Milk Concentrations Research Articles

Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flock

The objectives of this study were to estimate genetic parameters for citric acid content (CA) and lactic acid content (LA) in sheep milk and to identify the associated candidate genes in a New Zealand dairy sheep flock. Records from 165 ewes were used. Heritability estimates based on pedigree records for CA and LA were 0.65 and 0.33, respectively. The genetic and phenotypic correlations between CA and LA were strong-moderate and negative. Estimates of genomic heritability for CA and LA were also high (0.85, 0.51) and the genomic correlation between CA and LA was strongly negative (-0.96 ± 0.11). No significant associations were found at the Bonferroni level. However, one intragenic SNP in C1QTNF1 (chromosome 11) was associated with CA, at the chromosomal significance threshold. Another SNP associated with CA was intergenic (chromosome 15). For LA, the most notable SNP was intragenic in CYTH1 (chromosome 11), the other two SNPs were intragenic in MGAT5B and TIMP2 (chromosome 11), and four SNPs were intergenic (chromosomes 1 and 24). The functions of candidate genes indicate that CA and LA could potentially be used as biomarkers for energy balance and clinical mastitis. Further research is recommended to validate the present results.

The Effects of Two Selected Single Nucleotide Polymorphisms of the Fatty Acid Synthase Gene on the Fat Content and Fatty Acid Profile of Cow's Milk from the Polish Holstein-Friesian Red-and-White Breed versus Two Polish Red-and-White and Polish Red Conservation Breeds Kept in Poland.

Fatty acid synthase (FASN) is a metabolic enzyme responsible for the synthesis of fatty acids in milk and meat. The SNPs g.841G/C and g.17924A/G of the FASN gene significantly influence the fat and fatty acid content of milk from cows of various breeds. Therefore, these SNPs were selected for this study. This study aimed to analyze the relationship of SNPs and their genotypes with the fat content and fatty acid profile of milk from Polish Red-and-White (ZR), Polish Red (RP), and Polish Holstein-Friesian Red-and-White (RW) cows. Milk samples were obtained during a milking trial. SNP genotyping was performed using the real-time PCR (HRM) method. It was shown that SNPs (with specific genotypes) were significantly associated with the presence of fatty acids such as C18:1n9t and C18:2n6c in milk. In addition, it was found that the milk fat from the ZR (genotypic variant A/G, AA) and RP (genotypic variant GG, A/G) breeds often exhibited a more attractive fatty acids profile than the milk fat from RW cows. This information can be used by both cattle breeders and people interested in consuming functional foods.

Bioactive Peptides in Dairy Milk: Highlighting the Role of Melatonin.

Melatonin, an endogenous indolamine derived from tryptophan, is primarily synthesized by the pineal gland in mammals and regulated by a complex neural system. Its release follows a circadian rhythm, which is crucial for regulating physiological processes in response to light-dark cycles in both humans and animals. In this review, we report that the presence of this hormone in bovine milk, with significant differences in concentration between daytime and nighttime milking, has increased interest in milk as a natural source of bioactive molecules. Melatonin lowers cortisol levels at night, reduces body temperature and blood pressure, coinciding with decreased alertness and performance, acts as an antioxidant and anti-inflammatory agent, modulates the immune system, offers neuroprotective benefits, and supports gastrointestinal health by scavenging free radicals and reducing oxidative stress in dairy cows. Many factors influence the release of melatonin, such as the intensity of artificial lighting during nighttime milking, the frequency of milkings, milk yield, and genetic differences between animals. Nocturnal milking under low-intensity light boosts melatonin, potentially reducing oxidative damage and mastitis risk. Additionally, ultra-high temperature (UHT) treatment does not significantly affect the melatonin content in milk. However, further research on its stability during milk processing and storage is crucial for ensuring product efficacy. In some countries, nighttime milk with naturally elevated melatonin content is already commercialized as a natural aid for sleep. Thus, naturally melatonin-rich milk may be a promising alternative to synthetic supplements for promoting better sleep and overall well-being.

