Conventional Activated Sludge Treatment Research Articles

The water energy food nexus: A multi-objective optimization tool

Many methods and tools are used to model and optimize various aspects of the water energy food nexus (WEFN). This work presents a novel software tool for the multi-objective optimization of the WEFN (MOO-WEFN). The model underlying the tool captures all three nexus sectors and the interlinkages between them. In addition, the model imposes constraints that mimic forces affecting the nexus, such as economics, environment, health, and nutrition. The software tool couples Excel with Python, which facilitates the tool's accessibility and ease of use due to its widespread usage and availability. A case study application demonstrates the tool's execution and provides examples of how changes can be made to the data to simulate different nexus developments. Five scenarios are applied to reflect such changes with results demonstrating the overall impact on resource production and consumption. Removing one of the less water-intensive food items results in a 20 % increase in water consumption. Limiting the availability of surface water results in a reallocation of water resources such that 80 % of water needs are satisfied from surface water and groundwater and 20 % from wastewater, compared to the baseline of 100 % surface water. Moreover, upon introduction of the conventional activated sludge treatment technology for wastewater (compared to the incumbent membrane bioreactor technology), energy consumption decreased by 10 %. These results show changes beyond the immediate sector of change and reflect the necessity for integrated modeling that captures the impact of one change on the nexus as a whole, in terms of resource consumption and allocation. Thus, the tool supports the capability of testing how new resources, technologies, and limitations will impact nexus interactions. Furthermore, the tool facilitates carrying out sensitivity and Pareto analyses for data generation and result evaluation.

Life Cycle Assessment Methodology Applied to a Wastewater Treatment Plant

Wastewater treatment plants are highly energy-intensive systems. This research uses Life Cycle Assessment (LCA) to determine the impacts generated during the operation of a wastewater treatment plant. Three different scenarios are analyzed: a baseline scenario that considers a conventional activated sludge treatment technology exploiting data from an existing plant located in central Italy, a second scenario that involves the implementation of MBR technology applied to the baseline scenario, and finally a third scenario that consists of the addition of an anaerobic digester that allows energy recovery from biogas production, followed by a photovoltaic plant capable of supplying the plant energy demand. Global warming potential, eutrophication, and acidification are the environmental categories considered most relevant to emissions. The results showed that the effluent had the highest impact in terms of CO2 equivalent in all three situations due to the presence of N2O. Since emissions from biological processes, transportation, and wastewater are almost similar in all three scenarios, it is preferable to focus on the environmental impacts associated with energy consumption. The third scenario involves careful resource management and the use of treatment technologies that allow for a reduction in the use of nonrenewable energy sources in favor of renewable ones.

Linking conventional activated sludge treatment plant performances for micropollutants removal to environmental risk and SimpleTreat model assessment

The attention to the contaminants of emerging concerns or micropollutants is continuously rising, also stimulated by the new initiatives at European level. The present study focuses on the removal of target micropollutants in a conventional activated sludge wastewater treatment plant which is the most common system and considered a source of micropollutants to the environment. The aims were to evaluate the removal efficiency of different stages of the wastewater treatment plant and to conduct the Environmental Risk Assessment on the final effluent. As indicated by the European’s guidelines on Environmental Risk Assessment, the SimpleTreat4.1 model was applied to this purpose: different values of the biodegradation constant, which represents the key factor for modelling, were applied, and the effects on the quality of prediction were evaluated. The evaluations were carried out thorough 3-year monitoring activity in a full-scale wastewater treatment plant on 10 micropollutants belonging to the classes of illicit drugs, pharmaceuticals and psychoactive. The model was found statistically not satisfactory for most of the investigated micropollutants. A better prediction was obtained when the biodegradation constant was calculated based on the full-scale data. Nonetheless, the results obtained in terms of risk were considered acceptable and comparable among each other.Graphical abstract

Impact of Doxycycline Addition on Activated Sludge Microflora and Microbial Communities

