Flavonoid Content Research Articles

Physicochemical characterization and sensory enhancement of cold plasma treated black whole wheat flour

This study examined the effects of cold plasma (CP) treatment on the physicochemical properties and sensory quality of black whole wheat flour (BWWF). Various factors including nutrient composition, color, amino acids, aroma, particle size, microstructure, antioxidant activity, and water migration were analyzed before and after CP treatment. The findings revealed that CP treatment had a minimal impact on the baseline nutrient composition of BWWF, but significantly improved its free amino acid profile, enhancing its nutritional value. The treated BWWF had an improved surface color, appeared brighter and yellower, and gave off a pleasant mellow aroma, while removing unpleasant flavor. The total phenolic content of BWWF increased while the flavonoid and anthocyanin content decreased after the treatment. CP-treated BWWF underwent aggregation cross-linking in the microstructure, and the content of bound water decreased, but the stability increased. In conclusion, CP treatment had great potential to improve the physicochemical properties and sensory quality of BWWF.

Integrated Analysis of Transcriptome and Metabolome Provides Insights into Flavonoid Biosynthesis of Blueberry Leaves in Response to Drought Stress.

Blueberries (Vaccinium spp.) are extremely sensitive to drought stress. Flavonoids are crucial secondary metabolites that possess the ability to withstand drought stress. Therefore, improving the drought resistance of blueberries by increasing the flavonoid content is crucial for the development of the blueberry industry. To explore the underlying molecular mechanism of blueberry in adaptation to drought stress, we performed an integrated analysis of the metabolome and transcriptome of blueberry leaves under drought stress. We found that the most enriched drought-responsive genes are mainly involved in flavonoid biosynthesis and plant hormone signal transduction pathways based on transcriptome data and the main drought-responsive metabolites come from the flavonoid class based on metabolome data. The UDP-glucose flavonoid 3-O-glucosyl transferase (UFGT), flavonol synthase (FLS), and anthocyanidin reductase (ANR-2) genes may be the key genes for the accumulation of anthocyanins, flavonols, and flavans in response to drought stress in blueberry leaves, respectively. Delphinidin 3-glucoside and delphinidin-3-O-glucoside chloride may be the most important drought-responsive flavonoid metabolites. VcMYB1, VcMYBPA1, MYBPA1.2, and MYBPA2.1 might be responsible for drought-induced flavonoid biosynthesis and VcMYB14, MYB14, MYB102, and MYB108 may be responsible for blueberry leaf drought tolerance. ABA responsive elements binding factor (ABF) genes, MYB genes, bHLH genes, and flavonoid biosynthetic genes might form a regulatory network to regulate drought-induced accumulation of flavonoid metabolites in blueberry leaves. Our study provides a useful reference for breeding drought-resistant blueberry varieties.

NaCl Stress Stimulates Phenolics Biosynthesis and Antioxidant System Enhancement of Quinoa Germinated after Magnetic Field Pretreatment.

Our previous study showed that magnetic field pretreatment promoted germination and phenolic enrichment in quinoa. In this study, we further investigated the effects of NaCl stress on the growth and phenolic synthesis of germinated quinoa after magnetic field pretreatment (MGQ). The results showed that NaCl stress inhibited the growth of MGQ, reduced the moisture content and weight of a single plant, but increased the fresh/dry weight. The higher the NaCl concentration, the more obvious the inhibition effect. In addition, NaCl stress inhibited the hydrolysis of MGQ starch, protein, and fat but increased the ash content. Moreover, lower concentrations (50 and 100 mM) of NaCl stress increased the content of MGQ flavonoids and other phenolic compounds. This was due to the fact that NaCl stress further increased the enzyme activities of PAL, C4H, 4CL, CHS, CHI, and CHR and up-regulated the gene expression of the above enzymes. NaCl stress at 50 and 100 mM increased the DPPH and ABTS scavenging capacity of MGQ and increased the activities of antioxidant enzymes, including SOD, POD, CAT, APX, and GSH-Px, further enhancing the antioxidant system. Furthermore, principal component analysis showed that NaCl stress at 100 mM had the greatest combined effect on MGQ. Taken together, NaCl stress inhibited the growth of MGQ, but appropriate concentrations of NaCl stress, especially 100 mM, helped to further increase the phenolic content of MGQ and enhance its antioxidant system.

