High Fatty Acid Content Research Articles

Fatty acid content and profile of round sardinella (Sardinella aurita), an expanding thermophilic species in the NW Mediterranean

Over the past few decades, due to sea warming driven by global climate change, the habitat range of round sardinella (Sardinella aurita) has expanded into the north-western Mediterranean Sea. This study evaluates the fatty acid content and fatty acid profile of this small, warm-water pelagic fish caught in this area, specifically the northern Catalan coast. The findings provide important insights into the nutritional status of the species under changing environmental conditions. The study confirms that fatty acid content varies according to the individual specimen’s maturity stages (immature, pre-spawners, and spawners). Pre-spawners showed higher fat levels compared to spawners, whose lipids are allocated to their gonads to enhance the survival of their offspring. While a high content of omega-3 polyunsaturated fatty acids (n-3 PUFAs) was generally found at all stages of maturity, pre-spawners had higher levels of EPA but lower levels of DHA than immature individuals and spawners. Moreover, compared to small pelagic fish more typical of the temperate waters in the region (European anchovy, Engraulis encrasicolus, and European sardine, Sardina pilchardus), round sardinella generally have a higher level of PUFAs, especially DHA. Encouraging consumption of this warm water species could provide significant nutritional benefits while reducing fishing pressure on European sardine and European anchovy, whose populations are currently in a very poor state.

Cold Hydraulic Extraction Optimization and Characterization of Balanites aegyptiaca and Ceiba pentandra Seed Oils

Non-edible vegetable oils have a huge potential for many industrial applications. Most of them are expressed by mechanical pressing, but data on the different operating conditions to achieve optimal extraction yields and high-quality oil are scarce. Hence, the objective of this study was to optimize key hydraulic extraction parameters at ambient temperature (25 °C) of <em>Balanites aegyptiaca</em> and <em>Ceiba pentandra</em> oils by increasing the oil recovery. Optimal holding time, applied pressure, compression speed, and press cage charge were determined for whole and crushed oleaginous material. The results indicate that the <em>B.aegyptiaca </em>kernels and <em>C.pentandra</em> seeds contained 2.7 and 5.7% moisture, as well as 46.3 and 26.2% fat, respectively. Under the optimal operating conditions, the oil recovery (wt.%. d.b.) from whole (crushed) material was 73.4 (69.8) <em>B.aegyptiaca </em>kernels and 38.2 (41.2) <em>C.pentandra</em> seeds.<strong> </strong>The extracted oils have a high content of unsaturated fatty acids including of about 74 wt.% for <em>B. aegyptiaca</em> and 69 wt.% for <em>C.pentandra</em>. They were particularly rich in linoleic ω-6 acid, 45.32 and 38.77 wt.%, respectively. The chemical characteristics of cold-extracted <em>B. aegyptiaca</em> and <em>C.pentandra</em> oils were 10 and 20 ppm phosphorus, 2.8 and 3.5 ppm iron, 0.0 ppm copper, 0.06 and 0.1% water-volatile matter, respectively. These valuable material properties make the <em>B.aegyptiaca</em> and <em>C.pentandra</em> oils an ecological and renewable resource for multipurpose applications.

A contribution to lipid digestion of Odobenidae family: Computational analysis of gastric and pancreatic lipases from walrus (Odobenus rosmarus divergens)

Triacylglycerols (TAGs) are a primary energy source for marine mammals during lipid digestion. Walruses (Odobenus rosmarus divergens) consume prey with a high content of long-chain polyunsaturated fatty acids; however, their digestive physiology and lipid digestion remain poorly studied. The present study aims to model and characterize the gastric (PWGL) and pancreatic (PWPL) lipases of Pacific walruses using an in-silico approach. The confident 3D models of PWGL and PWPL were obtained via homology modeling and protein threading and displayed the structural features of lipases. Molecular docking analysis demonstrated substrate selectivity for long-chain TAG (Trieicosapentaenoin; TC20:5n-3) in PWGL and short-chain TAG (Trioctanoin; TC8:0) in PWPL. Molecular dynamics simulations demonstrate that PWGL bound to tridocosahexaenoin (TC22:6n-3), the protein is considerably stable at all three salinity conditions, but fluctuations are observed in the regions associated with catalytic sites and the lid, indicating the potential hydrolysis of the substrate. This is the first study to report on the digestion of TAGs in walruses, including modeling and lipases characterization and proposing a digestive tract for pinnipeds.

