Concentrations Of Growth Regulators Research Articles

FEATURES OF IN VITRO CULTIVATION OF ASTRAGALUS DASYANTHUS PALL PLANTS

The research is devoted to the study of the peculiarities of in vitro cultivation of the medicinal plant Astragalus dasyanthus Pall, which is listed in the Red Book of Ukraine and has the status of an endangered species. The cultivation of woolly-flowered astragalus in Ukraine is currently difficult due to the inaccessibility of the raw material base in the temporarily occupied southern part of Ukraine, as well as due to the low seed productivity of the crop. The use of in vitro plant tissue cultivation methods to restore natural communities of A. dasyanthus and expand possible sources of raw materials for the synthesis of medicinal substances is promising. In this work, we investigated the conditions for obtaining aseptic Astragalus dasyanthus plants from seeds and evaluated the effect of preliminary mechanical scarification on its germination energy, taking into account the percentage of sterile seedlings obtained. In addition, the efficiency of microclonal propagation of A. dasyanthus was determined depending on the composition of the culture medium, in particular the concentration of macronutrients and the presence and concentration of growth regulators. It has been shown that aseptic plants of Astragalus dasyanthus can be obtained by surface sterilization of seeds that have been previously subjected to mechanical scarification or by germination on Murashige and Skoog nutrient medium with the addition of 0.1 mg/l benzylaminopurine. It was determined that the type and concentration of cytokinin affects the efficiency of microclonal propagation of A. dasyanthus plants, while halving the content of macronutrients in the nutrient medium does not significantly affect this item. It was found that the addition of benzylaminopurine to the culture medium stimulates the formation of more additional shoots of A. dasyanthus in vitro compared to the culture medium containing kinetin. Thus, Murashige and Skoog medium with the addition of 0.5 mg/l of benzylaminopurine is optimal for in vitro cultivation of woolly-flowered Astragalus plants.

THE INFLUENCE OF GROWTH REGULATORS ON THE SOWING QUALITIES OF EUROPEAN LARCH SEEDS IN THE CONDITIONS OF THE BOTANICAL GARDEN «PODILLYA»

The article highlights the main aspects of the use of growth regulators in the cultivation of European larch seedlings by seed. In the research material of the article, laboratory studies were conducted to determine the effect of different concentrations of growth regulators on the formation of germination and germination energy of European larch seeds. As a result of the research, it was found that the minimum concentration of agrostimulin also increased germination energy by 3.5 % and laboratory germination by 9.6 % compared to the control. It was determined that the use of higher concentrations of agrostimulin concentrations (1 and 2 ml/l) led to an increase in germination energy by 7.8 % and 11.5 %, respectively, and to an increase in germination rate by 14.7 % and 20 %. It was found that the concentration of 2.0 ml/l of emistim-C was the most effective, increasing germination energy by 12.1% compared to the control variant, and laboratory germination reached its maximum value at a concentration of 2 ml/l and exceeded the control by 15.9%. The study revealed that an increase in the concentration of PABA solutions contributes to an increase in germination energy and laboratory germination of European larch seeds. The most pronounced effect is observed at a concentration of 100 mg/l and is 59.6 % and 72.8 %, respectively. The results of the study show that solutions of Triman-1 in concentrations of 25 mg/l and 50 mg/l have a positive effect on germination energy, increasing it by 9% and 9.9% and laboratory germination of European larch seeds by 12.3% and 13.9%, respectively. Also in the course of the research, it was determined that excessive concentrations of different types of growth regulators gave insignificant results compared to the control, which indicates the ineffectiveness of their use.