Phytochemical and morpho-physiological response of Melissa officinalis L. to different NH4+ to NO3̄ ratios under hydroponic cultivation

BackgroundThe utilization of nutrition management, has recently been developed as a means of improving the growth and production of phytochemical compounds in herbs. The present study aimed to improve the growth, physiological, and phytochemical characteristics of lemon balm (Melissa officinalis L.) using different NH4+ (ammonium) to NO3̄ (nitrate) ratios (0:100, 25:75, 50:50, 75:25 and 100:0) under floating culture system (FCS).ResultsThe treatment containing 0:100 - NH4+:NO3̄ ratio showed the most remarkable values for the growth and morpho-physiological characteristics of M. officinalis. The results demonstrated that maximum biomass (105.57 g) earned by using the ratio of 0:100 and minimum at 75:25 ratio of NH4+: NO3̄. The plants treated with high nitrate ratio (0:100 - NH4+:NO3̄) showed the greatest concentration of total phenolics (60.40 mg GAE/g DW), chlorophyll a (31.32 mg/100 g DW), flavonoids (12.97 mg QUE/g DW), and carotenoids (83.06 mg/100 g DW). Using the 75:25 - NH4+:NO3̄ ratio caused the highest dry matter (DM), N and K macronutrients in the leaves. The highest antioxidant activity by both DPPH (37.39 µg AAE/mL) and FRAP (69.55 mM Fe++/g DW) methods was obtained in 75:25 - NH4+:NO3̄ treatment. The p-coumaric acid as a main abundant phenolic composition, was detected by HPLC analysis as the highest content in samples grown under 0:100 - NH4+:NO3̄ treatment. Also, the major compounds in M. officinalis essential oil were identified as geranial, neral, geranyl acetate and geraniol by GC analysis. With increasing NO3̄ application, geraniol and geranyl acetate contents were decreased.ConclusionsThe findings of present study suggest that the management of NH4+ to NO3̄ ratios in nutrient solutions could contribute to improving growth, physiological and phytochemical properties of M. officinalis. The plants treated with high nitrate ratio (especially 0:100 - NH4+:NO3̄) showed the greatest effects on improving the growth and production of morpho-physiological and phytochemical compounds. By comprehensively understanding the intricate dynamics among nitrogen sources, plants, and their surroundings, researchers and practitioners can devise inventive approaches to optimize nitrogen management practices and foster sustainable agricultural frameworks.

Bioactive phytocompounds profiling and the evaluation of analgesic, anti-inflammatory, and antihyperglycemic potential of Argyreia capitiformis (Poir.) Ooststr.: A combined in vitro, in vivo, and computational investigations

Ethnopharmacological relevanceArgyreia capitiformis (Poir.) Ooststr. (Convolvulaceae) is traditionally used by the Chakma community in the hilly region of Bangladesh to treat minor disorders such as pain. Aim of the studyThis study intended to determine the secondary metabolites to identify bioactive compounds and evaluate antioxidant potential, in vitro anti-inflammatory and in vivo analgesic, anti-inflammatory, and antihyperglycemic activities of A. capitiformis along with in silico investigations. Materials and methodsChemical profiling was carried out using HPLC and GC-MS analysis. The analgesic effect was measured employing tail immersion and acetic acid-induced writhing methods. Following protein denaturation and formalin-induced paw edema, anti-inflammatory activity was studied. The antihyperglycemic potential was assessed using an oral glucose tolerance test (OGTT), while further mechanistic investigation was conducted using an alpha-glucosidase enzyme inhibitory assay. Simulations and molecular docking analyses were performed to ascertain the stability and binding affinities of the drug-protein complex. ResultsA. capitiformis ethanolic extract confirmed the presence of phenolics, alkaloids, flavonoids, terpenoids, tannins, gums, steroids, and reducing sugars. HPLC analysis revealed the presence of eight polyphenolic compounds, the most abundant of which was myricetin (64.10 ± 0.14 mg per 100 g dry extract). Moreover, the GC-MS analysis revealed twenty-four molecules, the most important of which was 2,4-bis (dimethylbenzyl)-6-t-butylphenol (9.19%). The concentrations of total flavonoids, total terpenoids, total phenolics, and total tannins were ascertained to be 142.48 mg QE/g, 173.1 mg UAE/g, 19.35 mg GAE/g, and 13.05 mg GAE/g, respectively. Furthermore, the plant extract had a total antioxidant capacity of 388 mg AAE/g. In the writhing assay, the plant extract suppressed writhing by 59.73% and 76.99% at the doses of 250 and 500 mg/kg, respectively, compared to the standard diclofenac Na 87.17%, and in the tail immersion assay, the plant extract displayed a maximum average reaction time of 1.94 and 2.40 s at the doses of 250 and 500 mg/kg, respectively as compared to the control tramadol 2.84 s at 60 min. In an in vitro anti-inflammatory assay, the plant extract possessed an IC50 of 95.51 μg/ml while diclofenac Na (standard drug) was found to be 69.50 μg/ml. Afterward, in vivo anti-inflammatory activity was observed in mice over a period, particularly after 3 h, the plant extract exerted maximum percent inflammation inhibitions of 36.36% and 45.45% at the doses of 250 and 500 mg/kg, respectively whereas ibuprofen the standard drug (100 mg/kg) exhibited 61.82%. The plant extract demonstrated antihyperglycemic activity, lowering blood sugar levels to 5.7 and 4.62 mM at doses of 250 and 500 mg/kg, respectively, as opposed to 8.58 mM in the control group. Meanwhile, the standard drug glibenclamide (5 mg/kg) dropped blood glucose levels to 2.38 mM in 60 min after glucose administration. Molecular docking (MD) and molecular dynamics simulation (MDS) studies support the stability of the protein complex responsible for exerting pharmacological activities. ConclusionA. capitiformis extract exhibited strong medicinal values supporting its traditional uses.

