Concentrations In Foods Research Articles

Ethylbenzene exposure in North America - an update

Chronic ethylbenzene exposures and attendant potential health risks for United States children and prospective parents were first evaluated under the United States Environmental Protection Agency’s Voluntary Children’s Chemical Evaluation Program. Using updated data and methods, a 2015 reevaluation observed declines in ethylbenzene releases and concentrations in ambient and indoor air. Both assessments identified inhalation as the dominant exposure route and smoking as the greatest contributor, with dietary intake much lower. Children’s exposure concentrations were similar to that of adults, but their intakes were higher. Neither breastfeeding nor toy mouthing was a significant source. This report updates the previous assessments, summarizing current ethylbenzene concentrations in air and foods, exposures during the use of household and consumer products, nationally representative biomonitoring data, including expanded demographic groups, and a new survey of worker exposures in styrene production facilities. General population ethylbenzene exposures appear to have declined for all age groups. The ethylbenzene/styrene chain of commerce contributes an estimated 0.1% to total air emissions and 7%-12% to dietary concentrations. Total estimated ethylbenzene intakes are consistent with biomonitoring data. Lactational transfer is not a significant exposure pathway for breastfed infants. Production workers’ exposure is well below occupational guidelines. Updated exposure estimates for each pathway suitable for potential health risk assessment are proposed.

Comment on “Screening the Multi-element Content of Pleurotus Mushroom Species Using Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES)”

La, Ce, Pr, Nd, Sm, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu are lanthanides, also referred to as “rare earth” elements (occurring at ultra-low concentration, i.e. each, at ppb or lower levels) in plant and animal foods including edible wild mushrooms. Could it be that lanthanides when collectively reported as a summed value (widely referred to as REE) are at relatively high concentrations because extremely high contributions from individual elements? REE elements naturally occur in environmental media such as the soil substrate in which plants and fungi grow in a characteristic pattern (Oddo-Harkins rule), with most of the available literature confirming the extension of this pattern in fungi. Abnormalities therefore need to be examined closely and resolved.

Finger pump microfluidic detection system for methylparaben detection in foods

A novel assay platform consisting of a finger pump microchip (FPM) and a WiFi-based analytical detection platform is presented for measuring the concentration of methylparaben (MP) in commercial foods. In the presented approach, a low quantity (5 μL) of distilled food sample is dripped onto the FPM and undergoes a modified Fenton reaction at a temperature of 40 °C to form a green-colored complex. The MP concentration is then determined by measuring the color intensity (RGB) of the reaction complex using APP software (self-written) installed on a smartphone. The color intensity Red(R) + Green(G) value of the reaction complex is found to be linearly related (R2 = 0.9944) to the MP concentration for standard samples with different MP concentrations ranging from 100 to 3000 ppm. The proposed method is used to detect the MP concentrations of 12 real-world commercial foods. The MP concentrations measurements are found to deviate by no more than 5.88% from the results obtained using a conventional benchtop method. The presented platform thus offers a feasible and low-cost alternative to existing macroscale techniques for measuring the MP concentration in commercial foods.

Interspecific differences in plasma carotenoid profiles in nestlings of three sympatric vulture species.

Carotenoids are diet-based micronutrients important in health and coloration signaling. Related species with similar diets can differ in the kinds and levels of circulating carotenoids, which suggests specific physiological mechanisms to efficiently utilize these micronutrients, regardless of their availability. We explored whether diet and parental provisioning of unusual sources of carotenoids (fresh vegetal matter and vertebrate feces) can explain the occurrence and concentrations of carotenoids in the cinereous vulture Aegypius monachus, griffon vulture Gyps fulvus, and Egyptian vulture Neophron percnopterus nestlings, even when these pigments appear to not be deposited in their integumentary system. A greater diversity of wild prey in diet could be behind the profile of higher concentrations of carotenoids in the Egyptian vulture, the species with carotenoid-dependent coloration during adulthood, while differences in diet composition between cinereous and griffon vultures do not translate to different carotenoid profiles. The carotenoid profile appears to not be related to the ingestion of unusual matter rich in these compounds, although the infrequent occurrence of lycopene and unidentified γ-carotene-like compounds suggest that these vultures may be exploiting vegetal matter that left no identifiable unconsumed remains in the nest of Egyptian vultures. The consumption of green plant material by griffon vultures does not result in especially high levels of carotenoids when compared to the carotenoids found in cinereous vultures, which do not consume green plant material. Ungulate feces were not provisioned to Egyptian vulture nestlings, despite the fact they contain carotenoids that adults need for appropriate coloration. Overall, this study indicates that diet differences alone appear insufficient to explain contrasting interspecific carotenoid profiles, especially since all types of food consumed are considered to be poor in carotenoids, except vegetable matter. We suggest that nestling Egyptian vultures are comparatively efficient in uptaking carotenoids present in low concentrations in food when these compounds are not deposited in their integument, which suggests allocation to other functions.

