Contamination Of Food Products Research Articles

Anti-Biofilm Activity of Cell Free Supernatants of Selected Lactic Acid Bacteria against Listeria monocytogenes Isolated from Avocado and Cucumber Fruits, and from an Avocado Processing Plant.

Listeria monocytogenes forms biofilms on food contact surfaces, a niche from where it dislodges to contaminate food products including fresh produce. Probiotics and their derivatives are considered promising alternative strategies to curb the presence of L. monocytogenes in varied food applications. Nonetheless, studies on their anti-biofilm effects against L. monocytogenes from avocados and cucumbers are sparse. This study screened the biofilm formation capabilities of L. monocytogenes strains Avo and Cuc isolated from the avocado and cucumber fruits respectively, and strain 243 isolated from an avocado processing plant; and evaluated the anti-biofilm effects of cell free supernatants (CFS) of Lactobacillus acidophilus La14 150B, Lactiplantibacillus plantarum B411 and Lacticaseibacillus rhamnosus ATCC 53103 against their biofilms formed on polyvinyl chloride (PVC) and stainless steel. All the L. monocytogenes strains formed biofilms (classified either as moderate or strong biofilm formers) on these materials. The presence of CFS reduced the biofilm formation capabilities of these strains and disrupted the integrity of their pre-formed biofilms. Quantitative reverse transcriptase polymerase chain reaction revealed significant reduction of positive regulatory factor A (prfA) gene expression by L. monocytogenes biofilm cells in the presence of CFS (p < 0.05). Thus, these CFS have potential as food grade sanitizers for control of L. monocytogenes biofilms in the avocado and cucumber processing facilities.

Attribution of country level foodborne disease to food group and food types in three African countries: Conclusions from a structured expert judgment study.

BackgroundAccording to the World Health Organization, 600 million cases of foodborne disease occurred in 2010. To inform risk management strategies aimed at reducing this burden, attribution to specific foods is necessary.ObjectiveWe present attribution estimates for foodborne pathogens (Campylobacter spp., enterotoxigenic Escherichia coli (ETEC), Shiga-toxin producing E. coli, nontyphoidal Salmonella enterica, Cryptosporidium spp., Brucella spp., and Mycobacterium bovis) in three African countries (Burkina Faso, Ethiopia, Rwanda) to support risk assessment and cost-benefit analysis in three projects aimed at increasing safety of beef, dairy, poultry meat and vegetables in these countries.MethodsWe used the same methodology as the World Health Organization, i.e., Structured Expert Judgment according to Cooke’s Classical Model, using three different panels for the three countries. Experts were interviewed remotely and completed calibration questions during the interview without access to any resources. They then completed target questions after the interview, using resources as considered necessary. Expert data were validated using two objective measures, calibration score or statistical accuracy, and information score. Performance-based weights were derived from the two measures to aggregate experts’ distributions into a so-called decision maker. The analysis was made using Excalibur software, and resulting distributions were normalized using Monte Carlo simulation.ResultsIndividual experts’ uncertainty assessments resulted in modest statistical accuracy and high information scores, suggesting overconfident assessments. Nevertheless, the optimized item-weighted decision maker was statistically accurate and informative. While there is no evidence that animal pathogenic ETEC strains are infectious to humans, a sizeable proportion of ETEC illness was attributed to animal source foods as experts considered contamination of food products by infected food handlers can occur at any step in the food chain. For all pathogens, a major share of the burden was attributed to food groups of interest. Within food groups, the highest attribution was to products consumed raw, but processed products were also considered important sources of infection.ConclusionsCooke’s Classical Model with performance-based weighting provided robust uncertainty estimates of the attribution of foodborne disease in three African countries. Attribution estimates will be combined with country-level estimates of the burden of foodborne disease to inform decision making by national authorities.

Levels of selected trace metals in enset (Ensete ventricosum (Welw.), Cheesman) (Unprocessed and processed) and soil of siltie zone, Southern Ethiopia.