Roseiterribacter gracilis gen. nov., sp. nov., a novel filterable alphaproteobacterium isolated from soil using a gel-filled microwell array device.

Our previous studies indicate the abundant and diverse presence of yet-to-be-cultured microorganisms in the micropore-filtered fractions of various environmental samples. Here, we isolated a novel bacterium (designated as strain TMPK1T) from a 0.45-μm-filtered soil suspension by using a gel-filled microwell array device comprising 900 microwells and characterized its phylogenetic and physiological features. This strain showed low 16S rRNA gene sequence identities (<91%) and low average nucleotide identity values (<70%) to the closest validly described species, and belonged to a novel-family-level lineage within the order Rhodospirillales of Alphaproteobacteria. Strain TMPK1T exhibited small cell sizes (0.08-0.23 μm3) and had a high cyclopropane fatty acid content (>13%), and these characteristics were differentiated from other Rhodospirillales bacteria. A comprehensive habitability search using amplicon datasets suggested that TMPK1T and its close relatives are mainly distributed in soil and plant-associated environments. Based on these results, we propose that strain TMPK1T represents a novel genus and species named Roseiterribacter gracilis gen. nov., sp. nov. (JCM 34627T = KCTC 82790T). We also propose Roseiterribacteraceae fam. nov. to accommodate the genus Roseiterribacter.

In Vitro Predictive Model for Intestinal Lymphatic Uptake: Exploration of Additional Enhancers and Inhibitors.

Drug absorption via chylomicrons holds significant implications for both pharmacokinetics and pharmacodynamics. However, a mechanistic understanding of predicting in vivo intestinal lymphatic uptake remains largely unexplored. This study aimed to delve into the intestinal lymphatic uptake of drugs, investigating both enhancement and inhibition using various excipients through our previously established in vitro model. It also examined the applicability of the model by assessing the lymphatic uptake enhancement of a lymphotropic formulation with linoleoyl polyoxyl-6 glycerides using the same model. The model successfully differentiated among olive, sesame, and peanut oils in terms of lymphatic uptake. However, it did not distinguish between oils containing long-chain fatty acids and coconut oil. Coconut oil, known for its abundance of medium-chain fatty acids, outperformed other oils. This heightened uptake was attributed to the superior emulsification of this oil in artificial chylomicron media due to its high content of medium-chain fatty acids. Additionally, the enhanced uptake of the tested formulation with linoleoyl polyoxyl-6 glycerides underscored the practical applicability of this model in formulation optimization. Moreover, data suggested that increasing the zeta potential of Intralipid® using sodium lauryl sulfate (SLS) and decreasing it using (+/-) chloroquine led to enhanced and reduced uptake in the in vitro model, respectively. These findings indicate the potential influence of the zeta potential on intestinal lymphatic uptake in this model, though further research is needed to explore the possible translation of this mechanism in vivo.

Exploring the Potential of High-Fatty Acid Content Oils for Biodiesel Production: A Catalyst-Free Approach

Abstract The adoption of biofuels has been recognized as one of the key approaches to strive towards achieving net zero emissions, particularly through the utilization of biodiesel derived from oils or fatty acids. To further optimize the utilization of biodiesel, it becomes crucial to explore alternative non-food resources, such as oils with a high content of fatty acids, to serve as biodiesel feedstock. This paper examines the potential use of oil with high fatty acid content, specifically palm fatty acid distillate (PFAD), as a biodiesel feedstock. Typically, the biodiesel production process from PFAD involves a two-step catalytic process that requires a strong acid catalyst, known for its corrosiveness, that needs costly equipment materials. This research explores a catalyst-free production process ensuring a non-corrosive process with reduced capital expenses and fewer chemicals, thereby lowering operational costs. The results demonstrate that biodiesel from PFAD can be obtained in concentration of 83.9% using a one-step non-catalytic process with superheated methanol vapor at a temperature of 210°C under atmospheric pressure. This highlights the significant potential of catalyst-free technology in the production of biodiesel from PFAD.

