Expansion Conditions Research Articles

Optimization of T Cell Activation Protocols to Enrich Stem Cell Memory Phenotypes: Metabolic and Health Assays as Predictive Tools

Abstract The generation of T cells with a stem cell memory phenotype (T_SCM) holds significant promise for immunotherapy due to their longevity and potent antitumor activity. This study aimed to optimize T cell activation and expansion conditions to selectively enrich for the T_SCM population. Utilizing a suite of cell health and metabolism assays, we monitored T cell cultures to identify predictive markers of T_SCM enrichment. We observed that early activation steps correlated with a surge in ATP production and alterations in cell reducing potential, indicative of metabolic reprogramming. Subsequent activation phases were marked by an upregulation in both catabolic and anabolic pathways, as evidenced by the increased turnover of key metabolites including glucose, lactate, glutamine, glutamate, branched-chain amino acids (BCAA), and pyruvate. Notably, the rate of T cell activation influenced the composition of the resultant T cell populations, with the media composition further affecting these dynamics. Our findings suggest that metabolic assays, particularly those measuring changes in ATP levels and primary metabolite flux, can serve as effective early predictors of T_SCM enrichment. These insights may guide the development of more efficacious T cell-based immunotherapies by facilitating the targeted generation of T cells with a stem cell-like memory phenotype.

PENGEMBANGAN BAHAN PELATIHAN DESAIN SISTEM PEMBELAJARAN BAGI GURU BAHASA INDONESIA SMA

Purpose of this research is produce "Training Material of Study System Design for Senior High School Indonesia Language Teacher" as according (1) requirement of teacher; (2) condition of expansion; (3) effectif, (4) can increase teacher interest; and (5) earns for teacher training. This research adopts expansion models Dick and Carey (2009). The sequence: determination of general purpose, study analysis, identification of initial ability, writing of purpose of special, assessment expansion of directive reference, strategy expansion, election and matter expansion, scheme and expansion of formative evaluation, revision, and scheme and expansion of evaluation sumatif. Result of this research, in general shows product " Training Material", very good. Suggestion, that product exploited by Senior High School Indonesia Language Teacher and exploited for further education expansion. Keyword: Development, training materials, design, learning systems

Algorithms for the ferromagnetic Potts model on expanders

AbstractWe give algorithms for approximating the partition function of the ferromagnetic $q$ -color Potts model on graphs of maximum degree $d$ . Our primary contribution is a fully polynomial-time approximation scheme for $d$ -regular graphs with an expansion condition at low temperatures (that is, bounded away from the order-disorder threshold). The expansion condition is much weaker than in previous works; for example, the expansion exhibited by the hypercube suffices. The main improvements come from a significantly sharper analysis of standard polymer models; we use extremal graph theory and applications of Karger’s algorithm to count cuts that may be of independent interest. It is #BIS-hard to approximate the partition function at low temperatures on bounded-degree graphs, so our algorithm can be seen as evidence that hard instances of #BIS are rare. We also obtain efficient algorithms in the Gibbs uniqueness region for bounded-degree graphs. While our high-temperature proof follows more standard polymer model analysis, our result holds in the largest-known range of parameters $d$ and $q$ .

Unconditional convergence of eigenfunction expansions for abstract and elliptic operators

We study the most general class of eigenfunction expansions for abstract normal operators with pure point spectrum in a complex Hilbert space. We find sufficient conditions for such expansions to be unconditionally convergent in spaces with two norms and also estimate the degree of this convergence. Our result essentially generalizes and complements the known theorems of Krein and of Krasnosel'skiĭ and Pustyl'nik. We apply it to normal elliptic pseudodifferential operators on compact boundaryless $C^{\infty }$ -manifolds. We find generic conditions for eigenfunction expansions induced by such operators to converge unconditionally in the Sobolev spaces $W^{\ell }_{p}$ with $p>2$ or in the spaces $C^{\ell }$ (specifically, for the $p$ -th mean or uniform convergence on the manifold). These conditions are sufficient and necessary for the indicated convergence on Sobolev or Hörmander function classes and are given in terms of parameters characterizing these classes. We also find estimates for the degree of the convergence on such function classes. These results are new even for differential operators on the circle and for multiple Fourier series.