Enset (Ensete ventricosum) is an important co staple crop for greater than 20 million people in southern and southwestern Ethiopia. In this study, the concentration of selected trace metals (Pb, Zn, Hg, Cd, Cu, Ni, Co, Fe, Mn, Cr and Pb) in enset pant, its food product and corresponding soil sampled from Siltie zone, Southern Nations, Nationalities, and Peoples Region of Ethiopia were determined. The known weights of oven-dried enset plant and food samples were digested in microwave digestion system whereas acid-wet digestion method was used for soil samples. The digests were subsequently analyzed for heavy metals content by inductively coupled plasma optical emission spectrometry (ICP-OES). The results showed that the relative abundance of trace metals in the enset sample was in the order Fe> >Mn>Zn>Cu>Ni>Pb>Cr≈Cd>Hg>Co and Fe> >Zn>Mn>Ni>Cu>Pb>Cr>Cd>Hg>Co in food samples. The results obtained were within the safe limits set by the FAO/WHO except for Pb (0.48 ± 0.09–3.68 ± 0.57) and Cd (1.04 ± 0.19 mg/kg) in samples from west Azerinet. Also, the result showed the concentrations of metals in the food products were significantly greater than that of enset plant, which shows there may have the potential to be contaminated during food manufacturing steps. The sampled soils were found to be between weakly acidic to weakly basic (pH: 6.15–7.62) and the levels of the trace metals were determined in the acceptable range recommended by FAO/WHO for agricultural soil. Thus, it is important to develop new or improved procedure for processing enset in order to counter attack the potentially toxic trace metals contamination of enset food products that may occur during fermentation process.

From Farm to Fork: Streptococcus suis as a Model for the Development of Novel Phage-Based Biocontrol Agents.

Bacterial infections of livestock threaten the sustainability of agriculture and public health through production losses and contamination of food products. While prophylactic and therapeutic application of antibiotics has been successful in managing such infections, the evolution and spread of antibiotic-resistant strains along the food chain and in the environment necessitates the development of alternative or adjunct preventive and/or therapeutic strategies. Additionally, the growing consumer preference for “greener” antibiotic-free food products has reinforced the need for novel and safer approaches to controlling bacterial infections. The use of bacteriophages (phages), which can target and kill bacteria, are increasingly considered as a suitable measure to reduce bacterial infections and contamination in the food industry. This review primarily elaborates on the recent veterinary applications of phages and discusses their merits and limitations. Furthermore, using Streptococcus suis as a model, we describe the prevalence of prophages and the anti-viral defence arsenal in the genome of the pathogen as a means to define the genetic building blocks that are available for the (synthetic) development of phage-based treatments. The data and approach described herein may provide a framework for the development of therapeutics against an array of bacterial pathogens.

Portable chemiluminescence optical fiber aptamer-based biosensors for analysis of multiple mycotoxins

Multiple mycotoxin contamination in foodstuffs causes significant threat to human health especially considering their synergistic effects. Herein, we present a novel chemiluminescence optical fiber aptamer-based biosensors for ultrasensitive on-site assay of multiplex mycotoxins in food samples. The optical fiber aptasensor is developed based on the specific recognition of single-stranded binding protein (SSB) and mycotoxin aptamers for the selective analysis. Optical fibers are utilized not only as the carrier to immobilize SSB to prepare the sensing region, but also as the transducer of the chemiluminescent emission to detect the sensor response. Multiple-channel fiber sensors are coupled together toward the detector and a compact and portable design of the chemiluminescence optical fiber aptasensor system is proposed, allowing four mycotoxins (AFB1, FB1, OTA or ZEN) to be simultaneously determined from one run without any tedious operation procedure. The optical fiber aptasensor system can perform sensitive analysis with a wide detection range from pg/mL to 105 pg/mL and low limit-of-detection in the range of 0.015 pg/mL-0.423 pg/mL for different target mycotoxins. The method is successfully applied for simultaneous and sensitive detection of multiple mycotoxins in infant cereals, showing its practical application in on-site analysis of real food samples.

Evaluation of canine raw food products for the presence of extended-spectrum beta-lactamase- and carbapenemase-producing bacteria of the order Enterobacterales.