Evaluation of a novel palm-free fat supplement to reduce the carbon footprint of diets for dairy cows

Most fat supplements added to dairy diets are derived from palm acid oil or palm fatty acid distillate. There are environmental concerns about palm oil production due to deforestation and high greenhouse gas (GHG) emissions. The objective of this study was to evaluate a palm-free fat supplement, Envirolac. Envirolac contains vegetable oils, marine oils and glycerine encapsulated within a cellulosic fibre-clay mineral carrier matrix. The carrier matrix has small particle size to facilitate rapid rumen passage and minimal interference with rumen digestion. Fifty cows were divided into two balanced groups of 25 cows. Each group received Control (calcium soap of palm fatty acid distilate, 0.5 kg/d) and Envirolac (0.5 kg/d) diets in a crossover design with two feeding periods of four weeks duration, so that each cow received both diets. Envirolac has a lower total fat concentration than the calcium soap, so the feeding rate delivered less total fat. When fed on Envirolac, cows yielded more milk 40.7 v 40.1 kg/d), energy-corrected milk (ECM; 42.9 v 41.3 kg/d) and milk components (1605 v 1514 g fat, 1239 v 1199 g protein, 1871 v 1839 g lactose, per day), and produced milk with higher concentrations of fat (40.1 v 38.6 g/kg) and protein (30.7 v 30.1 g/kg), than when fed on Control. When fed on Envirolac, cows produced milk with higher concentrations of fatty acids (FA) synthesised de novo in the mammary gland (25.5 v 23.6 g/100 g total FA), lower concentration of palmitic acid (35.5 v 37.2 g/100 g total FA), and higher concentrations of some long-chain fatty acids (C20:0, C20:1, C20:3n3, C21:0, C22:0, C22:6n3 and C23:0), than when fed on Control. There was no effect of treatment on dry matter intake (DMI; mean 23.4 kg/d), so feed efficiency was higher (1.83 v 1.76 kg ECM/kg DMI) for Envirolac than Control. There was no effect of treatment on dry matter digestibility (0.73), methane production (433 g/d), methane yield (19.3 g/kg DMI) or methane intensity (10.9 g/kg ECM). Feed carbon footprint of Envirolac was calculated to be 1028 g CO2eq/kg DM, which is 0.46 of the value for a calcium soap (2830 g CO2eq/kg DM), and reduced feed carbon footprint per kg ECM milk production by 11 %. This study demonstrates that Envirolac can replace palm-based fat supplements in dairy diets to improve feed efficiency and reduce the carbon footprint of milk production.

БАЛЫҚ ДЕРНӘСІЛДЕРІН ӨСІРУ ҮШІН ОҢТАЙЛЫ ҚОРЕК РЕСУРСЫ РЕТІНДЕ АРТЕМИЯЛАРДЫ ӨНДІРУ

In aquaculture, the use of Artemia as an optimal nutrient resource for fish fry is an object of growing interest. The advantages of using Artemia include the possibility of mass cultivation under controlled conditions, the possibility of long-term storage in frozen form and resistance to external environmental conditions. Artemia, belonging to crustaceans, is a rich source of protein, fat, vitamins and minerals necessary for the healthy development of fish larvae. Due to the high content of unsaturated fatty acids, omega-3 and omega-6, fry contribute to the development of a healthy immune system, which reduces the risk of many diseases. Their small size and mobility make them available for cultivation, which ensures a high level of quality and reduces feed consumption. The results of the study show that the use of crustaceans as the main food source for fish farming contributes to improving the life, growth and health of fry. This makes them a successful type of feed in aquaculture and an optimal resource for fish farming. Further research in this area can lead to increased efficiency of fish farming, development of practices and improvement of the overall potential of the aquaculture industry. This article discusses the advantages of using Artemia, its biological value, ease of cultivation and versatility in the nutrition of various fish larvae. Various methods and technologies of growing Artemisia are analyzed, as well as their application in breeding species of fish larvae. Special attention is paid to optimizing the maximum growth of fish larvae and feeding conditions.