A simple model of two-stage choice

This article studies a simple choice model in which two menu-dependent preferences jointly determine behavior. By relaxing multiple assumptions of rational choice theory, our model accounts for many choice anomalies. Nevertheless, we show that the model still possesses empirical content by characterizing it with squeezing conditions directly generalized from standard contraction and expansion conditions. Due to its generality, our model can serve as an umbrella under which three lines of nonstandard choice procedures can be organized: maximization of menu-dependent preference, preference maximization over part of the menu, and the rational shortlisting method. Our model is more than an organizational device because the two preferences can be interpreted as “simple and heuristic” and “complex and deliberate” decision-making methods with multiple selves who are divided into coalitions.

Z Boson Emission by a Neutrino in de Sitter Expanding Universe

The production of Z bosons in emission processes by neutrinos in the expanding de Sitter universe is studied by using perturbative methods. The total probability and transition rate for the spontaneous emission of a Z boson by a neutrino is computed analytically; then, we conduct a graphical analysis in terms of the expansion parameter. Our results prove that this process is possible only for large expansion conditions of the early universe. Finally, the density number of Z bosons is defined, and we obtain a quantitative estimation of this quantity in terms of the density number of neutrinos.

Determinants of integrating women and children-friendly village programs with Anti-drug programs and Stakeholder-Based inclusive village programs

Purpose: Considering the recent phenomenon, there has been an increase in the types and sources of village programs, but program implementation often needs improvement. The cause of failure is thought to be that the ministry designed and implemented the program unilaterally, running partially without stakeholder involvement. Therefore, the objectives of this research are (1) to explain stakeholder involvement and (2) to identify critical factors for the success of village program integration. Research methodology: To the research objectives, the method used is a micro-qualitative method through social mapping as an instrument for community development models. Results: The results obtained are research findings, which consist of (a) the village government's initiation through discussion forums succeeded in equalizing stakeholder perceptions and (b) initiation becomes a way for program integration and an arena capable of encouraging the development of program democratization. Limitations: The research limitation is that the research area only covers one village, so the conclusions obtained are micro. The scope of the substance and area must be expanded to obtain a more comprehensive picture and more in-depth formulation of the findings. Contribution: The research explains that program integration supports improving the socio-economic conditions of village communities, strengthening collaboration, sharing resources, responsibility, innovation, and program expansion.

Insights in the Physicochemical and Mechanical Properties and Characterization Methodology of Perlites

Perlite is a volcanic glass that, under thermal treatment, expands, producing a highly porous and lightweight granular material which finds application in the construction, horticulture, insulation and other industrial sectors. Proper control of the feed properties and the expansion conditions allows the production of purpose-oriented grades, while the primary evaluation of its appropriateness for use in each sector is performed by the proper characterization of relevant physical, thermal or/and mechanical properties. However, due to its extreme fineness, low density, and friability, most of the available characterization methods either fail in testing or provide erroneous results, while for certain properties of interest, a method is still missing. As a consequence, the way towards the evaluation of the material is rife with uncertainties, while a well-defined methodology for the characterization of the critical properties is of practical importance towards the establishment of a pathway for its proper analysis and assessment. This article presents the available methodology for determining the main properties of interest, i.e., the size and density, water repellency/absorption and oil absorption, the microstructural composition, crushing and abrasion resistance and isostatic crushing strength, and also sampling and size reduction processes. The issues raised by the application of existing methods are analyzed and discussed, ending up to a proper methodology for the characterization of each property, based on the long-term experience of the Perlite Institute. The study is supplemented by updated insights on ore genesis, physicochemical properties, mineralogical composition and the expansion mechanism, as background information for the sufficient comprehension of the nature and properties of perlite.

Cardioprotection with levocarnitine intracoronary administration during percutaneous coronary interventions

Currently, the number of percutaneous coronary interventions (PCI) performed in acute and chronic forms of coronary heart disease continues to grow: every year their number reaches at least 5,000,000 worldwide, and more than 200,000 of them in Russia. The main causes of postoperative mortality are perioperative myocardial infarction and acute heart failure due to inadequate protection of the myocardium from ischemia/reperfusion under conditions of balloon expansion and stenting of the affected coronary arteries. The review presents experimental and clinical literature data on the successful use of levocarnitine for cardioprotection in patients with various forms of coronary heart disease and patients with chronic heart failure, both with intravenous administration and as part of a cardioplegic solution during heart surgery. The intracoronary route of administration of levocarnitine solution during PCI in high-risk patients (elderly and senile patients, with multivessel lesions of the coronary bed, difficulties in conducting the intervention) is substantiated. The description of two clinical cases of the use of the technique in elderly patients with acute forms of coronary heart disease with multivessel lesion is given. The postoperative period proceeded without complications with smooth dynamics of biomarkers (troponin I, total creatinephosphokinase, MB-fraction of creatinephosphokinase, lactate dehydrogenase), ischemic ECG shifts were little pronounced. The expected results of the application of the technique are a reduction in intraoperative and postoperative complications of ischemia/ reperfusion and an increase in the effectiveness of the clinical results of PCI in high-risk patients.

