Combination of addition and activation of lipase enzyme in peanut seed (Arachis hypogaea L.) for direct fatty acid production

Abstract

Fatty acids are oleochemical products that are widely used as raw materials in various industries. Fatty acids are obtained from various plants and animals, one of which is peanuts. Peanuts contain 76% - 82% unsaturated fatty acids and the rest is saturated fatty acids. The purpose of this study is to produce fatty acids by turning on the peanut seed’s lipase enzyme. In this study, the addition of immobilized lipase enzyme as much as 0.1% of the sample mass was also carried out. This research method was carried out by grinding peanut seeds using a chopper with variations in the addition of water and variations in reaction time at 35 °C and 30 °C. The mixture of peanut seeds and water is then separated using a hand press, then heated to evaporate the water content. Furthermore, the resulting peanut oil was tested for fatty acid content, density, viscosity, and pH. From this study, the highest fatty acid content was obtained at 10.73% which was achieved at 35 °C and at a reaction time of 6 hours with the addition of enzymes and the addition of 40% water, while the result obtained without the addition of enzymes at the same conditions was 4.51%.