Soil Organic Carbon Content Research Articles

Grazing exclusion promotes soil organic carbon accumulation in Tibetan grasslands with lower temperatures

BackgroundGrazing exclusion is a practical approach to restore vegetation in degraded grasslands and enhance soil organic carbon (SOC) sequestration. However, the dynamics and drivers of SOC in grasslands after grazing exclusion have not been well documented, especially in ecosystems with cold climates.MethodsHere, we established 14 paired treatments (grazing exclusion vs. free-grazing) along a 600-km transect in the northeastern zone of the Qinghai-Tibet Plateau. After six years, we analyzed vegetation biomass dynamics and measured the soil physicochemical properties and organic C concentration across three depths (0–10, 10–20, and 20–30 cm).ResultsGrazing exclusion significantly increased above- and belowground biomass (139.85% and 43.30%, respectively), pH (1.38%), total phosphorus (3.29%), nitrate nitrogen (18.03%), and ammonium nitrogen (17.81%), but significantly decreased soil bulk density (2.43%) and clay content (10.49%), particularly in 0–30 cm. Specifically, SOC concentrations positively responded to grazing exclusion (0–10 cm) in 9 of the 14 sites evaluated. The effects of grazing exclusion on SOC concentrations were significantly higher in areas with a mean annual temperature (MAT) below 0 °C compared to those in sites with a high MAT (> 0 °C). The SOC concentrations significantly correlated with the mean annual precipitation (MAP) in both treatments, but these correlations diminished with increasing soil depth. Ridge regression analysis showed that soil chemical properties (e.g., total nitrogen and phosphorus) positively influenced SOC accumulation, while MAT negatively influenced it after grazing exclusion. Path analysis further revealed that MAT indirectly regulated SOC dynamics via soil chemical properties.ConclusionsOur study highlights that grazing exclusion results in an asynchronous SOC and plant biomass accumulation and may be more beneficial for SOC sequestration in Qinghai-Tibet Plateau grasslands with lower temperatures. Also, humid climates promote SOC concentration in alpine grasslands. These results could help develop management practices and policies that promote sustainable grassland management.

An integrated dataset of ground hydrothermal regimes and soil nutrients monitored in some previously burned areas in hemiboreal forests in Northeast China during 2016–2022

Abstract. Under a warming climate, the occurrence of wildfires has been becoming increasingly more frequent in boreal forests and Arctic tundra over the last few decades. Wildfires can cause radical changes in forest ecosystems and the permafrost environment, such as the irreversible degradation of permafrost, succession of boreal forests, rapid and massive losses of soil carbon stock, and increased periglacial geohazards. Since 2016, we have gradually and more systematically established a network for studying soil nutrients and monitoring the hydrothermal state of the active layer and near-surface permafrost in the northern Da Xing'anling Mountains in Northeast China. Soil moisture content (depth of 0–9.4 m), soil organic carbon content (0–3.6 m), total nitrogen content (0–3.6 m), and total phosphorus and potassium content (0–3.6 m) datasets were obtained in 2016 via field sampling and subsequent laboratory tests. Ground temperature (0–20 m) and active layer thickness (2017–2022) datasets were obtained using thermistor cables that were permanently installed in boreholes or interpolated with these temperatures. The present data can be used to simulate changes in permafrost features under a changing climate and wildfire disturbances and to explore the changing interactive mechanisms of the fire–permafrost–carbon system in hemiboreal forests. Furthermore, they can provide baseline data for studies and action plans to support the carbon neutralization initiative and assessment of the ecological safety and management of the permafrost environment. These datasets can be easily accessed via the National Tibetan Plateau/Third Pole Environment Data Center (https://doi.org/10.11888/Cryos.tpdc.300933, Li and Jin, 2024).

