Bovine Pleuropneumonia Research Articles

Mathematical Modelling of the Transmission Dynamics of Contagious Bovine Pleuropneumonia with Vaccination and Antibiotic Treatment

In this paper we present a mathematical model for the transmission dynamics of Contagious Bovine Pleuropneumonia (CBPP) by considering antibiotic treatment and vaccination. The model is comprised of susceptible, vaccinated, exposed, infectious, persistently infected, and recovered compartments. We analyse the model by deriving a formula for the control reproduction number Rc and prove that, for Rc<1, the disease free equilibrium is globally asymptotically stable; thus CBPP dies out, whereas for Rc>1, the unique endemic equilibrium is globally asymptotically stable and hence the disease persists. Thus, Rc=1 acts as a sharp threshold between the disease dying out or causing an epidemic. As a result, the threshold of antibiotic treatment is αt⁎=0.1049. Thus, without using vaccination, more than 85.45% of the infectious cattle should receive antibiotic treatment or the period of infection should be reduced to less than 8.15 days to control the disease. Similarly, the threshold of vaccination is ρ⁎=0.0084. Therefore, we have to vaccinate at least 80% of susceptible cattle in less than 49.5 days, to control the disease. Using both vaccination and antibiotic treatment, the threshold value of vaccination depends on the rate of antibiotic treatment, αt, and is denoted by ραt. Hence, if 50% of infectious cattle receive antibiotic treatment, then at least 50% of susceptible cattle should get vaccination in less than 73.8 days in order to control the disease.

A participatory epidemiological study of major cattle diseases amongst Maasai pastoralists living in wildlife-livestock interfaces in Maasai Mara, Kenya

Livestock-wildlife interactions promote the transmission of a wide range of infectious diseases that constraint livestock production. We used a participatory appraisal approach to find out and rank infectious diseases of concern to pastoralists in a zone of intense wildlife-livestock interaction and another zone with limited interactions. Four villages were selected purposively in areas with intensive cattle-wildlife interactions (zone 1), and another two in areas with low to moderate cattle-wildlife interactions (zone 2). Data were collected in focus group discussions (FGDs) using participatory epidemiological methods (PE); each group had 8–13 participants. Results of impact matrix scoring from all sites indicated that malignant catarrhal fever (MCF), anthrax, foot and mouth disease (FMD), contagious bovine pleuropneumonia (CBPP), east coast fever (ECF) and African animal trypanosomiasis (ATT), in decreasing order, had the highest impact on livestock production. A Kruskal–Wallis test revealed a significant difference in FMD annual prevalence between cattle age groups (p < 0.001) and was the highest in animals > 4 years (median score of 32.5, range, 10–50). FMD had the highest impact on milk production, but based on veterinary costs (treatment costs), it was ranked second to CBPP. The study provides information on disease priorities that occur in the target zones in Mara ecosystem and which the local pastoralists must consider when accessing key ecosystem services such as water and pasture.

Molecular Detection and Pathological Investigation of Contagious Bovine Pleuropneumonia in Selected Districts of Punjab, Pakistan

Molecular Detection and Pathological Investigation of Contagious Bovine Pleuropneumonia in Selected Districts of Punjab, Pakistan

The experience of contagious bovine pleuropneumonia ring trials in Botswana.

The Botswana National Veterinary Laboratory (BNVL) has conducted ring trials (proficiency testing) on an annual basis since 2010. Proficiency testing is carried out to evaluate the ability of veterinary laboratories to perform serological complement fixation tests (CFTs) and molecular polymerase chain reaction (PCR) tests for the diagnosis of contagious bovine pleuropneumonia (CBPP). In this paper, the authors discuss the experience gained and the lessons learned in coordinating these ring trials over a period of six years, from 2010 to 2015.The number of participating laboratories increased from five in 2010 to 11 in2015. Their performance also improved over this period. The proportion of unsatisfactory results decreased from 40% to 10% for serological testing, while questionable results decreased from 60% to 10%. The proportion of unsatisfactory results for the molecular test decreased from 33% to 0%. Systematic errors (i.e. technical errors or imperfect experimental design) were the principal causes of questionable and unsatisfactory results. An analysis of responses from customer satisfaction surveys conducted annually since 2013 provided valuable information that enabled BNVL to redesign the programme in 2014 and 2015 to improve the overall quality of the proficiency testing programme. Among the changes made were sending freeze-dried sera for CFTs and DNA for PCR instead of sera and liquid cultures.