Association Between Prenatal Exposure to Per- and Poly-Fluoroalkyl Substances From Electronic Waste Disassembly Areas and Steroid Hormones in Human Milk Samples.

Per- and poly-fluoroalkyl substances (PFAS), which are long-lasting environmental contaminants that are released into the environment during the e-waste disassembly process, pose a threat to human health. Human milk is a complex and dynamic mixture of endogenous and exogenous substances, including steroid hormones and PFAS. Therefore, in this study, we aimed to investigate the association between PFAS and steroid hormones in human milk from women living close to an e-waste disassembly area. In 2021, we collected milk samples from 150 mothers within 4weeks of delivery and analyzed them via liquid chromatography-tandem mass spectrometry to determine the levels of 21 perfluorinated compounds and five steroid hormones (estrone, estriol, testosterone, progesterone, and androstenedione [A-dione]). We also performed multiple linear regression analysis to clarify the association between maternal PFAS exposure and steroid hormone concentrations. Our results indicated that PFOA and PFOS were positively associated with estrone (β, 0.23; 95% CI, 0.08-0.39) and A-dione (β, 0.186; 95% CI, 0.016-0.357) concentrations in human milk, respectively. Further, the average estimated daily intake of PFOA and PFOS were 36.5ng/kgbw/day (range, 0.52-291.7ng/kgbw/day) and 5.21ng/kgbw/day (range, 0.26-32.3ng/kgbw/day), respectively. Of concern, the PFAS intake of breastfeeding infants in the study area was higher than the recommended threshold. These findings suggested that prenatal exposure to PFAS from the e-waste disassembly process can influence steroid hormones levels in human milk. Increased efforts to mitigate mother and infant exposure to environmental pollutants are also required.

Vitamin D3 formation in milk by UV treatment – Novel insights into a rediscovered process

Vitamin D3 is essential for several functions in the human body and the demand is usually covered by natural reactions in skin with UV radiation delivered by the sun. But living beyond a latitude of 35° can lead to a lack of sufficient exposition to the deciding wavelength. Here, many countries fortify their milk prophylactically with artificial vitamin D3. However, the precursor molecule of vitamin D3 (7-deydrocholesterol) is already naturally located in the milk fat globule membrane. Thus, this study deals with the transformation of the naturally occurring 7-dehydrocholesterol into vitamin D3 through UV treatment of the milk - a mechanism that was observed a century ago only indirectly. Different parameters such as temperature (10 - 50°C), fluid flow regimen (turbulent vs. laminar thin film, i.e., 0.6 mm) and wavelength (254, 280 and 313 nm) were investigated in this study for their efficiencies. The UV dose of each experiment was measured with chemical actinometry delivering the actually applied dose reaching the milk. Thus, the connection between applied UV dose and generated vitamin D3 content in the milk measured quantitively with LC-MS/MS was evaluated here that both were not possible a hundred years ago. The experimental results revealed that temperature generally promotes the vitamin D3 formation at 254 nm. Further, a turbulent flow is not as efficiently treated as a laminar thin film flow that is as narrow as 0.6 mm. As expected from absorbance spectra of the precursor molecule 7-dehydrocholesterol, 280 nm turned out to be the most efficient wavelength, followed by intermediate success through irradiation with 254 nm and almost no effect by 313 nm. Generally, it was shown that vitamin D3 concentration of milk was easily increased by UV treatment with today's technologies and that adjustment of certain physical parameters have a significant effect on the efficiency.

