Conventional Packing Research Articles

Introduction of Curvature in Amphipathic Oligothiophenes for Defined Aggregate Formation

In this study the possibility to control the size and shape of self-assembled structures through the local curvature of their molecular building blocks has been investigated. To this end a series of amphipathic conjugated oligothiophenes with a well-defined curvature of their backbone has been designed and synthesized. The molecular (local) curvature of these oligothiophenes resulted from a preference for cis instead of trans conformations at specific positions along the oligothiophene backbone, which can be controlled by the sequence of hydrophilic and hydrophobic groups, while their ratio was kept constant. The self-assembly of ter-, sexi-, and dodecathiophenes appeared to be a low-cooperative process, involving the formation of premicellar aggregates at sub-millimolar concentrations, which at concentrations in the millimolar regime transformed into micelles and cylindrical micelles. The aggregates display fine structures with dimensions reminiscent of the thiophene molecules. The structure-morphology relationship of the ter- and sexithiophenes could be described by conventional packing theory. However, with the dodecathiophene, the backbone curvature governed the formation of cylindrical aggregates with a well-defined diameter. These results demonstrate that it is possible to control the aggregation morphology of simple amphipathic oligothiophenes by implementation of an additional structural motif namely, the curvature.

The need for intranasal packing in endoscopic endonasal surgery

Conclusion: The present findings indicate that conventional nasal packing is not required for endoscopic endonasal surgery. Objectives: To evaluate the routine use of packing in endoscopic endonasal surgery. Methods: From January 2006 through January 2009, 146 consecutive patients who underwent endoscopic endonasal surgery performed by the same surgeon in Kyoto University Hospital were evaluated. The surgical procedure was ended with conventional gauze packing in 70 consecutive patients from January 2006 through August 2007 (Packing group), and placing of oxidized cellulose on operative sites was performed in 76 consecutive patients from September 2007 to January 2009 (Non-packing group). We analyzed demographic characteristics, comorbidities, surgical procedures, incidence of packing the nose after excessive postoperative bleeding, and occurrence of postoperative adhesion. Results: No significant differences in the demographic characteristics, except for gender, and in comorbidities were found between the two groups. The number of endoscopic sinus surgery procedures with septoplasty and/or turbinoplasty, or tumor extirpation in the Non-packing group was significantly larger than that in the Packing group. There was no significant difference in the incidence of postoperative bleeding or postoperative adhesion between the two groups.

Intensification of G/ L absorption in microstructured falling film. Application to the treatment of chlorinated VOC’s – part I: Comparison between structured and microstructured packings in absorption devices

The absorption of tetrachloroethylene – the VOC – in di-ethyl-hexyl-adipate – the solvent – was carried out as an example of gaseous waste treatment. Two gas–liquid contactors were used: a column provided with as structured Sulzer EX ® packing and a microstructured falling-film absorber provided with thin vertical channels, manufactured by the Institut für Mikrotechnik Mainz (IMM). The overall transfer coefficient of VOC, K G a, was calculated from the absorption efficiency of the various runs carried out, allowing comparison of the two gas–liquid contactors. Due to the high solubility of the considered VOC, mass transfer was shown to be mainly controlled by gas-side transfer rates. Transfer coefficient K G a of the two absorbers were found to be comparable, but with gas and liquid velocity in the microstructured absorber from one to two orders of magnitude below those in the column, expressing the high transfer performance offered by the microsystem. Moreover, the thickness of the liquid film in the channels was below 100 μm, much lower than that in a structured packing near 500 μm. This shows that lower liquid flow rates can be used for efficient absorption in the microsystem. It is shown that contrary to conventional structured packing, the designed contact specific area in the microabsorber strictly corresponds to the interfacial G/ L surface. This enables more compact and to miniaturize G/ L contactors to be designed.