To assess the potential contamination of commercial raw dog food products with bacteria of the Enterobacterales order that produce extended spectrum beta-lactamase (ESBL) and carbapenemase enzymes, determine risk factors for contamination, and understand isolate genetic diversity. A total of 200 canine raw food products. Products were cultured on selective chromogenic agar following enrichment steps. Whole-genome sequencing was performed for isolates that were confirmed to produce an ESBL. Isolates were characterized by antimicrobial resistance genes, and multilocus sequences typing, and compared to other isolates in the NCBI database for clonality. Preservation method and protein sources were assessed as potential risk factors for contamination with ESBL and carbapenemase-producing bacteria of the Enterobacterales order. No carbapenemase-producing Enterobacterales (CPE) were identified, but ESBL-producing Enterobacterales bacteria were isolated from 20/200 products (10.0%; 95% CI, 7.3 to 16.5%), all of which were frozen. Pork-derived protein source products were 8.1 times (P = .001; 95% CI, 2.53 to 26.2) more likely to carry ESBL-producing Enterobacterales bacteria than other protein sources. WGS analysis confirmed the presence of ESBL genes in a total of 25 distinct isolates (19 Escherichia coli, 5 Klebsiella pneumoniae, and 1 Citrobacter braakii). Genes encoding CTX-M type ESBL enzymes were the most common (24/25 isolates, 96.0%) with blaCTX-M-27 being the most common allele (8/25, 32.0%). Frozen, raw food products may serve as a route of transmission of ESBL-producing Enterobacterales bacteria to companion animals. Veterinarians should advise owners about the risks of raw food diets, including potential exposure to antimicrobial-resistant bacteria.

Martensitic Stainless Steels for Food Contact Applications

For industrial mechanical food processing steps, like cutting, slicing, grating, passing or similar applications, tools of martensitic stainless steels are used preferably because of their high hardness and corrosion resistance. The latter is decisive to avoid contamination of foodstuff by elements transferred from the steel alloy into the processed food. In this contribution various martensitic stainless steels in different heat treatment conditions were investigated regarding their metal release behavior in food simulants. Therefore, migrations tests were carried out according to the guideline of the Council of Europe. During the examination, the specific migration of elements was determined and compared to defined release limits, which take into account the different toxicity for the human body. The results allow an assessment of the suitability of the tested grades and heat treatment conditions as contact material for different types of food.

Co-culture with Acinetobacter johnsonii enhances benzalkonium chloride resistance in Salmonella enterica via triggering lipid A modifications

Salmonella enterica is one of the leading causes of foodborne gastroenteritis worldwide. In the food production environment, many bacterial species co-exist on surfaces in biofilm structures, which can act as reservoirs of microbial contamination of food products. Polymicrobial biofilms have been shown to have greater tolerance to antimicrobials, such as disinfectants, however the mechanistic basis of this is poorly understood. In this study, S. enterica subsp. enterica serovar Liverpool was co-cultured in mixed-species biofilms with bacteria isolated from the food production environment and challenged with the cationic biocide disinfectant, benzalkonium chloride (BC). Co-culture with the common environmental bacterium Acinetobacter johnsonii resulted in >200-fold higher resistance of S. Liverpool to BC, compared to mono-culture biofilms. The transcriptional response of S. enterica to biofilm co-culture was determined using a dual RNA-seq strategy. Genes controlled by the PhoPQ and PmrAB two-component systems, involved in lipid A modification and associated with cationic antimicrobial peptide resistance (CAMP) of S. Liverpool, were significantly upregulated. Deletion of either the phoP or pmrA genes resulted in an increase in susceptibility to BC, suggesting that activation of their regulons during co-culture enhances BC resistance. S. Liverpool lipid A profiles changed significantly upon co-culturing, with greater incorporation of both phosphoethanolamine and palmitate, which was dependent upon activation of PhoPQ and PmrAB. We conclude that when grown in the presence of A. johnsonii, S. Liverpool increases its tolerance to cationic BC disinfection by remodelling its cell envelope including reducing the net negative charge of lipid A and increasing lipid A acyl density.

