Coprinus Comatus Research Articles

Enzyme inhibitory and antioxidant properties of six mushroom species from the Agaricaceae family

Mushrooms are excellent sources of nutraceuticals and have attracted much interest as functional foods. In this work the inhibitory capacity of six macrofungus (Coprinus comatus, Macrolepiota mastoidea, Agaricus campestris, Lycoperdon utriforme, Macrolepiota procera, and Leucoagaricus leucothites) against key enzymes related to diabetes type II (α-amylase, α-glucosidase), Alzheimer's disease [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] was assessed for the first time. The antioxidant properties (total antioxidant capacity, metal chelating, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assays) and phenolic contents of the selected mushroom species were also evaluated. We found that L. utriforme had the highest enzyme inhibitory activity (0.97mg galantamine equivalents (GALAE)/g extract, 1.33mg GALAE/g extract, 0.22mmol acarbose equivalents (ACAE)/g extract, and 2.97mmol ACAE/g extract against AChE, BChE, α-amylase, and α-glucosidase, respectively). A widely consumed mushroom species namely A. campestris possessed the highest phenolic content (15.63mg GAE/g extract) and the maximum antioxidant potential against DPPH, ABTS, FRAP, and CUPRAC. Experimental data collected in the present study support the use of these mushrooms specially L. utriforme and A. campestris as functional foods for the management and/or prevention of diabetes type II, Alzheimer's disease, and oxidative stress related complications.

First report of a cross-kingdom pathogenic bacterium, Achromobacter xylosoxidans isolated from stipe-rot Coprinus comatus

Coprinus comatus is an edible mushroom widely cultivated in China as a delicious food. Various diseases have occurred on C. comatus with the cultivated area increasing. In this study, the pathogenic bacterium JTG-B1, identified as Achromobacter xylosoxidans by 16S rDNA and nrdA gene sequencing, was isolated from edible mushroom Coprinus comatus with serious rot disease on its stipe. A. xylosoxidans has been confirmed as an important opportunistic human pathogenic bacterium and has been isolated from respiratory samples from cystic fibrosis. It is widely distributed in the environment. Here, we first report that fungi can also serve as a host for A. xylosoxidans. We confirmed that it can cross-kingdom infect between animals (mice) and fungi (C. comatus). The results of pathogenicity tests, physiological, biochemical and genotyping analysis of A. xylosoxidans from different hosts suggested that different strain of A. xylosoxidans may have pathogenicity differentiation. A. xylosoxidans not only is pathogenic to C. comatus but also may threaten human health.

Strengthening detoxication impacts of Coprinus comatus on nickel and fluoranthene co-contaminated soil by bacterial inoculation

To develop an efficient and environmental-friendly approach to detoxicate nickel (Ni) and fluoranthene co-contaminated soil, the combined application of Coprinus comatus (C. comatus) with Serratia sp. FFC5 and/or Enterobacter sp. E2 was investigated. The pot experiment tested the influences of bacterial inoculation on the growth of C. comatus, content of Ni in C. comatus, Ni speciation in soil, fluoranthene dissipation, soil enzymatic activities, bacterial population and community structure. With the inoculation of bacteria, the fresh weights of C. comatus, concentration of Ni in C. comatus and the dissipation rates of fluoranthene were increased by 17.73–29.38%, 68.97–204.97% and 34.84–60.90%, respectively. Notably, results illustrated that the co-inoculation of FFC5 and E2 showed better effect in biomass enhancement, Ni accumulation and fluoranthene dissipation than solitary inoculation. Simultaneously, higher soil enzymatic and microbiological activities suggested that the integrated detoxication method of bacteria and C. comatus could improve soil quality. Therefore, we can infer that bacterial inoculation strengthened detoxication effect of C. comatus in Ni-fluoranthene co-contaminated soil, indicating that the combined application of C. comatus and bacteria can be an efficient alternative for detoxicating Ni and fluoranthene co-contaminated soil.