Vaccination coverage of contagious bovine pleuropneumonia in Adamawa State, Northeastern Nigeria

Contagious bovine pleuropneumonia is an important, transboundary animal disease of high socio-economic impacts and vaccination has been recommended as one of the preferred option for its control. Twelve years (2006-2017) of data were retrieved from the Ministry of Livestock Production, Adamawa state. The relevant data collated included; estimated annual cattle population, number of cattle vaccinated against CBPP, number of CBPP outbreaks reported, number of mortality due to the disease, number of cattle slaughtered and examined for CBPP lesions and number of cattle with suspected CBPP lesions. The overall vaccination coverage of 4.80% was obtained with the highest vaccination coverage (20.57%) and the lowest vaccination coverage (1.03%) recorded in 2013 and 2015 respectively. A negative and insignificant correlation (r= -0.2346, p&gt; 0.05) was obtained between vaccination coverage and prevalence, whereas correlation between prevalence and reported outbreak of CBPP was positive (r= 0.7381). A total of 76 outbreaks representing an average of 6.33 outbreaks per year and a mortality of 456 were recorded. The highest number of outbreaks (15) was recorded in 2017 whereas the least number of outbreaks (1) was recorded in 2007. The study has established low and irregular vaccination coverage which resulted in increased outbreaks of the disease in the study area. The need for Government to procure CBPP vaccines that is affordable as well as accessible to cattle owners at subsidized rates, increased and compulsory vaccination coverage of 80% for 5 consecutive years and enlightenment of cattle owners through public media on the dangers of the disease was recommended.Keywords: Contagious bovine pleuropneumonia, outbreak, vaccination coverage, Adamawa state

Serological Prevalence of Contagious Bovine Pleuropneumonia in Niger in 2017.

Contagious bovine pleuropneumonia (CBPP) is a highly contagious disease of cattle caused by Mycoplasma mycoides subsp. mycoides Biotype Small Colony (MmmSC). The disease currently occurs in most of sub-Saharan Africa and where it is endemic and a major constraint for improving pastoral productivity. Following the persistence of this scourge, and in order to control this disease, a serological survey was conducted to determine the prevalence of CBPP in Niger. In fact, 1,590 sera were collected following a stratified sampling plan based on the risk factor of dissemination of CBPP. The analysis were performed at the Central Livestock Laboratory using the c-Elisa test. The results obtained show a wide distribution of the disease with an overall prevalence of 4.15% at individual level. The highest prevalences were recorded in the South-East regions [Zinder (7.5%), Diffa (7.5%)] and the West part [Tahoua (6.9%)]. The prevalence at the commune level was about 36.55%, which was relatively high. The prevalence at strata level was 36.55% (95% PI 0.2428–0.4882). The expected prevalences did not match those found. The results of this serological survey are considered the reference situation (T0) of CBPP in Niger with the PRAPS project, and allowed to the country to redefine control policies for better control of the disease at national and sub-regional level.

Contagious bovine pleuropneumonia within the 19th century miasmatic landscape.

When contagious bovine pleuropneumonia (CBPP) was first detected on a farm north of Melbourne, at Bundoora, in 1858, the predominant theory of miasma was being challenged by contagionist theories of disease transmission. This well-documented case was recorded during a period of change in the scientific assessment of disease and therefore affords an exploration of what aspects of the landscape were considered important for livestock health at the time. Although the introduction, vaccination programs and eventual eradication of CBPP on mainland Australia has been well explored, scholars have neglected this aspect of the disease's history. By comparing 19th century records of farmland with how the site appears today, it is also possible to highlight the limited information provided by contemporary texts, while at the same time developing an appreciation of the ways in which the perception of the rural landscape has changed. This differing perception has implications for the utilisation of these sources for veterinary and environmental historians seeking to understand the mid-19th century agricultural landscape and how it relates to animal health.