New ethics as a reaction to globalization

Topicality. In the modern geopolitical space, the most urgent issue is the national security of individual states in the conditions of various forms of geopolitical expansion. Ukraine is at the epicenter of these extremely difficult conditions. The national security of our country is under threat. Socio-political forecasts and globalization processes point to the reformatting of the consciousness not only of an individual nation, but also of the entire world society. The cultures of the peoples of the entire planet Earth are under the powerful influence of globalization processes. The purpose of the article is to analyze the expected and unexpected changes that we will receive as a result of this large-scale and multifaceted process. especially the emergence of new ethics as a reaction to globalization. Therefore, the main tasks are to determine the possible interpretations of globalization processes in the conditions of geopolitical expansion, the main manipulative components: politics, economy, mass culture, mass media; analysis of consequential phenomena, such as: deformed consciousness of individuals and society as a whole, crisis of self-understanding in the globalized world, risks of loss of identity. As a result of the research, it was found that we associate the emergence of new narratives, norms, and prescriptions with the emergence of new ethics, which will become the main regulator within the limits of civilized globalization. As a conclusion, it can be argued that the most important problem remains the place of man and his relationships in the world of total globalization.

СОЦИАЛЬНО-ЭКОНОМИЧЕСКИЕ ФАКТОРЫ ПРОДВИЖЕНИЯ УСЛУГ ОРГАНИЗАЦИИ

The article provides a theoretical understanding of the features of the formation of a system of strategies for promoting the services of a commercial organization in modern conditions. The need to study and assess the degree of influence of factors on the development of the strategy for promoting the services of the organization is indicated. Socio-economic factors affecting the effectiveness of the promotion of the organization’s services are considered. It was noted that today, in conditions of active digitalization, organizations are increasingly using various digital channels of communication and dissemination of information about the services provided. A synthesis of the methods and factors of service promotion considered in the article, an analysis of the study of factors and conditions for business expansion can increase the efficiency and productivity of a modern organization. Analysis and understanding of the peculiarities of the influence of socio-economic factors on the development of the organization in modern conditions and in the future can be useful in developing its strategies taking into account the target needs of clients.

FEATURES OF THE ORGANIZATION OF ENVIRONMENTAL ACCOUNTING IN THE REPUBLIC OF KAZAKHSTAN

The successful functioning of any business entity in a complex and rapidly changing environment of the economy is determined by its competitiveness. A competitive entity is an organization that has a high value and demand for its products. Therefore, one of the most important conditions for business development and expansion is considered to be the correct accounting of environmental costs, along with the skillful Organization of the methodology for accounting for production costs and costing. This article reveals the need to study environmental accounting in modern conditions aimed at achieving the ultimate goal of any production in order to reduce the impact of environmental damage caused by industrial enterprises. The purpose of the article is to disclose the methodology of "environmental cost accounting", which reflects the relationship between economic costs and environmental protection costs, which allows ensuring the competitiveness and reliability of products. During the execution of the article, various research methods were used, including the dialectical approach, monographic research and graphic methods. The results and conclusions obtained from the study are highlighted by the degree of influence of companies on the effective organization of environmental activities of organizations, in addition to demonstrating the ability of companies to use rational methods of effective management of economic and environmental costs and the formation of cost. In the scientific article, the definition of the formation of the target cost is formulated, the importance and methods of environmental accounting are studied to determine the competitiveness of products and the impact of production on the environment.

