Particle Concentration Research Articles

Stabilizing emulsions by ultrasound-treated pea protein isolate - tannic acid complexes: Impact of ultrasonic power and concentration of complexes on emulsion characteristics

This work aimed to investigate the feasibility of stabilizing oil-in-water (O/W) emulsions by ultrasound-treated pea protein isolate-tannic acid (UPPI-TA) complex. The stability and microstructure of the O/W emulsions were evaluated at different ultrasonic powers (0–1000 W) and UPPI-TA complex concentrations (0.25–2.0 wt%). The contact angle (θ) of UPPI-TA was 59.6°, which was suitable for stabilizing O/W emulsions. At an ultrasonic power of 800 W, the droplet size and creaming index (CI) of emulsions decreased, and the apparent viscosity and interfacial protein adsorption content increased with increasing UPPI-TA concentration. In particular, emulsions with 1.5 % UPPI-TA showed the lowest CI, the highest interfacial protein adsorption content and viscoelasticity, as well as the best storage and thermal stability. These results showed that the suitable modifications of ultrasonic emulsification power and particle concentrations were a new potential approach to stabilize the O/W emulsions by ultrasound-treated pea protein isolated-tannic acid complex.

Effects of microplastic interaction with persistent organic pollutants on the activity of the aryl hydrocarbon and estrogen receptors

Environmental microplastics (MPs) are complex mixtures of plastic polymers and sorbed chemical pollutants with high degrees of heterogeneity, particularly in terms of particle size, morphology and degree of weathering. Currently, limitations exist in sampling sufficient amounts of environmental particles for laboratory studies to assess toxicity endpoints with statistical rigor and to examine chemical pollutant interactions. This study seeks to bridge this gap by investigating environmental plastic particle mimetics and pollutant-polymer interactions by mixing polymer particles with persistent organic pollutants (POPs) at set concentrations over time. Solutions containing combinations of polymers including polystyrene (PS), polypropylene (PP), polyethylene terephthalate (PET), and polyamide (PA) and POPs including 2,3,7,8 -Tetrachlorodibenzo-p-dioxin (TCDD), bisphenol A (BPA), and atrazine, were stirred for up to 19 weeks and monitored using assays to test for aryl hydrocarbon (AhR) and estrogen receptor (ER) activity which are cell signaling pathways impacted by environmental pollutants. TCDD induced AhR activity decreased over time in the presence of PS in a surface area dependent manner. BPA and atrazine also exhibited AhR antagonist activity in the presence of TCDD. The addition of BPA slowed the loss of activity but atrazine did not, suggesting that polymer chemistry impacts interactions with POPs. We also observed potential differences in TCDD sorption with different plastic polymers and that higher concentrations of PS particles may inhibit BPA-induced estrogen receptor activation. These results emphasize the need for additional understanding of how POPs and polymer chemistry impact their interaction and toxicity.

A correlation study of road dust pollutants, tire wear particles, air quality, and traffic conditions in the Seoul (South Korea)

The interdependence of traffic dynamics and air quality on the concentrations of tire wear particles (TWP), heavy metals, and carbon black (CB) in road dust collected from 15 locations in Seoul, South Korea, was assessed. Pearson correlation, principal component analysis, and network analysis were employed to evaluate the correlations among traffic volume, vehicle speed, air quality parameters (PM10, PM2.5, NOx, and CO), and the concentrations of TWP (5934 to 16,253 mg/kg, average 9581 ± 4086 mg/kg), CB (371–22,287 mg/kg, average 4291 ± 6096 mg/kg), and heavy metals (Cr, Mn, Fe, Ni, Cu, Zn, As, Cd, and Pb) in road dust. The enrichment factors for heavy metals and the pollution index of TWP and CB were also calculated to evaluate the contribution of vehicle-derived particulate substances to road dust contamination. It was found that Cluster B, characterized by traffic-related variables such as traffic volume, vehicle speed, and heavy metals (Zn, Cd, and Pb), was significantly correlated with these pollutants, with correlation coefficients reaching up to 0.933. TWP was identified as a significant mediator in the increase of Zn, Pb, and Cd concentrations linked to traffic activities, contributing to pollution levels that were 2–16 times higher than the geochemical background. The presence of TWP and CB in road dust was identified as an indicator of contamination from traffic-related activities, highlighting the importance of Zn, Pb, and Cd as emerging pollutants that require targeted management strategies.