Residual levels and health risk assessment of trace metals in Chinese resident diet

Large-scale metal contamination across the food web is an intractable problem due to increasing pollutant emissions, atmospheric transport, and dry and wet deposition of elements. The present study focus on several trace metals that are rarely studied but have special toxicity, including tin (Sn), antimony (Sb), gold (Au), hafnium (Hf), palladium (Pd), platinum (Pt), ruthenium (Ru), tellurium (Te) and iridium (Ir). We investigated trace metals residues and distribution characteristics, and further evaluated the potential health risks from major daily food intakes in 33 cities in China. Sn, Sb, Ir, Hf, and Au were frequently detected in food samples with the concentrations ranged from ND (not detected) to 24.78 µg/kg ww (wet weight). Eggs exhibited the highest residual level of all detected metals (13.70 ± 14.70 µg/kg ww in sum), while the lowest concentrations were observed in vegetables (0.53 ± 0.17 µg/kg ww in sum). Sn accounting for more than 50% of the total trace metals concentration in both terrestrial and aquatic animal origin foods. In terrestrial plant origin foods, Sn and Ir were the most abundant elements. Hf and Au were the most abundant elements in egg samples. In addition, Sb and Ir showed a clear trophic dilution effect in terrestrial environments, while in aquatic ecosystems, Sn, Hf, and Au exhibited obvious trophic amplification effects. The calculated average estimated daily intake (EDI) via food consumption in five regions of China was 0.09 µg/(kg·day), implying the health risk of aforementioned elements was acceptable.

Statistical Methods for Handling Nondetected Results in Food Chemical Monitoring Data to Improve Food Risk Assessments

Chemical risk assessment is important for risk management, and estimates of chemical exposure must be as accurate as possible. Chemical concentrations in food below the limit of detection are known as nondetects and result in leftcensored data. During statistical analysis, the method used for handling values below the limit of detection is important. Many risk assessors employ widely used substitution methods to treat left-censored data, as recommended by international organizations. The National Institute of Food and Drug Safety Evaluation of South Korea also recommend these methods, which are currently used for chemical exposure assessments. However, these methods have statistical limitations, and international organizations recommend more advanced alternative statistical approaches. In this study, we assessed the validity of currently used statistical methods for handling nondetects. The best method was determined based on a simulation study. In three case studies, we compared the various methods based on the root mean squared error. The data for all case studies were from the same source, to avoid heterogeneity. Across various sample sizes and nondetection rates, the mean and 95th percentile values for all treatment methods were similar. However, “log normal maximum likelihood estimation” method was not suitable for estimating the mean. Risk assessors should consider statistical processing of monitoring data to reduce uncertainty. Currently used substitution methods are effective and easy to apply to large data sets with nondetection rates < 80%. However, advanced statistical methods are required in some circumstances, and national guidelines are needed regarding their use in risk assessments.

Comparison of measured and declared vitamin D concentrations in Australian fortified foods

Fortified foods are an important source of dietary vitamin D, since this nutrient occurs naturally in relatively low concentrations in a limited number of foods. Hence, we aimed to investigate the accuracy of the declared vitamin D content of Australian fortified foods. Vitamin D3, 25-hydroxyvitamin D3 (25(OH)D3), vitamin D2 and 25(OH)D2 were measured in 30 fortified food samples (edible oil spreads, malted chocolate drink powders, soy milks and breakfast cereals) using liquid chromatography with triple quadrupole mass spectrometry. The measured vitamin D content ranged from − 54 % to + 190 % of declared values. One product had measured vitamin D content close to the declared value, while 10 of 14 products had vitamin D in excess of that declared. Label information proved an unreliable indicator of measured vitamin D content across all product categories, which may be problematic for those relying on fortified foods as their main source of vitamin D.

