Strain Concentration Research Articles

Microstructural evolution and mechanical response of micro-deformation diffusion bonding Ti-6Al-4V with designed interface morphology

This study explicates the microstructural evolution and mechanical response of the Ti-6Al-4 V joints diffusion-bonded with a micro-deformation less than 1%, assisted by pre-designed interface morphology. The interfaces composed of interfacial voids and bonded zones were developed, exhibiting varying bond rates. The interfacial voids evolved from valleys of bonding surfaces, and the bonded zones were derived from comparatively deformed ridges mainly depending on dynamic recrystallization, strain-induced migration of grain boundaries, and diffusion mechanism. The recrystallized α grains was generated due to the nucleation and rotation of sub-grains driven by pile-up of dislocations. Additionally, the diffusion of elements was significantly activated at the deformed zones (herein, referring to the bonded zones), promoting the metallurgical bonding of α / β phase interfaces. The formation of bonded zones supported for sound joining equivalent to the substrate in mechanical properties, confirmed by the void-free joint. Nevertheless, the mechanical properties of the joints were decreased when interfacial voids led to premature failure, and deteriorated more severely with narrowing void spacing. The concentration of stress and strain at void tips induced untimely initiation of cracks, and propagation guided by the trajectory of high stress. The interaction of stress field surrounding adjacent voids was enhanced under narrow spacing (less than 4 times of void length), leading to acceleration of crack initiation and propagation.

Strain concentration drives the anatomical distribution of injury in acute and chronic traumatic brain injury.

Brain tissue injury caused by mild traumatic brain injury (mTBI) disproportionately concentrates in the midbrain, cerebellum, mesial temporal lobe, and the interface between cortex and white matter at sulcal depths 1-12. The bio-mechanical principles that explain why physical impacts to different parts of the skull translate to common foci of injury concentrated in specific brain structures are unknown. A general and longstanding idea, which has not to date been directly tested in humans, is that different brain regions are differentially susceptible to strain loading11,13-15. We use Magnetic Resonance Elastography (MRE) in healthy participants to develop whole-brain bio-mechanical vulnerability maps that independently define which regions of the brain exhibit disproportionate strain concentration. We then validate those vulnerability maps in a prospective cohort of mTBI patients, using diffusion MRI data collected at three cross-sectional timepoints after injury: acute, sub-acute, chronic. We show that regions that exhibit high strain, measured with MRE, are also the sites of greatest injury, as measured with diffusion MR in mTBI patients. This was the case in acute, subacute, and chronic subgroups of the mTBI cohort. Follow-on analyses decomposed the biomechanical cause of increased strain by showing it is caused jointly by disproportionately higher levels of energy arriving to 'high-strain' structures, as well as the inability of 'high strain' structures to effectively disperse that energy. These findings establish a causal mechanism that explains the anatomy of injury in mTBI based on in vivo rheological properties of the human brain.

Microstructure evolution and mechanical properties of additively manufactured Ni-based GH4099 superalloy via hot isostatic pressing and heat treatment

In this study, GH4099 alloys with over 99.99 % density were produced by laser powder bed fusion (LPBF) and followed by hot isostatic pressing (HIP) and heat treatment (HT). The effects of HIP and HT on the microstructure evolution and mechanical properties of the additively manufactured GH4099 superalloy were examined systematically. The as-deposited columnar crystals were effectively transformed into equiaxed crystals via HIP and HT. During HIP, the elimination of defects and elemental segregation in the deposited samples occurs. However, uneven precipitation of the γ′ phase within grains causes the emergence of minor gaps in the γ matrix, which adversely affects mechanical properties. The microstructure resulting from HIP + solution treatment (ST) consists entirely of equiaxed recrystallized grains. The presence of twin and long-period stacking-ordered (LPSO) phases at twin boundaries strongly hinders dislocation behavior, and the remelting of precipitates and carbides greatly improves plasticity. Moreover, during the loading process at 900 °C, γ′ phase and carbides were re-precipitated, and the strain concentration was minimized during the precipitation process, resulting in the highest high-temperature strength. Conversely, in the HIP + solution and aging treatment (SAT) process, the progression of γ′ phase precipitation, condensation, annexation, and growth contributes to a reduction in strength. Coupled with an increase in the plasticity of the LPBF GH4099 alloy.

Adding mechanobiological cell features to finite element analysis of an immediately loaded dental implant.