IN VITRO PRODUCTION OF VIRUS-FREE CARNATION (DIANTHUS CARIOPHYLLUS L.) PLANTING MATERIAL

The methods of culture of apical meristems and direct and indirect morphogenesis in vitro were used for production of virus-free planting material of carnation. A scheme for obtaining aseptic material has been developed, which consists of stepwise treatment of explants: Thimerosal - 2 min, 70% ethyl alcohol - 0.5 min and 0.08% AgNO 3 - 1 min, which reduces the level of contamination by fungal infection. Expounded the results of studies of callusogenesis and direct and indirect morphogenesis in the culture of in vitro explants of Dutch carnation, their dependence on the content of growth regulators in the nutrient medium. It was established that there were almost no significant differences in the course of callusogenesis processes within carnation varieties . At the same time, the frequency of callusogenesis was 100%. Under the conditions of indirect morphogenesis realization, it is necessary to take into account the age of callus tissues. The growth and intensive shoot formation of carnations was noted on the Murashige-Skoog nutrient medium supplemented with BAP at a concentration of 0.5 mg/l. The best medium for rooting was the MS medium with half the concentration of macro- and microsalts with the addition of 0.5 mg/l of NAA, which is recommended by us for rooting regenerating carnation plants of various varieties. Peat : perlite in a 1:1 ratio was used as a substrate for the adaptation of regenerating plants . Survival of carnation plants to conditions in vivo for the variety "Raffino Linde" was 90%, while for the variety "Tiya" - 83%, respectively.

Propagação rápida da planta ornamental Homalomena gigantea usando estacas de rizoma

Abstract This study investigates the critical factors influencing shoot and root regeneration from Homalomena gigantea rhizome cuttings, providing valuable insights for rhizome cutting practices for this plant species. Three key experiments were conducted to assess the impact of plant growth regulator (PGR) concentrations, cutting length, and cutting section on regeneration. In the first experiment, different concentrations of α-NAA and BAP were examined, revealing that PGR selection and concentration play a pivotal role in shoot and root induction and plantlet development. The highest success rates were observed at 150 ppm α-NAA and 100 ppm BAP, although there was a trade-off between the quantity and quality of plantlets, emphasizing the need for a balanced plant regulator selection. The second experiment investigated the influence of rhizome cutting length. Longer cuttings, particularly at 5 and 7 cm, consistently led to better shoot and root development, highlighting the importance of selecting appropriate cutting lengths. In the third experiment, cutting sections (apical, middle, and distal) without PGR concentrations were assessed, with all sections showing high regeneration rates. However, the apical section produced the highest-quality plantlets with superior growth parameters, followed by the middle section, while the distal section exhibited lower effectiveness. In summary, these findings underscore the significance of PGR choice and concentration, cutting length, and cutting section for successful regeneration of H. gigantea from rhizome cuttings. These insights provide valuable guidance for enhancing rhizome cuttings methods for this plant species and promoting efficient propagation.

The appearance of amyloplasts as one of the features distinguishing the initial stages of the development of isolated endosperm in kiwiberry

The potency of plant cells’ reaction to dedifferentiation and then proliferation corresponds with cell reorganization. The details of cell modifications may rely on the fate of the cells. In this study, the characteristics of an endosperm and specific storage tissue under organogenic and non-organogenic development pathways were analyzed. The experimental system was based on isolated endosperm explants (with the seed coat and embryo removed) of kiwiberry cv. Bingo, placed on organogenic (OCIM) and non-organogenic (NOCIM) callus induction media that differed in the type and concentration of plant growth regulators. Histological and ultrastructural analyses of freshly isolated endosperm and explants afer selected periods of the culture were performed using stereoscopic, bright field, and transmission electron microscopy techniques. The dedifferentiation for both pathways– organogenic and non-organogenic – was similar at the morphological level for the first week of the culture. The histological and ultrastructural analyses revealed limited amyloplasts in the cells on OCIM. e organization of proliferated tissue differed significantly from two weeks of the culture: it was more compact for OCIM and looser for NOCIM. Additionally, at this period, there were more amyloplasts with starch granules in dedifferentiated cells on OCIM. The limited starch granules in proliferated cells on NOCIM were observed only afer four weeks of the culture. The presence of plastids with starch granules depends on the competence of endosperm-derived callus in kiwiberry to organogenic processes and can be an early marker of callus destination.