Ultrasensitive immunosensing of Penicillin G in food samples using reduced graphene oxide (rGO) decorated electrode surface

The impact of uncontrolled antibiotic use in animals has subsequently led to emergence of antibiotic-resistant bacteria among humans due to consumption of animal by-products. Hence, to investigate antibiotic contamination in animal origin food products, we have developed a reduced graphene oxide (rGO) based immunosensor using fabricated electrode conjugated with anti-Penicillin antibody (rGO/Pen-Ab) for sensitive detection of Penicillin G. To execute this, Penicillin was first conjugated with Bovine Serum Albumin (BSA) which was confirmed via chromatographic, spectroscopic and electrophoretic-based techniques against both the in-house developed Penicillin conjugate (Pen-BSA) as well as the commercial Penicillin conjugate (Com-Pen-BSA). Further, we fabricated electrode based on one step synthesized rGO and immobilized with antibodies generated against Pen-BSA (Pen-Ab), and Com-Pen-BSA (Com-Pen-Ab), separately for detection of Penicillin. Each synthesis and conjugation step was confirmed by different spectroscopic methods. For efficient working of the electrode, various parameters were optimized using Voltammetry. The limit of detection for Penicillin G against Pen-Ab and Com-Pen-Ab was determined as 0.724 pM and 0.668 pM respectively and both displayed negligible cross reactivity against other β-lactam antibiotics (Cefalexin and Ampicillin). Furthermore, antibiotics were also detected in spiked milk, egg and meat samples and the electrode was evaluated for repeatability and storage stability. In conclusion, in-house developed Pen-Ab showed better sensitivity as compared to Com-Pen-Ab. The fabricated rGO/Pen-Ab biosensor shows future potential for rapid detection of penicillin and other β-lactam antibiotics for safe consumption of animal by-products in humans.

A mixture of pyrethroids induces reduced fecundity and increased testicular genotoxicity in rats

To achieve crop protection and higher agricultural yield, pesticides are used; and among them pyrethroid based ones are the most preferred choice because of their specificity on the pests. Uncontrolled use of pesticides resulted in contamination of food products. Presence of residual amounts of pyrethroids was reported in agricultural products sold in Indian market, indicating that humans are constantly exposed to these chemicals through food on a routine basis. Studies that determine the toxic effects of pyrethroids at doses equivalent to human exposure are rare. We orally administered a mixture of pyrethroids (detected in the rice and vegetables of Indian market) to male rats for 15 months to mimic the long-term exposure in humans. We observed reduced fecundity, sperm count and 13β-hydroxysteroid dehydrogenase enzyme activity. The serum concentrations of hormones involved in male reproductive function were altered. Further, testicular genotoxicity as reflected by perturbations in the expression pattern of genes involved in the molecular processes of gametogenesis was evident. Such toxic effects may also be occurring in humans who consume agricultural products that contain residual amounts of pyrethroids on a regular basis throughout their lifetime.

Gases in Food Production and Monitoring: Recent Advances in Target Chemiresistive Gas Sensors

The rapid development of the human population has created demand for an increase in the production of food in various fields, such as vegetal, animal, aquaculture, and food processing. This causes an increment in the use of technology related to food production. An example of this technology is the use of gases in the many steps of food treatment, preservation, processing, and ripening. Additionally, gases are used across the value chain from production and packaging to storage and transportation in the food and beverage industry. Here, we focus on the long-standing and recent advances in gas-based food production. Although many studies have been conducted to identify chemicals and biological contaminants in foodstuffs, the use of gas sensors in food technology has a vital role. The development of sensors capable of detecting the presence of target gases such as ethylene (C2H4), ammonia (NH3), carbon dioxide (CO2), sulfur dioxide (SO2), and ethanol (C2H5OH) has received significant interest from researchers, as gases are not only used in food production but are also a vital indicator of the quality of food. Therefore, we also discuss the latest practical studies focused on these gases in terms of the sensor response, sensitivity, working temperatures, and limit of detection (LOD) to assess the relationship between the gases emitted from or used in foods and gas sensors. Greater interest has been given to heterostructured sensors working at low temperatures and flexible layers. Future perspectives on the use of sensing technology in food production and monitoring are eventually stated. We believe that this review article gathers valuable knowledge for researchers interested in food sciences and sensing development.

Assessment of the antioxidant, antimicrobial and antibiofilm activities of essential oils for potential application of active chitosan films in food preservation.