External auditory canal stenting utilizing a useful rolled, tapered silastic sheet (RTSS) post middle ear surgery

Objective To overcome the demerits of conventional postoperative aural packing, we developed a useful protocol for postoperative stenting of the external auditory canal after middle ear surgery which enables transcanal drainage and simultaneously allows for visual inspection and treatment of the canal, as necessary. Methods Twenty-four surgeries, 21 patients underwent tympanoplasty with a postaural incision. At the end of all surgical procedures, the external auditory canal was packed with a 0.3 mm thickness rolled, tapered silastic sheet (RTSS) with antibiotic ointment applied to one surface. The inserted RTSSs were removed at 5–10 days postoperatively. We assessed the efficacy and the reliability of the RTSS. Results In 23 ear surgeries on 20 patients, we achieved successful postoperative ear packing utilizing our RTSS. With these patients, the tympanic membrane and the external auditory meatus were able to be observed immediately after the completion of the stenting during the surgery and the removal of the gauze over the operated ear at Day 1 to Day 3 postoperatively. During 4 surgeries with ventilation tube insertion to the tympanic membrane, there was secretion through the inserted ventilation tube which was easy to suction. In one surgery, on one patient, additional packing materials were utilized once only during a sandwich graft myringoplasty. No patients showed any harmful effects during the postoperative period. Conclusions The useful and reliable RTSS, with antibiotic ointment applied to one surface, has several positive advantages that the conventional packing methods do not.

A Limited Feedback Joint Precoding for Amplify-and-Forward Relaying

This paper deals with the practical precoding design for a dual hop downlink with multiple-input multiple-output (MIMO) amplify-and-forward relaying. First, assuming that full channel state information (CSI) of the two hop channels is available, a suboptimal dual hop joint precoding scheme, i.e., precoding at both the base station and relay station, is investigated. Based on its structure, a scheme of limited feedback joint precoding using joint codebooks is then proposed, which uses a distributed codeword selection to concurrently choose two joint precoders such that the feedback delay is considerably decreased. Finally, the joint codebook design for the limited feedback joint precoding system is analyzed, and results reveal that independent codebook designs at the base station and relay station using the conventional Grassmannian subspace packing method is able to guarantee that the overall performance of the dual hop joint precoding scheme improves with the size of each of the two codebooks. Simulation results show that the proposed dual hop joint precoding system using distributed codeword selection scheme exhibits a rate or BER performance close to the one using the optimal centralized codeword selection scheme, while having lower computational complexity and shorter feedback delay.

A NEW TECHNIQUE FOR DURAL SUTURING WITH FASCIA GRAFT FOR CEREBROSPINAL FLUID LEAKAGE IN TRANSSPHENOIDAL SURGERY

One of the most common postoperative complications of surgery using a transsphenoidal approach is cerebrospinal fluid (CSF) leakage, which typically results from inadequate repair of a CSF fistula created at the time of the initial operation. Most techniques use autologous tissue grafts of fat, muscle, or fascia lata, with or without the use of postoperative lumbar CSF drainage; however, patients demonstrate a relatively high incidence of CSF rhinorrhea, especially after extended procedures. We have developed a new technique of dural suturing with fascia graft using special suture-tying microinstruments. Twenty-one consecutive patients with suprasellar tumors underwent dural suturing with fascia graft via new suture-tying microinstruments between January 2004 and December 2007. The 21 patients were retrospectively divided into 2 groups according to the transsphenoidal technique used. Group 1 consisted of 16 patients whose large dural defects were closed with a fascia graft suture for CSF leakage during or after an extended transsphenoidal approach. Group 2 consisted of 5 patients whose dural defects were closed with a fascia graft suture for postoperative CSF rhinorrhea after a conventional transsphenoidal approach. None of the 21 patients developed any clinical symptoms of CSF leakage. There were no complications or infections. For 8 patients in group 1 and the 5 patients in Group 2, no postoperative lumbar drainage was performed after dural suturing with fascia graft, and none of the 13 patients developed postoperative CSF rhinorrhea. Our dural suturing technique with fascia graft may be more reliable than the conventional packing technique in achieving watertight dural closure and for the prevention of postoperative CSF rhinorrhea. Watertight dural suturing with fascia graft and the leaking point suture could allow surgeons to avoid unnecessary postoperative lumbar drainage.