Finite element analysis (FEA) has been used to analyze the behavior of dental materials, mainly in implantology. However, FEA is a mechanical analysis and few studies have tried to simulate the biological characteristics of the healing process of loaded implants. This study used the rule of mixtures to simulate the biological healing process of immediate implants in an alveolus socket and bone-implant junction interface through FEA. Three-dimensional geometric models of the structures were obtained, and material properties were derived from the literature. The rule of mixtures was used to simulate the healing periods-immediate and early loading, in which the concentration of each cell type, based on in vivo studies, influenced the final elastic moduli. A 100 N occlusal load was simulated in axial and oblique directions. The models were evaluated for maximum and minimum principal strains, and the bone overload was assessed through Frost's mechanostat. There was a higher strain concentration in the healing regions and cortical bone tissue near the cervical portion. The bone overload was higher in the immediate load condition. The method used in this study may help to simulate the biological healing process and could be useful to relate FEA results to clinical practice.

Experimental and numerical studies of mechanical performance of an assembled friction type structural underpinning joint

This paper proposed an assembled underpinning joint (UPJ) based on the interfacial friction action between the underpinned concrete member and the underpinning steel beams (UPSBs). To investigate the mechanical performance and failure modes of the proposed UPJ, 20 specimens with different concrete strengths were fabricated and tested with varied preloading levels. Experimental results showed that the proposed UPJs could effectively transfer the vertical loads in the concrete columns through the friction action and all specimens failed due to the slip between the underpinned concrete column and the UPSBs. The failure loads of the specimens were in a positively linear relationship with the preloading levels and an interfacial friction coefficient of 0.46 was found. Numerical models were also created to further examine the detailed structural behaviours of each components in the UPJs, which confirmed that, the UPSBs were subjected to a composite action mainly combining both bending and torsion. The frictional stresses between the UPSBs and the concrete column were concentrated in the regions of the web of UPSBs near flanges and stiffeners. The load transfer mechanisms of the proposed UPJs under complex loading conditions were clarified. The damages, occurred at the corners of concrete columns due to high stress and strain concentrations, should be alleviated or prevented by protective measures in practice.

Understanding Stimulation of Conjugal Gene Transfer by Nonantibiotic Compounds: How Far Are We?

A myriad of nonantibiotic compounds is released into the environment, some of which may contribute to the dissemination of antimicrobial resistance by stimulating conjugation. Here, we analyzed a collection of studies to (i) identify patterns of transfer stimulation across groups and concentrations of chemicals, (ii) evaluate the strength of evidence for the proposed mechanisms behind conjugal stimulation, and (iii) examine the plausibility of alternative mechanisms. We show that stimulatory nonantibiotic compounds act at concentrations from 1/1000 to 1/10 of the minimal inhibitory concentration for the donor strain but that stimulation is always modest (less than 8-fold). The main proposed mechanisms for stimulation via the reactive oxygen species/SOS cascade and/or an increase in cell membrane permeability are not unequivocally supported by the literature. However, we identify the reactive oxygen species/SOS cascade as the most likely mechanism. This remains to be confirmed by firm molecular evidence. Such evidence and more standardized and high-throughput conjugation assays are needed to create technologies and solutions to limit the stimulation of conjugal gene transfer and contribute to mitigating global antibiotic resistance.

Finite Element Analysis of Anterior Implant-Supported Restorations with Different CAD-CAM Restorative Materials.

Due to the lack of literature concerning the selection of crown materials for the restoration of anterior teeth, this study aimed to investigate the effects of six distinct computer-aided design and computer-aided manufacturing (CAD-CAM) crown materials on stress and strain distribution within implant-supported maxillary central incisor restorations, employing finite element analysis (FEA). Furthermore, a comparative analysis was conducted between models that incorporated adjacent natural teeth and those that did not, intending to guide the selection of the most suitable modeling approach. Crown materials, including Lava Ultimate, Enamic, Emax CAD, Suprinity, Celtra Duo, and Cercon xt ML, were the subjects of the investigation. FEA models incorporating Coulomb friction were developed. These models were subjected to an oblique load, simulating the average maximum bite force experienced by anterior teeth. The potential for failure in titanium implant components and the prosthesis crown was evaluated through von Mises and principal stress, respectively. Furthermore, the failure of crestal bone was assessed through principal strain values. Stress values for each implant component and strain values of the bone were extracted from the models. To assess the impact of the six groups of crown materials, Kruskal-Wallis analysis of variance and post-hoc comparisons were conducted. Additionally, a statistical comparison between the two groups with Lava Ultimate and Cercon xt ML was performed using the Mann-Whitney U test to determine the difference in the two modeling approaches. Higher crown material stiffness led to decreased stress in the abutment, fixture, and retaining screw, along with reduced strain in the surrounding bone. However, the decrease in stress and strain values became less significant with increasing crown stiffness. Additionally, the model with adjacent teeth showed significantly lower stress and strain concentrations compared to the model without adjacent teeth. Crowns with a high elastic modulus were the optimal choice for anterior teeth restoration. Constructing FEA models with adjacent teeth was highly recommended to gain a deeper understanding of the mechanical behavior of dental implant restorations.