Synergy Between Woody Peat and Bentonite Alters Stability of Soil Organic Carbon in Coarse Soil by Enhancing Capacity for Soil Aggregation and Hydro‐Physical Properties

ABSTRACTCoarse soil has a poor structure and is susceptible to wind and water erosion, thereby making it difficult to maintain the soil organic carbon (SOC) content. Woody peat (WP) is an organic material that can increase the SOC content of the soil, while clay materials can rapidly enhance the capacity for soil aggregate formation. In order to explore the synergistic effects of WP and clay materials (bentonite and red clay) on the aggregate structure and hydro‐physical properties of coarse soil, as well as the mechanism associated with SOC mineralization (ΔSOC), we conducted an incubation study using lou soil (L0) and loessial soil (H0) with three treatments: addition of WP alone (LW, HW), mixture of WP and bentonite (LWB, HWB), and mixture of WP and red clay (LWR, HWR). The three treatments enhanced the proportion of macroaggregate (> 2 mm) and aggregate stability of the two soils, and optimized the water retention and ventilation performance. The highest aggregate stability of LWB and HWB can be attributed to the positive synergistic effect of WP and bentonite, and bentonite was more effective than red clay due to its crystal structure. The results also showed that the ΔSOC values were significantly lower under LWB and HWB than those under WP addition alone and adding the mixture of WP and red clay (p < 0.05). Moreover, partial least squares path modeling analysis showed that the hydro‐physical properties of the two improved soils inhibited SOC mineralization (p > 0.05), whereas particulate organic carbon (POC) content significantly accelerated SOC mineralization (p < 0.01). The synergistic effect of clay materials increased mineral‐associated organic carbon (MAOC), which was beneficial to maintain the long‐term effectiveness of WP. Overall, our results demonstrated that the synergistic use of WP and bentonite enhanced the aggregate stability and hydro‐physical properties of coarse soil and improved SOC storage capacity. These results provide scientific and theoretical guidance to facilitate the rapid improvement of coarse soil through engineering measures in arid and semi‐arid areas with water and fertilizer limitations.

Effect of Himalayan cedar forest longevity on soil properties in the Kumaun Himalaya, Uttarakhand, India

The Himalayan cedar forests in the temperate regions of the Himalaya are crucial for providing ecosystem services such as carbon storage and nutrient maintenance, playing a vital role in combating climate change. This study employs a Dendrochronological approach to examine the impact of Himalayan cedar forest stands on soil properties across five distinct sites in the Kumaun Himalaya. Soil samples were collected from a depth of 0–15 cm and analyzed for total nitrogen (TN), total phosphorus (TP), soil organic carbon (SOC), pH, and C:N ratios. Tree-ring samples were also collected taken from the same sites. Our analysis revealed a positive correlation between tree age and SOC content. Notably, the oldest forests at Jageshwar Dham (480 years) and Hatkalika (413 years) exhibited the highest levels of SOC, TP, and TN, while the youngest forests at Naudunga (135 years) and Patal Bhuwaneshwar (134 years) showed the lowest values. Significant differences in all soil parameters were confirmed through a Tukey post hoc test. Additionally, the relationship between climate and tree growth was analyzed using tree-ring chronologies and meteorological records, indicating that cool and moist spring conditions (February-May) enhance tree growth by increasing soil microbial activity. Our findings emphasize that conserving older natural forests, particularly mature Himalayan cedar forests, is crucial for maintaining soil characteristics, including carbon storage and nutrient levels, to support sustainable soil health and climate change mitigation.

Tree species-dependent effects of afforestation on soil fungal diversity, functional guilds and co-occurrence networks in northern China

Afforestation exerts a profound impact on soil fungal communities, with the nature and extent of these changes significantly influenced by the specific tree species selected. While extensive research has addressed the aboveground ecological outcomes of afforestation, the nuanced interactions between tree species and soil fungal dynamics remain underexplored. This study investigated the effects of afforestation with Caragana microphylla (CMI), Populus simonii (PSI), and Pinus sylvestris var. mongolica (PSY) on soil fungal diversity, functional guilds, and co-occurrence networks, drawing comparisons with neighboring grasslands. Our findings reveal a significant increase in soil fungal Chao1 richness following afforestation, though the degree of enhancement varied across tree species. Specifically, CMI and PSI forests showed notable increases in fungal richness, whereas the response in PSY forests was comparatively modest. Saprotrophic fungal groups, integral to organic matter decomposition, showed a substantial increase across all afforested sites, with CMI forests exhibiting an impressive 205.58% rise. Conversely, pathogenic fungi, which can negatively impact plant health, demonstrated a marked decrease within plantation forests. Symbiotic groups, particularly ectomycorrhizal fungi, were notably enriched solely in PSI forests. Co-occurrence network analysis further indicated that afforestation alters fungal network complexity: CMI forests displayed increased network interactions, while PSI and PSY forests exhibited a reduction in network connectivity. Soil bulk density and organic carbon content emerged as key factors influencing network complexity, whereas tree species identity played a crucial role in shaping soil fungal community composition. Collectively, these results emphasize the importance of adopting a species-specific strategy for afforestation to optimize soil fungal diversity and network structure, ultimately enhancing the ecological resilience and sustainability of forest plantation ecosystems.