Municipal wastewater treatment plants (WWTPs) are exposed to high concentrations of micropollutants that can impact conventional activated sludge treatment. The consequences of this include failure to meet discharge standards and the disintegration of flocs, leading to poor sludge settleability. This lab-scale study focuses on the influence of doxycycline, an antibiotic widely used against human and animal diseases, on protozoa, metazoa, and bacterial communities under sludge growing conditions. Doxycycline was added to the mixed liquor of a communal WWTP up to 0, 100, 200, and 400 mg of doxycycline L−1 and incubated in batch conditions for 23 days. The regular addition of nutrient and carbon sources was preformed every 2 days to prevent sludge starvation. Sludge growth, conductivity, and settleability were measured and compared to sludge microbial community structure, determined by microscopic observations and high-throughput 16S rDNA sequencing. The high doxycycline concentration negatively impacted settleability and correlated with a decrease in bacterial diversity and floc disintegration. The addition of doxycycline promoted the enrichment of Proteobacteria Brevundimonas sp., Luteibacter anthropi, and the Bacteroidetes Chryseobacterium massoliae. These species are known to be resistant to a wide spectrum of antibiotics, including tetracyclines. A study of a larger scale may be conducted based on this study’ results.

Evaluating membrane bioreactor treatment for the elimination of emerging contaminants using different analytical methods

Since wastewater treatment plants (WWTPs) were not originally designed to eliminate contaminants of emerging concern (CECs), alternative strategies like membrane bioreactor (MBR) technology are gaining importance in achieving effective CEC removal and minimising their environmental impact. In this study, composite wastewater samples were collected from the biggest WWTP in the Basque Country (Galindo, Biscay) and the performance of two secondary treatments (i.e. conventional activated sludge treatment, CAS, and MBR) was assessed. The combination of a suspect screening approach using liquid chromatography tandem high-resolution mass spectrometry (LC-HRMS) and multitarget analysis by gas chromatography-mass spectrometry (GC-MS) allowed the detection of approximately 200 compounds in the WWTP effluents. The estimated removal efficiencies (REs) revealed that only 16 micropollutants exhibited enhanced removal by MBR treatment (RE > 70% or 40 – 60%). The environmental risk posed by the non-eliminated compounds after both treatments remained similar, being anthracene, clarithromycin, bis(2-ethylhexyl) phthalate (DEHP) and dilantin the most concerning pollutants (RQ > 1). The Microtox® bioassay confirmed the MBR’s efficiency in removing baseline toxicity, while suggesting a similar performance of CAS treatment. These minimal differences between treatments call into question the worthiness of MBR treatment and emphasise the need to seek more efficient alternative treatment methods.

Effects of Wastewater Treatment Processes on the Removal Efficiency of Microplastics Based on Meta-analysis

Microplastics (MPs) are ubiquitous emerging pollutants that have been found in the marine, freshwater, air, and soil environments. Wastewater treatment plants (WWTPs) play an important role in releasing MPs to the environment. Therefore, understanding the occurrence, fate, and removal mechanism of MPs in WWTPs is of great importance towards microplastic control. In this review, the occurrence characteristics and removal rates of MPs in 78 WWTPs from 57 studies were discussed based on Meta-analysis. Specifically, the key aspects regarding MPs removal in WWTPs, such as wastewater treatment processes and MPs shapes, sizes, and polymer compositions were analyzed and compared. The results showed that:① the abundances of MPs in the influent and effluent were 1.56×10-2-3.14×104 n·L-1 and 1.70×10-3-3.09×102 n·L-1, respectively. The abundance of MPs in the sludge ranged from 1.80×10-1 to 9.38×103 n·g-1. ② The total removal rate (>90%) of MPs by WWTPs using oxidation ditch, biofilm, and conventional activated sludge treatment processes was higher than that using sequencing batch activated sludge, anaerobic-anoxic-aerobic, and anoxic-aerobic processes. ③ The removal rate of MPs in primary, secondary, and tertiary treatment process were 62.87%, 55.78%, and 58.45%, respectively. The combination process of "grid+ sedimentation tank+primary sedimentation tank" had the highest removal rate towards MPs in primary treatment processes, and the membrane bioreactor had the highest one beyond other secondary treatment processes. Filtration was the best process in tertiary treatment. ④ The film, foam, and fragment MPs were easier to remove (>90%) than fiber and spherical (<90%) MPs by WWTPs. The MPs with particle size larger than 0.5 mm were easier to remove than those with particle size smaller than 0.5 mm. The removal efficiencies of polyethylene (PE), polyethylene terephthalate (PET), and polypropylene (PP) MPs were higher than 80%.