Urea Concentration Detection in Milk Using Microscopic Image Processing Algorithm under Ultraviolet Light Approach

The adulteration of milk is a pressing concern for the citizens of India and people all around the globe. Due to a lack of regulation compliance and insufficient surveillance infrastructure, it is noticeably worse in emerging and slow-growing nations. One of the most common and dangerous adulterants in milk is urea. If the permissible quantity of urea in milk is surpassed, it could have a major negative impact on people's health. All existing methods of urea detection require time, expertise, costly chemicals, and enzymes, along with exorbitant instruments and instrument-specific expertise. The key to overcoming this challenge is having the infrastructure to detect adulterated milk. This study aims to identify a cost- effective and largely implementable system for quantitative detection of urea content to identify adulterated milk primarily for milk distribution centers in India. The proposed milk adulteration detection system, dubbed the MADS, entails a cost-effective, rapid, accurate, precise, and completely novel method for the quantitative computation of urea levels in adulterated milk. It is a device that detects the concentration of particles of urea in milk using a microscopic image processing algorithm under ultraviolet light. Using ultraviolet light and a proprietary program in Python, the isolation of the urea particle from the rest of the milk solids is done and the area concentration, as an average of the value calculated in each of the frames of the video captured through the microscopic camera, is computed. This gives the final urea concentration in milk, which can be used to check whether the concentration follows the government guidelines and exceeds the legal limit.

Molecular and Functional Cargo of Plasma-Derived Exosomes in Patients with Hereditary Hemorrhagic Telangiectasia.

Background: Hereditary Hemorrhagic Telangiectasia (HHT) is a genetic disorder leading to frequent bleeding in several organs. As HHT diagnosis is demanding and depends on clinical criteria, liquid biopsy would be beneficial. Exosomes from biofluids are nano-sized vesicles for intercellular communication. Their cargo and characteristics represent biomarkers for many diseases. Here, exosomes of HHT patients were examined regarding their biosignature. Methods: Exosomes were isolated from the plasma of 20 HHT patients and 17 healthy donors (HDs). The total exosomal protein was quantified, and specific proteins were analyzed using Western blot and antibody arrays. Human umbilical vein endothelial cells (HUVECs) co-incubated with exosomes were functionally examined via immunofluorescence, proliferation, and scratch assay. Results: The levels of the angiogenesis-regulating protein Thrombospondin-1 were significantly higher in HHT compared to HD exosomes. Among HHT, but not HD exosomes, a negative correlation between total exosomal protein and soluble Endoglin (sENG) levels was found. Other exosomal proteins (ALK1, ALK5) and the particle concentration significantly correlated with disease severity parameters (total consultations/interventions, epistaxis severity score) in HHT patients. Functionally, HUVECs were able to internalize both HD and HHT exosomes, inducing a similar change in the F-Actin structure and a reduction in migration and proliferation. Conclusions: This study provided first insights into the protein cargo and function of HHT-derived exosomes. The data indicate changes in sENG secretion via exosomes and reveal exosomal Thrombospondin-1 as a potential biomarker for HHT. Several exosomal characteristics were pointed out as potential liquid biomarkers for disease severity, revealing a possible new way of diagnosis and prognosis of HHT.