The rhizosphere contributes disproportionately to free-living nitrogen fixation in subalpine forest soils

Root activity creates a unique microbial hotspot in the rhizosphere, profoundly regulating free-living nitrogen fixation (FLNF). However, empirical assessments of rhizosphere FLNF and its ecological consequences for nitrogen (N) budgets remain lacking, particularly for different root functional modules. Here, we separately collected rhizosphere soils attached to two root functional modules, absorptive roots and transport roots, and investigated the rates (15N2 incorporation) and regulators of rhizosphere FLNF in a N-limited subalpine coniferous forest. The measured rates were further extrapolated to annual fluxes based on the volume of the rhizosphere and the relationship between FLNF rate and temperature. We found that absorptive roots drove significantly higher rhizosphere FLNF rates than did transport roots (12.2 vs. 7.5 ng N g−1 d−1), with both rhizosphere compartments exhibiting rates well exceeding those in the bulk soil (0.9 ng N g−1 d−1). The FLNF rates were positively correlated with soil organic carbon content but showed no significant relationship with the abundance or composition of the diazotrophic community. Moreover, when extrapolated to the ecosystem level the FLNF fluxes were 2-fold greater in the rhizosphere of absorptive roots than in that of transport roots (0.13 vs. 0.04 kg N ha−1 yr−1). Taken together, despite representing only 6.0% of the soil volume, the two rhizosphere compartments contributed as much as 47.2% to the total soil FLNF fluxes. Overall, we provide empirical evidence that despite its limited volume, the rhizosphere contributes disproportionately to the FLNF in subalpine forest soils. Our findings also underscore the critical role of root functional differentiation in regulating rhizosphere FLNF, which is essential for integrating this process into the ecosystem-level N cycle.

Protective role of reactive aluminum phases to stabilize soil organic matter against long-term cultivation in the humid tropics under volcanic influence

ABSTRACT Cultivation can cause rapid depletion of soil organic matter (SOM), particularly in the humid tropics. Understanding the factors controlling SOM storage is a key step toward effective soil management for sustainable crop production. While clay + silt content is known to be an important factor for soil organic carbon (SOC) storage, recent studies have shown greater importance of oxalate-extracted Al (Alo; roughly corresponds to short-range-order aluminosilicate minerals and organo-Al complexes) and Fe (Feo). However, the extent to which these mineralogical factors control the response of SOM pool to land-use changes remains unclear, especially in the tropics. This study aimed to clarify the factors regulating SOM content in croplands compared to secondary forests or home gardens in Negros Occidental, Philippines. Along two line transects having a wide range of Alo contents, soil samples were collected at a depth of 0 − 10 cm from sugarcane sites (n = 33; long-term continuous cultivation of >70 years) that were paired with either secondary forests (n = 10) or home gardens (n = 20). Sugarcane sites had significantly lower SOC and total nitrogen (N) contents than secondary forest and home garden sites, whereas there was no clear difference between the latter two land uses. Both SOC and total N contents showed significant positive correlations with Alo content and, to a much less extent, with Feo content, but not with clay + silt content. The slope of the regression line between SOC and Alo was indifferent between sugarcane and the other two land uses, while the intercept was significantly lower in the sugarcane sites. These results suggest the protective role of Alo phases in both agricultural and forest soils and the presence of unprotected SOM (e.g. plant litter) in the latter sites. Furthermore, δ13C analysis suggested that the proportion of forest (C3 plant) derived carbon remaining in the sugarcane soils ranged between 10% and 50% and increased with Alo content. Together, our study showed indirect evidence of Alo-induced stabilization of SOM against long-term cultivation under the humid tropical environment.

