Confinement Stress Research Articles

Behavioural Monitoring Underlines Habituation to Repeated Stressor Stimuli in Farmed Gilthead Sea Bream (Sparus aurata) Reared at a High Stocking Density

A confinement stress test with 75% tank space reduction and behavioural monitoring through tri-axial accelerometers externally attached to the operculum was designed. This procedure was validated by demonstrating the less pronounced stress response in gilthead sea bream than in European sea bass (950–1200 g). Our study aimed to assess habituation to high stocking densities with such procedure in gilthead sea bream. Animals (420–450 g) were reared (June–August) in a flow-through system at two stocking densities (CTRL: 10–15 kg/m3; HD: 18–24 kg/m3), with natural photoperiod and temperature (21–29 °C), and oxygen levels at 5.2–4.2 (CTRL) and 4.2–3.2 ppm (HD). At the end, blood and muscle were sampled for haematology and transcriptomic analyses, and external tissue damage was assessed by image-based scoring. Four days later, fish underwent a 45 min confinement stress test over two consecutive days. HD fish showed reduced feed intake, growth rates and haematopoietic activity. Muscle transcriptome changes indicated a shift from systemic to local growth regulation and a primed muscle regeneration over protein accretion in HD animals with slight external injuries. After stress testing, HD fish exhibited a decreased recovery time in activity and respiration rates, which was shorter after a second stressor exposure, confirming habituation to high densities.

Evolution of Confinement Stress in Axially Loaded Concrete-Filled Steel Tube Stub Columns: Study on Enhancing Urban Building Efficiency

In the context of green building and sustainable urban development, understanding the mechanical behavior of structural components like concrete-filled steel tube (CFST) columns is crucial due to their improved load-bearing capacity, energy efficiency, and optimized material usage, which enhance structural resilience and sustainability. This research addresses the complex development of confining stress and its impact on the concrete core (CC) behavior within these columns, which are essential for urban infrastructure. Through extensive numerical studies, this study proposes a model to estimate the confining stress in axially loaded CFST short columns. Study findings reveal that the confinement effectiveness is influenced by variables such as compressive strength (CS) of the concrete, cross-sectional shape, and depth-to-wall thickness percentage. Additionally, the confinement is also significantly affected by the yield strain of steel εy/εc to the peak strain of unconfined concrete εc. A three-dimensional finite element model (FEM) was built for the simulation of the columns’ nonlinear behavior and was rigorously validated against experimental data. This model aids in the design and implementation of more efficient and resilient urban structures, supporting the principles of sustainable construction. The study underscores the importance of structural integrity in sustainable urban development and provides valuable insights for improving the design of green building materials.

Stress-strain modelling of circular concrete-filled FRP–steel composite tube columns under axial compression load

Concrete filled-circular steel tube (CFCST) columns, when externally confined with carbon or glass fiber-reinforced polymers (FRP) sheets and subjected to axial load, exhibit improved strength and ductility compared to unconfined cases. The mechanical properties of the confinement materials are crucial in determining the stress-strain relationship of the confined concrete. This study presents an in-depth analysis of the stress-strain behavior of FRP-confined CFST columns, based on data from 252 specimens. It examines a range of factors, including various infill concrete grades from 14.15 to 140.39 MPa, two types of carbon steel outer tubes with yield strengths (fy) from 226 to 466.5 MPa, and two types of FRP (CFRP and GFRP) with tensile strengths from 1260 to 4900 MPa. The complete axial stress-strain curve is divided into four phases according to a mathematical expression by Wu and Wei (2015): the elastic phase, nonlinear transition phase, linear softening phase, and residual phase. New formulas for ultimate stress, based on confinement stress, were proposed by Hassanin et al. (2023), while the ultimate strain was calculated using the classical formula by Richart et al. Peak stress and strain were determined through statistical linear regression analysis using SPSS software, based on 252 experimental data points and involved using a combined factor of steel contribution (Elsfco) and FRP contribution (Elffco). Eight specimens were specifically used to validate the models through Finite Element Method (FEM) simulations. The accuracy of the predicted analysis results was also assessed by comparing them to existing models. The evaluation showed that the models successfully simulated the complete stress-strain curve of FRP-wrapped CFST columns under axial load, producing satisfactory results. These four models provide reliable results for calculating ultimate stress, ultimate strain, peak stress, peak strain, and the complete stress-strain behavior of these columns.