Risk ranking of macrolide antibiotics – Release levels, resistance formation potential and ecological risk

Antibiotic resistance (AR) development in natural water bodies is a significant source of concern. Macrolide antibiotics in particular have been identified as pollutants of concern for AR development throughout the literature, as well as by state and international authorities. This study utilises a probabilistic model to examine the risk of AR development arising from human-use macrolide residues, utilising administration rates from Ireland as a case study. Stages modelled included level of administration, excretion, degradation in wastewater, removal in wastewater treatment, assuming conventional activated sludge (CAS) treatment, and dilution. Release estimates per day, as well as risk quotient values for antibiotic resistance development and ecological impact, are generated for erythromycin, clarithromycin, and azithromycin. In the modelled scenario in which conventional activated sludge treatment is utilised in wastewater treatment, this model ranks risk of resistance development for each antibiotic in the order clarithromycin > azithromycin > erythromycin, with mean risk quotient values of 0.50, 0.34 and 0.12, respectively. A membrane bioreactor scenario was also modelled, which reduced risk quotient values for all three macrolides by at least 50 %. Risk of ecological impact for each antibiotic was also examined, by comparing environmental concentrations predicted to safety limits based on toxicity data for cyanobacteria and other organisms from the literature, with azithromycin being identified as the macrolide of highest risk. This study compares and quantifies the risk of resistance development and ecological impact for a high-risk antibiotic group in the Irish context, and demonstrates the potential for risk reduction achieved by adoption of alternative (e.g. membrane bioreactor) technology.

Antibiotic adsorption by natural and modified clay minerals as designer adsorbents for wastewater treatment: A comprehensive review

Increased antibiotic use worldwide has become a major concern because of their health and environmental impacts. Since most antibiotic residues can hardly be removed from wastewater using conventional treatments, alternative methods receive great attention. Adsorption is one of the most efficient and cost-effective treatment methods for antibiotics. Among the adsorbents, clay minerals have garnered increasing attention due to their unique properties including availability, high specific surface area, low cost, cation exchange capacity, and good removal efficiency. This paper reviews the recent progress made in the use of natural and modified clay minerals for the removal of antibiotics from water. First, the sources, occurrence, removal and health effects of the antibiotics commonly encountered in water bodies are described. Antibiotic concentration levels and average removal efficiencies measured in conventional activated sludge treatment systems worldwide are also provided to better address the problem. Second, the review explores the characteristics of clay minerals as adsorbent of antibiotics and the factors affecting the adsorption. The review identifies and discusses the future trends and strategies used to increase the adsorption capacity of clay minerals by modification and combination techniques (intercalation of novel functional groups such as organocations, biopolymers and metal pillared-clay minerals, combination with biochar or thermal activation). The quantitative comparisons of clay minerals' ability for antibiotic removal are given. Some natural clay minerals have good removal potential for antibiotics, with maximum adsorption capacities over 100mg/g. For most other adsorbents, surface modifications and combination techniques resulted in improved adsorption properties (including higher surface area, enhanced adsorption capacity, increased stability and mechanical strength). Finally, the application of these adsorbents at pilot scale, using real wastewater samples, their reuse, economic analysis and life cycle assessment are other issues that have been considered.

Advanced wastewater treatment with ozonation and granular activated carbon filtration: Inactivation of antibiotic resistance targets in a long-term pilot study

The inactivation of antibiotic resistant bacteria (ARB) and genes (ARGs) in an advanced plant combining ozonation and granular activated carbon (GAC) filtration applied for effluent after conventional activated sludge treatment at a full-scale urban wastewater treatment plant was investigated for over 13 consecutive months. The nitrite compensated specific ozone dose ranged between 0.4 and 0.7 g O3/g DOC with short-time sampling campaigns (0.2–0.9 g O3/g DOC). Samples were analysed with culture-dependent methods for bacterial targets and with qPCR for genes. The log removal values were correlated with a decrease of the matrix UV absorption at 254 nm (ΔUV254) and indicated a range of ΔUV254 that corresponds to a sufficient membrane damage to affect DNA. For trimethoprim/sulfamethoxazole resistant E. coli, sul1, ermB and tetW, this phase was observed at ΔUV254 of ~30 % (~0.5 g O3/g DOC). For ampicillin resistant E. coli and blaTEM-1, it was observed around 35–40 % (~0.7 g O3/g DOC), which can be linked to mechanisms related to oxidative damages in bacteria resistant to bactericidal antibiotics. GAC treatment resulted in a further abatement for trimethoprim/sulfamethoxazole E. coli, sul1 and tetW, and in increase in absolute and relative abundance of ermB and blaTEM-1.