Organic carbon decomposition temperature sensitivity positively correlates with the relative abundance of copiotrophic microbial taxa in cropland soils

Revealing the relationships between the temperature sensitivity of soil organic matter decomposition (Q10) and microbial communities is critical to predict ecosystem feedbacks to global warming. However, the relationships are still far from well understood, especially within the low latitude regions. To address this knowledge gap, soil samples were collected from >100 cropland sites in Southwest China, a region with highly diverse climatic and environmental conditions. The results showed that Q10 values substantially varied across the region, ranging from 1.85 to 6.81, with an average value of 3.27. They were significantly positively correlated with the contents of soil organic carbon, while negatively correlated with the ratios of soil dissolved organic carbon to total organic carbon content. This indicates that soils with high organic carbon contents are more vulnerable to global warming. Further analysis suggested that Q10 values were positively correlated with soil microbial respiration rates, fungal abundance, prokaryotic diversity, and the relative abundance of copiotrophic microbial lineages, but negatively correlated with the proportions of oligotrophic microbes and microbial co-occurrence network degree. The structural equation modeling analysis suggested that soil organic carbon and its quality, as well as microbial attributes were the main factors explaining the variation in Q10 values. The findings in this study highlight the crucial and complex roles of soil microbiome in determining ecosystem feedbacks to global warming.

Biochar Regulates the Humification of Kitchen Waste and the Effects of the Humic Acid Structure of Products on Black Soil

Kitchen waste is a misplaced resource that is characterized by a high organic matter content, high water content, and a tendency to rot easily. Biochar is a black solid substance produced under high-temperature, anaerobic conditions using agricultural organic wastes as the raw material. It possesses a large specific surface area, a loose and porous structure, and functional groups, which confer high thermal stability and strong adsorption capabilities. However, little is known about how humic products made from biochar affect the composition and structure of soil humus. To solve the above problems, this study carried out a two-year outdoor field experiment by means of element analysis, infrared spectroscopy, and differential thermal analysis (0.4 kg/m2 (W4), 0.8 kg/m2 (W8), 1.2 kg/m2 (W12), 1.6 kg/m2 (W16), and 2.0 kg/m2 (W20)); CK was the blank control (no application). The samples were collected one year and two years after they returned to the field. The results showed that the application of organic materials facilitated the accumulation of soil organic carbon (SOC) and increased the total nitrogen (TN) content. The highest SOC content in the W20 treatment was 12.39 g/kg and 14.67 g/kg in one and two years, respectively. The maximum relative HA content in the W20 treatment was 22.99% one year after returning to the field. The PQ value (the ratio of HA/(fulvic acid (FA) + HA)) for the W20 treatment was 88.21%. The W20 treatment greatly increased the SOC and humus carbon contents. Compared with the CK treatment, all the organic materials applied for one year improved the structure of the humic acid to varying degrees, increased the degree of oxidation, reduced the degree of condensation and thermal stability of the HA in the soil, and gradually simplified the structure of the humic acid; among all the treatments, the W20 treatment had the greatest effect.

Accumulation of soil microbial necromass carbon and its contribution to soil organic carbon after vegetation restoration in the Tibetan Plateau

Vegetation restoration has been proved as an effective strategy to increase soil organic carbon (SOC) sequestration in degraded ecosystems. However, due to different vegetation restoration practices and climate zones, changes of SOC content, dynamics, sources and their controlling mechanisms after vegetation restoration remain inadequately addressed. Taking the advantage of four-year vegetation restoration, we explored the effects of vegetation restoration on SOC content through its fractions, soil aggregates, soil enzymes and microbial necromass contribution to SOC in a desertification region in the Tibetan Plateau (TP). Results showed that vegetation restoration increased SOC contents by 68 % for 0 – 10 cm, 29 % for 10 – 30 cm and 11 % for 30 – 50 cm compared to site without vegetation restoration. Vegetation restoration increased soil macroaggregates (> 0.25 mm) and decreased soil microaggregates (0.53 – 0.25 mm), but increased their associated SOC contents. Meanwhile, vegetation restoration enhanced microbial necromass and its contribution to SOC. Structural equation model revealed that increasing microbial necromass carbon, changing soil aggregates and their associated SOC were the main mechanisms of increasing SOC content after vegetation restoration. Our result further indicated a large potential of SOC sequestration through vegetation restoration in the TP. These findings have important implications for natural based solution for carbon neutrality for China.

