Control Condition Research Articles

Effects of 12 weeks of resistance exercise on 24-hour subjective appetite measured under highly controlled conditions inside a metabolic chamber in older men

Effects of 12 weeks of resistance exercise on 24-hour subjective appetite measured under highly controlled conditions inside a metabolic chamber in older men

The application of heating film to hands reduces the decline in manual dexterity performance associated with cold exposure.

Exposure to cold temperatures decreases finger temperature (Tfing) and dexterity. Decreased manual function and dexterity can be serious safety risks, especially in tasks that require fine motor movements that must be performed outdoors. The aim of this study was to determine whether hand heating with a minimal power requirement (14.8W) results in a smaller reduction in Tfing and manual dexterity performance during mild cold exposure compared to a non-heated control condition. In a randomized crossover design, twenty-two healthy participants were exposed to a moderately cold environment (5 ºC) for 90min. One condition had no intervention (CON), while the other had the palmar and dorsal hands heated (HEAT) by using electric heating films. Tfing and cutaneous vascular conductance (CVC) were continuously monitored using laser Doppler flowmetry. Manual dexterity performance and cognitive function were assessed by the Grooved Pegboard Test (GPT) and Stroop Color-Word (SCW) test, respectively, during the baseline period and every 30min during the cold exposure. After the cold exposure, Tfing was higher in HEAT relative to CON (CON 9.8 vs. HEAT 13.7ºC, p < 0.0001). GPT placing time, as an index of dexterity performance, was also shorter in HEAT by 14.5% (CON 69.10 ± 13.08 vs. HEAT 59.06 ± 7.99s, p < 0.0001). There was no difference in CVC between the two conditions during the cold exposure (p > 0.05 for all). Cognitive function was similar between two conditions (p > 0.05 for all). The proposed hand heating method offers a practical means of heating fingers to maintain dexterity throughout prolonged cold exposure.

Influence of Motor Imagery Modality on First-Serve Performance in Tennis Players.

Motor imagery (MI) is frequently used in tennis players. This pilot study aimed to assess whether the MI modality and preference of skilled tennis players could influence their service performance when using MI before serving first balls. Twenty expert players (Mage = 18.6years) completed the movement imagery questionnaire (third version) to assess their MI modality scores (internal visual, external visual, and kinesthetic) and their MI preference. Participants completed 4 experimental counterbalanced sessions spread over 4weeks, each including the completion of 20 first-serve balls in match condition. The sessions included a control condition (i.e.,only physical practice trials) and three MI conditions during which the players had to mentally imagine themselves performing a serve according to one of the imagery modalities, either internal visual, external visual, or kinesthetic, before serving. The percentage of success, the speed of the service balls (measured by a tablet with SWING VISION and a radar gun), and an efficiency score were recorded and then evaluated by experts and served as performance indicators and dependent variables. The results of this study showed that players benefited from MI before serving and that almost a third of the participants achieved a higher percentage of success and efficiency scores when using their preferred MI modality. These results lead us, in an applied way, to suggest to skilled tennis players to determine their MI preference and to have recourse to the mental simulation of a successful serve before serving the first balls in match condition.

Research on the response of underwater explosion side structure based on RKPM method

Abstract Based on smoothed particle hydrodynamics and reproducing kernel particle method, this paper establishes a fluid-structure interaction three-dimensional calculation model for the dynamic response of complex structures to underwater explosion shockwave, verifies the effectiveness, accuracy and progressiveness of SPH-RKPM coupling calculation, and establishes a control condition with detonation distance as a variable to determine the dynamic response characteristics of ship side under shockwave loads generated by underwater explosion at different detonation distances.

Contextual modulation of human associative learning following novelty-facilitated extinction, counterconditioning, and conventional extinction.