TRIM45 facilitates NASH-progressed HCC by promoting fatty acid synthesis via catalyzing FABP5 ubiquitylation.

Non-alcoholic steatohepatitis (NASH) is rapidly surpassing viral hepatitis as the primary cause of hepatocellular carcinoma (HCC). However, understanding of NASH-progressed HCC remains poor, which might impede HCC diagnosis and therapy. In this study, we aim to identify shared transcriptional changes between NASH and HCC, of which we focused on E3 ligase TRIM45. We found TRIM45 exacerbates HCC cells proliferation and metastasis in vitro and in vivo. Further transcriptome analysis revealed TRIM45 predominantly affects fatty acid metabolism and oleic acid restored impaired proliferation and metastasis of TRIM45-deficient HCC cells. IP-tandem mass spectrum and FABP5 depriving experiment indicated that TRIM45 enhance fatty acid synthesis depending on FABP5 presence. Interestingly, we found TRIM45 directly added K33-type and K63-type poly-ubiquitin chains to FABP5 NLS domain, which ultimately promoted FABP5 nuclear translocation. Nuclear FABP5 interacted with PPARγ to facilitate downstream lipid synthesis gene expression. We observed TRIM45 accelerated NASH-to-HCC transition and exacerbated both NASH and NASH-HCC with the enhanced fatty acid production in vivo. Moreover, high concentration of fatty acid increased TRIM45 expression. The established mechanism was substantiated by gene expression correlation in TCGA-LIHC. Collectively, our research revealed a common lipid reprograming process in NASH and HCC and identified the cyclical amplification of the TRIM45-FABP5-PPARγ-fatty acid axis. This signaling pathway offers potential therapeutic targets for therapeutic intervention in NASH and NASH-progressed HCC.

Gamma rays and sodium azide induced variations in bio-physiological and agronomical traits in linseed (Linum usitatissimum L.)

Linseed is a valuable oilseed crop with huge therapeutic importance due to its high content of omega-3 fatty acids in the form of Alpha-linolenic acid (ALA). It is a self-pollinated crop with a low-yielding potential that restricts its improvement endeavors. To overcome low-yielding potential, individual and combination treatments of gamma rays and sodium azide were employed in widely grown linseed varieties. The results revealed a dose-dependent decline in seed germination, seedling height, pollen fertility, chlorophyll, and carotenoid contents and a dose-independent decline in carbonic anhydrase activity. Bio-physiological parameters decreased substantially in combination treatments compared to individual treatments of gamma rays and sodium azide. In contrast, lower doses of gamma rays, sodium azide, and their combinations effectively increased mean values of yield and yield-attributing traits in a few putative mutants. Such putative mutants represent a valuable genetic resource that could be used in future breeding programs for the genetic improvement of linseed and related medicinal plants.

β-sitosterol alleviates high fatty acid-induced lipid accumulation in calf hepatocytes by regulating cholesterol metabolism

