Contamination Of Fresh Vegetables Research Articles

Papaya Seed Extract Combined Lauryl Glucoside Against Human Parasites Contaminated in Fresh Vegetables

Background: The prevalence of parasites in Thailand, primarily transmitted through contaminated vegetables, poses a significant public health concern. Ensuring the safety of natural produce from both parasites and chemical residues necessitates robust sanitation measures. Objective: This study aims to develop effective vegetable-washing solutions using papaya seed extract with potent anti-parasitic properties contamination in fresh vegetables. Methods: Fresh vegetables contaminated with Strongyloides stercoralis larvae, Ascaris lumbricoides, and Taenia spp. eggs were subjected to various treatments: distilled water, tap water, vinegar (5%), papaya seeds extract (200 mg/L), lauryl glucoside (1%) from coconut oil, papaya seeds extract combined with lauryl glucoside, and Albendazole as a standard drug. Immersion for 5 minutes was followed by parasite observation using the sedimentation method over 60 minutes, examining inhibitory effects on parasite larvae movement. Results: Papaya seed extract achieved the most rapid cessation within 10 minutes for larvae movement, followed by vinegar and papaya seed extract combined with lauryl glucoside. Furthermore, papaya seed extract combined with lauryl glucoside exhibited the highest efficacy in the sedimentation larval and parasite eggs, followed by lauryl glucoside solution and papaya seed extract, surpassing the control group. Conclusion: Papaya seeds extract immobilized larvae, while lauryl glucoside effectively eliminated parasite eggs from vegetables. Natural and organic compounds derived from papaya and coconut oil exhibit inherent diversification in their properties. These compounds originate from natural sources, showcasing distinct characteristics attributed to their organic nature. These results indicate the potential for developing natural extract-based products as future vegetable-washing solutions, emphasizing their significance against parasitic efficacy.

Toxoplasma gondii contamination in fresh vegetables destined for human and livestock consumption in Thua Thien Hue, Vietnam

Toxoplasmosis caused by Toxoplasma gondii is an important zoonosis that shows severe symptoms in immunocompromised patients. High seroprevalences of toxoplasmosis were found in cats, humans, and pigs in traditional farms in Thua Thien Hue province. The main sources of infections remain unknown, thus making toxoplasmosis neglected and uncontrollable in this region. This study aimed to determine T. gondii contamination in vegetables used for human and livestock consumption and its spatial distribution in Thua Thien Hue rural areas. A cross-sectional study was conducted to investigate T. gondii contamination in vegetables grown in households in three different geographic regions. The pathogen DNA was detected from vegetable samples using a primer pair that is highly specific for the 529-bp repetitive element found in T. gondii genome. In this study, 55 out of 221 (24.9%) vegetable samples were positive for T. gondii using PCR. Factors, including location, presence of fence in the garden, owning cats, and treatment of feed for cats, were not significantly associated with T. gondii contamination in vegetables according to the logistic regression. However, vegetables collected in households that have no fence for vegetable gardens, own cats, and feed cats raw or undercooked foods tend to be more heavily contaminated than others. Furthermore, a wide distribution of infected vegetables was observed in all investigated districts, showing a high infection pressure in these residential areas. This is the first report of vegetables contaminated with T. gondii in Vietnam, which reveals an overlooked health risk for humans and animals.

Faktor risiko kontaminasi telur cacing soil transmitted helminths pada sayur lalapan

Management process of fresh vegetables can indicate the incidence of Soil Transmitted Helminths (STH) infection in the community.The purpose of this study was to determine the risk factors that influence STH contamination in fresh vegetables from food stalls in Pedurungan District, Semarang. This type of research is descriptive using qualitative methods with cross sectional design. Based on the results of laboratory tests, a total of 165 samples examined found STH worm eggs with the species Trichuris trichiura (0.6%). Based on bivariate analysis, there was no significant relationship between education level, knowledge, personal hygiene and environmental sanitation to contamination of STH worm eggs in fresh vegetables in Semarang City.