Preparation and tribocorrosion behavior of electrodeposited Ni–W/ SiC composite coatings

Ni-W composite coatings that are applied using electrodeposition exhibit excellent mechanical strength, corrosion resistance and wear resistance and are a substitute for hard chrome because they involve fewer environmental hazards than regular chromium plating processes. This study determines the effect of process parameters, such as current density (5, 10 and 15 A/dm2) and SiC concentration (0.5, 1.0 and 1.5 g/L), on the chemical composition, structure, mechanical properties and corrosion resistance of electrodeposited Ni-W/SiC composite coatings and determines the interaction between the mechanical behavior and the electrochemical reactions that occur during corrosion and friction. The experimental results show that there are no cracks on the surface of the coating and that the surface roughness increases as current density increases. As the content of W and SiC particles in the composite coating increases, the hardness and corrosion resistance of the coating increase because of solid solution strengthening and nano-ceramic particle dispersion strengthening.In order to verify the protective performance of the coating in complex environments, a ball-on-disk abrasion tester and a potentiostat are used to determine the tribocorrosion behavior of the Ni-W/SiC composite coating against the sliding of the Al2O3 counter-body. In 3.5 wt%NaCl solution and at +600 mV, the corrosion and wear characteristics of composite coatings that are produce using different process parameters are determined. Analysis of the synergistic effects of corrosion and friction shows that the wear component (△Wwear) is 3–5 times greater than the corrosion component (△Wcorr), which is the main cause of coating damage. The greater the hardness of the coating, the less mass is lost for the wear component (△Wwear). The results show that the operating parameters for producing ideal Ni-W/SiC composite coatings are a SiC particle concentration 1.0 g/L and a current density of 10A/dm2. These settings give the best wear resistance in corrosive environments.

Surface microplastics dynamics in the Persian Gulf and Arabian Sea using numerical modelling and CYGNSS satellite estimations

Microplastics have long-term negative effects on marine environment. One of the most significant threats of microplastics is their ability to absorb chemicals which enhances the transfer of pollutants. These pollutants eventually enter the tissues of living organisms e.g. through ingestion. To shed a light on the way these particles accumulate in the surface water of Persian Gulf and the Arabian Sea and the spatial and temporal distribution of their concentrations, a combination of field sampling, remote sensing techniques, and numerical modelling methods were used. Samples were collected using a Neuston net at 31 stations in 2018 and 2021. A hydrodynamic model was used to study the transport of these materials by tide, wind and density-driven currents, and microplastic pathways were mapped. Also, CYGNSS satellite data were used to estimate the particles concentration by measuring the roughness of the ocean surface. It was shown that the northeastern part of the Arabian Sea had the highest concentration of microplastics in winter. Oman's northern border and the Strait of Hormuz had relatively higher concentrations than other parts. This accumulation increases in winter and continues to rise until the end of summer. In autumn, the accumulation decreases, but it begins to increase again in the north of Oman during winter. During winter, the southern part of the Persian Gulf had high concentration, while from summer to autumn, the concentration in the northwest region had increased. In 2021, the average microplastic concentration in the Arabian Sea and the Gulf of Oman varied seasonally from 2.6x104 to 1.8x104 particle per km2. Meanwhile, the average concentration of pollutants in the Persian Gulf was almost invariable throughout the year, ranging from 2.8 x104 to 2.6 x104 particle per km2. Furthermore, the study reveals that these concentrations are influenced by various environmental factors. In the Persian Gulf, water density is the most significant factor controlling the surface concentration of microplastics, while in the Arabian Sea, the interaction of wind speed and sea surface currents is crucial.