Research on the Synchronization Control of Fractional-Order Complex Networks Based on Switching Topology

Abstract: In the contemporary epoch, bolstered by information technology, the quintessence of networks is ubiquitously manifested, with a plethora of network types—ranging from the Internet, vehicular traffic frameworks, electrical distribution systems, cellular communication matrices, to social interconnection webs—being intricately woven into the fabric of societal functionality and quotidian existence. The domain of complex networks has burgeoned into a fervently pursued research vector, magnetizing an eclectic cohort of investigators from disciplines as variegated as mathematics, biosciences, and engineering. Notably, the fractional calculus has eclipsed its integer-order counterpart by offering enhanced precision in the depiction of real-world systems and phenomena. Consequently, the infusion of fractional calculus into the modeling of complex networks, to dissect their dynamic attributes and attendant control paradigms, has crystallized as a research nexus of burgeoning interest, eliciting scholarly discourse at both national and international echelons. [Purpose] This inquiry into the synchronization control of fractional-order complex networks, predicated on switching topology, endeavors to harness said topology as a scaffold for probing the synchronicity inherent within fractional-order complex networks. The objective is to augment the operational efficacy of these networks, broaden their sphere of applicability, and fortify the synchronal linkage amongst fractional-order complex networks and their counterparts. [Method] Predominantly, this exploration is underpinned by a synthesis of bibliographic scrutiny and analytical modeling, employing an extensive compendium of model equations to elucidate the subject matter. The spotlight is cast upon the Caputo fractional-order differential equation, with a focus on assaying the stability traits of its equilibrium junctures and formulating more expansive and pragmatic conditions for stability. In addition, to facilitate the precise estimation of elusive topologies within complex networks, a supplementary network—comprising isolated nodes and a regulatory protocol—is conceptualized. [Results] The findings suggest that the investigation into synchronization control based on switching topology propels the advancement of fractional-order complex networks and has substantial reference value. It serves to substantiate, to a certain degree, the postulations of antecedent theorists and chart a trajectory for ensuing scholarly endeavors in cognate domains, thereby perpetually amplifying the pragmatic utility of fractional-order complex networks.

Reshaping the understanding of the paratethys using paleogeographic reconstructions and geochronology studies

The history of the Paratethys and its inhabiting organisms was profoundly influenced by its connections with the Global Ocean. As the sea?straits linking Paratethys with the ocean expanded, they integrated the Paratethys with the global ocean, causing its water level to mirror the global eustatic fluctuations. These intervals of increased ocean connectivity existed during most part of the Oligocene?Early Miocene, in the Early Badenian?Tarkhanian, and in the Late Badenian?Konkian, when also fostered favourable environments for marine life to flourish in the Paratethys. When the marine connections became unstable and the sea?straits closed (e.g., in the second half of Rupelian?Solenovian time, late Ottnangian?Kozakhurian, middle Badenian?Karaganian and from the onset of the Sarmatian up to the Pliocene), transformed into vast lacustrine waterbodies where water levels and salinities were controlled by the balance between river runoff and precipitation versus evaporation. Excess of water led to the basin expansion and brackish conditions, while deficits caused contraction and fragmentation. This resulted in hypersaline basins and lagoons co?existing with freshwater lakes, creating diverse environments and faunas that hindered effective biostratigraphic correlations. These fluctuations caused the decline of the marine biota, leaving behind only euribiontic organisms capable of enduring drastic shifts in salinity, ion composition, and oxygen levels. Over time, these survivors diversified and gave rise to endemic faunal communities adapted to the brackish environments. The history of the faunistic and phytoplankton composition of the Carpathian part of the basin (Central Paratethys) became noticeably different from the Euxine?Caspian ones from the beginning of the Neogene, which led to different stratigraphic schemes of these parts of the Paratethys. In recent decades, our focus has centred on refining our understanding of the Eastern Paratethys paleogeography. While the periods of high base levels in the Eastern Paratethys are well?documented, the significant base?level drops during isolation phases have often been overlooked. This was primarily because, until recently, there were no effective methods for reconstructing the paleogeography and geo ? chronology of Paratethys during these episodes of base?level drops. However, with the advancements in seismic stratigraphy and the utilization of integrated magneto?bio?stratigraphy, we now possess the means to explore the scale and spatial distribution of paleogeographic changes, particularly during the substantial basin reductions in partial desiccation periods. Large regressions (during second part of the Rupelian, terminal Tarkhanian, and Sarmatian in the Eastern Paratethys and during early Pliocene in the Caspian basin) erode parts of sedimentary layers, forming surfaces of inconsistency between geological bodies accumulated before and after the sea?level drop, which can be traced on seismic profiles as erosion boundaries. By deciphering such surfaces and the depth of river incisions flowing into the basin, it is now possible to reconstruct the relief picture resulting from these erosions and quantify the depth of baselevel drops in the Paratethys during the largest regressions.