Characterizing damage evolution in fiber reinforced composites using in-situ X-ray computed tomography, deep machine learning and digital volume correlation (DVC)

In-situ X-ray computed tomography (CT) techniques enable to examine microstructural evolution of composite materials continuously and nondestructively under loading for better understanding the initiation and propagation of microscopic damages. This study explores the tensile damage behavior of notched woven carbon/epoxy composites under successive loading process. Since the image contrast of different composite material phases is normally low and conventional image segmentation techniques are hard to identify microstructures and defects in different directions distinctively, a U-Net image segmentation model based on deep machine learning algorithms is employed to characterize microstructures and material damages precisely and consistently. Digital volume correlation (DVC) approach is proposed here tocharacterize the three-dimensional (3D) deformation fields of carbon/epoxy composites at different loading steps, and the DVC results are used to predict location of damage initiation and propagation. It is found that the damage modes of notched carbon/epoxy composites mainly include fiber breakage, longitudinal cracks in warp fiber tows, and transverse cracks in weft fiber tows. The individual fiber breakage occurs in warp fiber tows at the intermediate loading level, and longitudinal and transverse cracks appear in further loading levels. Longitudinal cracks and fiber breakage events take place in a region tangent to the notch tip and exhibit a strong interaction. The development of longitudinal cracks promotes the initiation and propagation of transverse cracks. In addition, the strain concentration regions in 3D deformation fields match well with the microscopic cracks segmented by the U-Net deep learning model. This study demonstrated that the integration of X-ray computed tomography, digital volume correlation and image semantic segmentation enables to identify damage initiation, evolution, extent and mechanism.

Modelling of microscopic crack initiation behaviour of fretting fatigue based on X-ray micro-computed tomography scan

Fretting fatigue is a particularly destructive form of fatigue failure that has drawn significant attention from researchers. This paper proposes a numerical modelling method based on tomography scan to investigate the impact of inclusions on fretting fatigue crack initiation behaviour. The results reveal that inclusions can affect the crack initiation direction and reduce the initiation lifetime. The difference between the properties of inclusions and matrix leads to local mechanical response mismatch, resulting in local stress, strain, and damage concentration. The mismatch in strain and damage follows a gradient distribution along the thickness as the distribution in these mechanical responses. These could explain why particles closer to the surface are more likely to cause damage. Additionally, the aggregation distribution of inclusions results in merging and connecting local regions with high stress, strain, and damage concentration, ultimately promoting fracture behaviour. Finally, the numerical models are validated with experimental data from fretting fatigue tests. Compared to the homogeneous model, the numerical model that considers inclusions from topography data can more accurately analyse the material damage and provide a more comprehensive understanding of fretting fatigue behaviour.

Tensile behavior of rebar-reinforced coarse aggregate ultra-high performance concrete (R-CA-UHPC) members: Experiments and restrained shrinkage creep effect

Rebar-reinforced coarse aggregate ultra-high-performance concrete (R-CA-UHPC) has been used in the construction of new structures and strengthening of deteriorated aged infrastructures, and it inevitably sustains tension. To study the tensile behavior of R-CA-UHPC members, axial tensile tests for dog-bone-shaped specimens were designed and conducted. The investigated variables included reinforcement ratio in terms of rebar quantity/diameter, and concrete type (CA-UHPC vs. normal concrete). The test results showed that the improved rebar/CA-UHPC bond property prevents the emergence of splitting cracks, but intensifies the crack localization for CA-UHPC and strain concentration for rebar after yielding. Moreover, the restrained effect of rebar on free shrinkage of CA-UHPC leads to a decrease in the first cracking strength for R-CA-UHPC members. Based on the established development functions of elastic modulus, autogenous shrinkage, and tensile creep for CA-UHPC, the restrained effect was quantified according to Dischinger's-differential-equation-based theoretical analysis. Finally, the models to predict the first cracking stresses/strains and the yielding loads of the R-CA-UHPC members were developed and validated.