Climate change perception and impacts on cattle production in pastoral communities of northern Tanzania

This study examines pastoralists’ perception on climate change and impacts on cattle production from ten study villages of Monduli District in Tanzania. This study drew empirical data from 130 cattle owners and 81 participants from focus group discussions in the study villages. Descriptive statistics and thematic analysis were used to analyze the data respectively. Findings from this study indicate that pastoralists are well aware of the general climate trends in their location, its variability and the impacts of extreme weather events on cattle production. The main climate changes perceived by pastoralists include more erratic and reduced amounts of rainfall, rise in temperature and prolonged and frequent periods of drought. Pastoralists mainly view population pressure and tree cutting as the major causes of climate change. Further, this study found that there are no existing climate risk programmes and early warning systems across the district. Importantly, pastoralists reported the negative impacts of climate change on cattle production. The severe recurrent drought periods result in shortage of forage and water, leading to cattle starvation and malnutrition. Pastoralists reported massive cattle deaths and outbreaks of diseases such as contagious bovine pleuropneumonia and tick-borne diseases. Further, reduction in milk production and poor livestock market prices were also reported as negative impacts on cattle production. Given the range of negative impacts of current climate change and extreme weather events on cattle production, the implications of climate change must be taken into account to ensure longer-term survival and sustainability of pastoralist communities through strengthening of climate risk awareness programs and early warning systems across the district. Further, this study recommends that government and non-governmental organizations should improve institutional support to pastoralists in order to help them deal with the negative impacts of climate change on livestock production.

Review of Participatory Epizootiology Research of Contagious Bovine Pleuropneumonia at the Department of Veterinary Public Health and Preventive Medicine, University of Ibadan, Nigeria (2007-2015)

This is a review of research application of Participatory Epizootiology to investigate Contagious Bovine Pleuropneumonia among pastoralists in Oyo State and Niger State, Nigeria, between 2007 and 2015, at the Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ibadan where the first author, a Veterinary Epidemiologist Lecturer/Researcher teaches Participatory Epizootiology Research as part of PVM 711: Advanced Epizootiology, a compulsory Course in the three Master degrees curriculum of the Department since 2004. He subsequently designed a PhD research project on the application of Participatory Epizootiology to the Igangan Grazing Reserve and got a University Senate Research SRG/FVM/2006/9A to that effect. In 2007, the Principal Veterinary Officer of the Faculty Eruwa Veterinary Field Station in Ibarapa East Local Government Area, Oyo State, south-western Nigeria, reported to the first author (then Chairman, Eruwa Veterinary Field Station), a suspected case of Contagious Bovine Pleuropneumonia incidence at the Igangan Grazing Reserve. The diseased lung samples of dead infected cattle he brought were confirmed by gross and histopathological examinations at the Faculty&rsquo;s Department of Veterinary Pathology. From 2011 to 2015, the second author adopted the PE PhD-design (with other conventional veterinary research approach) to investigate Contagious Bovine Pleuro-pneumonia prevalence in Niger State, North-central Nigeria, first for his Masters (2011), followed with a PhD (2015). Result indicated that Contagious Bovine Pleuropneumonia in enzootic in Oyo and Niger State, Nigeria with a 77.67% of CBPP outbreaks in Niger State, Nigeria occurring in the dry season, and a prevalence of 8.73%. Participatory Epizootiology is a cheap, low capital involvement of traditional settler beneficiaries in identification of enzootic animal problems and their Community Based Animal Health training need. Participatory Epizootiology should be adopted in combination with conventional veterinary methods for effective Contagious Bovine Pleuropneumonia surveillance and control techniques in Africa

Contagious Bovine Pleuropneumonia Vaccine Delivery and Adoption by Women and Men in North-Eastern Kenya

Contagious bovine pleuropneumonia (CBPP) is an endemic transboundary disease of cattle controlled by vaccination, with a vaccine characterized by low efficacy and safety. To contribute towards its eradication, social scientists have sought ways of improving the delivery and adoption of the vaccine, whereas vaccine scientists have attempted to develop safer and more efficacious vaccines. To understand the status of CBPP vaccine delivery and the effect of gender on vaccine adoption, qualitative and quantitative data were collected from vaccine delivery stakeholders, including men and women cattle owners from north-eastern Kenya. The results indicate that the main constraints to vaccine delivery include restricted distribution due to stringent government regulation and the need for a cold supply chain, which is exacerbated by poor transport infrastructure. On vaccine adoption, men and women accepted the vaccine, but men were willing to pay significantly higher prices than women because they were significantly wealthier.

Increasing the Local Relevance of Epidemiological Research: Situated Knowledge of Cattle Disease Among Basongora Pastoralists in Uganda.