In Vitro Seed Germination and Seedling Development of Mimusops laurifolia (Forssk.) Friis.: An Endangered Plant Species

Mimusops laurifolia (Forssk.) Friis is an endangered medicinal plant and natural propagation of this plant through seeds is found to be very difficult due to its hard seed coat. To conquer this problem, an efficient in vitro seed germination as well as subsequent in vitro regeneration technique has been developed in conserving this endangered plant species. Seed germination was achieved on half strength of MS (HMS), full strength MS (FMS) and MS medium supplemented with different concentration and combination of Plant Growth Regulators (PGR) like BA, Kn and GA. The highest seed germination rate (18%) was obtained from water soaking de-coated seeds inoculated on full strength of MS (FMS), on the other hand, 12% germination of seeds was recorded on HMS medium, but both the conditions required more than 90 days to germinate. A similar rate of germination was also found on MS with PGRs, but it required 55-60 days. Best responses towards seed germination with elongated shoots (2.8 cm and 3.0 cm) were obtained when seeds were inoculated on MS with 3.0 mg/l BA, 0.5 mg/l Kn and 0.2 mg/l GA. After a long period of period of 8 months following germination, seedlings produced elongated tap roots only. Rooted seedlings were successfully transferred to soil for their further growth and development. Apart from this, for in vitro regeneration of shoots, explants of nodal segments and shoot tips from the natural plant were cultured on woody plant medium (WPM) supplemented with various combination and concentration of BAP, Kn and TDZ. In these cases, there was no positive response recorded towards the initiation of shoots from nodal segment and shoot tips. Plant Tissue Cult. & Biotech. 33(2): 143-153, 2023 (December)

Micropropagation from cotyledonary explants of grapefruit (<i>Citrus</i> <i>paradisi</i> Macf.) by indirect and direct organogenesis

Grapefruit (Citrus paradisi Macf.) is an economic fruit crop worldwide cultivated for its nutritional value, and aromatic flavours. However, the development of new varieties and high production of quality seedling constitutes a limit to improving grapefruit yields. An efficient regeneration protocol could be of great interest for grapefruit transformation, micropropagation, and germplasm conservation. In vitro plant regeneration systems of Citrus paradisi have been studied by using cotyledonary explants. Different concentrations of plant growth regulators were used to explore the organogenic potential of cotyledonary explants under laboratory conditions. For indirect organogenesis using cotyledon segment explants, a low concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) lead to the formation of white callus, while a high concentration of 2,4-D induced brown callus and adventitious root differentiation. The best response for callogenesis was obtained on Murashige and Skoog (MS) medium supplemented with 2 mg/L of 2,4-D. Only brown callus cultured on MS added with 2 mg/L 6-benzylaminopurine (BAP) has regenerated shoots, but the percentage of callus producing shoots was very low (6.66 ± 2.68%). Regarding direct organogenesis from the cotyledonary node explants, the best response for shoot proliferation was observed on MS supplemented with 4 mg/L BAP (86.66 ± 12.58% of explants regenerating shoots, and an average number of 5.45 ± 0.45 shoots per explant). Low percentages of In vitro rooting of regenerated shoots were observed with different concentrations of α-naphthalene acetic acid (NAA) or indole-3-butyric acid (IBA). Nevertheless, the highest rooting percentage (13.33 ± 2.88%) was obtained on MS containing 2 mg/L NAA. These results provide new information for mass regeneration and the production of healthy seedlings, and can be applied in grapefruit improvement programs.

Effect of anti-ethylene and cytokinin biostimulants on flowering and yield of Sweetpotato (Ipomoea batatas L.)