Responses of oscar (Astronotus ocellatus) to different temperatures and its interaction with early mild stress

Non-optimum temperature results in stress in fish through different biological and physiological processes. This study investigated how early mild stress (EMS) and temperature can influence growth, hematology, blood biochemistry, immunological response, antioxidant system, liver enzymes, and stress response of juvenile oscar (Astronotus ocellatus; 8.02 ± 0.81 g) before and after acute confinement stress (AC stress). Eight experimental treatments were 17D (farmed at 17 °C), 22D (farmed at 22 °C), 27D (farmed at 27 °C), 32D (farmed at 32 °C), 17DES (farmed at 17 °C and EMS), 22DES (farmed at 22 °C and EMS), 27DES (farmed at 27 °C and EMS), and 32DES (farmed at 32 °C and EMS). At the end of the experiment (nine weeks), there was no significant difference in survival rate, feed conversion ratio, body composition, and survival rate after the AC stress (ten weeks). The growth rates in the 17 and 32 °C groups were lower than others, showing the negative effect of temperature being too high or too low. After nine weeks, fish farmed at 32 °C had the highest hematological parameters (hematocrit, hemoglobin, red blood cells, and blood performance) and liver enzymes (lactate dehydrogenase, alanine transaminase, and aspartate transaminase). This group also has the lowest lysozyme, alternative complement pathway (ACH50), immunoglobulin, complement C3, complement C4, superoxide dismutase, and glutathione peroxidase. The EMS did not positively or negatively affect fish physiology in this study. In conclusion, the optimum temperature for oscar to achieve the best growth performance, immune response, and health status is 22–27 °C.

Investigating the biological relevance of measuring gastrointestinal cortisol metabolite levels to assess stress responses in Atlantic salmon (Salmo salar L.) after an acute stress

Monitoring stress levels of farmed Atlantic salmon (Salmo salar) is important to ensure fish welfare and optimize farm operations. Feces could be a promising matrix for assessing stress responses in fish, based on their properties of low-invasive sampling and allowing repeated sampling over time. Meanwhile, elevated levels of cortisol metabolites (CMs) in feces indicate the increases in plasma cortisol levels (PLA) after exposure to acute stress. However, the dynamics of fecal CMs following acute stress in Atlantic salmon remain unclear. In this study, a confinement stress involving chasing and crowding was conducted to investigate the responses of gastrointestinal CMs to an acute stressor in Atlantic salmon. The post-smolts, with an average weight of 155.21 g, were sampled before and at 30 min, 1.5, 6, 12, 18, 24, 36, and 48 h after the onset of stress. Blood and gastrointestinal contents from the stomach, proximal intestine, and distal intestine of each fish were collected and subsequently analyzed, using competitive enzyme-linked immunosorbent assay (ELISA). The results demonstrated that the pre-stress level of PLA was low (4.28 ± 6.13 ng/ml) and reached a peak within 30 min following stress. The levels of CMs in gastrointestinal contents from stomach (SCMs), proximal intestine (PCMs), and distal intestine (DCMs) in pre-stress group were 0.82 ± 0.50, 18.31 ± 6.14 and 16.04 ± 6.69 ng/g, respectively. Gastrointestinal CMs increased significantly within 30 min and the peak levels of SCMs (3.51 ± 3.75 ng/g), PCMs (68.19 ± 23.71 ng/g) and DCMs (65.67 ± 23.37 ng/g) were found at 1.5 h post-stress. The significant increases in PCMs and DCMs post-stress validate the biological relevance of measuring intestinal CMs for assessing acute stress responses in Atlantic salmon. No significant difference was noted between PCMs and DCMs across all samples, suggesting that intestinal contents can serve as a suitable matrix compared with feces when measuring the responses of CMs to acute stress. The time lag between the peak of PLA levels and their reflection in the intestinal contents exceeded 1 h, indicating that using intestinal contents as a matrix to assess stress levels in fish can extend and delay the sampling window. This study highlights valuable guidance for determining the optimal times to utilize intestinal contents for measuring stress responses, providing further insights into the dynamics of fecal CM following acute stress.