The expression of an association between a conditioned stimulus (CS) and an unconditioned stimulus (US) can be attenuated by presenting the CS by itself (i.e., extinction, Ext). Though effective, Ext is susceptible to recovery effects such as renewal, spontaneous recovery, and reinstatement. Dunsmoor et al. (2015, 2019) have proposed that pairing the CS with a neutral outcome (novelty-facilitated Ext [NFE]) could offer better protection against recovery effects than Ext. Though NFE has been compared to Ext, it has rarely been compared to counterconditioning (CC), a similar procedure except that the CS is paired with a US having a valence opposite to the US used in initial training. We report two aversive conditioning experiments using the rapid-trial streaming procedure with human participants that compare the efficacies and susceptibilities to ABA renewal of Ext, CC, and NFE. Associative learning was assessed through expectancy learning and evaluative conditioning. CC and NFE equally decreased anticipation of the US in the presence of the CS (i.e., expectancy learning). Depending on how the CS-US association was probed, they were either as or more effective at doing so than Ext. All three interference treatments were equally susceptible to context manipulations. Only CC clearly altered the valence of the CS (i.e., evaluative conditioning). Valence ratings after Ext, CC, and NFE, as well as a no-interference control condition, were all equally susceptible to context effects. Overall, the present study does not support the assertion that NFE is consistently more resistant to recovery effects than Ext. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Chronic effects of a high protein diet on body mass, energy intake, and 24-hour subjective appetite measured under highly controlled conditions inside a metabolic chamber in older men

Chronic effects of a high protein diet on body mass, energy intake, and 24-hour subjective appetite measured under highly controlled conditions inside a metabolic chamber in older men

A randomized controlled trial testing the efficacy of the eCHECKUP TO GO on drinking games participation and behavior among high school seniors

BackgroundAmong high school students, seniors report the highest levels of hazardous drinking behavior, including playing drinking games. Technology-based interventions are a promising approach for reducing hazardous drinking behavior among this age group. Objectives: This randomized controlled trial investigated the efficacy of the eCHECKUP TO GO, an online personalized feedback intervention, on reducing the frequency of playing drinking games, the number of drinks consumed while playing drinking games, and the number of drinks consumed on occasions when drinking games were played among high school seniors (ClinicalTrials.gov Identifier NCT03613818). Method: Participants were recruited from two high schools. Class periods were randomized to the intervention condition or an assessment-only control condition. Participants completed online surveys at baseline, 30-day, and 6-month assessments. The subsample in this study (N = 109) consisted of high-risk drinkers (i.e., students reporting binge drinking in the past two weeks at baseline). Results: We did not find any significant differences in frequency of playing drinking games between the intervention and control conditions. For number of drinks consumed, students in the intervention condition reported a significant reduction in the number of drinks consumed while playing drinking games (p < 0.01) and total number of drinks consumed on drinking game occasions (p < 0.04) at the 30-day follow-up relative to students in the control condition. Reductions within the intervention group were sustained at the 6-month follow-up. Conclusions: Results support the efficacy of the eCHECKUP TO GO for decreasing hazardous alcohol use among high school seniors.

Time course and neural locus of the Flashed Face Distortion Effect

Viewing a rapid sequence of face images shown in the periphery can lead to large caricature-like distortions in the perceived images, a phenomenon known as the Flashed Face Distortion Effect (FFDE). The mechanisms underlying FFDE are poorly understood. Here we examined the timing and sites of the adaptation processes giving rise to the FFDE. To investigate the effects of presentation rate, we maintained consistent trial lengths while assessing how variations in the temporal frequencies of face presentation influenced the magnitude of face distortion and the averaging of facial expressions. Over a wide range of temporal frequencies (1.2–60 Hz) tested, we observed a decrease in FFDE strength as the presentation rate increased. To probe the neural sites of FFDE, we varied whether successive faces were presented to the same or different eyes using a dichoptic display. Distortion effects were comparable for monocular, binocular, and interocular conditions, yet much larger than a control condition where faces were presented with a temporal interval between successive images, suggesting a cortical locus for FFDE.