A significant reduction in plasma concentration of cholesterol during early lactation is a common occurrence in high-yielding dairy cows. An insufficient synthesis of cholesterol in the liver has been linked to lipid accumulation caused by high concentrations of fatty acids during negative energy balance (NEB). As ruminant diets do not provide quantitative amounts of cholesterol for absorption, phytosterols such as β-sitosterol may serve to mitigate the shortfall in cholesterol within the liver during NEB. To gain mechanistic insights, primary hepatocytes were isolated from healthy female 1-day old calves for in vitro studies with or without 1.2 mM fatty acids (FA) to induce metabolic stress. Furthermore, hepatocytes were treated with 50 μM β-sitosterol with or without FA. Data were analyzed by one-way ANOVA with subsequent Bonferroni correction. Results revealed that calf hepatocytes treated with FA had greater content of non-esterified fatty acids (NEFA) and triacylglycerol (TAG), and greater mRNA and protein abundance of the lipid synthesis-related SREBF1 and FASN. In contrast, mRNA and protein of CPT1A (fatty acid oxidation) and the cholesterol metabolism-related targets SREBF2, HMGCR, ACAT2, APOA1, ABCA1 and ABCG5 was lower. Content of the antioxidant-related glutathione (GSH) and activities of superoxide dismutase (SOD) also was lower. Compared with FA challenge alone, 50 μM β-sitosterol led to greater mRNA and protein abundance of SREBF2, HMGCR, ACAT2 and ABCG5, and greater content of GSH and activity of SOD. In contrast, compared with the FA group, the mRNA and protein abundance of SREBF1 and ACC1 and the content of TAG and NEFA in the β-sitosterol + FA group were lower. Overall, β-sitosterol can promote cholesterol metabolism and reduce oxidative stress while reducing lipid accumulation in hepatocytes challenged with high concentrations of fatty acids.

A Mediterranean diet improves glycation markers in healthy people and in those with chronic diseases: a systematic review of clinical trials.

Consumption of the Mediterranean diet (MedDiet) has beneficial effects on cardiometabolic health and aging. This systematic review aimed to critically investigate the effect of the MedDiet on glycation markers in healthy or overweight individuals with type 2 diabetes or cardiovascular disease. MEDLINE, EMBASE, Web of Science, and the Cochrane Library were searched, using the terms "Mediterranean diet" AND "glycation end products, advanced". Three randomized and 3 nonrandomized clinical trials, containing data on 2935 adult and elderly individuals with normal weight or overweight, were included. All extracted data were compiled, compared, and critically analyzed. The authors of most of the studies demonstrated a reduction in serum concentrations of advanced glycation end products (AGEs), such as εN-carboxymethyllysine and methylglyoxal, and in skin autofluorescence levels after at least 4 weeks of adherence to the MedDiet. The MedDiet also led to positive effects on gene expression of receptors for AGEs, as RAGE and AGER1, and an enzyme involved in detoxification (glyoxalase I). There is no evidence that short-term adherence affects glycation markers. Glycation markers improved in response to the MedDiet. The possible mechanisms involved may be related to the low AGE and refined sugars content of the diet, as well as its high monounsaturated fatty acid, phenolic compound, and dietary fiber contents. PROSPERO registration no. CRD42021284006.

Combination of addition and activation of lipase enzyme in peanut seed (Arachis hypogaea L.) for direct fatty acid production

Fatty acids are oleochemical products that are widely used as raw materials in various industries. Fatty acids are obtained from various plants and animals, one of which is peanuts. Peanuts contain 76% - 82% unsaturated fatty acids and the rest is saturated fatty acids. The purpose of this study is to produce fatty acids by turning on the peanut seed’s lipase enzyme. In this study, the addition of immobilized lipase enzyme as much as 0.1% of the sample mass was also carried out. This research method was carried out by grinding peanut seeds using a chopper with variations in the addition of water and variations in reaction time at 35 °C and 30 °C. The mixture of peanut seeds and water is then separated using a hand press, then heated to evaporate the water content. Furthermore, the resulting peanut oil was tested for fatty acid content, density, viscosity, and pH. From this study, the highest fatty acid content was obtained at 10.73% which was achieved at 35 °C and at a reaction time of 6 hours with the addition of enzymes and the addition of 40% water, while the result obtained without the addition of enzymes at the same conditions was 4.51%.

Hemp Seeds (Cannabis sativa L.) as a Valuable Source of Natural Ingredients for Functional Foods-A Review.