Axial partial compressive behavior of round-ended concrete-filled steel tubular columns

The paper conducted 19 partially and 5 fully loaded tests on round-ended concrete-filled steel tubular (CFST) columns. These tests revealed that the ultimate strengths of round-ended CFST columns under partial load declined as the cross-sectional aspect ratio or partial compression area ratio increased. However, the use of a top plate effectively enhanced their ultimate strengths. In addition, a finite element model (FEM) was utilized to generate 487 large-size models to explore the confinement effect, stress distribution, and parametric effects. Since there are no specific design standards available for round-ended CFST columns, the paper has evaluated the applicability of design methods provided in Eurocode 4, AISC 360–16, and Chinese code GB50396 which are intended for circular, square, or elliptical CFST columns for round-ended CFST columns under partial load. However, these existing design techniques showed inaccurate and dispersed resistance predictions for round-ended CFST columns. Therefore, an effective simplified method for forecasting the resistance predictions of round-ended CFST columns under the partial load has been proposed based on the testing results and FEM analysis.

Candida spp. in Cetaceans: Neglected Emerging Challenges in Marine Ecosystems.

Cetaceans, which are crucial in marine ecosystems, act as sentinels for ecosystem and human-environmental health. However, emerging fungal infections, particularly by Candida spp., pose a growing concern in these marine mammals. This review consolidates current knowledge on the prevalence, clinical manifestations, species distribution, and antifungal resistance of Candida infections in cetaceans. We detail the diverse pathogenic impacts of Candida, including respiratory, dermal, and systemic afflictions, underscoring diagnostic and treatment challenges amid rising antifungal resistance. Our analysis extends beyond health concerns in captive cetaceans, where confinement stress heightens vulnerability, to encompass substantial ecological risks in wild populations. The review emphasizes the One Health perspective, linking cetacean health with broader environmental and human public health issues. We particularly focus on the potential zoonotic transmission of emerging fungal pathogens such as Candida auris and the role of environmental changes in fostering antifungal resistance. The study underscores the need for concerted, interdisciplinary efforts in veterinary, medical, and environmental sciences to enhance understanding and management of Candida infections in cetaceans. We advocate for comprehensive monitoring and collaborative research initiatives to mitigate the rising challenge of these infections. Addressing Candida spp. in cetaceans is not just a conservation priority but a critical step in safeguarding overall marine health and, by extension, human health in the context of evolving infectious diseases.

Adverse effects of excessive dietary arachidonic acid on survival, PUFA-derived enzymatic and non-enzymatic oxylipins, stress response in rainbow trout fry

Arachidonic acid (C20: 4n-6, AA) plays a fundamental role in fish physiology, influencing growth, survival and stress resistance. However, imbalances in dietary AA can have detrimental effects on fish health and performance. Optimal AA requirements for rainbow trout have not been established. This study aimed to elucidate the effects of varying dietary AA levels on survival, growth, long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic capacity, oxylipin profiles, lipid peroxidation, and stress resistance of rainbow trout fry. Over a period of eight weeks, 4000 female rainbow trout fry at the resorptive stage (0.12 g) from their first feeding were fed diets with varying levels of AA (0.6%, 1.1% or 2.5% of total fatty acids) while survival and growth metrics were closely monitored. The dietary trial was followed by an acute confinement stress test. Notably, while the fatty acid profiles of the fish reflected dietary intake, those fed an AA-0.6% diet showed increased expression of elongase5, highlighting their inherent ability to produce LC-PUFAs from C18 PUFAs and suggesting potential AA or docosapentaenoic acidn-6 (DPAn-6) biosynthesis. However, even with this biosynthetic capacity, the trout fed reduced dietary AA had higher mortality rates. The diet had no effect on final weight (3.38 g on average for the three diets). Conversely, increased dietary AA enhanced eicosanoid production from AA, suggesting potential inflammatory and oxidative consequences. This was further evidenced by an increase in non-enzymatic lipid oxidation metabolites, particularly in the AA-2.5% diet group, which had higher levels of phytoprostanes and isoprostanes, markers of cellular oxidative damage. Importantly, the AA-1.1% diet proved to be particularly beneficial for stress resilience. This was evidenced by higher post-stress turnover rates of serotonin and dopamine, neurotransmitters central to the fish's stress response. In conclusion, a dietary AA intake of 1.1% of total fatty acids appears to promote overall resilience in rainbow trout fry.

