Ethanol Concentration Research Articles

Tolerance and efficient metabolization of extremely high ethanol concentrations by a social wasp

Ethanol, a natural by-product of sugar fermentation, can be found in various fruits and nectar. Although many animals routinely consume ethanol in low concentrations as part of their natural diets, its inherent toxicity can cause severe damage. Even species particularly well adapted to ethanol consumption face detrimental effects when exposed to concentrations above 4%. Here, we investigated the metabolism of ethanol and its impact on survival and behavior in the Oriental hornet (Vespa orientalis), a social wasp that naturally consumes ethanol. We show that chronic ethanol consumption, even at concentrations as high as 80%, had no impact on hornet mortality, construction behavior, or agonistic behavior. Using 13C1 labeled ethanol, we show that hornets efficiently metabolized ingested ethanol and at a much higher rate than honey bees. The presence of multiple copies of the alcohol dehydrogenase (NADP+) gene in the Vespa genera suggests a potential mechanism for ethanol tolerance. These findings support the hypothesis that the mutualistic relationship between ethanol-producing organisms and vespid hosts may be at the origin of their remarkable capacity to utilize and metabolize ethanol.

Construction of heterojunction based on Nd2S3 and tin dioxide for rapid detection of ethanol

The gas-sensing properties of Nd2S3 have not been previously explored. In this study, a novel composite material comprising rare-earth metal sulfide (Nd2S3) and metal oxide (SnO2) was successfully prepared using a two-step hydrothermal method for the rapid detection of ethanol. Characterization results revealed that the morphology and specific surface area of Nd2S3-SnO2 remained largely unchanged after composite formation. However, the built-in electric field established between Nd2S3 and SnO2 significantly enhanced carrier transport and separation. Additionally, the introduction of extra oxygen vacancies increased the content of chemisorbed oxygen, providing more active sites for ethanol molecules, thereby improving the gas-sensing performance. The Nd2S3-SnO2 sensor demonstrated extremely high detection accuracy (R2=0.9965) over the 20–300 ppm ethanol concentration range, with response/recovery time of 8/274 s at 100 ppm ethanol concentration and response value of 93. It also possesses excellent selectivity to ethanol, long-term stability and reversibility. This work represents a preliminary exploration of the gas-sensing properties of Nd2S3 and its underlying mechanisms through the construction of a heterojunction structure between metal sulfide and metal oxide.

Isolation and identification of epiphytic Pichia kudriavzevii from loquat peels and investigation of its fermentation characteristics for liquor production.

During the process of fruit wine production, yeast plays a crucial role in influencing the taste, flavor, and overall quality of the wine. This study aims to enhance the flavor and quality of loquat wine by isolating strains of Pichia kudriavzevii (P. kudriavzevii) with desirable winemaking characteristics from loquat fruit fermentation broth. A total of 12 strains of P. kudriavzevii were isolated and subjected to morphological and molecular biological identification. Their fermentation performance, ethanol production, ester production, hydrogen sulfide production, killer activity, and tolerance were evaluated. The results revealed that strains Q-2, Q-9, Q-10, Q-12, Q-20, and Q-42 exhibited robust growth and strong tolerance under conditions of 40°C temperature, 12% ethanol concentration, 350g/L glucose concentration, and pH 2.8. Strain Q-42 demonstrated the strongest gas production capacity, killer activity, and good ester and ethanol production. As a highly active fermentation strain with excellent wine making characteristics, P. kudriavzevii Q-42 provides a valuable yeast resource for the industrial production of loquat wine and offers technical support for improving the overall quality of loquat wine.

Ultrasonic-Assisted Extraction and Antioxidant Evaluation of Resveratrol from Peanut Sprouts