Influence of outbreeding and inbreeding on milk productivity of the Kostroma breed cows

The material about modern genealogical structure of the Kostroma breed of cattle, breeding base and kinship relations of cows in the breeding farms of the Kostroma region is presented. Domestic Kostroma breed of cattle, possessing valuable economically useful qualities, accounts for 33.5% in the region. The studies were conducted in three breeding farms and two pedigree breeding units in 2023. Breeding and zootechnical records data were used as the research material. The research methods are zootechnical, population-genetic and analytical with the use of computer programs. Milk productivity of cows at outbreeding (unrelated breeding) and at different degrees of kinship at inbreeding was analyzed separately for all breeding plants and pedigree breeding units in the Kostroma region. Out of 1736 cows of the Kostroma breed studied, 918 (52.9%) were obtained by outbreeding, 818 (47.1%) by inbreeding, including distant – 461 (26.5%), moderate – 310 (17.8%), close – 41 (2.4%) and consanguinity – 6 (0.4%). Outbreeding and distant and moderate inbreeding were mostly used in breeding farms. The level of spontaneous inbreeding was 2.8%. Higher milk yields are inherent to outbred cows from all breeding farms of the Kostroma region, fat and protein content in milk - to outbred cows and the animals with different degrees of inbreeding in the conditions of specific farms. The results of the research indicate higher milk productivity of the cows under outbreeding. The obtained data were used for making plans of selection and breeding work with the Kostroma breed of cattle in breeding farms.

The Effect of Mungbeans and Skim Milk Concentration on The Nutritional and Sensory Properties of Corn Milk

The research aims to determine the effect of adding mungbean and skim milk on the physical, chemical and sensory properties of corn milk as well as the best formula to produce the best corn milk. The experimental design used was a factorial randomized block design. The factors studied were the addition of green beans consisting of three levels, namely 10, 20 and 30% and the concentration of skim milk consisting of three levels, namely 3, 9 and 15%. Three repetitions were carried out to obtain 27 experimental units. The results showed that the higher the addition of green beans, the lower the viscosity and protein content. The higher the concentration of skim milk used, the higher the total dissolved solids, the lower the water content, and the higher the fat content. Determining the best treatment using the Multiple Attribute Method. Sweet corn milk with the addition of 10% green beans and 3% skim milk was the best treatment with total dissolved solids of 11.67oBrix; viscosity 27.9 cP; moisture 86.1%; ash content 0.357%; fat content 0.643%; protein content 1.69; whitish yellow color (3.35); slightly strong corn aroma (3.28); slightly thick (3.23); delicious flavor (3.67); and favorable (3.63).

Changes in the Fatty Acid Profile of Lactating Women Living in Poland-A Comparison with the Fatty Acid Profile of Selected Infant Formulas.

The present study examined the fatty acid content of human milk from Polish women living in the Warmia and Mazury region with regard to different lactation periods and compared it with the fatty acid content of selected infant formulas. The analysis included samples of breast milk-colostrum (n = 21), transitional milk (n = 26), and mature milk (n = 22). Fat was extracted using the Rose-Gottlieb method, and the fatty acid profile was determined by gas chromatography with a flame ionization detector (FID). The proportion of SFAs (saturated fatty acids) > MUFAs (monounsaturated fatty acids) > PUFAs (polyunsaturated fatty acids) was determined in each fraction of breast milk and infant formula. Palmitic, oleic, and linoleic acids predominated in breast milk and infant formulas. Colostrum contained lower contents of selected SFAs (caprylic, capric, lauric) and higher contents of selected MUFAs (ercucic) and PUFAs (arachidonic and docosahexaenoic) (p < 0.05) relative to transitional and mature milk. Infant formulas were distinguished from human milk in terms of their SFA (caproic, caprylic, lauric, arachidic), MUFA (oleic), and PUFA (linoleic, α-linoleic) content. It should be noted that infant formulas contained significantly lower trans fatty acid (TFA) content-more than thirty-six and more than nineteen times lower than in human milk. Furthermore, human milk contained branched-chain fatty acids (BCFAs) at 0.23-0.28%, while infant formulas contained only trace amounts of these acids. The average ratio of n-6 to n-3 fatty acids for human milk was 6.59:1 and was close to the worldwide ratio of 6.53 ± 1.72:1. Both principal component analysis (PCA) and cluster analysis (CA) indicated significant differences in the fatty acid profile relative to lactation and a different profile of infant formulas relative to breast milk.