Mechanical analysis of axially-loaded SFRC columns reinforced by high-strength rebars: Testing and FE modelling

To fully utilise the excellent yield stress of the hot-rolled high-strength rebar (HSR) in concrete structures, it is of great necessity to improve the concrete's deformation capacity. Adding steel fibres is one of the leading ways to upgrade the ductility of cement-based material, yet investigation on the mechanical responses of the steel fibre concrete (SFRC) column reinforced with HSRs is rarely reported. To fill this gap, this paper conducts an experimental study to investigate the axial compressive performance of the SFRC-HSR columns. Based on the collected test data of actively and passively confined SFRC members, a modified concrete plasticity model is following developed and verified by comparing with the tests. The model includes a collection of modifications in the yield surface, flow rule and hardening law considering the influence of the fibre addition. In compliance with the detailed FE modelling settings, the confinement stress distribution and the cooperative working mechanism of the axially-loaded SFRC-HSR columns are revealed, and the reason for the difference in typical load-displacement curves is clarified. Finally, combined with the modified hardening rule and the yield model, stress-strain relationships of the concrete cover and core are proposed to predict the load-displacement development rule of the axially-loaded SFRC-HSR columns. This research could be a scientific reference for applying SFRC columns reinforced by HSRs in engineering practice.

Ab initio informed machine learning potential for tribochemistry and mechanochemistry: Application for eco–friendly gallate lubricant additive

Mechanochemistry and tribochemistry processes involve multiple physical/chemical interactions induced by extreme conditions including molecular confinement, high temperatures and mechanical stress applied. Simulating these processes by molecular dynamics is very challenging. While force fields fall short reproducing the enhanced reactivity arising by quantum effects, ab initio molecular dynamics is severely limited by the complexity of the systems of interest, their sizes, and the long-time scale on which relevant events take place. In this work using an active learning approach, a landmark deep neural network potential has been developed which reproduces the accuracy of ab initio interactions at the classical molecular dynamics computational cost and permits to successfully simulate the tribochemical processes occurring at the interface between butyl gallate molecules and iron substrates under tribological conditions. The simulations of the dynamics of the Fe-gallate system when sliding under an imposed external load reveal the key atomistic mechanisms underlying the formation of the friction reducing lubricant tribofilm and permit to characterize the tribological properties of the explored systems, clearly exposing the shortcoming of reactive force field based approaches. The successful development of neural network potentials, making it possible to push the limits of molecular dynamics marrying accuracy with system sizes and long time scales, paves the route toward a new area in computational tribochemistry.

Experimental study of the shear behavior of concrete-rock interfaces under static and dynamic loading in the context of low confinement stress

Modified static and dynamic shear tests are introduced to study the shear behavior of concrete-rock interfaces under static and dynamic loading in the context of low confinement stresses. Static and dynamic shear tests of concrete-sandstone and concrete-granite interfaces are performed using these techniques. Three levels of interface roughness are considered: smooth, bush-hammered, and rough rock surfaces. The results of these tests show that in both static and dynamic regimes, the shear evolution of concrete-rock interfaces can be described according to three successive stages: the shear stress accumulation, the shear slip, and the residual shear stress stage. The main parameters driving the shear process are the concrete-rock bonds, the interface roughness, and the residual friction. However, unlike in the static shear evolution, in the dynamic shear evolution, the concrete-rock bonds and the roughness seem active in the shear stress accumulation stage. Furthermore, the correlation between the shear strength and the normal stress is stronger in static than dynamic conditions. The significance of the normal stress on the dynamic shear strength appears more important in rough concrete-granite interfaces than in the other two interfaces. Lastly, the dynamic peak shear strengths of all the interfaces tested are three to four times higher than their static counterparts.