The orthogonal array design method was used to optimize ultrasonic-assisted extraction of resveratrol from peanut sprouts. The results showed that the highest extraction yield of resveratrol using ultrasonic-assisted extraction could be up to 1.1%. The optimal extraction conditions were liquid to solid ratio of 30:1 (mL/g) and ethanol concentration of 80% (v/v) as solvent for 40 min at the temperature of 70 °C. AB-8 macroporous adsorption resin was used to purify the crude extract and the resveratrol content increased to 47.5% after one treatment run. The optimal adsorption parameters were initial concentrations in the sample solution of 2 mg/mL, a pH of 5.0, a flow rate of 2 mL/min, and a temperature of 25 °C. The optimal desorption parameters were 60% ethanol and a flow rate of 1 mL/min. The chemical composition of the peanut sprout’s resveratrol sample was investigated via HPLC, and the predominant constituents were found to be protocatechuic acid, catechins, caffeic acid, epicatechuic acid, resveratrol, and rutin. The antioxidant activities of the resveratrol were measured via the following different analytical methods: reducing power, 2,2-diphenyl-1-picrylhdrazyl (DPPH), hydroxyl radical-scavenger activity, superoxide radical-scavenger activity, the β-carotene bleaching test, and the scavenging nitrite test. The results indicated that the resveratrol in peanut sprouts have significant antioxidant activities and can be used as a source of potential antioxidant. And peanut sprout’s resveratrol has the potential and valuable application to be used as a new type of resveratrol resource. The finding of this study can provide some theoretical reference for the comprehensive utilization of peanut resources in the development of antioxidant health foods.

Composition of Higher Alcohols in Different Alcoholic Beverages and Their Metabolic Dynamics in Bama Pigs.

The unique flavour contribution of higher alcohols in alcoholic beverages has received growing attention; however, there is a dearth of information on their in vivo metabolic kinetics. In this study, the composition and content of higher alcohols in different alcoholic beverages from Chinese Baijiu and Lujiu were studied via in vivo analysis using Bama pigs to elucidate the mechanisms for intoxication of alcohol in vitro and in drinkers. Direct injection combined with gas chromatography-mass spectrometry (GC-MS) were used to accurately quantify a total of 14 higher alcohols in five alcoholic beverages. Based on the external standard method, a total content of 289.37-938.33 mg/L was detected, mainly 1-butanol, 3-methyl-1-butanol, 1-hexanol, 2-methyl-1-propanol and 2-butanol. Then, headspace solid-phase microextraction (HS-SPME) and solid-phase extraction (SPE) combined with GC-MS analysis strategy, respectively, were adopted to continuously monitor the changes in the concentrations of ethanol and 11 higher alcohols in the blood within 24 h after gavage of different alcoholic beverages, and the key pharmacokinetic parameters were analysed. The peak concentration (Cmax) and area under curve (AUC) of blood higher alcohols were significantly lower than those of ethanol (p < 0.05), accompanied by a later peak time (Tmax) and a larger apparent clearance rate (CL_F), and there were certain differences between the same higher alcohols in different alcoholic beverages and between different higher alcohols in the same alcoholic beverage. This work provides valuable insights into the metabolism of alcoholic beverages.

The Role of Keeving in Modulating Fermentation and the Flavour Profiles of Apple Brandy

Keeving is the removal of nutrients from apple musts due to their binding to pectin, resulting in a slower fermentation and spontaneous arrest. The aim of this study was to determine the effect of keeving on the chemical composition of fermented apple must and on the volatile profile and sensory analysis of apple brandies. We compared the application of keeving during spontaneous fermentation with fermentation carried out by Saccharomyces cerevisiae (SafSpirit HG-1). We evaluated the impact of adding different doses of calcium chloride on various parameters of fermented musts and distillates. Calcium chloride had a greater effect on the ethanol concentration, total extract, and fermentation efficiency than on the type of fermentation used. However, a different phenomenon was observed with respect to the volatiles. The concentration of most of the higher alcohols, acetaldehyde, dodecanal, and geranylaceton, decreased after spontaneous fermentation and increased during the fermentation carried out with Saccharomyces cerevisiae SafSpirit HG-1. In general, the application of keeving contributed to a decrease in the concentration of ethyl and methyl esters, but caused an increase in the concentration of all acetate esters and terpenoids. When the amount of nutrients in the environment is limited and starvation occurs, microorganisms use the available nutrients for basic metabolic processes that allow them to survive and limit the formation of side metabolites such as volatiles. However, most of the samples fermented after the faecal depletion achieved high scores for the floral, fruity, and “overall note” parameters in the sensory analysis. This means that this method, carried out with a properly selected yeast strain, could be feasible for the distilling industry.

Is malting an absolute must? Native triticale as a stand-in for barley malt in the brewing process.