Navigating the Adipocyte Precursor Niche: Cell-Cell Interactions, Regulatory Mechanisms and Implications for Adipose Tissue Homeostasis.

Support for stem cell self-renewal and differentiation hinges upon the intricate microenvironment termed the stem cell 'niche'. Within the adipose tissue stem cell niche, diverse cell types, such as endothelial cells, immune cells, mural cells, and adipocytes, intricately regulate the function of adipocyte precursors. These interactions, whether direct or indirect, play a pivotal role in governing the balance between self-renewal and differentiation of adipocyte precursors into adipocytes. The mechanisms orchestrating the maintenance and coordination of this niche are still in the early stages of comprehension, despite their crucial role in regulating adipose tissue homeostasis. The complexity of understanding adipocyte precursor renewal and differentiation is amplified due to the challenges posed by the absence of suitable surface receptors for identification, limitations in creating optimal ex vivo culture conditions for expansion and constraints in conducting in vivo studies. This review delves into the current landscape of knowledge surrounding adipocyte precursors within the adipose stem cell niche. We will review the identification of adipocyte precursors, the cell-cell interactions they engage in, the factors influencing their renewal and commitment toward adipocytes and the transformations they undergo during instances of obesity.

Insights of the peroxychloroformyl radical ClC(O)OO via microwave spectrum.

The exploration of Venus has received much attention in the past and will keep growing due to the starting of the NASA DAVINCI project. To explain the extremely low O2 : CO ratio observed in Venus' atmosphere, a chlorine-initiated CO oxidation catalytic cycle has been proposed. However, relevant studies on the key intermediates, such as the peroxychloroformyl radical (ClC(O)OO), are rare. In this study, the ClC(O)OO radical was observed using Fourier-Transform Microwave (FTMW) spectroscopy under the supersonic expansion condition. Two conformers, trans-ClC(O)OO and cis-ClC(O)OO, and their chlorine isotopologues were detected. The molecular constants including the fine and hyperfine constants, were determined. Based on the experimental and the ab initio calculations, the unpaired electron is mostly located at the terminal oxygen atom, supported by the small magnetic hyperfine constants of chlorine. In addition, the angles between the Cl-C bond and the a-axis of the trans-35ClC(O)OO and trans-37ClC(O)OO are similar, but these angles are different for cis-ClC(O)OO, making the quadrupole coupling tensors in the inertial axes disagree with the ratio of the quadrupole moments of 35Cl and 37Cl. Finally, we concluded that the ClC(O)OO radicals should behave similarly to other peroxyl radicals, as assumed in the current photochemical model of Venus.

Homochiral vs. heterochiral preference in chiral self-recognition of cyclic diols.

The structure and clustering propensity of a chiral derivative of cis-1,2-cyclohexanediol, namely, 1-phenyl-cis-1,2-cyclohexanediol (cis-PCD), has been studied under supersonic expansion conditions by combining laser spectroscopy with quantum chemistry calculations. The presence of the phenyl substituent induces conformational locking relative to cis-1,2-cyclohexanediol (cis-CD), and only one conformer of the bare molecule is observed by both Raman and IR-UV double resonance spectroscopy. The homochiral preference inferred for the dimer formation at low enough temperature is in line with the formation of a conglomerate in the solid state. The change in clustering propensity in cis-PCD relative to trans-1,2-cyclohexanediol (trans-CD), which shows heterochiral preference, is explained by the presence of the phenyl substituent rather than the effect of cis-trans isomerism. Indeed the transiently chiral cis-CD also forms preferentially heterodimers, whose structure is very close to that of the corresponding trans-CD dimer.

High-speed railway ripples on the greenness: Insight from urban green vegetation cover