Hemp (Cannabis sativa L.) has experienced a significant resurgence in popularity, and global interest in diversifying its use in various industries, including the food industry, is growing. Therefore, due to their exceptional nutritional value, hemp seeds have recently gained increasing interest as a valuable ingredient for obtaining high-quality foods and dietary supplements. Hemp seeds stand out for their remarkable content of quality proteins, including edestin and albumin, two distinct types of proteins that contribute to exceptional nutritional value. Hemp seeds are also rich in healthy lipids with a high content of polyunsaturated fatty acids, such as linoleic acid (omega-6), alpha-linolenic acid (omega-3), and some vitamins (vitamins E, D, and A). Polyphenols and terpenoids, in particular, present in hemp seeds, provide antimicrobial, antioxidant, and anti-inflammatory properties. This review examines the scientific literature regarding hemp seeds' physicochemical and nutritional characteristics. The focus is on those characteristics that allow for their use in the food industry, aiming to transform ordinary food products into functional foods, offering additional benefits for the body's health. Innovating opportunities to develop healthy, nutritionally superior food products are explored by integrating hemp seeds into food processes, promoting a balanced and sustainable diet.

Study on Ways to Improve the Quality of Black Goat Meat Jerky and Reduce Goaty Flavor through Various Spices.

In this study, we analyzed the physicochemical and sensory properties of black goat jerky marinated with various spices (non-spice, control; rosemary, RO; basil, BA; ginger, GI; turmeric, TU; and garlic, GA). The physicochemical properties of black goat jerky analyzed were pH, water holding capacity, color, cooking yield, shear force, and fatty acid composition. The sensory characteristics were analyzed through the aroma profile (electronic nose), taste profile (electronic tongue), and sensory evaluation. The pH and water holding capacity of the GI showed higher values than the other samples. GI and GA showed similar values of CIE L* and CIE a* to that of the control. The shear force of the GI and TU was significantly lower than that of other samples (p<0.05). Regarding fatty acid composition, GI showed high unsaturated and low saturated fatty acid contents compared with that of the other samples except for RO (p<0.05). In the aroma profile, the peak area of hexanal, which is responsible for a faintly rancid odor, was lower in all treatment groups than in the control. In the taste profile, the umami of spice samples was higher than that of the control, and among the samples, GI had the highest score. In the sensory evaluation, the GI sample showed significantly higher scores than the control in terms of flavor, aroma, goaty flavor, and overall acceptability (p<0.05). Therefore, marinating black goat jerky with ginger powder enhanced the overall flavor and reduced the goat odor.

Preparation and coating properties of alkyd polyol-based autoxidizable waterborne polyurethane dispersions with high fatty acid content, long storage stability, and low viscosity

Preparation and coating properties of alkyd polyol-based autoxidizable waterborne polyurethane dispersions with high fatty acid content, long storage stability, and low viscosity

Shoots and Turions of Aquatic Plants as a Source of Fatty Acids.

Fatty acids are essential for human health. Currently, there is a search for alternative sources of fatty acids that could supplement such sources as staple crops or fishes. Turions of aquatic plants accumulate a variety of substances such as starch, free sugars, amino acids, reserve proteins and lipids. Our aim is to see if turions can be a valuable source of fatty acids. Overwintering shoots and turions of aquatic carnivorous plants were collected. The plant material was extracted with hexane. The oils were analyzed using a gas chromatograph with mass spectrometer. The dominant compound in all samples was linolenic acid. The oil content was different in turions and shoots. The oil content of the shoots was higher than that of the turions, but the proportion of fatty acids in the oils from the shoots was low in contrast to the oils from the turions. The turions of Utricularia species were shown to be composed of about 50% fatty acids. The turions of Utricularia species can be used to obtain oil with unsaturated fatty acids. In addition, the high fatty acid content of turions may explain their ability to survive at low temperatures.

Comparative analysis on the properties of egg yolk lipids extracted by different extraction methods.

Egg yolk lipids are important components in egg yolk. Compared with proteins, minerals, vitamins and pigments in egg yolk, there is a lack of systematic research on extraction methods and characteristics of yolk lipid. Therefore, in this study, the extraction of egg yolk lipids by supercritical CO2 fluid extraction (SFE), subcritical propane extraction (SPE) and ethanol solvent extraction (SE) was studied, and the differences in composition and physicochemical properties of the three yolk lipids were analyzed. The egg yolk lipids extracted by SFE had the advantages of high unsaturated fatty acid content, low saponification value, acid value, peroxide value, and high content of neutral lipids such as triglyceride and cholesterol, but the polar phospholipids were completely retained in the yolk powder. The efficiency of lipid extraction of SPE was the highest among all the three methods, but the extraction capacity to the polar phospholipid was in the middle of the three methods. The efficiency of lipid extraction of SE was low, but the extraction capacity to the polar phospholipid was the highest. Suitable extraction methods could be selected based on the specific application requirements of egg yolk lipids and low-fat egg yolk powders.