To remain competitive, brewers must innovate by incorporating novel elements beyond traditional styles. Thus, exploring triticale as a modern substitute for barley malt is promising, especially given its higher amylolytic activity compared to barley. This study aimed to assess the impact of substituting up to 50% of barley malt with unmalted triticale on green beer quality, encompassing multiple stages from wort production to primary fermentation at a laboratory scale. Triticale-based worts (ratios 10-50%) had lower extract content than 100% barley malt. However, incorporating 10% of triticale led to only a 1% decrease in extract content compared to the all-malt wort. Shearzyme® 500L, an endo-1,4-β-xylanase with β-glucanase side activity, effectively addressed wort viscosity by breaking down arabinoxylans and β-glucans in triticale cell walls. All triticale-based beers exhibited lower ethanol content compared to reference beer, as is typical when using adjuncts. In green beer, a 50% triticale ratio lowered ethanol content by 16% (without enzyme) and 19% (with enzyme) compared to 100% malt beer. However, green beer with 10% triticale had satisfactory levels of total polyphenol and vicinal diketone content, among other parameters. Commercial enzyme application significantly enhanced proteolytic activity within the grain. Fermentations of enzyme-treated worts showed higher amino acid levels, further confirming the increased proteolytic activity facilitated by the chosen enzyme. Overall, this study provides a comprehensive analysis of the brewing process using native triticale. Building on this foundation, future studies will focus on optimizing mashing conditions to enhance the fermentation profile of the wort. © 2024 Society of Chemical Industry.

Restoring CFTR function with Orkambi decreases the severity of alcohol-induced acute pancreatitis.

Heavy alcohol intake is one of the most common causes of acute pancreatitis (AP). We have previously shown that ethanol (EtOH) decreases the expression and activity of the cystic fibrosis transmembrane conductance regulator (CFTR), which plays a key role in alcohol-induced AP development. The prescription drug, Orkambi (a combination of ivacaftor and lumacaftor) can correct impaired CFTR function and expression in cystic fibrosis (CF) patients. Thus, the present study aimed to investigate whether Orkambi can mitigate alcohol-induced AP. Intact guinea-pig pancreatic ducts were pre-treated with different concentrations of ethanol (EtOH; 30, 50 and 100mm) for 12h alone or in combination with ivacaftor (VX770) and/or lumacaftor (VX-809), and CFTR expression and activity were evaluated by immunostaining and by the patch clamp technique, respectively. Alcoholic AP was induced in Orkambi-treated guinea-pigs, and standard laboratory and histological parameters were measured. Ivacaftor and lumacaftor alone or in combination dose-dependently restored the apical expression and activity of CFTR after EtOH treatment in vitro. Oral administration of Orkambi reduced the severity of alcohol-induced AP and restored impaired CFTR activity and expression. Orkambi is able to restore the CFTR defect caused by EtOH and decreases the severity of alcohol-induced pancreatitis. This is the first in vivo pre-clinical evidence of Orkambi efficacy in the treatment of alcohol-induced AP. KEY POINTS: Acute pancreatitis is one of the leading causes of hospital admission among gastrointestinal diseases in which the lack of a specific drug therapy plays a crucial role. The cystic fibrosis transmembrane conductance regulator (CFTR) plays an essential role in pancreatic ductal HCO3 - secretion; inappropriate CFTR function, as seen in heavy alcohol consumption, increases the risk of pancreatitis development. CFTR modulators are able to prevent the inhibitory effect of ethanol and reduce pancreatic ductal injury and the severity of alcohol-induced pancreatitis. CFTR modulators present a novel option in the pharmacotherapy of alcohol-induced pancreatitis by enhancing pancreatic functions or preventing recurrence.

Preparation and Characterization of Universal Liquid Proliposomes Encapsulating Water-soluble Efficacious Substances.

Liposomes were extensively used for cosmetics and pharmaceuticals due to their versatility, biocompatibility, and biodegradability, as well as the ability to encapsulate water-soluble and fat-soluble substances. However, some challenges remain unsolved, including poor stability, complex preparation process, limited encapsulation efficiencies (EE%) and drug loading capacity (DLC%). We herein prepared universal liquid proliposomes by rotary evaporation method and optimized formulations with different pH, glycerol content, ethanol content and preparation process. The EE% of water-soluble substances was above 85%, and the maximum DLC% was 37.5%. In 7 different conditions, the optimal formulation of the proliposomes remained stable over 60 days. The excellent stability of proliposomes and nicotinamide liposomes and their essence, when applied to cosmetic formulations, was confirmed by the Turbiscan stability analyzer. The proposed universal liquid proliposomes can form liposomes encapsulating a variety of water-soluble substances rapidly, making them an accessible and versatile tool for improving the stability and applicability of water-soluble raw materials.