Use of nasal packs and intranasal septal splints following septoplasty

The aim of this study was to compare the efficacy of a trans-septum suturing technique with conventional nasal packing and intranasal splints in the classic septoplasty operation. The study is a prospective, randomized clinical trial. 114 patients underwent septoplasty for septal deviation and ensuing nasal obstruction. These patients were divided into two groups: packing (using intranasal septal splints and antibiotic meshes at the end of the operation) and non-packing (using four separate trans-septum through and through horizontal mattress sutures without any mesh or intranasal splint insertion). Randomization was performed using the four block randomization system. Patients who failed the regular follow-up were excluded, and the two groups were compared for postoperative bleeding, hematoma, perforation and synechiae. Patients were asked to record pain levels using a visual analogue scale. The authors found no significant statistical differences between the two groups in the parameters studied, but significantly higher pain levels were noted in the patients in the packing group. The final results confirmed that patients who underwent septoplasty, intranasal packing and septal splint insertion did not benefit more than those who had trans-septum through and through suturing.

Nasentamponade nach FESS – ein Auslaufmodell?

National and international tendencies to avoid nasal packing after FESS are closed related to the surgical surgery (atraumatic endoscopic surgery, avoidance of resection of turbinates, meticulose coagulation). At the end of each operation a benefit-risk-analysis should be performed, whether nasal packing is indicated and which one. In endonasal surgery only those conventional nasal packing materials should be used - if necessary - which have a smooth surface and minimize mucosal damage, potential worsening of wound healing and negative impact on patient comfort. So called hemostatic/resorbable materials are a first step to these direction. But they are critical, because these materials cause increased synechiae and sometimes foreign body reactions because of incorporation into the mucosa. Occlusion of the nose via simple taping of the nasal entrance is a simple and very effective method to create a moist environment to optimize endonasal wound healing or management of a dry nose.

Packing density measurement and modelling of fine aggregate and mortar

A newly developed wet packing method has been applied to measure the packing densities of blended fine aggregates (each a mixture of fine aggregates of different sizes) and mortars (each a mixture of cement and blended fine aggregate) under the wet condition, with or without superplasticizer added, and with or without compaction applied. For the blended fine aggregates, the conventional dry packing method has also been employed to measure their packing densities under the dry condition and the results show that the packing density of fine aggregate is generally higher under wet condition than under dry condition. For both the blended fine aggregates and mortars, the measured packing densities have been compared to the predicted packing densities by two existing packing models. Good agreement between the measured and predicted packing densities has been achieved with the mean absolute error being 2.1% for the blended aggregates and 1.1% for the mortars. This is the first time that the packing densities of mortar samples are directly measured and compared to predictions by packing models to verify the applicability of the wet packing method and the packing models.

Synthesis of biomorphic zeolite honeycomb monoliths with 16 000 cells per square inch

Zeolite NaX and silicalite honeycomb monoliths were synthesized via hydrothermal growth of zeolites on the interior surface of cuttlebone by a novel flow coating technique. The resultant samples were characterized by SEM, XRD, EDX, N2 adsorption and TGA. The loading of nano-crystalline silicalite (36.4 wt%) was higher than that obtained for NaX type zeolite. The biomorphic honeycomb monoliths were found to have an exceptionally high cell density of 16 000 cells per square inch (cpsi) which is ten times higher than the best synthetic ones. The honeycombs were evaluated for their hydrodynamic and kinetic properties, showing substantial improvement of mass transfer rate with lower pressure drop than conventional zeolite packing.