Organophosphate esters in UK diet; exposure and risk assessment

Food ingestion has been established as an important human exposure route to many environmental contaminants (brominated flame retardants, dioxins, organochlorine pesticides etc). However, information regarding dietary exposure to organophosphate esters (OPEs) in the UK remains limited. This study provides the first comprehensive dataset on OPEs in the UK diet by measuring concentrations of eight OPEs in 393 food samples, divided into 15 food groups, collected from Birmingham, UK. All target OPEs were measured above the limit of quantification in at least one of the food groups analysed. Concentrations were highest (mean ∑8OPEs = 18.4 ng/g wet weight (ww)) in milk and milk products, followed by those in cereal and cereal products (mean ∑8OPEs = 15.9 ng/g ww), with concentrations lowest in chickens' eggs (mean ∑8OPEs = 1.61 ng/g ww). Interestingly, concentrations in animal-derived foods (mean ∑8OPEs = 44.2 ng/g ww) were statistically indistinguishable (p˃0.05) from plant-derived foods (mean ∑8OPEs = 36.8 ng/g ww). Estimated daily dietary intakes (EDIs) of ∑8OPEs under mean and high-end exposure scenarios for the four age groups considered were: toddlers (420 and 1547 ng/kg bw/day) ˃ children (155 and 836) ˃ elderly (74.3 and 377) ˃ adults (62.3 and 278) ng/kg bw/day, respectively. Baby food contributed 39 % of ∑8OPEs exposure for toddlers, with non-alcoholic beverages contributing 27 % of exposure for children, while cereal and cereal products (25 %) and fruits (22 %) were the main contributors for adults and the elderly. The concentrations of OPEs in UK foodstuffs were generally of the same order of magnitude as those reported for other countries and our estimates of dietary exposure were well below the corresponding health-based limit values.

Light-shading reaction microfluidic PMMA/paper detection system for detection of cyclamate concentration in foods

Cyclamate is an artificial sweetener with high sweetness and low calories, and is a common sugar substitute for weight control and diabetic patients. However, excessive cyclamate consumption is associated with various health disorders, and hence it is prohibited as a food additive in many countries around the world. The current research proposes a light-shading reaction microfluidic PMMA/paper detection (MPD) system for determining the cyclamate concentration in food. In the current system, inject 10 μL of the extracted sodium cyclamate sample into the sample chamber of the MPD device, perform the diazotization reaction under shading conditions, and then suck it into the detection area through a paper strip, which consists of a paper chip embedded with modified Bratton-Marshall reagent. Once the paper chip is thoroughly wetted, the MPD device is inserted into a microanalysis box, where a fuchsia azo reaction compound is produced through heating at 40 °C for 3 min. The reaction complex is observed by a camera and the reaction image is wirelessly transmitted to a smartphone, and the concentration of sodium cyclamate is measured through the self-developed grayscale software. The results obtained for the sodium cyclamate samples with a concentration in the range of 50–1000 ppm show that the measured gray value changes linearly with the sodium cyclamate concentration, and the correlation coefficient (R2) is 0.9898. By analyzing the concentration of sodium cyclamate in 10 real-world samples, the practical feasibility of the current MPD system is proved. The results showed that the concentration measurement value did not deviate by more than 4.8 % from the value obtained using the conventional liquid chromatography/tandem mass spectrometry (LC-MS/MS) method.

Risk assessment of mercury through dietary exposure in China

Mercury (Hg) is a widespread heavy metal causing various damages to health, while insufficient studies assessed its exposure risk across China. This study explored concentrations in food items and dietary exposure risks across China by comprehensively analyzing the researches on total Hg (THg) in eight food items and methylmercury (MeHg) in aquatic foods published between 1980 and 2021. According to the included 695 studies, the average THg concentration in all food items was 0.033 mg/kg (ranging from 0.004 to 0.185 mg/kg), with the highest concentration in edible fungi. The average daily dietary THg exposure from all foods was 12.9 μg/day. Plant-based foods accounted for 62.7% of the dietary THg exposure. Cereals and vegetables were the primary source of THg exposure. The MeHg concentration in aquatic foods was 0.08 mg/kg, and the average dietary exposure was 3.8 μg/day. Monte Carlo simulations of the dietary exposure risk assessment of THg and MeHg showed that approximately 6.4 and 7.0% of residents exceeded the health-based guidance value set by the European Food Safety Authority, with higher exposure risk in Southwest and South China. The nationwide target hazard quotient index of THg was greater than 1, suggesting that the non-carcinogenic risk of dietary exposure to THg needed further concern. In summary, this study has a comprehensive understanding of dietary Hg exposure risks across China, which provide a data basis for Hg exposure risk assessment and policy formulation.