Development of an Effective Sterilization Protocol for Plant Tissue Culture Studies in Superfruit Aronia [Aronia melanocarpa (Michaux) Elliot

Effective sterilization protocols are crucial for a successful tissue culture study in Aronia. These protocols directly influence contamination rates, shoot health, and root development. In this context, the aim of the study is to develop an effective sterilization protocol for plant tissue culture studies in Aronia [Aronia melanocarpa (Michaux) Elliot], commonly known as the "superfruit." In study, shoot tips of the Nero Aronia variety were used as material. The sterilized shoot tips were transferred to the respective plant tissue culture media in a randomized parcels trial pattern with three replicates, each containing three explants per replicate. Various concentrations and combinations of sterilizing agents, such as sodium hypochlorite (NaOCl), hydrogen peroxide (H2O2), mercuric chloride (HgCl2), and ethanol (C2H5OH), were evaluated to determine their effectiveness in maintaining tissue health and reducing contamination. A total of twelve protocols were developed, incorporating different concentrations of these chemicals. The results showed that the combination of 5% NaOCl and 3% H2O2 provided the lowest average contamination rate (5%) and the highest average number of healthy (uncontaminated) explants (9.00 piece), demonstrating the sterilization efficiency of this combination. On the other hand, protocols containing HgCl2, especially at higher concentrations, resulted in impaired root development. High ethanol concentrations also contributed to effective sterilization, with the combination of 7% NaOCl and 80% ethanol yielding a low contamination rate (8%) and preserving tissue health. This study emphasizes the need to balance sterilization protocols between effective contamination control and tissue viability. The findings are expected to benefit the improvement and development of tissue culture techniques for Aronia and similar species, providing a basis for further research on effective sterilization practices, which are currently limited in Aronia tissue culture.

Optimizing polyphenol extraction and UPLC-MS/MS analysis from red clover (Trifolium pratense L.) species using response surface methodology

Polyphenols were extracted from red clover (Trifolium pratense L.) plants following conventional extraction. The combined factors of ethanol concentration (60–80%), extraction time (15–45 min) and extraction temperature (40–80°C) were evaluated. Central composite design software was used to conduct Response Surface Methodology (RSM) modelling and optimize the polyphenol yield from the red clover. Total phenolic content, antioxidant activity and ultra-performance liquid chromatography mass-spectrometry (UPLC-MS/MS) analysis were used to quantify polyphenols. The RSM model identified ethanolic composition having greatest influence (p < 0.05) on the polyphenol yield with extraction yields increasing with percentage ethanol. The optimal extraction conditions were identified as 80% ethanol for 45 minutes at 40°C. When compared with an exhaustive polyphenol extraction method (70% ethanol for 21 h at room temperature), the optimal extraction resulted in a 25.6% increase in TPC yield. UPLC-MS/MS was performed to quantify the individual polyphenols present within the sample, in which biochanin A and formononetin were present in abundance. The outcomes of this modelling and optimization study demonstrate that polyphenols from red clover can be extracted efficiently with minimal environmental and energy cost and thus had the capability to serve as a source of valuable nutraceutical and pharmaceutical products.

Selenium Attenuates Ethanol-induced Hepatocellular Injury by Regulating Ferroptosis and Apoptosis.

