Consumption Of Raw Meat Research Articles

Molecular detection of Toxoplasma gondii infection in slaughtered ruminants (sheep, goats and cattle) in Northwest Tunisia

The present study aimed to estimate the molecular prevalence of T. gondii infection in meat from slaughtered sheep, goats and cattle in Northwest Tunisia (Béja district). PCRs were performed on genomic DNA extracted from 420 meat samples (150 ewes, 120 goats and 150 cows). The overall molecular prevalence of T. gondii in sheep, goats and cattle were 33.3 (50/150), 32.5 (39/120) and 19.3% (29/150), respectively. Toxoplasma gondii molecular prevalences in the three meat ruminant species were significantly higher in adults compared to young animals (p<0.001). The infection prevalence differed significantly within localities in sheep (p<0.001), goats (p<0.001) and cattle (p=0.019). These results provide important information about exposure of humans to T. gondii through the consumption of raw or undercooked ruminants' meat. Extension programmes should be implemented to decrease the risk of infection related to sheep, goats and cattle meat manipulation and raw or undercooked meat consumption.

Seroepidemiology of Toxoplasma gondii infection in patients with liver disease in eastern China.

The role of the protozoan parasite Toxoplasma gondii in the pathogenesis of liver disease has recently gained much interest. The aim of this study was to determine the prevalence and risk factors associated with T. gondii infection in patients with liver disease from three cities in Shandong and Henan provinces, China. A case-control study was conducted from December 2014 to November 2015 and included 1142 patients with liver disease and 1142 healthy controls. Serum samples were collected from all individuals and were examined with enzyme-linked immunosorbent assay for the presence of anti-T. gondii IgG and IgM antibodies. Information on the demographics, clinical, and lifestyle characteristics of the participants was collected from the medical records and by the use of a questionnaire. The prevalence of anti-T. gondii IgG was 19·7% in patients with liver disease compared with 12·17% in the controls. Only 13 patients had anti-T. gondii IgM antibodies compared with 12 control individuals (1·14% vs. 1·05%, respectively). The highest seroprevalence was detected in patients with liver cancer (22·13%), followed by hepatitis patients (20·86%), liver cirrhosis patients (20·42%), and steatosis patients (20%). Multivariate logistic regression analysis indicated that consumption of raw meat (odds ratio (OR) = 1·32; 95% confidence interval (CI) 1·01-1·71; P = 0·03) and source of drinking water from wells (OR = 1·56; 95% CI 1·08-2·27; P = 0·01) were independent risk factors for T. gondii infection in liver disease patients. These findings indicate that T. gondii infection is more likely to be present in patients with liver disease. Therefore, efforts should be directed toward health education of populations at high risk of T. gondii infection and measures should be taken to protect vulnerable patients with liver disease.

The survey of internal parasites of consumed - Siamese cobra (Naja kaouthia) in Thailand.

In Asia and Africa, snake meat and organs are commonly prepared for consumption as traditional medicine or aphrodisiac regardless of the awareness of zoonotic diseases. Parasitic zoonosis can be contracted from the consumption of raw reptile and amphibian meat. Therefore to study this further, forty wild-caught Siamese cobras (Naja kaouthia) which were supplied to exotic restaurants in Thailand were randomized for fecal and blood examination as well as necropsy to dissect for the presence of parasites. Six groups of internal parasites and one blood parasite were found. The prevalence of rhabditids, Kalicephalus spp. and pentastomids were 82.5% (32/40), 60% (24/40) and 22.5% (9/40), respectively. Pentastomids are one of the potential zoonotic parasites reported in Thailand and other countries. But there is no report of pentastomids in Siamese cobra. Therefore, the further species identification of pentastome should be performed to raise community awareness on snake consumption.

Brucellosis in Chile: Description of a series of 13 cases

Brucellosis is a zoonosis caused by Brucella spp. It may be acquired by consuming unpasteurized dairy products. Brucellosis has a low incidence in Chile, thus, we have a scarce data. To report and to characterize the first series of clinical cases of adult patients diagnosed with brucellosis in Chile. We describe a series of 13 clinical cases in patients diagnosed between 2000 and 2016 in three different centers in the Metropolitan Region, Chile. A retrospective analysis was performed on clinical presentation, laboratory, antibiotic treatment, morbidity and mortality. The mean age was 50 years old. Eight cases had a record of consumption of unpasteurized dairy products. The most frequently reported complaints were fever. The most frequent focal point involved was the spine. Only one patient had a positive blood culture, while the diagnosis was made using serological techniques in the other part of the group. The most indicated antibiotic regimens were doxycycline-rifampicin and doxycycline-gentamicin. The hospital stay was 20 days approximately as an average. Clinical cure was achieved in all cases. Brucellosis is an infrequent zoonosis in Chile, and it produces a nonspecific clinical picture, so it is necessary to have high suspicion to make the diagnosis based in the antecedent of consumption of unpasteurized dairy or raw meat.