Automated patient selection and care coaches to increase advance care planning for cancer patients.

Advance care planning/serious illness conversations can help clinicians understand patients' values and preferences. There are limited data on how to increase these conversations, and their effect on care patterns. We hypothesized that using a machine learning survival model to select patients for serious illness conversations, along with trained care coaches to conduct the conversations, would increase uptake in cancer patients at high risk of short-term mortality. We conducted a cluster-randomized stepped wedge study on the physician level. Oncologists entered the intervention condition in a random order over six months. Adult patients with metastatic cancer were included. Patients with <2 year computer-predicted survival and no prognosis documentation were classified as high-priority for serious illness conversations. In the intervention condition, providers received automated weekly emails highlighting high-priority patients and were asked to document prognosis for them. Care coaches reached out to these patients to conduct the remainder of the conversation. The primary endpoint was proportion of visits with prognosis documentation within 14 days. 6,372 visits in 1,825 patients were included in the primary analysis. The proportion of visits with prognosis documentation within 14 days was higher in the intervention condition than control condition: 2.9% vs 1.1% (adjusted odds ratio 4.3, p < .0001). The proportion of visits with advance care planning documentation was also higher in the intervention condition: 7.7% vs 1.8% (adjusted odds ratio 14.2, p < .0001). In high-priority visits, advance care planning documentation rate in intervention/control visits was 24.2% vs 4.0%. The intervention increased documented conversations, with contributions by both providers and care coaches.

Hydrogel-Integrated Heart-on-a-Chip Platform for Assessment of Myocardial Ischemia Markers.

Organ-on-a-chip platform scans offer a controllable environment and a physiological similarity to mimic human pathophysiology. In this study, a single-channel PDMS microchip was fabricated, characterized, and optimized to obtain a heart-on-a-chip platform, which is integrated with a hydrogel scaffold suitable for cardiomyocyte growth inside its channel. Single-channel chips with a size of 20 × 12 mm and a channel height ranging from 60 to 100 μm were produced using photolithography and soft lithography techniques. A gelatin-embedded alginate network-based hydrogel was further augmented with 3% (v/v) collagen type I. Pore sizes were in the range of 74-153 μm for H9C2 implantation and biomimicry. The hydrogels are characterized both on PDMS surfaces and in capillaries. The primary feature distinguishing this study from previous microchip studies is that it mimics the cell microenvironment much better using different hydrogel formulations instead of creating a 2D cell culture by passing fluids, such as fibronectin, for cell adhesion. Instead of using complex microchip designs, the chip system we created intends to provide a physiologically relevant copy by using a 3D cell culture to its advantage and a simple, single-channel architecture. The microchip study was combined with cardiomyocytes to create the heart-on-a-chip system and tested under normoxic and hypoxic conditions to create a myocardial ischemia model inside this channel. As a result, this heart-on-a-chip platform was shown to be utilized for the detection of several small-size biomarkers such as adenosine, ADP, lactic acid, l-isoleucine, l-glutamic acid, and oxidized glutathione via LC-MS/MS from control conditions and a myocardial ischemia model. Cell-embedded and hydrogel matrix-supported versions of this heart-on-a-chip system were successfully prepared and shown to provide powerful outputs with myocardial ischemia markers. In light of this research, these outputs aim to develop simple and biologically effective organ-on-a-chip systems for future research.

