Concentration Of Potato Virus Research Articles

A reevaluation of bromoethane in comparison to rindite for the post-harvest detection of potato virus Y in tubers by ELISA

A reevaluation of breaking tuber dormancy with bromoethane to increase the concentration of potato virus Y in the tuber revealed a positive response, by ELISA testing, to the treatment. The degree of response increased with the maturity of the tuber. Response to treatment with rindite was generally stronger, although differences were slight.

Relationship of virus concentration with the field resistance to potato virus Y in potatoes

The concentration of potato virus Y (PVY) was determined, using ELISA values (A405 nm), in twenty-six potato cultivars belonging to five resistance groups, grown in the field and in the greenhouse. On the basis of virus concentration, potato cultivars of group A, B, and C did not differ significantly and constitute the most susceptible group; those of group D and E differ significantly with each other and with group A, B, C, and constitute moderate and highly resistant groups, respectively. In the second year of infection, virus concentration was higher in each group, irrespective of resistance level. Thus, the infected plants of resistant groups, in a second year of growth, could be as rich sources of virus to aphids as plants from susceptible groups.

The Effect of the Date of Rindite Treatment on the Concentration of Potato Virus Y in Tubers

AbstractThe concentration of potato virus Y (PVY) in tubers after artificial break of dormancy by Rindite treatment at three different dates (September 8, November 10, and January 11) after harvest (August 25, 1983) was examined by enzyme‐linked immuno‐sorbent assay (ELISA). The increase of virus concentration was more rapid after the early Rindite treatment than after the later ones. The virus concentration observed after the second and the third treatment varied among the cultivars and reached maximum values considerably later than after the first treatment. There seems to be a direct effect of the Rindite treatment on PVY multiplication in tubers.

Dioxohexahydrotriazin, eine neue vollsynthetische antiphytovirale Verbindung

This paper reports the first results of studies made to determine the antiviral activity of dioxo hexahydro triazine, a newly developed antiphytoviral compound. After treatment with dioxo hexahydro triazine, the concentration of helical potato virus X in leaves of Nicotiana tabacum "Samsun" was found to be considerably reduced in both the serological test and local lesion test conducted on Nicotiana glutinosa at temperatures of 14 degrees C +/- 3 degrees C. In secondarily infected leaves, the reduction of the concentration of potato virus X in the range of from 1 mol/l to 10(-3) mol/l was so that it was no longer possible for viruses to be detected serologically. In vitro, however, dioxo hexahydro triazine, which was added to the inoculum, did not produce a significant change in the activity of the virion of potato virus X. Therefore, it is reasonable to assume that dioxo hexahydro triazine tends to affect replication steps of viruses. The use of dioxo hexahydro triazine also had the effect of greatly and highly significantly reducing the concentration of the isodiametric cucumber mosaic virus.

Der Einfluß von Gibberellinsäure, 2-Chloräthylphosphonsäure und einigen Wachstumsretardantien auf die Virusvermehrung in systemisch infizierten Pflanzen : The Effect of Gibberellic Acid, 2-chlorethyl Phosphonic Acid, and some Growth Retardants on Virus Multiplication in Systemically Infected Plants

1. The influence of the growth regulators on virus multiplication was dependent, inter alia, upon the interplay of plant-specific growth regulators, which may be considerably altered by decapitation. 2. None of the growth regulators included in these studies was found to greatly affect the multiplication of potato virus X (PVX) in inoculated (basal) leaves of intact plants. However, multiplication was increased significantly in inoculated leaves of decapitated plants. 3. In secondarily infected apical leaves of intact plants the multiplication of PYX was only inconsiderably influenced by GA 3 , greatly and sometimes even highly significantly reduced by CCC, MH and CEP, and increased by BMH. In decapitated plants the virus concentration was increased not only by BMH, but also by GA 3 and CCC, and was reduced by MH. 4. The variation in concentration of potato virus Y (PVY) induced by the growth regulators was usually greater than that of PYX, while that of tobacoo mosaic virus (TMV) was normally smaller than that of PYX.

Inhibitory ability of sap from different tobacco varieties on potato virus Y

In these experiments the inhibitory ability of 12 varieties ofNicotiana tabacum L. to potato virus Y was studied by means of drop serological precipitation method. In 11 varieties under study a correlation was found between field resistance and the inhibitory ability of sap. Varieties S 390/1, line 40, fixed A 1 exhibited low inhibitory ability of sap, lines Bel 61-10, R 3, Criolo and Burley N-15 were of medium inhibitory ability and high inhibitory ability was found in varieities Viržinský zlatý, line 37, A 5205 and R 1. The variety Viržinský zlatý, which is believed to be susceptible to potato virus Y was found to have high inhibitory ability. This was confirmed by inoculation of leavesChenopodium giganteumDon., determination of the number of lesions by the half leaf test, and determination of relative concentration of potato virus Y by means of serological drop method. None of the varieties under study showed extremely high inhibitory ability against potato virus Y.

Effect of iron on the concentration of potato virus X in tomato

Results obtained from these experiments with tomato, inoculated with the ringspot strain of PVX, and supplied with 0.05 and 0.10 ppm of iron showed deficiency symptoms and an increase in growth of host plants up to the optimum level of 1.00 ppm. The inoculated plants showed less growth than non-inoculated ones which was evident from the height and fresh weight of the plants. The virus concentration, as measured by the local lesion count method, showed less virus at low levels of iron than at high levels. There was no significant difference either in plant growth or virus concentration between the excess level of iron (3.00 ppm) and the low level. Iron seemed to be a limiting factor for virus multiplication rather than for plant growth at deficiency levels.