Uniaxial tensile behavior of half-grouted sleeve connections with concrete cover after exposure to elevated temperatures

This study presents the tensile behavior of a half-grouted sleeve connection (HGSC). A total of 15 prism samples of grout are subjected to a series of tests before and after exposure to various temperatures. A total of 75 eccentric HGSC specimens with a 40 mm concrete cover and 15 central HGSC specimens without a concrete cover were fabricated for uniaxial tensile loading tests. A pseudo-transient heating regimen was applied to HGSC specimens with concrete covers; the specimens were heated to the desired temperature and left there for a duration of 0 min. This study systematically investigates the tensile properties of post-fire HGSC specimens to assess the combined effects of peak environmental temperature (20 °C, 200 °C, 400 °C, 600 °C, 800 °C), concrete cover depth (0 mm, 40 mm), concrete strength grade (C30, C35, C40, C45, C50), and concrete spalling (burst or unburst). The failure mode, load–displacement curves, sleeve strain distribution, and heat transfer are employed to characterize the response of the HGSC specimens, which is subsequently discussed. It is discovered that, although concrete compressive strength plays a minor role, temperature, concrete cover, and explosive spalling significantly influence certain post-fire tensile parameters of the HGSC. Based on the results, there is considerable potential for determining the ultimate tensile force in HGSC specimens with concrete covers and the remaining compressive strength of grout using ultrasonic pulse velocity detection. Furthermore, a comparative accuracy analytical model, incorporating the influence of tangential strain derived from the experimental results, is established based on confinement stress.

Triaxial mechanical behaviours and life cycle assessment of sustainable multi-recycled aggregate concrete

Multi-recycling of concrete waste presents a promising avenue for carbon-negative development and a circular economy. This study comprehensively assesses the triaxial mechanical performance and environmental impact of multi-recycled concrete (Multi-RAC) through three recycling cycles. The results reveal a triaxial failure mode similar to natural aggregate concrete (NAC). The peak stress and peak strain monotonically increase with confinement stress, showing a significant impact (enlarged by 171.4 % to 280.6 % and 397.4 % to 412.0 %, respectively) from 0 to 20 MPa. All P-values for recycling cycles and confining pressure are less than 0.05, with the confining pressure having a more significant effect. Three best-fit multivariate mixed models predict mechanical properties, and a modified elastoplastic model introduces the recycling cycles factor. Numerical simulations confirm the model's accuracy in predicting the triaxial mechanical properties of Multi-RAC. Comparative analysis reveals that the elastoplastic model-derived non-integral high order failure criterion outperforms the Willam-Warnke failure criterion and other conventional criteria. Regarding environmental impact, all indicators (GWP, POCP, AP, EP, and CED) decrease favourably with the increasing number of recycling cycles, with CED and EP playing the most significant roles. Compared to NAC, the five environmentally favorable indicators for RACIII decrease by 3.24 % to 50.6 %, respectively. These findings provide valuable insights for future research on developing eco-friendlier Multi-RAC for sustainable and green infrastructure.

The crosstalk between photoperiod and early mild stress on juvenile oscar (Astronotus ocellatus) after acute stress.

Early mild stress (EMS) is like preparedness and might help fish deal with stress appropriately. This study investigated how EMS and photoperiod changes can impact growth, haematology, blood biochemistry, immunological response, antioxidant system, liver enzymes, and stress response of oscar (Astronotus ocellatus; 7.29 ± 0.96 g) before and after acute confinement stress (AC stress). Ten experimental treatments included five different photoperiods 8L16D (08:16 light to dark), 12L12D (12:12 light to dark), 16L8D (16:08 light to dark), 20L4D (20:04 light to dark), and 24L0D (24:00 light to dark), and these five photoperiod schedules were conducted in an EMS condition. After 9 weeks, no significant differences were found in growth parameters, survival rate, and body composition. At the end of the experiment and after AC stress, fish farmed in 24 light hours had the lowest haematocrit, white blood cells, total protein, blood performance, lysozyme, immunoglobulin M, complement C3, superoxide dismutase, and catalase. Fish that experienced EMS had significantly higher survival rates than those farmed in normal conditions (80.67% vs 61.33%). In conclusion, considering all measured parameters, 8-h light can be suggested as an optimum photoperiod for this fish species. Under 24L0D (no EMS) conditions, there were many negative effects apparent. In addition, a positive effect of EMS was evident in terms of survival after AC stress. AC stress decreased some health parameters under 24-h light treatment, while these results were not observed in EMS-exposed fish. Therefore, the EMS schedule can be a useful tool in preventing the negative effects of stress.