중국 대학생의 기대된 평가와 목표지향성이 창의성에 미치는 영향

The purpose of this study was to investigate the effects of expected evaluation and goal orientation on creative performance. In Study 1, the three-factor goal orientation latent profile of 1,333 Chinese college students was analyzed. In Study 2, the effects of expected evaluation and goal orientation latent profile on creativity were investigated for 399 subjects. As a result, Study 1 identified four latent profiles: ‘1-mastery-approach goal type’, ‘2-medium-low type’, ‘3-high achievement type’, and ‘4-medium-high type’. In Study 2, the main effects of expected evaluation (no evaluation, informational evaluation, and controlling evaluation) on fluency, flexibility, and originality were statistically significant, and the main effect of goal orientation latent profile on originality was significant. The interaction effect between expected evaluation and goal orientation latent profile was significant only on originality. That is, ‘3-High Achievement Orientation’ showed the highest originality in the informational evaluation condition, while ‘1-Mastery Approach Goal Type’ and ‘4-Medium-High Type’ showed the highest originality in both the informational evaluation and no-evaluation conditions. All three latent groups showed the lowest originality in the control condition. ‘2-Medium-Low Type’ did not interact with the expected evaluation type. The following is a discussion of the research results, their implications, and their limitations and suggestions.

Feasibility and acceptability of a telehealth, individually-tailored healthy lifestyle intervention for adolescent and young adult pediatric cancer survivors.

Poor nutrition and physical activity pose negative health risks for adolescent and young adult pediatric cancer survivors (AYACS). Our pilot randomized controlled trial (RCT) evaluated feasibility and acceptability of a telehealth intervention (ENHANCE) supporting AYACS' nutrition and physical activity. We randomized 58 AYACS and their adult care partner, when applicable, to the ENHANCE or control condition. Inclusion required an age of 15-25 years, previous chemotherapy or radiation therapy, and no eating disorder history. We utilized a 2:1 (ENHANCE [n = 35]: control [n = 23]) double-blind block randomization protocol in blocks of 6. ENHANCE comprised 12 (60-min) sessions that included education, skill-building, and values-based healthy lifestyle decision-making. The first five sessions included motivational interviewing and the final seven sessions included problem-solving training/goal setting. We evaluated feasibility based on eligibility, enrollment, attrition, and study completion percentages. We assessed fidelity using the Session Content-Fidelity Rating Tool and the Motivational Interviewing Treatment Integrity Coding form. We assessed acceptability using the Abbreviated Acceptability Rating Profile, the Revised Helping Alliance Questionnaire, and participants' exit interviews. ENHANCE was feasible and highly acceptable; all but one participant who started the intervention completed all 12 sessions and all participants reported enjoying the intervention via objective assessment and during exit interviews. Interventionists delivered ENHANCE with high fidelity. ENHANCE was highly acceptable among AYACS and care partners. The telehealth study design was feasible, suggesting benefits and feasibility of a future, larger, multisite RCT. Promising findings support our methodological decisions as important considerations to inform a larger RCT.

Sustained co-release of ciprofloxacin and dexamethasone in rabbit maxillary sinus using polyvinyl alcohol-based hydrogel microparticle

Topical delivery to paranasal sinuses through sustained-release stents is one of the new horizons in treating chronic rhinosinusitis (CRS). This study aims to introduce and evaluate sustained co-release of encapsulated ciprofloxacin (CIP) and dexamethasone (DEX) in polyvinyl alcohol-based carriers within the maxillary sinus of rabbit animals. DEX and CIP were loaded in a tyramine-substituted polyvinyl alcohol microparticle (PVATyr MP). The mechanical stability, degradability, and sustained-release patterns of both drugs as well as cellular cytocompatibility were assessed in vitro. The PVATyr MPs were then injected into the maxillary sinus of rabbits and they were monitored weekly for 21 days. Nasal endoscopy, MRI imaging, and tissue microscopy were used to follow the changes and compared them with the control condition. Also, the concentrations of drugs were evaluated in the maxillary sinus and blood samples over the study period. Produced PVA-based MPs possessed a relatively narrow particle size distribution (CV 7.7%) with proper physical stability until 30 days of incubation. The uniform-sized PVATyr MPs and their surrounding hydrogel showed sustained-release profiles for DEX and CIP for up to 32 days in vitro. The injected drugs-loaded hydrogel showed complete clearance from the maxillary sinus of rabbits within 28 days. The concentrations of DEX and CIP in mucosal remained within the therapeutic window when measured on days 7, 14, and 21, which were well above the plasma concentrations without any pathological changes in endoscopy, MRI imaging, and histological examinations. DEX/CIP loaded PVATyr MPs provided an effective, controlled, and safe sustained-drug delivery in both in vitro and in vivo analyses at therapeutic concentrations with minimal systemic absorption, suggesting a promising treatment approach for CRS.Graphical