Potential of 2 Northern European brown seaweeds (Fucus serratus and Fucus vesiculosus) as enteric methane inhibitors in dairy cows.

The 2 brown seaweeds, Fucus serratus and Fucus vesiculosus, have demonstrated anti-methanogenic properties in vitro with reductions in CH4 production ranging from 53 to 63%. This study aimed to investigate the effects of the 2 Fucus seaweeds on enteric CH4 emissions, DMI, ECM, and nutrient digestibility when fed to dairy cows. The experiment was conducted using 4 multi-cannulated lactating Danish Holstein dairy cows, which over 3 experimental periods received either: 1) basal diet (CON; diet without any seaweed), 2) basal diet diluted with 4% (DM basis) Fucus serratus (SER), or 3) basal diet diluted with 4% (DM basis) Fucus vesiculosus (VES); resulting in one complete 3 × 3 Latin square and one incomplete 3 × 3 Latin square. Each period lasted 21 d and consisted of 14 d of adaptation, followed by 3 d of digesta sampling, and 4 d of gas exchange measurements using respiration chambers. Milk yield and feed intake were recorded daily. Blood samples were collected on d 15 and 17. All parameters were statistically analyzed using a mixed procedure of R. Opposite to what we had expected, neither of the 2 Fucus seaweeds reduced CH4 emissions from the dairy cows as daily CH4 production was significantly higher for both Fucus treatments compared with CON. Additionally, CH4 yield (g CH4/kg DMI) and intensity (g CH4/kg ECM) were significantly higher for SER compared with CON. Milk yield, DMI, and total-tract digestibility were unaffected by the treatments; however, SER resulted in lower milk protein yield (kg/d) and lower milk and blood plasma urea concentrations compared with CON. In conclusion, neither Fucus serratus (SER) nor Fucus vesiculosus (VES) showed potential as methane-mitigating feed additives when fed to dairy cows at an inclusion level of 4% of DM. The inclusion of the 2 brown seaweeds had no effects on DMI, milk yield, or total-tract digestibility.

Physicochemical characteristics of jack bean (Canavalia ensiformis (L.) DC.) milk, a non-dairy milk alternative developed using various pretreatment methods

The endeavor to turn jack bean (Canavalia ensiformis (L.) DC.) into jack bean milk faced a challenge regarding its hard skin, which makes pretreatment of the jack bean before processing essential to soften and ease the dehulling of beans, as well as aid the grinding process. However, different pretreatment methods influence the characteristics of treated jack beans and jack bean milk as the final product. This study, thus, aimed to investigate the effect of different jack bean pretreatment methods such as soaking in NaHCO3 solution (P1), soaking in hot water (95℃) (P2), and a combination of boiling and soaking in water (P3), on the physicochemical properties of jack bean milk. Jack bean milk was prepared by subjecting jack beans to pretreatment, dehulling, wet grinding, filtering, and heating before proceeding with analyses based on proximate composition, phase behavior, color and viscosity. The results demonstrated that P2 produced the highest protein (7.01±0.11%), fat (0.10±0.00%), and ash contents (0.11±0.00%) of jack bean milk. P1 yielded higher protein and ash contents of jack bean milk than that of P3, while no significant differences in fat contents were discovered in both P1 and P3. Besides, a slight difference in carbohydrate contents was found among all treatments, ranging from 1.17 to 1.20%. The phase behavior and color analyses showed P1 and P2 to significantly generate higher colloidal stability and whiteness index value of jack bean milk than P3. In addition, jack bean milk from P2 possessed the highest viscosity value of 48.1±1.4 cP, followed by P1 (28.6±0.6 cP) and P3 (12.6±0.5 cP). Accordingly, the recent study proposes the pretreatment of soaking in hot water (95℃) to be applied in jack bean milk processing and provides new insights into how pretreatment methods affect the characteristics of plantbased milk products.