Soil-Transmitted Helminth Eggs Contamination on Fresh Vegetables in Medan Indonesia

Indonesian people habitually consume fresh vegetables as companions to other foods. We can find soil-transmitted helminths (STH) eggs in fresh vegetables that are not washed clean. This study determines whether there was STH egg contamination in fresh vegetables in several stalls selling fried chicken in Medan. This study was a descriptive study with a cross-sectional design. Research samples were four types of fresh vegetables: lemon basil (Ocimum africanum), lettuce (Lactuca sativa), cucumber (Cucumis sativus), and cabbage (Brassica oleracea var. capitata) collected from 88 fried chicken stalls in Medan in January 2019. All samples were examined by the sedimentation method to ensure STH egg contamination at the Parasitology Laboratory of the Faculty of Medicine Universitas Muhammadiyah Sumatera Utara. The types of fresh vegetables that were most contaminated by STH eggs were lemon basil leaves (Ocimum africanum), and the most abundant STH eggs found were Ascaris lumbricoideseggs. In conclusion, there is egg contamination in fresh vegetables in several stalls selling fried chicken in Medan.

Contamination of fresh vegetables in municipal stores with pathogenic Acanthamoeba genotypes; a public health concern

ABSTRACT Acanthamoeba spp. cause keratitis and encephalitis, and are a proper carrier of foodborne pathogens. A total of 70 samples including garden cress, chives, mint, parsley, and basil were collected. Samples were cultured onto a 2% non-nutrient agar medium. The cultures were analyzed using morphological and molecular techniques. In total, 18 (25.7%) out of 70 samples were positive including garden cress 10/22 (45.45%), chives 3/12 (25%), mint 2/13 (15.38%), basil 2/13 (15.38%), and parsley 1/10 (10%). The diagnostic fragment 3 was successfully sequenced in 15 samples and represented 11 (73.3%) T4, three (20%) T5, and one T9 genotypes. In addition, three, two, and one strains, belonging to the genotypes T4, T5, and T9 were ranked highly pathogenic. This is the first study reporting contamination of the most commonly consumed fresh vegetables with pathogenic Acanthamoeba genotypes. Our findings signify the public health concerns due the contamination of vegetables in municipal public markets.

Parasitic contamination of fresh vegetables sold in open markets: a public health threat

Abstract Vegetables eating raw are a leading source of transmission of infective forms of pathogenic internal parasites among human beings. This research was conducted from April to October, 2017 to assess the parasitic contamination of vegetables sold at main vegetable markets in districts Lower Dir and Peshawar, Pakistan. Eight hundred specimens of different vegetables were purchased and soaked in physiological saline solution, shaken with a mechanical shaker for 20 minutes and processed by sedimentation concentration method. Results revealed that only 19.7% (n=158/800) of the vegetables were found to be contaminated with single or multiple parasite species. Ascaris lumbricoides (the large round worm) 12.3% (n=99/800) was the most commonly detected pathogen and Taenia saginata (the beef tapeworm) 1.62% (n=13/800) was the least frequently detected one. Interestingly, significant p value (p>0.05 at 95%CI) between the number of examined and contaminated for all the variables studied including education status of the vendors, markets location, type of vegetables, means of display, washed before display, washing source of water and market type. The findings of this study evidenced that consumption of raw vegetables possesses great risk of getting parasitic infections in Lower Dir and Peshawar districts, Pakistan. Instructing the sellers and the public about parasitic disease transfer and their hygiene can reduce the infection rate of parasites of human origin.

Contamination of Fresh Vegetables with Protozoan Parasites, in Duhok City, Kurdistan Region of Iraq

Background: Vegetables are essential for the human body and they constitute the main daily consumed diet. However, vegetable consumption is one of the main routes of transmission of intestinal parasites in humans. Objective: This study aimed to investigate parasites that caused vegetable contamination in Duhok city, Kurdistan region of Iraq. Materials and Methods: In the current study, from June 2021 through August 2021, a total of 166 samples of fresh vegetables were collected from several markets in different places of Duhok city. Samples were examined microscopically for parasitic investigation. Results: It was shown that 10.2% of vegetables were contaminated with parasites, but with no significant margin between contaminated and non-contaminated vegetables (P value = 0.81). The parasitic contamination was detected in lettuce (15%), parsley (14.3%), and radish (14.3%), with no parasitic contamination in both carrot and cucumber. Three types of parasites were detected in vegetable samples; they were in the order of 5.4% for Entamoeba spp., 4.2% Giardia lamblia, and 1.2% Balantidium coli. Conclusion: In conclusion, the present study identified fresh vegetable contamination with protozoan parasites in moderate rate, which can play an important role in the transmission of pathogenic intestinal parasites.