Applying novel methods in conventional activated sludge plants to treat low-strength wastewater

Conventional activated sludge system is confidently widely used for biological treatment plants of municipal wastewater but suffering from operation problems that affect their efficiencies and effluent qualities, especially when treating low-strength wastewater with increasing incoming flow. The objective of this study is to evaluate and compare the novel methods used in upgrading conventional activated sludge treatment systems receiving low-strength wastewater to generate good effluent quality. GPS-X Simulator V 8.0 was used for model calibration and plant performance prediction. The calibrated GPS-X model proved that eliminating primary settling from the treatment process does not affect BOD5 and COD removal, while TSS removal is decreased, and NH4-N removal is increased. Increasing the return activated sludge flow from 50 to 150% of influent flow does not affect conventional activated sludge process, while the change of waste activated flow had a vital effect on process performance. The presence of an anoxic zone in conventional activated sludge processes treating low-strength wastewater has no significant impact on plant performance. Also, the model outputs proved that adding filling media to the aeration tank was able to handle an increase of influent flow and a stable performance of BOD5, and NH4-N removal was observed.

Effect of ozonation on the biodegradability of urban wastewater treatment plant effluent

The present work aimed to study the effect of ozonation on the organic sum parameters linked to enhanced biodegradability. Laboratory experiments were conducted with the effluent of four Austrian urban wastewater treatment plants with low food to microorganism ratios and different matrix characteristics. Biochemical oxygen demand over 5 days (BOD5) was measured before ozonation and after application of different specific ozone doses (Dspec) (0.4, 0.6 and 0.8 g O3/g DOC). Other investigated organic parameters comprised chemical oxygen demand (COD), dissolved organic carbon (DOC), UV absorption at 254 nm (UV254), which are parameters that are applied in routine wastewater analysis. Carbamazepine and benzotriazole were measured as reference micropollutants. The results showed a dose-dependent increase in biological activity after ozonation; this increase was linked to the enhanced biodegradability of substances that are recalcitrant to biodegradation in conventional activated sludge treatment. The highest relative change was determined for BOD5, which already occurred between 0 and 0.4 g O3/g DOC for all samples. Increasing the Dspec to 0.6 and 0.8 g O3/g DOC resulted in a less pronounced increase. DOC was not substantially decreased after ozonation, which was consistent with a low reported degree of mineralization, while partial oxidation led to a quantifiable decrease in COD (7 to 17%). Delta UV254 and the decline in specific UV absorption after ozonation clearly correlated with Dspec. In contrast, for COD and biodegradable DOC (BDOC), a clear dose-response pattern was identified only after exposure to BOD5 measurement. Indications for improved biodegradability were further supported by the rise in the BOD5/COD ratio. The results indicated that subsequent biological processes have a higher degradation potential after ozonation. The further reduction in biodegradable organic carbon emission by the combination of ozonation and biological post treatment represents another step towards sustainable water resource management in addition to micropollutant abatement.

Reduction of Cost and Environmental Impact in the Treatment of Textile Wastewater Using a Combined MBBR-MBR System.

A hybrid Moving Bed Biofilm Reactor—Membrane Bioreactor (MBBR-MBR) was developed for the treatment of wastewater from a Spanish textile company. Compared with conventional activated sludge (CAS) treatment, the feasibility of this hybrid system to reduce economic and environmental impact on an industrial scale was conducted. The results showed that, technically, the removal efficiency of COD, TSS and color reached 93%, 99% and 85%, respectively. The newly dyed fabrics performed with the treated wastewater were qualified under the standards of the textile industry. Economically, the values of Capital Expenditure (CAPEX) calculated for the hybrid MBBR-MBR system are profitable because of the reduction in Operational Expenditure (OPEX) when compared with CAS treatment, due to the lower effluent discharge tax thanks to the higher quality of the effluent and the decolorizing agent saved. The result of Net Present Value (NPV) and the Internal Rate of Return (IRR) of 18% suggested that MBBR-MBR is financially applicable for implantation into the industrial scale. The MBBR-MBR treatment also showed lower environmental impacts than the CAS process in the life cycle assessment (LCA) study, especially in the category of climate change, thanks to the avoidance of using extra decolorizing agent, a synthetic product based on a triamine.