Mycobacterium tuberculosis Infection in a Domesticated Korean Wild Boar (Sus scrofa coreanus)

Tuberculosis, a chronic progressive disease, has been reported in bovine, swine, and primate species. Here, we report the first case of Mycobacterium tuberculosis infection in a Korean wild boar ( Sus scrofa coreanus). The owners this domesticated boar brought it to the Gyeongbuk Veterinary Service Laboratory in Korea after it was found dead and severely emaciated. Demarcated yellowish white nodules were found around the larynx and retropharyngeal lymph node during necropsy. The lungs had diffuse fibrinous pleuritis, severe congestion, and scattered nodules. More nodules were found in the spleen. Tuberculosis is characterized by massive macrophage infiltration and central caseous necrosis; both characteristics were found in the lungs. Histopathologic examination revealed that the alveolar lumen had marked fibrosis and exudates. Examination of the fluid revealed extensive macrophage permeation. To confirm a Mycobacterium infection, PCR was performed using two primer sets specific to the rpoB gene of Mycobacterium; Mycobacterium was detected in the lungs and spleen. To identify the species of Mycobacterium, immunohistochemical evaluation was performed using antibodies against Mycobacterium tuberculosis and Mycobacterium bovis . The results revealed immunoreactivity against M. tuberculosis but not against M. bovis . The consumption of undercooked or raw meat from game animals may expose humans and other animals to sylvatic infection. Consequently, Koreans who ingest wild boar may be at risk of a tuberculosis infection. To reduce the risk of foodborne infection and maintain public health, continuous monitoring and control strategies are required.

Toxoplasma gondii in the Food Supply.

Toxoplasmosis is caused by infection with the protozoan parasite Toxoplasma gondii. Infections are usually either asymptomatic or develop mild symptoms that are self-limited, but infections in immunosuppressed persons can be severe. Infections in pregnant women can cause serious health problems in the child such as mental retardation and blindness. Infection with T. gondii in immunocompetent adults can lead to impaired eyesight. Toxoplasmosis has ranked very highly in two studies of death and disability attributable to foodborne pathogens. The consumption of raw or undercooked meat containing T. gondii tissue cysts and the consumption of raw vegetables or water contaminated with T. gondii oocysts from cat feces is most frequently associated with human illness. The risk of acquiring a Toxoplasma infection via food varies with cultural and eating habits in different human populations.

Seroprevalence and molecular epidemiology of EAST1 gene-carrying Escherichia coli from diarrheal patients and raw meats.

Several Escherichia coli pathotypes have been reported in Thailand; however, information on enteroaggregative heat-stable enterotoxin 1 (EAST1)-carrying E. coli (EAST1-EC) is insufficient. Previous reports show that consumption of raw meats causes diarrheagenic E. coli infections. In this study, we investigated the seroprevalence and genetic relationship of EAST1-EC from clinical and raw meat samples. Diarrheal patients and raw meat samples were investigated for the presence of EAST1-EC by performing polymerase chain reaction (PCR) to detect astA. Serotyping, antimicrobial susceptibility tests, and PCR-based phylogenetic group assay were performed. Molecular epidemiology of E. coli strains from clinical and raw meat samples was determined using repetitive element-PCR typing, BOX-PCR, and ERIC2-PCR. Results showed that 11.2% (17/152) of clinical samples and 53.3% (16/30) of raw meat samples had EAST1-EC. In all, 24 and 36 EAST1-EC strains were successfully isolated from 17 clinical and 16 raw meat samples, respectively. These strains had astA but did not possess the indicative genes of other E. coli pathotypes and were therefore classified as EAST1-EC. Most of these strains were multidrug resistant and were classified into nine serogroups. Molecular genotyping showed identical DNA fingerprint among EAST1-EC serotype O15 strains from clinical and raw chicken samples, suggesting that they were derived from the same bacterial clone. Our results indicated a high prevalence of multidrug-resistant EAST1-EC strains in clinical and environmental samples in Thailand belonging to nine serogroups. Moreover, the study highlighted the close association between infections caused by EAST1-EC serotype O15 and raw meat consumption.