From parasitic life to health-promoting applications - A versatile goldmine discovered in nature's secret treasure chest: Orobanche nana

Studies evaluating the phytoconstituents and therapeutic potential of species belonging to Orobanche (Family Orobanchaceae) are rather limited. The present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of the aerial parts of Orobanche nana Noë ex Rchb. Extracts were prepared by sequential maceration in dichloromethane, ethyl acetate and ethanol and finally an aqueous extraction by infusion were performed. NMR and LC-DAD-MS analysis allowed the identification of different phytoconstituents including the characteristic compounds of Orobanche like verbascoside, poliumoside, orobanone and orobanchoside. The ethanol extract displayed the highest total phenolic (115.63 mg GAE/g) and flavonoids (16.53 mg RE/g) contents, antiradical (DPPH = 623.70 mg TE/g; ATBS = 843.50 mg TE/g) and ions reducing (Cu++ = 725.39; Fe+++ = 617.70) properties while the aqueous extract exerted the best chelating power (19.54 mg EDTAE/g). It was determined that EtOH and Water extracts applied to HepG2 cells decreased the levels of anti-apoptotic proteins p-NFκB, BCL-2 and BCL-XL, while increasing the levels of apoptotic proteins BAX and p-53, thus inhibiting cell survival. In addition, Orobanche nana played an active role in the rearrangement of overexpress MMPs that cause disruption of ECM homeostasis. We employed Network Pharmacology to investigate the mechanisms through which Orobanche nana's compounds impact Kidney and Liver diseases. These findings suggested that O. nana could be a promising source of bioactive molecules with potential therapeutic applications.

The phytochemical composition of Kuzbass medicinal plants studied by spectrophotometry and chromatography

Flavonoids are plant polyphenols that exhibit biological activity with antibacterial, antiviral, antioxidant, anti-inflammatory, antimutagenic, and anticarcinogenic effects. The medicinal plants of Kuzbass have high contents of flavonoids and other polyphenolic compounds. Therefore, they can be used in medicinal preparations to prevent or treat serious diseases. We studied the following plants collected in Kuzbass: common thyme (Thymus vulgaris Linn., leaves and stems), woolly burdock (Arctium tomentosum Mill., roots), alfalfa (Medicago sativa L., leaves and stems), common lungwort (Pulmonaria officinalis L., leaves and stems), common yarrow (Achillea millefolium L., leaves and stems), red clover (Trifolium pratense L., leaves and stems), common ginseng (Panax ginseng, roots), sweetvetch (Hedysarum neglectum Ledeb., roots), and cow parsnip (Heracleum sibiricum L., inflorescences, leaves, and stems). To extract flavonoids, we used ethanol at concentrations of 40, 55, 60, 70, and 75%. Spectrophotometry was used to determine total flavonoids, while high-performance liquid chromatography was employed to study the qualitative and quantitative composition of the extracts. The highest yield of flavonoids was found in H. sibiricum leaves (at all concentrations except 70%), followed by the 55% and 70% ethanol extracts of T. vulgaris leaves and stems, as well as the 75% ethanol extract of A. millefolium leaves and stems. Thus, these plants have the greatest potential in being used in medicines. High-performance liquid chromatography showed the highest contents of polyphenolic compounds in the samples of P. officinalis, A. millefolium, T. vulgaris, and T. pratense. Our results can be used in further research to produce new medicinal preparations based on the medicinal plants of Kuzbass.