Analysis of pharmaceuticals, hormones and bacterial communities in a municipal wastewater treatment plant – Comparison of parallel full-scale membrane bioreactor and activated sludge systems

In this study, the occurrence of pharmaceuticals, hormones and bacterial community structures was studied at a wastewater treatment plant in Finland having two different parallel treatment lines: conventional activated sludge (CAS) treatment with a sedimentation stage, and a membrane bioreactor (MBR). Influent and effluents were sampled seven times over a period of one year. The bacterial communities of the influent samples showed a high degree of similarity, except for the February sample which had substantially lower diversity. There was significant fluctuation in the species richness and diversity of the effluent samples, although both effluents showed a similar trend. A marked decrease in diversity was observed in effluents collected between August and November. The initiation of nitrogen removal as a result of an increase in temperature could explain the changes in microbial community structures. In overall terms, suspended solids, bacteria and total organic matter (COD and BOD) were removed to a greater extent using the MBR, while higher Tot-N, Tot-P and nitrate removal rates were achieved using the CAS treatment. Estrone (E1) concentrations were also consistently at a lower level in the MBR effluents (<0.1–0.68 ng/l) compared to the CAS effluents (1.1–12 ng/l). Due to the high variation in the concentrations of pharmaceuticals, no clear superiority of either process could be demonstrated with certainty. The study highlights the importance of long-term sampling campaigns to detect variations effectively.

A Review on Occurrence and Spread of Antibiotic Resistance in Wastewaters and in Wastewater Treatment Plants: Mechanisms and Perspectives.

This paper reviews current knowledge on sources, spread and removal mechanisms of antibiotic resistance genes (ARGs) in microbial communities of wastewaters, treatment plants and downstream recipients. Antibiotic is the most important tool to cure bacterial infections in humans and animals. The over- and misuse of antibiotics have played a major role in the development, spread, and prevalence of antibiotic resistance (AR) in the microbiomes of humans and animals, and microbial ecosystems worldwide. AR can be transferred and spread amongst bacteria via intra- and interspecies horizontal gene transfer (HGT). Wastewater treatment plants (WWTPs) receive wastewater containing an enormous variety of pollutants, including antibiotics, and chemicals from different sources. They contain large and diverse communities of microorganisms and provide a favorable environment for the spread and reproduction of AR. Existing WWTPs are not designed to remove micropollutants, antibiotic resistant bacteria (ARB) and ARGs, which therefore remain present in the effluent. Studies have shown that raw and treated wastewaters carry a higher amount of ARB in comparison to surface water, and such reports have led to further studies on more advanced treatment processes. This review summarizes what is known about AR removal efficiencies of different wastewater treatment methods, and it shows the variations among different methods. Results vary, but the trend is that conventional activated sludge treatment, with aerobic and/or anaerobic reactors alone or in series, followed by advanced post treatment methods like UV, ozonation, and oxidation removes considerably more ARGs and ARB than activated sludge treatment alone. In addition to AR levels in treated wastewater, it examines AR levels in biosolids, settled by-product from wastewater treatment, and discusses AR removal efficiency of different biosolids treatment procedures. Finally, it puts forward key-points and suggestions for dealing with and preventing further increase of AR in WWTPs and other aquatic environments, together with a discussion on the use of mathematical models to quantify and simulate the spread of ARGs in WWTPs. Mathematical models already play a role in the analysis and development of WWTPs, but they do not consider AR and challenges remain before models can be used to reliably study the dynamics and reduction of AR in such systems.