Risk Analysis and Occurrence of Hepatitis E Virus (HEV) in Domestic Swine in Northeast Brazil.

Anti-HEV antibodies were detected in animals from abattoir and in farms from northeast Brazil. Our results suggest that HEV is highly disseminated in the swine population and might present a great risk to animal handlers and for consumption of raw or undercooked meat and meat products in northeast Brazil.

Magnetic Stirrer Method for the Detection of &lt;em&gt;Trichinella&lt;/em&gt; Larvae in Muscle Samples

Trichinellosis is a debilitating disease in humans and is caused by the consumption of raw or undercooked meat of animals infected with the nematode larvae of the genus Trichinella. The most important sources of human infections worldwide are game meat and pork or pork products. In many countries, the prevention of human trichinellosis is based on the identification of infected animals by means of the artificial digestion of muscle samples from susceptible animal carcasses. There are several methods based on the digestion of meat but the magnetic stirrer method is considered the gold standard. This method allows the detection of Trichinella larvae by microscopy after the enzymatic digestion of muscle samples and subsequent filtration and sedimentation steps. Although this method does not require special and expensive equipment, internal controls cannot be used. Therefore, stringent quality management should be applied throughout the test. The aim of the present work is to provide detailed handling instructions and critical control points of the method to analysts, based on the experience of the European Union Reference Laboratory for Parasites and the National Reference Laboratory of Germany for Trichinella.

Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples.

Trichinellosis is a debilitating disease in humans and is caused by the consumption of raw or undercooked meat of animals infected with the nematode larvae of the genus Trichinella. The most important sources of human infections worldwide are game meat and pork or pork products. In many countries, the prevention of human trichinellosis is based on the identification of infected animals by means of the artificial digestion of muscle samples from susceptible animal carcasses.There are several methods based on the digestion of meat but the magnetic stirrer method is considered the gold standard. This method allows the detection of Trichinella larvae by microscopy after the enzymatic digestion of muscle samples and subsequent filtration and sedimentation steps. Although this method does not require special and expensive equipment, internal controls cannot be used. Therefore, stringent quality management should be applied throughout the test. The aim of the present work is to provide detailed handling instructions and critical control points of the method to analysts, based on the experience of the European Union Reference Laboratory for Parasites and the National Reference Laboratory of Germany for Trichinella.

Predictors of Toxoplasma gondii infection in Czech and Slovak populations: the possible role of cat-related injuries and risky sexual behavior in the parasite transmission.

The protozoan Toxoplasma gondii infects about one-third of the world's population. The consumption of raw meat, contact with cats, contact with soil, and ingestion of food or water contaminated with soil are considered to be the most important sources of infection. Still in most women who were infected during pregnancy, no definitive source of infection is found. In 2014-2016, independent sources of T. gondii infection were searched for by gathering epidemiological data from 1865 (519 infected) responders. Touching garden soil (odds ratio (OR) 3·14, 95% confidence interval (CI) 1·3-6·35), sustaining cat-related injuries (OR 2·16, 95% CI 1·25-3·74), and eating improperly washed root vegetables (OR 1·71, 95% CI 1·02-2·87), but not risky sexual behavior (OR 1·22, 95% CI 0·79-1·90), were the predictors of infection. The seroprevalence of T. gondii infection had been increasing up to ages 35-50 in men and ages 50-54 in women. Past those ages, seroprevalence of toxoplasmosis has been decreasing. This suggests that the natural decrease of anamnestic antibodies concentrations over time leads to positivity-to-negativity seroconversion in many subjects. If this is true, then the prevalence of T. gondii infection in a general population and its potential impacts on public health could be much larger than generally believed.

Seroprevalence of Toxoplasma gondii in hunted wild boars (Sus scrofa) from southeastern France.

Toxoplasma gondii (Nicolle et Manceaux, 1908) is an obligate intracellular, parasitic protozoan within the phylum Apicomplexa that causes toxoplasmosis in mammalian hosts (including humans) and birds. Since meat of wild boar, Sus scrofa (Linnaeus), has been demonstrated to be a potential source of human infection, a careful evaluation of the prevalence of infection with T. gondii in hunted animals is needed to protect public health. In the Var area in southeastern France, we performed a spatio-temporal survey in order to investigate the prevalence of IgG antibodies in wild boars shot by hunters in the Canjuers military camp during two subsequent hunting seasons. Of 841 wild boars screened, antibodies (IgG) to T. gondii (modified agglutination test, cut-off 1 : 6) were found in 141 (16.8%) muscle extract samples. A significant association (p < 0.001) was found between positivity and age, but not gender, and hunting districts. The results obtained indicate that consumption of raw or undercooked meat from wild boars carries an important risk of infection with T. gondii. Wild boars may be considered as a bioindicator of parasite circulation in this ecosystem.