Effect of the flask contention method on the displacement of maxillary denture teeth

Keeping the flask closed may reduce the stress released by the resin dough after the pressing step, preserving the tooth relationship pattern. The aim of this study was to analyze the influence of the flask contention method on tooth displacement in acrylic resin complete dentures. Twenty identical maxillary complete dentures were made using the heat-cured acrylic resins Classico and QC-20, polymerized by long and fast polymerization cycles respectively, and randomly assigned to 4 test groups according to the conventional packing method and RS tension system. Transversal and anteroposterior distances between specific teeth were measured with a linear optical microscope before and after denture processing. Analysis of variance (ANOVA) and Tukey test were used to compare the groups (p<.05). Results showed that tooth movement was significantly greater for the distance RI-LI in comparison with the others, and the tooth movement was not significantly influenced by the packing method. Within the limitations of this study, the RS tension system presented a similar performance in reducing the tooth movement, when compared with the conventional packing method. The anterior region of the denture may present changes in the tooth position after processing, which need to be clinically adjusted.

CALCULATION PROCEDURE FOR FLOODING IN PACKED COLUMNS USING A CHANNEL MODEL

The combined effect of a channel-based approach for dry pressure drop and the Buchanan equation for wet pressure drop in packed beds has been numerically evaluated within the flooding region. The flooding point is an important design parameter since it establishes the maximum hydrodynamic capacity at which a packed column can operate. Upon analyzing the aforementioned approach, it was found that the usual practice of fixing a “reasonable” wet pressure drop at the flooding point (e.g., 1025 Pa/m) may not yield the correct flooding velocity of the gas, particularly at higher liquid loads. In fact, numerical evaluations of the aforementioned model showed a rather “retrograde” non-monotonic behavior of pressure drop with respect to the f factor of the gas near flooding at different liquid loads. A calculation procedure was therefore devised in this work to correctly compute the flooding point for a given liquid load when using the aforementioned modeling approach. Interestingly, it was found that the correct flooding velocity can be directly computed from liquid holdup below the gas loading point. To illustrate the use of the procedure, maximum capacity calculations were performed for a well-known random packing, a conventional structured packing, and a novel catalytic structured packing.

Molecular shape recognition-structure correlation in a phenylalanine-based polymer–silica composite by surface-initiated atom transfer radical polymerization

A polymerizable l-phenylalanine-derived monomer (N′-octadecyl-Nα[4-(acryloyloxy)-butanoyl]-l-phenylalanineamide (3)) has been newly synthesized and characterized. We reported an advanced approach to molecular shape recognitive hybrid materials that involves immobilization of radical initiator on mesoporous silica particles (average diameter, pore size and surface area are 4μm, 12nm, and 300m2g−1, respectively) and surface-initiated atom transfer radical polymerization of monomer 3 from initiator-grafted particles. All samples were characterized by elemental analysis, thermogravimetric analysis, diffuse reflectance infrared Fourier transform spectroscopy, solid state NMR measurements (13C CP/MAS NMR and 29Si CP/MAS NMR), differential scanning calorimetry and scanning electron microscopy. The obtained polymer–silica hybrid material was used as a stationary phase for reversed-phase high performance liquid chromatography (RP-HPLC) to investigate its molecular shape recognition ability towards polycyclic aromatic hydrocarbons (PAHs). The new composite material showed better planarity selectivity than octadecylsilane stationary phase as a conventional RP-HPLC packing material and also enhanced linearity selectivity compared with the recently reported poly(N′-octadecyl-Nα-(4-vinyl)-benzoyl-l-phenylalanineamide)-grafted silica. Such selectivity enhancement can be attributed to the combination of hydrophobic effect due to octadecyl chains and multiply carbonyl π–benzene π interaction between the amide groups of the stationary phase and delocalized electrons of PAHs.