Bolted connections in prefabricated cantilevered cap beam-column structures: A comprehensive experimental and numerical study

Prefabricated cantilevered beam-column structures have gained popularity in mountainous road construction. However, a notable research gap exists regarding the beam-column connections. To fill this gap, a new bolted connection method was introduced for the cantilevered beam-column structure. The mechanical performance of this novel structure was systematically investigated through reduced-scale experiments and finite element analysis. The structure's response under three critical loading conditions was experimentally examined, revealing the efficacy of bolted connections in preserving structural integrity and highlighting pronounced strain concentration at the connection region of the cap beam. Furthermore, to enhance structural design, a finite element model of this beam-column structure was established to simulate the influence of various materials and structural parameters on structural response and damage. Simulation results indicated that the anchor rod angle and the number of bolts had a minor impact on load-bearing capacity and damage modes. In contrast, the choice of concrete material and steel reinforcement ratio significantly influenced load-bearing capacity. The insights derived from this study offer valuable guidance for optimizing and extending the application of this structural design.

Three‐Dimensional Electrical Resistivity Structure Beneath a Strain Concentration Area in the Back‐Arc Side of the Northeastern Japan Arc

AbstractIntraplate earthquakes occur more frequently in the Japanese islands than in other regions. Large intraplate earthquakes in the island arc preferentially occur in strain concentration zones detected by geological and geodetic studies. Crustal heterogeneity plays a crucial role in generating large intraplate earthquakes and strain concentrations. Thus, we elucidated the crustal heterogeneities beneath a strain concentration area on the back‐arc side of the northeastern Japan Arc based on electrical resistivity, which is sensitive to weak zones in the crust. By deploying wideband magnetotelluric surveys, we revealed the three‐dimensional electrical resistivity structure in the crust, suggesting the coexistence of two different types of strain‐concentration mechanisms in the strain‐concentration area. The shallow conductive layers and lower‐crustal conductors appear to act as low‐elastic‐modulus and low‐viscosity areas, respectively, and are responsible for the strain concentration. We found shallow and lower‐crustal conductors in the strain concentration zone revealed by geological studies. Those conductive areas are considered to act as mechanically weak zones and cause stress loading on the brittle pars of pre‐existing faults, resulting in large intraplate earthquakes. The resultant earthquakes presumably contribute to strain accumulation on a geological timescale. In addition, a spatial correlation between the epicenters of large intraplate earthquakes and edges of lower‐crustal conductors implies the contribution of the fluids in the lower crust to the generation of large earthquakes. We also identified vertical conductors ranging from the lower crust to Quaternary volcanoes, which may indicate trans‐crustal magmatic systems under these volcanoes.

Fabrication of TiB2/6061Al composite with tailoring heterostructure and superior mechanical properties and investigation on deformation behaviors

Overcoming the trade-off between strength and ductility remains a critical challenge for Al matrix composites. Here, TiB2/6061Al composites with harmonic and lamellar heterostructures were fabricated via hot press sintering by altering the size of Al powders. The discontinuous dynamic recrystallization (DDRX) and DRX take place, with DDRX being particularly promoted by the presence of fine grains around coarse grains. The activated slip systems of the grains with similar orientations are nearly identical during tension, while those of the randomly orientated grains vary significantly. Geometrically necessary dislocations form in fine grains due to their limited deformation compatibility, and also in the boundaries between fine and coarse grains. The heterostructured composite exhibits a superior strength-ductility synergy compared to the homogeneous composite, attributed to the hetero-deformation induced strengthening and dislocation strengthening. Strain concentrates in fine-grain zones with TiB2 particles, while the strain concentration is alleviated in coarse-grain zones, contributing to a high elongation.