Controle de qualidade da droga vegetal Calendula officinalis L. disponível na cidade de Ponta Grossa (PR)

Introduction: Calendula officinalis L. is a herbaceous plant belonging to the Asteraceae family that contains flowers as parts used to obtain analgesic, anti-inflammatory, antiseptic, healing, and dermoprotective effects. Objective: The objective of the present work was to analyze and characterize samples of C. officinalis, sold in Ponta Grossa (PR), and verify their compliance with current legislation, presenting the quality control provided for by the Brazilian Pharmacopoeia (2019). Method: After selecting the selections, tests were carried out on the quality control of the herbal drug by checking the packaging and labeling, macroscopic and microscopic identification, purity tests, analysis of loss due to desiccation and total ash, thermal analysis, profile of the aqueous extract in thin layer chromatography, and source of flavonoids from secondary metabolites. Results: All samples were identified as C. officinalis and were approved in the chromatographic profile and flavonoid content; however, sample B did not meet the pharmacopoeia purity requirements. Conclusion: Medicinal plants with quality deviations may suffer a significant reduction in their therapeutic effect, in addition to generating possible side and toxic effects.

In vitro Anti-oxidant, Alpha-Amylase, and Alpha-Glucosidase Inhibition Studies of Physica aipolia (Ehrh.ex Humb.) Furnr

Background: Diabetes prevalence is progressively rising everywhere, particularly in developing countries. Lichens have evolved into a rich source of innovative bioactive chemicals with their anti-oxidant capabilities, widening the scope of well-documented and effective diabetes treatment. Objective: The main objectives of this study were to perform in vitro alpha-amylase, alphaglucosidase inhibition analysis, and anti-oxidant of lichen Physica aipolia. Method: Folin Ciocalteu’s reagent was used to determine the total phenolic content for biological activities. The Aluminum trichloride method was used to determine total flavonoid content, and the free radical assay method was used to determine the antioxidant activity of P. aipolia using 2,2- diphenyl-1-picrylhydrazyl (DPPH). Moreover, substrate 2-Chloro-4-nitrophenyl-α-D-maltotrioside (CNPG3) and substrate-nitrophenyl-α-D-glucopyranose (p-NPG) were used for the determination of alpha-amylase and alpha-glucosidase inhibition activities respectively, and molecular docking was performed by Auto Dock Vina. Results: Total phenolic and flavonoid contents present in P. aipolia were 37.41 ± 2.87 mg GAE/g and 5.53 ± 0.95 mg QE/g, respectively. For anti-oxidant inhibition activity, crude extract of methanol showed an IC50 value of 15.324 ± 0.80 μg/mL. Furthermore, a methanolic crude extract showed significant inhibition activities against alpha-amylase (IC50 = 178.50 ± 1.10 μg/mL) and alphaglucosidase (IC50 = 76.10 ± 0.91 μg/mL). During in silico analysis, zeorin showed an effective binding affinity with -7.9 kcal/mol, and Atranorin showed -7.4 kcal/mol in the orthosteric site of the protein. Conclusion: Physica aipolia exhibits the potential to impact biological functions, demonstrating antidiabetic properties, as confirmed by molecular docking analysis in silico.

Phytochemicals, antioxidant activity and nutritional profile of pulp, peel and peel fiber of mango (<i>Mangifera indica</i> l.) cultivar Ataulfo

