We study half-BPS surface operators in mathcal {N}=2 supersymmetric asymptotically conformal gauge theories in four dimensions with SU(N) gauge group and 2N fundamental flavours using localization methods and coupled 2d/4d quiver gauge theories. We show that contours specified by a particular Jeffrey–Kirwan residue prescription in the localization analysis map to particular realizations of the surface operator as flavour defects. Seiberg duality of the 2d/4d quivers is mapped to contour deformations of the localization integral which in this case involves a residue at infinity. This is reflected as a modified Seiberg duality rule that shifts the Lagrangian of the purported dual theory by non-perturbative terms. The new rules, that depend on the 4d gauge coupling, lead to a match between the low energy effective twisted chiral superpotentials for any pair of dual 2d/4d quivers.