Pilot study on the effect of secondary copper smelters on polychlorinated naphthalene contamination in surrounding agricultural areas

Polychlorinated naphthalenes (PCNs) are dioxin-like persistent organic pollutants that are primarily produced unintentionally during industrial thermal processes. These compounds are harmful to the environment and human health. This study investigated the occurrences of all 75 PCN congeners in agricultural areas near secondary copper smelters in China. The PCN concentrations in aquatic foods, eggs, crops, sediments and soils within 10 km of these smelters were higher than those in samples collected 20–30 km away from such facilities. In contrast, the PCN concentrations in compound animal feed samples collected at different distances from the secondary copper smelters were comparable to one another. Similar PCN homologue patterns were found in crop, egg, feed and soil samples collected at different distances from the smelters but the homologue profiles of PCNs in aquatic food and sediment samples collected from different distances varied. Lower chlorinated naphthalenes were the predominant homologues in most samples. The contamination of farm animals and crops with PCNs might result from the emission of these compounds from such sources into the surrounding environment. However, the health risks associated with human exposure to PCNs through food consumption are low for both distances (<10 km and 20–30 km) from secondary copper smelters.

Picomolar glutathione detection based on the dual-signal self-calibration electrochemical sensor of ferrocene-functionalized copper metal-organic framework via solid-state electrochemistry of cuprous chloride

Chemical biosensing techniques are essential for food analysis and disease diagnosis. Nanomaterials with redox activity show great potential in electrochemical analysis, acting as signal labels or signal amplification unit, which can reflect the targets concentration in foods and biological samples. Here, an ultra-sensitive dual-signal intrinsic self-calibration electrochemical platform for GSH was firstly fabricated based on the novel electroactive nanomaterial of ferrocene-functionalized copper metal–organic framework (Fc-Cu-MOF). Due to the solid-state electrochemical property of cuprous chloride (CuCl), a sharp characteristic peak with an increased signal appears with the coexistence of chloride ions in solution. The stronger specific affinity between Cu+ and GSH than that of Cu+ and Cl- triggers a “crowding effect” that causes the current signal of CuCl decrease greatly. Meanwhile, the peak current of ferrocene keeps unchanged as an internal reference. Based on the ratio of the peak current variation (ΔICu/ΔIFc) as the signal output, Fc-Cu-MOF modified electrode showed wider linear range in 0.1 nM −1 μM for GSH with the detection limit as low as 0.025 nM. And the sensor was successfully applied in the determination of GSH with excellent recoveries in various real samples such as food and serum samples, providing good prospect in application of bioanalysis and food screening.

Hydroxytyrosol in Foods: Analysis, Food Sources, EU Dietary Intake, and Potential Uses

Hydroxytyrosol (HT) is a phenolic compound with proven biological properties present in a limited number of foods such as table olives, virgin olive oil (VOO) and wines. The present work aims to evaluate the dietary intake of HT in the European (EU) population by compiling scattered literature data on its concentration in foods. The consumption of the involved foods was estimated based on the EFSA Comprehensive European Food Consumption Database. The updated average contents of HT are as follows: 629.1, 5.2 and 2.1 µg/g for olives, olive oil and wine, respectively. The HT estimated intake in the European Union (EU) adult population falls within 0.13–6.82 mg/day/person, with table olives and wine being the main contributors. The estimated mean dietary intake of HT in EU countries is 1.97 ± 2.62 mg/day. Greece showed the highest HT intake (6.82 mg/day), while Austria presented the lowest (0.13 mg/day). Moreover, HT is an authorized novel food ingredient in the EU that can be added to different foods. Since the estimated HT intake is substantially low, the use of HT as a food ingredient seems feasible. This opens new possibilities for revalorizing waste products from olive oil and olive production which are rich HT sources.