Simultaneous sludge minimization, pollutant and nitrogen removal using integrated MBBR configuration for tannery wastewater treatment

An advanced operational configuration of anoxic-aerobic moving bed biofilm reactors (AMOMOX process) was experimentally demonstrated to achieve simultaneous sludge yield minimization, pollution and nitrogen removal. The AMOMOX experimentation witnessed considerable variation in process parameters while feed operation changed from synthetic wastewater to real tannery influent. The strict maintenance of operational strategies resulted prominent removal of TCOD, SCOD, ammonia nitrogen and total nitrogen higher upto 93.5%, 94.8%, 95.2% and 88.7% respectively. The nourishment of filamentous microbiota and purposeful promotion of cell-lysis effectively sustained sludge yield restriction. Here, the sludge yield (Yobs) lowering upto 0.51 gVSS/gCOD ultimately turned an overall sludge minimization of 46.8% compared with a parallel-run conventional activated sludge treatment. The observations were further supported by sophisticated instrumental imaging, thermogravimetric analysis and batch digestion test of the sludge pool. The experimental Yobs and corresponding solids retention showed consensus with the reported correlation model and, thus, a modified correlation was tested.

Integrating photobioreactor with conventional activated sludge treatment for nitrogen removal from sidestream digestate: Current challenges and opportunities

Sidestream digestate is a liquid effluent stream generated from the anaerobic sludge digestion process in a wastewater treatment plant (WWTP). It is rich in inorganic nutrients, mainly nitrogen and phosphorus, and it requires further treatment in order to meet the stringent discharge/regulatory standards. It is usually treated through conventional activated sludge process (ASP) in the WWTPs. However, its direct recirculation to the WWTP imparts burden of excess nutrient removal on the biological treatment, i.e. in terms of the increased energy costs for aeration and it can pose a higher risk of eutrophication in the receiving water body. One alternative is the integration of photobioreactor (PBR) with ASP treatment for enhanced nitrogen removal from sidestream digestate. This paper critically reviewed the techno-economic feasibility of integrating a PBR with the ASP of a WWTP for sidestream digestate treatment. It is estimated that the integrated PBR-ASP process requires small land area and can achieve high nitrogen removal. Microalgae, owing to its high ability in nutrient cycling and biomass production, can effectively be used as biocatalysts for the treatment of sidestream digestate. Microalgae can assimilate nitrogen resulting in oxygen production by photosynthesis. The heterotrophic bacteria mineralise the pollutants present in wastewater, i.e. in the presence of oxygen, releasing carbon dioxide through bacterial respiration. The carbon dioxide is utilised by the microalgae to complete the photosynthetic cycle for wastewater treatment, thus decreasing the greenhouse emissions. The microalgae-bacterial treatment as compared to ASP can reduce more than 50% of the energy costs.

Treatment of a Biological Effluent Containing Metronidazole

Emerging pollutants like metronidazole (MNZ) affect the removal efficiency of conventional activated sludge (CAS) based wastewater treatment plants and can escape traditional treatment facilities. Advanced oxidation processes (AOPs) and membrane filtration could complement the existing processes to completely eliminate pollutants of emerging concern in wastewaters. This work investigated the application of photochemical processes for treating a synthetic wastewater effluent from CAS treatment and membrane filtration of the biological effluents after CAS experiments with MNZ, caffeine (CAF) and ibuprofen (IBU). Photochemical experiments were conducted in a batch photochemical reactor. The influence of different chemical species such as H2O2, Fe(II), K2S2O8 and TiO2 and of pH value in the system were studied in terms of TOC removal and MNZ degradation. The application of UV/K2S2O8/Fe(II) resulted in 78 % TOC removal in the effluent. Complete degradation of MNZ was observed after 30 min of treatment in the following experiments: UV/H2O2/Fe(II) at pH 3 and UV/K2S2O8. Moreover, the effluents were treated by means of a membrane bioreactor (MBR). Polycarbonate track-etch membranes of 50 and 100 nm pore size were used. The results obtained showed that the carbon removal by the MBR was comparable with the conventional biological treatment. Rejection of MNZ depends on the presence/absence of other emerging contaminants in the wastewater effluents.