Introduction: Mango fruit and its by-products represent a very diverse and abundant source of sugars and components with anti-inflammatory and antioxidant properties, such as dietary fiber, vitamins, and polyphenols. Consuming mango pulp might reduce health disorders caused by inflammatory and oxidative processes such as obesity related diseases. Mango and its by-products contain compounds that can either promote or neutralize inflammatory and oxidative environments; variations in their composition may directly influence the degree of bioactivity. This work aimed to analyze the nutritional and nutraceutical profiles presented in the three different mango by-products to know their possible nutraceutical potential. Objective: This work aimed to analyze the nutritional and nutraceutical profiles presented in the three different mango by-products to know their possible nutraceutical potential. Methods: Ripe Ataulfo mango products (pulp, peel, and peel fiber) were evaluated for nutritional composition by AOAC methods. β-carotene and ascorbic acid were analyzed by UV-Vis liquid chromatography (LC), while mangiferin, phenolics and flavonoids by LC coupled to mass spectrometer detector. The antioxidant capacity was correlated to each of the bioactive compounds. Results: Ripe Ataulfo mango products (pulp, peel, and peel fiber) were evaluated for nutritional, antioxidant, and phytochemical characteristics. All the by-products demonstrated significant differences (P ≤ 0.05) in nutritional and chemical composition. Mango peel had the highest β-carotene, vitamin C, and mangiferin values. Mango pulp had the highest values for free sugars (73 g 100 g-1), but low phytochemicals values, contrasting the peel fiber with 81% of total dietary fiber but minimum values of free sugars and β-carotene and no vitamin C. The highest antioxidant capacity was presented for mango peel. Results obtained from this study indicate that the mango Ataulfo pulp and by-products are divergent and represent a good source of bioactive compounds. Conclusions: Mango peel composition is a natural combination of high concentrations of mangiferin, flavonoids, phenolic acids, vitamin C, provitamin A, and dietary fiber, all involved with inflammation and cell oxidative stress-related disease control and prevention. However, mango peel exhibited the most significant nutraceutical potential due to its high antioxidant capacity. Keywords: Mangifera indica L.; Mango Ataulfo; Mangiferin; Flavonoids; Phytochemicals; Bioactive Compounds

A pharmacological investigation for therapeutic potential of Callistemon citrinus as an anthelmintic agent (Bottle-Brush Plant)

The growing resistance of parasitic helminths to standard anthelmintic medications has sparked increased interest in exploring alternative treatments, particularly those derived from herbal sources. This research focuses on the anthelmintic properties of selected herbal plants, assessing their ability to inhibit and eliminate parasitic worms. Standard extraction techniques were employed to prepare various plant extracts, which were then tested using both in vitro and in vivo assays to determine their effectiveness. The findings revealed that several plant extracts showed significant anthelmintic effects, comparable to those of conventional medications. Phytochemical analysis identified a high concentration of alkaloids, tannins, saponins, and flavonoids, which are thought to be the primary contributors to the observed activity. These results indicate that these herbal plants may serve as promising natural anthelmintic agents, providing a sustainable and environmentally friendly alternative to synthetic drugs. Further research is necessary to isolate and identify the specific bioactive compounds, clarify their mechanisms of action, and assess their safety and efficacy in clinical applications. This study highlights the potential of herbal medicine in developing novel anthelmintic treatments and encourages the integration of traditional knowledge with modern scientific research.

Sumac extract as a natural preservative in mayonnaise: Effects on lipid oxidation, microbial growth, physicochemical, rheological, and sensory characteristics

The present study was conducted to evaluate the effect of sumac extract (SE) as a natural antioxidant and antimicrobial agent on the quality characteristics of low-fat mayonnaise during 63 days of storage. The SE was used at levels of 1 % (SE1%), 3 % (SE3%), and 5 % (SE5%) in the formulations. The two control treatments were produced with chemical preservatives (C1) and without preservatives (C2). The SE caused a significant decrease in peroxide and anisidine values compared to controls after 63 days (4.73–5.77 meq/kg vs. 6.33–6.73 meq/kg for peroxide values, and 6.29–11.21 vs. 12–13.60 for anisidine values, P < 0.05). The higher inhibitory effects of SE on microbial growth in mayonnaises were revealed after 63 days, where the total viable count declined from the highest value (36.5 log CFU/g) in C2 to the lowest count (12 log CFU/g) in SE5%. The yeasts and molds count in SE1% (4.5 log CFU/g) were lower than the counts of 20.5 log CFU/g and 23.5 log CFU/g observed in C1 and C2, respectively. Moreover, significant reductions in Lactic Acid Bacteria were observed in SE-enriched treatments (P < 0.05). The phenolic and flavonoid contents of mayonnaise treatments besides antioxidant capacity were enhanced by increasing SE concentrations. However, the lowest storage modulus and complex viscosity were observed in SE5%. The highest overall acceptance belonged to C1 followed by SE1% at the end of storage. Finally, SE has the potential to be used as a natural preservative in mayonnaise.