Simultaneous Biofiltration of H2S, NH3and Toluene using an Inorganic/Polymeric Composite Carrier

Simultaneous removal of ternary gases of NH3, H2S and toluene in a contaminated air stream was investigated over 180 days in a biofilter. A commercially available inorganic/polymeric composite chip with a large void volume (bed porosity > 0.80) was used as a microbial support. Multiple microorganisms including Nitrosomonas and Nitrobactor for nitrogen removal, Thiobacillus thioparus (ATCC 23645) for H2S removal and Pseudomonas aeruginosa (ATCC 15692), Pseudomonas putida (ATCC 17484) and Pseudomonas putida (ATCC 23973) for toluene removal were used simultaneously. The empty bed residence time (EBRT) ranged from 60 - 120 seconds and the inlet feed concentration was 0.0325 g/m 3 - 0.0651 g/m 3 for NH3, 0.0636 g/m 3 - 0.141 g/m 3 for H2S, and 0.0918 g/m 3 - 0.383 g/m 3 for toluene, respectively. The observed removal efficiency was 2% 98% for NH3, 2% - 100% for H2S, and 2% - 80% for toluene, respectively. Maximum elimination capacity was about 2.7 g/m 3 /hr for NH3, > 6.4 g/m 3 /hr for H2S and 4.0 g/m 3 /hr for toluene, respectively. The inorganic/polymeric composite carrier required 40 - 80 days of wetting time for biofilm formation due to the hydrophobic nature of the carrier. Once the surface of the carrier was completely wetted, the microbial activity became stable. During the long-term operation, pressure drop was negligible because the void volume of the carrier was two times higher than the conventional packing materials.

Synthesis, Self‐Assembling Properties, and Atom Transfer Radical Polymerization of an Alkylated L‐Phenylalanine‐Derived Monomeric Organogel from Silica: A New Approach To Prepare Packing Materials for High‐Performance Liquid Chromatography

The monomer N'-octadecyl-N(alpha)-(4-vinyl)-benzoyl-L-phenylalanineamide (4) based on L-phenylalanine has been simply but effectively synthesized, and its self-assembling properties have been investigated. FTIR and a variable-temperature (1)H NMR spectroscopic investigation demonstrated that the aggregation of compound 4 in various organic solvents is due to the formation of intermolecular hydrogen bonds among the amide moieties. UV/Vis measurements indicated that the multiple pi-pi interactions of the phenyl groups also contribute to the self-assembly. As was observed by (13)C cross-polarization magic-angle spinning (CP/MAS) NMR and variable-temperature (1)H NMR measurements, the ordered alkyl chains also played an important role in the molecular aggregation by van der Waals interactions. Compound 4 was polymerized by surface-initiated atom transfer radical polymerization from porous silica gel to prepare a packing material for HPLC. The results of thermogravimetric analysis showed that a relatively large amount of polymer was grafted onto the silica surface. The organic phase on silica was in a noncrystalline solid form in which the long alkyl chain exists in a less-ordered gauche conformation. Analysis of chromatographic performance for polyaromatic hydrocarbon samples showed higher selectivity than conventional reversed-phase HPLC packing materials.

Separation of Alcohol−Water Solutions by Distillation through Hollow Fibers

New results of an investigation on distillation in hollow fiber structured packing are discussed. The membranes used here for separation of methanol−, ethanol−, and 2-propanol−water solutions are nonselective coated hollow fibers, unlike those of membrane distillation. The fibers are nonselective with coating and have little resistance to mass transfer. Because liquid flows inside the lumens of the fibers and vapors flow countercurrently outside the fibers, this distillation process can avoid flooding effectively even at very high flows. As a result, when a mole fraction of alcohol equal to 0.04 was prepared as feed in all distillations, an attractive low value of the height of mass transfer unit (HTU) less than 0.1 m could be obtained at total reflux. Both the distillate concentration and the height of mass transfer unit more significantly decreased with heat rate in alcohols at low molecular weight than in those at high molecular weight. Compared with conventional structured packing, the ordinate data o...