Particle motion characteristics on the rotational flow pneumatic conveying of horizontal-vertical pipeline

In this study, a rotational flow device (rotational blade) is developed and installed in the upstream of the particle inlet with the aim of improving the efficiency and capacity of pneumatic conveying. Firstly, this study analyzed the energy-saving effect of rotational flow based on the pressure drop and power consumption. The results shown that the optimal velocity can be reduced by a maximum of 18.7 % and the power consumption coefficient can be reduced by a maximum of 9.8 %. Furthermore, the distributions of particle concentration, velocity and pulsation velocity are analyzed by using electrical capacitance tomography (ECT) and particle image velocimetry (PIV) system. It is found that the particle velocity and velocity pulsation intensity for rotational flow are higher, and they have the ability to enhance particle suspension. Then, the power spectrum of the particle pulsation velocity shown that the rotational flow exhibited higher peak value at lower frequencies, indicating the particles are not easily deposited at pipe bottom. Finally, the auto-correlation of particle pulsation velocity indicated that the particle motion is more stable and has a longer period at low particle concentrations. The skewness factor and probability density function of particle pulsation velocity indicated that the use of rotational blades makes the particle pulsation velocity to deviate from the Gaussian distribution.

Study on the hydraulic conveyance characteristics of suspended coarse particles within pipelines

The Flow Regime Transition Device transforms the gelled crude oil mass into gelled crude oil particles for hydraulic conveying. The gelled crude oil particles may reaggregate into gelled crude oil mass at the later stage of hydraulic conveying. Therefore, understanding the patterns of hydraulic suspension conveying gelled crude oil particles is vital for safely gathering and efficiently conveying crude oil. This paper employs the CFD-DEM coupling technique to conduct a numerical study on the hydraulic conveying characteristics of gelled crude oil particles. The accuracy of the model and the solution method is verified by comparing them with the experimental data of particle concentration distribution, and single particle rise behavior, and particle transport flow of gelled crude oil. The size range of gelled crude oil particles spans from 0.0001 m to 0.003 m. We operate the transportation within a velocity range of 0.5 to 4 m/s and a concentration range of 0.022 to 0.0537. Simulation results indicate two flow regimes in the hydraulic conveying of gelled crude oil particles: an upper-moving bed flow and a heterogeneous suspension flow. With increased conveying velocity, the upper moving bed flow gradually transitions to a heterogeneous suspension flow. Beneath the upper moving bed flow, some scattered particles exist, likely resulting from the action of turbulent diffusion. Changes in conveying concentration and particle size have a minor impact on the velocity distribution of particles and fluid but a more significant effect on the particle distribution across the pipe cross-section. Also, when conveying particles of different sizes, most smaller particles travel along the pipe wall.

S. hispanica mucilage/ ZnO based films: Preparation, characterization, and potential application

The use of plastic for food packaging is a major contributor to environmental pollution. To address this issue, biopolymeric films have emerged as a potential alternative to plastic products commonly used in food packaging. These biopolymeric films can be enhanced with antimicrobial agents to make them more effective. This study aimed to develop and evaluate the properties of films made from Salvia hispanica mucilage and ZnO particles. The mucilage was obtained by filtering and drying seeds, while ZnO was synthesized using the forced hydrolysis of ZnC₄H₆O₄ and NaOH. The films were produced by combining the mucilage with varying concentrations of ZnO (0.1, 0.5, and 1.0%) using a casting method. Tests were conducted to evaluate the films' physical, chemical, and biological properties. X-ray diffraction analysis showed that ZnO was present, while scanning electron microscopy revealed the presence of C, O, and Zn elements. Fourier transform infrared spectroscopy confirmed the presence of S. hispanica mucilage. Thermogravimetric analysis showed that the films were thermally stable at temperatures below 225 °C. Dynamic mechanical analysis revealed that the 0.1% film had the best resistance compared to the 0.5% and 1.0% films. Finally, the study found that the antimicrobial activity of the films depended on the concentration, size, and morphology of the ZnO particles. In conclusion, further research is needed to create films that can effectively inhibit pathogenic bacteria growth in food and be a viable alternative to plastic for food packaging.