Breeding is needed to enhance the poor agronomic and qualitative attributes of the majority of sweetpotato cultivars grown in Nigeria. Even though the majority of cultivars don't often flower, the flowers are essential for genetic advancements. This study aimed to assess the effect of anti-ethylene (Silver-thiosulphate) and cytokinin (6-benzyladenine) biostimulants for induction of flowering and yield, to encourage early flowering of delayed sweetpotato genotype, and to ascertain the ideal concentration of growth regulators for sweetpotato flowering induction. A randomized complete block design with three replications was fitted with a 3x3x2 factorial arrangement. The first factor consisted of three different concentrations of two plant growth regulators (PGR): silver thiosulphate (STS) and 6-benzyladenine (BA) (0, 50, and 100 ppm). Three types of sweet potatoes (Umuspo3, Buttermilk, and Tis87) constituted the second factor. After planting, the BA and STS were administered three weeks later. The sweet potato varieties sprayed with STS and BA displayed morphological and physiological challenges, such as stem drooping (Temporal) (Page 1A), which recovered in 24 hours, root swelling, stem splitting, bud and flower production, vine branching, and elongation (Plate 1B). Varieties sprayed with high concentrations of STS (100 ppm) displayed significant morphological and physiological abnormalities, while those sprayed with BA displayed a significant increase in all growth metrics. However, two of the varieties Umuspo3 and Tis87 that were sprayed with STS and BA began buds and set flowers within two weeks after the treatment, whereas the plants that were not sprayed did not flower at all. The results of the interactions showed that the combination of benzyladenine + Umuspo3 at 100% (100T1V1) was superior for yield, whereas the combination of silver thiosulphate + Umuspo3 at 100% (100T2V1) was superior for the total number of flowers/plant. The vegetative growth parameters and the flowering characteristic exhibited a substantial positive connection (P<0.05), however the correlation between the vegetative growth traits and the yield traits was negative. As a result, the higher dosage of BA and STS (100 ppm) that was employed in this experiment is most likely quite near to the ideal concentration for sweet potato flower induction. Despite not being the ideal concentration, sweet potatoes can currently be induced to flower at this level, enabling the start of breeding programs for the crop.

Standardization of Meta-Topolin Concentration for Maximizing In vitro Proliferation in Commercial Banana Cultivars of Andhra Pradesh, India

Cytokinins are associated with cell division, cell growth, and differentiation, are the most common plant growth regulators used in micropropagation. Aromatic cytokinin, meta-topolin (mT), and its derivatives have been demonstrated as alternative cytokinins in the in vitro multiplication of various plants. Different banana cultivars differ in in vitro shoot proliferation due to the difference in endogenous growth regulator content among them. A study was conducted to figure out the effect of different concentrations of meta-topolin on in vitro shoot proliferation of banana cultivars like Karpura chakkera keli (AAB), Mortoman (AAB), and Kovvur Bontha (ABB). In vitro shoot proliferation was initiated from the first subculture (C1) itself at 1.5 ppm mT concentration in Mortoman, Kovvur Bontha, and from the second subculture (C2) at 1.0 ppm mT concentration in Kovvur Bontha. Whereas, at all other remaining concentrations, shoot proliferation of different cultivars was initiated from the third subculture (C3) only. At the end of the sixth subculture (C6), the maximum number of shoots per explant (82.70) was recorded in the Kovvur Bontha cultivar at 1.5 ppm mT concentration. Among the cultivars, the Kovvur Bontha cultivar responded better to the meta-topolin for shoot proliferation than Karpura chakkera keli (AAB) and Mortoman (AAB).

Effect of Indole-3-Butyric Acid (IBA) on Hardwood Cutting of Grapes (Vitis vinifera L.) cv. Pusa Navrang