Is the milk we drink safe from elevated concentrations of prioritised heavy metals/metalloids? – A global systematic review and meta-analysis followed by a cursory risk assessment reporting

Milk has been globally recognised as a comprehensive and vital food source for centuries. However, the presence of heavy metals and metalloids (metal(loid)s) in milk is a global problem. As metal(loid)s are present in the soil due to natural geogenic and various anthropogenic activities, these metal(loid)s are bio-transferred into animal feed, which further results in the presence of metal(loid)s in milk due to bio transfer/accumulation. This systematic review collated information from published literature between 2000 and 2021. It focused on the global issue of metal(loid)s in milk, posing potential health risks. These contaminants enter the food chain through the bio-transfer/accumulation process from soil to animal feed to milk. The key metal(loid)s examined are arsenic (As), mercury (Hg), lead (Pb), and cadmium (Cd). A meta-analysis of 66 selected papers revealed the widespread presence of these contaminants in milk samples globally, with Pb being the most studied (43 %). This research estimated metal(loid)s levels or concentrations as 12.71 (95 % Confidence Interval (CI) = 0.16–25.26), 16.09 (95 % CI = 4.31–27.70), 197.04 (95 % CI = 75.28–318.18), 31.67 (95 % CI = 20.14–43.20) μg/kg (ppb) for As, Hg, Pb, and Cd, respectively using Stata™. The metal(loid) concentrations in milk were within the threshold limits other than Pb and Cd. Some studies in America, Africa, and Asia reported elevated Pb and Cd concentrations, raising health concerns. The simulated Risk Quotients (RQ) and Integrated Risk Quotient (IRQ) values generally remain above one, indicating potential human health risks. Notably, the IRQ value increases with more metal(loid)s consideration. Subgroup analysis indicates low-fat milk contains higher metal(loid)s concentrations. While metal(loid)s concentrations in milk largely comply with safety limits, some regions exhibit concerning concentrations. Therefore, continued surveillance to address potential health risks associated with metal(loid)s in milk is necessary to ensure dairy products' safety.

Evaluation of the realization of the genetic potential of cows of Jersey breed

An urgent issue in cattle breeding is the evaluation of the genetic potential of cattle. By monitoring the livestock, breeders have the opportunity to identify animals with various traits necessary in agriculture. Important traits include milk yield in cows and high levels of fat and protein content in milk. This type of animal evaluation helps to produce of young animals with increased milk productivity potential, which leads to increased profi ts in this industry. The main purpose of the research was to evaluate the implementation of the genetic potential of cows of Jersey breed housed at the enterprise LLC SKhP Novomarkovskoye in the Kantemirovsky district of the Voronezh region. During the research have been received results characterizing the milk productivity of the experimental cows. Thus, milk yield for the fi rst lactation was 6370 kg, and for the second it was 5780 kg, which was a high productivity indicator for cows of Jersey breed. The milk yield for 305 days of the fi rst lactation was 5765 kg, and in the second it was 5665 kg, which meets the international standard for assessing milk productivity. The mass fraction of fat in the milk under study was at the level of 5.63 %, which indicated a high fat content compared to other breeds of cows. Cows of Jersey breed are distinguished by the production of milk with a high fat content. The yield of milk fat in cows of Jersey breed was 324.5 kg. Milk with a high fat content usually contains more vitamins, such as A, D and E, they are fat-soluble and are better absorbed if consumed together with dairy products with a high fat content. It can be concluded on base of the data obtained that the choice of breeding animals with high genetic potential is important. At the same time, a properly selected and balanced ration for cows and housing animals under favorable conditions ensures the maximum realization of their genetic potential.

A population pharmacokinetic model for sertraline in women during the perinatal period-A contribution from the ConcePTION project.