Growth of Mycelium of Different Edible and Medicinal Mushrooms on Medium Supplemented with Digestate from AD Biogas Plant

Digestate is remaining material after anaerobic digestion (AD) of a biodegradable feedstock. The AD process produces two main products: digestate and biogas; digestate is considered as a waste; however, it found application in agriculture i.e. as a soil conditioner or cultivation substrate component. Digestate is rich in nitrogen and phosphorus and can be used as a substrate component for mycelial growth of mushrooms. Aim of the study was examination of agar media supplementation with digestate from AD of foodwaste material on mycelial growth of cultivated and medicinal mushrooms. Mycelial growth of four mushroom species was investigated: Coprinus comatus, Ganoderma lucidum; Agaricus subrufescens and Laetiporus sulphureus. First experiment investigated four mushroom species and 3 digestate-only based agar media with growing amount of digestate extract. Later fastest growing mushrooms from exp. 1, C. comatus and G. lucidum, was chosen, mycelial growth was performed on 2 digestate-only based agar media and 2 what/digestate extract based agar media. Wheat-based and manure-based agar media was used as control medium. Study confirmed positive effect of digestate from AD as an addition or main component of agar media culture for mushroom mycelium growth. The amount of digestate extract affected mycelial growth of investigated species. The best agar media was digestate-based only with 25 g of extract and wheat/digestate based (150/50 g extract). The fastest and the best growth were obtained for C. comatus. The residue from AD biogas production could have found additional application as media product for mushroom mycelia production and further as a component for mushroom cultivation substrate.

Mushroom diversity in the Biligiri rangana hills of Karnataka (India)

Evergreen forests of Biligiri rangana hills (BR hills) spread over an area of 540 sq. KM over eastern most edge of Western Ghats in Karnataka. Climatic conditions are more favorable for establishment of mushrooms and complete their life cycle. In this study, fourteen mushroom species were collected from BR hills region during monsoon (June through September 2013) with the help of Solega tribe inhabited the region since many years. Of the fourteen mushrooms, two mushrooms were identified as Ganoderma lucidum and Polyporous flabelliformis based on their phenotypic characters. The other 12 mushrooms were identified by ITS (Internal Transcribed spacer)region sequence homology as Termitomyces sp. (BRM-3)., Auricularia delicate (BRM-4), Termitomyces microcarpus (BRM-5), Amanita sp. (BRM-6), Podoscypha petalodes (BRM-7), Agaricaceae sp. (BRM-8), Macrolepiota sp.(BRM-9), Calvatia holothurioides (BRM-10), Gymnopillus crociphyllus (BRM-11), Coprinus comatus (BRM-12), Gyrodontium sacchari (BRM-13) and Clitocybeafffellea (BRM-14). Among the fourteen mushrooms, three species viz., Termito-myces species, Auricularia delicate and Termitomyces microcarpus were edible. The others were non edible/poisonous species. This study reports the diverse mushroom species as addition to biodiversity at BR hills.

Cytotoxic protein from the mushroom Coprinus comatus possesses a unique mode for glycan binding and specificity

Glycans possess significant chemical diversity; glycan binding proteins (GBPs) recognize specific glycans to translate their structures to functions in various physiological and pathological processes. Therefore, the discovery and characterization of novel GBPs and characterization of glycan-GBP interactions are significant to provide potential targets for therapeutic intervention of many diseases. Here, we report the biochemical, functional, and structural characterization of a 130-amino-acid protein, Y3, from the mushroom Coprinus comatus Biochemical studies of recombinant Y3 from a yeast expression system demonstrated the protein is a unique GBP. Additionally, we show that Y3 exhibits selective and potent cytotoxicity toward human T-cell leukemia Jurkat cells compared with a panel of cancer cell lines via inducing caspase-dependent apoptosis. Screening of a glycan array demonstrated GalNAcβ1-4(Fucα1-3)GlcNAc (LDNF) as a specific Y3-binding ligand. To provide a structural basis for function, the crystal structure was solved to a resolution of 1.2 Å, revealing a single-domain αβα-sandwich motif. Two monomers were dimerized to form a large 10-stranded, antiparallel β-sheet flanked by α-helices on each side, representing a unique oligomerization mode among GBPs. A large glycan binding pocket extends into the dimeric interface, and docking of LDNF identified key residues for glycan interactions. Disruption of residues predicted to be involved in LDNF/Y3 interactions resulted in the significant loss of binding to Jurkat T-cells and severely impaired their cytotoxicity. Collectively, these results demonstrate Y3 to be a GBP with selective cytotoxicity toward human T-cell leukemia cells and indicate its potential use in cancer diagnosis and treatment.