Analytical strategies for the determination of biogenic amines in dairy products.

Biogenic amines (BA) are mainly produced by the decarboxylation of amino acids by enzymes from microorganisms that emerge during food fermentation or due to incorrectly applied preservation processes. The presence of these compounds in food can lead to a series of negative effects on human health. To prevent the ingestion of high amounts of BA, their concentration in certain foods needs to be controlled. Although maximum legal levels have not yet been established for dairy products, potential adverse effects have given rise to a substantial number of analytical and microbiological studies: they report concentrations ranging from a few mg/kg to several g/kg. This article provides an overview of the analytical methods for the determination of biogenic amines in dairy products, with particular focus on the most recent and/or most promising advances in this field. We not only provide a summary of analytical techniques but also list the required sample pretreatments. Since high performance liquid chromatography with derivatization is the most widely used method, we describe it in greater detail, including a comparison of derivatizing agents. Further alternative techniques for the determination of BA are likewise described. The use of biosensors for BA in dairy products is emerging, and current results are promising; this paper thus also features a section on the subject. This review can serve as a helpful guideline for choosing the best option to determine BA in dairy products, especially for beginners in the field.

Arsenic Exposure via Contaminated Water and Food Sources

Arsenic poisoning constitutes a major threat to humans, causing various health problems. Almost everywhere across the world certain “hotspots” have been detected, putting in danger the local populations, due to the potential consumption of water or food contaminated with elevated concentrations of arsenic. According to the relevant studies, Asia shows the highest percentage of significantly contaminated sites, followed by North America, Europe, Africa, South America and Oceania. The presence of arsenic in ecosystems can originate from several natural or anthropogenic activities. Arsenic can be then gradually accumulated in different food sources, such as vegetables, rice and other crops, but also in seafood, etc., and in water sources (mainly in groundwater, but also to a lesser extent in surface water), potentially used as drinking-water supplies, provoking their contamination and therefore potential health problems to the consumers. This review reports the major areas worldwide that present elevated arsenic concentrations in food and water sources. Furthermore, it also discusses the sources of arsenic contamination at these sites, as well as selected treatment technologies, aiming to remove this pollutant mainly from the contaminated waters and thus the reduction and prevention of population towards arsenic exposure.

Organophosphate ester concentrations in infant food and dietary risk assessment for the infant population in China

Organophosphate esters (OPEs) are extensively used as plasticizers and flame retardants, whereas information on OPEs in infant foods is limited. In this study, twelve OPEs were investigated in 207 infant food samples collected from ten provinces in China. Mean concentrations of ∑OPEs found in cereal-based complementary foods (5.59 ± 5.54 ng/g) were higher than in infant formulas (2.68 ± 1.86 ng/g) under the upper bound (UB) hypothesis (unpaired t-test, p < 0.05). TEP, EHDPP, TCEP, and TDCPP were found to be the dominant OPEs in infant foods. The mean estimated dietary intakes (EDIs) of the OPEs were 50.2 ng/kg bw/day for infants aged under three years. The mean and 95th percentile hazard index (HI) of OPEs under the UB hypothesis were 6.6 × 10−3 and 1.9 × 10−2, respectively. The dietary risk assessment results showed that the exposure of Chinese infants to OPEs does not indicate a health concern.

The use of kidney bean flour with intact cell walls reduces the formation of acrylamide in biscuits

Acrylamide (AA) is formed by Maillard Reaction (MR), during high-temperature and low moisture processes in several food categories, including bakery products. AA is classified as a toxic and carcinogenic compound and therefore, mitigation strategies are necessary to control its concentration in foods. Selecting ingredients with a reduced amount of asparagine, the main AA precursor, is the most effective method of reducing the AA formation in bakery products.In the present study, the hypothesis that cotyledon cell walls integrity could prevent the formation of AA by modulating the dehydration rate and reducing the availability of asparagine during the MR development during baking was investigated. Legumes have strong cotyledon cell walls that can be preserved during flour production: in this study two kidney bean flours with intact and broken cell walls were used. The integrity of the cotyledon cell walls was assessed by scanning electron microscopy while AA, its precursors, moisture and water activity were measured in bean flours, raw doughs and baked biscuits. The biscuits were also characterised for the main baking parameters such as weight loss, pH, colour and texture.The results showed that biscuits formulated with bean flour with intact cell walls had a 15% less amount of AA than those made with the bean with damaged cell walls at the end of the cooking time. Furthermore, the use of the two different bean flours led to different quality characteristics of the biscuits in terms of hue angle and browning index, as colour parameters, and hardness, which is related to the texture properties. These differences were attributed to the different development of the MR and in the structure of the biscuit during baking. The use of intact plant tissue legume flours in alterative biscuits formulations is a feasible and low-cost approach that can contribute to the AA mitigation strategy.