Primary productivity regulates rhizosphere soil organic carbon: Evidence from a chronosequence of subtropical Chinese fir (Cunninghamia lanceolata) plantation

Tree plantations worldwide are a large terrestrial carbon sink. Previous studies on the carbon sequestration capacity of plantations mainly focused on tree biomass carbon sequestration, but the importance of soil organic carbon (SOC) was relatively unclear. Living root carbon inputs influence SOC via plant-microbe interactions in the rhizosphere and play an essential role in nutrient cycling. Here, we compared SOC, including its fractions, microbial properties, and major nutrients in rhizosphere and bulk soils, and examined their relationships to net primary productivity (NPP) across three developmental stages of Chinese fir (Cunninghamia lanceolata) plantations (6, 18, and 42 years old) in subtropical China. Although NPP differed among the three plantations, SOC concentration in bulk soils did not vary significantly among them. However, SOC concentration and labile C pool I and recalcitrant C pool in rhizosphere soils were significantly (p < 0.05) higher in the young (6-year) and mature (42-year) plantations, both of which had lower (p < 0.05) NPP (−37.71 % and − 42.67 %) compared to the middle-aged (18-year) plantation, suggesting a decoupling of NPP from rhizosphere SOC in the plantations. The decoupling of NPP from rhizosphere SOC concentrations may be driven by nitrogen (N) and phosphorus (P) tree growth requirements, belowground C allocation, and resultant microbial activity in this highly weathered subtropical soil. Our study provides field-based evidence suggesting that rhizosphere SOC changes are primarily regulated by net primary production in subtropical forest plantations. We propose that accurate predictions of SOC dynamics in forest plantations require an improved understanding of rhizosphere processes during plantation development.

Distribution characteristics of soil active organic carbon at different elevations and its effects on microbial communities in southeast Tibet.

Global mountain ecosystems have garnered significant attention due to their rich biodiversity and crucial ecological functions; however, there is a dearth of research on the variations in soil active organic carbon across altitudinal gradients and their impacts on microbial communities. In this study, soil samples at an altitude of 3,800 m to 4,400 m were collected from Sejira Mountain in the southeast Tibet, and soil active organic carbon components, soil microbial community diversity, composition and structure distribution and their relationships were systematically analyzed. The results revealed a non-linear relationship between the elevation and the contents of soil organic carbon (SOC) and easily oxidized organic carbon (ROC), with an initial increase followed by a subsequent decrease, reaching their peak at an altitude of 4,200 m. The Shannon diversity of bacteria exhibited a significant decrease with increasing altitude, whereas no significant change was observed in the diversity of fungi. The bacterial community primarily comprised Acidobacteria, Proteobacteria, Chloroflexi, and Actinobacteriota. Among them, the relative abundance of Proteobacteria exhibited a negative correlation with increasing altitude, whereas Actinobacteriota demonstrated a positive correlation with elevation. The fungal communities primarily consisted of Basidiomycota, Ascomycota, and Mortierellomycota, with Ascomycota prevailing at lower altitudes and Basidiomycota dominating at higher altitudes. The diversity and composition of bacterial communities were primarily influenced by altitude, SOC, ROC, and POC (particulate organic carbon). Soil carbon-to-nitrogen ratio (C/N), dissolved organic carbon (DOC), and available phosphorus (AP) emerged as key factors influencing fungal community diversity, while POC played a pivotal role in shaping the composition and structure of the fungal community. In conclusion, we believe that soil active organic carbon components had a greater impact on the bacterial community in the primary forest ecosystem in southeast Tibet with the elevation gradient increasing, which provided a theoretical basis for further understanding of the relationship between the microbial community and soil carbon cycle in the plateau mountain ecosystem under the background of climate change.

Biofertilizers: A sustainable strategy for enhancing physical, chemical, and biological properties of soil

A biofertilizer is a biologically derived substance that can enhance soil fertility. It is beneficial for enhancing soil fertility by introducing microorganisms that produce organic nutrients and perhaps decreasing plant illnesses. An extensive review was done to examine the efficacy of several biofertilizers in improving soil properties, including their physical, chemical, and biological characteristics. The secondary data and material for the article were gathered from a variety of sources, including government reports, published research papers, reports from various organizations, and pertinent websites that were examined and their conclusions presented. Although biofertilizers have proven to be a very sustainable method for improving soil quality and increasing crop output, there is a lack of extensive research on their effects on soil’s physical, chemical, and biological aspects. This study aims to offer a comprehensive understanding of various biofertilizers and their effects. This investigation indicated that biofertilizers such as Nitrogen-fixing bacteria, phosphate solubilizers, and potassium solubilizers help to increase NPK content in the soil leading to an increase in the productivity of soil. Moreover, biofertilizers help to enhance soil physical properties (soil bulk density, soil moisture, soil temperature, and soil color), chemical properties (soil pH, soil nitrogen content, soil phosphorus content, soil potassium content, and soil organic carbon content), and chemical properties (population of bacteria, fungi, and actinomycetes). Overall, by overcoming the challenges with biofertilizers in agriculture, we can attain agricultural sustainability.

