IN VITRO PRODUCTION OF VIRUS-FREE CARNATION (DIANTHUS CARIOPHYLLUS L.) PLANTING MATERIAL

Abstract

The methods of culture of apical meristems and direct and indirect morphogenesis in vitro were used for production of virus-free planting material of carnation. A scheme for obtaining aseptic material has been developed, which consists of stepwise treatment of explants: Thimerosal - 2 min, 70% ethyl alcohol - 0.5 min and 0.08% AgNO 3 - 1 min, which reduces the level of contamination by fungal infection. Expounded the results of studies of callusogenesis and direct and indirect morphogenesis in the culture of in vitro explants of Dutch carnation, their dependence on the content of growth regulators in the nutrient medium. It was established that there were almost no significant differences in the course of callusogenesis processes within carnation varieties . At the same time, the frequency of callusogenesis was 100%. Under the conditions of indirect morphogenesis realization, it is necessary to take into account the age of callus tissues. The growth and intensive shoot formation of carnations was noted on the Murashige-Skoog nutrient medium supplemented with BAP at a concentration of 0.5 mg/l. The best medium for rooting was the MS medium with half the concentration of macro- and microsalts with the addition of 0.5 mg/l of NAA, which is recommended by us for rooting regenerating carnation plants of various varieties. Peat : perlite in a 1:1 ratio was used as a substrate for the adaptation of regenerating plants . Survival of carnation plants to conditions in vivo for the variety "Raffino Linde" was 90%, while for the variety "Tiya" - 83%, respectively.