First comparison of conventional activated sludge versus root-zone treatment for SARS-CoV-2 RNA removal from wastewaters: Statistical and temporal significance

In the initial pandemic phase, effluents from wastewater treatment facilities were reported mostly free from Severe Acute Respiratory Coronavirus 2 (SARS-CoV-2) RNA, and thus conventional wastewater treatments were generally considered effective. However, there is a lack of first-hand data on i) comparative efficacy of various treatment processes for SARS-CoV-2 RNA removal; and ii) temporal variations in the removal efficacy of a given treatment process in the backdrop of active COVID-19 cases. This work provides a comparative account of the removal efficacy of conventional activated sludge (CAS) and root zone treatments (RZT) based on weekly wastewater surveillance data, consisting of forty-four samples, during a two-month period. The average genome concentration was higher in the inlets of CAS-based wastewater treatment plant (WWTP) in the Sargasan ward (1.25 × 103 copies/ L), than that of RZT-based WWTP (7.07 × 102 copies/ L) in an academic institution campus of Gandhinagar, Gujarat, India. ORF 1ab and S genes appeared to be more sensitive to treatment i.e., significantly reduced (p < 0.05) than N genes (p > 0.05). CAS treatment exhibited better RNA removal efficacy (p = 0.014) than RZT (p = 0.032). Multivariate analyses suggested that the effective genome concentration should be calculated based on the presence/absence of multiple genes. The present study stresses that treated effluents are not always free from SARS-CoV-2 RNA, and the removal efficacy of a given WWTP is prone to exhibit temporal variability owing to variations in active COVID-19 cases in the vicinity and genetic material accumulation over the time. Disinfection seems less effective than the adsorption and coagulation processes for SARS-CoV-2 removal. Results stress the need for further research on mechanistic insight on SARS-CoV-2 removal through various treatment processes taking solid–liquid partitioning into account.

Effects of hydrothermal treatments on destruction of per- and polyfluoroalkyl substances in sewage sludge

Sewage sludge has become a sink of per- and polyfluoroalkyl substances (PFAS) due to the ineffectiveness of PFAS removal during conventional activated sludge treatment process. In this study, we evaluated the performance of an enhanced method for PFAS extraction from sewage sludge. Significant matrix effect was observed for samples derived from untreated and hydrothermally treated sludge. Extra steps for removing potential interferences were thus needed to reduce these matrix effects and improve the accuracy of PFAS quantification. Hydrothermal treatment at 165 °C for 0.5/2 h and 250 °C for 0.5 h increased the concentration of extractable PFAAs in treated sludge. Increasing the temperature to 300 °C resulted in complete degradation of PFCAs after hydrothermal processing, but still increased the concentrations of PFSAs and PFAA precursors. The concentration increase could be due to the conversion of PFAA precursors to PFAAs and the release of PFAAs from sewage sludge during thermal treatment. Ca(OH)2 addition to hydrothermal treatment completely removed PFAA precursors but significantly increased the extractable PFAAs, except PFHpA and PFHxS, at 165 °C and all PFSAs at 300 °C. This study revealed the difficulties in extracting and quantifying PFAS in sludge and demonstrated the need for further research on finding suitable solutions for complete removal or destruction of PFAS in highly heterogeneous sewage sludge.

Effect of pH on nutrient removal and crop production of hydroponic systems treating brewery effluent

Abstract The use of a crop to remove nutrients from brewery effluent and the influence of pH on these removal rates was evaluated. Cabbage (Brassica oleracea) was grown in recirculating hydroponic systems fed with post-anaerobically digested brewery effluent (BE) either subject to pH adjustment (6.5–7.0) or unaltered pH (8.0–8.5). These were compared with cabbages grown in water only and in a inorganic fertiliser nutrient solution (NS). Hydroponic systems fed with pH adjusted BE removed significantly more nitrogen and phosphorus than systems fed with pH unadjusted BE (p &amp;lt; 0.05). The final weight of cabbages from the pH adjusted BE systems were 6.7 times greater than cabbages from the pH unadjusted BE systems, whereas pH adjustment had no influence on cabbage weight in the water-only and NS treatments. Anaerobically digested BE that is not pH adjusted is not a suitable water and nutrient source for the hydroponic production of cabbages. However, pH adjustment of BE renders it more suitable for hydroponic crop production with hydroponic systems decreasing dissolved inorganic nitrogen, ammonium, phosphate and chemical oxygen demand concentrations by 72.8, 31.8, 98.5 and 51.0%, respectively. Hydroponic systems can be used to treat post-anaerobically digested BE to a similar standard obtained by conventional activated sludge treatment system.