Parasitic Contamination of Common Vegetables Sold in Lusaka, Zambia

Background: Consumption of raw or unhygienically prepared vegetables is a potential source of parasitic infection. This study aimed to establish the prevalence and types of intestinal parasites on the freshly sold vegetables at the market. Methods: Totally, 95 vegetable samples were randomly procured from vendors at Lusaka City market (Zambia). The samples were examined for parasitic contamination using floatation and sedimentation methods. Data were analyzed using Epi Info version 7.2.4.0. Results: The overall parasitic contamination of fresh vegetables was 35.8% (34 out of 95). The highest parasitic contamination rate was found in Chinese cabbage with 7.4% (7 out of 95), followed by rape with 6.3% (6 out of 95), chilli with 5.3% (5 out of 95), pigweed with 5.3% (5 out of 95), pumpkin leaves with 5.3% (5 out of 95), tomatoes with 4.2% (4 out of 95), and cabbage with 2.1% (2 out of 95). There was a significant (p=0.001) statistically difference between the type of vegetables and the presence of the parasites. The identified helminths had different prevalence rates, including Taenia spp. with 9.5%, Trichuris trichuria with 5.3%, Ascaris lumbricoides with 3.2%, Clonorchis sinensis with 2.1%, Diphyllobothrium latum with 2.1%, Paragonimus westermani with 2.1%, and Strongyloides stercoralis with 2.1%. Also, Giardia lamblia with 24.2% and Balantidium coli with 4.2% were the protozoan parasites identified. Conclusion: This study showed that some vegetables sold at the Lusaka City market (Zambia) were a potential source of parasitic infections for local consumers. G. lamblia was the most prevalent parasite found in the vegetable samples.

Diversity and antimicrobial drug resistance of non-typhoid Salmonella serotypes isolated in lettuce, irrigation water and clinical samples in Burkina Faso

The contamination of fresh vegetables by non-typhoid Salmonella species present in irrigation water and animal feces could causes of many diarrheal infections and diseases. The aim of this study was to determine the diversity of non-typhoidal Salmonella serotypes and their antimicrobial resistance profile from lettuce, irrigation water and human samples. This study concern 57 Salmonella isolated from 134 lettuce and irrigation water samples and 416 stool samples. All Salmonella isolated were characterized used serotyping antisera according to Kauffman-White-Le Minor scheme. Salmonella strains were characterized by simplex PCR using specific primers. Out of 57 Salmonella isolated, (42/134) 31.34% were from lettuce and irrigation water and (15/416) 03.6% from human. A total of 36 different serotypes were identified. Highest prevalence was obtained with S. Cubana, S . Jodhpur and S . Kentucky 05/57 (08.77% each) followed by S . Poona 04/57 (07%). Resistance genes CTX-M1, aadA1, dfrAI, were obtained with S. Jodhpur and CTX-M2, aac(3-Id), aadA7 with S. Kentucky. However, resistance genes gyrA , gyrB and parE were found in S . Essen, S. Poona and S . Senftenberg. Virulence gene such as invA , pipD and misL were found in 91% globally, followed by orfL 81% and spvR 12%. This study highlighted the diversity of non-typhoid Salmonella serotypes and resistance genes circulating in environment and hospital center in BF. Gardening products should be a potential source of transmission of Salmonella causing diarrhea among human in BF. Microbiological and antimicrobial resistance plans must be implemented so that they are tools for infection prevention. • Non-typhoidal Salmonella serotypes in lettuce, irrigation water and clinical samples. • Diversity of resistance genes circulating in environment and hospital center. • Gardening products, potential source of transmission of Salmonella causing diarrhea.

Bacillus cereus Isolated From Vegetables in China: Incidence, Genetic Diversity, Virulence Genes, and Antimicrobial Resistance.

Bacillus cereus is a food-borne opportunistic pathogen that can induce diarrheal and emetic symptoms. It is widely distributed in different environments and can be found in various foods, including fresh vegetables. As their popularity grows worldwide, the risk of bacterial contamination in fresh vegetables should be fully evaluated, particularly in vegetables that are consumed raw or processed minimally, which are not commonly sterilized by enough heat treatment. Thereby, it is necessary to perform potential risk evaluation of B. cereus in vegetables. In this study, 294 B. cereus strains were isolated from vegetables in different cities in China to analyze incidence, genetic polymorphism, presence of virulence genes, and antimicrobial resistance. B. cereus was detected in 50% of all the samples, and 21/211 (9.95%) of all the samples had contamination levels of more than 1,100 MPN/g. Virulence gene detection revealed that 95 and 82% of the isolates harbored nheABC and hblACD gene clusters, respectively. Additionally, 87% of the isolates harbored cytK gene, and 3% of the isolates possessed cesB. Most strains were resistant to rifampicin and β-lactam antimicrobials but were sensitive to imipenem, gentamicin, ciprofloxacin, kanamycin, telithromycin, ciprofloxacin, and chloramphenicol. In addition, more than 95.6% of the isolates displayed resistance to three kinds of antibiotics. Based on multilocus sequence typing, all strains were classified into 210 different sequence types (STs), of which 145 isolates were assigned to 137 new STs. The most prevalent ST was ST770, but it included only eight isolates. Taken together, our research provides the first reference for the incidence and characteristics of B. cereus in vegetables collected throughout China, indicating a potential hazard of B. cereus when consuming vegetables without proper handling.