Less attention has been paid to the potential ecological pressures brought about by high-speed rail (HSR) while promoting urban prosperity. Based on Normalized Difference Vegetation Index and multiple socio-economic and ecological data, this study examines the local effect and spatial spillover effect of HSR operation on green vegetation coverage (GVC) in Chinese cities from 2003 to 2014, using a difference-in-differences design and spatial econometrics. Main findings are: 1) HSR operation has reduced GVC of local cities by about 6.0%. Because HSR-triggered economic agglomeration has intensified the land development and increased the pollution emissions (represented by SO2 and dust), leading to the degradation of local green vegetation. 2) The local effects of HSR on urban GVC are heterogeneous. HSR operation seriously cuts down the GVC for development-saturated cities, slightly undercuts the GVC for cities with conventional railways, but positively boosts the GVC for ecotourism cities. 3) HSR operation increases the GVC of adjacent cities (cities contiguous to HSR-located cities) by about 3.8%. Because the economic siphoning effect of HSR on adjacent cities reduces their land needs and pollution emissions, thus providing conditions for the vegetation expansion. Note that the HSR’s impact on adjacent cities’ GVC is influenced by the economic disparity between HSR cities and their neighbors. This study re-identifies the role of the transportation infrastructure in reshaping ecological patterns, providing insights into regional sustainable development.

Abstract A036: Development of a stirred-tank bioreactor scale-up approach to allogeneic natural killer cell culture

Abstract Natural killer (NK) cells are innate lymphocytes that play a critical role in the immune response, as they recognize and eliminate virus-infected, cancerous, and other abnormal cells in an antigen-independent manner. This unique method of recognition makes NK cells an attractive target for utilization as an immunotherapy, as studies have demonstrated they reduce both the induction of graft-versus-host disease and the risk of cytokine release syndrome compared to T-cell based therapies. Additionally, these advantages over canonical T-cell therapies make NK therapies attractive candidates for allogeneic, as opposed to autologous, products. Given their cost-effective scale-up potential, developing a stirred-tank bioreactor (STR)-based NK cell expansion platform to achieve clinical-level expansion is of significant interest, as it would reduce the cost of producing such therapies and improve patient accessibility. To test ideal culture conditions for STR expansion, NK cells were first isolated from healthy donor Leukopaks prior to cryopreservation. Frozen, isolated NK cells were then thawed, co-cultured with varying ratios of irradiated K562 cells and supplemental cytokines from Cytiva in a 6-well G-Rex or DASbox® STR. Finally, cells were harvested for downstream analytics, including identity and functional characterization by flow cytometry and potency assays. A fold expansion of greater than 100 was achieved once the ratio of irradiated K562 to NK cells was optimized. One of the primary challenges encountered was donor-to-donor variation, not only with respect to proliferative capacity, but NK functional phenotype and activity as well. Therefore, a small-scale donor screen was conducted and donors with similar proliferative and functional capabilities were selected for large-scale expansion. Future work will focus on optimizing NK cell transduction to enable genetic customization and scaling-up to a clinically-relevant STR. The successful small-scale NK expansion in an STR demonstrated here lays the groundwork for an expansion platform that enables the manufacturing of various NK-based therapeutics at clinical scale. Citation Format: Mathura Yogalingam, Rebecca Jagroop, Alireza Khalajzadeh, Thergiory Irrazabal, Melissa Perea, Pooja Pushparaj, Roxana Roshankhah, Paul Bowles, Michael Le. Development of a stirred-tank bioreactor scale-up approach to allogeneic natural killer cell culture [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Tumor Immunology and Immunotherapy; 2023 Oct 1-4; Toronto, Ontario, Canada. Philadelphia (PA): AACR; Cancer Immunol Res 2023;11(12 Suppl):Abstract nr A036.

Groundwater management in the face of climate change: enhancing groundwater storage in the alluvium aquifer of Wadi Araba, Jordan, through GIS-based managed aquifer recharge and groundwater MODFLOW

Abstract Groundwater is critical in countries such as Jordan, yet demand exceeds availability due to population expansion and arid conditions. The goal of this research is to address water scarcity and adapt to reduced rainfall by investigating the soil aquifer and evaluating the efficiency of managed aquifer recharge (MAR). The Wadi Araba Basin's alluvium aquifer is particularly important and contains a groundwater divide, with water flowing towards the Red Sea to the south and the Dead Sea to the north, as determined by rigorous modelling and scenario analysis. Precipitation infiltration is an important consideration in groundwater budget modelling. This study employs 12 monitoring wells to establish an acceptable relationship between estimated and observed water levels. Furthermore, the study creates a MAR suitability map, which evaluates eight potential MAR locations in the Wadi Araba region. According to forecasted scenarios, implementing MAR in conjunction with increased precipitation recharge has the potential to ameliorate the consequences of decreased rainfall in the model region. The plan aims to raise the water table in three areas by 1.96–3.12%, providing realistic solutions to enhance water availability and adapt to climate change.