Combined bioremediation of soil co-contaminated with cadmium and endosulfan by Pleurotus eryngii and Coprinus comatus

The subjects of this study were to investigate the remediating potential of the co-cultivation of Pleurotus eryngii and Coprinus comatus on soil that is co-contaminated with heavy metal (cadmium (Cd)) and organic pollutant (endosulfan), and the effects of the co-cultivated mushrooms on soil biochemical indicators, such as laccase enzyme activity and bacterial counts. A pot experiment was conducted to investigate the combined bioremediation effects on co-contaminated soil. After the mature fruiting bodies were harvested from each pot, the biomass of mushrooms was recorded. In addition, bacterial counts and laccase enzyme activity in soil were determined. The content of Cd in mushrooms and soil was detected by the flame atomic absorption spectrometry (FAAS), and the variations of Cd fractions in soil were determined following the modified BCR sequential extraction procedure. Besides, the residual endosulfan in soil was detected by gas chromatography-mass spectrometry (GC-MS). The results indicated that co-cultivation of P. eryngii and C. comatus exerted the best remediation effect on the co-contaminated soil. The biomass of mushroom in the co-cultivated group (T group) was 1.57–13.20 and 19.75–56.64% higher than the group individually cultivated with P. eryngii (P group) or C. comatus (C group), respectively. The concentrations of Cd in the fruiting bodies of mushrooms were 1.83–3.06, 1.04–2.28, and 0.67–2.60 mg/kg in T, P, and C groups, respectively. Besides, the removal rates of endosulfan in all treatments exceeded 87%. The best bioremediation effect in T group might be caused by the mutual promotion of these two kinds of mushrooms. The biomass of mushroom, laccase activity, bacterial counts, and Cd content in mushrooms were significantly enhanced, and the dissipation effect of endosulfan was slightly higher in the co-cultivated group than in the individually cultivated groups. In this study, the effect of co-cultivated macro fungi P. eryngii and C. comatus on the remediation of Cd and endosulfan co-contaminated soil was firstly reported, and the results are important for a better understanding of the co-remediation for co-contaminated soil.

Separation, purification and antioxidant activity of polysaccharide from Coprinus comatus

We compared the ways of deproteinization for crude polysaccharides of Coprinus comatus, and finally selected Sevage method as the optimal method. Two main fractions of Ccp-I-A and Ccp-I-B were obtained after DEAE-52 cellulose and Sephadex G-200 chromatography, both were white-floc, soluble in water, insoluble in absolute ethyl alcohol, acetone and other organic solvents. Additionally, Fehling reagent, CTAB, Sulphuric acid-carbazole, I-KI and FeCl₃ reaction were all negative. GC analysis showed Ccp-I-A was composed of mannitose, glucose and galactose in molar ratios of 2.03:9.52:1, whereas Ccp-I-B was composed of fucose and galactose with molar ratios of 1:5.21. Antioxidant activity test showed that Ccp-I-A and Ccp-I-B had good scavenging abilities on DPPH and ·OH. Compared to Ccp-I-B,the scavenging activity of Ccp-I-A was much stronger, and the scavenging rate could reach 72.1% and 55.3% respectively when the concentration was 300 μg/mL.