Distribution of oil mist particles and air quality improvement in the working area of the cutting workshop

The oil mist particles cyclically emitted during the cutting process are a serious health hazard for workers in machine plants. Affected by emission parameters and seasonal factors, the dynamic distribution of oil mist particles in the workers' breathing zone is not yet clear, and suitable ventilation optimization is yet to be proposed. This paper investigates the dynamic distribution of oil mist particles in the workers' breathing zone and ventilation optimization by numerical simulation. It was found that the accumulated value, peak value, and duration of peak of the oil mist particle concentration in the breathing zone are 1.5 ∼ 5 x, 3 ∼ 7 x, and 2 ∼ 3 x higher than other conditions, respectively, influenced by the emission parameters of the cutting process. Due to the interference of air conditioning airflow, the concentration is high in winter with obvious periodic fluctuations (7 x), while it is relatively low and stable (2 x) in summer. Parallel flow air curtains can decrease the concentration of oil mist particles in the breathing zone by 42.1 % ∼ 67.9 %, with a 13 % ∼ 50 % increase in energy consumption. This paper provides guidance for clarifying the dynamic distribution of pollutants at workers' post in industrial buildings, as well as the protection of workers' breathing environment.

Parametric study of free turbulent slurry jet: influence of particle size and particle concentration on the flow dynamics and thermal behavior of high-speed slurry jet

A detailed computational investigation was conducted to explore the dynamics of high-speed free slurry jets, with a focus on how variations in abrasive size and concentration affect their behavior. The study yielded several significant observations: Firstly, it was observed that slurry jets containing larger particles exhibited notably higher average velocities, attributed to their inherent self-similar characteristics. Specifically, at the far field of the jet, slurry jets with larger particle sizes demonstrated a 15% increase in velocity compared to those with smaller particles. Additionally, an analysis of turbulent intensity revealed that beyond a certain axial distance (x/D = 10), turbulence levels progressively increased. However, intriguingly, for slurry jets with a high concentration (Co = 15%), there was a notable decrease (28%) in turbulent intensity compared to water jets, indicating a complex interplay between particle size and concentration. Secondly, the study found that as the concentration of particles in the slurry jet increased, there was a corresponding rise in the bulk temperature of the jet. This phenomenon was primarily attributed to the heightened thermal conductivity resulting from the increased density of particles in the water. Furthermore, an examination of the Nusselt number revealed interesting trends. While the Nusselt number exhibited a peak near the nozzle exit, indicative of enhanced heat transfer in this region, it showed a decremental trend along the axial direction of the jet, attributable to jet divergence. In summary, the computational analysis provided valuable insights into the behavior of high-speed slurry jets, highlighting the intricate relationships between abrasive size, concentration, velocity distribution, and thermal characteristics. These findings contribute to a deeper understanding of slurry jet dynamics and have significant implications for various industrial applications.

A novel Pickering emulsion gels stabilized by cellulose nanofiber/dihydromyricetin composite particles: Microstructure, rheological behavior and oxidative stability

Particle concentrations (w) and oil content (Φ) are crucial factors influencing the gel stability of Pickering emulsions. To understand the stabilization mechanism comprehensively, we prepared emulsion gels stabilized by CNF/DMY composite particles at various w (0.5–1.5 wt%) and Φ (0.2–0.6, v/v). The microstructure revealed the adsorption of these particles at the oil-water interface, with excess particles forming a three-dimensional network structure in the continuous phase. Rheological studies showed that the network structure of Pickering emulsions was significantly influenced by w and Φ, resulting in improved emulsion gel strength that hindered the movement of oil droplets and oxygen in the continuous phase, thereby enhancing emulsion stability. Three scenarios for the critical strain (γco) were observed: at Φ = 0.2, γco decreased with increasing w, while at Φ = 0.4, γco increased with increasing w. At Φ = 0.6, γco remained relatively constant regardless of w. In conclusion, adjusting particle concentration and oil content enabled the control of microstructure, rheological properties, and antioxidant capacity of emulsion gels. These findings could be a valuable resource for formulating and ensuring the quality of emulsion gel-based products in the food industry.

Techniques and Instruments for Assessing and Reducing Risk of Exposure to Nanomaterials in Construction, Focusing on Fire-Resistant Insulation Panels Containing Nanoclay.