Soil Organic Carbon Stocks Depend Differently on Physicochemical Features in Subtropical Seasonally Flooded Wetland and Non‐flooded Shoreland Forest

ABSTRACTIn recent years, an increasing number of ecosystems are threatened by seasonal flooding, changing non‐flooded shoreland (NF) into seasonally flooded wetland (SF), but the consequences of this hydrological change for soil organic carbon (SOC) dynamics remain unknown. In this study, we investigated how the SOC content was determined by flooding duration and soil physicochemical variables in adjacent SF and NF at six depths (0–10 cm, 10–20 cm, 20–30 cm, 30–50 cm, 50–70 cm, and 70–100 cm) at Shengjin Lake in subtropical China. Soil physicochemistry and SOC composition were analyzed, and Fourier‐transformed infrared spectroscopy (FTIR) was used to resolve the SOC composition. Neither SOC content nor the vertical distribution of SOC was distinguishable between the sites. However, FTIR data revealed that plant‐originated aliphatics and amides were higher at NF than SF sites, with the opposite pattern for aromatics. At SF sites, SOC content was positively affected by soil moisture and flooding duration and was negatively impacted by soil particle size at most soil layers. At NF sites, SOC content was mainly affected by silt and total Fe at the top 20 cm soil, while a higher fraction of plant‐derived labile C was positively correlated to SOC contents at 30–100 cm depth. The results hence indicated a strong effect of seasonal flooding on SOC dynamics in terrestrial ecosystems. SOC stabilization induced by low mineralization and high adsorption played a central role at SF sites, while SOC formation through plant input was more important at NF sites. Our findings suggest that management strategies designed to conserve SOC will need to be site‐specific.

Identification of dominant drivers of streamflow spatiotemporal variations in typical mountainous areas in the Hexi Corridor, China

Study regionTypical mountain areas in the Hexi Corridor, China. Study focusWater security and ecosystem sustainability of arid inland river basins are highly dependent on upstream streamflow. However, due to the complex geographical environment and limited observation data in the study region, the attribution of spatiotemporal variations in streamflow influenced by climate change and/or human activities remains unclear. Here, we used partial least squares regression (PLSR) and the Budyko framework to unravel the dominant drivers of spatiotemporal variation in streamflow over the past 30 yr. New hydrological insight for the regionPrecipitation, topographic wetness index, slope, forest land, gross primary productivity, hydrological connectivity, soil organic carbon content, silt content, relative relief, NDVI and gravel content dominated spatial variation in streamflow. Temporal variation of streamflow was sensitive to precipitation and land surface. Specifically, increased precipitation and land surface alteration dominated the increase in streamflow in 50 % of the watersheds and the decrease in streamflow in 33 % of them, respectively. Further, land surface alteration was dominated by expansion of agricultural and built-up areas, weakened hydrological connectivity, increased landscape aggregation and forest cover. Controlling agricultural and built-up areas and the scale of afforestation, and focusing on the dynamics of hydrological connectivity and landscape patterns in the upstream reaches are imperative to maintain the security and sustainability of water resources in the arid inland river basins.