Effect of Pre-Harvest Intermittent UV-B Exposure on Growth and Secondary Metabolites in Achyranthes japonica Nakai Microgreens in a Vertical Farm

Achyranthes japonica Nakai (AJN) is a medicinal plant known to be beneficial for the joints. Since it takes at least two years from sowing to harvesting in an open field, new AJN cultivation strategies are needed to shorten the production period and improve quality. In this study, high-quality AJN is produced as microgreens in a vertical farm using a commercial ginseng soil mix (Myeongpum-Insamsangto, Shinsung Mineral Co., Ltd., Goesan, Republic of Korea) and controlled environmental conditions. The cultivation conditions included a temperature of 23 ± 2 °C, relative humidity of 50 ± 10%, and a photosynthetic photon flux density of 170 ± 15 µmol·m−2·s−1. Pre-harvest intermittent UV-B exposure, with an intensity of 1.0 ± 0.3 W/m−2, was applied for one day before harvest to evaluate its effects, using controlled environmental conditions in the vertical farm. Ultraviolet-B (UV-B) irradiation increases secondary metabolite levels in plants; however, the effect of UV-B on 20-hydroxyecdysone (20E), an indicator of AJN, is unclear. Therefore, we aimed to investigate whether UV-B treatment of AJN microgreens affected growth and secondary metabolites. The treatment group was set to 12 h of continuous UV-B treatment during the day, two 6 h UV-B treatments, and four 3 h UV-B treatments to confirm the effectiveness of regular and intermittent treatment and recovery. Short-term UV-B treatment before harvesting increased phenols, flavonoids, antioxidant capacity, and 20E levels without affecting AJN biomass. The intermittent 6 h UV-B irradiation with a 6 h recovery time stimulated 20E content by approximately 1.4 times compared to the control. These study findings indicate that short-term UV-B treatment before harvesting, an appropriate recovery time, and intermittent UV-B exposure are more effective at increasing 20E content than continuous treatment. This approach provides a promising strategy for improving the nutritional and health benefits of AJN microgreens in vertical farming systems.

Elevated pCO2 may increase the edible safety risk of clams exposed to toxic Alexandrium spp.

Toxic harmful algal blooms (HABs) have received increasing attention owing to their threat to the health of aquatic life and seafood consumers. This study evaluated the impacts of elevated atmospheric partial pressure of CO2 (pCO2) on the production of paralytic shellfish toxins (PSTs) in different Alexandrium spp. strains, together with its further effects on the bioaccumulation/elimination dynamics of PSTs in bivalves contaminated with PSTs from toxic dinoflagellates. Our results showed that elevated pCO2 stimulated the growth of the two Alexandrium spp. (A. catenella and A. pacificum) isolated from the northern and southern coastal areas of China, respectively, and affected PST production including content and toxicity of the two strains differently. Further PSTs bioaccumulation/elimination in PSTs-contaminated Manila clam, Ruditapes philippinarum under high pCO2 also occurred. It is worth noting the biotransformation of neosaxitoxin (NEO) with high toxicity through trophic transfer with effect of elevated pCO2. When in microalgae cultured under the control (410 ppm) and elevated pCO2 conditions (495 and 850 ppm), the proportion of NEO in the PST content produced by A. catenella was reduced from 11.1 to 6.4 and 2.6 %, while the proportion of NEO in A. pacificum was increased from 3.1 to 3.6 and 4.7 %, respectively. NEO accounted for >50 % of total PST contents in clams, which were biotransformed via transfer from dinoflagellates and higher pCO2 enhanced this biotransformation leading to increased NEO accumulation. The negatively affected elimination of PSTs, especially NEO, in clams fed with A. catenella or A. pacificum, indicates that the detoxification of PSTs-contaminated clams may be more difficult under elevated pCO2. This study provides reference for developing models to assess the safety of bivalves under the co-stress of environmental change and toxic HABs, suggesting that ocean acidification may lead to the higher safety risk of Manila clams exposed to toxic HAB dinoflagellates.