Sertraline is frequently prescribed for mental health conditions in both pregnant and breastfeeding women. According to the limited available data, only small amounts of sertraline are transferred into human milk, yet with a large amount of unexplained interindividual variability. This study aimed to develop a population pharmacokinetic (popPK) model to describe the pharmacokinetics of sertraline during the perinatal period and explain interindividual variability. Pregnant women treated with sertraline were enrolled in the multicenter prospective cohort SSRI-Breast Milk study. A popPK model for sertraline maternal plasma and breast milk concentrations was developed and allowed estimating the milk-to-plasma ratio (MPR). An additional fetal compartment allowed cord blood concentrations to be described. Several covariates were tested for significance. Ultimately, model-based simulations allowed infant drug exposure through placenta and breast milk under various conditions to be predicted. Thirty-eight women treated with sertraline were included in the study and provided 89 maternal plasma, 29 cord blood and 107 breast milk samples. Sertraline clearance was reduced by 42% in CYP2C19 poor metabolizers compared to other phenotypes. Doubling milk fat content increased the MPR by 95%. Simulations suggested a median daily infant dosage of 6.9μg kg-1 after a 50 mg maternal daily dose, representing 0.95% of the weight-adjusted maternal dose. Median cord blood concentrations could range from 3.29 to 33.23 ng mL-1 after maternal daily doses between 25 and 150 mg. Infant exposure to sertraline, influenced by CYP2C19 phenotype and breast milk fat content, remains low, providing reassurance regarding the use of sertraline during pregnancy and breastfeeding.

Prevalence and concentration of aflatoxin M1 in breast milk in Africa: a meta-analysis and implication for the interface of agriculture and health

Breast milk is one of the many distinct forms of food that can be contaminated with aflatoxin M1 (AFM1). They may be consumed by eating contaminated foods, such as contaminated meat and crops, which would then be present in breast milk and cause health problems, including nervous system disorders and cancers of the lungs, liver, kidneys, and urinary tract. However, the prevalently inconsistent explanation of prevalence and concentration remains a big challenge. Thus, this meta-analysis was conducted to determine the prevalence and concentration of harmful chemicals in breast milk in an African context. The databases MEDLINE, PubMed, Embase, SCOPUS, Web of Science, and Google Scholar were searched for both published and unpublished research. To conduct the analysis, the collected data were exported to Stata version 18. The results were shown using a forest plot and a prevalence with a 95% confidence interval (CI) using the random-effects model. The Cochrane chi-square (I2) statistics were used to measure the studies' heterogeneity, and Egger's intercept was used to measure publication bias. This review included twenty-eight studies with 4016 breast milk samples and newborns. The analysis showed the overall prevalence and concentration of aflatoxin M1 in breast milk were 53% (95% CI 40, 65; i2 = 98.26%; P = 0.001). The pooled mean aflatoxin M1 concentration in breast milk was 93.02 ng/l. According to this study, the eastern region of Africa was 62% (95% CI 39–82) profoundly affected as compared to other regions of the continent. In subgroup analysis by publication year, the highest level of exposure to aflatoxins (68%; 95% CI 47–85) was observed among studies published from 2010 to 2019. This finding confirmed that more than half of lactating women’s breast milk was contaminated with aflatoxin M1 in Africa. The pooled mean aflatoxin M1 concentration in breast milk was 93.02 ng/l. According to this study, the eastern region of Africa was profoundly affected compared with other regions. Thus, the government and all stakeholders must instigate policies that mitigate the toxicity of aflatoxins in lactating women, fetuses, and newborns.

The Assessment of Iodine Concentrations in Colostrum and Breast Milk Using ICP-MS: The Impact of Delivery Type, Thyroid Function and Gestational Diabetes-A Pilot Study.

Considering the spectrum of benefits of breast milk feeding, determining the essential components of an infant's only food-mother's milk-seems justified, especially in the case of those whose deficiency (e.g., iodine) may result in developmental disorders. The main aim of this study was the determination of the total iodine content of breast milk (including colostrum and mature milk). A secondary objective was to assess the influence of factors such as the type of delivery, hypothyroidism, gestational diabetes or the stage of lactation on this parameter. The study materials were colostrum and milk after 1 (n = 14), 2 and 3 months (n = 8) of lactation with a range of iodine concentrations (µg/L): 195-1648 and 170-842, 174-650 and 273-751, respectively. Iodine was determined using the inductively coupled plasma mass spectrometry (ICP-MS). Multivariate statistical analysis revealed, e.g., that delivery by caesarean section or dose of L-thyroxine taken by women to normalise thyroid hormones, had a significant effect on iodine concentrations in breast milk. Further research aimed at assessing the quality of breast milk should also include determining the factors influencing it.