Cattle disease can have severe negative impacts on the livelihoods of the poor, but still, animal disease management and outreach often remain suboptimal in low-income settings. In a study on Basongora pastoralists in Uganda, we examined local priorities, perceptions and practices regarding cattle disease, in order to improve outreach and disease control advisory work in such contexts. We also investigated how participatory epidemiology can be better equipped for gathering situated knowledge. Empirical material obtained in focus group discussions, interviews, participatory mapping, and wealth-ranking was used to perform a thematic, bottom-up analysis. The concepts of situated knowledge and embodied objectivity and insights from participatory research and interdisciplinary dialogue were applied to better embrace local perspectives. Cowdriosis, trypanosomosis, contagious bovine pleuropneumonia, East Coast fever and anthrax were high-priority diseases for participants. Lack of control over the animal health situation and money invested in treatments that did not guarantee recovery were of general importance for disease prioritization. Participants' descriptions of diseases sometimes diverged from textbook definitions. Co-infections, chronic and recurring infections and lack of access to formal knowledge were identified as important factors for differences between formal and situated knowledge. Paying attention to situated knowledge and particular context-specific issues such as proximity to a national park proved to be of special relevance for local understanding and experiences with disease. Another factor was the local importance ascribed to number of cattle, rather than production levels. These factors need to be taken into consideration when formulating disease control advice, as does the complex disease landscape. The results reveal the importance of moving research and advice beyond curing “knowledge-gaps” and creating different ways of understanding disease so that situated knowledge can be considered, and disease control improved.

Polymorphonuclear cells and reactive oxygen species in contagious bovine pleuropneumonia: New insight from in vitro investigations

Reactive oxygen species (ROS) are suggested to play a role in the pathogenesis of contagious bovine pleuropneumonia, a severe respiratory disorder caused by Mycoplasma mycoides subsp. mycoides (Mmm). The present study investigated the generation of ROS by different strains of Mmm, as well as their effect on the oxidative response of bovine neutrophils. The production of ROS was indirectly measured using a luminol-based chemiluminescence assay. Our results confirm that Mmm can produce ROS via the metabolism of glycerol, significant differences existing between African and European strains. Mmm was capable of adhering to the external surface of neutrophils. Interestingly, Mmm enhanced the respiratory burst of bovine neutrophils. This activity was particularly pronounced with the African field strain and in presence of glycerol. Taken together, our data argue in favour of a major role for neutrophils as the main source of ROS in contagious bovine pleuropneumonia.

Methodological aspects of serosurveillance in resource-poor settings

Animal production is important for the agricultural economy in low-income countries, but is threatened by infectious diseases. Serosurveys are conducted for different reasons such as disease detection, risk factor studies,...

Prevalence of contagious bovine pleuropneumonia based on gross lesions in cattle at slaughter in Adamawa State, Nigeria

Contagious bovine pleuropneumonia is an important economic disease of cattle caused by Mycoplasma mycoides subspecies mycoides and manifested by anorexia, fever, dyspnoea, polypnoea, cough and nasal discharges. An eleven years (2006-2016) abattoir-based retrospective data were collated and analysed from Adamawa state Ministry of Livestock Production. Out of 241,700 cattle examined at post-mortem, 8,429 had CBPP-like lesions giving a prevalence of 3.49% (95% CI: 3.41-3.56). The overall prevalence for each local government area were 2.42% (95% CI: 2.35-2.49), 5.53% (95% CI: 5.30-5.74) and 8.97% (95% CI: 8.56-9.41) for Yola, Mubi and Ganye abattoirs respectively. There was significant association (p<0.05) between Local Government Areas and occurrence of CBPP. The annual highest prevalence of 5.75% (95% CI: 5.43-6.09) was recorded in 2010 with 1,128 cases and lowest prevalence of 2.43% (95% CI: 2.23-2.66) was recorded in 2007 with 505 cases. Based on season, highest prevalence rate of 3.85% (95% CI: 3.58-4.14) was recorded in February with 709 cases and lowest prevalence rate of 3.03% (95% CI: 2.80-3.28) was recorded in December with 605 cases. Both annual and monthly prevalence rates varied significantly (p<0.05). It was concluded that CBPP is endemic and widespread in Adamawa state. Hence, we recommend increased funding in the control of the disease, restriction of cattle movement and provision of cattle ranches. Keywords: Adamawa state, Cattle, Contagious bovine pleuropneumonia, Gross lesions, Prevalence