Prevalence of Parasites of Public Health Significance in Vegetables Sold in Jos Metropolis, Plateau State, Nigeria

BACKGROUND: Vegetables are vital for good health but can serve as a means of transmission of parasitic pathogens to man. JUSTIFICATION: There is increase consumption of vegetables due to general awareness of the health benefits. This research intends to explore suggestive management strategies and control in handling vegetables to reduce or if possible eliminate food borne parasites. AIM AND OBJECTIVE: The study was to determine parasitic contamination of fresh vegetables with objective to determine the prevalence of parasites of medical importance in vegetables sold in Jos market. METHODOLOGY: A total of 575 samples of fresh vegetables; Lettuce, Cabbage, Carrot, Spinach, Green Pepper, Cucumber, Beetroot, Tomatoes, Garden egg, and Green bean; were bought from sellers from May-June 2016 and examined for helminthes eggs, larva and cysts using wet mount and concentration techniques. RESULT: Hookworm, Ascaris lumbricoides, Trichuris trichuria, Strongyloides stercoralis, cyst of Entamoeba histolytica and Giardia lambia accounted for 7.3%, 8.1%, 4.8%, 58.1%, 9.7% and 12.1% respectively. Cucumber, carrot, cabbage beetroot, spinach, lettuce, green pepper, green beans, garden egg and tomatoes had 0%, 26.7%, 31.7%, 5.7%, 40%, 53.3%, 6.7%, 6.7%, 13.3% and 25% respectively with overall prevalence of 21.6% and strongyloides stercoralis was most prevalent and Trichuris trichiura least prevalent found in cabbage and tomatoes. Lettuce has the highest contamination rate 32(53.3%), followed by spinach 24(40%) while beetroot had the least (5.7%). CONCLUSION: Vegetables in Jos Metropolis are contaminated with parasites. There should be proper washing as they could serve as source of transmission of parasites when eaten raw or undercooked.

<p>Fruit and vegetable contamination with medically important helminths and protozoans in Tarcha town, Dawuro zone, South West Ethiopia</p>

Background: Fresh vegetables supply the body with essential supplements. The consumption of raw vegetables and fruits are among the ways for transmission of intestinal parasitic organisms to human beings. This study was aimed at detecting the parasitic contamination in fresh vegetables sold at the central open-aired market of Tarcha town, Ethiopia.Methods: A total of 270 fresh vegetables and fruit samples were collected from the main market of Tarcha town during August 1, 2017–August 22, 2017. The samples were microscopically examined for detection of medically important parasites after washing with 500 mL normal saline and 24-hr sedimentation of the washing solution followed by centrifuging at 2000 g for 5 mins. After centrifugation, the supernatant was decanted leaving the sediment. Finally, the sediment was examined under a light microscope for protozoans and helminths worms.Results: Of the 270 fresh vegetable and fruit samples, 115 (42.6%) were found positive for intestinal parasites. A high level of contamination in fresh vegetable and fruit samples was recorded in cabbage 71.1% (32/45) while tomato was the least contaminated 24.4% (11/45). The identified medically important parasites were Entamoeba histolytica/dispar, Giardia intestinalis, Ascaris lumbricoides, Hymenolepis nana, Toxocara spp, Hymenolepis diminuta, and Cystoisospora belli. The most predominant parasite encountered was Ascaris lumbricoides (16.7%) whereas Cystoisospora belli (2.6%) the least detected. All of the vegetables and fruits that were contaminated were with more than one parasite species. A statistically significant association between the type of vegetables and the presence of parasites was also observed (p=0.002).Conclusion: This study has shown that fruits and vegetables which are sold in the study area are highly contaminated with medically important parasites. Fruits and vegetables sold in the study area may play a role in the transmission of intestinal parasitic infections to humans. Effective and comprehensive prevention measures should be taken to ensure food safety. Relevant bodies should work on addressing the issue of high rate of vegetable and fruit contamination in the study area.