In the food industry, the development of microbial biofilms is a serious problem that leads to the contamination and deterioration of food products. To overcome that, our aim consists of searching for natural antimicrobial and non-toxic compounds (essential oils EOs), which might be used alone or adsorbed on natural biopolymer films (chitosan). In this work, the antioxidant activity of eight EOs was evaluated by DPPH radical-scavenging method while their antibacterial activity was determined by diffusion on agar and microdilution methods. Among all tested EOs, Eugenia caryophyllus, Cinnamomum zeylanicum Blume and Thymus satureioides Cosson showed high antioxidant activities at the concentration of 25.6mg/mL, with respective values of (86.26%, 81.75%, and 76%), and strong antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Enterococcus hirae, with (MIC) values ≤ 4 µL/mL. At the concentration of 1 µL/mL, these EOs tested alone, showed values of antibiofilm-forming activity ranging from 79.43 to 99.33% and from 44.18 to 94.17%, when they are adsorbed onto chitosan film. These promising results confirm that these three EOs have a good potential for an eventual application in the food industry, as antimicrobial and antioxidant agents, or as active biodegradable food packaging, if combined with chitosan.

THE PREVALENCE OF BACTERIA OF THE GENUS PROTEUS SPP. IN DIFFERENT TYPES OF RESEARCH FOOD PRODUCTS

This article discusses the issue of contamination of food products by microorganisms of the genus Proteus, which actively multiply on a protein nutrient medium, causing the rotting of meat, fish, and other protein-rich foods. It is noted that recently in catering facilities there are often cases of toxic infections caused by the presence of these particular bacteria. The article draws attention to the fact that acute enteroidea, occurring as gastroenteritis, gastritis, or colienteritis, among young children, especially those with reduced immunity, is more severe, accompanied by symptoms of toxicosis, sometimes leading to complications up to hemolytic thrombopenia, anemia, acute renal impairment, etc. The article presents statistical materials obtained in the course of forensic research carried out in the Food Products and Drinks Expertise Department, which allow us to summarize the importance of strengthening control over the conduct of general sanitary measures and preventive measures in public catering facilities, in particular children’s institutions and hospitals. Key words: Proteus spp., pathogenicity, food poisoning, food products expertise, meat products.

Recent advances in radio frequency, pulsed light, and cold plasma technologies for food safety

AbstractIt has been the heartbeat of researchers and food engineers to discover the appropriate and effective technology for microbial control to ensure the safety of food. This is because microorganisms such as bacteria, fungi, viruses, and protozoa can cause contamination in food products. Many studies have published reviews on the use of single novel technology such as radio frequency (RF), pulsed light (PL), and cold plasma (CP) to process food to ensure food safety. However, no review has provided a comprehensive, detailed overview of the RF, PL, and CP treatments for pasteurizing food products in one publication. This study aims to review and present the future directions of RF, PL, and CP applications in light of various aspects of these technologies. The review concluded that although no novel technique (thermal or nonthermal) could adequately meet all the requirements for food safety, traditional thermal processes can be extremely useful in reducing and eliminating microbe contamination. However, they cannot be applied to temperature‐sensitive foods. Besides inactivating microbial spores, traditional thermal processes can denature proteins as well as organoleptic properties, such as taste, nutritional value, and sensory characteristics, illustrating the importance of these novel technologies as an alternative to heat‐based techniques. As a result of this study, hurdle technology is recommended since it has an additive effect and achieves better germicidal results.

Gold-Ceria nanocomposite based highly sensitive and selective aptasensing platform for the detection of the Chlorpyrifos in Solanum tuberosum

Contamination of food products due to the pesticide is a common problem. Pesticides, being one of the most harmful chemicals to human beings necessitates early and rapid on-site diagnostics to avoid chronic diseases due to their exposure. Their early and quick detection of edible products is crucial from the food safety point of view. We have fabricated an electrochemical aptasensing platform for the detection of Chlorpyrifos (CPF) using gold-ceria nanocomposite (Au@CeO2 nc). The electrochemical aptasensing platform has shown excellent linear response against CPF with good linearity of R2 = 0.96 in the range of 10 pM to 500 nM. The sensitivity of the aptasensor was evaluated from the slope of the calibration plot given by 12.68 mA nM−1 cm−2 and the limit of detection was estimated to be 0.12 pM. The applicability in vegetable samples was estimated by utilizing spiked cucumber (Cucumis sativus) and potato (Solanum tuberosum) samples of which Solanum tuberosum showed very good detection. Then extract from potato was used directly with increasing volume and studied against the electrochemical aptasensing platform where very good linearity and sensitivity were obtained. These studies demonstrate that the electrochemical aptasensing platform can be utilized for quick, label-free, and highly sensitive detection of pesticide, Chlorpyrifos in real samples.

