Abstract
In this paper the antimicrobial activity of 1 year old crude ethanolic extracts obtained from Cordyceps sinesis, Laricifomes officinalis, Oudemansiella mucida and Coprinus comatus were investigated. The antimicrobial activities of extracts against two Gram-positive bacteria (Bacillus thuringiensis, Staphylococcus aureus) and two Gram-negative bacteria (Klebsiella pneumoniae, Enterobacter aerogenes) were determined by disk diffusion and microbroth dilution method according by EUCAST in 96-well microplates. Microorganisms were obtained from Czech Collection of Microorganisms. Absorbance after and before the experiment were substracted, converted to binary system and obtained values to Probit analysis were used. Not all macromycetes ethanolic extracts showed antimicrobial activity against tested bacteria. Antimicrobial activity determined by MIC methodology showed extracts from Oudemansiella mucida, Cordyceps sinesis, Coprinus comatus in the tested range. Conversely, the best antimicrobial activity tested by disc diffusion methods showed extract from Laricifomes officinalis. Equally, more better studying of antimicrobial activity in these mushrooms will needed.
Highlights
Nature is a very good source of many medical compounds for thousands of years
The present work is focus to antimicrobial activity of 1 year old medicinal mushrooms extracts isolated from Cordyceps sinesis, Laricifomes officinalis, Oudemansiella mucida and Coprinus comatus against some selected Grampositive and Gram-negative bacteria
In the other side authors like Demir and Yamac, 2008 tested Coprinus comatus basidiocarp extract dissolved in different solutions and they determined its antimicrobial activity against Staphylococcus aureus, Enterococcus faecium, Proteus vulgaris and Candida glabrata
Summary
Nature is a very good source of many medical compounds for thousands of years. In the last decades problem with antibiotic resistant bacteria has emerged. There is many different studies about antimicrobial activity of different types of fungal extracts from India (Seena et al, 2003; Quereshi et al, 2010 ) and China (Gao et al, 2005). In this country fungi medicine has tradition for many years ago. Only compounds from microscopic fungi are on the market as antibiotics till (Lindequist et al, 2005). The present work is focus to antimicrobial activity of 1 year old medicinal mushrooms extracts isolated from Cordyceps sinesis, Laricifomes officinalis, Oudemansiella mucida and Coprinus comatus against some selected Grampositive and Gram-negative bacteria
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