Transarterial Embolization for Intractable Oronasal Hemorrhage Associated With Craniofacial Trauma: Evaluation of Prognostic Factors

This study analyzed the outcomes for clinical application of transarterial embolization (TAE) to treat intractable posttraumatic oronasal hemorrhage in patients who suffered from craniofacial injuries. The charts and radiologic and operative records of 34 patients from January 2002 to December 2007 were retrospectively reviewed. Inclusion criteria focused on the patients whose intractable posttraumatic oronasal hemorrhage associated with craniofacial trauma required TAE treatment. The patients' survival was correlated with prognostic factors with Spearman's rank correlation coefficients. Wilcoxon signed ranks test was used to analyze the differences between the severity of shock before and after TAE, and Fisher's exact test was used to analyze unvaried factors. TAE successfully stopped the posttraumatic oronasal hemorrhage in 27 of 34 patients (79.4%). The internal maxillary artery was the most common hemorrhaging vessel requiring embolization. Successful hemostasis by TAE significantly contributed to patient survival (p = 0.001). In addition, higher Glasgow Coma Scale score (>/=8) at presentation, lower shock index (calculated as heart rate/systolic blood pressure; </=1.1 and 0.8, before and after TAE, respectively), and injury severity score </=32 positively contributed to the patients' higher survival rate (p < 0.05). During the acute treatment of posttraumatic oronasal hemorrhage, need for craniotomy was not correlated with patient survival, but need for laparotomy to treat the second abdominal hemorrhagic source decreased the rate of patient survival (p = 0.023). TAE may stop intractable posttraumatic oronasal hemorrhage when conventional packing fails to achieve hemostasis. Glasgow Coma Scale score at presentation, shock index before and after TAE, injury severity score, and need for emergent laparotomy can be used to predict the patient prognosis.

One-Trip Completion System Eliminates Perforations

This article, written by Assistant Technology Editor Karen Bybee, contains highlights of paper SPE 101086, "One-Trip Sand-Control/Liner-Hanger/ Big-Bore Completion System," by C.R. Hoffman, SPE, Baker Oil Tools, prepared for the 2006 SPE Asia Pacific Oil &amp; Gas Conference and Exhibition, Adelaide, Australia, 11–13 September. A new completion system replaces conventional tubing-conveyed perforating (TCP) and gravel packing. This technology also eliminates formation damage caused by perforating and reduces skin values. It is a new-generation, screenless-sand-control completion system. A telescoping conduit containing sand-control media composed of stainless-steel beads was chosen for its durability and erosion and plugging resistance. The system contains no explosives, eliminating many issues. Introduction A joint-investment project team consisting of three major operators, a service company, and an independent tool manufacturer was formed and charged with the task of developing a system that eliminated formation damage and greatly reduced skin values. Conventional perforating is a major cause of formation damage, so the primary goal was to eliminate perforations and still achieve connectivity with virgin reservoirs and maintain full-bore production and/or injection rates. Also, one-trip capability and user-friendliness were of primary concern; if this system were to cost operators more throughout the completion of a given well, it would have no merit in the marketplace. Therefore, the system was designed around conventional liner and cementing techniques. Purpose Fractured and compacted zones, perforation debris, and formation blockages are common types of damage that can occur in a conventional-perforation tunnel. Case histories have proved that skin values are significantly increased over the life of a well as a result of perforation-tunnel depth and fines that accumulate or are never properly cleaned from these tunnels. Studies have shown that for a 7½-in.-deep perforation tunnel, there may be as much as 60 lbm/ft of debris that must be cleaned from the wellbore. By design, this new technology addresses these factors affecting well productivity. The new technology had to satisfy the following criteria: one-trip deployment, primary cementing of casing tubulars, connectivity to a virgin reservoir, and sand control, all while maintaining cost effectiveness. One-trip installations reduce rig costs greatly. This new technology also addresses some of the problems associated with conventional cased-hole gravel packs such as incomplete perforation packing, voids in the annular pack, and poor communication with the reservoir. Drilling the New Well Use of a calcium-carbonate-based drill-in fluid while drilling the production or injection interval is quite common today. A manganese weighting agent may be used in these fluids to increase fluid density. The carbonate-based fluids provide a very thin, very uniform filter cake on the wellbore wall, providing stability to sensitive and unconsolidated formations. Also, the carbonate particles are acid soluble. This is imperative for this new system because when the telescoping conduits extend and contact the wellbore, a small amount of this material may be trapped on the face. With a barite-based mud there would be no way to remove this obstruction because barite is inert.