Retrospective studies of abattoir zoonoses in Nigeria: public health implications.

Abstract Abattoirs are major sources of valuable information for animal disease outbreaks, but also an environment for transmission of meat-borne zoonoses, which may constitute a major threat to public health and food safety. This study aims to identify common abattoir zoonoses, the geographic distribution and the risk factors predisposing to their spread across Nigeria; and also to evaluate the prevalence estimates of abattoir zoonoses in human and slaughter animals. A systematic search of regional databases based on set criteria, yielded 47 relevant articles scrutinized critically to find the best available evidences to support the study purpose. All the publications which cut across the six geo-political regions of Nigeria carried out confirmatory diagnosis of the causative organisms. The results yielded 11 human and 53 slaughter animal causal organisms of abattoir zoonoses and obligate pathogens of human infections. They constitute bacterial (68.2%), helminths (21.2%), viral (6%) and protozoan (4.6%) due to infection or contamination. There was an increasing trend in occurrence of abattoir zoonoses as the year of studies progressed; also there were corresponding increase in human cases of abattoir zoonoses as the occurrence in slaughter animals' increased from 2001 to 2016. Some risk factors identified for the human abattoir zoonoses include; butchers with open wounds, occupational exposure of greater than 5 years and consumption of raw meat. Abattoir workers are at higher risk of being exposed to abattoir zoonoses due to occupational hazards; also consumer's choice of meat is significantly associated with the type of meat. Abattoir zoonoses exist in Nigeria, but the occurrence, spread and prevalence can be mitigated through proper meat inspection, improved hygiene and creating greater awareness of risks and avoidance.

Seroprevalence and Potential Root Cause of Toxoplasmosis in Primary Children : A Study Conducted at King Abdulaziz Hospital

Background: Toxoplasmosis is a zoonosis with a high prevalence throughout the world. Toxoplasma gondii infection is frequently asymptomatic. Primary school children are particularly vulnerable to toxoplasmosis due to their habits of playing in water, soil, eating various raw foods, or contact with pets, including dogs, cats, and birds and hence they are an ideal target group to investigate T. gondii prevalence. Data collected from this age group can thus be used to assess whether T. gondii threatens the health of school-aged children, and also as a reference for evaluating the need for community interventions. The aim of this study was to determine the prevalence rate of anti-toxoplasmosis IgG and IgM in Primary School children and to detect agents that increase prevalence of the disease. Materials and methods: 328 primary children (219 females and 109 males) were screened for T. gondii antibodies with ELISA along with a questionnaire conducted to all children and parents/guardians to obtain data for relevant eating and social habits. Among the risk factors tested, including contact with cats and soil, consumption of raw meat and vegetables, and drinking unboiled milk. Results: the seroprevalence of anti-Toxoplasma IgG & IgM was found to be 12 % (40 out of 328) and 2.4 % (8 out of 328), respectively while combined anti Toxoplasma IgG & IgM antibodies were found in 1.5 % of the children (5 out of 328). Infection acquisition rate was directly proportional to age and it was of interest that the most significant risk factor was not the petting of cats but the ingestion of raw meat. The seroprevalence of T. gondii IgG & IgM by ELISA among primary children in this region of Saudi Arabia is considerable with few identifiable significant risk factors reported. Conclusion: effective measures should be taken to prevent and control T. gondii infection in primary school children, the results of this study showed that in order to increase awareness of the disease in the community especially for girls and their parents on common root causes such as raw meat and vegetable is necessary. Policy makers also need to initiate prevention and control programs to not only primary children but also pregnant women and immunocompromised patients in particular because they are more severely affected by T. gondii infection.