The effect of polyvinyl chloride microplastics on soil properties, greenhouse gas emission, and element cycling-related genes: Roles of soil bacterial communities and correlation analysis

Different shapes (membranes and particles) and concentrations (1 % (w/w) and 2 % (w/w)) of polyvinyl chloride (PVC) microplastics (MPs) were investigated to determine their impact on the soil environment. The incorporation of MPs can disrupt soil macroaggregates. Compared with 1 % (w/w) MPs, 2 % MPs resulted in a significant increase in soil organic carbon content. MP particles significantly increased soil CO2 emissions, and CH4 emissions were enhanced by both membrane and particle MPs at high concentrations. Microplastics can alter the abundance of Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteriota, and Firmicutes at the phylum level, and Nocardioides, Rhodococcus and Bacillus at the genus level. MP particles had a more significant impact on soil bacterial communities than MP membranes. The relative abundances of genes involved in the C, N, and P cycles were detected by qPCR, and more remarkable changes were observed in MP membrane treatments. The relative abundance of Vicinamibacteraceae and Vicinamibacterales exhibited a positive correlation with most C/N/P cycle-related genes, whereas Pseudarthrobacter and Nocardioides demonstrated a negative correlation. This study highlights that the influence of MPs on soil parameters is mediated by soil microorganisms, providing insight into the effects of MPs on the soil microenvironment.

STABLE CARBON ISOTOPE FOR EVALUATING SOIL ORGANIC CARBON BETWEEN RICE-CORN ROTATION AND CORN MONOCROPPING

Soil organic carbon (SOC) is a significant component that plays an important role in soil fertility and crop sustainable production. This study investigated SOC in soil profiles of rice-corn rotation and corn monocropping using a stable carbon isotope of SOC (δ13CSOC) technique. The soil samples were collected from two plots of rice-corn rotation and corn monocropping fields. Soil sampling was conducted in Tak province, involving the collection of 25 soil cores per plot down to a depth of 50 cm. Each soil profile was further segmented into ten depth layers: 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm, 25-30, 30-35, 35-40, 40-45, and 45-50 cm. The collected soil samples were then subjected to air drying, pulverization, and sieving through a 200-mesh sieve. The δ13CSOC in rice-corn rotation ranged from -28.41‰ to -23.72‰ while corn monocropping was -24.59‰ to -15.83‰. The results revealed that the values of δ13CSOC for each soil profile gradually increased with soil depth. Since δ13CSOC levels were negatively correlated to SOC. Therefore, SOC decreased with increasing soil depth in both the rice-corn and corn fields. However, significantly higher SOC contents were observed in rice-corn rotation rather than in corn monocropping fields (p &lt; 0.05). SOC contents were higher in soil with rice straw retention than without rice residues. The rotation of rice-corn practice could potentially retain organic carbon in soil better than corn monocropping.

Unraveling the impact of global change on glomalin and implications for soil carbon storage in terrestrial ecosystems

Glomalin-related soil protein (GRSP) is a potential byproduct of arbuscular mycorrhizal fungi (AMF) and a major contributor to the passive soil organic carbon (SOC) pool. Despite its crucial role in SOC storage, we know little about the response of GRSP to anthropogenic global change factors (GCFs). Here, using 530 observations from 107 primary studies, we conducted a global meta-analysis to unravel the effects of multiple GCFs (climate change, plant invasion (PI), wildfire, urbanization, land-use change (LUC), and nutrient addition (nitrogen; N, phosphorus; P, and potassium; K) on two functional GRSP fractions (easily extractable- (EE-) and total- (T-) GRSPs) in terrestrial ecosystems. We found that elevated carbon-dioxide increased T-GRSP by 28%, combined NP addition by 39.9%, and NPK addition by 29.5%. Climate warming and alone N addition increased EE-GRSP solely by 2.4% and 13.6%, respectively, but did not influence T-GRSP. However, urbanization and drought decreased T-GRSP by 26% and 15%, respectively. The LUC from natural ecosystems to cropland decreased T-GRSP by 40%, while afforestation in croplands increased it by 32%. Other GCFs (PI, wildfire, and P) had non-significant effects on GRSP probably because of (i) minor changes in AMF activity and (ii) the counterbalancing of effects by opposite processes. GCF impacts were robust when applied at higher intensities for medium-to-long durations (3–10＋ years) in humid conditions and clay-rich soils. The sandy soils experienced greater T-GRSP losses during LUC. Increases in T-GRSP were positively correlated with AMF-root colonization, soil mean-weight diameter, and SOC content. Further, our structure equation model confirmed that GCFs directly influence SOC by altering AMF-GRSP production and indirectly affecting soil aggregate formation and protection, suggesting that optimizing GRSP production can enhance SOC sequestration.