The paper explains how nano exposure is assessed in the construction field and focuses on the production of fire-resistant insulation panels with nanoclay. Utilizing the commercial ANSYS CFX® software, a preliminary theoretical simulation was conducted on nano exposure in the workplace, which revealed that particle dispersion is primarily driven by diffusion. Panel post-processing through drilling results in the highest inhalation exposure, followed by mixing and grinding activities. Compared to a state of 'no activity', each activity resulted in an exposure increase by a factor of min. 1000. An overall assessment suggests that the use of nanoparticles in construction materials may not significantly heighten workers' exposure to nanopowders when considering particle concentration alone as opposed to using traditional micro-scale materials. However, the issue persists when it comes to blending powders or performing finishing tasks on panels, with concentration levels being significantly higher for drilling, grinding, and mixing powders at 2.4 times above the standard reference value (40,000 particles/cm3); this is unacceptable, even for brief durations. Examination of dermal contact with gloves and masks worn by workers revealed no nanoparticle penetration. Safety measures were proposed for workers based on decision trees to enhance their safety. Ten categories of protection strategies have been devised to combat the impact of nanoparticles, which are tailored to specific technical situations, but they must be modified for various types of nanoparticles despite potential shared health implications.

Performances of two-stage electrostatic precipitator with different types of discharge electrodes of unipolar electrostatic agglomerator

Current-voltage characteristics of electrostatic agglomerator with three types of discharge electrodes, operating as the first stage of a two-stage electrostatic precipitator, and the effects of its operating parameter on the removal efficiency of this system for PM10 particles have been investigated. The results shown that the removal efficiency of the system of this specific design only weakly depends on the supply voltage of the AC kinematic electrostatic agglomerator in the supply voltage range of 18–26 kV. A synergistic effect between the operation of electrostatic agglomerator and electrostatic precipitator was obtained. By this effect, the removal efficiency of the whole system, i.e., the electrostatic agglomerator and electrostatic precipitator operating simultaneously, is higher than that determined as the combination of the removal efficiencies obtained for both of these stages operating alone. The effect of synergy results from the agglomeration of fly ash particles in the electrostatic agglomerator stage that decreases the concentration of small particles and increases their removal efficiency by electrostatic precipitator.

Mechanism of Improving Etching Selectivity for E-Beam Resist AR-N 7520 in the Formation of Photonic Silicon Structures

The selectivity of the reactive ion etching of silicon using a negative electron resist AR-N 7520 mask was investigated. The selectivity dependencies on the fraction of SF6 in the feeding gas and bias voltage were obtained. To understand the kinetics of passivation film formation and etching, the type and concentration of neutral particles were evaluated and identified using plasma optical emission spectroscopy. Electron temperature and electron density were measured by the Langmuir probe method to interpret the optical emission spectroscopy data. A high etching selectivity of 8.0 ± 1.8 was obtained for the etching process. The optimum electron beam exposure dose for defining the mask was 8200 pC/m at 30 keV.

MβCD inhibits SFTSV entry by disrupting lipid raft structure of the host cells

Severe fever with thrombocytopenia syndrome virus (SFTSV), recently named as Dabie bandavirus, belongs to the family Phenuiviridae of the order Bunyavirales, is a newly-identified bunyavirus with a case fatality rate of up to 30%, posing a serious threat to public health. Lipid rafts on plasm membranes are important for the entry of enveloped viruses; however, the role of lipid rafts in bunyavirus entry remains unclear. In this study, we found that methyl-beta-cyclodextrin (MβCD), a drug that disrupts cholesterol in lipid rafts of cell membranes, inhibits SFTSV infection. Additionally, there is a back-complementary effect of SFTSV infection upon the addition of cholesterol. Moreover, the concentration of SFTSV particles in lipid rafts during entry directly indicated the role of lipid rafts as a gateway, whereas MβCD could inhibit SFTSV entry by affecting the structure of lipid rafts. In an in vivo study, MβCD also reduced the susceptibility of mice to SFTSV infection. Our results suggest that SFTSV can interact with Talin1 proteins on lipid rafts to enter host cells by endocytosis of lipid rafts and reveal the potential therapeutic value of MβCD for SFTSV infection.