Human milk macro- and trace-elements: Simultaneous analysis in sub-milliliter amounts by ICP-MS and application to assessing acute supplementation effects

Adequate concentrations of human milk (HM) nutrients, including macro- and trace-elements, are essential for healthy growth and development of exclusively breastfed (EBF) infants. To monitor potential risk of deficiencies, and evaluate the effects of interventions like supplementation, accurate analysis is crucial. Even recent methods reporting on HM macro- and/or trace-elements describe multiple methodological approaches and the need for several milliliters. We optimized and validated a comprehensive method for simultaneous analysis of 13 macro- and trace-elements for simultaneous analysis by inductively-coupled plasma-mass spectrometry. 100–600 μL HM were microwave digested with ≤1.5 mL HNO3 (70 %). The digest was diluted to 5 % final acid concentration. He-Kinetic Energy Discrimination (KED; Na, K, P, Ca, Mg, Fe, Cu, Zn, Cr, Mo) and O2-Dynamic Reaction Cell (DRC; As, Mn, Se) modes minimized remaining interferences. Accuracy (NIST SRM 1869 infant formula; n = 15, 4 weeks) varied from 93.2 to 103 % (CV: 2.8–8.5 %) with trueness ranging from 93.9 to 104 %. Inter-day variation of a HM-pool (n = 20, 3 weeks) varied between 4.1 and 8.5 % for most elements; Cr, Mo, Mn (all<5 μg L−1) had higher variation, up to 25 %. Analyzing HM from 18 Bangladeshi mothers (2–4 months postpartum; day 1 = baseline, n = 17; day 2/3 = supplementation, n = 21 each) revealed higher concentrations for P, Ca, and Zn post-supplementation (p < 0.05, Friedman's Chi-Square Test). Na, Mg, Zn, and Se had the highest number of samples (>80 %) with concentrations below the Adequate Intake. Our method allows for simultaneous and reproducible analysis of macro- and trace-elements with concentrations ranging over 6 orders of magnitude, without the need for separate analytics and sample preparations, and requiring only sub-milliliter amounts of HM. Additional elements may be included after optimization and validation. The results from Bangladeshi HM samples indicate selective supplementation effects and concerningly low concentrations for some elements, which could adversely affect the EBF infant.

Selenium concentrations in expressed human milk: a systematic review and meta-analysis

BackgroundWe aimed to systematically review articles that reported selenium (Se) concentrations in human milk (HM).Methodsusing PRISMA guidelines we searched MEDLINE and Embase using the terms “human milk and Selenium”. All clinical trials and systematic reviews were retrieved.ResultsOut of 1141 articles, we reviewed 76 full-text articles, excluding 26. Thus, 50 articles were included in meta-analyses. Most studies (N = 31) did not specify whether milk was from mothers of preterm (PT) or fullterm (T) infants. In the other 19 studies, 1 examined PT-EHM, 15 T-EHM, and 3 both PT-EHM and T-EHM. In most studies, Se concentrations were highest in colostrum or until 3 months of lactation, then declined. Metaanalyzed means of PT-EHM or T-EHM provide estimates of Se intake a little higher than those reported to date, but still lower than current recommendations of intake.ConclusionThis study provides clinicians with useful estimates of Se intake in breast-fed infants.