Comprehensive Analysis of Sulfated Flavonoids in Eclipta prostrata for Quality Evaluation

Eclipta prostrata (Asteraceae) is employed as a hemostatic agent in many traditional medicines, owing to its sulfated flavonoid content. In this study, we obtained crude drug samples from three provinces collected in different years and analyzed their sulfated flavonoid contents using liquid chromatography–mass spectrometry (LC–MS) for quality evaluation. Because sulfated flavonoids are unstable and difficult to isolate from extracts, this study first synthesized a variety of sulfated flavonoids and accumulated spectral data in order to identify the compounds in E. prostrata. The LC–MS analysis of six crude drug samples revealed the presence of luteolin 7-sulfate, apigenin 7-sulfate, diosmetin 7-sulfate, and diosmetin 3′-sulfate. The samples without luteolin 3′-sulfate featured high apigenin 7-sulfate content. Although the samples were collected from the same locality, their compositions differed depending on the year of collection. Further, they were classified according to three patterns: (1) samples with luteolin 7-sulfate as the main component, (2) samples with apigenin 7-sulfate as the main component, and (3) samples with relatively high diosmetin sulfate content. Luteolin 7-sulfate typically exhibits relatively high erythrocyte aggregation efficiency and fibrinogen aggregation rate. These results demonstrate that the analysis of sulfated flavonoids is beneficial for the quality evaluation of E. prostrata for hemostatic applications.

The impact of genetic similarity and environment on the flavonoids variation pattern of Cyclocarya paliurus

The leaves of Cyclocarya paliurus (Batalin) Iljinskaja, an endemic tree with a scattered distribution in subtropical China, are rich in flavonoids with beneficial, health-promoting properties. To understand the impact of environment and genetic similarity on the variation pattern of flavonoids in this species, we analyzed C. paliurus germplasm resources from 26 different populations previously sampled from the main distribution area. Environmental, genetic and biochemical data was associated by genetic structure analysis, non-parametric tests, correlation analysis and principal component analysis. We found that populations with higher flavonoid contents were distributed at higher elevations and latitudes and fell into two groups with similar genetic diversities. Significant accumulations of isoquercitrin and kaempferol 3-O-glucoside were detected in the higher flavonoid-content resources. In addition, the genetic clusters with higher flavonoid contents exhibited broader environmental-adaptive capacities. Even in the presence of environmental factors promoting C. paliurus flavonoid accumulation, only those populations having a specific level of genetic similarity were able to exploit such environments.

Innovative Approaches to Camembert Cheese: Optimizing Prebiotics and Coagulation Conditions for Enhanced Quality and Nutrition

The objective of this study is to investigate how different factors, such as lactic acid bacteria, prebiotics (flaxseed powder, watercress seed powder, okra mucilage), and coagulation temperature influence the final quality of curd by conducting three optimization experiments and implementing a structured experimental plan. In the first phase, milk coagulation was assessed at 45 °C with various combinations of lactic acid bacteria (probiotics) and prebiotics (powdered flaxseed and watercress). In the second investigation phase, the effects of lowered probiotic and prebiotic (powdered flaxseed and watercress) concentrations were examined at the coagulation temperature of 38 °C. We investigated the concentration of lactic acid bacteria at 3 mg/mL of milk and the effects of temperature and prebiotics (okra mucilage and flaxseed powder). We observed short milk clotting time (2 s) using the optimized mixture (0.18 mg of probiotics, 1.5 mg of flaxseed powder, and 1.147 mg of watercress powder) per 10 mL of milk. It contrasts with the classical coagulation way optimized at (5.9 and 9.5 s), which were generated at optimal temperatures of 45 and 45.7 °C, respectively. Our new mixture improves the fermentation process of camembert cheese at 38 °C. This cheese had a high flavonoid content, fewer lactic bacteria and molds, a homogeneous texture, and no outer crust, and exceptional sensory attributes such as a creamy and fluid paste. These attributes suggest its potential benefits as a dairy product for individuals with cardiovascular and gastrointestinal conditions.

Epicatechin Isolated from Litchi chinensis Sonn. (Litchi) Fruit Peel Ethyl Acetate Extract Modulated Glucose Uptake in Chang Cells and Suppressed ROS Production in RAW 264.7 Macrophages.