SCFs for CHS-to-CHS T- and X-type moment-loaded end connections: Proposal for formulae

The research presented in this paper is part of a series of efforts to develop design recommendations for hollow structural section (HSS) connections situated at the end of a truss (or frame) under fatigue loading. Full-sized circular hollow section (CHS)-to-CHS regular and end connections (with open chord ends and chord-end cap plates) were tested under branch in-plane bending to measure strain concentrations at the critical locations. This experimental data was then used to verify a finite element (FE) modelling approach. An FE parametric study (totaling 1120 FE models, including 560 T-type connections and 560 X-type connections) was then carried out to investigate the effects of different chord end boundary conditions (i.e., varied chord end lengths, and open versus cap-plated chord ends). The effects of branch-to-chord width (β), chord slenderness (2γ) ratios and branch-to-chord thickness (τ) were also investigated. It was found that the stress concentration factors (SCFs) in end connections are sensitive to the boundary conditions (i.e., open chord end versus with chord-end cap plate) and all three non-dimensional parameters (β, 2γ and τ). Therefore, SCF correction factors based on e/b0, β, 2γ and τ, are derived for use with existing SCF formulae in CIDECT Design Guide 8.

Time-varying damage evolution of concrete under cyclic disturbance loading- an experimental observation utilizing AE and DIC

Cyclic disturbance loading affects the continuous operation of concrete structures. This study aims to better understand the failure behavior of concrete structures under cyclic disturbance loading. This study combined acoustic emission (AE) and digital image correlation (DIC) technologies to study mechanical failure behavior and damage evolution of concrete materials under cyclic disturbance loading and unloading. The AE event time-frequency characteristics, surface principal strain program, and damage parameters of samples with different cyclic disturbance amplitudes were obtained by theoretical calculation. The results reveal that when minor disturbance amplitudes are applied, the elastic modulus of concrete specimens tends to increase. When the disturbance amplitude is too large, the elastic modulus of the specimens drops, leading to faster specimen failure. The AE characteristic parameters exhibit a Felicity effect after the sample is subjected to a cyclic disturbance loading. Moreover, the larger the amplitude of the disturbance load, the more frequent the low-frequency and high-amplitude events generated during the final rupture, the higher the concentration of the principal strain on the sample's surface, and the wider the crack band distribution. AE energy and Effective crack are consistent in characterizing damage, and the changing trend of these two damage parameters can better reflect the deterioration process of concrete. The research results will provide practical and theoretical insights for safely operating and maintaining concrete structures.

Model test on the collapse mechanism of subway tunnels in the soil-sand-rock composite strata

Collapse is a typical type of accident during subway tunnel construction. Complex geological conditions, particularly soil-sand-rock composite strata, significantly contribute to strata instability, posing a serious threat to tunnel safety. Indeed, the progressive failure leading to the collapse of subway tunnels in these composite strata exhibits distinct characteristics from that of single strata and warrants further research. In this paper, model tests were conducted to investigate the collapse process of the soil-sand-rock composite strata under different overlayer rock thicknesses. Soil pressure sensors monitored the mechanical response of the strata, while a two-dimensional full-field deformation measurement and analysis system (XTDIC-2D) provided displacement and strain fields. Additionally, an industrial camera captured video footage of the failure process. The results demonstrated that the collapse characteristics of the soil-sand-rock composite strata were significantly impacted by overlayer rock layer thickness. As the thickness of the rock layer decreased, the collapse easily expanded to the sand layer under a slight loading. The sand layer exhibited distinct behaviors with high compressibility, thixotropy, and flowability during the collapse process. The high compressibility of the sand layer before collapse resulted in strain concentration within it, thereby resisting the deformation of the rock strata. After the collapse of the rock strata, the thixotropic and flowability properties caused the collapse surface to expand in a funnel shape towards both sides. A stabilized stratigraphic arch could not be formed due to the arch foot being located above the sand layer, which cannot provide stable support. Overall, the results of this research offer valuable guidance for the prevention and control of tunnel collapse in soil-sand-rock composite strata.

Subchondral bone fatigue injury in the parasagittal condylar grooves of the third metacarpal bone in thoroughbred racehorses elevates site-specific strain concentration

Condylar stress fracture of the distal end of the third metacarpal/metatarsal (MC3/MT3) bones is a major cause of Thoroughbred racehorse injury and euthanasia worldwide. Functional adaptation to exercise and fatigue damage lead to structural changes in the subchondral bone that include increased modeling (resulting in sclerotic bone tissue) and targeted remodeling repair (resulting in focal resorption spaces in the parasagittal groove). Whether these focal structural changes, as detectable by standing computed tomography (sCT), lead to elevated strain at the common site of condylar stress fracture has not been demonstrated. Therefore, the goal of the present study was to compare full-field three-dimensional (3D) strain on the distopalmar aspect of MC3 bone specimens with and without focal subchondral bone injury (SBI). Thirteen forelimb specimens were collected from racing Thoroughbreds for mechanical testing ex vivo and underwent sCT. Subsequently, full-field displacement and strain at the joint surface were determined using stereo digital image correlation. Strain concentration was observed in the parasagittal groove (PSG) of the loaded condyles, and those with SBI in the PSG showed higher strain rates in this region than control bones. PSG strain rate in condyles with PSG SBI was more sensitive to CT density distribution in comparison with condyles with no sCT-detectable injury. Findings from this study help to interpret structural changes in the subchondral bone due to fatigue damage and to assess risk of incipient stress fracture in a patient-specific manner.