Ferroptosis is a newly identified type of cell death which is strongly linked to the development of several diseases. Whereas, the role of ferroptosis in the improvement of ethanol-induced hepatocytes injury by selenium has not been confirmed. In this study, an in vitro cell damage model was established using half inhibition concentration of ethanol to induce NCTC clone 1469. Cell activity, lipid peroxidation, apoptosis and the expression of markers related to ferroptosis pathway was determined. A mouse model of alcoholic liver disease (ALD) was constructed and the effectiveness of selenium and ferrostatin-1 in treating ALD in vivo was assessed by serum liver function tests, tissue staining and immunohistochemistry for ferroptosis related proteins. Pretreatment with selenomethionine and ebselen significantly improved ethanol-induced reduction in hepatocyte viability, elevated GSH levels and SOD enzyme activity, reduced MDA and iron content, while improving ethanol-induced changes in apoptosis levels and ferroptosis markers GPX4, SLC7A11, and ACSL4, with the effect of Selenomethionine being more significant. In vivo results also indicated that intervention with selenium or ferroptosis inhibitors significantly improved ethanol-induced liver tissue damage, significantly reduced serum ALT and AST levels, upregulated GPX4 and SLC7A11, but reduced ACSL4 protein levels in liver tissue. The process of ethanol damage to hepatocytes is regulated by the ferroptosis pathway. Selenium may exert a beneficial role in ethanol-induced hepatocyte injury by antagonizing oxidative stress and regulating apoptosis and ferroptosis pathways.

Ethanol Production Using Zymomonas mobilis and In Situ Extraction in a Capillary Microreactor

The bacterium Zymomonas mobilis is investigated as a model organism for the cultivation and separation of ethanol as a product by in situ extraction in continuous flow microreactors. The considered microreactor is the Coiled Flow Inverter (CFI), which consists of a capillary coiled onto a support structure. Like other microreactors, the CFI benefits from a high surface-to-volume ratio, which enhances mass and heat transfer. Compared to many other microreactors, the CFI offers the advantage of operating without internal structures, which are often used to ensure good mixing. The simplicity of the design makes the CFI particularly suitable for biochemical applications as cells do not get stuck or damaged by internal structures. Despite this simplicity, good mixing is achieved through flow vortices caused by Taylor and Dean vortices. The reaction system consists of two phases, in which the aqueous phase carries the bacterium and an oleyl alcohol phase is used to extract the ethanol produced. Key parameters for evaluation are bacteria growth and the amount of ethanol produced by the microorganism. The results show the suitability of the CFI for microbial production of valuable compounds. A maximum ethanol concentration of 1.26 g L−1 was achieved for the experiment in the CFI. Overall, the cultivation in the CFI led to faster growth of Z. mobilis, resulting in 25% higher ethanol production than in conducted batch experiments.

Solubility effect of deep eutectic solvent and ethanol concentration on corncob lignin extraction

This research investigates the solubility effect of deep eutectic solvent (DES) mass ratio and ethanol–water solution concentration on corncob lignin extraction. The objective is to propose a solubility matching technique based on like-dissolves-like theory to optimize lignin extraction conditions. The choline chloride (ChCl):lactic acid (LA) mass ratio that maximized lignin extraction was determined by varying DES mass ratio between 1:2 and 1:5, and ethanol–water solution (5–95 % (v/v)) was added to precipitate lignin. By using the solubility matching technique, the maximum lignin extraction was achieved at 1:4 ChCl:LA mass ratio, and 70 % (v/v) ethanol–water concentration yielded the highest lignin precipitation. Lignin is highly soluble where the solubility (δ-values) of lignin, DES, and precipitant are similar. Unlike conventional trial-and-error methods, the solubility matching technique relies on δ-values calculated by the Hildebrand and Scott equation. The proposed technique can also be applied to optimize the extraction conditions of other biomass materials.

Zinc oxide-based sensor prepared by modified sol–gel route for detection of low concentrations of ethanol, methanol, acetone, and formaldehyde

Abstract We4 4 We received the acceptance date as 26 September 2024. Could you please update it to reflect this exact date for our project, instead of 1 October 2024? This is very important for our project funding. successfully synthesized zinc oxide (ZnO) nanoparticles using the sol–gel method, followed by their application onto alumina substrates for sensor testing. Comprehensive characterization of the nanomaterials was carried out utilizing XRD, SEM, TEM, UV–VIS-IR, and Photoluminescence (PL) techniques. The nanoparticles displayed a hexagonal wurtzite crystal structure, typical of ZnO. UV–Vis-IR spectroscopy revealed significant absorption in the UV region, with the band gap energy calculated to be 3.22 eV. PL spectra indicated the presence of various defects, such as oxygen vacancies and zinc interstitials, within the ZnO structure. SEM analysis of the deposited film surface showed spherical agglomerates, confirming the nanoscale dimensions, while energy-dispersive x-ray spectroscopy spectra affirmed the high purity of the ZnO films, rich in Zn and O elements. Sensor tests demonstrated the ZnO sensor’s high sensitivity to low concentrations of volatile organic compounds such as ethanol, formaldehyde, methanol, and acetone. Notably, at an operational temperature of 300 °C, the sensor exhibited a remarkable response to 5 ppm of each gas, with the following response and response/recovery times: for methanol, 11.47 and 36 s/57 s; for acetone, 11.54 and 25 s/52 s; for formaldehyde, 0.79 and 53 s/58 s; and for ethanol, 3.88 and 9 s/59 s.