Lipidomic and transcriptomic analysis of triacylglycerol accumulation mechanism of pecan (Carya illinoinensis) nut

Pecan (Carya illinoinensis) holds significant prominence as an oil-seed nut, characterized by its high content of fatty acids and triacylglycerol (TG). The inclusion of TG in the C. illinoinensis nut is of paramount importance, not only contributing significantly to the nutritional value for human consumption but also serving as a reliable and eco-friendly resource for various industrial uses. The present study conducted a comprehensive analysis involving transcriptomics, lipidomics, and physiological assessments to explore the dynamic patterns and components of triacylglycerols (TG) in C. illinoinensis nuts throughout the ripening phase. Additionally, the investigation aimed to elucidate the molecular mechanisms governing TG metabolism. The physiological investigation conducted on the maturation progression of three cultivars of C. illinoinensis unveiled distinctive traits within each variety. Notably, ‘Mahan’ displayed elevated concentrations of oil and crude fat, in contrast to the contrasting trend observed in ‘Y-01’. Further lipidomic scrutiny exposed that ‘Mahan’ exhibited augmented levels of glycerides, specifically triglycerides (TG), monoacylglycerol (MG), and diacylglycerol (DG), compared to its counterparts during the ripening phase. The comparative analysis of RNA-Seq libraries uncovered a notable increase in unigenes associated with the ‘glycerolipid metabolism’ pathway. Moreover, the amalgamation of transcriptomic and lipidomic information indicated a heightened expression of genes implicated in TG metabolism in ‘Mahan’. Moreover, there was a strong association between CiGPAT3, CiDGAT1, CiTAGL2, and CiMAGL1 genes and TG metabolism during the ripening process of C. illinoinensis nuts. Utilizing weighted gene co-expression network analysis (WGCNA) identified six distinct modules linked to various ripening stages, alongside the discovery of ten transcription factor families implicated in regulating TG metabolism. In summary, our research provides significant insights into the molecular framework that governs the accumulation mechanism of triglycerides (TG) in C. illinoinensis nuts throughout the ripening phase, which plays a pivotal role in optimizing the yield of C. illinoinensis oil and fostering progress in the molecular breeding.

A telomere-to-telomere gap-free reference genome assembly of avocado provides useful resources for identifying genes related to fatty acid biosynthesis and disease resistance.

Avocado (Persea americana Mill.) is an economically valuable plant because of the high fatty acid content and unique flavor of its fruits. Its fatty acid content, especially the relatively high unsaturated fatty acid content, provides significant health benefits. We herein present a telomere-to-telomere gapless genome assembly (841.6Mb) of West Indian avocado. The genome contains 40 629 predicted protein-coding genes. Repeat sequences account for 57.9% of the genome. Notably, all telomeres, centromeres, and a nucleolar organizing region are included in this genome. Fragments from these three regions were observed via fluorescence in situ hybridization. We identified 376 potential disease resistance-related nucleotide-binding leucine-rich repeat genes. These genes, which are typically clustered on chromosomes, may be derived from gene duplication events. Five NLR genes (Pa11g0262, Pa02g4855, Pa07g3139, Pa07g0383, and Pa02g3196) were highly expressed in leaves, stems, and fruits, indicating they may be involved in avocado disease responses in multiple tissues. We also identified 128 genes associated with fatty acid biosynthesis and analyzed their expression patterns in leaves, stems, and fruits. Pa02g0113, which encodes one of 11 stearoyl-acyl carrier protein desaturases mediating C18 unsaturated fatty acid synthesis, was more highly expressed in the leaves than in the stems and fruits. These findings provide valuable insights that enhance our understanding of fatty acid biosynthesis in avocado.