Foodborne Viruses Detected Sporadically in the Fresh Produce and Its Production Environment in South Korea.

Contamination of fresh vegetables and berries with human enteric viruses is a major cause of food poisoning. The aim of this study was to investigate the prevalence of norovirus GI, norovirus GII, hepatitis A virus (HAV), adenovirus, astrovirus, rotavirus, and male-specific coliphage systematically in fresh fruit and vegetables and associated agricultural environmental samples, including irrigation water, soil, and worker's gloves. Enteric viruses were detected by international standard methods (ISO/TS 15216), and male-specific coliphages were isolated using US EPA Method 1601. For the study, 773 samples were collected from June 2016 to April 2017, including Chinese cabbage (n = 244), cucumber (n = 98), lettuce (n = 73), strawberry (n = 120), soil (n = 191), irrigation water (n = 14), and gloves (n = 27). Two cucumber and two irrigation water samples were positive for norovirus GI, and one cucumber and two irrigation water samples were positive for norovirus GII. HAV was detected in one strawberry sample and one glove sample. The other tested foodborne viruses were not detected in any of the samples. Sixteen male-specific coliphages were isolated from Chinese cabbage, cucumber, lettuce, cherry tomato, soil, and irrigation water. The isolation of male-specific coliphage would be more practical to investigate the fecal contamination in produce rather than pathogenic viruses.

Efficacy of Combination Treatment with Sodium Metasilicate and Sodium Hypochlorite for Inactivation of Norovirus on Fresh Vegetables.

In recent years, fresh vegetables have frequently been associated with the foodborne transmission of enteric viruses, such as human norovirus (NoV). Therefore, several studies have focused on developing methods to inactivate foodborne viruses for preventing outbreaks of foodborne illnesses. Sodium hypochlorite (NaOCl) is commonly used as a disinfectant, but results in undesirable effects on the appearance and taste of foods and can generate toxic byproducts when it exceeds the allowable concentration. Here, we evaluated the efficacy of a range of NaOCl concentrations (50-1000 ppm) for reducing the amounts of human NoV (NoV GII.4) on lettuce (Lactuca sativa), celery (Apium graveolens L.), and white cabbage (Brassica oleracea ssp. capitata). In addition, the combination treatment of NaOCl and sodium metasilicate (SMS, 0.1-0.5%) pentahydrate was evaluated for its ability to decrease the populations of NoV GII.4 in the three food samples. An immunomagnetic separation procedure combined with reverse transcription quantitative polymerase chain reaction was used for virus detection. For lettuce, celery, and cabbage, the NoV GII.4 recovery rates were 57.3% ± 6.5%, 52.5% ± 1.7%, and 60.3% ± 3.9%, respectively, using a glycine/NaCl elution buffer (0.25 M glycine/0.14 M NaCl, pH 9.5). The reductions of NoV GII.4 were 3.17, 3.06, and 3.27 log10 genomic copies/μL for lettuce, celery, and cabbage, respectively, at 1000 ppm NaOCl, while a reduction of ∼3 log10 genomic copies/μL was obtained when the samples were treated with a combination of 100 ppm NaOCl and 0.4% SMS pentahydrate. Taken together, these results demonstrated that combined treatment with NaOCl and SMS pentahydrate was an efficient strategy to reduce the concentration of NaOCl for control of NoV GII.4 contamination in fresh vegetables.

Parasitic contamination of fresh vegetables sold at central markets in Khartoum state, Sudan.