Aims: The study aims to investigate the influence of different IBA concentration on the rooting and subsequent growth of hardwood cuttings of "Pusa Navrang" grapevine.
 Study Design: The study was performed in RBD design with replicate 5 times.
 Place and Duration of Study: The study was conducted at Research Farm, College of Horticulture, Mandsaur, during the period November 2020- March 21.
 Methodology: Polybags size of 5 X 7 inch was used for experiment. After the filling of growing media in poly bags, the hard wood cuttings of Pusa Navrang with uniform size having 4-5 functional bud was taken from one year matured canes from research farm, College of Horticulture, Mandsaur. The diameter of cutting about 0.75 to 1 cm (pencil thickness) were taken and before planting cuttings were treated with IBA solution by quick dip method.
 Results: The significant result were found for shoot parameter like shoot length, number of nodes per shoot, internodal length, number of buds per shoot, stem diameter, stem fresh weight, stem dry weight. Root Parameters like number of primary and secondary roots, root length, root thickness, whole root volume, fresh weight of root and dry weight of root. Leaf Parameters like number of leaves per plant, fresh weight of leaf, dry weight of leaf, leaf area, leaf area index, specific leaf weight, minimum days taken to emergence of 1st leaf and water content of leaf found in IBA @ 6000 ppm (G3).
 Conclusion: Among four concentration of growth regulator (IBA), the application of IBA @ 6000 ppm (G3) responded well in terms of rooting and shooting in hard wood cutting of grapes.

The effect of exogenous plant growth regulators on elevated Cd phytoremediation by Solanum nigrum L. in contaminated soil.

Maximizing amendment potential is an emphasis in the HM-contaminated field of phytoremediation by hyperaccumulators due to the low bioavailability of HMs in soils and small biomass yields of plants. This study investigated the influence of different types and concentrations of plant growth regulators on Cd phytoremediation by Solanum nigrum in contaminated soil. Our conclusions showed that the shoot Cd extractions (μg plant-1) and the root and shoot biomasses at all the treatments remarkedly increased compared with that of the CK (p < 0.05), while the Cd concentrations at root and aboveground parts by S. nigrum, the extractable Cd concentrations, and pH value of soils did not change significantly compared with the CK (p < 0.05). Furthermore, correlation analysis showed that the shoot Cd phytoaccumulation and the root and aboveground biomasses of S. nigrum were particularly dependent upon the application of CTK and GA3 concentration gradient (p < 0.05). Moreover, some related physicochemical indexes were determined for supervising the growth conditions of plants, and these results pointed out that after exogenous PGRs treatments, the chlorophyll content and antioxidative enzymes POD and SOD activities in vivo of plants clearly advanced, while the H2O2 and MDA contents and CAT apparently declined. These consequence demonstrated that the exogenous PGR addition prominently reinforced the Cd phytoextraction capacity of S. nigrum in contaminated soil by stimulating plant growth and increasing shoot yields.

Growth and Yield of Tomato (Lycopersicum esculentum Mill.) Due to Application of Hormonic PGR and NPK Fertilizer

Tomatoes have high economic value with community demands increases every year. However, the productivity of tomatoes farmers in Indonesia can not meet the demands of Indonesian consumers. Efforts to solve low productivity in tomatoes cultivation can be made through certains cultivation techniques. The intention of this study is to acquire appropriate combination of “Hormonik” Plant Growth Regulator (PGR) concentrations and NPK fertilizer dosages for the optimum growth and yield of tomato plants. This study used a factorial experimental method with a completely randomized design (CRD). The first factor is Hormonik PGR concentrations (H) in 4 different treatment levels that consist of control variable (no PGR added) (H0), 6 ml/l (H1), 7 ml/l (H2), and 8 ml/l (H3). The second factor is NPK fertilizer dosages (N) in 4 different treatment levels that consist of 24 g/plant (N1), 32 g/plant (N2), 40 g/plant (N3), and 48 g/plant (N4). This study showed that combination of 6 ml/l Hormonik PGR and 40 g/plant of NPK fertilizer provide the highest number of fruits per plant by 49.0 and the weight of fruit per plant by 1,572.3 g. Keywords : Concentration of Hormonik PGR, Dose of NPK Fertilizer, Production, Tomato.

The effect of the gibberellic acid on the morphogenesis of Hydrangea L.