Seroprevalence and Associated Factors of Toxoplasma gondii Infection among Pregnant Women Attending in Antenatal Clinic of Arba Minch Hospital, South Ethiopia: Cross Sectional Study

Background: Toxoplasma gondii (T. gondii) is a ubiquitous, coccidian intracellular protozoan parasite that causes toxoplasmosis. T. gondii infection acquired during pregnancy may result in severe damage or death of the fetus and long-term sequelae in offspring. So far, no documented data concerning the seroprevalence of T. gondii infection among pregnant women is available in the study area. Therefore, this study was aimed at determining of T. gondii sero-prevalence and associated factors among pregnant women attending in ante natal clinic (ANC) of Arba Minch hospital, southern Ethiopia. Methods: A facility based cross sectional study design was employed. A total of 232 pregnant women visiting Arba Minch hospital ANC from February to April, 2015 were enrolled by using systematic sampling technique. Data regarding Socio-demographic and associated factors were gathered using pretest questionnaire. Approximately 2 milliliters of blood specimen was collected and serum samples were tested for anti-T. gondii IgG and IgM antibody using Enzyme Linked Immunosorbent Assay. Data were analyzed using SPSS version 20. Results: Out of the total 232 pregnant women tested, 184 were found to be seropositive, giving overall seroprevalence rate of T. gondii infection 79.3% (95%CI; 73.7-84.5). On multivariate analysis showed that consumption of raw meat (AOR=3.211; 95% CI: 1.592-6.477) and habit of eating raw vegetables or fruit (AOR=2.669; 95% CI: 1.264-5.639) were significantly associated with T. gondi infection. Conclusion: The overall seroprevalence of T. gondii infection among pregnant women was high and serological evidence of primary infection was observed. Therefore, screening of Toxoplasma infection should be considered during ANC follow up. Moreover, health information about ways to minimize exposure to the risk factors should be provided with main focus on not eats raw meat and raw vegetables.

Comparing the Clinical Features and Outcomes of Acute Hepatitis E Viral Infections with Those of Acute Hepatitis A, B, and C Infections in Korea

Objectives: This study investigated the etiology of acute viral hepatitis and compared the clinical features of hepatitis E virus (HEV) infections with those of other acute viral hepatitis infections in Korea. Methods: This study included 2,357 consecutive patients who were diagnosed with acute hepatitis, based on acute illness with jaundice or elevated alanine aminotransferase levels (>100 IU/L), between January 2007 and January 2016. Acute viral infections were observed in 23 (19.8%) patients with HEV, 49 (42.2%) patients with hepatitis A virus, 28 (24.1%) patients with hepatitis B virus, and 16 (13.8%) patients with hepatitis C virus. Results: The incidence of acute HEV infection was higher among older patients (median age: 49 years) and male patients (69.6%), and was associated with the consumption of undercooked or uncooked meat (43.5%). Half of the acute HEV infections involved underlying liver disease, such as alcoholic liver disease, chronic hepatitis B, common bile duct stones, and autoimmune hepatitis. Two HEV-infected patients were diagnosed with Guillain-Barré syndrome, although no patients developed fulminant hepatitis. Conclusion: Our findings indicate that HEV infection in Korea is frequently transmitted through the consumption of raw meat and may cause acute or chronic liver disease.

The Bacteriological Quality, Safety, and Antibiogram of Salmonella Isolates from Fresh Meat in Retail Shops of Bahir Dar City, Ethiopia.

The habit of raw meat consumption in addition to the poor hygienic standards and lack of knowledge contribute to food-borne diseases outbreaks. The objective of this research was to assess the bacterial quality and safety of fresh meat from retail Bahir Dar City, Ethiopia. A total of 30 fresh meat samples were collected from butcher shops. Standard bacteriological methods were used to isolate and enumerate bacteria. Kirby-Bauer disk diffusion method was used for antimicrobial susceptibility testing of Salmonella isolates. The mean counts of AMB, TC, and S. aureus were log104.53, 3.97, and 3.88 log10cfu/g, respectively. Salmonella was isolated from 21 (70%) of the samples. Salmonella isolates in this study were highly susceptible to ciprofloxacin, gentamycin, and norfloxacin while they were resistant to erythromycin and tetracycline. High rate of multiple drug resistance was also noticed in Salmonella isolates. The microbial loads of meat were above the recommended microbial safety limits. Besides this, the isolation rate of Salmonella was high and high levels of drug resistance were documented for Salmonella isolates. Measures on handling and appropriate personal hygiene practices of workers in the retail shops are recommended to reduce the change of forborne disease outbreaks.