Volatiles of antagonistic soil yeasts inhibit growth and aflatoxin production of Aspergillus flavus

Minimizing Aspergillus flavus growth is an effective strategy to mitigate aflatoxin contamination in food and agricultural products. In the present investigation, we attempted to utilize soil-associated yeasts from the Western and Eastern Ghats of India against A. flavus to reduce aflatoxin contamination. Forty-five yeast isolates were screened against A. flavus using overlay and dual plate assays. Among them, 12 isolates effectively inhibited the growth of A. flavus. The 18S rDNA gene sequence analysis identified the twelve antagonistic isolates as belonging to Saccharomyces cerevisiae, Suhomyces xylopsoci, Pichia kudriavzevii, and Candida tropicalis. From the isolated yeasts, S. cerevisiae strains were selected for further evaluation based on the potential antagonistic activity. Volatiles of S. cerevisiae effectively suppressed the mycelial growth of A. flavus (P < 0.05) up to 92.1 % at 7 DAI. Scanning electron microscopic images of the fungus exposed to volatiles showed hyphal deformity and mycelial damage. Aflatoxin B1 (AFB1) production was drastically reduced up to 99.0 % in the volatile-exposed fungus compared to the control. The yeast strain YKK1 showed consistent Aspergillus flavus growth inhibition (80.7 %) and AFB1 production (98.1 %) for 14 days. Gas chromatography-mass spectrophotometry analysis of the yeast volatiles revealed the presence of antimicrobial compounds, including 1-pentanol, 1-propanol, ethyl hexanol, ethanol, 2-methyl-1-butanol, ethyl acetate, dimethyl trisulfide, p-xylene, styrene, and 1,4-pentadiene. The evaluated compounds of yeast volatiles, including ethyl acetate, hexanal, 1-propanol, 1-heptanol, 1-butanol, and benzothiazole, inhibited the fungal growth and AFB1 production of Aspergillus flavus when applied as pure chemicals. Benzothiazole at 5 mM was responsible for a high level of growth inhibition (23.6 %) and reduction of AFB1 synthesis (93.5 %). Hence, volatile compounds produced by soil yeast strains could be a potential biocontrol mechanism against aflatoxin contamination.

Per- and polyfluoroalkyl substances (PFASs) in consumable species and food products.

Per- and polyfluoroalkyl substances (PFASs) are a group of thousands of manmade chemicals widely used in consumer products and industrial processes. Toxicological studies have suggested that exposure to PFASs may lead to several adverse effects, including infertility and cancer development. In light of their widespread use, the contamination of food products has created health concerns in sites directly influenced by industrial and anthropogenic activity. In the present contribution, the current knowledge of PFAS contamination was systematically reviewed in order to provide with the knowledge gaps and main sources of contamination, as well as critically evaluate estimated dietary intake and relative risk values of the consulted studies. Legacy PFASs remain the most abundant despite their production restrictions. Edible species from freshwater bodies exhibit higher PFAS concentrations than marine species, probably due to low hydrodynamics and dilution in lentic ecosystems. Studies in food products from multiple sources, including aquatic, livestock, and agricultural, agree that the proximity to factories and fluorochemical industries rendered significantly higher and potentially hazardous PFAS contamination. Short-chain PFAS are suggested as chemicals of emerging concern to food security. However, the environmental and toxicological implications of short-chain congeners are not fully understood and, thus, much research is needed in this sense.