Antioxidant Components and Antioxidant Activities of Hydroglyceroalcoholic Extract from Fresh Mushroom Mycelium

The in vitro antioxidant activity, as well as the hydroglyceroalcoholic extracts main active components from mycelia of Pleurotus ostreatus and Coprinus comatus, were determined. The mycelium submitted for extraction was obtained by submerged cultivation in a bioreactor. The hydroglyceroalcoholic extracts, in particular, those of P. ostreatus PSI101109 and PQMZ91109 mycelium had a considerable antioxidant activity, and was compared to the overall constituent of flavonoids plus phenolic compounds. According to the extracts HPLC assessment, the key phenolic acid evident from the extracts was gentisic acid. The homogentisic, gallic and protocatechuic acids were also determined. Catechin was identified as well, except for C. comatus M8102 and P. ostreatus PBS281009 mycelia extracts. Since catechol and gentisic acid levels were high, respectively, there was a correlation with the absence of carotenoid compounds in hydroglyceroalcoholic extracts (P. ostreatus PSI101109). There is a correspondence between the hydroglyceroalcoholic extracts� key antioxidant activities with the catechin and polyphenolic acids levels identified in each extract.

BETWEEN THE BIOACTIVE EXTRACTS OF EDIBLE MUSHROOMS AND PHARMACOLOGICALLY IMPORTANT NANOPARTICLES: NEED FOR THE INVESTIGATION OF A SYNERGISTIC COMBINATION - A MINI REVIEW

ABSTRACTThe pharmacological potential of bioactive compounds extracted from mushrooms has been studied to a reasonable level. In the same vein, thebioactivity of nanoparticles has also been investigated and reported to be of potential pharmacological benefit. No doubt, there is a reasonableamount of claims regarding the vast activity of the mushroom extracts and nanoparticles on the tested cell lines and microorganisms. In this paper,a review of the recent application of bioactive compounds extracted from two edible mushrooms (Coprinus comatus and Lactarius deliciosus), aswell as some of the recently reported studies on some nanoparticles of pharmacological potentials, was carried out. In order to check for synergy inthe bioactivity of the mushroom extracts when co-administered with nanoparticles, an investigation on the synergistic application of the materialsthrough the encapsulation of the bioactive extracts from the mushroom onto the nanoparticle was proposed. The supposed synergy in the activity ofthe extract-nanoparticle complex could hold the key to improved activity of nutraceuticals against resistant microorganisms and tumor cells.Keywords: Coprinus comatus, Lactarius deliciosus, Nanoparticles, Synergy, Bioactive extracts.

A comparative overview of antioxidative properties and phenolic profiles of different fungal origins: fruiting bodies and submerged cultures of Coprinus comatus and Coprinellus truncorum

Bioactive properties of fungi considerably differ between the fruiting body (FB) and the submerged culture as regards mycelia (M) and the fermentation broth (F). Antioxidant properties of hot-water extracts obtained from three different fungal origins: FB, M and F of two autochthonous fungal species (Northern Serbia), Coprinus comatus and Coprinellus truncorum were investigated. Free radical scavenging capacity (RSC) was evaluated in vitro by the DPPH assay and reducing power ability (FRAP assay). Considering possible bioactive properties of different compounds present in fungal extracts, the content of total proteins (TP), phenols (TC) and flavonoids (TF) were investigated colorimetrically. The chemical characterisation of the examined extracts was evaluated using the HPLC-MS/MS method. C. comatus showed the strongest RSC activity; more precisely, fermentation broth extract (FCc) on DPPH radicals (IC50=5.06μgmL-1) and fruiting body extract (FBCc) for the FRAP assay (42.86mg ascorbic acid equivalents (AAE)/g). Submerged M extract of both species showed the highest TC (MCc 81.95mg gallic acid eq (GAE)/g d.w.; MCt 81.64mg GAE/g d.w.), while FB extracts contained the highest content of TP. Comparing LC-MS phenolic profiles between species-interspecifically and among different fungal origins-intraspecifically (fruiting bodies and submerged cultures), high variations were noticed. In submerged M or F extracts of C. comatus, vanillic, gallic, gentisic and cinnamic acids were detected, as opposed to FB. Considering that diverse phenolic profiles of detected antioxidant compounds were obtained by submerged cultivation, this type of cultivation is promising for the production of antioxidant substances.

Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp.

During the last decades, intensive application of synthetic fungicides in the agricultural crop protection practice caused growing concern for the existence of toxic chemical residues in food as well as in the whole environment. Instead of using synthetic fungicides, it is suggested that crop protection be carried out by using preparations based on compounds of natural origin (secondary metabolites of plants or microorganisms, including macrofungi from Basidiomycota) as biological control agents. The potential of macrofungal species as biocontrol agents was analyzed in this investigation of eight autochthonous species from different locations in Serbia. Both the terricolous species: Coprinus comatus, Coprinellus truncorum, Amanita strobiliformis, Hydnum repandum and the lignicolous species: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor and Bjerkandera adusta were examined, with an aim to detect some novel sources of antifungal agents. This study surveyed antifungal activity of selected macrofungal extracts (MeOH, EtOH and CHCl3) against phytopathogenic Fusarium and Alternaria strains isolated from garlic, soybean and rice: F. proliferatum, F. verticillioides, F. proliferatum, F. graminearum and A. padwickii. Microdilution method in 96 well microplates was applied for the estimation of antifungal effects of macrofungi extracts in the range from 24.75 to 198.00 mg/ml and determination of minimal inhibitory (MIC) and minimal fungicidal concentration (MFC). EtOH extract of mychorhizal species H. repandum showed antifungal activity against all analyzed phytopathogenic strains, with the strongest effect on Fusarium strains (MIC 24.75 mg/ml; MFC 24.75 mg/ml). Among others, MeOH extracts of S. subtomentosum and C. micaceus showed similar effects while only B. adusta showed slight effect on Fusarium strains (MIC 24.75-99.00 mg/ml; MFC 24.75-99.00 mg/ml) and none effect on A. padwickii. The obtained results indicate the possibility of using examined extracts as efficient antifungal agents and provide the basis for the new formulations of biocontrol agents against phytopathogenic fungi in the future.

Coprinuslactone protects the edible mushroom Coprinus comatus against biofilm infections by blocking both quorum-sensing and MurA.

Pathogens embedded in biofilms are involved in many infections and are very difficult to treat with antibiotics because of higher resistance compared with planktonic cells. Therefore, new approaches for their control are urgently needed. One way to search for biofilm dispersing compounds is to look at defense strategies of organisms exposed to wet environments, which makes them prone to biofilm infections. It is reasonable to assume that mushrooms have developed mechanisms to control biofilms on their sporocarps (fruiting bodies). A preliminary screening for biofilms on sporocarps revealed several species with few or no bacteria ontheir sporocarps. From the edible mushroom Coprinus comatus where no bacteria on the sporocarp could be detected (3R,4S)-2-methylene-3,4-dihydroxypentanoic acid 1,4-lactone, named coprinuslactone, was isolated. Coprinuslactone interfered with quorum-sensing and dispersed biofilms of Pseudomonas aeruginosa, where it also reduced the formation of the pathogenicity factors pyocyanin andrhamnolipid B. Coprinuslactone also damaged Staphylococcus aureus cells in biofilms at subtoxic concentrations. Furthermore, it inhibited UDP-N-acetylglucosamine enolpyruvyl transferase (MurA), essential for bacterial cell wall synthesis. These two modes of action ensure the inhibition of a broad spectrum of pathogens on the fruiting body but may also be useful for future clinical applications.

Investigation and analysis of a mushroom poisoning in Hejin Shanxi Lang Nan, Wu Liming, Li Haijiao, Zhou Jing, Yuan Yuan, Sun Jian, He Qian, Sun Chengye