Development of new improved plastic collapse moment equations of pressurized different angled pipe bends under bending moments

In a piping system, pipe bends are more flexible than straight pipes because of their curved geometry, supplemented by higher stress and strain concentration, leading to one of the crucial components in piping industries. Therefore, safe design of pipe bends is essential for smooth running of the piping system, and plastic collapse moment is one of its criteria. This paper utilizes three-dimensional finite element analyses to model empirical solutions for the plastic collapse moment for different angled pipe bends subjected to combined pressure and in-plane closing, in-plane opening, and out-of-plane bending moments. Plastic collapse moments for 30∘ to 180∘ pipe bends are determined for elastic perfectly plastic and strain hardening materials, employing large geometry change option and internal pressure effect. It is observed from results that pressure effect is more prominent in thinner pipe bends of larger bend angle under all bending cases. For in-plane opening and out-of-plane bending moments, collapse moment increases and then decreases with increase in pressure intensity for all sizes of pipe bend. However, for in-plane opening bending moment, collapse moments keep on decreasing for thicker ([Formula: see text] = 11.33) pipe bends. Finally, the study presents new improved plastic collapse moment solutions for different angled pipe bends under bending moment and internal pressure, derived from the finite element results of elastic perfectly plastic and strain hardening material models.

Effect of angled abutments in the posterior maxillary region on tilted implants: a 3D finite element analysis.

The aim of this study was to evaluate the bone tissue effects under dynamic loading using finite element analysis (FEA) for four angled abutments with different deviated palatal lateral tilt angles. A three-dimensional model of the posterior maxillary region and an implant crown model were reconstructed and assembled with a three-dimensional model of the implant, angled abutment, and central screw to create a total of 10 three-dimensional finite element models tilted at , , , and in three groups, and the dynamic loads simulating oral mastication were loaded on the implant crown to analyze the equivalent stresses and strains in the peri-implant bone tissues. Under the dynamic loading, the cortical bone on the buccal side of the implant neck showed different degrees of stress concentration, and the cortical bone stress was much higher than the cancellous bone, and the strain concentration area of each model was located in the bone tissue around the implant neck and base. For the use of angular abutment, under the premise that the cortical bone stresses and strains of the 10 models meet the requirements for use, the peak stresses of 2.907 MPa, 3.018 MPa, and 2.164 MPa were achieved by using the angular abutment to achieve the tilt angles of , , and implantation, which is more advantageous compared with other models.

Comparative Analysis of Effect of Culture Conditions on Growth and C-Phycocyanin Production in Helical and Linear Spirulina.

Spirulina (Arthrospira and Spirulina spp.) has always been characterized by the helical trichomes, despite the existence of linear forms. A great debate is now open on the morphological flexibility of Spirulina, but it seems that both trichome morphology and C-phycocyanin (C-PC) concentrations are influenced by the culture conditions.This work compared the effect of some key growth factors (medium pH as well as its carbon, potassium, and salt contents) on the growth and C-PC concentration of helical and linear Spirulina strains. Further, two-phase strategies, including light and nitrogen variation, were applied to increase the in vivo C-PC accumulation into the trichomes. Results showed that high pH induced trichomes elongation and improved growth but decreased C-PC content (+ 65 and + 43% vs. -83 and -49%, for helical and linear strains, respectively). Variations in carbon and salt concentrations negatively impacted growth and C-PC content, even if the linear strain was more robust against these fluctuations. It was also interesting to see that potassium increasing improved growth and C-PC content for both strains.The variation of light wavelength during the enrichment phase (in the two-phase strategy) improved by 50% C-PC accumulation in trichomes, especially after blue lighting for 96 h. Similar result was obtained after 48 h of nitrogen reduction, while its removal from the medium caused trichomes disintegration. The current work highlights the robustness of linear Spirulina strain and presents an efficient and scalable way to increase C-PC in vivo without affecting growth.