Modern ornamental gardening is difficult to imagine without beautifully flowering shrubs. One of these is hydrangea. In Russia, culture is one of the most popular in decorative gardening. However, there are some difficulties in obtaining a large amount of planting material of new and highly ornamental varieties. Therefore, it is important to develop effective reproduction technologies of the studied culture. It is possible to obtain high-quality planting material by clonal micropropagation in a short period of time. The resulting plants grow similar to the mother plant. Hydrangea L. representatives are effectively cultivated in vitro. There are many results of research about micropropagation of hydrangea, but there is only isolated datas about the comparative assessment of the morphogenetic potential of various species of the Hydrangea L. depending on the concentration of growth regulators. There are many growth regulators in the practice of cultivation in vitro. 6-benzylaminopurine (6-BAP), gibberellic acid, kinetin, zeatin. Murashige (MS) and Skoog and Quoirin (QL), Lepoivre nutrient media are used to activate the growth of axillary buds at the stage of micro-reproduction proper. The effect of the use of gibberellins on the in vitro cultivation of hydrangeas is poorly studied. The research aimed to studing the effect of gibberellic acid on the regeneration of shoots of Hydrangea L. We study the effect of gibberellic acid (GA) – 0.5 and 1 mg/l of concentrations. Reproduction coefficient of the Hydrangea macrophylla Thunb. (H. macrophylla) was 12.0, when we used 1 mg / l GA. The length of the Hydrangea paniculata Siebold (H. paniculata) micro-shoots increased by 25 % when we used 1 mg/l GA. According to the research of 2020, the best results on the use of different nutrient mediums mineral bases were obtained during in vitro cultivation on a QL nutrient medium with the addition of 0.3 mg/l 6-BAP. In the future this nutrient medium was as a control. After the application of GA, regenerants were planted to adapt of phytotron conditions. The best survival rate of H. macrophylla (90 %) (var. Peppermint) was recorded after cultivation of a nutrient medium containing 1 mg/l GA. The adaptability of ‘Magical Candle’ was excellent regardless of GA concentration.

Study on gibberellin and gibberellin-like substances from endophytes and their effect on maize plants

Plant growth promoting endophytes were isolated from the roots of rice, sugarcane, corn, maize, sunflower and chili. The biochemical characterization of those isolated endophytes was carried out according to Bergey's Manual of Determinative Bacteriology. The best gibberellin production of all isolates was found at the concentration of (55.12 ppm, 50 ppm, 38.06 ppm, 90.41 ppm, 58.65 ppm and 63.65 ppm, respectively) on the 12th day of the incubation period. Among all isolates, the A4 isolate produced more GA concentration (90.41 ppm) on the 12th day of the incubation period. In the maize seed germination test, A1, A4, A5 and A6 showed the best results. In the pot trial test, A4, A5 and A6 showed the best results in shoot length (37.5 cm, 35.4 cm and 36.9 cm per ten replicates, respectively). Among them, the A4 isolate was the best GA producing isolate for plant growth promoting activity in maize seedlings. It was found in an average shoot height of 37.5 cm; average shoot fresh weight of 56.7 g and an average shoot dry weight of 7.9 g per ten replicates after one month cultivation which was sprayed by A4 treatment, compared with an average shoot height of 27.80 g, an average shoot fresh weight of 25.08 g, an average shoot dry weight of 3.42 g which were sprayed by water treatment. The qualitative analysis of plant growth regulators (GAs) production of bacterial isolate A4 was determined by using TLC method and FTIR spectroscopy analysis. Chemical fertilizers should not be used not to harm effective endophytes.