Respiratory explants as a model to investigate early events of contagious bovine pleuropneumonia infection

Contagious bovine pleuropneumonia (CBPP) is a severe disease caused by Mycoplasma mycoides subsp. mycoides (Mmm). Knowledge on CBPP pathogenesis is fragmented and hampered by the limited availability of laboratory animal and in vitro models of investigation. The purpose of the present study is to assess respiratory explants as useful tools to study the early stages of CBPP. Explants were obtained from trachea, bronchi and lungs of slaughtered cattle, tested negative for Mycoplasma spp. and for the major bacterial and viral respiratory pathogens. The interaction of Mmm with explant cells was studied by immunohistochemistry (IHC), double-labelling indirect immunofluorescence (DLIIF) and laser scanning confocal microscopy (LSCM). Mmm capability to survive and proliferate within the explants was evaluated by standard microbiological procedures. Finally, the putative cellular internalization of Mmm was further investigated by the gentamicin invasion assay. IHC and DLIIF indicated that Mmm can colonize explants, showing a marked tropism for lower airways. Specifically, Mmm was detected on/inside the bronchiolar and alveolar epithelial cells, the alveolar macrophages and the endothelial cells. The interaction between Mmm and explant cells was abolished by the pre-incubation of the pathogen with bovine anti-Mmm immune sera. Mmm was able to survive and proliferate in all tracheal, bronchial and lung explants, during the entire time course of the experiments. LSCM and gentamicin invasion assay both confirmed that Mmm can enter non-phagocytic host cells. Taken together, our data supports bovine respiratory explants as a promising tool to investigate CBPP, alternative to cattle experimental infection.

Contagious Bovine Pleuropneumonia: Seroprevalence and Risk Factors in Gimbo District, Southwest Ethiopia.

Contagious bovine pleuropneumonia (CBPP) is a highly contagious disease of cattle which is one of the great plagues which continues to devastate the cattle herds on which so many people are dependent in Africa. Cross-sectional study was conducted from October 2015 to August 2016 to determine the seroprevalence of CBPP in cattle and associated risk factors in Gimbo district, Southwest Ethiopia. A total of 384 serum samples were collected and tested for the presence of specific antibodies against Mycoplasma mycoides subspecies mycoides small colony (MmmSC), using a competitive enzyme-linked immunosorbent assay (cELISA). Univariable and multivariable logistic regression analysis were performed to determine the association between risk factors and seroprevalence of CBPP. An overall seroprevalence of CBPP was 8.1% (31/384) and it was ranging from 0% to 20% across different Peasant associations (PAs). The seroprevalence of CBPP among adult animals was 8.5% (25) and in young 6.6% (6), in good body condition animals 6.6% (18) and in poor 11.5% (13), in dry season 11.9% (20) and in rainy 5.1% (11), and in highland altitude 2.5% (3), midland 3.8% (5), and lowland 17.4% (23). Among the potential predisposing factors assessed, altitude was found significantly (p = 0.02, OR = 7.3) associated with the seroprevalence of contagious bovine pleuropneumonia and other risk factors had no significant (P > 0.05) influence. The present study showed that the overall seroprevalence of CBPP in Gimbo district was high and this indicates a need for intervening and implementing control measures to prevent further spread of the disease in the district through the use of better and coordinated vaccination program.

Preliminary Findings of Lipoprotein B in Detecting Cattle Chronically Infected with Contagious Bovine Pleuropneumonia

Contagious Bovine Pleuropneumonia (CBPP) caused by Mycoplasma mycoides subsp. mycoides is an important disease of cattle affecting international trade. Complement Fixation Test (CFT) and competitive Enzyme Linked Immunosorbent Assay (c-ELISA) are the only serological tests recognized for diagnosis of CBPP. The performance of CFT depends on the quality of the antigen and the combination of reagents used and only detects CBPP during the acute phase of the disease. There is a need to develop a more sensitive and specific test. Therefore, one mycoplasma protein previously identified as being a potential diagnostic antigen, Lipoprotein B (LppB), was tested for its usefulness to detect infected cattle in an indirect ELISA (i-ELISA). Samples obtained from pre-challenged and challenged cattle were compared using CFT and an i-ELISA based on lipoprotein B. The i-ELISA developed with the new antigen, detected more positive samples than the CFT, which is considered a gold standard. Therefore, the LppB recombinant protein could be explored further as target for screening cattle infected chronically with CBPP.