Urea in the Diets of Dairy Cows

In most farms of the Nakhchivan Autonomous Republic, the basis of the feed balance of cattle consists of roughage, straw, hay, relatively less succulent feed and a very small part - fresh grass and fortified feed. That is why animals on these farms in winter stall conditions consume less digestible protein by an average of 40-55 g or more per unit of feed. On farms, urea was used to provide sufficient protein for cattle. During the experiment, cows of both groups (experimental and control) were fed in stall conditions and received the same number of feed shares according to their nutritional value. The only difference was that each cow in the experimental group was given an additional 80 g of urea per day. The results of our experience show that the daily milk yield of each cow that received 80 g of urea in the feed ration is on average 1,15 kg or 29,9% more compared to cows that did not receive urea, and the fat content of milk is from 0.1 up to 0,39%. The live weight of each cow receiving urea (over 123 days of experiment) was on average 17,47 kg greater than that of cows not receiving urea. The use of urea in livestock farming not only increases milk and meat productivity, but also reduces the cost of production.

Acceptance Analysis and Nutritional Content of Kluwih Seed Milk in Fulfilling Elderly Nutrition Intake

Kluwih seeds (Artocarpus communis) are a local food source that is still not widely used as a ready-to-eat food preparation. Kluwih seeds contain nutritional components such as protein, carbohydrates, fat, minerals and vitamins so that they can be used as a source of nutrients. The elderly are a group of people aged 60 years or over who currently need attention because the biological, physical and psychological aging that occurs in the elderly is feared to trigger inadequate food intake for the elderly so that adequate nutritional sources are needed. Kluwih seeds are a new inspiration to be processed into milk as a ready-to-eat food to meet the nutritional needs of the elderly. The aim of this research is to determine the acceptance of elderly people for kluwih seed milk and the percentage of nutritional content contained in kluwih seed milk. The research method used in this research is experimental. The samples in this study consisted of kluwih seed milk (P1), UHT milk (P2) and soy milk (P3). Acceptability analysis was carried out using scoring and hedonic test methods for taste, color and aroma parameters. Nutritional content analysis was carried out on the carbohydrate, protein and fat components of kluwih seed milk. Information on the acceptability of the elderly to kluwih seed milk was obtained from research subjects, namely 50 elderly panelists in 3 sample areas, namely Banjar Delod Padonan and Banjar Pengembungan, Pererenan Village and Banjar Negara, Sading Village, Mengwi District, Badung Regency, Bali. Based on the results of the hedonic test, elderly panelists in the sample area preferred kluwih seed milk (P1) over UHT milk (P2) and soy milk (P3), with the preferred characteristics of kluwih seed milk being yellowish white in color, very distinctive aroma, delicious and savory taste. The results of the nutritional content test show that kluwih seed milk contains 55.95% carbohydrates, 13.61% protein and 7.77% fat with a better nutritional ratio than UHT milk and soy milk.

Animal and Farm Factors Affecting the Fatty Acid Profile and Amyloid A Concentration of Milk on Quebec Dairy Farms

Relationships between farm and animal factors and the fatty acid (FA) profile and milk amyloid A (MAA) content of milk were determined in 336 Holstein dairy cows on 24 Quebec farms using multiple regression. Cows with a somatic cell count (SCC) above 200,000, and farms feeding palm oil were excluded. Independent factors of the regression models included days in milk (DIM), parity, yield, fat and protein contents of milk, SCC, and the dietary contents of neutral detergent fibre (NDF) and crude fat (CFAT). Non- significant variables with P-values &gt;0.25 were stepwise removed. Models with high fits were those of total short chain FA, medium chain FA, odd- and branched-chain FA (OBCFA), and saturated long chain FA with R2 of 0.33, 0.36, 0.34, and 0.41, respectively. The fat and protein contents and yield of milk did not affect the milk FA profile. Higher NDF increased the milk fat proportions of short chain FA and OBCFA and decreased those of monounsaturated (MUFA) and polyunsaturated FA (PUFA). Higher CFAT reduced this proportion of short chain FA but increased those of MUFA and PUFA. Increasing DIM reduced this proportion of short chain FA and increased that of medium chain FA. Higher SCC increased MAA.