Influence of Growth Regulators and Tryptophan on Enhancing Production Bioactive Compounds in Callus Cultures of Pistacia weinmannifolia

The experiment was conducted in Tissue culture laboratory at the Biology Department, College of Science ‘University of Al-Qadisiyah. With two explants (the growing apex and the lateral buds), the researchers looked at how the growth regulators (IAA and BA) and Tryptophan affected callus weight and stimulated the synthesis of active substance in Pistacia weinmannifolia plants grown in a tissue culture setting. After sterilization in 70% ethanol solution for 1 minute and 10% sodium hypochlorite (NaOCl) solution for 20 minutes, one node 0.5–1.0 cm in length is planted in test tubes containing MS medium for tissue culture, to which various concentrations of growth regulators and Tryptophan are added, and the tubes are placed in a growth chamber with a temperature of 252 °C and light conditions. Data taken after 45 days of cultivation for all experiments. Better treatment IAA (0.2 mg/L) BA (0.5m.1) and Tryptophan ( 0.1mg/L) gave the highest weight. And significant increase of pentadecanc, Methylox and Catolin. As for treatment IAA ( 0.2mg/L) BA (0.25mg/L) Tryptophan ( 0.1 mg/L) gave highes percentage of active compound Hexadecene.

The of Effects of Light and a Combination of Growth Regulators on the Induction of Somatic Embryogenesis in Orchid Rhynchostylis gigantea (LindI.) Ridl.

The squirrel-tail orchid (Rhynchostylis gigantea) belongs to the Orchidaceae family. This orchid is indigenous to Southeast Asia and is scented and arranged in a bouquet of dangling stems. Its uniqueness makes it commercially desirable. In vitro culture has been utilized for orchid proliferation for a very long time to aid in the development of orchid seedlings. However, not all orchid species react the same way. Through somatic embryogenesis without gamete fusion, in vitro culture techniques can produce new plants during the entire embryonic phase. Endogenous and exogenous hormones, as well as light, influence the success of embryogenesis. Determining the optimal environmental parameters (light, combination and concentration of growth regulators, and their interaction) for inducing somatic embryogenesis from leaf explants in R. gigantea is the purpose of the present study. The leaves of R. gigantea clone 19 were utilized as explants in this investigation, utilizing a completely random factorial design. The first factor is a combination of growth regulator types and concentrations, and the second factor is light. Explants that are alive and growing at a rapid rate indicate that the in vitro culture is successful. Light and the combination of growth regulators significantly affected the percentage of viable explants, the beginning date of callus formation, and the number of embryogenic calluses produced. The interaction of two components (light and a combination of growth regulators) did not affect the three characteristics, with the exception of the proportion of somatic embryogenesis. Incubation in the dark is the optimal environment for initiating somatic embryogenesis in explants. The optimal combination of plant growth regulators for inducing somatic embryogenesis was 0.5 mgL-1 TDZ and 0.1 mgL-1 BAP. Bright light and a concentration of 1.0 mgL-1 TDZ + 0.1 mgL-1 BAP were the optimal interaction conditions for the induction of somatic embryogenesis in R. gigantea.

In Vitro Propagation of Ruta Chalepensis Through and Callus Culture

Plant tissue culture is a field that that enables culturing of various plants and parts of plants usually treated under a nutrient medium and in highly sterile conditions. Out of them callus culture is one of the very interesting arenas of plant biotechnology that encompasses many pivotal benefits. The study focuses on such callus enrichment using different hormones that there by enhance its biological activities. The plant namely Ruta chalepensiswas chosen upon wherein the callus growth was noticed. Ruta chalepensis has multiple medicinal activities like anti-cancer, anti-ulcer, anti-diabetic and many more pharmacological properties that yields in treating and curing of illness. Ruta chalepensis the leaf and internode were taken for the study and to analyse which of those at what concentration of plant growth regulators showed a better callus induction. The MS medium as well as Various hormone concentrations was used for the study like auxin and cytokinin from 0.5mg to 2 mg (2,4 – D, NAA, IAA, IBA). Increased concentration of 2,4-D (1.0 mg/L) alone in the MS medium showed profused callus growth. Both the explants used such as leaf and Internode were also tested in the MS medium which was devoid of hormones/plant growth regulators which was treated as control for comparison. From the data obtained, MS medium supplemented with hormones showed better growth rate and callus induction when compared with that of MS medium without hormones/plant growth regulators. Among the plant growth regulators 2,4-D (1.0 mg/L) showed maximum callus initiation from both leaf and internode explants. Further work was carried out in single and combination of the plant growth regulators for callus proliferation and accumulation. Further analysis is being done to study the growth pattern on combination of hormones and fix the hormone concentrations for the mass propagation of callus from the explants.