Elevation, Soil and Environmental Factors Determine the Spatial and Quantitative Distribution of Qinghai Spruce Recruitment Biomass in Mountainous (Alpine) Watersheds

Soil heterogeneity observed in the alpine environment plays a very important role in the growth of forest recruitment. However, the mechanisms by which the biomass accumulation and allocation patterns of forest recruitment respond to such environmental differences are unclear, which hinders a thorough understanding of climate change’s impact on forest biomass. We hypothesized that soil heterogeneity influences the distribution of Qinghai spruce recruitment biomass along with elevation. In the frame of this study, carried out in the northern Tibetan Plateau, forest Qinghai spruce recruitment data were combined with soil data derived from 24 sample plots, while permutation multifactor ANOVA and multiple linear regression were utilized to reveal the characteristics of forest recruits’ above- and below-ground biomass and their allocation patterns in response to soil heterogeneity. According to the results, the soil heterogeneity mainly affected the distribution characteristics of recruits’ above- and below-ground biomass at different elevations, while the recruits’ root–shoot ratio variability was influenced by a combination of soil and other environmental factors. Soil organic carbon (SOC) had the greatest effect on the variability of the above- and below-ground biomass of spruce recruits, with R2 of 0.280 and 0.257, respectively. Soil organic carbon and soil moisture content (SMC) had a significant effect on the variability of the root–shoot ratio, with R2 of 0.168 and 0.165, respectively. Soil total nitrogen (TN) and soil organic carbon were the main influencing factors of the above-ground biomass of forest recruits, with contribution rates of 43.15% and 35.28%, respectively. Soil total nitrogen and soil organic carbon were also the main factors influencing the below-ground biomass of forest recruits, with contribution rates of 42.52% and 37.24%, respectively, and both of them had a positive effect on biomass accumulation, and the magnitude of the influence varied with the elevation gradient. Soil moisture content was the main influence factor of spruce recruits’ root–shoot ratio, with a contribution rate of 54.12%. Decreasing soil moisture content would significantly increase the root–shoot ratio of spruce recruits and promote plants to allocate more biomass to root growth. Changes in elevation not only affected the intensity of the effect of soil factors on spruce recruitment biomass and its allocation pattern but even led to a change in the positive and negative effects.

Paddy straw biochar amended sediment enhances pond productivity, growth and physiological profile of Penaeus vannamei cultured in inland saline nursery ponds.

The growing interest in commercial Inland saline aquaculture has taken momentum across the globe due to the available technologies for aquaculture and the abundant resources of saline groundwater. However, the critical problems in inland saline ponds are degraded soil and imbalanced or deficient nutrients. To address these issues, a 75-day experiment was conducted to explore the effects of Paddy Straw Biochar (PSB) as a sediment amendment on sediment quality, water characteristics, growth parameters, and the well-being of Penaeus vannamei reared in inland saline environments. PSB was applied to the nursery ponds at 1 ton/hectare (Treatment, T1) and 2 ton/hectare (Treatment, T2). The findings of water quality parameters showed a significant increase in K+ and Mg++ with a decrease in ammonia-N levels in biochar-applied ponds. Similarly, the addition of biochar to ponds (T2) resulted in improved sediment characteristics with enhanced water holding capacity (24.75%), increased soil organic carbon content (77.94%), pH levels (0.37 units), cation exchange capacity (43.15%), available potassium (16.3%), and lower bulk density (12.61%) compared to ponds subjected to control conditions at the end of the experiment. Over the experiment, improvements in sediment and water quality parameters followed the T2 > T1 > control trend. Growth characteristics showed a significant rise in the percentage of weight gain (12.398 ± 0.12), SGR (11.80 ± 0.01% day-1) and PER (2.39 ± 0.01) with reduced FCR (1.19 ± 0.00) in biochar-treated ponds (T2), followed by T1 and control. The digestive enzyme activity (amylase) and metabolic enzymes like hepatopancreatic alanine aminotransferase (ALT) and muscle ALT activities were significantly higher in shrimps raised in the biochar-applied ponds. On the other hand, the oxidative stress enzyme superoxide dismutase (SOD) in gills, muscles, and hepatopancreas (HP) exhibited lower values, suggesting reduced oxidative stress due to the biochar amendment of the pond sediment. Overall, the study recommends incorporating PSB at a rate of 2 t/ha into nursery pond sediment to enhance water quality, physiological status, and the growth of P. vannamei juveniles. Moreover, this approach improves soil and water characteristics in saline soils, making it an effective culture practice in degraded environments.