Survival of Toxoplasma gondii tachyzoites in simulated gastric fluid and cow’s milk

The protozoan parasite Toxoplasma gondii is one of the most important food-related pathogens worldwide. Besides contact to oocysts or ingestion of tissue cysts mainly by consumption of raw or undercooked meat from infected animals, raw milk is considered to be a risk factor and possible route of transmission for tachyzoites. This stage of the parasite is usually very sensitive to acidic pH and, therefore, considered unlikely to survive stomach passage. However, tachyzoites were shown to survive for several days in milk and there are also reports on transmission of toxoplasmosis via milk. Thus, the aim of the study was to examine retention of infectivity of tachyzoites in simulated gastric fluid (SGF) of different acidity and to elucidate whether addition of different shares of milk would affect survival of the parasites. Tachyzoites were exposed to SGF of pH 2.0 through 6.0 and their remaining infectivity was examined by cell culture. Furthermore, the impact on survival was investigated in different admixtures of milk to the SGF (25, 50, 75%) as well as in pure milk. Tachyzoites were shown to retain infectivity in SGF of pH 5.0 and 6.0 for at least 90min while they were more sensitive to lower pH values. Admixture of milk resulted in extension of survival. The results support the hypothesis of tachyzoites to survive stomach passage and their retention of infectivity.

Parasitic zoonoses associated with consumption of raw fish meat

Brazil is one of the major world powers for fish-farming, with a fishing and aquaculture production volume reaching more than one million tons in 2004. National Aquaculture production is growing on average 21.1% annually, surpassing statistically other activities of national importance such as cattle farming. However the per capita consumption is low, about 6 kg per year due to the high cost of the final product and eating habits that value the consumption of bovine meat. The meat of the fish is a source of high biological value protein, and is rated as nutritionally beneficial to human health by being an important source of essential aminoacids, vitamins, minerals and fatty acids. The fish can be marketed as fresh, chilled or frozen, the first one being the national preference. Inspection of the product to be marketed should be carefully observed, evaluating the organoleptic characteristics and presence of parasites found in the flesh. When the fish are cultivated, so are all sea food creatures, are subjected to factors that lead to stress and as a consequence may arise concomitant diseases, particularly parasitic diseases, which are a risk to public health since some of these parasites cause zoonosis. The increasing incidence of parasitic zoonosis are associated with the consumption of raw fish or insufficiently cooked, and food habits that encourages ingestion of this type of meat, such as sushi and sashimi of eastern culture. The main parasitic zoonosis is transmitted by this inadequate consumption are anisakiasis, eustrongiliase the capilariase the fagicolose the clonorquiose and diphyllobothriasis. Until this moment, there are few reports of parasitic diseases in humans in Brazil, probably due to lack of diagnosis and not by the absence of disease in the country. The sanitary inspection of products fish derivated is still scarce and studies concerning the importance of fish parasites in our country are reduced. Thus the best recommendation would be preventive abstinence from consumption of raw fish or meat not cooked properly, but heat treatment like cooking, is able to eliminate the parasite activity both in their adult and larval form, and freezing at -20 º C for seven days, or -35 º C for not less than fifteen hours, is also described as effective in eliminating these parasites. Health authorities, fish-farmers, veterinarians should seek greater control with regards to fish diseases of zoonotic character, from production to marketing of fish, to decrease the morbidity and mortality of the livestock, improve the quality of fish for consumers avoiding, for a prophylactic measure, the spread of zoonosis transmitted by fish.

Wild boar offal as a probable source of Aujeszky’s disease virus for hunting dogs in Poland

AbstractIntroduction:Aujeszky’s disease (AD), most often related to infection of domestic and feral swine, may also concern other mammals, including dogs. The disease in carnivores, related to consumption of raw meat or offal contaminated with AD virus, is manifested by severe neurological disorders and inevitably leads to animal’s death.Material and Methods:Karelian bear dog was euthanised due to nervous symptoms that started two days after participation in wild boar hunting. After exclusion of rabies the dog’s carcass was subjected to standard necropsy. Tissue samples were collected for histological examination. Samples of the brain were tested for ADV by real-time PCR and virus isolation. Samples of the liver were collected for toxicological examination.Results:The presence of ADV was confirmed by real-time PCR and virus isolation. Toxicological examination revealed anticoagulant poisoning. This is the first case of Aujeszky’s disease (AD) in a hunting dog in Poland after exposure to ADV from offal of wild boar.Conclusion:This infection should be taken into consideration in differential diagnosis of syndromes of neurological disorders in dogs. Since AD is found in both domestic pigs and wild boar in Poland, special care must be taken to prevent spread of infection to other species.