Loop-mediated isothermal amplification (LAMP) method for fast detection of Campylobacter spp in meat food products and environmental objects of a processing plant’s

There is constant necessity of developing the accurate and fast methods for detection of foodborne pathogens. Microorganisms of Campylobacter genus are one of the main causes of foodborne diseases worldwide. Fast identification of Campylobacter at all stages of the food life cycle is an efficient strategy to control foodborne campylobacteriosis. This article the authors evaluated a commercial loop-mediated isothermal amplification (LAMP) system with bioluminescence, called as the 3M™ Molecular Detection Analysis (MDA), which was used to find Campylobacter in food products with the help of a certain standard method, which is referred to as the reference method. The results of this study showed that the commercial LAMP based method is as efficient as the reference method, and features high specificity and minimum determinability (sensitivity). The LAMP based method has been shown to be a fast and reliable method for detection of Campylobacter spp. scarce presence (10 CFU/25 g) in meat, meat products, as well as carcass swabs and production facilities’ environment. The LAMP analysis required about 24–27 hours to achieve a result. However the LAMP based method will facilitate the detection of Campylobacter, as it provides much easier and faster detection of Cam pylobacter spp., including Campylobacter jejuni/Campylobacter coli, than standard microbiological methods. The LAMP based method is an efficient tool to prevent the spreading of Campylobacter spp. contamination in food products.

Hordeum vulgare L. microgreen mitigates reproductive dysfunction and oxidative stress in streptozotocin-induced diabetes and aflatoxicosis in male rats.

Diabetes mellitus type 2 (DM) is a common chronic disease worldwide, which may be due to increased environmental pollution. Aflatoxin B1 is a likely inevitable contaminant in food and dairy products. Both DM and aflatoxicosis exert a deleterious effect on reproduction urging the exploration of various functional food for protection. This study investigated the effect of barley microgreen (BM) on reproductive disorders caused by DM with or without aflatoxicosis in male rats. Rats were divided into eight groups; G1 control, G2 barley, G3 aflatoxin, G4 aflatoxin‐barley, G5 streptozotocin (STZ), G6 STZ‐barley, G7 STZ‐aflatoxin, and G8 STZ‐aflatoxin‐barley. BM chemical composition revealed elevated calcium, iron, phosphorus, and vitamin A compared with barely seeds. Complete blood picture, lipid profile, serum oxidative stress parameters, relative testicular weight, sperm analysis, chromosomal aberration, and testis histopathology were performed. The lipid profile was altered significantly in G7. Oxidative stress was increased in G3, G5, and G7, whereas it was decreased in BM‐treated groups. Sperm counts were reduced significantly in aflatoxin and/or STZ groups but increased significantly in BM‐treated groups. Sperm morphological abnormalities and chromosomal aberrations were decreased significantly in BM‐treated groups compared with untreated groups. Testicular histopathology revealed moderate diffuse degeneration of seminiferous tubules in aflatoxin and/or STZ groups, which were alleviated in BM‐treated groups. In conclusion, aflatoxin and STZ together caused severe reproductive disorder and oxidative stress more than aflatoxin or STZ alone. BM diet reduced significantly oxidative stress and reproductive disorder associated with DM and aflatoxicosis in rats.

Cold plasma for microbial safety: Principle, mechanism, and factors responsible

AbstractElimination of microbiological contamination of food products is predominant for consumer food safety. Cold plasma (CP) technology has evolved as a unique, cost‐effective, and environmentally friendly solution to microbial decontamination that is gaining popularity due to its great efficacy in ensuring food safety. As increasing the demand of food industries to produce and supply the safe food with minimal processing. Cold plasma application is being used to sanitize the food surface to be used for different food industry sectors for microbial inactivation. The rapid microbial inactivation obtained by cold plasma has sparked a surge in interest in the field. The current review will give a critical snapshot of the potential application of cold plasma in microbial safety, including food safety, quality, and enzyme inactivation. The rapid microbiological inactivation achieved with cold plasma has led to an interest in this subject. Further, factors affecting, that is, food composition, PH, water activity, and microorganism type for microbial inactivation by cold plasma, are summarized and discussed. There is an increasing demand for food industries to produce and supply safe food with minimal processing. Cold plasma has the potential to sanitize the food surface to be used in different food industry sectors for microbial inactivation.Novelty impact statementThis review discussed the principle, equipment, and process mechanism of cold plasma. The mechanism of microbial inactivation by cold plasma was summarized. Also, the effect of cold plasma on factors including food composition, pH, water activity, microorganism type, and enzyme inactivation was explored.