Evaluation of the Secondary Metabolites and Bioactivity of South African Bulbine Natalensis Under Simultaneous Elevated Carbon Dioxide and Temperatures

The secondary metabolism in medicinal plants are responsible for their protection against environmental factors and the health-promoting benefits they provide to users. The purpose of this study was to assess the responses of the secondary metabolic system of B. natalensis and its influence on the antioxidant and antibacterial activities. The phytochemical analyses, antioxidant and antibacterial assays were conducted from using harvested leaves, underground stems and roots from whole plants exposed to simultaneous elevated CO2 and temperatures over eight days in a heatwave simulation. Most of the phytochemical groups were present in the leaves, however majority of the tested compounds had consistent presence throughout the plants in all treatments. The underground stems possessed a greater total phenolic, tannin and proanthocyanidin contents than the leaves and roots under elevated CO2 and temperatures. The leaves had a comparatively better antioxidant activity from the treatment than control. Overall, there was consistency in the antibacterial activity in both control and experimental conditions. The tannins and phenolics had greater concentrations throughout the plants under elevated conditions overall, which could indicate their leading role in plant defence and the improvement and maintenance of the medicinal activity of B. natalensis. The responses of the plant parts of B. natalensis under elevated CO2 and temperatures provides newfound insights on the physiological roles plant organs play on the overall productivity and medicinal activity of the species.

More or Less Likeable: The Role of Behavior-Independent Events in the Disposition Formation Process

ABSTRACT Affective disposition theory (ADT) and its extensions posit that character liking/disliking is based on viewer perceptions of character behaviors or the activation of character schemas that imply moral/immoral behaviors. This paper systematically examines whether events that happen to a character independent of their behavior also contribute to the development of character liking. Based on the modified affective disposition model (MADM), two studies were conducted where participants read a story introduction that manipulated whether a positive or negative event (described as egoistic need satisfaction/thwarting by the MADM) had befallen a character. In Study 1, the character was liked more when a negative economic event had befallen them and liked less when a positive economic event had befallen them as compared to a control condition. In Study 2, these effects were replicated. However, in Study 2 we also examined whether health-related events would elicit similar effects. Participants liked the character more regardless of whether a positive or negative health event befell her. These findings suggest unique contextual effects associated with which specific egoistic intuitions are satisfied or thwarted. As such they provide novel directions for research designed to test and specify the predictions of the MADM.

Protocol of a randomized controlled trial into guided internet-delivered cognitive behavioral therapy for insomnia in autistic adults (i-Sleep Autism)

BackgroundSleep problems, especially insomnia, are prevalent among autistic adults, affecting about 60 %, and significantly impact their quality of life. Internet-based cognitive behavioral therapy for insomnia (iCBT-I) could provide accessible and scalable treatment. Given the unique sensory- and information processing, and social challenges at play in autism, a tailored treatment approach may be essential to tackle sleep problems. Yet, interventions developed and tested specifically for autistic adults were scarce. Addressing this gap is crucial to meet the urgent need for effective insomnia treatments in this population. MethodsWith this two-arm, parallel, superiority randomized controlled trial, we will assess the effectiveness of a guided iCBT-I intervention for adults (N = 160) with autism and insomnia (i-Sleep Autism). In co-creation, i-Sleep Autism has been adjusted from an existing intervention (i-Sleep). Inclusion criteria are: age ≥ 18, an ASD diagnosis, and at least sub-threshold insomnia (Insomnia Severity Index ≥10). Participants are randomly assigned to either i-Sleep Autism or an information only waitlist control condition (online psychoeducation and sleep hygiene). After 6 weeks, the control group receives the intervention. Insomnia severity is the primary outcome. Secondary outcomes include pre-sleep arousal, general mental health, depression, anxiety, daily functioning, and quality of life. Assessments will occur at baseline, mid-intervention (3 weeks), post-intervention (6 weeks), and at 6-month follow-up (the intervention group). Linear mixed-effect regression models are employed to evaluate the effectiveness of i-Sleep Autism, alongside exploration of potential moderators and mediators. ConclusionThis trial can reveal whether autistic adults with insomnia benefit from a guided e-health intervention.Trial registration: NL-OMON56692.

