A common method for characterizing microplastics (MPs) involves capturing the plastic particles on a filter after extraction and isolation from the sediment particles. Microplastics captured on the filter are then scanned with Raman spectroscopy for polymer identification and quantification. However, scanning the whole filter manually using Raman analysis is a labor-intensive and time-consuming process. This study investigates a subsampling method for Raman spectroscopic analysis of microplastics (operationally defined here as 45–1000 μm in size) present in sediments and isolated onto laboratory filters. The method was evaluated using spiked MPs in deionized water and two environmentally contaminated sediments. Based on statistical analyses, we found quantification of a sub-fraction of 12.5 % of the filter in a wedge form was optimal, efficient, and accurate for estimating the entire filter count. The extrapolation method was then used to assess microplastic contamination in sediments from different marine regions of the United States.