Volcanic ash in the water column: Cellular, physiological and anatomical implications for the gastropod suspension-feeder Crepipatella peruviana (Lamarck, 1822)

The influx of volcanic ash into seawater alters particle composition with implications for the cellular, physiological and anatomical response of suspension-feeding organisms. Adult females of Crepipatella peruviana were exposed to three diets consisting of a fixed concentration of 50,000 cells ml−1 of the microalga Isochrysis galbana plus different concentrations of ash particles (30, 90 and 150 mg L−1). The objective was to determine the cellular, physiological and anatomical responses. Mortality increased with ash concentrations, while feeding and respiration rates, tissue weight, and condition index decreased. The gills showed severe degradation of cilia and the presence of large mucous aggregates of cilia and ash. An increase in ash resulted in decreased lipid peroxidation and protein carbonyls, but increased total antioxidant capacity and phenols. Thus, volcanic ash particles may exert a high impact at both cellular and physiological levels for C. peruviana, where inhibition of gill function reduces the ability to acquire food.

Non-linear filtration model with splitting front

We consider filtration of a suspension in a homogeneous porous medium which is described by a macroscopic 1-D model including the mass exchange equation and the kinetic equation for deposit growth. The standard model assumes that suspended particles move at the same speed as the carrier fluid. However, in some experiments, a lag between the front boundary of suspended particles and the front of the carrier fluid was observed. This article proposes a modification of the standard model that provides a description of the separation between the fluid front and the particle front. For this purpose, a non-smooth non-linear function depending on the concentration of the suspension is introduced into the deposit growth equation. In case of a non-smooth suspension function, the concentration of suspended particles at the front decreases to zero in a finite time. At this moment the united front splits into the front of pure injected water and the front of suspended and retained particles. The particle front moves slower than the pure water front. In case of a linear filtration function (Langmuir coefficient), an exact solution is constructed in a closed form. For the filtration problem with a suspension function in the form of a square root, explicit analytical formulae are obtained. In case of a non-smooth filtration function, the filtration time is finite. The curvilinear boundary separates the filtration domain, where concentrations increase with time, from the stabilization domain, where the concentrations of suspended and retained particles have reached their limits. The limit speeds of the stabilization border and of the particle front coincide.

Synergistic Role of the AuAg-Fe3O4 Nanoenzyme for Ultrasensitive Immunoassay of Dengue Virus.

A combination of magnetic and noble metal nanoparticles (NPs) has recently emerged as a potential substance for rapid and sensitive immunosorbent assays. However, to make the assay an alternative method for Enzyme-linked immunosorbent assay, the individual role of each nanoparticle must be explored properly. In this work, an immunoassay has been proposed using two antibody-conjugated iron oxide nanoparticles (Fe3O4NPs) and gold-silver bimetallic nanoparticles (AuAgNPs) to enhance the sensitivity of virus detection by colorimetric TMB/H2O2 signal amplification. A synergistic effect is monitored between Fe3O4NPs and AuAgNPs, which is explored for colorimetric virus detection. The sensor exploits the synergistic effect between the nanoparticles to successfully detect a wide range of dengue virus-like particle (DENV-LP) concentrations ranging from 10 to 100 pg/mL with a detection limit of up to 2.6 fg/mL. In the presence of a target DENV-LP, a sandwich-like structure is formed, which restricts the electron transfer and the associated synergistic effect between the nanoparticles, restricting the TMB oxidation process. Therefore, the synergistic effect is the key to the present work, which accounts for the enhanced rate of the enzymatic reaction on TMB and makes the current method of virus detection more sensitive and reliable compared to the others.