Orange peel: Low cost agro-waste for the extraction of polyphenols by statistical approach and biological activities

Food waste is an excellent source of various bioactive secondary metabolites. In addition, food waste is a global issue, and more research is being focused on the conversion of value-adding products from food waste, especially fruits. In this study, bioactive polyphenols were extracted from orange peels. The parameters that affect the extraction of polyphenols from orange peels were optimized using response surface methodology. Ethanol was used to extract bioactive polyphenols, and the extraction time (min), extraction temperature, and ethanol concentration (%) were analyzed. Orange peels are an excellent source of polyphenols, and the yield was increased under optimized extraction conditions (p&lt;0.05). The polyphenol content increased twofold (38.4 mg GAE/g) under the optimized extraction conditions (53% ethanol, 51 degrees centigrade and 96 min extraction time). Crude polyphenols exhibited significant antibacterial activity against Bacillus subtilis (19±1 mm zone of inhibition), followed by Staphylococcus aureus (17±2 mm zone of inhibition) (p&lt;0.05). In addition, orange peel extract exhibited antifungal activity against A. flavus and C. albicans. The ethanol extract exhibited DPPH activity (1.61±0.03 mg GAE/100 g) and ABTS reducing power (1.48±0.01 mg GAE/100 g). The polyphenol compounds exhibited antibiofilm activity against biofilm-producing Bacillus subtilis and increased in a dose-dependent manner (p&lt;0.05).

Computational study on the impact of gasoline-ethanol blending on autoignition and soot/NOx emissions under low-load gasoline compression ignition conditions

In the present work, computational fluid dynamics (CFD) simulations of a single-cylinder gasoline compression ignition (GCI) engine are performed to investigate the impact of gasoline-ethanol blending on autoignition, nitrogen oxide (NOx), and soot emissions under low-load conditions. In order to represent the test gasoline (RD5-87), a four-component toluene primary reference fuel (TPRF)+ethanol (ETPRF) surrogate (with 10% ethanol by volume; E10) is employed. A three-dimensional (3D) engine CFD model employing finite-rate chemistry with a skeletal kinetic mechanism (including NOx sub-mechanism), adaptive mesh refinement (AMR), and hybrid method of moments (HMOM) is adopted to capture the in-cylinder combustion phenomena and soot/NOx emissions. The engine CFD model is validated against experimental data for three gasoline-ethanol blends: E10, E30 and E100, with varying ethanol content by volume. Model validation is carried out for a broad range of start-of-injection (SOI) timings (−21, −27, −36, and −45 crank angle degrees (°CA) after top-dead-center (aTDC)) with respect to in-cylinder pressure, heat release rate, combustion phasing, NOx and soot emissions. For relatively later injection timings (−21 and −27 °CA aTDC), E30 yields higher amount of soot than E10; while the trend reverses for early injection cases (−36 and −45 °CA aTDC ). On the other hand, E100 yields the lowest amount of soot among all fuels irrespective of SOI timing. Further, E10 shows a non-monotonic trend in soot emissions with SOI timing: SOI-36&gt;SOI-45&gt;SOI-21&gt;SOI-27, while soot emissions from E30 exhibit monotonic decrease with advancing SOI timing. NOx emissions from various fuels follow a trend of E10&gt;E30&gt;E100. On the other hand, NOx emissions increase as SOI timing is advanced for all fuels, with an anomaly for E10 and E100 where NOx decreases when SOI is advanced beyond −36 °CA aTDC. Detailed analysis of the numerical results is performed to investigate the soot/NOx emission trends and elucidate the impact of chemical composition and physical properties on autoignition and emissions characteristics.