Bioactive flavonoid epicatechin has been reported in the peel of litchi fruit but isolated from its hydroalcoholic extracts. This study isolated epicatechin with cellular glucose uptake modulatory and ROS production inhibitory properties from the ethyl acetate (EtOAc) extract using a bioassay-guided approach. The fruit peel was defatted with hexane and sequentially extracted using dichloromethane (DCM), EtOAc, methanol (MeOH) and water. In vitro phytochemical models, namely antioxidant (Fe3+ reducing, radical scavenging and anti-linoleic acid peroxidative) and glycaemic control (α-glucosidase and α-amylase inhibitory and glucose uptake modulatory), were employed for the bioassay-guided isolation, while the isolated compound was characterised using NMR and mass spectrometry and assessed for dose-dependent inhibition of α-glucosidase and lipopolysaccharide (LPS)-induced cellular ROS production, as well as modulation of cellular glucose uptake. Relative to the other extracts, the EtOAc extract had appreciable phenol and flavonoid contents, which perhaps influenced its potent anti-lipid peroxidative (65.0%) and α-glucosidase inhibitory (52.4%) effects. The α-glucosidase inhibitory potency of the fractions (1-8) from the EtOAc extracts correlated with their flavonoid contents, with fraction 5 outperforming other fractions. The fraction comprised a pool of fractions obtained from the DCM:MeOH:water (7:3:0.281 v/v/v) solvent system. LC-MS revealed the predominant presence of epicatechin in fraction 5, which was later isolated from one of the sub-fractions (sub-fraction 4) of fraction 5. This sub-fraction had stronger anti-lipid peroxidative (65.5%), α-glucosidase inhibitory (65.8%) and glucose uptake modulatory (38.2%) effects than the other sub-fractions from fraction 5, which could have been influenced by the isolated epicatechin. Moreover, the isolated epicatechin inhibited α-glucosidase (IC50 = 35.3 µM), modulated cellular glucose uptake (EC50 = 78.5 µM) and inhibited LPS-induced ROS production in RAW 264.7 macrophages in a dose-dependent fashion [IC50 = 18.9 µM; statistically comparable (p > 0.05) to ascorbic acid, IC50 = 9.57 µM]. Epicatechin from litchi peel EtOAc extract could potentiate glucose uptake modulatory, α-glucosidase inhibitory and ROS suppressive capacities, which could be influential in the use of litchi fruit peel for managing diabetes and associated oxidative damage.

Phytochemical Profiling and In vitro Antioxidant Activity of Methanolic Extracts from Fadogia cienkowskii Leaves

This study assessed the phytochemicals in the methanolic extract of Fadogia cienkowskii leaves and ascertained their antioxidant qualities In vitro, taking into account the plant's extensive usage in traditional medicine. Standard procedures were followed in the qualitative and quantitative phytochemical examination as well as the In vitro antioxidant activities of the extracts. The methanolic extracts of F. cienkowskii leaves produced a yield of 4%. The main phytochemical components found in the plant were phenolics (tannins and flavonoids), saponins, protein, and carbohydrates. The amount of total phenols in the dried plant extract was 2.085± 0.35 μg garlic acid equivalent (GAE)/mg, tannins in the extract was 0.755±0.17 μg GAE/mg, flavonoid contents in the extract was 3.646± 0.45 μg GAE/mg, and flavonols in the extract was 0.868± 0.20μg quercetin equivalent/mg. At a dosage of 10,000μg/ml, the extract demonstrated a noteworthy dose-dependent ability to scavenge DPPH radicals, effectively blocking 96.56 ± 0.46 % of DPPH, in contrast to ascorbic acid, which inhibited 89.10 ± 3.73 % at the same concentration. At a concentration of 2500μg/ml of extract, the extract demonstrated the highest level of superoxide anion (O2) inhibitory action, at 71.91±0.66% of the radical, in comparison to quercetin, which exhibited 68.23 ± 0.41% at 250μg/ml. The extract's ability to scavenge Nitric Oxide (NO) was concentration-dependent, with 250µg/ml of it being more effective than α-tocopherol. These results consequently imply that the leaf extract of F. cienkowskii likely mediates its medicinal potentials through the efficient scavenging of free radicals. Therefore, the plant can be used to create safe and natural antioxidant compounds because it has the potential to scavenge free radicals and contains sufficient concentrations of phytochemicals.

