH 6.5, acetate to propionate ratio quadratically (P = 0.04) changed to be lower with 3% ROD extract (2.08) than other treatments (2.21). These results indicated that supplementation of HF diet with ROD extract may improve fibre digestion at low media pH, and improve fermentation efficiency at high media pH.

Contrast in Post-Chill Eclosion Time Strategies Between Two Specialist Braconid Wasps (Hymenoptera: Braconidae) Attacking Rhagoletis Flies (Diptera: Tephritidae) in Western North America.

Parasitoids comprise a speciose insect group, displaying a wide array of life history strategies. In the Pacific Northwest of the United States, the tephritid fruit flies Rhagoletis tabellaria (Fitch) and Rhagoletis indifferens Curran infest red osier dogwood, Cornus sericea L. (Cornaceae), and bitter cherry, Prunus emarginata (Douglas ex Hooker) Eaton (Rosaceae), respectively. The flies are parasitized by different braconid wasps at different life stages; Utetes tabellariae (Fischer) oviposits into R. tabellaria eggs, whereas Diachasma muliebre (Muesebeck) oviposits into R. indifferens larvae feeding in cherries. Because Rhagoletis only have one major generation a year and the wasps attack temporally distinct fly life stages, we predicted that eclosion times of U. tabellariae should more closely follow that of its host than the larval-attacking D. muliebre. As predicted, U. tabellariae eclosed on average 6.0-12.5 d later than R. tabellaria, whereas D. muliebre eclosed on average 32.1 d after R. indifferens. Unexpectedly, however, longer chill duration differentially affected the systems; longer overwinters minimally influenced eclosion times of R. tabellaria and U. tabellariae but caused earlier eclosion of both R. indifferens and D. muliebre. Results imply that in temperate regions, diapause timing in braconid wasps evolves in response to both host life stage attacked and fly eclosion characteristics, possibly reflecting differential effects of winter on host plant fruiting phenology. Differences in phenological sensitivity of the lower host plant trophic level to variation in environmental conditions may have cascading effects, sequentially and differentially affecting eclosion times in higher frugivore (fly) and parasitoid (wasp) trophic levels.

Bark spectral signatures of one-year-old twigs of different shrubs mainly depend on their biochemical traits

The interaction of bark with light depends on the optical properties of the bark, which are important for plant energy balance especially out of the vegetation season. Light reflected from bark also represents a kind of “bark spectral signature” that may be species-specific. This study examines some morphological, biochemical, and physiological traits that may affect the reflectance of the bark of 1-year-old twigs of different shrubs: Corylus avellana, Rosa canina, Ligustrum vulgare, Sambucus nigra, Cornus sericea var. flaviramea, and Viburnum lantana. High variability was seen across these species for morphological, biochemical, and physiological traits, except for photochemical efficiency of photosystem II. The bark spectral signatures differed significantly across these species. The reflectance peak for C. sericea var. flaviramea was in red, for C. avellana in green, and the other species showed a wide peak from green to red light. Redundancy analysis revealed that UV-B–absorbing substances and anthocyanin content, along with outer and inner bark thickness, together explained 61% of the reflectance spectra variability. Outer bark thickness was important for reflectance in UV, violet, and blue, while anthocyanins were important for reflectance in green and yellow. Chlorophyll b was negatively related to the reflectance of visible light. This study revealed great importance of biochemical traits for explaining bark reflectance. Differences in “bark spectral signatures” enable differentiation across species out of the vegetation season.