Objective Analyzing on-site disposal process of a mushroom poisoning in Hejin Shanxi, and providing references for future similar emergency disposal. Methods We collected epidemiological investigation, clinical treatment data and suspicious poisonous mushroom sample. Mushroom sample was identified by molecular analysis. Results Epidemiological investigation found that all patients ate different quantity fried mushroom and the latency was 0.5-1.5 h. Clinical manifestation was nausea, emesis, stomachache, diarrhea, salivation, and weak; in addition 4 people had the clinical symptoms of miosis and blurred vision, and 2 people had abnormal liver function index. Being treated with gastric lavage, protecting liver, diuresis and supporting treatment, all patients cured discharged from hospital after 8 days. The mushroom sample was identified Coprinopsis atramentaria, Coprinus comatus and Inocybe cf rimosa. Conclusions Coprinopsis atramentaria, Coprinus comatus and Inocybe cf rimosa were poisonous mushroom. According to epidemiology, clinical feature and molecular biological identification, the accident can be confirmed a more than one species poisonous mushroom poisoning incident. Key words: Poisonous mushrooms; Poisoning; Molecular biological identification; Medical treatment

Coprinus comatus-derived nitrogen-containing biocarbon electrocatalyst with the addition of self-generating graphene-like support for superior oxygen reduction reaction

The development of nitrogen-rich biomass-derived carbon catalysts provides an attractive perspective to substitute for Pt-based electrocatalysts for oxygen reduction reaction (ORR). We here report a facile strategy for synthesis of a nitrogen-doped biocarbon/graphene-like composite electrocatalyst by pyrolyzing a solid-state mixture of coprinus comatus biomass and melamine under nitrogen protection. The graphtic carbon nitride formed by polycondensation of melamine at 600°C acts as a self-sacrificing template to generate the nitrogen-doped graphene-like sheet, which can function as an inserting agent and self-generating support. The composite catalyst exhibits the most promising catalytic activity towards the four-electron ORR with a half-wave potential of around 0.83V (vs. RHE), and more excellent stability and tolerance to methanol/ethanol compared to the commercial Pt/C catalyst. It is interestingly found that both a higher content of nitrogen and a larger ratio of graphitic-nitrogen species, which may derive from self-addition of graphene-like support into the catalyst, can effectively improve the electrocatalytic activity. The planar N group may be the nitrogen functionality that is most responsible for maintaining the ORR activity in alkaline medium. This study can largely encourage the exploration of high-performance carbon-based catalysts from economical and sustainable fungus biomass.

ANTIMICROBIAL ACTIVITY OF CRUDE ETHANOLIC EXTRACTS FROM SOME MEDICINAL MUSHROOMS

In this paper the antimicrobial activity of 1 year old crude ethanolic extracts obtained from Cordyceps sinesis, Laricifomes officinalis, Oudemansiella mucida and Coprinus comatus were investigated. The antimicrobial activities of extracts against two Gram-positive bacteria (Bacillus thuringiensis, Staphylococcus aureus) and two Gram-negative bacteria (Klebsiella pneumoniae, Enterobacter aerogenes) were determined by disk diffusion and microbroth dilution method according by EUCAST in 96-well microplates. Microorganisms were obtained from Czech Collection of Microorganisms. Absorbance after and before the experiment were substracted, converted to binary system and obtained values to Probit analysis were used. Not all macromycetes ethanolic extracts showed antimicrobial activity against tested bacteria. Antimicrobial activity determined by MIC methodology showed extracts from Oudemansiella mucida, Cordyceps sinesis, Coprinus comatus in the tested range. Conversely, the best antimicrobial activity tested by disc diffusion methods showed extract from Laricifomes officinalis. Equally, more better studying of antimicrobial activity in these mushrooms will needed.

A mushroom-derived amino acid, ergothioneine, is a potential inhibitor of inflammation-related DNA halogenation.

Myeloperoxidase (MPO)-generated halogenating molecules, such as hypochlorous acid and hypobromous acid (HOBr), in inflammatory regions are postulated to contribute to disease progression. In this study, we showed that ergothioneine (EGT), derived from an edible mushroom, inhibited MPO activity as well as the formation of 8-bromo-2'-deoxyguanosine in vitro. The HOBr scavenging effect of EGT is higher than those of ascorbic acid and glutathione. We initially observed that the administration of Coprinus comatus, an edible mushroom containing a high amount of EGT, inhibited the UV-B-induced inflammatory responses and DNA halogenation, suggesting that EGT is a promising anti-inflammatory agent from mushrooms.