Serial engagement of distinct motor learning mechanisms to alter walking after stroke

This study asked if combining different motor learning mechanisms—adaptation and reinforcement—could produce immediate improvements in over ground walking after stroke. Fifteen adults with stroke engaged in three conditions: (1) reinforcement following adaptation, (2) reinforcement alone, and (3) adaptation alone. Adaptation involved split-belt treadmill walking to produce after-effects that reduce step asymmetry. Reinforcement involved the use of real-time auditory feedback about step length asymmetry. Auditory feedback was binary, signaling whether steps were asymmetric or equal, but not whether to shorten or lengthen either step. Change in step length asymmetry was the outcome assessed during over ground walking. Reinforcement following adaptation led to reductions in step length asymmetry that persisted into an immediate retention period. Importantly, it led to the desired pattern of lengthening the shorter step in a majority of participants. Reinforcement alone led to no significant change in step length asymmetry, and sometimes produced a non-optimal pattern of shortening the longer step. Our control condition of adaptation alone led to more transient reductions in step length asymmetry. These findings reveal the potential for utilizing serial delivery of adaptation and reinforcement to influence a complex movement in the real-world context of over ground walking, in people with stroke.

Effect of Heating and Massaging of Meibomian Glands on Their Imaging

Background and Objectives: Infrared light is used to image the Meibomian glands through their thermal profile. This study aimed to investigate the effects of a combination of heating and an eyelid massage on Meibomian gland visibility and tear film parameters. Materials and Methods: Twenty-four participants (26 ± 6.9 years) were enrolled in this prospective study, which involved imaging the Meibomian glands of both the lower and upper eyelid and assessing the non-invasive breakup time (NIBUT), tear meniscus height (TMH), and blink rate (using the CA-800, Topcon) at baseline after five minutes of eyelid warming followed by a five-minute eyelid massage. The second session, which was randomised in sequence, repeated the same measurements but without the inclusion of any eyelid warming or massage as the control condition. Results: While there was no change in lower lid Meibomian gland appearance as a result of eyelid heating, eyelid massage, or multiple lid eversion (median 2.0, range 0.0 to 4.0; p = 0.782), there was a change in upper lid appearance 5 min after heating and lid massage (p = 0.025), but again, multiple lid eversion had no effect (p &gt; 0.05). The NIBUT decreased on second lid eversion (p = 0.049), although this was not evident on the third lid eversion (p = 0.090). The effect on NIBUT was also apparent with heating (p = 0.034 immediately after) but was sustained with 5 min of eyelid massage (p = 0.031). The TMH increased with heating (p &lt; 0.001), and this effect was sustained with 5 min of eyelid massage (p = 0.011), but there was no lid eversion effect (p &gt; 0.05). The blink rate was unaffected by heating, eyelid massage, or multiple eversions of the eyelids (median 24 blinks/min, range 8 to 59 blinks/min; p = 0.61). Conclusions: Eyelid warming can increase the visibility of the Meibomian glands, although this effect was only observed with upper lid imaging and the effect dissipated after 5 min of eyelid massage. Warming and massage also disrupt the tear film, as does multiple lid eversion, emphasising the need to use the least invasive tear film assessment techniques first.