Cell culture of Euphorbia fischeriana and enhancement of terpenoid accumulation through MeJA elicitation

Euphorbia fischeriana Steud is a perennial medicinal plant belonging to the Euphorbiaceae family, but its clinical application has been limited due to the scarcity of natural raw materials. Therefore, this study attempted to provide a new method for producing plant materials of E. fischeriana by cell culture. During cell culture, the effects of concentrations of plant growth regulators and sucrose were investigated to improve the culture efficiency. The results showed that the combination of 1.5 mg/L 6-benzylaminopurine and 0.2 mg/L naphthaleneacetic acid, together with a sucrose concentration of 20 g/L in the medium was appropriate for cell growth and terpenoid accumulation. Furthermore, this study applied an elicitation strategy with methyl jasmonate (MeJA) to enhance terpenoid production and investigated the involvement of signaling molecules, such as nitric oxide (NO) and hydrogen peroxide (H2O2). The results indicated that terpenoid accumulation depended on the concentration and treatment duration of MeJA. The maximum total terpenoid content was obtained when the cells were treated with 100 μM MeJA for 4 days. The levels of NO and H2O2 in the cells increased after MeJA treatment and peaked at 8 h and 18 h, respectively, suggesting the involvement of NO and H2O2 in MeJA-induced terpenoid synthesis. The findings of this study demonstrated the potential of cell culture as a method for obtaining plant materials of E. fischeriana.

Pengaruh Ekstrak Bawang Merah (Allium cepa L.) sebagai Zat Pengatur Tumbuh pada Benih Kakao (Theobroma cacao L.)

Cocoa (Theobroma cacao L.) is a beverage crop with high economic value. Provision of natural PGR, namely red unions, which will be extracted. This study aimed to determine the effect of red onionxtract (Allium cepa L.) as a growth regulator on cocoa seeds. The research was conducted at a screen house at the Polytechnic of Agricultural Development Manokwari (Politeknik Pembangunan Pertanian Manokwari). The study method used was a completely randomized design (CRD), using five treatments and 20 replications, so the number of samples was 100 seeds. The treatments tested on cocoa plant seeds P0 (control), P1 (25% concentration of red onion extract), P2 (50% concentration of red onion extract), P3 (75% concentration of red onion extract), and P4 (100% concentration of red onion extract). The measurement variables included germination percentage, plant height, stem diameter, number of leaves, and seedling growth percentage. Differences in the concentration of growth regulators of red onion extract had a significantly different effect on seedling percentage and seed growth percentage and had a significant effect on plant height, stem diameter, and number of leaves of cocoa seedlings. Soaked cocoa seeds in red union extract with a concentration of 25% give optimum results.

Callus Induction of Dendrobium discolor Through The Thin Cell Layer (TCL) Technique Added with 2,4-Dichlorophenoxyaceticacid

Thin Cell Layer (TCL) is a plant propagation technique using thin explants (1-2 mm) through tissue culture. Callus induction is a crucial step in establishing plant regeneration. The type and concentration of growth regulators influence callus formation. 2.4 D is a growth regulator that is commonly used for callus induction in various plants. The research aims to determine the effectiveness of thin explants and 2,4-D in inducing Dendrobium discolor callus. The method used a Completely Randomized Design (CRD) with four treatments (1, 2, 3, and 4 mg/L). Adding 1, 2, and 3 mg/L 2,4-D can induce 100% calluses through the TCL explant. The callus has a friable to compact texture. The result showed that 2 mg/L 2,4-D was the best concentration for callus induction of D. discolor through TCL technique, indicating the greenish color, friable texture, and meristematic tissue.