Structure and phase behavior of a disk-necklace polymer: Cyclolinear polymethylsiloxane

The structure and phase transitions of cyclolinear polyorganosiloxane copolymer containing 12-member polysiloxane rings have been studied using synchrotron WAXS, DSC, TEM, variable-temperature AFM and polarized optical microscopy. The primary structure of this polymer can be viewed as a necklace of disk-shaped entities (cyclic groups) connected via flexible linkers. In the mesomorphic state, the presence of two different LC phases has been derived from the analysis of WAXS fiber diffractograms. The morphology of one of the phases shows a conventional hexagonal packing of LC chains where the chain axes are perpendicular to the plane of the 2D hexagonal lattice. The other one, so-called R-phase, has a vertically oriented rectangular 2D lattice formed by inter-chain correlations between the bulky polysiloxane cycles (disks). To our knowledge, such a disk-necklace mesophase in which the LC lattice is parallel to the backbone direction has not been reported in the literature so far.

Ultra-fast separation of wheat glutenin subunits by reversed-phase HPLC using a superficially porous silica-based column

A relatively new, unique column packing material for reversed-phase high-performance liquid chromatography (RP-HPLC) was evaluated for rapid separation of wheat glutenin protein subunits. The product named “Poroshell” by the manufacturer consists of a solid core and a porous coat instead of solid silica spheres used in conventional RP-HPLC column packing. This architecture favours rapid mass transfer, facilitating faster reversed-phase separations of biomolecules compared to conventional silica columns. The main objective of this study was to evaluate the quality of separations of glutenin subunits (GS), as well as to optimize conditions to produce the fastest possible run times without sacrificing resolution using a Poroshell 300SB-C 8 2.1×75 mm column. The stability of GS separations over time was also assessed. Two different bread wheat genotypes were used for optimization of separation conditions and six more common and durum wheat genotypes possessing different subunit combinations were used for further evaluation. Glutenin protein was extracted with 0.08 M Tris–HCl buffer (pH 7.5) containing 50% 1-propanol under reducing conditions after pre-extraction of soluble proteins with 50% 1-propanol. Optimization of GS resolution and sample throughput by RP-HPLC was assessed in response to variation in eluent flow rate, acetonitrile (ACN) gradient, and column temperature. The best resolution of both HMW- and LMW-GS was obtained in 13 min using a 23–44% ACN gradient with a flow rate of 0.7 mL/min at 65 °C. Subunit elution times and integrated areas were highly repeatable even after several hundred injections. Highly satisfactory separation of HMW-GS and quantification of ratio of HMW- to LMW-GS were achieved in less than 4 min per sample using a modified HPLC gradient. Ratio of HMW- to LMW-GS was unaffected by the speed of the separations. As well, the elution order of HMW- and LMW-GS was unaffected by the rapid analysis, compared to conventional RP-HPLC separations, so no new learning was required for interpreting chromatograms and classification of subunits. The rapid RP-HPLC method using the Poroshell column appears to be very well suited for routine quantification of HMW-GS and LMW-GS especially for purposes of wheat quality screening and wheat cultivar development activities where large numbers of samples are typically encountered.