Analysis of a mathematical model of the dynamics of contagious bovine pleuropneumonia

Contagious bovine pleuropneumonia (CBPP) is a disease of cattle and water buffalo caused by Mycoplasma mycoides subspecies mycoides (Mmm). It attacks the lungs and the membranes that line the thoracic cavity. The disease is transmitted by inhaling droplets disseminated through coughing by infected cattle. In this paper a deterministic mathematical model for the transmission of Contagious Bovine plueropnemonia is presented. The model is a five compartmental model consisting of susceptible, Exposed, Infectious, Persistently infected and Recovered compartments. We derived a formula for the basic reproduction number R0. For R0 ≤ 1, the disease free equilibrium is globally asymptotically stable, thus CBPP dies out; whereas for R0 &gt; 1, the unique endemic equilibrium is globally asymptotically stable and hence the disease persists. Elasticity indices for R0 with respect to different parameters are calculated; indicating parameters that are important for control strategies to bring R0 below 1, the effective contact rate β has the largest elasticity index. As the disease control options are associated to these parameters, for some values of these parameters, R0 &lt; 1, thus the disease can be controlled.

Perceptions, circumstances and motivators affecting the implementation of contagious bovine pleuropneumonia control programmes in Nigerian Fulani pastoral herds

Contagious bovine pleuropneumonia (CBPP) is an infectious disease of cattle which substantially contributes to poor productivity of the sub-Saharan pastoral livestock sector. In Nigeria and most of the West African countries, limited public funding for CBPP control have necessitated farmers to bear a bigger burden of managing the disease. Understanding the factors influencing decision of farmers to implement disease control programmes is therefore a key element in informing future policies aimed at improving CBPP management. This study explored perceptions of Nigerian Fulani pastoral herdsmen on the responsibility for cattle healthcare, and identified their circumstances and motivations in implementing CBPP management programmes. Field data were collected from 191 pastoral farmers using a semi-structured, interviewer-administered questionnaire. The results indicated that younger farmers were more likely than their older counterpart to accept the responsibility for CBPP management (p<0.01). This may signal future prospects for improved CBPP management where upcoming farmers could be encouraged to implement CBPP control programmes and uphold the costs. 13.6% of the farmers had no intention of implementing any CBPP control programme on farm, while 81.2% either had a positive intention or implemented at least one programme aimed at controlling CBPP. Intention to implement CBPP control programmes was significantly associated with educational attainment of farmers (p<0.01) and their access to CBPP control services offered by trained veterinarians (p<0.01). Farmers with negative attitudes towards implementing CBPP control programmes could be motivated to change their perspectives by advice from trusted sources and improved access to veterinary services. Conversely, farmers with positive attitudes towards implementing CBPP control programmes were more likely to be motivated by affordable veterinary services and advocacy on specific CBPP control programmes. As such, the former group of farmers will be more likely to benefit from programmes which focus on providing credible information from trusted sources, such as extension agents, veterinarians or successful peers. On the contrary, interventions targeting the latter group of farmers should prioritize cost-effective delivery of improved CBPP control technologies.

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No2016/429): contagious bovine pleuropneumonia.

Contagious bovine pleuropneumonia has been assessed according to the criteria of the Animal Health Law (AHL), in particular criteria of Article 7 on disease profile and impacts, Article 5 on the eligibility of contagious bovine pleuropneumonia to be listed, Article 9 for the categorisation of contagious bovine pleuropneumonia according to disease prevention and control rules as in Annex IV and Article 8 on the list of animal species related to contagious bovine pleuropneumonia. The assessment has been performed following a methodology composed of information collection and compilation, expert judgement on each criterion at individual and, if no consensus was reached before, also at collective level. The output is composed of the categorical answer, and for the questions where no consensus was reached, the different supporting views are reported. Details on the methodology used for this assessment are explained in a separate opinion. According to the assessment performed, contagious bovine pleuropneumonia can be considered eligible to be listed for Union intervention as laid down in Article 5(3) of the AHL. The disease would comply with the criteria as in Sections 4 and 5 of Annex IV of the AHL, for the application of the disease prevention and control rules referred to in points (d) and (e) of Article 9(1). The animal species to be listed for contagious bovine pleuropneumonia according to Article 8(3) criteria are species of the family Bovidae as susceptible.