Loess susceptibility to erosion: Interaction of cohesion sources, air slaking and confinement

AbstractErosion in loess is a widespread phenomenon, as loess covers about 10% of the Earth's land surface. While erosion of loess soil has been intensively studied, the mechanisms controlling erosion in gullies and pipes in loess are poorly understood. Cohesion plays an important role in erosion in loess. Interactions between erosional processes and stabilizing mechanisms in loess are poorly understood. This study focuses on the interaction between air slaking and stabilization by confinement to improve leaching techniques for identifying cohesion sources in undisturbed loess from four sites in Czechia. The decrease in tensile strength of samples after leaching in distilled water, dithionate, HCl, and hydrogen peroxide was used to selectively remove cohesion sources such as Fe‐Al oxides and hydroxides, carbonates, and organic matter. Experiments showed that confinement and overburden stress are important but neglected stabilizing mechanisms in loess. Leaching of unconfined loess samples gave misleading results because of ubiquitous air slaking. To reliably identify cohesion sources, samples confined in compacted sand were used. Leaching of confined samples showed that Fe‐Al oxides and hydroxides are major sources of cohesion (60–90%), while carbonates and organic matter are of minor importance (0–30%). To avoid misleading results, examination of loess structure after leaching is critical to identify samples with damaged structure due to enhanced air slaking caused by bubbles generated during leaching. Air slaking is a powerful and rapid damage mechanism, but it occurs only in dry or semi‐dry loess near the soil surface. In contrast, chemical weathering is able to remove cohesion sources in deeper parts of the loess profile.

When Mechanical Stress Matters: Generation of Polyploid Giant Cancer Cells in Tumor‐Like Microcapsules

AbstractBiofabrication techniques enable the performance of bioinspired three‐dimensional (3D) matrices resembling primary tumors. To validate their reliability, embedded cells may express complex biophysical responses. Among others, the emergence of tumor heterogeneity and the generation of Polyploid Giant Cancer Cells (PGCC), as a result of the mechanical stress, are two of the most challenging hallmarks to resemble in vitro. Here, these phenomena are studied in cells cultured on two‐dimensional (2D) flasks, in 3D spheroids, or immobilized within 3D polymer‐based tumor‐like microcapsules. These results show that cells cultured in 3D microcapsules exhibited an enhanced biomechanical heterogeneity, a higher number of PGCC, and an increased exertion of cell‐matrix attachment forces with respect to the other two experimental conditions. Additionally, cells isolated from tumor‐like microcapsules redistribute and align the cytoplasmatic protein Caveolin‐1, and upregulate markers involved in cell proliferation (i.e., Ki67), metastasis (i.e., TGF‐β1, TGF‐β‐R2), and epithelial to mesenchymal transition, to name a few. These hallmarks are barely described in the past as a result of the confinement and mechanical stress. Thus, in this work it is demonstrated that both the mechanical stress and confinement are required to stimulate cell polyploidy and biomechanical heterogeneity, which can be easily addressed by immobilizing breast cancer cells in tumor‐like microcapsules.

Comparison and Enhancement of Design Procedures for Guideline Expressions to Predict the Compressive Capacity of FRP-Wrapped Concrete Members

The FRP wrapping of reinforced concrete columns is a simple way to increase the axial load capacity of such members with substandard concrete quality. A total of 2197 specimens were collected as a database to discuss the efficiency of predicting axial compressive strength of FRP-wrapped concrete columns according to several national and international guidelines. Various aspects of the database, including fiber type, unconfined strength and specimen geometry used to investigate the guidelines, and results are presented in terms of averages, standard deviations, and ratio of overestimated specimens. The performance of these expressions was compared with one another based on these statistical results and graphs, and those that captured the behavior best were identified for specific subsets. Recognizing the expressions’ inadequacy in capturing efficiency improvements related to radius to area and confinement ratios, some improvements in the form of coefficients for lateral confinement stress in the Turkish Building Earthquake Code were introduced, specifically for certain subsets.

