Containers For Long-term Storage Research Articles

Development of a Model for Long-Term Storage Container for VVER-1000 Spent Fuel Assemblies in ANSYS CFX

This paper evaluates the thermohydraulic aspects of modeling multi-purpose container (MPC-31) with VVER-1000 spent nuclear fuel (SNF) during its long-term storage in HI-STORM container (designed by Holtec International). The paper describes the main approaches and assumptions applied for the development of correspondent ANSYS CFX model, as well as provides the main calculation results for one of the cases of MPC-31 loading with SNF. The results of calculations were used in the regulatory review of technical specifications of MPC-31 and correspondent safety analysis reports.

廃棄物長期保管容器内に発生する水素ガスの濃度低減のための基礎実験計画と予備解析

廃棄物長期保管容器内に発生する水素ガスの濃度低減のための基礎実験計画と予備解析

The Use of Cavitation Technology in the Treatment of Spent Nuclear Fuel Processes

Are set out positive results of the application impulse cavitation technology for handling waste management reprocessing of spent nuclear fuel is to enhance the safety, durability, of their storage and dumping for improve strength of the cement compound. Is proposed for numerical model for calculating of the thermal container for long-term storage of radioactive waste allowing improve accuracy of calculations of data of the design for objects of waste storage. It was established experimentally that the use of cavitation allows to increase outlet poorly soluble components pulp compared to conventional recycling when extracting them out storage tanks.

Cryopreservation of Gemmae from the Liverwort Marchantia polymorpha L.

The liverwort Marchantia polymorpha L. is one of the key model plants in evo–devo studies, and an increasing number of transgenic and mutant lines have been established. For reliable long-term preservation of M. polymorpha plants, spores have been used, but crossing is indispensable to obtain them. Gemmae, however, are vegetative clones and readily available in large numbers without crossing, thereby enabling the clonal preservation and rapid propagation of transgenic or mutant lines. Here, we report a simple cryopreservation protocol for in vitro grown M. polymorpha gemmae using aluminum cryoplates. Gemmae were pre-cultured on sucrose-containing medium, embedded in calcium alginate gel on the surface of a cryoplate, moderately dehydrated and stored in liquid nitrogen. After rapid thawing, the stored gemmae showed a 100% survival rate. Our protocol does not require plant growth regulators such as ABA, and takes only 1 h to complete except for 1 d of pre-culture. Furthermore, gemmae treated as described above but then air-dried for 2 h can be stored at −80°C for at least 1 year without a significant decrease in survival rate, which is convenient for most laboratories that have a −80°C freezer but not a liquid nitrogen container for long-term storage. These preservation techniques for M. polymorpha should increase their availability in the research community.

Design of Enzyme-Encapsulated Protein Containers by In Vivo Crystal Engineering.

Crystalline protein assemblies of polyhedra crystal (PhC) can be utilized as solid enzyme containers for long-term storage of enzymes with retention of their enzymatic activity. The enzymes can be released from the crystals at the optimum pH for the enzymatic activity by dissolution of the crystals using in vivo crystal engineering.

Soft Plastic Bag Instead of Hard Plastic Container for Long-term Storage of Breast Milk

To compare the fat content and contamination of expressed breast milk (EBM) before and after storage (30 d) in hard polypropylene containers (HC) and soft polyethylene bags (SB) containers. Ninety specimens of EBM were collected into HC and separated into two HC and two SB. The fat content of each specimen of EBM in HC and SB was measured and cultures were performed. The specimens in the second HC and SB containers were kept frozen for 30 d before thawing and then measuring the fat content and performing cultures. The means ± SD of the fat content of fresh and thawed EBM in HC were 2.98 ± 0.97 and 2.66 ± 0.88 g/100 ml, respectively, with a loss of 0.32 g/100 ml (p < 0.001). The means ± SD of the fat content of fresh and thawed EBM in SB were 3.06 ± 1.00 and 2.77 ± 0.91 g/100 ml, respectively, with a mean loss of 0.29 g/100 ml during storage (p < 0.001). The loss of fat content during frozen storage did not differ significantly between the two types of containers (p = 0.53). All bacterial cultures of fresh and thawed EBM in HC and SB showed only nonpathogenic organisms. SB can replace HC for the long-term storage of frozen EBM of up to 30 d without deleterious effects on fat loss or contamination.

Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics

The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method.

Assessment of Corrosion-Based Failure in Stainless Steel Containers Used for the Long-Term Storage of Plutonium-Based Salts

Abstract This paper summarizes our efforts to assess corrosion-related failure in stainless steel long-term storage containers bearing plutonium oxides and electrorefining salts. Pitting corrosion of the internal can wall is believed to occur when these salt particles deliquesce forming the electrolyte necessary for corrosion-electrochemistry. Extrapolation of pit depths from coupon studies using generalized extreme value (GEV) statistics found that the probability of a through-wall corrosion pit is finite; the maximum pit depth after 50 years would be on the order of 1.7 mm where the container wall is only 1.6 mm thick. To assess susceptibility to environmental cracking fracture toughness (J1C), experiments were used in conjunction with a J-integral diagram constructed using the GE/EPRI method for linear elastic-plastic materials. As apart of this analysis, the residual stress associated with the weld was measured using the laser contour method. The hoop stress in the weld region was found to be on the o...