BackgroundFresh vegetables are considered as vital nutrients of a healthy diet as they supply the body with essential supplements. The consumption of raw vegetables is the main way for transmission of intestinal parasitic organisms. This study was aimed at detecting the parasitic contamination in fresh vegetables sold in two central open-aired markets in Khartoum state, Sudan.MethodsIn this prospective cross-sectional study, a total of 260 fresh vegetable samples and 50 water samples used to sprinkle vegetable(s) were collected from two central open-aired markets (namely; Elshaabi and Central markets) during November 2011 to May 2012. The samples were microscopically examined for detection of parasitic life forms using standardized parasitological techniques for protozoans and helminthes worms.ResultsOf the 260 fresh vegetable samples, 35 (13.5 %) were microscopically positive for intestinal parasites whereas 7/50 (14 %) of water samples used to sprinkle vegetable(s) were found positives. Remarkably, high level of contamination in fresh vegetable samples was recorded in lettuce (Lactuca sativa) 36.4 % (4/11) while cayenne pepper (Capsicum annuum) and cucumber (Cucumis sativus) were not contaminated. The identified protozoans and helminthes were Entamoeba histolytica/dispar, Entamoeba coli, Giardia lamblia, Ascaris lumbricoides, Strongyloides stercoralis, T. trichiura and hookworms. The most predominant parasite encountered was E. histolytica/dispar (42.9 %) whereas both T. trichiura and A. lumbricoides (2.9 %) were the least detected parasites. None of the fresh vegetables had single parasitic contamination. The highest percentages found in water samples used to sprinkle vegetable(s) was for Strongyloides larvae 60 % (3/5). It is worth-mentioned that the rate of contamination in Elshaabi market was higher compared with Central market. However, there was no significant correlation between the type of vegetables and existence of parasites in both markets and a high significant relationship was observed between the type of parasite and total prevalence in fresh vegetables (p = 0.000).ConclusionThe study has identified a moderate rate of fresh vegetables contaminated with protozoan and helminthes. Contaminated fresh vegetables in central markets of Khartoum state may play a significant role in transmission of intestinal parasitic infections to humans, and the water used by greengrocers to sprinkle vegetable(s) can be implicated in vegetable contamination.

Survey study of intestinal parasites on Fresh vegetables collected from some Baghdad Sales and its role in human infection

Fresh vegetables are an important part of a healthy diet. The consumption of raw vegetables without cooking or good washing can be a major rout of transmission to the parasitic infection. The goal of this study was to determine the intestinal parasitic contamination of fresh vegetables from vegetables sales markets in Baghdad province during the different above months of the year. A total of 303 samples of different vegetables were randomly selected from three wholesale markets distributed through different regions in Baghdad (East, West and South) and then were examined by a floatation method. The present study showed that the collected vegetables were contaminated with 12 species of intestinal parasites, and the total percentage of contamination was 161 (53.1%) . ZnSo4 solution showed the highest percentage of contaminated vegetables (31.2%) while sucrose showed the lowest percentage (8.9%).

Prevalence of Strongyloides stercoralis contaminating Coriandrum obtained from vendors in Karimnagar

Helminthic contamination of fresh vegetables is common. 116 samples of Coriandrum sativum was processed by Baermann funnel method for recovering Strongyloides stercoralis larvae which were identified by their morphology. The prevalence rate of contamination with larvae was found out. Out of 116 samples, 73 (63%) samples were harboring the parasites and 43 (37%) samples had no larvae. Among 73 positive samples, 25 samples contained rhabdatiform larvae, 12 contained filariform larvae, and 36 contained both types of larvae. 63% prevalence rate of contamination of Coriandrum sativum in Karimnagar is the definite risk factor for acquiring infection by farm workers, handlers, transporters, and consumers of this vegetable. This high prevalence of larvae acts as an environmental source of the parasite; especially the infective filariform forms constituting 65% of the contaminated samples. Larvae can spread from the source through direct contact while handling the vegetable, necessitating estimation of asymptomatically infected persons and burden of the parasitic infestation.

Rapid analysis of U isotopes in vegetables using ICP-MS: application to the emergency U monitoring after the nuclear accident at TEPCO’s Fukushima Dai-ichi power station

After the nuclear accident at TEPCO’s Fukushima Dai-ichi power station in March, hydrogen explosions and reactor building explosion resulted in releases of radionuclides in the environment. Severe radioactive cesium and iodine contaminations have been observed in fallout deposition samples and soils in the East Japan. Radioactive cesium, iodine, uranium, and transuranic radionuclides were set as the monitoring targets in food safety tests. However, so far, only radioactive cesium and iodine were daily measured and reported by the Ministry of Health, Labour, and Welfare. The tedious and time consuming conventional alpha spectrometric method hampered the emergency monitoring U contamination in foods. In this work, we propose a simple and rapid analytical method for 238U and 235U/238U isotope ratio analysis in fresh vegetables. This method was applied to the emergency monitoring of radioactive contamination after the nuclear accident at TEPCO’s Fukushima Dai-ichi power station. The results showed no U contamination in fresh vegetables collected in Chiba and Ibaraki prefectures in April and May, 2011.