Polyphenol composition and antioxidant activity of fermentation combined with enzymatic hydrolysis modified Astragalus membranaceus stems

Astragalus membranaceus (AM) roots are a well-known homologous medicine and food in China. AM stems, which are discarded and not used effectively, also contain many active compounds and exhibit beneficial effects. It has the potential to be explored as antibiotic alternative. Fermentation combined with enzymatic hydrolysis (FEH) is an effective strategy for extracting polyphenol and improving the usage of AM stems. Therefore, in this study, the conditions of FEH and extraction for polyphenol in AM stems were screened. The antioxidant activity of extract from AM stems without and with FEH (AMSE and FAMSE) was evaluated. The metabolite profiles of phenolic acids and flavonoids in AMSE and FAMSE were characterized by ultra-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC–ESI-MS/MS). The results showed that the highest polyphenol content from AM stems was obtained with cellulase and pectinase (1:1, 2000 U/g), moisture content 43%, fermentation temperature 30 °C, and fermentation time 7 days. Selected extraction conditions of polyphenol were ethanol concentration 50%, ultrasonic power 500 W, extraction temperature 35 °C, and extraction time 40 min. On this condition, compared with AMSE, the polyphenol and flavonoid contents in FAMSE were significantly higher. FAMSE exhibited stronger DPPH, hydroxyl radical scavenging rate and reducing power than AMSE. The relative content of 3-(4-hydroxyphenyl)-propionic acid, dihydroferulic acid, isoferulic acid, 4-hydroxybenzoic acid, 4-hydroxyphenyllactic acid, ferulic acid, vanillic acid, syringic acid, gentisic acid, sinapic acid, apigenin, tricin, acacetin, daidzein, genistein, formononetin, prunetin, pratensein, rhamnocitrin and galangin were significantly upregulated in FAMSE.Graphical

Optimasi Metode Microwave-Assisted Extraction (MAE) untuk Menentukan Kadar Flavonoid Total Ekstrak Etanol Kulit Pisang Kepok (Musa Paradisiaca.L)

Indonesia is a country that has a lot of banana diversity. One of the banana plants that is widely found in Indonesia is the Kepok banana (Musa Paradisiaca.L). Kepok banana skin has a very high content of flavonoids and phenols. Flavonoids are known as good antioxidants because they can find reactive oxygen species with the phenolic hydroxyl group that flavonoids have. The aim of this research was to determine the results of the optimization of the Microwave-Assisted Extraction (MAE) method on the total flavonoid content of kapok banana peel ethanol extract. Microwave-Assisted Extraction (MAE) was done to extraction flavonoid from ethanol extract of kepok banana peel (Musa Paradisiaca.L). The sample collected from Widoro village, Karangsambung sub-district, Kebumen regency. Optimum extraction condition was determined by the Response Surface Methodology (RSM). The Box- Behnken design (BBD) was used to evaluate the influence of 3 factors with 3 levels extraction that is power level (10%, 30%, 50%), ethanol concentration (50, 70, 96%), and extraction time (3, 5 and 7 minutes) with 15 different runs. The research showed that optimum extraction condition was at 24,14 % of power level, 66,26% ethanol concentration, and time extraction of 3,52 minutes with calculations from the Design Expert program, the total flavonoid content response was 8.491 Mg QE/g. The total flavonoid content value obtained experimentally was 8,246 Mg QE/g under condition of 30 % power level, 70% ethanol concentration, and time extraction of 5 minutes. Based on the result, total flavonoid content can be significantly increased by optimizing the MAE process use RSM.

Enhanced bioethanol production from cassava waste: optimization of thermochemical pretreatment for maximum sugar recovery and characterisation of potential fractions

ABSTRACT Thermochemical pretreatment is an interesting approach for the effective separation of lignocellulosic biomass before fermentation for bioethanol production. This research focused on optimising the thermochemical pretreatment conditions of cassava waste using response surface methodology to predict the glucose yields under the optimal condition. The optimised conditions of 0.045 M of NaOH concentration at 153.59 °C for 48.03 min resulted in the maximum glucose yield of 93.87%. In addition, fermentation process can generate ethanol within a range of solid loading from 10% to 20%. The highest concentration of ethanol was 34.53 ± 0.56 g/L after 72 h, equivalent to ethanol yield of 0.46 g/g. The co-products were also characterised for further utilisation in biorefinery concept. The results of this study confirmed that the thermochemical pretreatment process using the NaOH catalyst could provide high glucose yields, which can be easily converted into bio-based fuels and further valorised of co-products to value-added chemicals.