ObjectiveRadio Frequency Time Series (RF TS) is a cutting-edge ultrasound approach in tissue typing. The RF TS does not provide dynamic insights into the propagation medium; when the tissue and probe are fixed. We previously proposed the innovative RFTSDP method in which the RF data are recorded while stimulating the tissue. Applying stimulation can unveil the mechanical characteristics of the tissue in RF echo. Materials and methodsIn this study, an apparatus was developed to induce vibrations at different frequencies to the medium. Data were collected from four PVA phantoms simulating the nonlinear behaviors of healthy, fibroadenoma, cyst, and cancerous breast tissues. Raw focused, raw, and beamformed ultrafast data were collected under conditions of no stimulation, constant force, and various vibrational stimulations using the Supersonic Imagine Aixplorer clinical/research ultrasound imaging system. Time domain (TD), spectral, and nonlinear features were extracted from each RF TS. Support Vector Machine (SVM), Random Forest, and Decision Tree algorithms were employed for classification. ResultsThe optimal outcome was achieved using the SVM classifier considering 19 features extracted from beamformed ultrafast data recorded while applying vibration at the frequency of 65 Hz. The classification accuracy, specificity, and precision were 98.44 ± 0.20 %, 99.49± 0.01 %, and 98.53± 0.04 %, respectively. Applying RFTSDP, a notable 24.45 % improvement in accuracy was observed compared to the case of fixed probe assessing the recorded raw focused data. ConclusionsExternal vibration at an appropriate frequency, as applied in RFTSDP, incorporates beneficial information about the medium and its dynamic characteristics into the RF TS, which can improve tissue characterization.