Cytotoxic, antimigratory, pro-and antioxidative activities of extracts from medicinal mushrooms on colon cancer cell lines

Methanol extracts of five commercially available mushroom species (Phellinus linteus (Berk. et Curt) Teng, Cordyceps sinensis (Berk.) Sacc., Lentinus edodes (Berk.) Pegler, Coprinus comatus (O. F. M?ll.) Pers. and Ganoderma lucidum (Curtis) P. Karst), traditionally used as anticancer agents, were evaluated in vitro for their total phenol and flavonoid contents, cytotoxic and antimigratory activities and antioxidant/prooxidant effects on colon cancer cell lines (HCT-116 and SW-480). Spectrophotometric methods were used for the determination of total phenol content, flavonoid concentrations and DPPH activity of the extracts. Cytotoxic activity was measured by the MTT assay. The antimigratory activity of extracts was determined using the Transwell assay and immunofluorescence staining of ?-catenin. The prooxidant/antioxidant status was followed by measuring the superoxide anion radical (O2?-), nitrite and reduced glutathione (GSH) concentrations. Our results show that the highest phenolic and flavonoid content was found in P. linteus, and its DPPH-scavenging capacity was significantly higher than in other samples. The P. linteus extract significantly decreased cell viability of both tested cancer cell lines. All other extracts selectively inhibited SW-480 cell viability, but did not show significant cytotoxic activity. The mushroom extracts caused changes in the prooxidant/antioxidant status of cells, inducing oxidative stress. All extracts tested on HCT-116 cells demonstrated significant antimigratory effects, which correlated with increased production of O2?- and a reduced level of ?-catenin protein expression, while only P. linteus showed the same effect on SW-480 cells. The results of the present research indicate that the mushroom extracts causes oxidative stress which has a pronounced impact on the migratory status of colon cancer cell lines.

Mercury bio-extraction by fungus Coprinus comatus: a possible bioindicator and mycoremediator of polluted soils?

The Shaggy Ink Cap (Coprinus comatus), which is a common in wild in northern hemisphere was examined in field for potential to be used as possible bio-extractor of Hg from polluted grounds but also as possible bioindicator of urban soils (roadside, barren lands, lawns) pollution with Hg. The contents of Hg in caps and stipes of C. comatus from the grounds examined in this study correlated positively with the levels of soil contamination. Analysis of sets of data available worldwide on Hg in C. comatus and soils beneath-fruiting bodies showed on a positive correlation between degree of soil and mushroom contamination. Hence, C. comatus could be considered as a sensitive species and with bioindication and bioremediation potency for soils polluted with Hg in further studies. Young-fruiting bodies of C. comatus are edible and considered excellent if consumed soon after pick-up. Eating them when foraged from the urban places can provide to a consumer Hg at relatively high dose, while unresolved question is absorption rate of Hg compounds contained in ingested mushroom meal.

Controle alternativo sobre Meloidogyne incognita em soja

The alternative control is an environmentally safe and ecologically viable option for controlling plant pathogens. Considering the great importance of nematodes in soybean, this study aimed to evaluate the efficacy of nematophagous fungi against the root-knot nematode Meloidogyne incognita in soybean. The experimental design was in randomized blocks, with five treatments and four replicates. The treatments were Pochonia chlamydosporia , Paecilomyces lilacinus , Coprinus comatus , a mixture of P. chlamydosporia and P. lilacinus , and control plants inoculated with M. incognita and without treatment. We evaluated number of eggs and second stage juveniles (J2) in soil and roots, number of galls and reproduction factor (RF). There were no differences among the treatments and the control plants for number of eggs and J2 in soil and roots, number of galls and RF. The P. chlamydosporia treatments caused the reduction of the number of J2 in roots. Thus, was possible to conclude that P. chlamydosporia has potential to control M. incognita in soybean plants. Normal 0 21 false false false PT-BR X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:Tabela normal; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri,sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;}