Application of Molecularly Imprinted Polymers for the Detection of Volatile and Off-Odor Compounds in Food Matrices.

Molecularly imprinted polymers (MIPs) are synthetic receptors having specific cavities intended for a template molecule with a retention mechanism that depends on molecular recognition of the targeted constituent. They were initially established for the detection of minor molecules including drugs, pesticides, or pollutants. One of the most remarkable areas where MIPs have potential utilization is in food analysis, especially in terms of volatile compounds which are found in very low concentrations in foods but play a crucial role for consumer preference and acceptance. In recent years, these polymers have been used extensively for sensing volatile organic and off-odor compounds in terms of food quality for selective high-extraction purposes. This review first summarizes the basic principles and production processes of MIPs. Second, their recent applications in the separation, identification, and quantification of volatile and off-odor compounds in food samples are elucidated.

Challenges in Gluten Analysis: A Comparison of Four Commercial Sandwich ELISA Kits.

Gluten is composed of prolamin and glutelin proteins from several related grains. Because these proteins are not present in identical ratios in the various grains and because they have some differences in sequence, the ability to accurately quantify the overall amount of gluten in various food matrices to support gluten-free labeling is difficult. Four sandwich ELISAs (the R-Biopharm AG R5 RIDASCREEN®, the Neogen Veratox® R5, the Romer Labs AgraQuant® G12, and the Morinaga Wheat kits) were evaluated for their performance to quantify gluten concentrations in various foods and ingredients. The Morinaga and AgraQuant® G12 tests yielded results comparable to the two R5 kits for most, but not for certain, foods. The results obtained with the Morinaga kit were lower when compared to the other kits for analyzing powders of buckwheat and several grass-based products. All four kits were capable of detecting multiple gluten-containing grain sources including wheat, rye, barley, semolina, triticale, spelt, emmer, einkorn, Kamut™, and club wheat. Users of the ELISA kits should verify the performance in their hands, with matrices that are typical for their specific uses. The variation in results for some food matrices between test methods could result in trade disputes or regulatory disagreements.

Attenuation mechanisms of arsenic induced toxicity and its accumulation in plants by engineered nanoparticles: A review

The excessive arsenic (As) accumulation in plant tissues enforced toxic impacts on growth indices. So, the utilization of As-contaminated food leads to risks associated with human health. For the reduction of As concentrations in foods, it is obligatory to fully apprehend the take up, accretion, transportation and toxicity mechanisms of As within plant parts. This metalloid impairs the plant functions by disturbing the metabolic pathways at physio-biochemical, cellular and molecular levels. Though several approaches were utilized to reduce the As-accumulation and toxicity in soil-plant systems. Recently, engineered nanoparticles (ENPs) such a zinc oxide (ZnO), silicon dioxide or silica (SiO2), iron oxide (FeO) and copper oxide (CuO) have emerged new technology to reduce the As-accumulation or phytotoxicity. But, the mechanistic approaches with systematic explanation are missing. By knowing these facts, our prime focus was to disclose the mechanisms behind the As toxicity and its mitigation by ENPs in higher plants. ENPs relives As toxicity and its oxidative damages by regulating the transporter or defense genes, modifying the cell wall composition, stimulating the antioxidants defense, phytochelatins biosynthesis, nutrients uptake, regulating the metabolic processes, growth improvement, and thus reduction in As-accumulation or toxicity. Yet, As-detoxification by ENPs depends upon the type and dose of ENPs or As, exposure method, plant species and experimental conditions. We have discussed the recent advances and highlight the knowledge or research gaps in earlier studies along with recommendations. This review may help scientific community to develop strategies such as applications of nano-based fertilizers to limit the As-accumulation and toxicity, thus healthy food production. These outcomes may govern sustainable application of ENPs in agriculture.