Bellevalia pseudolongipes Plant: Comprehensive Analysis of the Elemental Composition and Total Phenolic and Flavonoid Contents

Bellevalia pseudolongipes is a recently described plant species, whose mineral and biochemical composition have not yet been reported. Thus, this study examined the mineral composition and total phenolic and flavonoid contents of the B. pseudolongipes plant. Elemental analysis revealed that the plant contained high levels of calcium (22379.556 ± 025 ppm) and potassium (19721.157 ± 005 ppm). Phenolic compound analysis demonstrated a high total phenolic content (0.24 ± 0.004 mg gallic acid equivalent/g sample), thus highlighting the antioxidant capacity of the plant. Additionally, the assessment of flavonoid content (0.043 ± 0.001 mg catechin equivalent/100 g sample) indicated the potential use of the B. pseudolongipes plant as a source of antioxidants. These findings underscore B. pseudolongipes as a valuable natural resource for human health because it is rich in antioxidants, minerals, and phenolic compounds, while also providing a crucial foundation for researchers interested in exploring its pharmacological, medical, and industrial potential. The results of this study contribute to our understanding of the biological and health values of plants, thereby providing support for the development of products that are derived from natural sources and innovative solutions that contribute to human health.

Centaurea dimorpha Viv. (Asteraceae) growing in Algeria extracts as a promising natural cosmetic active ingredient: Broad-spectrum photoprotection and antioxidant efficacy

Recently, plants have been considered as a valuable natural source of cosmetic active ingredientsowing to their sustainability and weak toxicity. Thus, this study was conducted to investigate the cosmetic efficacy of Centaurea dimorpha Viv (Asteraceae), an endemic species of North Africa, as a promising natural cosmetic active ingredient. In the present study, Ethyl acetate and Butanolic extracts obtained from powdered Centaurea dimorpha aerial parts were investigated for their phenolic and flavonoid contents, which were evaluatedvia Folin-ciocalteu reagent and aluminium chloride methods, the in vitro antioxidant potential was evaluated by DPPH radical scavenging,phosphomolybdenum and phenanthroline assays, the UVB and broad spectrum protective efficacy was spectrophotometrically assessed by measuring SPF, UVA/UVB ratio and critical wavelength (CW) indices. The highest levels of TPC and TFCwere recorded by the ethyl-acetate extract (119.20 ± 0.32 µg GAE mg-1, 50.65 ± 0.43 µg QEmg-1, respectively). Similarly, this extract displayed a significant antioxidant effect, particularly in the phenanthroline assay (152.63 ± 0.49 µg AAEmg-1). Ethyl acetate extract also showed UVB and broad-spectrum (UVB-UVA) protective efficacy (SPF=12.30 ± 0.001, UVA/UVB ratio=0.63 ± 0.001, λc= 371). The results obtained show the possibility to use Ethyl acetate extract as a promising active ingredient for sunscreen formulations.

Flavonoids from Verbascum thapsus Protect Against Nephrotoxicity in Rats

General Background: Nephrotoxicity induced by carbon tetrachloride (CCl4) poses significant health risks, prompting the exploration of natural antioxidants for renal protection. Specific Background: Verbascum thapsus, a plant known for its flavonoid content, has shown potential in mitigating oxidative stress, but its specific protective effects against CCl4-induced nephrotoxicity remain under-investigated. Knowledge Gap: While previous studies have indicated antioxidant properties of flavonoids, their efficacy in renal preservation in vivo has not been fully elucidated. Aims: The study evaluated the antioxidative and nephroprotective properties of flavonoids from Verbascum thapsus leaves in a rat model of CCl4-induced nephrotoxicity.Results: Rats were divided into three groups: control, CCl4-treated, and flavonoid plus CCl4-treated. Biochemical analyses revealed that CCl4 significantly elevated malondialdehyde (MDA), urea, creatinine, and uric acid levels while decreasing superoxide dismutase (SOD) and glutathione (GSH) levels. Notably, flavonoid administration markedly reduced MDA, urea, creatinine, and uric acid levels and enhanced SOD and GSH levels compared to the CCl4-only group. Novelty: This study uniquely highlights the protective role of flavonoids from Verbascum thapsus against nephrotoxicity, demonstrating both their antioxidative capacity and renal preservation in an experimental model. Implications: The findings support the potential use of Verbascum thapsus as a therapeutic agent in the management of kidney diseases, suggesting further investigation into its application in clinical settings to address nephrotoxicity and related renal disorders. Highlights: Nephroprotective: Flavonoids significantly protect against CCl4-induced kidney damage. Biochemical Enhancement: Improved antioxidant levels; reduced harmful biochemical indicators. Therapeutic Potential: Supports natural treatments for kidney diseases. Keywords: Verbascum thapsus, flavonoids, nephrotoxicity, antioxidants, carbon tetrachloride