Nowadays, an anaerobic filter is used in treating various wastewaters that is more effective for denitrification and organic removal. The Anaerobic Filter with Inoculum, Soft and Hard Packing Medium (AFISH) and Double-Filling Aerobic Baffle (DFAB) reactors were continuously operated for 120 days with a short Hydraulic Retention Time (HRT) in the range of 1 and 2 days at an Organic Loading Rate (OLR) of 0.58 and 1.08 gCOD/(l.d) by daily feeding the influent Chemical Oxygen Demand (COD) concentration between 1169 and 250 mg/l. After treating the sample wastewater in the new AFISH reactor, the effluent flowed into the DFAB reactor for further treatment to determine the treatment effectiveness of the novel AFISH and DFAB reactors at a short HRT of 1 to 2 days. The influent COD was reduced to 376 mg/l to 250 mg/l in a novel AFISH effluent and further dropped to 220 mg/l to 102 mg/l following DFAB reactor treatment. The COD removal in the AFISH reactor was around 67% when HRT was about 1 day, and a high COD value of 78% was removed during the HRT of 2 days, while in the DFAB reactor, the capability of treatment reached between 40% and 57% of the COD removed at the HRT of 1 and 2 days. The concentration of NH4-N in the effluent of the novel AFISH reactor was significantly lower than the effluent of the DFAB reactor, with NH4-N concentrations ranging from 157 mg/l to 330 mg/l. However, the treatment of vegetable bio-slurry from the Internal Circulation (IC) reactor by the novel AFISH and DFAB reactors satisfied the secondary wastewater discharge standard in the Integrated Wastewater Discharge Standard. Therefore, this novel process is one method beneficial for current and further research development that can be applied for industrial wastewater treatment processing and provides science information to those interested in research in the future.