Plasma cortisol and production of miRNAs in red drum (Sciaenops ocellatus) exposed to three distinct challenges.

The red drum Sciaenops ocellatus is a marine fish species of high commercial interest. Despite improvements in current aquaculture practices, there are still concerns about the impact of daily manipulations regarding fish welfare. To investigate how does fish respond to various challenges, S. ocellatus juveniles were submitted to two acute challenges, namely a confinement stress and a cold-temperature shock, as well as a chronic stress challenge consisting of 18days of repetitive challenge events. The level of cortisol produced by individuals was used as a measure of activation hypothalamic-pituitary-interrenal (HPI) axis. A significant increase in cortisol levels was detected only after the confinement stress. Interestingly, the fish exposed to a chronic stress for 18days exhibited cortisol levels significantly lower than those of non-challenged fish. The small RNA-sequencing conducted for the chronic stress experiment only allowed us to identify two plasmatic microRNAs more abundant in non-challenged fish (miR-205-1-5p and let-7b-5p) compared to challenged fish. The miR-205-1-5p was of particular interest since it was already detected in previous studies on other fish species. In silico analysis allowed to predict potentially highly conserved mRNA targets of this specific miRNA, among which is tnfrsfa that plays a key role in the secondary stress response.

Confinement controls the creep rate in soft granular packings.

Flow in soft materials encompasses a wide range of viscous, plastic and elastic phenomena which provide challenges to modelling at the microscopic level. To create a controlled flow, we perform falling ball viscometry tests on packings of soft, frictionless hydrogel spheres. Systematic creep flow is found when a controlled driving stress is applied to a sinking sphere embedded in a packing. Here, we take the novel approach of applying an additional global confinement stress to the packing using an external load. This has enabled us to identify two distinct creep regimes. When confinement stress is small, the creep rate is independent of the load imposed. For larger confinement stresses, we find that the creep rate is set by the mechanical load acting on the packing. In the latter regime, the creep rate depends exponentially on the imposed stress. We can combine the two regimes via a rescaling onto a master curve, capturing the creep rate over five orders of magnitude. Our results indicate that bulk creep phenomena in these soft materials can be subtly controlled using an external mechanical force.

A machine learning based method for predicting the shear strength of Fiber-Reinforced Concrete joints in precast segmental bridges

The utilization of Fiber-reinforced concrete (FRC) in the construction of Precast segmental concrete bridges (PSCBs) is progressively increasing. The design of joints among precast members can considerably impact the overall integrity and safety of the structure. Consequently, accurately estimating the shear capacities of FRC joints has become critical for evaluating the safety of PSCBs structures. However, the effectiveness and efficiency of formulas or models intended for prediction of the shear capacities of joints with normal concrete are unknown in predicting the shear capacities of FRC joints. In this study, the machine learning (ML) algorithms are employed to predict the ultimate load of FRC joints, utilizing a database of 192 push-off test results. Three individual models (Lasso, SVM, and KNN) and three ensemble models (RF, GBDT, and XGBoost) were developed for estimating the ultimate load. The GridSearchCV technique was utilized for parameter optimization based on the training set, in conjunction with a five-fold cross-validation method. A comparative study revealed that the XGBoost ensemble model surpassed other models, with a predictive performance ranking as follows: XGBoost > GBDT > RF > KNN > SVM > Lasso. The best-performing XGBoost model achieved performance metrics of R2 = 0.964, MAE = 41.318 kN, RMSE = 56.760 kN, and MAPE = 14.228%. The superior reliability of the XGBoost ensemble model was further validated through comparisons with current design codes and existing models. As ML-based models are typically black-box models, the SHapley Additive exPlanations (SHAP) method was employed to explicitly link the predicted output to the inputs. Based on SHAP results, confining stress (CS), depth of shear key (D), compressive strength of concrete (fcu), total height of joints (H), and height of flat part (Hsm) recognized as the crucial parameters affecting ultimate load.